Rw1G040930

Protein TRANSPARENT TESTA GLABRA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0006319
Physical Location & Seq
Forward (+)
67997174 .. 68000612
3439 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G040930.1

Sequence Viewer

Length: 1032 bp
ATGGAGAACTCGACCCAAGAATCCCACCTCGGCCCGGAAAACTCGGTCACCTACGACTCCCCCTACCCGCTCTACGCCATGGCGTTTTCCCCGACCCGAACCCGCCACAACCACCCCCGCATCGCCGTCGGCAGCTTCATCGAGGAGTTCTCCAACCGGGTCGACATTCTCTCCTTCGACCCGGACACCCTGACCCTAAAGGCCAACCCTTCCCTCTCCTTCGACCACCCCTACCCTCCCACCAAGCTCATGTTCCACCCAAACCCTAACTCCCTCCACAAGTCCAACGACATCCTCGCCTCCTCCGGCGACTACCTCCGCCTCTGGGAGGTCAAAGACTCCTCCGTCGACCGCCTCGAGCCCATCTCCGTCCTCAACAACTCCAAGACCAGCGAGTTCTGCGCGCCGCTGACGTCATTCGACTGGAACGAGATCGAGCCCCGGCGAATCGGAACCTCCAGCATCGACACCACCTGTACGATTTGGGACATCGAGAAGGGCGTCGTCGAGACGCAGCTCATCGCTCACGATAAGGAGGTTTACGACATTGCGTGGGGCGAAGCTAGGGTTTTCGCCTCCGTCTCCGCCGACGGGTCGGTGAGGATTTTTGATCTGAGGGACAAGGAGCATTCCACGATTATATACGAGAGTCCTCAGCCGGACACGCCGCTGCTCCGGTTGGCCTGGAACAAGCAGGACCTGAGGTACATGGCGACGATTCTGATGGATAGCAACAAGGTCGTGATATTGGATATTCGATCGCCGACGATGCCGGTCGCGGAGCTGGAGAGGCACAGGGGCAGTGTGAATGCAATTGCGTGGGCGCCGCAGAGTGCTAGGCATATTTGTTCGGCTGGGGATGACTCGCAGGCGCTTATTTGGGAGCTGCCGACGGTGGCGGGGCCTAATGGGATTGATCCAATGTCAATGTACTCGGCCGGCGCAGAGATTAATCAGCTGCAGTGGTCTGCAGCGCAGCCGGATTGGATTGCCATTGCGTTTAGTAATAAGATGCAGCTGTTGAAGGTTTGA

Protein Analysis

343

Amino Acids

38.47

Weight (kDa)

5.0

Isoelectric Point (pI)

53.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EIPR1 PF23609 33 - 294 8.5e-08 EIPR1 beta-propeller
Beta-prop_TEP1_2nd PF25047 91 - 209 4.8e-06 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 93 - 326 2.7e-19 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 133 - 336 1.7e-10 WDHD1 first WD40 domain
WD40_Prp19 PF24814 138 - 214 7.3e-08 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 139 - 277 8.3e-15 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 148 - 211 1.3e-06 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 148 - 208 5.6e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 149 - 210 3.5e-09 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 149 - 217 7.7e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 161 - 298 5.8e-09 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 344
AatII GACGTC 1 cut(s) 416
AccB1I GGYRCC 1 cut(s) 823
AccBSI CCGCTC 1 cut(s) 70
AccI GTMKAC 2 cut(s) 162, 348
AccII CGCG 2 cut(s) 404, 779
AclWI GGATC 1 cut(s) 911
AcoI YGGCCR 1 cut(s) 936
AcyI GRCGYC 3 cut(s) 413, 501, 824
AfaI GTAC 3 cut(s) 478, 707, 932
AfiI CCNNNNNNNGG 4 cut(s) 34, 325, 328, 591
AgsI TTSAA 1 cut(s) 1024
AjnI CCWGG 1 cut(s) 683
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AluBI AGCT 8 cut(s) 135, 247, 517, 563, 784, 886, 958, 1018
AluI AGCT 8 cut(s) 135, 247, 517, 563, 784, 886, 958, 1018
Alw26I GTCTC 2 cut(s) 503, 586
AlwI GGATC 1 cut(s) 911
AlwNI CAGNNNCTG 1 cut(s) 700
Ama87I CYCGRG 1 cut(s) 356
AoxI GGCC 5 cut(s) 31, 201, 681, 902, 936
ApeKI GCWGC 8 cut(s) 132, 514, 670, 886, 958, 971, 976, 1015
AseI ATTAAT 1 cut(s) 951
AspLEI GCGC 6 cut(s) 404, 406, 826, 874, 944, 976
AspS9I GGNCC 3 cut(s) 32, 697, 902
AsuC2I CCSGG 4 cut(s) 35, 158, 182, 442
AsuHPI GGTGA 2 cut(s) 40, 610
AvaI CYCGRG 1 cut(s) 356
AvaII GGWCC 1 cut(s) 697
AxyI CCTNAGG 1 cut(s) 701
BanI GGYRCC 1 cut(s) 823
BanII GRGCYC 2 cut(s) 363, 441
BbvCI CCTCAGC 1 cut(s) 654
BbvI GCAGC 8 cut(s) 144, 526, 657, 873, 945, 983, 988, 1027
BccI CCATC 2 cut(s) 371, 718
BceAI ACGGC 1 cut(s) 110
BcgI CGANNNNNNTGC 6 cut(s) 109, 121, 143, 155, 721, 755
BciT130I CCWGG 1 cut(s) 685
BcnI CCSGG 4 cut(s) 35, 158, 182, 442
BcoDI GTCTC 2 cut(s) 503, 586
BfaI CTAG 2 cut(s) 564, 837
BfmI CTRYAG 2 cut(s) 959, 969
BfoI RGCGCY 2 cut(s) 827, 875
Bme1390I CCNGG 5 cut(s) 35, 158, 182, 442, 685
Bme18I GGWCC 1 cut(s) 697
BmeT110I CYCGRG 1 cut(s) 356
BmgT120I GGNCC 3 cut(s) 32, 697, 902
BmiI GGNNCC 3 cut(s) 454, 825, 903
BmrFI CCNGG 5 cut(s) 35, 158, 182, 442, 685
BmsI GCATC 4 cut(s) 129, 471, 759, 1002
BplI GAGNNNNNCTC 4 cut(s) 134, 166, 350, 382
BpmI CTGGAG 2 cut(s) 442, 806
Bpu10I CCTNAGC 1 cut(s) 654
BpuMI CCSGG 4 cut(s) 35, 158, 182, 442
BsaHI GRCGYC 3 cut(s) 413, 501, 824
BsaJI CCNNGG 3 cut(s) 28, 78, 440
BsaWI WCCGGW 1 cut(s) 675
BsaXI ACNNNNNCTCC 4 cut(s) 155, 185, 254, 284
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 325, 328, 591
Bse118I RCCGGY 2 cut(s) 772, 938
Bse1I ACTGG 1 cut(s) 428
Bse21I CCTNAGG 1 cut(s) 701
Bse3DI GCAATG 2 cut(s) 546, 993
BseBI CCWGG 1 cut(s) 685
BseDI CCNNGG 3 cut(s) 28, 78, 440
BseGI GGATG 2 cut(s) 291, 865
BseLI CCNNNNNNNGG 4 cut(s) 34, 325, 328, 591
BseMI GCAATG 2 cut(s) 546, 993
BseMII CTCAG 3 cut(s) 605, 668, 692
BseNI ACTGG 1 cut(s) 428
BsePI GCGCGC 1 cut(s) 402
BseRI GAGGAG 3 cut(s) 158, 292, 331
BseX3I CGGCCG 1 cut(s) 936
BseXI GCAGC 8 cut(s) 144, 526, 657, 873, 945, 983, 988, 1027
BseYI CCCAGC 1 cut(s) 854
Bsh1236I CGCG 2 cut(s) 404, 779
Bsh1285I CGRYCG 4 cut(s) 352, 761, 777, 939
BshFI GGCC 5 cut(s) 33, 203, 683, 904, 938
BshNI GGYRCC 1 cut(s) 823
BsiEI CGRYCG 4 cut(s) 352, 761, 777, 939
BsiHKCI CYCGRG 1 cut(s) 356
BslFI GGGAC 2 cut(s) 500, 632
BslI CCNNNNNNNGG 4 cut(s) 34, 325, 328, 591
BsmAI GTCTC 2 cut(s) 503, 586
BsmBI CGTCTC 2 cut(s) 503, 586
BsmFI GGGAC 2 cut(s) 500, 632
BsmI GAATGC 2 cut(s) 628, 814
BsnI GGCC 5 cut(s) 33, 203, 683, 904, 938
BsoBI CYCGRG 1 cut(s) 356
Bsp1286I GDGCHC 2 cut(s) 363, 441
Bsp143I GATC 4 cut(s) 432, 610, 758, 916
Bsp19I CCATGG 1 cut(s) 78
BspANI GGCC 5 cut(s) 33, 203, 683, 904, 938
BspCNI CTCAG 3 cut(s) 606, 667, 693
BspFNI CGCG 2 cut(s) 404, 779
BspLI GGNNCC 3 cut(s) 454, 825, 903
BspMAI CTGCAG 2 cut(s) 963, 973
BspPI GGATC 1 cut(s) 911
BspT107I GGYRCC 1 cut(s) 823
BsrBI CCGCTC 1 cut(s) 70
BsrDI GCAATG 2 cut(s) 546, 993
BsrFI RCCGGY 2 cut(s) 772, 938
BsrI ACTGG 1 cut(s) 428
BssAI RCCGGY 2 cut(s) 772, 938
BssECI CCNNGG 3 cut(s) 28, 78, 440
BssHII GCGCGC 1 cut(s) 402
BssMI GATC 4 cut(s) 432, 610, 758, 916
BssNI GRCGYC 3 cut(s) 413, 501, 824
BssT1I CCWWGG 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 685
Bst4CI ACNGT 1 cut(s) 895
BstACI GRCGYC 3 cut(s) 413, 501, 824
BstC8I GCNNGC 3 cut(s) 404, 870, 940
BstDEI CTNAG 3 cut(s) 614, 654, 701
BstDSI CCRYGG 1 cut(s) 78
BstEII GGTNACC 1 cut(s) 46
BstENI CCTNNNNNAGG 1 cut(s) 326
BstF5I GGATG 2 cut(s) 291, 865
BstFNI CGCG 2 cut(s) 404, 779
BstH2I RGCGCY 2 cut(s) 827, 875
BstHHI GCGC 6 cut(s) 404, 406, 826, 874, 944, 976
BstKTI GATC 4 cut(s) 435, 613, 761, 919
BstMAI GTCTC 2 cut(s) 503, 586
BstMBI GATC 4 cut(s) 432, 610, 758, 916
BstMCI CGRYCG 4 cut(s) 352, 761, 777, 939
BstMWI GCNNNNNNNGC 4 cut(s) 399, 664, 769, 790
BstNI CCWGG 1 cut(s) 685
BstPI GGTNACC 1 cut(s) 46
BstSCI CCNGG 5 cut(s) 33, 156, 180, 440, 683
BstSFI CTRYAG 2 cut(s) 959, 969
BstUI CGCG 2 cut(s) 404, 779
BstV1I GCAGC 8 cut(s) 144, 526, 657, 873, 945, 983, 988, 1027
BstZI CGGCCG 1 cut(s) 936
Bsu36I CCTNAGG 1 cut(s) 701
BsuRI GGCC 5 cut(s) 33, 203, 683, 904, 938
BtgI CCRYGG 1 cut(s) 78
BtgZI GCGATG 2 cut(s) 106, 505
BtsCI GGATG 2 cut(s) 291, 865
BtsI GCAGTG 2 cut(s) 808, 968
BtsIMutI CAGTG 2 cut(s) 808, 968
Cac8I GCNNGC 3 cut(s) 404, 870, 940
CaiI CAGNNNCTG 1 cut(s) 700
CfoI GCGC 6 cut(s) 404, 406, 826, 874, 944, 976
Cfr10I RCCGGY 2 cut(s) 772, 938
Cfr13I GGNCC 3 cut(s) 32, 697, 902
CseI GACGC 2 cut(s) 490, 520
Csp6I GTAC 3 cut(s) 477, 706, 931
CviAII CATG 3 cut(s) 79, 250, 709
CviQI GTAC 3 cut(s) 477, 706, 931
DdeI CTNAG 3 cut(s) 614, 654, 701
DinI GGCGCC 1 cut(s) 825
DpnI GATC 4 cut(s) 434, 612, 760, 918
DpnII GATC 4 cut(s) 432, 610, 758, 916
DrdI GACNNNNNNGTC 1 cut(s) 344
DseDI GACNNNNNNGTC 1 cut(s) 344
EaeI YGGCCR 1 cut(s) 936
EagI CGGCCG 1 cut(s) 936
EciI GGCGGA 2 cut(s) 308, 574
EclXI CGGCCG 1 cut(s) 936
Eco130I CCWWGG 1 cut(s) 78
Eco24I GRGCYC 2 cut(s) 363, 441
Eco47I GGWCC 1 cut(s) 697
Eco52I CGGCCG 1 cut(s) 936
Eco81I CCTNAGG 1 cut(s) 701
Eco88I CYCGRG 1 cut(s) 356
Eco91I GGTNACC 1 cut(s) 46
EcoNI CCTNNNNNAGG 1 cut(s) 326
EcoO109I RGGNCCY 2 cut(s) 697, 902
EcoO65I GGTNACC 1 cut(s) 46
EcoRII CCWGG 1 cut(s) 683
EcoT14I CCWWGG 1 cut(s) 78
EcoT38I GRGCYC 2 cut(s) 363, 441
EgeI GGCGCC 1 cut(s) 825
EheI GGCGCC 1 cut(s) 825
ErhI CCWWGG 1 cut(s) 78
Esp3I CGTCTC 2 cut(s) 503, 586
FaeI CATG 3 cut(s) 82, 253, 712
FaiI YATR 6 cut(s) 80, 251, 641, 643, 710, 843
FaqI GGGAC 2 cut(s) 500, 632
FatI CATG 3 cut(s) 78, 249, 708
FauI CCCGC 4 cut(s) 75, 110, 125, 892
FblI GTMKAC 2 cut(s) 162, 348
FokI GGATG 2 cut(s) 278, 872
FriOI GRGCYC 2 cut(s) 363, 441
FspBI CTAG 2 cut(s) 564, 837
GlaI GCGC 6 cut(s) 403, 405, 825, 873, 943, 975
GsaI CCCAGC 1 cut(s) 858
GsuI CTGGAG 2 cut(s) 442, 806
HaeII RGCGCY 2 cut(s) 827, 875
HaeIII GGCC 5 cut(s) 33, 203, 683, 904, 938
HgaI GACGC 2 cut(s) 490, 520
HhaI GCGC 6 cut(s) 404, 406, 826, 874, 944, 976
Hin1I GRCGYC 3 cut(s) 413, 501, 824
Hin1II CATG 3 cut(s) 82, 253, 712
Hin6I GCGC 6 cut(s) 402, 404, 824, 872, 942, 974
HinP1I GCGC 6 cut(s) 402, 404, 824, 872, 942, 974
HincII GTYRAC 2 cut(s) 163, 349
HindII GTYRAC 2 cut(s) 163, 349
HinfI GANTC 7 cut(s) 20, 56, 338, 447, 649, 718, 863
HphI GGTGA 2 cut(s) 40, 610
Hpy166II GTNNAC 3 cut(s) 163, 349, 541
Hpy188I TCNGA 3 cut(s) 452, 615, 723
Hpy188III TCNNGA 4 cut(s) 493, 508, 527, 742
Hpy8I GTNNAC 3 cut(s) 163, 349, 541
Hpy99I CGWCG 8 cut(s) 131, 350, 506, 509, 593, 718, 769, 895
HpyAV CCTTC 5 cut(s) 184, 219, 229, 490, 1018
HpyCH4III ACNGT 1 cut(s) 895
HpyCH4IV ACGT 1 cut(s) 413
HpyCH4V TGCA 4 cut(s) 812, 961, 971, 1015
HpyF10VI GCNNNNNNNGC 4 cut(s) 399, 664, 769, 790
HpyF3I CTNAG 3 cut(s) 614, 654, 701
HpySE526I ACGT 1 cut(s) 413
Hsp92I GRCGYC 3 cut(s) 413, 501, 824
Hsp92II CATG 3 cut(s) 82, 253, 712
HspAI GCGC 6 cut(s) 402, 404, 824, 872, 942, 974
KasI GGCGCC 1 cut(s) 823
KroI GCCGGC 1 cut(s) 938
KroNI GCCGGC 1 cut(s) 940
Kzo9I GATC 4 cut(s) 432, 610, 758, 916
LmnI GCTCC 4 cut(s) 625, 678, 781, 883
Lsp1109I GCAGC 8 cut(s) 144, 526, 657, 873, 945, 983, 988, 1027
LweI GCATC 4 cut(s) 129, 471, 759, 1002
MaeI CTAG 2 cut(s) 564, 837
MaeII ACGT 1 cut(s) 413
MaeIII GTNAC 1 cut(s) 46
MalI GATC 4 cut(s) 434, 612, 760, 918
MbiI CCGCTC 1 cut(s) 70
MboI GATC 4 cut(s) 432, 610, 758, 916
MfeI CAATTG 1 cut(s) 813
MhlI GDGCHC 2 cut(s) 363, 441
MluCI AATT 1 cut(s) 813
Mly113I GGCGCC 1 cut(s) 824
MlyI GAGTC 4 cut(s) 50, 332, 658, 857
MmeI TCCRAC 2 cut(s) 177, 309
MroNI GCCGGC 1 cut(s) 938
MseI TTAA 1 cut(s) 951
MspA1I CMGCKG 4 cut(s) 409, 670, 958, 1018
MspR9I CCNGG 5 cut(s) 35, 158, 182, 442, 685
MunI CAATTG 1 cut(s) 813
Mva1269I GAATGC 2 cut(s) 628, 814
MvaI CCWGG 1 cut(s) 685
MvnI CGCG 2 cut(s) 404, 779
MwoI GCNNNNNNNGC 4 cut(s) 399, 664, 769, 790
NaeI GCCGGC 1 cut(s) 940
NarI GGCGCC 1 cut(s) 824
NciI CCSGG 4 cut(s) 35, 158, 182, 442
NcoI CCATGG 1 cut(s) 78
NdeII GATC 4 cut(s) 432, 610, 758, 916
NgoMIV GCCGGC 1 cut(s) 938
NlaIII CATG 3 cut(s) 82, 253, 712
NlaIV GGNNCC 3 cut(s) 454, 825, 903
NmeAIII GCCGAG 2 cut(s) 9, 914
NmuCI GTSAC 1 cut(s) 46
PaeR7I CTCGAG 1 cut(s) 356
PauI GCGCGC 1 cut(s) 402
PcsI WCGNNNNNNNCGW 3 cut(s) 354, 426, 498
PctI GAATGC 2 cut(s) 628, 814
PdiI GCCGGC 1 cut(s) 940
PfeI GAWTC 3 cut(s) 20, 447, 718
Ple19I CGATCG 1 cut(s) 761
PleI GAGTC 4 cut(s) 50, 332, 657, 857
PluTI GGCGCC 1 cut(s) 827
PpsI GAGTC 4 cut(s) 50, 332, 657, 857
PpuMI RGGWCCY 1 cut(s) 697
PshBI ATTAAT 1 cut(s) 951
Psp5II RGGWCCY 1 cut(s) 697
Psp6I CCWGG 1 cut(s) 683
PspEI GGTNACC 1 cut(s) 46
PspFI CCCAGC 1 cut(s) 854
PspGI CCWGG 1 cut(s) 683
PspN4I GGNNCC 3 cut(s) 454, 825, 903
PspPI GGNCC 3 cut(s) 32, 697, 902
PspPPI RGGWCCY 1 cut(s) 697
PspXI VCTCGAGB 1 cut(s) 356
PstI CTGCAG 2 cut(s) 963, 973
PstNI CAGNNNCTG 1 cut(s) 700
PteI GCGCGC 1 cut(s) 402
PvuI CGATCG 1 cut(s) 761
PvuII CAGCTG 2 cut(s) 958, 1018
RsaI GTAC 3 cut(s) 478, 707, 932
RsaNI GTAC 3 cut(s) 477, 706, 931
SalI GTCGAC 2 cut(s) 161, 347
SaqAI TTAA 1 cut(s) 951
Sau3AI GATC 4 cut(s) 432, 610, 758, 916
Sau96I GGNCC 3 cut(s) 32, 697, 902
SchI GAGTC 4 cut(s) 50, 332, 658, 857
ScrFI CCNGG 5 cut(s) 35, 158, 182, 442, 685
SduI GDGCHC 2 cut(s) 363, 441
SfaNI GCATC 4 cut(s) 129, 471, 759, 1002
SfcI CTRYAG 2 cut(s) 959, 969
SfoI GGCGCC 1 cut(s) 825
Sfr274I CTCGAG 1 cut(s) 356
SinI GGWCC 1 cut(s) 697
SlaI CTCGAG 1 cut(s) 356
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
Sse9I AATT 1 cut(s) 813
SspDI GGCGCC 1 cut(s) 823
SspMI CTAG 2 cut(s) 564, 837
StyD4I CCNGG 5 cut(s) 33, 156, 180, 440, 683
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 895
TaiI ACGT 1 cut(s) 416
TaqII GACCGA 1 cut(s) 34
TasI AATT 1 cut(s) 813
TatI WGTACW 1 cut(s) 930
TauI GCSGC 3 cut(s) 409, 670, 829
TfiI GAWTC 3 cut(s) 20, 447, 718
Tru1I TTAA 1 cut(s) 951
Tru9I TTAA 1 cut(s) 951
TscAI CASTG 2 cut(s) 808, 968
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 8 cut(s) 132, 514, 670, 886, 958, 971, 976, 1015
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 1 cut(s) 127
TspGWI ACGGA 3 cut(s) 334, 358, 568
TspRI CASTG 2 cut(s) 808, 968
VpaK11BI GGWCC 1 cut(s) 697
VspI ATTAAT 1 cut(s) 951
XagI CCTNNNNNAGG 1 cut(s) 326
XhoI CTCGAG 1 cut(s) 356
XmiI GTMKAC 2 cut(s) 162, 348
XspI CTAG 2 cut(s) 564, 837
ZraI GACGTC 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.