pycom01g23670

Protein TRANSPARENT TESTA GLABRA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0006319
Physical Location & Seq
Reverse (-)
20927381 .. 20928409
1029 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g23670.1

Sequence Viewer

Length: 1029 bp
ATGGAGAACTCTACGCAAGAATCGCATCTCAAGGCGGAGAACTCGGTGACGTACGAGTCGCCTTACCCGCTGTACGCCTTGGCGTTTGCCTCGCCCCAAACCCGAACCCGCCACCAGCACCACCGAATCGCCGTCGGCAGCTTTATCGAGGAGTACTCGAACCGGGTGGACATCCTTTCCTTCGACCCGGATACCCTTTCAATAAAGCCGAACCCGACCCTCTCCTTCGACCACCCTTATCCGCCCACCAAGCTCATGTTCCACCCGAACCCCAACGCCCTCCACAAAACCAACGACGTCTTAGCTTCCTCTGGCGACTACCTCCGCCTTTGGGAGGTCGGTGACTCCACCGTCGAGCCTATCCAGGTGTTAAATAACAGCAAGACCAGCGAGTTTTGCGCTCCGCTGACGTCATTCGATTGGAACGACATTGAGCCCCGGCGAATTGGGACTTCCAGCATCGACACCACCTGCACAATTTGGGACATCGAAAAGGGGGTCGTCGAGACGCAGCTGATTGCTCACGATAAAGAGGTGTACGACATCGCGTGGGGGGAAGCTAGGGTTTTTGCTTCGGTTTCGGCTGATGGGTCGGTGAGAATTTTCGATTTGAGGGACAAGGAGCACTCCACTATCATCTATGAGAGTCCTCAGCCAGATACCCCTTTGCTTCGATTGGCTTGGAACAAGCAGGATTTGAGGTACATGGCCACAATTTTGATGGACAGCAATAAAGTTGTGATCTTGGACATCCGATCGCCGACGATGCCAGTGGCGGAGCTGGAGAGGCACAGAGGTAGTGTGAATGCTATTGCTTGGGCCCCCCAGAGTTGTAGGCACATTTGCTCTGCTGGGGATGACACTCAGGCGCTTATTTGGGACCTGCCCACGGTCGCTGGGCCGAATGGAATCGACCCCATGTCGATGTACTCCGCAGGTGCGGAGATTAATCAGCTGCAGTGGTCTGCTGCACAGCCTGATTGGATTTCCATTGCATTTTCTAACAAGATGCAGCTTTTGAAGGTTTGA

Protein Analysis

343

Amino Acids

38.43

Weight (kDa)

5.05

Isoelectric Point (pI)

48.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 96 - 329 4e-19 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 134 - 336 2e-10 WDHD1 first WD40 domain
WD40_Prp19 PF24814 137 - 213 8.3e-08 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 138 - 298 7.4e-17 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 146 - 211 8e-07 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 147 - 207 5.3e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 148 - 209 3.2e-09 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 149 - 216 7.8e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 174 - 298 4.6e-06 WDR36/Utp21 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 479, 926
AasI GACNNNNNNGTC 2 cut(s) 55, 350
AatII GACGTC 2 cut(s) 300, 413
Acc36I ACCTGC 3 cut(s) 479, 891, 926
AccII CGCG 1 cut(s) 548
AciI CCGC 9 cut(s) 35, 68, 109, 242, 325, 404, 776, 933, 941
AcoI YGGCCR 1 cut(s) 708
AcsI RAATTY 1 cut(s) 600
AcyI GRCGYC 2 cut(s) 297, 410
AfaI GTAC 6 cut(s) 53, 74, 155, 539, 704, 929
AfiI CCNNNNNNNGG 3 cut(s) 331, 334, 889
AgsI TTSAA 2 cut(s) 201, 1021
AhdI GACNNNNNGTC 1 cut(s) 919
AjnI CCWGG 1 cut(s) 363
AjuI GAANNNNNNNTTGG 2 cut(s) 242, 274
AluBI AGCT 8 cut(s) 141, 253, 305, 514, 560, 781, 955, 1015
AluI AGCT 8 cut(s) 141, 253, 305, 514, 560, 781, 955, 1015
Alw21I GWGCWC 1 cut(s) 627
Alw26I GTCTC 1 cut(s) 500
AoxI GGCC 3 cut(s) 708, 819, 899
ApaI GGGCCC 1 cut(s) 823
ApeKI GCWGC 5 cut(s) 138, 511, 955, 968, 1012
ApoI RAATTY 1 cut(s) 600
ArsI GACNNNNNNTTYG 2 cut(s) 483, 515
AseI ATTAAT 1 cut(s) 948
AspLEI GCGC 2 cut(s) 401, 871
AspS9I GGNCC 4 cut(s) 819, 820, 880, 899
AsuC2I CCSGG 3 cut(s) 164, 188, 439
AsuHPI GGTGA 3 cut(s) 58, 353, 607
AvaII GGWCC 1 cut(s) 880
BaeGI GKGCMC 1 cut(s) 823
BalI TGGCCA 1 cut(s) 710
BanII GRGCYC 2 cut(s) 438, 823
Bbv12I GWGCWC 1 cut(s) 627
BbvCI CCTCAGC 1 cut(s) 651
BbvI GCAGC 5 cut(s) 150, 523, 942, 955, 1024
BccI CCATC 2 cut(s) 581, 715
BceAI ACGGC 1 cut(s) 116
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 1 cut(s) 365
BciVI GTATCC 1 cut(s) 184
BcnI CCSGG 3 cut(s) 164, 188, 439
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 1 cut(s) 561
BfmI CTRYAG 1 cut(s) 956
BfoI RGCGCY 1 cut(s) 872
BfuAI ACCTGC 3 cut(s) 479, 891, 926
BfuI GTATCC 1 cut(s) 184
BisI GCNGC 5 cut(s) 139, 512, 956, 969, 1013
BlsI GCNGC 5 cut(s) 140, 513, 957, 970, 1014
BmcAI AGTACT 1 cut(s) 155
Bme1390I CCNGG 4 cut(s) 164, 188, 365, 439
Bme18I GGWCC 1 cut(s) 880
BmeRI GACNNNNNGTC 1 cut(s) 919
BmgT120I GGNCC 4 cut(s) 819, 820, 880, 899
BmiI GGNNCC 3 cut(s) 821, 822, 881
BmrFI CCNGG 4 cut(s) 164, 188, 365, 439
BmsI GCATC 4 cut(s) 34, 468, 756, 999
BplI GAGNNNNNCTC 2 cut(s) 140, 172
BpmI CTGGAG 1 cut(s) 803
Bpu10I CCTNAGC 1 cut(s) 651
BpuEI CTTGAG 1 cut(s) 14
BpuMI CCSGG 3 cut(s) 164, 188, 439
BsaHI GRCGYC 2 cut(s) 297, 410
BsaJI CCNNGG 3 cut(s) 78, 437, 888
Bsc4I CCNNNNNNNGG 3 cut(s) 331, 334, 889
Bse1I ACTGG 1 cut(s) 770
Bse3DI GCAATG 1 cut(s) 990
BseBI CCWGG 1 cut(s) 365
BseDI CCNNGG 3 cut(s) 78, 437, 888
BseGI GGATG 3 cut(s) 171, 750, 862
BseLI CCNNNNNNNGG 3 cut(s) 331, 334, 889
BseMI GCAATG 1 cut(s) 990
BseMII CTCAG 2 cut(s) 665, 878
BseNI ACTGG 1 cut(s) 770
BseRI GAGGAG 1 cut(s) 164
BseSI GKGCMC 1 cut(s) 823
BseXI GCAGC 5 cut(s) 150, 523, 942, 955, 1024
BseYI CCCAGC 2 cut(s) 851, 896
BsgI GTGCAG 2 cut(s) 457, 954
Bsh1236I CGCG 1 cut(s) 548
Bsh1285I CGRYCG 2 cut(s) 758, 894
BshFI GGCC 3 cut(s) 710, 821, 901
BsiEI CGRYCG 2 cut(s) 758, 894
BsiHKAI GWGCWC 1 cut(s) 627
BsiSI CCGG 3 cut(s) 163, 188, 439
BsiWI CGTACG 1 cut(s) 51
BslFI GGGAC 4 cut(s) 463, 497, 629, 893
BslI CCNNNNNNNGG 3 cut(s) 331, 334, 889
BsmAI GTCTC 1 cut(s) 500
BsmBI CGTCTC 1 cut(s) 500
BsmFI GGGAC 4 cut(s) 463, 497, 629, 893
BsmI GAATGC 1 cut(s) 811
BsnI GGCC 3 cut(s) 710, 821, 901
Bsp120I GGGCCC 1 cut(s) 819
Bsp1286I GDGCHC 3 cut(s) 438, 627, 823
Bsp143I GATC 2 cut(s) 741, 755
BspACI CCGC 9 cut(s) 35, 68, 109, 242, 325, 404, 776, 933, 941
BspANI GGCC 3 cut(s) 710, 821, 901
BspCNI CTCAG 2 cut(s) 664, 877
BspFNI CGCG 1 cut(s) 548
BspLI GGNNCC 3 cut(s) 821, 822, 881
BspMAI CTGCAG 1 cut(s) 960
BspMI ACCTGC 3 cut(s) 479, 891, 926
BsrDI GCAATG 1 cut(s) 990
BsrI ACTGG 1 cut(s) 770
BssECI CCNNGG 3 cut(s) 78, 437, 888
BssMI GATC 2 cut(s) 741, 755
BssNI GRCGYC 2 cut(s) 297, 410
BssT1I CCWWGG 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 365
Bst4CI ACNGT 2 cut(s) 352, 892
BstACI GRCGYC 2 cut(s) 297, 410
BstDEI CTNAG 3 cut(s) 301, 651, 864
BstDSI CCRYGG 1 cut(s) 888
BstENI CCTNNNNNAGG 1 cut(s) 332
BstF5I GGATG 3 cut(s) 171, 750, 862
BstFNI CGCG 1 cut(s) 548
BstH2I RGCGCY 1 cut(s) 872
BstHHI GCGC 2 cut(s) 401, 871
BstKTI GATC 2 cut(s) 744, 758
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 2 cut(s) 741, 755
BstMCI CGRYCG 2 cut(s) 758, 894
BstMWI GCNNNNNNNGC 7 cut(s) 22, 67, 250, 387, 396, 766, 787
BstNI CCWGG 1 cut(s) 365
BstSCI CCNGG 4 cut(s) 162, 186, 363, 437
BstSFI CTRYAG 1 cut(s) 956
BstSLI GKGCMC 1 cut(s) 823
BstUI CGCG 1 cut(s) 548
BstV1I GCAGC 5 cut(s) 150, 523, 942, 955, 1024
BsuI GTATCC 1 cut(s) 184
BsuRI GGCC 3 cut(s) 710, 821, 901
BtgI CCRYGG 1 cut(s) 888
BtgZI GCGATG 1 cut(s) 529
BtsCI GGATG 3 cut(s) 171, 750, 862
BtsI GCAGTG 1 cut(s) 965
BtsIMutI CAGTG 2 cut(s) 777, 965
BveI ACCTGC 3 cut(s) 479, 891, 926
CfoI GCGC 2 cut(s) 401, 871
Cfr13I GGNCC 4 cut(s) 819, 820, 880, 899
CseI GACGC 1 cut(s) 517
Csp6I GTAC 6 cut(s) 52, 73, 154, 538, 703, 928
CviAII CATG 3 cut(s) 256, 706, 919
CviQI GTAC 6 cut(s) 52, 73, 154, 538, 703, 928
DdeI CTNAG 3 cut(s) 301, 651, 864
DpnI GATC 2 cut(s) 743, 757
DpnII GATC 2 cut(s) 741, 755
DrdI GACNNNNNNGTC 2 cut(s) 55, 350
DriI GACNNNNNGTC 1 cut(s) 919
DseDI GACNNNNNNGTC 2 cut(s) 55, 350
EaeI YGGCCR 1 cut(s) 708
Eam1105I GACNNNNNGTC 1 cut(s) 919
EciI GGCGGA 4 cut(s) 50, 231, 314, 791
Eco130I CCWWGG 1 cut(s) 78
Eco24I GRGCYC 2 cut(s) 438, 823
Eco47I GGWCC 1 cut(s) 880
EcoNI CCTNNNNNAGG 1 cut(s) 332
EcoO109I RGGNCCY 2 cut(s) 820, 880
EcoRII CCWGG 1 cut(s) 363
EcoT14I CCWWGG 1 cut(s) 78
EcoT38I GRGCYC 2 cut(s) 438, 823
ErhI CCWWGG 1 cut(s) 78
Esp3I CGTCTC 1 cut(s) 500
FaeI CATG 3 cut(s) 259, 709, 922
FaiI YATR 4 cut(s) 257, 642, 707, 920
FaqI GGGAC 4 cut(s) 463, 497, 629, 893
FatI CATG 3 cut(s) 255, 705, 918
FauI CCCGC 2 cut(s) 75, 116
Fnu4HI GCNGC 5 cut(s) 139, 512, 956, 969, 1013
FokI GGATG 3 cut(s) 158, 737, 869
FriOI GRGCYC 2 cut(s) 438, 823
Fsp4HI GCNGC 5 cut(s) 139, 512, 956, 969, 1013
FspBI CTAG 1 cut(s) 561
GlaI GCGC 2 cut(s) 400, 870
GluI GCNGC 5 cut(s) 139, 512, 956, 969, 1013
GsaI CCCAGC 2 cut(s) 855, 900
GsuI CTGGAG 1 cut(s) 803
HaeII RGCGCY 1 cut(s) 872
HaeIII GGCC 3 cut(s) 710, 821, 901
HapII CCGG 3 cut(s) 163, 188, 439
HgaI GACGC 1 cut(s) 517
HhaI GCGC 2 cut(s) 401, 871
Hin1I GRCGYC 2 cut(s) 297, 410
Hin1II CATG 3 cut(s) 259, 709, 922
Hin6I GCGC 2 cut(s) 399, 869
HinP1I GCGC 2 cut(s) 399, 869
HinfI GANTC 6 cut(s) 20, 56, 126, 344, 646, 909
HpaII CCGG 3 cut(s) 163, 188, 439
HphI GGTGA 3 cut(s) 58, 353, 607
Hpy166II GTNNAC 2 cut(s) 169, 538
Hpy188I TCNGA 1 cut(s) 755
Hpy188III TCNNGA 2 cut(s) 505, 524
Hpy8I GTNNAC 2 cut(s) 169, 538
Hpy99I CGWCG 5 cut(s) 137, 299, 356, 506, 766
HpyAV CCTTC 3 cut(s) 190, 235, 1015
HpyCH4III ACNGT 2 cut(s) 352, 892
HpyCH4IV ACGT 3 cut(s) 50, 297, 410
HpyCH4V TGCA 5 cut(s) 474, 958, 971, 995, 1012
HpyF10VI GCNNNNNNNGC 7 cut(s) 22, 67, 250, 387, 396, 766, 787
HpyF3I CTNAG 3 cut(s) 301, 651, 864
HpySE526I ACGT 3 cut(s) 50, 297, 410
Hsp92I GRCGYC 2 cut(s) 297, 410
Hsp92II CATG 3 cut(s) 259, 709, 922
HspAI GCGC 2 cut(s) 399, 869
Kzo9I GATC 2 cut(s) 741, 755
LmnI GCTCC 3 cut(s) 406, 622, 778
Lsp1109I GCAGC 5 cut(s) 150, 523, 942, 955, 1024
LweI GCATC 4 cut(s) 34, 468, 756, 999
MaeI CTAG 1 cut(s) 561
MaeII ACGT 3 cut(s) 50, 297, 410
MaeIII GTNAC 2 cut(s) 46, 341
MalI GATC 2 cut(s) 743, 757
MboI GATC 2 cut(s) 741, 755
MhlI GDGCHC 3 cut(s) 438, 627, 823
MlsI TGGCCA 1 cut(s) 710
MluCI AATT 4 cut(s) 444, 477, 600, 714
MluNI TGGCCA 1 cut(s) 710
MlyI GAGTC 3 cut(s) 65, 338, 655
Mox20I TGGCCA 1 cut(s) 710
MscI TGGCCA 1 cut(s) 710
MseI TTAA 2 cut(s) 371, 948
MslI CAYNNNNRTG 1 cut(s) 923
Msp20I TGGCCA 1 cut(s) 710
MspA1I CMGCKG 4 cut(s) 70, 406, 514, 955
MspI CCGG 3 cut(s) 163, 188, 439
MspR9I CCNGG 4 cut(s) 164, 188, 365, 439
Mva1269I GAATGC 1 cut(s) 811
MvaI CCWGG 1 cut(s) 365
MvnI CGCG 1 cut(s) 548
MwoI GCNNNNNNNGC 7 cut(s) 22, 67, 250, 387, 396, 766, 787
NciI CCSGG 3 cut(s) 164, 188, 439
NdeII GATC 2 cut(s) 741, 755
NlaIII CATG 3 cut(s) 259, 709, 922
NlaIV GGNNCC 3 cut(s) 821, 822, 881
NmuCI GTSAC 2 cut(s) 46, 341
PaqCI CACCTGC 2 cut(s) 479, 926
PcsI WCGNNNNNNNCGW 1 cut(s) 423
PctI GAATGC 1 cut(s) 811
PfeI GAWTC 3 cut(s) 20, 126, 909
Pfl23II CGTACG 1 cut(s) 51
PkrI GCNGC 5 cut(s) 140, 513, 957, 970, 1014
Ple19I CGATCG 1 cut(s) 758
PleI GAGTC 3 cut(s) 64, 338, 654
PpsI GAGTC 3 cut(s) 64, 338, 654
PpuMI RGGWCCY 1 cut(s) 880
PshBI ATTAAT 1 cut(s) 948
Psp5II RGGWCCY 1 cut(s) 880
Psp6I CCWGG 1 cut(s) 363
PspFI CCCAGC 2 cut(s) 851, 896
PspGI CCWGG 1 cut(s) 363
PspLI CGTACG 1 cut(s) 51
PspN4I GGNNCC 3 cut(s) 821, 822, 881
PspOMI GGGCCC 1 cut(s) 819
PspPI GGNCC 4 cut(s) 819, 820, 880, 899
PspPPI RGGWCCY 1 cut(s) 880
PstI CTGCAG 1 cut(s) 960
PvuI CGATCG 1 cut(s) 758
PvuII CAGCTG 2 cut(s) 514, 955
RsaI GTAC 6 cut(s) 53, 74, 155, 539, 704, 929
RsaNI GTAC 6 cut(s) 52, 73, 154, 538, 703, 928
RseI CAYNNNNRTG 1 cut(s) 923
SaqAI TTAA 2 cut(s) 371, 948
SatI GCNGC 5 cut(s) 139, 512, 956, 969, 1013
Sau3AI GATC 2 cut(s) 741, 755
Sau96I GGNCC 4 cut(s) 819, 820, 880, 899
ScaI AGTACT 1 cut(s) 155
SchI GAGTC 3 cut(s) 65, 338, 655
ScrFI CCNGG 4 cut(s) 164, 188, 365, 439
SduI GDGCHC 3 cut(s) 438, 627, 823
SfaNI GCATC 4 cut(s) 34, 468, 756, 999
SfcI CTRYAG 1 cut(s) 956
SinI GGWCC 1 cut(s) 880
SmiMI CAYNNNNRTG 1 cut(s) 923
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 4 cut(s) 444, 477, 600, 714
SsiI CCGC 9 cut(s) 35, 68, 109, 242, 325, 404, 776, 933, 941
SspMI CTAG 1 cut(s) 561
StyD4I CCNGG 4 cut(s) 162, 186, 363, 437
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 2 cut(s) 352, 892
TaiI ACGT 3 cut(s) 53, 300, 413
TasI AATT 4 cut(s) 444, 477, 600, 714
TatI WGTACW 2 cut(s) 153, 927
TfiI GAWTC 3 cut(s) 20, 126, 909
Tru1I TTAA 2 cut(s) 371, 948
Tru9I TTAA 2 cut(s) 371, 948
TscAI CASTG 2 cut(s) 777, 965
TseFI GTSAC 2 cut(s) 46, 341
TseI GCWGC 5 cut(s) 138, 511, 955, 968, 1012
Tsp45I GTSAC 2 cut(s) 46, 341
TspRI CASTG 2 cut(s) 777, 965
VpaK11BI GGWCC 1 cut(s) 880
VspI ATTAAT 1 cut(s) 948
XagI CCTNNNNNAGG 1 cut(s) 332
XapI RAATTY 1 cut(s) 600
XcmI CCANNNNNNNNNTGG 1 cut(s) 718
XspI CTAG 1 cut(s) 561
ZraI GACGTC 2 cut(s) 298, 411
ZrmI AGTACT 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.