Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0006319

Physical Location & Seq

Genomic Coordinates Reverse (-)

31831915 .. 31834625

Gene Structure 2711 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1228700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1029 bp

ATGGAGAACTCTACGCAAGAATCCCATCTCAGAGCGGAGAACTCGGTGACGTACGAGTCGCCGTACCCGCTGTACGCCATGGCCTTTGCCTCGCCCCAAACCCGAACCCGCCACCAGCACCACCGAATCGCCGTCGGCAGCTTTATCGAGGAGTACTCGAACCGGGTCGATATCCTTTCCTTCGACCCGGATACCCTTTCAATAAAGCCGAACCCGACCCTCTCCTTCGACCACCCTTATCCGCCCACCAAGCTCATGTTCCACCCGAACCCCAACGCCCTCCACAAAACCAACGACGTCTTAGCTTCCTCTGGCGACTACCTCCGCCTTTGGGAGGTCGGCGACTCCACCGTCGAGCCTATCCAGGTGTTAAACAACAGCAAGACCAGCGAGTTTTGCGCTCCGCTGACGTCATTCGATTGGAACGACATTGAGCCCCGGCGAATTGGGACTTCCAGCATCGACACCACCTGCACAATTTGGGACATCGAAAAGGGTGTCGTCGAGACGCAGCTGATTGCTCACGATAAAGAGGTGTACGACATCGCGTGGGGGGAAGCTAGGGTTTTTGCTTCGGTTTCGGCTGATGGGTCGGTGAGAATTTTCGATTTGAGGGACAAGGAGCACTCCACGATCATCTATGAGAGCCCTCAGCCGGATACCCCTTTGCTTCGATTGGCTTGGAACAAGCAGGATTTGAGGTACATGGCCACAATTTTGATGGACAGCAATAAAGTTGTGATCTTGGATATCCGATCGCCGACGATGCCAGTGGCGGAGCTGGAGAGGCACAGAGGTAGTGTGAATGCTATTGCTTGGGCCCCCCAGAGTTGTAGGCACATTTGCTCTGCTGGGGATGACACTCAGGCGCTTATTTGGGACCTGCCCACGGTCGCTGGGCCGAATGGAATCGACCCCATGTCGATGTACTCCGCAGGTGCGGAGATTAATCAGCTGCAGTGGTCTGCTGCACAGCCTGATTGGATTTCCATTGCATTTTCTAACAAGATGCAGCTTTTGAAGGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0008150 GO:0008544 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009888 GO:0009913 GO:0009957 GO:0009987 GO:0010026 GO:0010033 GO:0030154 GO:0030855 GO:0032502 GO:0032879 GO:0032880 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045165 GO:0048856 GO:0048869 GO:0050789 GO:0050896 GO:0060429 GO:0065007 GO:0090558 GO:0097159 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

343

Amino Acids

38.47

Weight (kDa)

5.05

Isoelectric Point (pI)

49.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	96 - 329	4e-19	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	134 - 336	2e-10	WDHD1 first WD40 domain
WD40_Prp19	PF24814	137 - 213	8.3e-08	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	138 - 298	7.4e-17	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	146 - 211	8e-07	WDR3 first beta-propeller domain
WD40_Gbeta	PF25391	147 - 207	5.3e-08	G protein beta WD-40 repeat protein
Beta-prop_WDR5	PF25175	148 - 209	3.2e-09	WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	149 - 216	7.8e-06	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	174 - 298	4.6e-06	WDR36/Utp21 second beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006319)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G24520 AT5G24520 AT5G24520 AT5G24520
fragaria_vesca	FvH4_7g32970 FvH4_7g32970
malus_domestica	MD01G1228700.v1.1 MD07G1299800.v1.1
prunus_persica	Prupe.2G319500_v2.0.a1 Prupe.2G319500_v2.0.a1 Prupe.2G319500_v2.0.a1
pyrus_communis	pycom01g23670 pycom07g27200
rosa_chinensis	RchiOBHm_Chr1g0382931
rosa_laevigata	RLG00000008438 RLG00000026148
rosa_multiflora	Rmu_co8253715.1_g000001 Rmu_sc0000698.1_g000059 Rmu_sc0018693.1_g000001 Rmu_sc0018693.1_g000002
rosa_roxburghii	Rroxscaffold_4G00277770
rosa_rugosa	Rorug01G0437200
rosa_samantha	Rh1AG462700 Rh1BG419600 Rh1DG451600 Rh5AG038100
rosa_wichuraiana	Rw1G040930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	479, 926
AasI	GACNNNNNNGTC	2 cut(s)	55, 350
AatII	GACGTC	2 cut(s)	300, 413
Acc36I	ACCTGC	3 cut(s)	479, 891, 926
AccBSI	CCGCTC	1 cut(s)	35
AccII	CGCG	1 cut(s)	548
AciI	CCGC	9 cut(s)	35, 68, 109, 242, 325, 404, 776, 933, 941
AcoI	YGGCCR	1 cut(s)	708
AcsI	RAATTY	1 cut(s)	600
AcyI	GRCGYC	2 cut(s)	297, 410
AfaI	GTAC	7 cut(s)	53, 65, 74, 155, 539, 704, 929
AfiI	CCNNNNNNNGG	4 cut(s)	331, 334, 655, 889
AgsI	TTSAA	2 cut(s)	201, 1021
AhdI	GACNNNNNGTC	1 cut(s)	919
AjnI	CCWGG	1 cut(s)	363
AjuI	GAANNNNNNNTTGG	2 cut(s)	242, 274
AluBI	AGCT	8 cut(s)	141, 253, 305, 514, 560, 781, 955, 1015
AluI	AGCT	8 cut(s)	141, 253, 305, 514, 560, 781, 955, 1015
Alw21I	GWGCWC	1 cut(s)	627
Alw26I	GTCTC	1 cut(s)	500
AoxI	GGCC	4 cut(s)	81, 708, 819, 899
ApaI	GGGCCC	1 cut(s)	823
ApeKI	GCWGC	5 cut(s)	138, 511, 955, 968, 1012
ApoI	RAATTY	1 cut(s)	600
ArsI	GACNNNNNNTTYG	2 cut(s)	483, 515
AseI	ATTAAT	1 cut(s)	948
AspLEI	GCGC	2 cut(s)	401, 871
AspS9I	GGNCC	4 cut(s)	819, 820, 880, 899
AsuC2I	CCSGG	3 cut(s)	164, 188, 439
AsuHPI	GGTGA	2 cut(s)	58, 607
AvaII	GGWCC	1 cut(s)	880
BaeGI	GKGCMC	1 cut(s)	823
BalI	TGGCCA	1 cut(s)	710
BanII	GRGCYC	3 cut(s)	438, 650, 823
Bbv12I	GWGCWC	1 cut(s)	627
BbvCI	CCTCAGC	1 cut(s)	651
BbvI	GCAGC	5 cut(s)	150, 523, 942, 955, 1024
BccI	CCATC	3 cut(s)	33, 581, 715
BceAI	ACGGC	2 cut(s)	46, 116
BcgI	CGANNNNNNTGC	2 cut(s)	127, 161
BciT130I	CCWGG	1 cut(s)	365
BciVI	GTATCC	2 cut(s)	184, 652
BcnI	CCSGG	3 cut(s)	164, 188, 439
BcoDI	GTCTC	1 cut(s)	500
BfaI	CTAG	1 cut(s)	561
BfmI	CTRYAG	1 cut(s)	956
BfoI	RGCGCY	1 cut(s)	872
BfuAI	ACCTGC	3 cut(s)	479, 891, 926
BfuI	GTATCC	2 cut(s)	184, 652
BisI	GCNGC	5 cut(s)	139, 512, 956, 969, 1013
BlsI	GCNGC	5 cut(s)	140, 513, 957, 970, 1014
BmcAI	AGTACT	1 cut(s)	155
Bme1390I	CCNGG	4 cut(s)	164, 188, 365, 439
Bme18I	GGWCC	1 cut(s)	880
BmeRI	GACNNNNNGTC	1 cut(s)	919
BmgT120I	GGNCC	4 cut(s)	819, 820, 880, 899
BmiI	GGNNCC	3 cut(s)	821, 822, 881
BmrFI	CCNGG	4 cut(s)	164, 188, 365, 439
BmsI	GCATC	3 cut(s)	468, 756, 999
BplI	GAGNNNNNCTC	2 cut(s)	140, 172
BpmI	CTGGAG	1 cut(s)	803
Bpu10I	CCTNAGC	1 cut(s)	651
BpuMI	CCSGG	3 cut(s)	164, 188, 439
BsaHI	GRCGYC	2 cut(s)	297, 410
BsaJI	CCNNGG	3 cut(s)	78, 437, 888
Bsc4I	CCNNNNNNNGG	4 cut(s)	331, 334, 655, 889
Bse1I	ACTGG	1 cut(s)	770
Bse3DI	GCAATG	1 cut(s)	990
BseBI	CCWGG	1 cut(s)	365
BseDI	CCNNGG	3 cut(s)	78, 437, 888
BseGI	GGATG	1 cut(s)	862
BseLI	CCNNNNNNNGG	4 cut(s)	331, 334, 655, 889
BseMI	GCAATG	1 cut(s)	990
BseMII	CTCAG	3 cut(s)	43, 665, 878
BseNI	ACTGG	1 cut(s)	770
BseRI	GAGGAG	1 cut(s)	164
BseSI	GKGCMC	1 cut(s)	823
BseXI	GCAGC	5 cut(s)	150, 523, 942, 955, 1024
BseYI	CCCAGC	2 cut(s)	851, 896
BsgI	GTGCAG	2 cut(s)	457, 954
Bsh1236I	CGCG	1 cut(s)	548
Bsh1285I	CGRYCG	2 cut(s)	758, 894
BshFI	GGCC	4 cut(s)	83, 710, 821, 901
BsiEI	CGRYCG	2 cut(s)	758, 894
BsiHKAI	GWGCWC	1 cut(s)	627
BsiSI	CCGG	4 cut(s)	163, 188, 439, 656
BsiWI	CGTACG	1 cut(s)	51
BslFI	GGGAC	4 cut(s)	463, 497, 629, 893
BslI	CCNNNNNNNGG	4 cut(s)	331, 334, 655, 889
BsmAI	GTCTC	1 cut(s)	500
BsmBI	CGTCTC	1 cut(s)	500
BsmFI	GGGAC	4 cut(s)	463, 497, 629, 893
BsmI	GAATGC	1 cut(s)	811
BsnI	GGCC	4 cut(s)	83, 710, 821, 901
Bsp120I	GGGCCC	1 cut(s)	819
Bsp1286I	GDGCHC	4 cut(s)	438, 627, 650, 823
Bsp143I	GATC	3 cut(s)	633, 741, 755
Bsp19I	CCATGG	1 cut(s)	78
BspACI	CCGC	9 cut(s)	35, 68, 109, 242, 325, 404, 776, 933, 941
BspANI	GGCC	4 cut(s)	83, 710, 821, 901
BspCNI	CTCAG	3 cut(s)	42, 664, 877
BspFNI	CGCG	1 cut(s)	548
BspLI	GGNNCC	3 cut(s)	821, 822, 881
BspMAI	CTGCAG	1 cut(s)	960
BspMI	ACCTGC	3 cut(s)	479, 891, 926
BsrBI	CCGCTC	1 cut(s)	35
BsrDI	GCAATG	1 cut(s)	990
BsrI	ACTGG	1 cut(s)	770
BssECI	CCNNGG	3 cut(s)	78, 437, 888
BssMI	GATC	3 cut(s)	633, 741, 755
BssNI	GRCGYC	2 cut(s)	297, 410
BssT1I	CCWWGG	1 cut(s)	78
Bst2UI	CCWGG	1 cut(s)	365
Bst4CI	ACNGT	2 cut(s)	352, 892
BstACI	GRCGYC	2 cut(s)	297, 410
BstDEI	CTNAG	4 cut(s)	29, 301, 651, 864
BstDSI	CCRYGG	2 cut(s)	78, 888
BstENI	CCTNNNNNAGG	1 cut(s)	332
BstF5I	GGATG	1 cut(s)	862
BstFNI	CGCG	1 cut(s)	548
BstH2I	RGCGCY	1 cut(s)	872
BstHHI	GCGC	2 cut(s)	401, 871
BstKTI	GATC	3 cut(s)	636, 744, 758
BstMAI	GTCTC	1 cut(s)	500
BstMBI	GATC	3 cut(s)	633, 741, 755
BstMCI	CGRYCG	2 cut(s)	758, 894
BstMWI	GCNNNNNNNGC	6 cut(s)	67, 250, 387, 396, 766, 787
BstNI	CCWGG	1 cut(s)	365
BstSCI	CCNGG	4 cut(s)	162, 186, 363, 437
BstSFI	CTRYAG	1 cut(s)	956
BstSLI	GKGCMC	1 cut(s)	823
BstUI	CGCG	1 cut(s)	548
BstV1I	GCAGC	5 cut(s)	150, 523, 942, 955, 1024
BsuI	GTATCC	2 cut(s)	184, 652
BsuRI	GGCC	4 cut(s)	83, 710, 821, 901
BtgI	CCRYGG	2 cut(s)	78, 888
BtgZI	GCGATG	1 cut(s)	529
BtsCI	GGATG	1 cut(s)	862
BtsI	GCAGTG	1 cut(s)	965
BtsIMutI	CAGTG	2 cut(s)	777, 965
BveI	ACCTGC	3 cut(s)	479, 891, 926
CfoI	GCGC	2 cut(s)	401, 871
Cfr13I	GGNCC	4 cut(s)	819, 820, 880, 899
CseI	GACGC	1 cut(s)	517
Csp6I	GTAC	7 cut(s)	52, 64, 73, 154, 538, 703, 928
CviAII	CATG	4 cut(s)	79, 256, 706, 919
CviQI	GTAC	7 cut(s)	52, 64, 73, 154, 538, 703, 928
DdeI	CTNAG	4 cut(s)	29, 301, 651, 864
DpnI	GATC	3 cut(s)	635, 743, 757
DpnII	GATC	3 cut(s)	633, 741, 755
DrdI	GACNNNNNNGTC	2 cut(s)	55, 350
DriI	GACNNNNNGTC	1 cut(s)	919
DseDI	GACNNNNNNGTC	2 cut(s)	55, 350
EaeI	YGGCCR	1 cut(s)	708
Eam1105I	GACNNNNNGTC	1 cut(s)	919
EciI	GGCGGA	3 cut(s)	231, 314, 791
Eco130I	CCWWGG	1 cut(s)	78
Eco24I	GRGCYC	3 cut(s)	438, 650, 823
Eco32I	GATATC	2 cut(s)	172, 751
Eco47I	GGWCC	1 cut(s)	880
EcoNI	CCTNNNNNAGG	1 cut(s)	332
EcoO109I	RGGNCCY	2 cut(s)	820, 880
EcoRII	CCWGG	1 cut(s)	363
EcoRV	GATATC	2 cut(s)	172, 751
EcoT14I	CCWWGG	1 cut(s)	78
EcoT38I	GRGCYC	3 cut(s)	438, 650, 823
ErhI	CCWWGG	1 cut(s)	78
Esp3I	CGTCTC	1 cut(s)	500
FaeI	CATG	4 cut(s)	82, 259, 709, 922
FaiI	YATR	5 cut(s)	80, 257, 642, 707, 920
FaqI	GGGAC	4 cut(s)	463, 497, 629, 893
FatI	CATG	4 cut(s)	78, 255, 705, 918
FauI	CCCGC	2 cut(s)	75, 116
Fnu4HI	GCNGC	5 cut(s)	139, 512, 956, 969, 1013
FokI	GGATG	1 cut(s)	869
FriOI	GRGCYC	3 cut(s)	438, 650, 823
Fsp4HI	GCNGC	5 cut(s)	139, 512, 956, 969, 1013
FspBI	CTAG	1 cut(s)	561
GlaI	GCGC	2 cut(s)	400, 870
GluI	GCNGC	5 cut(s)	139, 512, 956, 969, 1013
GsaI	CCCAGC	2 cut(s)	855, 900
GsuI	CTGGAG	1 cut(s)	803
HaeII	RGCGCY	1 cut(s)	872
HaeIII	GGCC	4 cut(s)	83, 710, 821, 901
HapII	CCGG	4 cut(s)	163, 188, 439, 656
HgaI	GACGC	1 cut(s)	517
HhaI	GCGC	2 cut(s)	401, 871
Hin1I	GRCGYC	2 cut(s)	297, 410
Hin1II	CATG	4 cut(s)	82, 259, 709, 922
Hin6I	GCGC	2 cut(s)	399, 869
HinP1I	GCGC	2 cut(s)	399, 869
HinfI	GANTC	5 cut(s)	20, 56, 126, 344, 909
HpaII	CCGG	4 cut(s)	163, 188, 439, 656
HphI	GGTGA	2 cut(s)	58, 607
Hpy166II	GTNNAC	1 cut(s)	538
Hpy188I	TCNGA	2 cut(s)	32, 755
Hpy188III	TCNNGA	2 cut(s)	505, 524
Hpy8I	GTNNAC	1 cut(s)	538
Hpy99I	CGWCG	5 cut(s)	137, 299, 356, 506, 766
HpyAV	CCTTC	3 cut(s)	190, 235, 1015
HpyCH4III	ACNGT	2 cut(s)	352, 892
HpyCH4IV	ACGT	3 cut(s)	50, 297, 410
HpyCH4V	TGCA	5 cut(s)	474, 958, 971, 995, 1012
HpyF10VI	GCNNNNNNNGC	6 cut(s)	67, 250, 387, 396, 766, 787
HpyF3I	CTNAG	4 cut(s)	29, 301, 651, 864
HpySE526I	ACGT	3 cut(s)	50, 297, 410
Hsp92I	GRCGYC	2 cut(s)	297, 410
Hsp92II	CATG	4 cut(s)	82, 259, 709, 922
HspAI	GCGC	2 cut(s)	399, 869
Kzo9I	GATC	3 cut(s)	633, 741, 755
LmnI	GCTCC	3 cut(s)	406, 622, 778
Lsp1109I	GCAGC	5 cut(s)	150, 523, 942, 955, 1024
LweI	GCATC	3 cut(s)	468, 756, 999
MaeI	CTAG	1 cut(s)	561
MaeII	ACGT	3 cut(s)	50, 297, 410
MaeIII	GTNAC	1 cut(s)	46
MalI	GATC	3 cut(s)	635, 743, 757
MbiI	CCGCTC	1 cut(s)	35
MboI	GATC	3 cut(s)	633, 741, 755
MhlI	GDGCHC	4 cut(s)	438, 627, 650, 823
MlsI	TGGCCA	1 cut(s)	710
MluCI	AATT	4 cut(s)	444, 477, 600, 714
MluNI	TGGCCA	1 cut(s)	710
MlyI	GAGTC	2 cut(s)	65, 338
Mox20I	TGGCCA	1 cut(s)	710
MscI	TGGCCA	1 cut(s)	710
MseI	TTAA	2 cut(s)	371, 948
MslI	CAYNNNNRTG	1 cut(s)	923
Msp20I	TGGCCA	1 cut(s)	710
MspA1I	CMGCKG	4 cut(s)	70, 406, 514, 955
MspI	CCGG	4 cut(s)	163, 188, 439, 656
MspR9I	CCNGG	4 cut(s)	164, 188, 365, 439
Mva1269I	GAATGC	1 cut(s)	811
MvaI	CCWGG	1 cut(s)	365
MvnI	CGCG	1 cut(s)	548
MwoI	GCNNNNNNNGC	6 cut(s)	67, 250, 387, 396, 766, 787
NciI	CCSGG	3 cut(s)	164, 188, 439
NcoI	CCATGG	1 cut(s)	78
NdeII	GATC	3 cut(s)	633, 741, 755
NlaIII	CATG	4 cut(s)	82, 259, 709, 922
NlaIV	GGNNCC	3 cut(s)	821, 822, 881
NmuCI	GTSAC	1 cut(s)	46
PaqCI	CACCTGC	2 cut(s)	479, 926
PcsI	WCGNNNNNNNCGW	1 cut(s)	423
PctI	GAATGC	1 cut(s)	811
PfeI	GAWTC	3 cut(s)	20, 126, 909
Pfl23II	CGTACG	1 cut(s)	51
PkrI	GCNGC	5 cut(s)	140, 513, 957, 970, 1014
Ple19I	CGATCG	1 cut(s)	758
PleI	GAGTC	2 cut(s)	64, 338
PpsI	GAGTC	2 cut(s)	64, 338
PpuMI	RGGWCCY	1 cut(s)	880
PshBI	ATTAAT	1 cut(s)	948
Psp5II	RGGWCCY	1 cut(s)	880
Psp6I	CCWGG	1 cut(s)	363
PspFI	CCCAGC	2 cut(s)	851, 896
PspGI	CCWGG	1 cut(s)	363
PspLI	CGTACG	1 cut(s)	51
PspN4I	GGNNCC	3 cut(s)	821, 822, 881
PspOMI	GGGCCC	1 cut(s)	819
PspPI	GGNCC	4 cut(s)	819, 820, 880, 899
PspPPI	RGGWCCY	1 cut(s)	880
PstI	CTGCAG	1 cut(s)	960
PvuI	CGATCG	1 cut(s)	758
PvuII	CAGCTG	2 cut(s)	514, 955
RsaI	GTAC	7 cut(s)	53, 65, 74, 155, 539, 704, 929
RsaNI	GTAC	7 cut(s)	52, 64, 73, 154, 538, 703, 928
RseI	CAYNNNNRTG	1 cut(s)	923
SaqAI	TTAA	2 cut(s)	371, 948
SatI	GCNGC	5 cut(s)	139, 512, 956, 969, 1013
Sau3AI	GATC	3 cut(s)	633, 741, 755
Sau96I	GGNCC	4 cut(s)	819, 820, 880, 899
ScaI	AGTACT	1 cut(s)	155
SchI	GAGTC	2 cut(s)	65, 338
ScrFI	CCNGG	4 cut(s)	164, 188, 365, 439
SduI	GDGCHC	4 cut(s)	438, 627, 650, 823
SfaNI	GCATC	3 cut(s)	468, 756, 999
SfcI	CTRYAG	1 cut(s)	956
SinI	GGWCC	1 cut(s)	880
SmiMI	CAYNNNNRTG	1 cut(s)	923
Sse9I	AATT	4 cut(s)	444, 477, 600, 714
SsiI	CCGC	9 cut(s)	35, 68, 109, 242, 325, 404, 776, 933, 941
SspMI	CTAG	1 cut(s)	561
StyD4I	CCNGG	4 cut(s)	162, 186, 363, 437
StyI	CCWWGG	1 cut(s)	78
TaaI	ACNGT	2 cut(s)	352, 892
TaiI	ACGT	3 cut(s)	53, 300, 413
TasI	AATT	4 cut(s)	444, 477, 600, 714
TatI	WGTACW	2 cut(s)	153, 927
TfiI	GAWTC	3 cut(s)	20, 126, 909
Tru1I	TTAA	2 cut(s)	371, 948
Tru9I	TTAA	2 cut(s)	371, 948
TscAI	CASTG	2 cut(s)	777, 965
TseFI	GTSAC	1 cut(s)	46
TseI	GCWGC	5 cut(s)	138, 511, 955, 968, 1012
Tsp45I	GTSAC	1 cut(s)	46
TspRI	CASTG	2 cut(s)	777, 965
VpaK11BI	GGWCC	1 cut(s)	880
VspI	ATTAAT	1 cut(s)	948
XagI	CCTNNNNNAGG	1 cut(s)	332
XapI	RAATTY	1 cut(s)	600
XcmI	CCANNNNNNNNNTGG	1 cut(s)	718
XspI	CTAG	1 cut(s)	561
ZraI	GACGTC	2 cut(s)	298, 411
ZrmI	AGTACT	1 cut(s)	155

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.