FvH4_7g32970

Protein TRANSPARENT TESTA GLABRA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006319
Physical Location & Seq
Reverse (-)
23584148 .. 23586806
2659 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g32970.t1

Sequence Viewer

Length: 1035 bp
ATGGAGAATTCGACCCTCGAATCCCACCTCGGTCCCGAAAACTCCGTCACCTACGACTCCCCCTACCCTCTCTACGCCATGGCATTCTCCCCCGCCGCCCGCGCCCGCCACCACCACCCCAAAATCGCCGTCGGCAGCTTCATCGAGGAGTTCTCCAACCGGGTCGACATTCTCTCCTTCGACCCGGACACCCTAACCCTAAAACCCAATCCTTCTCTCTCCTTTGACCACCCCTACCCTCCCACCAAGCTCATGTTCCACCCAAACCCCAACACCCTCCACAAGTCCCACGACATCCTCGCCTCCTCCGGCGACTACCTCCGCCTCTGGGAGGTCAAAGATTCCTCCGTTGACCGCCTCGAGCCAATTTCGGTTCTCAACAACTCCAAAACCTCCGAGTTCTGCGCGCCGCTGACGTCATTCGACTGGAACGAGATCGAGCCGCGGCGGATCGGGACCTCCAGCATCGACACGACGTGCACGATCTGGGACATCGAGAAAGGCGTCGTCGAGACGCAGCTGATCGCGCACGACAAGGAGGTCTACGACATCGCGTGGGGCGAGGCTAGGGTTTTCGCGTCGGTCTCCGCCGACGGATCGGTGAGAATATTTGATCTGAGAGATAAAGAACACTCGACGATCATATACGAGAGTCCTCAGCCGGACACGCCGCTGCTCCGATTGGCGTGGAACAAGCAGGACTTGAGGTACATGGCGACGATTTTGATGGATAGTAACAAGGTTGTGATACTGGATATTCGGTCGCCGACGATGCCGGTGGCGGAGCTGGAGAGGCACAGAGGGAGTGTGAATGCGATTGCGTGGGCGCCGCAGAGCGCCAGGCATATTTGCTCGGGTGGGGATGACTCGCAGGCGCTGATTTGGGAGCTGCCGACGGTGGCGGGGCCGAATGGGATTGATCCTATGTCGATGTACTCGGCTGGCGCCGAGATTAATCAGCTGCAGTGGTCTGCTGGGCAGCCGGATTGGATTGCCATTGCGTTTTCGAACAAGATGCAGCTCTTGAAGGTTTGA

Protein Analysis

345

Amino Acids

38.5

Weight (kDa)

5.12

Isoelectric Point (pI)

53.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EIPR1 PF23609 41 - 295 6.3e-07 EIPR1 beta-propeller
Beta-prop_TEP1_2nd PF25047 92 - 210 7.4e-06 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 101 - 327 8.9e-20 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 134 - 337 3.7e-10 WDHD1 first WD40 domain
WD40_Prp19 PF24814 139 - 215 8.1e-08 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 140 - 278 6.3e-15 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 149 - 212 1.2e-06 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 149 - 209 5.6e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 150 - 211 2.8e-09 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 150 - 218 7.6e-06 CAF1B/HIR1 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 539
AatII GACGTC 1 cut(s) 419
AccB1I GGYRCC 2 cut(s) 826, 944
AccI GTMKAC 2 cut(s) 165, 543
AccII CGCG 6 cut(s) 102, 407, 445, 527, 554, 578
AclWI GGATC 3 cut(s) 458, 604, 914
AcsI RAATTY 1 cut(s) 7
AcyI GRCGYC 4 cut(s) 416, 504, 827, 945
AdeI CACNNNGTG 1 cut(s) 477
AfaI GTAC 2 cut(s) 710, 935
AfiI CCNNNNNNNGG 2 cut(s) 328, 331
AgsI TTSAA 1 cut(s) 1027
AjiI CACGTC 1 cut(s) 477
AjnI CCWGG 1 cut(s) 839
AjuI GAANNNNNNNTTGG 2 cut(s) 239, 271
AluBI AGCT 7 cut(s) 138, 250, 520, 787, 889, 961, 1021
AluI AGCT 7 cut(s) 138, 250, 520, 787, 889, 961, 1021
Alw21I GWGCWC 1 cut(s) 482
Alw26I GTCTC 2 cut(s) 506, 589
Alw44I GTGCAC 1 cut(s) 478
AlwI GGATC 3 cut(s) 458, 604, 914
AlwNI CAGNNNCTG 1 cut(s) 877
Ama87I CYCGRG 2 cut(s) 359, 853
AoxI GGCC 1 cut(s) 905
ApaLI GTGCAC 1 cut(s) 478
ApeKI GCWGC 7 cut(s) 135, 517, 673, 889, 961, 979, 1018
ApoI RAATTY 1 cut(s) 7
ArsI GACNNNNNNTTYG 4 cut(s) 30, 62, 114, 146
AseI ATTAAT 1 cut(s) 954
AspLEI GCGC 8 cut(s) 104, 407, 409, 529, 829, 839, 877, 947
AspS9I GGNCC 3 cut(s) 32, 456, 905
AsuC2I CCSGG 2 cut(s) 161, 185
AsuHPI GGTGA 2 cut(s) 40, 613
AsuII TTCGAA 1 cut(s) 1007
AvaI CYCGRG 2 cut(s) 359, 853
AvaII GGWCC 2 cut(s) 32, 456
BaeGI GKGCMC 1 cut(s) 482
BanI GGYRCC 2 cut(s) 826, 944
Bbv12I GWGCWC 1 cut(s) 482
BbvCI CCTCAGC 1 cut(s) 657
BbvI GCAGC 7 cut(s) 147, 529, 660, 876, 948, 991, 1030
BccI CCATC 1 cut(s) 721
BceAI ACGGC 1 cut(s) 113
BcgI CGANNNNNNTGC 4 cut(s) 124, 158, 997, 1031
BciT130I CCWGG 1 cut(s) 841
BcnI CCSGG 2 cut(s) 161, 185
BcoDI GTCTC 2 cut(s) 506, 589
BfaI CTAG 1 cut(s) 567
BfmI CTRYAG 1 cut(s) 962
BfoI RGCGCY 4 cut(s) 830, 840, 878, 948
Bme1390I CCNGG 3 cut(s) 161, 185, 841
Bme18I GGWCC 2 cut(s) 32, 456
BmeT110I CYCGRG 2 cut(s) 359, 853
BmgBI CACGTC 1 cut(s) 477
BmgT120I GGNCC 3 cut(s) 32, 456, 905
BmiI GGNNCC 5 cut(s) 34, 457, 828, 906, 946
BmrFI CCNGG 3 cut(s) 161, 185, 841
BmsI GCATC 3 cut(s) 474, 762, 1005
BplI GAGNNNNNCTC 2 cut(s) 137, 169
BpmI CTGGAG 2 cut(s) 445, 809
Bpu10I CCTNAGC 1 cut(s) 657
Bpu14I TTCGAA 1 cut(s) 1007
BpuEI CTTGAG 1 cut(s) 724
BpuMI CCSGG 2 cut(s) 161, 185
BsaHI GRCGYC 4 cut(s) 416, 504, 827, 945
BsaI GGTCTC 1 cut(s) 589
BsaJI CCNNGG 3 cut(s) 28, 78, 443
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bsc4I CCNNNNNNNGG 2 cut(s) 328, 331
Bse118I RCCGGY 1 cut(s) 775
Bse1I ACTGG 2 cut(s) 431, 756
Bse3DI GCAATG 1 cut(s) 996
BseBI CCWGG 1 cut(s) 841
BseDI CCNNGG 3 cut(s) 28, 78, 443
BseGI GGATG 2 cut(s) 294, 868
BseLI CCNNNNNNNGG 2 cut(s) 328, 331
BseMI GCAATG 1 cut(s) 996
BseMII CTCAG 2 cut(s) 608, 671
BseNI ACTGG 2 cut(s) 431, 756
BsePI GCGCGC 1 cut(s) 405
BseRI GAGGAG 2 cut(s) 161, 295
BseSI GKGCMC 1 cut(s) 482
BseXI GCAGC 7 cut(s) 147, 529, 660, 876, 948, 991, 1030
BseYI CCCAGC 1 cut(s) 974
Bsh1236I CGCG 6 cut(s) 102, 407, 445, 527, 554, 578
Bsh1285I CGRYCG 1 cut(s) 764
BshFI GGCC 1 cut(s) 907
BshNI GGYRCC 2 cut(s) 826, 944
BsiEI CGRYCG 1 cut(s) 764
BsiHKAI GWGCWC 1 cut(s) 482
BsiHKCI CYCGRG 2 cut(s) 359, 853
BsiSI CCGG 6 cut(s) 160, 185, 309, 662, 776, 983
BslFI GGGAC 4 cut(s) 18, 271, 469, 503
BslI CCNNNNNNNGG 2 cut(s) 328, 331
BsmAI GTCTC 2 cut(s) 506, 589
BsmBI CGTCTC 1 cut(s) 506
BsmFI GGGAC 4 cut(s) 18, 271, 469, 503
BsmI GAATGC 2 cut(s) 83, 817
BsnI GGCC 1 cut(s) 907
Bso31I GGTCTC 1 cut(s) 589
BsoBI CYCGRG 2 cut(s) 359, 853
Bsp119I TTCGAA 1 cut(s) 1007
Bsp1286I GDGCHC 1 cut(s) 482
Bsp143I GATC 8 cut(s) 435, 450, 483, 522, 596, 613, 639, 919
Bsp19I CCATGG 1 cut(s) 78
BspANI GGCC 1 cut(s) 907
BspCNI CTCAG 2 cut(s) 609, 670
BspFNI CGCG 6 cut(s) 102, 407, 445, 527, 554, 578
BspLI GGNNCC 5 cut(s) 34, 457, 828, 906, 946
BspMAI CTGCAG 1 cut(s) 966
BspPI GGATC 3 cut(s) 458, 604, 914
BspT104I TTCGAA 1 cut(s) 1007
BspT107I GGYRCC 2 cut(s) 826, 944
BspTNI GGTCTC 1 cut(s) 589
BsrDI GCAATG 1 cut(s) 996
BsrFI RCCGGY 1 cut(s) 775
BsrI ACTGG 2 cut(s) 431, 756
BssAI RCCGGY 1 cut(s) 775
BssECI CCNNGG 3 cut(s) 28, 78, 443
BssHII GCGCGC 1 cut(s) 405
BssMI GATC 8 cut(s) 435, 450, 483, 522, 596, 613, 639, 919
BssNI GRCGYC 4 cut(s) 416, 504, 827, 945
BssT1I CCWWGG 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 841
Bst4CI ACNGT 1 cut(s) 898
BstACI GRCGYC 4 cut(s) 416, 504, 827, 945
BstBI TTCGAA 1 cut(s) 1007
BstC8I GCNNGC 5 cut(s) 100, 106, 407, 873, 943
BstDEI CTNAG 2 cut(s) 617, 657
BstDSI CCRYGG 2 cut(s) 78, 443
BstENI CCTNNNNNAGG 1 cut(s) 329
BstF5I GGATG 2 cut(s) 294, 868
BstFNI CGCG 6 cut(s) 102, 407, 445, 527, 554, 578
BstH2I RGCGCY 4 cut(s) 830, 840, 878, 948
BstHHI GCGC 8 cut(s) 104, 407, 409, 529, 829, 839, 877, 947
BstKTI GATC 8 cut(s) 438, 453, 486, 525, 599, 616, 642, 922
BstMAI GTCTC 2 cut(s) 506, 589
BstMBI GATC 8 cut(s) 435, 450, 483, 522, 596, 613, 639, 919
BstMCI CGRYCG 1 cut(s) 764
BstMWI GCNNNNNNNGC 5 cut(s) 101, 526, 667, 772, 793
BstNI CCWGG 1 cut(s) 841
BstSCI CCNGG 3 cut(s) 159, 183, 839
BstSFI CTRYAG 1 cut(s) 962
BstSLI GKGCMC 1 cut(s) 482
BstUI CGCG 6 cut(s) 102, 407, 445, 527, 554, 578
BstV1I GCAGC 7 cut(s) 147, 529, 660, 876, 948, 991, 1030
BsuRI GGCC 1 cut(s) 907
BtgI CCRYGG 2 cut(s) 78, 443
BtgZI GCGATG 1 cut(s) 535
BtrI CACGTC 1 cut(s) 477
BtsCI GGATG 2 cut(s) 294, 868
BtsI GCAGTG 1 cut(s) 971
BtsIMutI CAGTG 1 cut(s) 971
Cac8I GCNNGC 5 cut(s) 100, 106, 407, 873, 943
CaiI CAGNNNCTG 1 cut(s) 877
CfoI GCGC 8 cut(s) 104, 407, 409, 529, 829, 839, 877, 947
Cfr10I RCCGGY 1 cut(s) 775
Cfr13I GGNCC 3 cut(s) 32, 456, 905
Cfr42I CCGCGG 1 cut(s) 446
CseI GACGC 3 cut(s) 493, 523, 567
Csp6I GTAC 2 cut(s) 709, 934
CviAII CATG 3 cut(s) 79, 253, 712
CviQI GTAC 2 cut(s) 709, 934
DdeI CTNAG 2 cut(s) 617, 657
DinI GGCGCC 2 cut(s) 828, 946
DpnI GATC 8 cut(s) 437, 452, 485, 524, 598, 615, 641, 921
DpnII GATC 8 cut(s) 435, 450, 483, 522, 596, 613, 639, 919
DraIII CACNNNGTG 1 cut(s) 477
DrdI GACNNNNNNGTC 1 cut(s) 539
DseDI GACNNNNNNGTC 1 cut(s) 539
EciI GGCGGA 4 cut(s) 311, 463, 577, 797
Eco130I CCWWGG 1 cut(s) 78
Eco31I GGTCTC 1 cut(s) 589
Eco47I GGWCC 2 cut(s) 32, 456
Eco88I CYCGRG 2 cut(s) 359, 853
EcoNI CCTNNNNNAGG 1 cut(s) 329
EcoO109I RGGNCCY 1 cut(s) 456
EcoRI GAATTC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 839
EcoT14I CCWWGG 1 cut(s) 78
EgeI GGCGCC 2 cut(s) 828, 946
EheI GGCGCC 2 cut(s) 828, 946
ErhI CCWWGG 1 cut(s) 78
Esp3I CGTCTC 1 cut(s) 506
FaeI CATG 3 cut(s) 82, 256, 715
FaiI YATR 7 cut(s) 80, 254, 644, 646, 713, 846, 926
FalI AAGNNNNNCTT 2 cut(s) 686, 718
FaqI GGGAC 4 cut(s) 18, 271, 469, 503
FatI CATG 3 cut(s) 78, 252, 711
FauI CCCGC 4 cut(s) 100, 107, 113, 895
FblI GTMKAC 2 cut(s) 165, 543
FokI GGATG 2 cut(s) 281, 875
FspBI CTAG 1 cut(s) 567
GlaI GCGC 8 cut(s) 103, 406, 408, 528, 828, 838, 876, 946
GsaI CCCAGC 1 cut(s) 978
GsuI CTGGAG 2 cut(s) 445, 809
HaeII RGCGCY 4 cut(s) 830, 840, 878, 948
HaeIII GGCC 1 cut(s) 907
HapII CCGG 6 cut(s) 160, 185, 309, 662, 776, 983
HgaI GACGC 3 cut(s) 493, 523, 567
HhaI GCGC 8 cut(s) 104, 407, 409, 529, 829, 839, 877, 947
Hin1I GRCGYC 4 cut(s) 416, 504, 827, 945
Hin1II CATG 3 cut(s) 82, 256, 715
Hin6I GCGC 8 cut(s) 102, 405, 407, 527, 827, 837, 875, 945
HinP1I GCGC 8 cut(s) 102, 405, 407, 527, 827, 837, 875, 945
HincII GTYRAC 2 cut(s) 166, 352
HindII GTYRAC 2 cut(s) 166, 352
HinfI GANTC 5 cut(s) 20, 56, 341, 652, 866
HpaII CCGG 6 cut(s) 160, 185, 309, 662, 776, 983
HphI GGTGA 2 cut(s) 40, 613
Hpy166II GTNNAC 4 cut(s) 166, 352, 480, 544
Hpy188I TCNGA 3 cut(s) 397, 618, 680
Hpy188III TCNNGA 5 cut(s) 35, 454, 496, 511, 1024
Hpy8I GTNNAC 4 cut(s) 166, 352, 480, 544
HpyAV CCTTC 3 cut(s) 187, 222, 1021
HpyCH4III ACNGT 1 cut(s) 898
HpyCH4IV ACGT 2 cut(s) 416, 476
HpyCH4V TGCA 3 cut(s) 480, 964, 1018
HpyF10VI GCNNNNNNNGC 5 cut(s) 101, 526, 667, 772, 793
HpyF3I CTNAG 2 cut(s) 617, 657
HpySE526I ACGT 2 cut(s) 416, 476
Hsp92I GRCGYC 4 cut(s) 416, 504, 827, 945
Hsp92II CATG 3 cut(s) 82, 256, 715
HspAI GCGC 8 cut(s) 102, 405, 407, 527, 827, 837, 875, 945
KasI GGCGCC 2 cut(s) 826, 944
KspI CCGCGG 1 cut(s) 446
Kzo9I GATC 8 cut(s) 435, 450, 483, 522, 596, 613, 639, 919
LmnI GCTCC 3 cut(s) 681, 784, 886
Lsp1109I GCAGC 7 cut(s) 147, 529, 660, 876, 948, 991, 1030
LweI GCATC 3 cut(s) 474, 762, 1005
MaeI CTAG 1 cut(s) 567
MaeII ACGT 2 cut(s) 416, 476
MaeIII GTNAC 2 cut(s) 46, 734
MalI GATC 8 cut(s) 437, 452, 485, 524, 598, 615, 641, 921
MboI GATC 8 cut(s) 435, 450, 483, 522, 596, 613, 639, 919
MhlI GDGCHC 1 cut(s) 482
MluCI AATT 2 cut(s) 7, 366
Mly113I GGCGCC 2 cut(s) 827, 945
MlyI GAGTC 3 cut(s) 50, 661, 860
MmeI TCCRAC 1 cut(s) 180
MseI TTAA 1 cut(s) 954
MspA1I CMGCKG 5 cut(s) 412, 445, 520, 673, 961
MspI CCGG 6 cut(s) 160, 185, 309, 662, 776, 983
MspR9I CCNGG 3 cut(s) 161, 185, 841
Mva1269I GAATGC 2 cut(s) 83, 817
MvaI CCWGG 1 cut(s) 841
MvnI CGCG 6 cut(s) 102, 407, 445, 527, 554, 578
MwoI GCNNNNNNNGC 5 cut(s) 101, 526, 667, 772, 793
NarI GGCGCC 2 cut(s) 827, 945
NciI CCSGG 2 cut(s) 161, 185
NcoI CCATGG 1 cut(s) 78
NdeII GATC 8 cut(s) 435, 450, 483, 522, 596, 613, 639, 919
NlaIII CATG 3 cut(s) 82, 256, 715
NlaIV GGNNCC 5 cut(s) 34, 457, 828, 906, 946
NmeAIII GCCGAG 2 cut(s) 917, 973
NmuCI GTSAC 1 cut(s) 46
NspV TTCGAA 1 cut(s) 1007
PaeR7I CTCGAG 1 cut(s) 359
PauI GCGCGC 1 cut(s) 405
PcsI WCGNNNNNNNCGW 4 cut(s) 429, 479, 501, 558
PctI GAATGC 2 cut(s) 83, 817
PfeI GAWTC 2 cut(s) 20, 341
PleI GAGTC 3 cut(s) 50, 660, 860
PluTI GGCGCC 2 cut(s) 830, 948
PpsI GAGTC 3 cut(s) 50, 660, 860
PpuMI RGGWCCY 1 cut(s) 456
PshBI ATTAAT 1 cut(s) 954
Psp5II RGGWCCY 1 cut(s) 456
Psp6I CCWGG 1 cut(s) 839
PspFI CCCAGC 1 cut(s) 974
PspGI CCWGG 1 cut(s) 839
PspN4I GGNNCC 5 cut(s) 34, 457, 828, 906, 946
PspPI GGNCC 3 cut(s) 32, 456, 905
PspPPI RGGWCCY 1 cut(s) 456
PspXI VCTCGAGB 1 cut(s) 359
PstI CTGCAG 1 cut(s) 966
PstNI CAGNNNCTG 1 cut(s) 877
PteI GCGCGC 1 cut(s) 405
PvuII CAGCTG 2 cut(s) 520, 961
RsaI GTAC 2 cut(s) 710, 935
RsaNI GTAC 2 cut(s) 709, 934
SacII CCGCGG 1 cut(s) 446
SalI GTCGAC 1 cut(s) 164
SaqAI TTAA 1 cut(s) 954
Sau3AI GATC 8 cut(s) 435, 450, 483, 522, 596, 613, 639, 919
Sau96I GGNCC 3 cut(s) 32, 456, 905
SchI GAGTC 3 cut(s) 50, 661, 860
ScrFI CCNGG 3 cut(s) 161, 185, 841
SduI GDGCHC 1 cut(s) 482
SfaNI GCATC 3 cut(s) 474, 762, 1005
SfcI CTRYAG 1 cut(s) 962
SfoI GGCGCC 2 cut(s) 828, 946
Sfr274I CTCGAG 1 cut(s) 359
Sfr303I CCGCGG 1 cut(s) 446
SfuI TTCGAA 1 cut(s) 1007
SgrBI CCGCGG 1 cut(s) 446
SinI GGWCC 2 cut(s) 32, 456
SlaI CTCGAG 1 cut(s) 359
SmlI CTYRAG 2 cut(s) 359, 703
SmoI CTYRAG 2 cut(s) 359, 703
Sse9I AATT 2 cut(s) 7, 366
SspDI GGCGCC 2 cut(s) 826, 944
SspI AATATT 1 cut(s) 609
SspMI CTAG 1 cut(s) 567
StyD4I CCNGG 3 cut(s) 159, 183, 839
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 898
TaiI ACGT 2 cut(s) 419, 479
TaqII GACCGA 3 cut(s) 20, 571, 750
TasI AATT 2 cut(s) 7, 366
TatI WGTACW 1 cut(s) 933
TauI GCSGC 6 cut(s) 98, 412, 445, 448, 673, 832
TfiI GAWTC 2 cut(s) 20, 341
Tru1I TTAA 1 cut(s) 954
Tru9I TTAA 1 cut(s) 954
TscAI CASTG 1 cut(s) 971
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 7 cut(s) 135, 517, 673, 889, 961, 979, 1018
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 1 cut(s) 130
TspGWI ACGGA 3 cut(s) 34, 337, 609
TspRI CASTG 1 cut(s) 971
VneI GTGCAC 1 cut(s) 478
VpaK11BI GGWCC 2 cut(s) 32, 456
VspI ATTAAT 1 cut(s) 954
XagI CCTNNNNNAGG 1 cut(s) 329
XapI RAATTY 1 cut(s) 7
XhoI CTCGAG 1 cut(s) 359
XmiI GTMKAC 2 cut(s) 165, 543
XspI CTAG 1 cut(s) 567
ZraI GACGTC 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.