MD07G1299800.v1.1

Protein TRANSPARENT TESTA GLABRA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006319
Physical Location & Seq
Reverse (-)
35867726 .. 35870601
2876 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1299800.v1.1.491

Sequence Viewer

Length: 1029 bp
ATGGAGAACTCTACGCAAGAATCGCATCTCCGACAGGAGAACTCGGTGACGTACGAGTCGCCGTACCCGCTGTACGCCATGGCCTTCCCCTCGCCCCAAACCCGAACCCGCCACCAGCACCATCGGATCGCCGTCGGCAGCTTTATCGAGGAGTACTCGAACCGGGTCGACATCCTCTCCTTCGACCCGGATACCCTTTCAATAAAACTGAACCCGACCCTCTCCTTCGACCACCCTTATCCGCCCACCAAGCTCATGTTCCACCCGAACCCCAACGCCCTCCATAAAACCAATGACCTCTTAGCTTCCTCCGGCGACTACCTCCGCCTCTGGGAGGTCGGCGACTCCTCCGTCGAGCCCATCCAGGTTTTAAACAACAGCAAGACCAGCGAGTTCTGCGCCCCACTGACGTCGTTCGATTGGAACGACATCGAGCCCCGGCGAATTGGGACTTCCAGCATCGACACCACCTGCACAATTTGGGACATTGAAAAGGGGGTCGTCGAAACCCAGCTGATTGCGCACGATAAAGAGGTATACGATATCGCGTGGGGGGAAGCTAGGGTTTTTGCTTCGGTTTCGGCTGATGGGTCGGTGAGAATTTTCGATTTGAGGGACAAGGAGCACTCCACGATCATCTACGAGAGCCCTCAGCCGGATACCCCTTTGCTCCGATTGGCTTGGAACAAGCAGGATTTGAGGTCCATGGCTACAATTCTGATGGACAGCAATAAAGTTGTGATCTTGGATATCCGATCGCCGACCATGCCAGTGGCGGAGCTGGAGAGGCACAGAGGTAGTGTAAATGCTATTGCTTGGGCCCCCCAGAGTTGTAGGCACATTTGCTCTGCTGGGGATGACACTCGGGCGCTTATTTGGGACCTGCCCACGGTTGCTGGGCCGAATGGAATCGACCCCATGTCGATGTACTCCGCAGGTGCGGAGATTAATCAGCTGCAGTGGTCTGCTGCACAGCCTGATTGGATTTCCATTGCATTTTCTAACAAGATGCAGCTTTTGAAGGTGTGA

Protein Analysis

343

Amino Acids

38.52

Weight (kDa)

5.12

Isoelectric Point (pI)

52.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EIPR1 PF23609 33 - 291 1.3e-07 EIPR1 beta-propeller
WD40_CDC20-Fz PF24807 96 - 329 1.1e-18 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 134 - 336 1.3e-09 WDHD1 first WD40 domain
WD40_Prp19 PF24814 137 - 213 8.4e-08 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 138 - 215 4.8e-11 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 146 - 210 8.9e-07 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 147 - 207 5.3e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 148 - 209 3e-09 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 149 - 215 8.4e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 174 - 298 1.7e-06 WDR36/Utp21 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 479, 926
AasI GACNNNNNNGTC 2 cut(s) 55, 350
AatII GACGTC 1 cut(s) 413
Acc16I TGCGCA 1 cut(s) 522
Acc36I ACCTGC 3 cut(s) 479, 891, 926
AccI GTMKAC 2 cut(s) 168, 537
AccII CGCG 1 cut(s) 548
AciI CCGC 7 cut(s) 68, 109, 242, 325, 776, 933, 941
AclWI GGATC 1 cut(s) 134
AcsI RAATTY 1 cut(s) 600
AcyI GRCGYC 1 cut(s) 410
AfaI GTAC 5 cut(s) 53, 65, 74, 155, 929
AfiI CCNNNNNNNGG 4 cut(s) 331, 334, 655, 889
AgsI TTSAA 3 cut(s) 201, 491, 1021
AhdI GACNNNNNGTC 1 cut(s) 919
AjnI CCWGG 1 cut(s) 363
AjuI GAANNNNNNNTTGG 2 cut(s) 242, 274
AluBI AGCT 8 cut(s) 141, 253, 305, 514, 560, 781, 955, 1015
AluI AGCT 8 cut(s) 141, 253, 305, 514, 560, 781, 955, 1015
Alw21I GWGCWC 1 cut(s) 627
AlwI GGATC 1 cut(s) 134
Ama87I CYCGRG 1 cut(s) 864
AoxI GGCC 3 cut(s) 81, 819, 899
ApaI GGGCCC 1 cut(s) 823
ApeKI GCWGC 4 cut(s) 138, 955, 968, 1012
ApoI RAATTY 1 cut(s) 600
AseI ATTAAT 1 cut(s) 948
AspLEI GCGC 3 cut(s) 401, 523, 871
AspS9I GGNCC 5 cut(s) 702, 819, 820, 880, 899
AsuC2I CCSGG 3 cut(s) 164, 188, 439
AsuHPI GGTGA 2 cut(s) 58, 607
AvaI CYCGRG 1 cut(s) 864
AvaII GGWCC 2 cut(s) 702, 880
BaeGI GKGCMC 1 cut(s) 823
BanII GRGCYC 4 cut(s) 360, 438, 650, 823
Bbv12I GWGCWC 1 cut(s) 627
BbvCI CCTCAGC 1 cut(s) 651
BbvI GCAGC 4 cut(s) 150, 942, 955, 1024
BccI CCATC 4 cut(s) 129, 368, 581, 715
BceAI ACGGC 2 cut(s) 46, 116
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 1 cut(s) 365
BciVI GTATCC 2 cut(s) 184, 652
BcnI CCSGG 3 cut(s) 164, 188, 439
BfaI CTAG 1 cut(s) 561
BfmI CTRYAG 1 cut(s) 956
BfoI RGCGCY 1 cut(s) 872
BfuAI ACCTGC 3 cut(s) 479, 891, 926
BfuI GTATCC 2 cut(s) 184, 652
BisI GCNGC 4 cut(s) 139, 956, 969, 1013
BlsI GCNGC 4 cut(s) 140, 957, 970, 1014
BmcAI AGTACT 1 cut(s) 155
Bme1390I CCNGG 4 cut(s) 164, 188, 365, 439
Bme18I GGWCC 2 cut(s) 702, 880
BmeRI GACNNNNNGTC 1 cut(s) 919
BmeT110I CYCGRG 1 cut(s) 864
BmgT120I GGNCC 5 cut(s) 702, 819, 820, 880, 899
BmiI GGNNCC 3 cut(s) 821, 822, 881
BmrFI CCNGG 4 cut(s) 164, 188, 365, 439
BmsI GCATC 3 cut(s) 34, 468, 999
BplI GAGNNNNNCTC 2 cut(s) 140, 172
BpmI CTGGAG 1 cut(s) 803
Bpu10I CCTNAGC 1 cut(s) 651
BpuMI CCSGG 3 cut(s) 164, 188, 439
BsaHI GRCGYC 1 cut(s) 410
BsaJI CCNNGG 4 cut(s) 78, 437, 705, 888
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 4 cut(s) 331, 334, 655, 889
Bse1I ACTGG 1 cut(s) 770
Bse3DI GCAATG 1 cut(s) 990
BseBI CCWGG 1 cut(s) 365
BseDI CCNNGG 4 cut(s) 78, 437, 705, 888
BseGI GGATG 3 cut(s) 171, 360, 862
BseLI CCNNNNNNNGG 4 cut(s) 331, 334, 655, 889
BseMI GCAATG 1 cut(s) 990
BseMII CTCAG 1 cut(s) 665
BseNI ACTGG 1 cut(s) 770
BseRI GAGGAG 2 cut(s) 164, 337
BseSI GKGCMC 1 cut(s) 823
BseXI GCAGC 4 cut(s) 150, 942, 955, 1024
BseYI CCCAGC 3 cut(s) 510, 851, 896
BsgI GTGCAG 2 cut(s) 457, 954
Bsh1236I CGCG 1 cut(s) 548
Bsh1285I CGRYCG 1 cut(s) 758
BshFI GGCC 3 cut(s) 83, 821, 901
BsiEI CGRYCG 1 cut(s) 758
BsiHKAI GWGCWC 1 cut(s) 627
BsiHKCI CYCGRG 1 cut(s) 864
BsiSI CCGG 5 cut(s) 163, 188, 312, 439, 656
BsiWI CGTACG 1 cut(s) 51
BslFI GGGAC 4 cut(s) 463, 497, 629, 893
BslI CCNNNNNNNGG 4 cut(s) 331, 334, 655, 889
BsmFI GGGAC 4 cut(s) 463, 497, 629, 893
BsnI GGCC 3 cut(s) 83, 821, 901
BsoBI CYCGRG 1 cut(s) 864
Bsp120I GGGCCC 1 cut(s) 819
Bsp1286I GDGCHC 5 cut(s) 360, 438, 627, 650, 823
Bsp143I GATC 4 cut(s) 126, 633, 741, 755
Bsp19I CCATGG 2 cut(s) 78, 705
BspACI CCGC 7 cut(s) 68, 109, 242, 325, 776, 933, 941
BspANI GGCC 3 cut(s) 83, 821, 901
BspCNI CTCAG 1 cut(s) 664
BspFNI CGCG 1 cut(s) 548
BspLI GGNNCC 3 cut(s) 821, 822, 881
BspMAI CTGCAG 1 cut(s) 960
BspMI ACCTGC 3 cut(s) 479, 891, 926
BspPI GGATC 1 cut(s) 134
BsrDI GCAATG 1 cut(s) 990
BsrI ACTGG 1 cut(s) 770
BssECI CCNNGG 4 cut(s) 78, 437, 705, 888
BssMI GATC 4 cut(s) 126, 633, 741, 755
BssNAI GTATAC 1 cut(s) 538
BssNI GRCGYC 1 cut(s) 410
BssT1I CCWWGG 2 cut(s) 78, 705
Bst1107I GTATAC 1 cut(s) 538
Bst2UI CCWGG 1 cut(s) 365
Bst4CI ACNGT 1 cut(s) 892
BstACI GRCGYC 1 cut(s) 410
BstDEI CTNAG 2 cut(s) 301, 651
BstDSI CCRYGG 3 cut(s) 78, 705, 888
BstENI CCTNNNNNAGG 1 cut(s) 332
BstF5I GGATG 3 cut(s) 171, 360, 862
BstFNI CGCG 1 cut(s) 548
BstH2I RGCGCY 1 cut(s) 872
BstHHI GCGC 3 cut(s) 401, 523, 871
BstKTI GATC 4 cut(s) 129, 636, 744, 758
BstMBI GATC 4 cut(s) 126, 633, 741, 755
BstMCI CGRYCG 1 cut(s) 758
BstMWI GCNNNNNNNGC 8 cut(s) 22, 67, 250, 387, 396, 520, 766, 787
BstNI CCWGG 1 cut(s) 365
BstSCI CCNGG 4 cut(s) 162, 186, 363, 437
BstSFI CTRYAG 1 cut(s) 956
BstSLI GKGCMC 1 cut(s) 823
BstUI CGCG 1 cut(s) 548
BstV1I GCAGC 4 cut(s) 150, 942, 955, 1024
BstXI CCANNNNNNTGG 1 cut(s) 772
BstZ17I GTATAC 1 cut(s) 538
BsuI GTATCC 2 cut(s) 184, 652
BsuRI GGCC 3 cut(s) 83, 821, 901
BtgI CCRYGG 3 cut(s) 78, 705, 888
BtsCI GGATG 3 cut(s) 171, 360, 862
BtsI GCAGTG 1 cut(s) 965
BtsIMutI CAGTG 3 cut(s) 404, 777, 965
BveI ACCTGC 3 cut(s) 479, 891, 926
CfoI GCGC 3 cut(s) 401, 523, 871
Cfr13I GGNCC 5 cut(s) 702, 819, 820, 880, 899
Csp6I GTAC 5 cut(s) 52, 64, 73, 154, 928
CviAII CATG 5 cut(s) 79, 256, 706, 766, 919
CviQI GTAC 5 cut(s) 52, 64, 73, 154, 928
DdeI CTNAG 2 cut(s) 301, 651
DpnI GATC 4 cut(s) 128, 635, 743, 757
DpnII GATC 4 cut(s) 126, 633, 741, 755
DraI TTTAAA 1 cut(s) 372
DrdI GACNNNNNNGTC 2 cut(s) 55, 350
DriI GACNNNNNGTC 1 cut(s) 919
DseDI GACNNNNNNGTC 2 cut(s) 55, 350
Eam1105I GACNNNNNGTC 1 cut(s) 919
EciI GGCGGA 3 cut(s) 231, 314, 791
Eco130I CCWWGG 2 cut(s) 78, 705
Eco24I GRGCYC 4 cut(s) 360, 438, 650, 823
Eco32I GATATC 2 cut(s) 544, 751
Eco47I GGWCC 2 cut(s) 702, 880
Eco88I CYCGRG 1 cut(s) 864
EcoNI CCTNNNNNAGG 1 cut(s) 332
EcoO109I RGGNCCY 2 cut(s) 820, 880
EcoRII CCWGG 1 cut(s) 363
EcoRV GATATC 2 cut(s) 544, 751
EcoT14I CCWWGG 2 cut(s) 78, 705
EcoT38I GRGCYC 4 cut(s) 360, 438, 650, 823
ErhI CCWWGG 2 cut(s) 78, 705
FaeI CATG 5 cut(s) 82, 259, 709, 769, 922
FaiI YATR 7 cut(s) 80, 257, 285, 538, 707, 767, 920
FaqI GGGAC 4 cut(s) 463, 497, 629, 893
FatI CATG 5 cut(s) 78, 255, 705, 765, 918
FauI CCCGC 2 cut(s) 75, 116
FblI GTMKAC 2 cut(s) 168, 537
Fnu4HI GCNGC 4 cut(s) 139, 956, 969, 1013
FokI GGATG 3 cut(s) 158, 347, 869
FriOI GRGCYC 4 cut(s) 360, 438, 650, 823
Fsp4HI GCNGC 4 cut(s) 139, 956, 969, 1013
FspBI CTAG 1 cut(s) 561
FspI TGCGCA 1 cut(s) 522
GlaI GCGC 3 cut(s) 400, 522, 870
GluI GCNGC 4 cut(s) 139, 956, 969, 1013
GsaI CCCAGC 3 cut(s) 514, 855, 900
GsuI CTGGAG 1 cut(s) 803
HaeII RGCGCY 1 cut(s) 872
HaeIII GGCC 3 cut(s) 83, 821, 901
HapII CCGG 5 cut(s) 163, 188, 312, 439, 656
HhaI GCGC 3 cut(s) 401, 523, 871
Hin1I GRCGYC 1 cut(s) 410
Hin1II CATG 5 cut(s) 82, 259, 709, 769, 922
Hin6I GCGC 3 cut(s) 399, 521, 869
HinP1I GCGC 3 cut(s) 399, 521, 869
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HinfI GANTC 4 cut(s) 20, 56, 344, 909
HpaII CCGG 5 cut(s) 163, 188, 312, 439, 656
HphI GGTGA 2 cut(s) 58, 607
Hpy166II GTNNAC 2 cut(s) 169, 538
Hpy188I TCNGA 5 cut(s) 32, 126, 674, 720, 755
Hpy8I GTNNAC 2 cut(s) 169, 538
Hpy99I CGWCG 4 cut(s) 137, 356, 415, 506
HpyAV CCTTC 4 cut(s) 94, 190, 235, 1015
HpyCH4III ACNGT 1 cut(s) 892
HpyCH4IV ACGT 2 cut(s) 50, 410
HpyCH4V TGCA 5 cut(s) 474, 958, 971, 995, 1012
HpyF10VI GCNNNNNNNGC 8 cut(s) 22, 67, 250, 387, 396, 520, 766, 787
HpyF3I CTNAG 2 cut(s) 301, 651
HpySE526I ACGT 2 cut(s) 50, 410
Hsp92I GRCGYC 1 cut(s) 410
Hsp92II CATG 5 cut(s) 82, 259, 709, 769, 922
HspAI GCGC 3 cut(s) 399, 521, 869
Kzo9I GATC 4 cut(s) 126, 633, 741, 755
LmnI GCTCC 3 cut(s) 622, 675, 778
Lsp1109I GCAGC 4 cut(s) 150, 942, 955, 1024
LweI GCATC 3 cut(s) 34, 468, 999
MaeI CTAG 1 cut(s) 561
MaeII ACGT 2 cut(s) 50, 410
MaeIII GTNAC 1 cut(s) 46
MalI GATC 4 cut(s) 128, 635, 743, 757
MboI GATC 4 cut(s) 126, 633, 741, 755
MhlI GDGCHC 5 cut(s) 360, 438, 627, 650, 823
MluCI AATT 4 cut(s) 444, 477, 600, 714
MlyI GAGTC 2 cut(s) 65, 338
MmeI TCCRAC 1 cut(s) 55
MseI TTAA 2 cut(s) 371, 948
MslI CAYNNNNRTG 2 cut(s) 770, 923
MspA1I CMGCKG 3 cut(s) 70, 514, 955
MspI CCGG 5 cut(s) 163, 188, 312, 439, 656
MspR9I CCNGG 4 cut(s) 164, 188, 365, 439
MvaI CCWGG 1 cut(s) 365
MvnI CGCG 1 cut(s) 548
MwoI GCNNNNNNNGC 8 cut(s) 22, 67, 250, 387, 396, 520, 766, 787
NciI CCSGG 3 cut(s) 164, 188, 439
NcoI CCATGG 2 cut(s) 78, 705
NdeII GATC 4 cut(s) 126, 633, 741, 755
NlaIII CATG 5 cut(s) 82, 259, 709, 769, 922
NlaIV GGNNCC 3 cut(s) 821, 822, 881
NmuCI GTSAC 1 cut(s) 46
NsbI TGCGCA 1 cut(s) 522
PaqCI CACCTGC 2 cut(s) 479, 926
PcsI WCGNNNNNNNCGW 1 cut(s) 423
PfeI GAWTC 2 cut(s) 20, 909
Pfl23II CGTACG 1 cut(s) 51
PkrI GCNGC 4 cut(s) 140, 957, 970, 1014
Ple19I CGATCG 1 cut(s) 758
PleI GAGTC 2 cut(s) 64, 338
PpsI GAGTC 2 cut(s) 64, 338
PpuMI RGGWCCY 1 cut(s) 880
PshBI ATTAAT 1 cut(s) 948
Psp5II RGGWCCY 1 cut(s) 880
Psp6I CCWGG 1 cut(s) 363
PspFI CCCAGC 3 cut(s) 510, 851, 896
PspGI CCWGG 1 cut(s) 363
PspLI CGTACG 1 cut(s) 51
PspN4I GGNNCC 3 cut(s) 821, 822, 881
PspOMI GGGCCC 1 cut(s) 819
PspPI GGNCC 5 cut(s) 702, 819, 820, 880, 899
PspPPI RGGWCCY 1 cut(s) 880
PstI CTGCAG 1 cut(s) 960
PvuI CGATCG 1 cut(s) 758
PvuII CAGCTG 2 cut(s) 514, 955
RsaI GTAC 5 cut(s) 53, 65, 74, 155, 929
RsaNI GTAC 5 cut(s) 52, 64, 73, 154, 928
RseI CAYNNNNRTG 2 cut(s) 770, 923
SalI GTCGAC 1 cut(s) 167
SaqAI TTAA 2 cut(s) 371, 948
SatI GCNGC 4 cut(s) 139, 956, 969, 1013
Sau3AI GATC 4 cut(s) 126, 633, 741, 755
Sau96I GGNCC 5 cut(s) 702, 819, 820, 880, 899
ScaI AGTACT 1 cut(s) 155
SchI GAGTC 2 cut(s) 65, 338
ScrFI CCNGG 4 cut(s) 164, 188, 365, 439
SduI GDGCHC 5 cut(s) 360, 438, 627, 650, 823
SfaNI GCATC 3 cut(s) 34, 468, 999
SfcI CTRYAG 1 cut(s) 956
SinI GGWCC 2 cut(s) 702, 880
SmiMI CAYNNNNRTG 2 cut(s) 770, 923
Sse9I AATT 4 cut(s) 444, 477, 600, 714
SsiI CCGC 7 cut(s) 68, 109, 242, 325, 776, 933, 941
SspMI CTAG 1 cut(s) 561
StyD4I CCNGG 4 cut(s) 162, 186, 363, 437
StyI CCWWGG 2 cut(s) 78, 705
TaaI ACNGT 1 cut(s) 892
TaiI ACGT 2 cut(s) 53, 413
TasI AATT 4 cut(s) 444, 477, 600, 714
TatI WGTACW 2 cut(s) 153, 927
TfiI GAWTC 2 cut(s) 20, 909
Tru1I TTAA 2 cut(s) 371, 948
Tru9I TTAA 2 cut(s) 371, 948
TscAI CASTG 3 cut(s) 411, 777, 965
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 4 cut(s) 138, 955, 968, 1012
Tsp45I GTSAC 1 cut(s) 46
TspGWI ACGGA 1 cut(s) 340
TspRI CASTG 3 cut(s) 411, 777, 965
VpaK11BI GGWCC 2 cut(s) 702, 880
VspI ATTAAT 1 cut(s) 948
XagI CCTNNNNNAGG 1 cut(s) 332
XapI RAATTY 1 cut(s) 600
XmiI GTMKAC 2 cut(s) 168, 537
XspI CTAG 1 cut(s) 561
ZraI GACGTC 1 cut(s) 411
ZrmI AGTACT 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.