Rh1AG462700

Protein TRANSPARENT TESTA GLABRA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0006319
Physical Location & Seq
Reverse (-)
67889065 .. 67891644
2580 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG462700.1

Sequence Viewer

Length: 1041 bp
ATGGAGAACTCGACCCAAGAATCCCACCTCGGCCCGGAAAACTCCGTCACCTACGACTCCCCCTACCCGCTCTACGCCATGGCGTTTTCCCCGGCCGCCCCGACCCGAACCCGCCACAACCACCCCCGCATCGCCGTCGGCAGCTTCATCGAGGAGTTCTCCAACCGGGTCGACATTCTCTCGTTCGACCCGGACACCCTAACCCTAAAGCCCAACCCTTCCCTCTCCTTCGACCACCCCTACCCTCCCACCAAGCTCATGTTCCACCCAAACCCTAACTCCCTCCACAAGTCCAACGACATCCTCGCCTCCTCCGGCGACTACCTCCGCCTCTGGGAGGTCAAAGACTCCTCCGTCGACCGCCTCGAGCCCATCTCCGTCCTCAACAACTCCAAGACCAGCGAGTTCTGCGCGCCGCTGACGTCATTCGACTGGAACGAGATCGAGCCCCGGCGCATCGGAACCTCCAGCATCGACACCACCTGTACGATTTGGGACATCGAGAAGGGCGTCGTCGAGACGCAGCTGATCGCTCACGATAAGGAGGTTTACGACATTGCGTGGGGCGAAGCTAGGGTTTTCGCCTCCGTCTCCGCCGACGGGTCGGTCAGGATTTTTGATCTGAGGGACAAGGAGCATTCCACGATTATATACGAGAGTCCTCAGCCGGACACGCCGCTGCTCCGGCTGGCCTGGAACAAGCAGGACCTGAGGTACATGGCGACGATTCTGATGGATAGTAACAAGGTGGTGATATTGGACATTCGATCGCCGACGATGCCGGTGGCGGAGCTGGAGAGGCACAGGGGGAGTGTGAATGCCATTGCGTGGGCGCCGCAGAGTGCTAGGCATATTTGTTCGGCGGGGGATGACTCGCAGGCGCTTATTTGGGAGCTGCCGACGGTGGCGGGGCCGAATGGGATTGATCCAATGTCAATGTACTCGGCCGGCGCGGAGATTAATCAGCTCCAGTGGTCTGCCGCGCAGCCGGATTGGATTGCCATTGCGTTTAGTAATAAGATGCAGCTGTTGAAGGTTTGA

Protein Analysis

346

Amino Acids

38.74

Weight (kDa)

5.0

Isoelectric Point (pI)

54.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EIPR1 PF23609 34 - 297 1.8e-07 EIPR1 beta-propeller
Beta-prop_TEP1_2nd PF25047 94 - 212 4.9e-06 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 96 - 329 2.8e-19 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 136 - 339 1.8e-10 WDHD1 first WD40 domain
WD40_Prp19 PF24814 141 - 217 7.4e-08 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 142 - 301 1.3e-16 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 151 - 214 1.4e-06 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 151 - 211 5.6e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 152 - 213 3.6e-09 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 152 - 220 7.9e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 164 - 301 5.9e-09 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 353, 605
AatII GACGTC 1 cut(s) 425
AccB1I GGYRCC 1 cut(s) 832
AccB7I CCANNNNNTGG 1 cut(s) 828
AccBSI CCGCTC 1 cut(s) 70
AccI GTMKAC 2 cut(s) 171, 357
AccII CGCG 3 cut(s) 413, 953, 983
AclWI GGATC 1 cut(s) 920
AcoI YGGCCR 2 cut(s) 93, 945
AcyI GRCGYC 3 cut(s) 422, 510, 833
AfaI GTAC 3 cut(s) 487, 716, 941
AfiI CCNNNNNNNGG 5 cut(s) 34, 334, 337, 600, 828
AgsI TTSAA 1 cut(s) 1033
AjnI CCWGG 1 cut(s) 692
AjuI GAANNNNNNNTTGG 2 cut(s) 245, 277
AluBI AGCT 8 cut(s) 144, 256, 526, 572, 793, 895, 967, 1027
AluI AGCT 8 cut(s) 144, 256, 526, 572, 793, 895, 967, 1027
Alw26I GTCTC 2 cut(s) 512, 595
AlwI GGATC 1 cut(s) 920
AlwNI CAGNNNCTG 1 cut(s) 709
Ama87I CYCGRG 1 cut(s) 365
AoxI GGCC 5 cut(s) 31, 93, 690, 911, 945
ApeKI GCWGC 6 cut(s) 141, 523, 679, 895, 985, 1024
AseI ATTAAT 1 cut(s) 960
AspLEI GCGC 7 cut(s) 413, 415, 456, 835, 883, 953, 985
AspS9I GGNCC 3 cut(s) 32, 706, 911
AsuC2I CCSGG 5 cut(s) 35, 92, 167, 191, 451
AsuHPI GGTGA 2 cut(s) 40, 763
AvaI CYCGRG 1 cut(s) 365
AvaII GGWCC 1 cut(s) 706
AxyI CCTNAGG 1 cut(s) 710
BanI GGYRCC 1 cut(s) 832
BanII GRGCYC 2 cut(s) 372, 450
BbvCI CCTCAGC 1 cut(s) 663
BbvI GCAGC 6 cut(s) 153, 535, 666, 882, 997, 1036
BccI CCATC 2 cut(s) 380, 727
BceAI ACGGC 1 cut(s) 119
BcgI CGANNNNNNTGC 4 cut(s) 118, 130, 152, 164
BciT130I CCWGG 1 cut(s) 694
BcnI CCSGG 5 cut(s) 35, 92, 167, 191, 451
BcoDI GTCTC 2 cut(s) 512, 595
BfaI CTAG 2 cut(s) 573, 846
BfoI RGCGCY 2 cut(s) 836, 884
Bme1390I CCNGG 6 cut(s) 35, 92, 167, 191, 451, 694
Bme18I GGWCC 1 cut(s) 706
BmeT110I CYCGRG 1 cut(s) 365
BmgT120I GGNCC 3 cut(s) 32, 706, 911
BmiI GGNNCC 3 cut(s) 463, 834, 912
BmrFI CCNGG 6 cut(s) 35, 92, 167, 191, 451, 694
BmsI GCATC 5 cut(s) 138, 465, 480, 768, 1011
BplI GAGNNNNNCTC 4 cut(s) 143, 175, 359, 391
BpmI CTGGAG 3 cut(s) 451, 815, 953
Bpu10I CCTNAGC 1 cut(s) 663
BpuMI CCSGG 5 cut(s) 35, 92, 167, 191, 451
BsaHI GRCGYC 3 cut(s) 422, 510, 833
BsaJI CCNNGG 4 cut(s) 28, 78, 90, 449
BsaXI ACNNNNNCTCC 2 cut(s) 263, 293
Bsc4I CCNNNNNNNGG 5 cut(s) 34, 334, 337, 600, 828
Bse118I RCCGGY 2 cut(s) 781, 947
Bse1I ACTGG 2 cut(s) 437, 970
Bse21I CCTNAGG 1 cut(s) 710
Bse3DI GCAATG 3 cut(s) 555, 822, 1002
BseBI CCWGG 1 cut(s) 694
BseDI CCNNGG 4 cut(s) 28, 78, 90, 449
BseGI GGATG 2 cut(s) 300, 874
BseLI CCNNNNNNNGG 5 cut(s) 34, 334, 337, 600, 828
BseMI GCAATG 3 cut(s) 555, 822, 1002
BseMII CTCAG 3 cut(s) 614, 677, 701
BseNI ACTGG 2 cut(s) 437, 970
BsePI GCGCGC 1 cut(s) 411
BseRI GAGGAG 3 cut(s) 167, 301, 340
BseX3I CGGCCG 2 cut(s) 93, 945
BseXI GCAGC 6 cut(s) 153, 535, 666, 882, 997, 1036
Bsh1236I CGCG 3 cut(s) 413, 953, 983
Bsh1285I CGRYCG 4 cut(s) 96, 361, 770, 948
BshFI GGCC 5 cut(s) 33, 95, 692, 913, 947
BshNI GGYRCC 1 cut(s) 832
BsiEI CGRYCG 4 cut(s) 96, 361, 770, 948
BsiHKCI CYCGRG 1 cut(s) 365
BslFI GGGAC 2 cut(s) 509, 641
BslI CCNNNNNNNGG 5 cut(s) 34, 334, 337, 600, 828
BsmAI GTCTC 2 cut(s) 512, 595
BsmBI CGTCTC 2 cut(s) 512, 595
BsmFI GGGAC 2 cut(s) 509, 641
BsmI GAATGC 2 cut(s) 637, 823
BsnI GGCC 5 cut(s) 33, 95, 692, 913, 947
BsoBI CYCGRG 1 cut(s) 365
Bsp1286I GDGCHC 2 cut(s) 372, 450
Bsp143I GATC 5 cut(s) 441, 528, 619, 767, 925
Bsp19I CCATGG 1 cut(s) 78
BspANI GGCC 5 cut(s) 33, 95, 692, 913, 947
BspCNI CTCAG 3 cut(s) 615, 676, 702
BspFNI CGCG 3 cut(s) 413, 953, 983
BspLI GGNNCC 3 cut(s) 463, 834, 912
BspPI GGATC 1 cut(s) 920
BspT107I GGYRCC 1 cut(s) 832
BsrBI CCGCTC 1 cut(s) 70
BsrDI GCAATG 3 cut(s) 555, 822, 1002
BsrFI RCCGGY 2 cut(s) 781, 947
BsrI ACTGG 2 cut(s) 437, 970
BssAI RCCGGY 2 cut(s) 781, 947
BssECI CCNNGG 4 cut(s) 28, 78, 90, 449
BssHII GCGCGC 1 cut(s) 411
BssMI GATC 5 cut(s) 441, 528, 619, 767, 925
BssNI GRCGYC 3 cut(s) 422, 510, 833
BssT1I CCWWGG 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 694
Bst4CI ACNGT 1 cut(s) 904
BstACI GRCGYC 3 cut(s) 422, 510, 833
BstC8I GCNNGC 4 cut(s) 413, 690, 879, 949
BstDEI CTNAG 3 cut(s) 623, 663, 710
BstDSI CCRYGG 1 cut(s) 78
BstENI CCTNNNNNAGG 1 cut(s) 335
BstF5I GGATG 2 cut(s) 300, 874
BstFNI CGCG 3 cut(s) 413, 953, 983
BstH2I RGCGCY 2 cut(s) 836, 884
BstHHI GCGC 7 cut(s) 413, 415, 456, 835, 883, 953, 985
BstKTI GATC 5 cut(s) 444, 531, 622, 770, 928
BstMAI GTCTC 2 cut(s) 512, 595
BstMBI GATC 5 cut(s) 441, 528, 619, 767, 925
BstMCI CGRYCG 4 cut(s) 96, 361, 770, 948
BstMWI GCNNNNNNNGC 5 cut(s) 408, 673, 685, 778, 799
BstNI CCWGG 1 cut(s) 694
BstSCI CCNGG 6 cut(s) 33, 90, 165, 189, 449, 692
BstUI CGCG 3 cut(s) 413, 953, 983
BstV1I GCAGC 6 cut(s) 153, 535, 666, 882, 997, 1036
BstZI CGGCCG 2 cut(s) 93, 945
Bsu36I CCTNAGG 1 cut(s) 710
BsuRI GGCC 5 cut(s) 33, 95, 692, 913, 947
BtgI CCRYGG 1 cut(s) 78
BtgZI GCGATG 1 cut(s) 115
BtsCI GGATG 2 cut(s) 300, 874
BtsIMutI CAGTG 1 cut(s) 977
Cac8I GCNNGC 4 cut(s) 413, 690, 879, 949
CaiI CAGNNNCTG 1 cut(s) 709
CfoI GCGC 7 cut(s) 413, 415, 456, 835, 883, 953, 985
Cfr10I RCCGGY 2 cut(s) 781, 947
Cfr13I GGNCC 3 cut(s) 32, 706, 911
CseI GACGC 2 cut(s) 499, 529
Csp6I GTAC 3 cut(s) 486, 715, 940
CviAII CATG 3 cut(s) 79, 259, 718
CviQI GTAC 3 cut(s) 486, 715, 940
DdeI CTNAG 3 cut(s) 623, 663, 710
DinI GGCGCC 1 cut(s) 834
DpnI GATC 5 cut(s) 443, 530, 621, 769, 927
DpnII GATC 5 cut(s) 441, 528, 619, 767, 925
DrdI GACNNNNNNGTC 2 cut(s) 353, 605
DseDI GACNNNNNNGTC 2 cut(s) 353, 605
EaeI YGGCCR 2 cut(s) 93, 945
EagI CGGCCG 2 cut(s) 93, 945
EciI GGCGGA 3 cut(s) 317, 583, 803
EclXI CGGCCG 2 cut(s) 93, 945
Eco130I CCWWGG 1 cut(s) 78
Eco24I GRGCYC 2 cut(s) 372, 450
Eco47I GGWCC 1 cut(s) 706
Eco52I CGGCCG 2 cut(s) 93, 945
Eco81I CCTNAGG 1 cut(s) 710
Eco88I CYCGRG 1 cut(s) 365
EcoNI CCTNNNNNAGG 1 cut(s) 335
EcoO109I RGGNCCY 1 cut(s) 706
EcoRII CCWGG 1 cut(s) 692
EcoT14I CCWWGG 1 cut(s) 78
EcoT38I GRGCYC 2 cut(s) 372, 450
EgeI GGCGCC 1 cut(s) 834
EheI GGCGCC 1 cut(s) 834
ErhI CCWWGG 1 cut(s) 78
Esp3I CGTCTC 2 cut(s) 512, 595
FaeI CATG 3 cut(s) 82, 262, 721
FaiI YATR 6 cut(s) 80, 260, 650, 652, 719, 852
FaqI GGGAC 2 cut(s) 509, 641
FatI CATG 3 cut(s) 78, 258, 717
FauI CCCGC 5 cut(s) 75, 119, 134, 856, 901
FblI GTMKAC 2 cut(s) 171, 357
FokI GGATG 2 cut(s) 287, 881
FriOI GRGCYC 2 cut(s) 372, 450
FspBI CTAG 2 cut(s) 573, 846
GlaI GCGC 7 cut(s) 412, 414, 455, 834, 882, 952, 984
GsuI CTGGAG 3 cut(s) 451, 815, 953
HaeII RGCGCY 2 cut(s) 836, 884
HaeIII GGCC 5 cut(s) 33, 95, 692, 913, 947
HgaI GACGC 2 cut(s) 499, 529
HhaI GCGC 7 cut(s) 413, 415, 456, 835, 883, 953, 985
Hin1I GRCGYC 3 cut(s) 422, 510, 833
Hin1II CATG 3 cut(s) 82, 262, 721
Hin6I GCGC 7 cut(s) 411, 413, 454, 833, 881, 951, 983
HinP1I GCGC 7 cut(s) 411, 413, 454, 833, 881, 951, 983
HincII GTYRAC 2 cut(s) 172, 358
HindII GTYRAC 2 cut(s) 172, 358
HinfI GANTC 6 cut(s) 20, 56, 347, 658, 727, 872
HphI GGTGA 2 cut(s) 40, 763
Hpy166II GTNNAC 3 cut(s) 172, 358, 550
Hpy188I TCNGA 3 cut(s) 461, 624, 732
Hpy188III TCNNGA 4 cut(s) 502, 517, 536, 610
Hpy8I GTNNAC 3 cut(s) 172, 358, 550
Hpy99I CGWCG 8 cut(s) 140, 359, 515, 518, 602, 727, 778, 904
HpyAV CCTTC 4 cut(s) 228, 238, 499, 1027
HpyCH4III ACNGT 1 cut(s) 904
HpyCH4IV ACGT 1 cut(s) 422
HpyCH4V TGCA 1 cut(s) 1024
HpyF10VI GCNNNNNNNGC 5 cut(s) 408, 673, 685, 778, 799
HpyF3I CTNAG 3 cut(s) 623, 663, 710
HpySE526I ACGT 1 cut(s) 422
Hsp92I GRCGYC 3 cut(s) 422, 510, 833
Hsp92II CATG 3 cut(s) 82, 262, 721
HspAI GCGC 7 cut(s) 411, 413, 454, 833, 881, 951, 983
KasI GGCGCC 1 cut(s) 832
KroI GCCGGC 1 cut(s) 947
KroNI GCCGGC 1 cut(s) 949
Kzo9I GATC 5 cut(s) 441, 528, 619, 767, 925
LmnI GCTCC 5 cut(s) 634, 687, 790, 892, 972
Lsp1109I GCAGC 6 cut(s) 153, 535, 666, 882, 997, 1036
LweI GCATC 5 cut(s) 138, 465, 480, 768, 1011
MaeI CTAG 2 cut(s) 573, 846
MaeII ACGT 1 cut(s) 422
MaeIII GTNAC 2 cut(s) 46, 740
MalI GATC 5 cut(s) 443, 530, 621, 769, 927
MbiI CCGCTC 1 cut(s) 70
MboI GATC 5 cut(s) 441, 528, 619, 767, 925
MhlI GDGCHC 2 cut(s) 372, 450
Mly113I GGCGCC 1 cut(s) 833
MlyI GAGTC 4 cut(s) 50, 341, 667, 866
MmeI TCCRAC 2 cut(s) 186, 318
MroNI GCCGGC 1 cut(s) 947
MseI TTAA 1 cut(s) 960
MspA1I CMGCKG 4 cut(s) 418, 526, 679, 1027
MspR9I CCNGG 6 cut(s) 35, 92, 167, 191, 451, 694
Mva1269I GAATGC 2 cut(s) 637, 823
MvaI CCWGG 1 cut(s) 694
MvnI CGCG 3 cut(s) 413, 953, 983
MwoI GCNNNNNNNGC 5 cut(s) 408, 673, 685, 778, 799
NaeI GCCGGC 1 cut(s) 949
NarI GGCGCC 1 cut(s) 833
NciI CCSGG 5 cut(s) 35, 92, 167, 191, 451
NcoI CCATGG 1 cut(s) 78
NdeII GATC 5 cut(s) 441, 528, 619, 767, 925
NgoMIV GCCGGC 1 cut(s) 947
NlaIII CATG 3 cut(s) 82, 262, 721
NlaIV GGNNCC 3 cut(s) 463, 834, 912
NmeAIII GCCGAG 2 cut(s) 9, 923
NmuCI GTSAC 1 cut(s) 46
PaeR7I CTCGAG 1 cut(s) 365
PauI GCGCGC 1 cut(s) 411
PcsI WCGNNNNNNNCGW 3 cut(s) 363, 435, 507
PctI GAATGC 2 cut(s) 637, 823
PdiI GCCGGC 1 cut(s) 949
PfeI GAWTC 2 cut(s) 20, 727
PflMI CCANNNNNTGG 1 cut(s) 828
Ple19I CGATCG 1 cut(s) 770
PleI GAGTC 4 cut(s) 50, 341, 666, 866
PluTI GGCGCC 1 cut(s) 836
PpsI GAGTC 4 cut(s) 50, 341, 666, 866
PpuMI RGGWCCY 1 cut(s) 706
PshBI ATTAAT 1 cut(s) 960
Psp5II RGGWCCY 1 cut(s) 706
Psp6I CCWGG 1 cut(s) 692
PspGI CCWGG 1 cut(s) 692
PspN4I GGNNCC 3 cut(s) 463, 834, 912
PspPI GGNCC 3 cut(s) 32, 706, 911
PspPPI RGGWCCY 1 cut(s) 706
PspXI VCTCGAGB 1 cut(s) 365
PstNI CAGNNNCTG 1 cut(s) 709
PteI GCGCGC 1 cut(s) 411
PvuI CGATCG 1 cut(s) 770
PvuII CAGCTG 2 cut(s) 526, 1027
RsaI GTAC 3 cut(s) 487, 716, 941
RsaNI GTAC 3 cut(s) 486, 715, 940
SalI GTCGAC 2 cut(s) 170, 356
SaqAI TTAA 1 cut(s) 960
Sau3AI GATC 5 cut(s) 441, 528, 619, 767, 925
Sau96I GGNCC 3 cut(s) 32, 706, 911
SchI GAGTC 4 cut(s) 50, 341, 667, 866
ScrFI CCNGG 6 cut(s) 35, 92, 167, 191, 451, 694
SduI GDGCHC 2 cut(s) 372, 450
SfaNI GCATC 5 cut(s) 138, 465, 480, 768, 1011
SfoI GGCGCC 1 cut(s) 834
Sfr274I CTCGAG 1 cut(s) 365
SinI GGWCC 1 cut(s) 706
SlaI CTCGAG 1 cut(s) 365
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
SspDI GGCGCC 1 cut(s) 832
SspMI CTAG 2 cut(s) 573, 846
StyD4I CCNGG 6 cut(s) 33, 90, 165, 189, 449, 692
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 904
TaiI ACGT 1 cut(s) 425
TaqII GACCGA 1 cut(s) 595
TatI WGTACW 1 cut(s) 939
TauI GCSGC 5 cut(s) 98, 418, 679, 838, 983
TfiI GAWTC 2 cut(s) 20, 727
Tru1I TTAA 1 cut(s) 960
Tru9I TTAA 1 cut(s) 960
TscAI CASTG 1 cut(s) 977
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 6 cut(s) 141, 523, 679, 895, 985, 1024
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 1 cut(s) 136
TspGWI ACGGA 4 cut(s) 34, 343, 367, 577
TspRI CASTG 1 cut(s) 977
Van91I CCANNNNNTGG 1 cut(s) 828
VpaK11BI GGWCC 1 cut(s) 706
VspI ATTAAT 1 cut(s) 960
XagI CCTNNNNNAGG 1 cut(s) 335
XhoI CTCGAG 1 cut(s) 365
XmiI GTMKAC 2 cut(s) 171, 357
XspI CTAG 2 cut(s) 573, 846
ZraI GACGTC 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.