RLG00000026148

Protein TRANSPARENT TESTA GLABRA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0006319
Physical Location & Seq
Forward (+)
840307 .. 841347
1041 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026148

Sequence Viewer

Length: 1041 bp
ATGGAGAACTCGACCCAAGAATCCCACCTCGGCCCCGAAAACTCCGTCACCTACGACTCCCCCTACCCTCTCTACGCCATGGCGTTTTCCCCGGCCGCCCCGACCCGAACCCGCCACAACCACCCCCGCATCGCCGTCGGCAGCTTCATCGAGGAGTTCTCCAACCGGGTCGACATTCTCTCCTTCGACCCGGACACCCTAACCCTAAAGCCCAACCCTTCCCTCTCCTTCGACCACCCCTACCCTCCCACCAAGCTCATGTTCCACCCAAACCCTAACTCCCTCCACAAGTCCAACGACATCCTCGCCTCCTCCGGCGACTACCTCCGCCTCTGGGAGGTCAAAGACTCCTCCGTCGACCGCCTGGAGCCCATCTCCGTCCTCAACAACTCCAAGACCAGCGAGTTCTGCGCGCCGCTGACGTCATTCGACTGGAACGAGATCGAGCCTCGGCGAATCGGAACCTCCAGCATCGACACCACCTGTACGATCTGGGACATCGAGAAGGGCGTCGTCGAGACGCAGCTGATCGCTCACGATAAGGAGGTTTACGACATTGCTTGGGGCGAAGCTAGGGTTTTCGCCTCCGTCTCCGCCGACGGGTCGGTGAGGATTTTTGATCTGAGGGATAAGGAGCATTCCACGATTATATACGAGAGTCCTCAGCCGGACACGCCTCTGCTCCGGCTGGCCTGGAACAAGCAGGACCTGAGGTACATGGCGACGATTCTGATGGATAGTAACAAGGTGGTGATATTGGATATTCGATCGCCGACGATGCCGGTGGCGGAGCTGGAGAGGCACAGGGGGAGTGTGAATGCCATTGCGTGGGCGCCGCAGAGTGCTAGGCATATTTGTTCGGCGGGGGATGACTCGCAGGCGCTTATTTGGGAGCTGCCGACCGTGGCGGGGCCGAATGGGATTGATCCAATGTCAATGTACTCGGCCGGTGCGGAGATTAATCAGCTCCAGTGGTCTGCCGCGCAGCCGGATTGGATTGCTATTGCGTTTTCTAATAAGATGCAGCTTTTGAAGGTTTGA

Protein Analysis

347

Amino Acids

38.74

Weight (kDa)

5.0

Isoelectric Point (pI)

54.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EIPR1 PF23609 34 - 297 1.8e-07 EIPR1 beta-propeller
Beta-prop_TEP1_2nd PF25047 94 - 212 4.9e-06 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 96 - 329 2.8e-19 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 136 - 339 1.8e-10 WDHD1 first WD40 domain
WD40_Prp19 PF24814 141 - 217 7.4e-08 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 142 - 301 1.3e-16 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 151 - 214 1.4e-06 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 151 - 211 5.6e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 152 - 213 3.6e-09 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 152 - 220 7.9e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 164 - 301 5.9e-09 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 353
AatII GACGTC 1 cut(s) 425
AccB1I GGYRCC 1 cut(s) 832
AccB7I CCANNNNNTGG 1 cut(s) 828
AccI GTMKAC 2 cut(s) 171, 357
AccII CGCG 2 cut(s) 413, 983
AclWI GGATC 1 cut(s) 920
AcoI YGGCCR 2 cut(s) 93, 945
AcyI GRCGYC 3 cut(s) 422, 510, 833
AfaI GTAC 3 cut(s) 487, 716, 941
AfiI CCNNNNNNNGG 5 cut(s) 334, 337, 600, 828, 909
AgsI TTSAA 1 cut(s) 1033
AjnI CCWGG 2 cut(s) 363, 692
AjuI GAANNNNNNNTTGG 2 cut(s) 245, 277
AluBI AGCT 8 cut(s) 144, 256, 526, 572, 793, 895, 967, 1027
AluI AGCT 8 cut(s) 144, 256, 526, 572, 793, 895, 967, 1027
Alw26I GTCTC 2 cut(s) 512, 595
AlwI GGATC 1 cut(s) 920
AlwNI CAGNNNCTG 1 cut(s) 709
AoxI GGCC 5 cut(s) 31, 93, 690, 911, 945
ApeKI GCWGC 5 cut(s) 141, 523, 895, 985, 1024
ArsI GACNNNNNNTTYG 2 cut(s) 30, 62
AseI ATTAAT 1 cut(s) 960
AspLEI GCGC 5 cut(s) 413, 415, 835, 883, 985
AspS9I GGNCC 3 cut(s) 32, 706, 911
AsuC2I CCSGG 3 cut(s) 92, 167, 191
AsuHPI GGTGA 3 cut(s) 40, 619, 763
AvaII GGWCC 1 cut(s) 706
AxyI CCTNAGG 1 cut(s) 710
BanI GGYRCC 1 cut(s) 832
BanII GRGCYC 1 cut(s) 372
BbvCI CCTCAGC 1 cut(s) 663
BbvI GCAGC 5 cut(s) 153, 535, 882, 997, 1036
BccI CCATC 2 cut(s) 380, 727
BceAI ACGGC 1 cut(s) 119
BcgI CGANNNNNNTGC 4 cut(s) 118, 130, 152, 164
BciT130I CCWGG 2 cut(s) 365, 694
BcnI CCSGG 3 cut(s) 92, 167, 191
BcoDI GTCTC 2 cut(s) 512, 595
BfaI CTAG 2 cut(s) 573, 846
BfoI RGCGCY 2 cut(s) 836, 884
BisI GCNGC 9 cut(s) 96, 142, 416, 524, 836, 896, 981, 986, 1025
BlsI GCNGC 9 cut(s) 97, 143, 417, 525, 837, 897, 982, 987, 1026
Bme1390I CCNGG 5 cut(s) 92, 167, 191, 365, 694
Bme18I GGWCC 1 cut(s) 706
BmgT120I GGNCC 3 cut(s) 32, 706, 911
BmiI GGNNCC 5 cut(s) 34, 369, 463, 834, 912
BmrFI CCNGG 5 cut(s) 92, 167, 191, 365, 694
BmsI GCATC 4 cut(s) 138, 480, 768, 1011
BplI GAGNNNNNCTC 4 cut(s) 143, 175, 359, 391
BpmI CTGGAG 4 cut(s) 386, 451, 815, 953
Bpu10I CCTNAGC 1 cut(s) 663
BpuMI CCSGG 3 cut(s) 92, 167, 191
BsaHI GRCGYC 3 cut(s) 422, 510, 833
BsaJI CCNNGG 5 cut(s) 28, 78, 90, 449, 903
BsaXI ACNNNNNCTCC 4 cut(s) 164, 194, 263, 293
Bsc4I CCNNNNNNNGG 5 cut(s) 334, 337, 600, 828, 909
Bse118I RCCGGY 2 cut(s) 781, 947
Bse1I ACTGG 2 cut(s) 437, 970
Bse21I CCTNAGG 1 cut(s) 710
Bse3DI GCAATG 2 cut(s) 555, 822
BseBI CCWGG 2 cut(s) 365, 694
BseDI CCNNGG 5 cut(s) 28, 78, 90, 449, 903
BseGI GGATG 2 cut(s) 300, 874
BseLI CCNNNNNNNGG 5 cut(s) 334, 337, 600, 828, 909
BseMI GCAATG 2 cut(s) 555, 822
BseMII CTCAG 3 cut(s) 614, 677, 701
BseNI ACTGG 2 cut(s) 437, 970
BsePI GCGCGC 1 cut(s) 411
BseRI GAGGAG 3 cut(s) 167, 301, 340
BseX3I CGGCCG 2 cut(s) 93, 945
BseXI GCAGC 5 cut(s) 153, 535, 882, 997, 1036
Bsh1236I CGCG 2 cut(s) 413, 983
Bsh1285I CGRYCG 5 cut(s) 96, 361, 770, 903, 948
BshFI GGCC 5 cut(s) 33, 95, 692, 913, 947
BshNI GGYRCC 1 cut(s) 832
BsiEI CGRYCG 5 cut(s) 96, 361, 770, 903, 948
BsiSI CCGG 9 cut(s) 92, 166, 191, 315, 668, 685, 782, 948, 989
BslFI GGGAC 1 cut(s) 509
BslI CCNNNNNNNGG 5 cut(s) 334, 337, 600, 828, 909
BsmAI GTCTC 2 cut(s) 512, 595
BsmBI CGTCTC 2 cut(s) 512, 595
BsmFI GGGAC 1 cut(s) 509
BsmI GAATGC 2 cut(s) 637, 823
BsnI GGCC 5 cut(s) 33, 95, 692, 913, 947
Bsp1286I GDGCHC 1 cut(s) 372
Bsp143I GATC 6 cut(s) 441, 489, 528, 619, 767, 925
Bsp19I CCATGG 1 cut(s) 78
BspANI GGCC 5 cut(s) 33, 95, 692, 913, 947
BspCNI CTCAG 3 cut(s) 615, 676, 702
BspFNI CGCG 2 cut(s) 413, 983
BspLI GGNNCC 5 cut(s) 34, 369, 463, 834, 912
BspPI GGATC 1 cut(s) 920
BspT107I GGYRCC 1 cut(s) 832
BsrDI GCAATG 2 cut(s) 555, 822
BsrFI RCCGGY 2 cut(s) 781, 947
BsrI ACTGG 2 cut(s) 437, 970
BssAI RCCGGY 2 cut(s) 781, 947
BssECI CCNNGG 5 cut(s) 28, 78, 90, 449, 903
BssHII GCGCGC 1 cut(s) 411
BssMI GATC 6 cut(s) 441, 489, 528, 619, 767, 925
BssNI GRCGYC 3 cut(s) 422, 510, 833
BssT1I CCWWGG 1 cut(s) 78
Bst2UI CCWGG 2 cut(s) 365, 694
Bst4CI ACNGT 1 cut(s) 904
BstACI GRCGYC 3 cut(s) 422, 510, 833
BstC8I GCNNGC 3 cut(s) 413, 690, 879
BstDEI CTNAG 3 cut(s) 623, 663, 710
BstDSI CCRYGG 2 cut(s) 78, 903
BstENI CCTNNNNNAGG 1 cut(s) 335
BstF5I GGATG 2 cut(s) 300, 874
BstFNI CGCG 2 cut(s) 413, 983
BstH2I RGCGCY 2 cut(s) 836, 884
BstHHI GCGC 5 cut(s) 413, 415, 835, 883, 985
BstKTI GATC 6 cut(s) 444, 492, 531, 622, 770, 928
BstMAI GTCTC 2 cut(s) 512, 595
BstMBI GATC 6 cut(s) 441, 489, 528, 619, 767, 925
BstMCI CGRYCG 5 cut(s) 96, 361, 770, 903, 948
BstMWI GCNNNNNNNGC 4 cut(s) 408, 673, 778, 799
BstNI CCWGG 2 cut(s) 365, 694
BstSCI CCNGG 5 cut(s) 90, 165, 189, 363, 692
BstUI CGCG 2 cut(s) 413, 983
BstV1I GCAGC 5 cut(s) 153, 535, 882, 997, 1036
BstZI CGGCCG 2 cut(s) 93, 945
Bsu36I CCTNAGG 1 cut(s) 710
BsuRI GGCC 5 cut(s) 33, 95, 692, 913, 947
BtgI CCRYGG 2 cut(s) 78, 903
BtgZI GCGATG 1 cut(s) 115
BtsCI GGATG 2 cut(s) 300, 874
BtsIMutI CAGTG 1 cut(s) 977
Cac8I GCNNGC 3 cut(s) 413, 690, 879
CaiI CAGNNNCTG 1 cut(s) 709
CfoI GCGC 5 cut(s) 413, 415, 835, 883, 985
Cfr10I RCCGGY 2 cut(s) 781, 947
Cfr13I GGNCC 3 cut(s) 32, 706, 911
CseI GACGC 2 cut(s) 499, 529
Csp6I GTAC 3 cut(s) 486, 715, 940
CviAII CATG 3 cut(s) 79, 259, 718
CviQI GTAC 3 cut(s) 486, 715, 940
DdeI CTNAG 3 cut(s) 623, 663, 710
DinI GGCGCC 1 cut(s) 834
DpnI GATC 6 cut(s) 443, 491, 530, 621, 769, 927
DpnII GATC 6 cut(s) 441, 489, 528, 619, 767, 925
DrdI GACNNNNNNGTC 1 cut(s) 353
DseDI GACNNNNNNGTC 1 cut(s) 353
EaeI YGGCCR 2 cut(s) 93, 945
EagI CGGCCG 2 cut(s) 93, 945
EciI GGCGGA 3 cut(s) 317, 583, 803
EclXI CGGCCG 2 cut(s) 93, 945
Eco130I CCWWGG 1 cut(s) 78
Eco24I GRGCYC 1 cut(s) 372
Eco47I GGWCC 1 cut(s) 706
Eco52I CGGCCG 2 cut(s) 93, 945
Eco81I CCTNAGG 1 cut(s) 710
EcoNI CCTNNNNNAGG 1 cut(s) 335
EcoO109I RGGNCCY 1 cut(s) 706
EcoRII CCWGG 2 cut(s) 363, 692
EcoT14I CCWWGG 1 cut(s) 78
EcoT38I GRGCYC 1 cut(s) 372
EgeI GGCGCC 1 cut(s) 834
EheI GGCGCC 1 cut(s) 834
ErhI CCWWGG 1 cut(s) 78
Esp3I CGTCTC 2 cut(s) 512, 595
FaeI CATG 3 cut(s) 82, 262, 721
FaiI YATR 6 cut(s) 80, 260, 650, 652, 719, 852
FaqI GGGAC 1 cut(s) 509
FatI CATG 3 cut(s) 78, 258, 717
FauI CCCGC 4 cut(s) 119, 134, 856, 901
FblI GTMKAC 2 cut(s) 171, 357
Fnu4HI GCNGC 9 cut(s) 96, 142, 416, 524, 836, 896, 981, 986, 1025
FokI GGATG 2 cut(s) 287, 881
FriOI GRGCYC 1 cut(s) 372
Fsp4HI GCNGC 9 cut(s) 96, 142, 416, 524, 836, 896, 981, 986, 1025
FspBI CTAG 2 cut(s) 573, 846
GlaI GCGC 5 cut(s) 412, 414, 834, 882, 984
GluI GCNGC 9 cut(s) 96, 142, 416, 524, 836, 896, 981, 986, 1025
GsuI CTGGAG 4 cut(s) 386, 451, 815, 953
HaeII RGCGCY 2 cut(s) 836, 884
HaeIII GGCC 5 cut(s) 33, 95, 692, 913, 947
HapII CCGG 9 cut(s) 92, 166, 191, 315, 668, 685, 782, 948, 989
HgaI GACGC 2 cut(s) 499, 529
HhaI GCGC 5 cut(s) 413, 415, 835, 883, 985
Hin1I GRCGYC 3 cut(s) 422, 510, 833
Hin1II CATG 3 cut(s) 82, 262, 721
Hin6I GCGC 5 cut(s) 411, 413, 833, 881, 983
HinP1I GCGC 5 cut(s) 411, 413, 833, 881, 983
HincII GTYRAC 2 cut(s) 172, 358
HindII GTYRAC 2 cut(s) 172, 358
HinfI GANTC 7 cut(s) 20, 56, 347, 456, 658, 727, 872
HpaII CCGG 9 cut(s) 92, 166, 191, 315, 668, 685, 782, 948, 989
HphI GGTGA 3 cut(s) 40, 619, 763
Hpy166II GTNNAC 3 cut(s) 172, 358, 550
Hpy188I TCNGA 3 cut(s) 461, 624, 732
Hpy188III TCNNGA 3 cut(s) 502, 517, 536
Hpy8I GTNNAC 3 cut(s) 172, 358, 550
Hpy99I CGWCG 7 cut(s) 140, 359, 515, 518, 602, 727, 778
HpyAV CCTTC 5 cut(s) 193, 228, 238, 499, 1027
HpyCH4III ACNGT 1 cut(s) 904
HpyCH4IV ACGT 1 cut(s) 422
HpyCH4V TGCA 1 cut(s) 1024
HpyF10VI GCNNNNNNNGC 4 cut(s) 408, 673, 778, 799
HpyF3I CTNAG 3 cut(s) 623, 663, 710
HpySE526I ACGT 1 cut(s) 422
Hsp92I GRCGYC 3 cut(s) 422, 510, 833
Hsp92II CATG 3 cut(s) 82, 262, 721
HspAI GCGC 5 cut(s) 411, 413, 833, 881, 983
KasI GGCGCC 1 cut(s) 832
Kzo9I GATC 6 cut(s) 441, 489, 528, 619, 767, 925
LmnI GCTCC 6 cut(s) 367, 634, 687, 790, 892, 972
Lsp1109I GCAGC 5 cut(s) 153, 535, 882, 997, 1036
LweI GCATC 4 cut(s) 138, 480, 768, 1011
MaeI CTAG 2 cut(s) 573, 846
MaeII ACGT 1 cut(s) 422
MaeIII GTNAC 2 cut(s) 46, 740
MalI GATC 6 cut(s) 443, 491, 530, 621, 769, 927
MboI GATC 6 cut(s) 441, 489, 528, 619, 767, 925
MhlI GDGCHC 1 cut(s) 372
Mly113I GGCGCC 1 cut(s) 833
MlyI GAGTC 4 cut(s) 50, 341, 667, 866
MmeI TCCRAC 2 cut(s) 186, 318
MseI TTAA 1 cut(s) 960
MspA1I CMGCKG 2 cut(s) 418, 526
MspI CCGG 9 cut(s) 92, 166, 191, 315, 668, 685, 782, 948, 989
MspR9I CCNGG 5 cut(s) 92, 167, 191, 365, 694
Mva1269I GAATGC 2 cut(s) 637, 823
MvaI CCWGG 2 cut(s) 365, 694
MvnI CGCG 2 cut(s) 413, 983
MwoI GCNNNNNNNGC 4 cut(s) 408, 673, 778, 799
NarI GGCGCC 1 cut(s) 833
NciI CCSGG 3 cut(s) 92, 167, 191
NcoI CCATGG 1 cut(s) 78
NdeII GATC 6 cut(s) 441, 489, 528, 619, 767, 925
NlaIII CATG 3 cut(s) 82, 262, 721
NlaIV GGNNCC 5 cut(s) 34, 369, 463, 834, 912
NmeAIII GCCGAG 3 cut(s) 9, 430, 923
NmuCI GTSAC 1 cut(s) 46
PauI GCGCGC 1 cut(s) 411
PcsI WCGNNNNNNNCGW 2 cut(s) 435, 507
PctI GAATGC 2 cut(s) 637, 823
PfeI GAWTC 3 cut(s) 20, 456, 727
PflMI CCANNNNNTGG 1 cut(s) 828
PkrI GCNGC 9 cut(s) 97, 143, 417, 525, 837, 897, 982, 987, 1026
Ple19I CGATCG 1 cut(s) 770
PleI GAGTC 4 cut(s) 50, 341, 666, 866
PluTI GGCGCC 1 cut(s) 836
PpsI GAGTC 4 cut(s) 50, 341, 666, 866
PpuMI RGGWCCY 1 cut(s) 706
PshBI ATTAAT 1 cut(s) 960
Psp5II RGGWCCY 1 cut(s) 706
Psp6I CCWGG 2 cut(s) 363, 692
PspGI CCWGG 2 cut(s) 363, 692
PspN4I GGNNCC 5 cut(s) 34, 369, 463, 834, 912
PspPI GGNCC 3 cut(s) 32, 706, 911
PspPPI RGGWCCY 1 cut(s) 706
PstNI CAGNNNCTG 1 cut(s) 709
PteI GCGCGC 1 cut(s) 411
PvuI CGATCG 1 cut(s) 770
PvuII CAGCTG 1 cut(s) 526
RsaI GTAC 3 cut(s) 487, 716, 941
RsaNI GTAC 3 cut(s) 486, 715, 940
SalI GTCGAC 2 cut(s) 170, 356
SaqAI TTAA 1 cut(s) 960
SatI GCNGC 9 cut(s) 96, 142, 416, 524, 836, 896, 981, 986, 1025
Sau3AI GATC 6 cut(s) 441, 489, 528, 619, 767, 925
Sau96I GGNCC 3 cut(s) 32, 706, 911
SchI GAGTC 4 cut(s) 50, 341, 667, 866
ScrFI CCNGG 5 cut(s) 92, 167, 191, 365, 694
SduI GDGCHC 1 cut(s) 372
SfaNI GCATC 4 cut(s) 138, 480, 768, 1011
SfoI GGCGCC 1 cut(s) 834
SinI GGWCC 1 cut(s) 706
SspDI GGCGCC 1 cut(s) 832
SspMI CTAG 2 cut(s) 573, 846
StyD4I CCNGG 5 cut(s) 90, 165, 189, 363, 692
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 904
TaiI ACGT 1 cut(s) 425
TatI WGTACW 1 cut(s) 939
TauI GCSGC 4 cut(s) 98, 418, 838, 983
TfiI GAWTC 3 cut(s) 20, 456, 727
Tru1I TTAA 1 cut(s) 960
Tru9I TTAA 1 cut(s) 960
TscAI CASTG 1 cut(s) 977
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 5 cut(s) 141, 523, 895, 985, 1024
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 1 cut(s) 136
TspGWI ACGGA 4 cut(s) 34, 343, 367, 577
TspRI CASTG 1 cut(s) 977
Van91I CCANNNNNTGG 1 cut(s) 828
VpaK11BI GGWCC 1 cut(s) 706
VspI ATTAAT 1 cut(s) 960
XagI CCTNNNNNAGG 1 cut(s) 335
XmiI GTMKAC 2 cut(s) 171, 357
XspI CTAG 2 cut(s) 573, 846
ZraI GACGTC 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.