Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0006319

Physical Location & Seq

Genomic Coordinates Forward (+)

53575989 .. 53583584

Gene Structure 7596 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0437200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3294 bp

ATGCCTGGGCCCTACTTTAGGGTTTTAGCCAAGCCGCCAAGCTCTTACCAGTCCATCGGCAACCAAATCAAAGTTGCAGCGGCGCCTTATCTCCTCCGTCACTTCCATATCCGCCCTCCTCTTCTTCGATCAGCTCCGGCCGGCAAAGCCATGGACGCTGGAATCGAAGACCCGGAGAAGAAGAAGAAGAAGGAAGAGAAGGCAAGAGAGAAAGAATTGAAAAAGCAAAAAGCGTTAGAGAAAGCTGCCAAGTTCCAGGCACAACAAGCGAATGCTGCTTTGAAGAAGAGTGAGAAGAAGAGTGTGAAGCGTGGGAATGAAGAGGATGAAAATGCAGCTGATTTTGTTGATCCTCCGACTCCGGTTGGACAGAAGAAGCAGATGTCTAAGCAGATGGCCAAACAGTACAGTCCAAGTGCTGTCGAGAAGGGGTGGTATCAGTGGTGGGAGGAAGTGGGGTATTTTGTGCCAGATGCTGAGAGCTCTAAACCTCCGTTTGTGATTGTTTTGCCGCCTCCCAATGTGACTGGCGCGCTTCATATAGGCCATGCCCTTACTGCTGCTATTGAGGACACTATGATTCGTTGGCGAAGAATGTCGGGTTACAATGCCTTGTGGGTGCCTGGAATGGACCATGCTGGGATTGCAACTCAGGTTGTTGTAGAAAAGAAGCTGATGCGTGAACGCAATTTAACCCGACATGATATTGGTCGCGACGAGTTTGTGTCTGAAGTTTGGAAATGGAAAAATAATTACGGGGGCACTATACTACAGCAGCTACGTCGTCTGGGTGCTTCATTGGATTGGTCCCGTGAGTGCTTTACAATGGATGAGAAGAGGTCAAATGCTGTGACGGAGGCTTTTGTGAGGCTTCATAAGCAAGGGCTTATCTACAGGGATAACCGCCTAGTGAATTGGGATTGTGTCTTGCGAACGGCAATATCTGAAATTGAGGTGGACTATATAGATATAAAAGAAAGAACATCTCTTGAGGTTCCTGGCTACAAGAACCGTGTTGAATTTGGCGTTTTAACATCATTTGCATACCCTCTGGAAGACAATTTGGGTGAGATAGTTGTGGCAACCACCAGAGTGGAAACTATGCTCGGTGATACTGCTATTGCTGTACATCCAGATGATGACAGATATAAGCATCTTCATGGAAAGCATGCCATTCATCCATTTAATGGAAGAAGGATTCGTATAATCTGTGATGCAATTCTCGTTGATCCAGAGTTTGGGACTGGTGCTGTGAAGATTACTCCGGCCCATGATCCTAATGACTTTAACGTCGGAAAGCGCCATAATCTGGACTTTATAAATGTCTTCACTGATGATGGAAAAATAAACCAGGATGGTGGAGAGTTTGCAGGGATGCCACGCTTCAAAGCCCGGGAGGCAGTTACTGAAGCATTAAAGAAGAAGGGCCTCTTTAAAGAAGCAACGACAAATGAGATGCGTCTAGGTATATGCTCTAGAAGCAAGGATGTTGTTGAACCCATGATAAAGCCCCAGTGGTATGTCAAATGTAGTGGTATGGCCAAGGGAGCCCTTGATGCTGCTGTAGATGATGATAATAGAAAGCTTGACATCATTCCGAGACAGTATAATGCTGATTGGAAGAGATGGCTTGAGAACATTCGTGATTGGTGCGTCTCACGGCAACTTTGGTGGGGTCACCGCATTCCTGCATGGTATGCCGTTTTGGATGGTGACAAGCCAGAAGATTTTGGATCATTGGATGACCGATGGGTGGTTGCAAGAAATGAGGAAGAGGCTAAAGCACTGGCTGGGCAAAGATATGAGGGAAAGGAGGTTAAACTAGAACAGGATCCTGATGTGCTTGACACGTGGTTTTCTTCTGGTCTCTTCCCATTGTCCGTGTTGGGGTGGCCGGAAGATACAGAAGATTTGAGAGCTTTCTATCCAACTTCAGTTCTTGAAACTGGGCATGACATTCTCTTTTTCTGGGTCGCTCGTATGGTAATGCTTGGAAGTACATTGGGTGGTAATGTACCTTTCACAAAGGTGTATTTGCATCCAATGATTCGTGATGCGCATGGGCGTAAAATGTCCAAGTCCTTGGGGAATGTCATTGATCCTCTTGATGTGATCAATGGTATTTCACTGGAAGATCTACACAAGAAGCTTTCAGAGGGAAATTTGGACCCAAGGGAAATAGCTATAGCCAAAGAAGGCCAAAAAAAGGATTTTCCTGATGGTATTGCTGAATGTGGTTCTGATGCGCTACATTTTGCTCTGGTCTCATACACTGCTCAGTCTGATAAGATAAATCTGGATATCCAAAGGGTTGTCGGTTACCGGCAGTGGTGTAATAAACTTTGGAACGCCGTACGATTTGCTATGAGCAAACTTGGGGATGATTATGTTCCGCCGTCAACAGTAATCCCGGATGTGCTTCCATTTAGTTGCCGGTGGATACTCTCGGTCCTAAATAAAGCCATCTCCAAAACTATTTTGTCATTGGAATCATACGAGTTTTCAGATGCAGCCACTGCAGTGTATGCTTGGTGGCAGTACCAATTGTGTGATGTGTTCATTGAAGCCATAAAACCCTACTTTGCTGGAAACAATCCAAAGTTTGCATCTGAAAGGGGTTTTGCACAAGACACGCTCTGGTTGTGTCTGGATACTGGACTACGGCTGCTTCACCCATTTATGCCTTTCGTTACCGAAGAACTATGGCAGCGGCTCCCATCATCCAAGCATCACAATAGGGCAGCATCAATTATGATATGCGACTACCCATCCATTGTGGAGTGCTGGACAAATGAAAGGGTGGAGTCTGAAATGAGTCTTATTGACTCTATTGTTAGATCTCTCCGGTCACTGTCTCAAGAGAGTCGTGAAAGACGACCAGCTTTTGTGCTGTGTCGATCAATGTCAGATCGGGAGATAATATGTAGACAACAACTAGAGATTGAAACTCTTGCCAATCTTTTATCTCTGACGGTAATCAGTGAGAATGATGCTGCCCCAACTGGATGTGCTGTGTCTGTTGTAAACGAAAACCTATCTGTTTATCTGAAGGTTCTAGGATCTCGTTCTGCAGAAATAGATCTTGAAAAGATCCGGAAAAAGATGGAAGACATTAAATTGCAACAGGAGAAGATAATGAAGGTGATGAATGCTGCCGGTTACAAAGAAAAGGTCCCCGAGAAGATTCAAAAAGCTAATGCTGATAAACTTGAATCCCTCGAGAAGGAAAAACTGTCTCTTGAAGAGGCAAGCCAACATATGGAAGTGCAGATATCAAACAATAAACAAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0008150 GO:0008544 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009888 GO:0009913 GO:0009957 GO:0009987 GO:0010026 GO:0010033 GO:0030154 GO:0030855 GO:0032502 GO:0032879 GO:0032880 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045165 GO:0048856 GO:0048869 GO:0050789 GO:0050896 GO:0060429 GO:0065007 GO:0090558 GO:0097159 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1097

Amino Acids

124.68

Weight (kDa)

6.6

Isoelectric Point (pI)

45.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	145 - 766	4.7e-143	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g	PF09334	171 - 233	6.9e-07	tRNA synthetases class I (M)
tRNA-synt_1_2	PF13603	387 - 447	6e-07	Leucyl-tRNA synthetase, editing domain
Anticodon_1	PF08264	812 - 952	2.9e-33	Anticodon-binding domain of tRNA ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006319)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G24520 AT5G24520 AT5G24520 AT5G24520
fragaria_vesca	FvH4_7g32970 FvH4_7g32970
malus_domestica	MD01G1228700.v1.1 MD07G1299800.v1.1
prunus_persica	Prupe.2G319500_v2.0.a1 Prupe.2G319500_v2.0.a1 Prupe.2G319500_v2.0.a1
pyrus_communis	pycom01g23670 pycom07g27200
rosa_chinensis	RchiOBHm_Chr1g0382931
rosa_laevigata	RLG00000008438 RLG00000026148
rosa_multiflora	Rmu_co8253715.1_g000001 Rmu_sc0000698.1_g000059 Rmu_sc0018693.1_g000001 Rmu_sc0018693.1_g000002
rosa_roxburghii	Rroxscaffold_4G00277770
rosa_rugosa	Rorug01G0437200
rosa_samantha	Rh1AG462700 Rh1BG419600 Rh1DG451600 Rh5AG038100
rosa_wichuraiana	Rw1G040930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1319
AasI	GACNNNNNNGTC	1 cut(s)	1289
Acc16I	TGCGCA	1 cut(s)	2058
AccB1I	GGYRCC	2 cut(s)	82, 619
AccB7I	CCANNNNNTGG	4 cut(s)	1187, 1238, 1309, 3259
AccI	GTMKAC	1 cut(s)	2926
AccII	CGCG	2 cut(s)	533, 714
AccIII	TCCGGA	1 cut(s)	3093
AciI	CCGC	8 cut(s)	35, 80, 112, 512, 904, 1681, 2393, 2710
AclWI	GGATC	9 cut(s)	344, 1223, 1268, 1741, 1826, 1839, 2093, 3067, 3085
AcoI	YGGCCR	4 cut(s)	138, 396, 1539, 1892
AcsI	RAATTY	2 cut(s)	1019, 2161
AcuI	CTGAAG	4 cut(s)	750, 1428, 1917, 3068
AcvI	CACGTG	1 cut(s)	1851
AcyI	GRCGYC	1 cut(s)	83
AfaI	GTAC	6 cut(s)	407, 1128, 1999, 2016, 2355, 2540
AfiI	CCNNNNNNNGG	9 cut(s)	18, 1187, 1238, 1309, 1753, 1887, 2206, 3223, 3259
AflIII	ACRYGT	1 cut(s)	1848
AjnI	CCWGG	5 cut(s)	4, 255, 622, 997, 1350
AleI	CACNNNNGTG	3 cut(s)	1091, 2027, 2519
Alw21I	GWGCWC	1 cut(s)	485
Alw26I	GTCTC	6 cut(s)	1594, 1660, 1871, 2269, 2859, 3240
AlwI	GGATC	9 cut(s)	344, 1223, 1268, 1741, 1826, 1839, 2093, 3067, 3085
AlwNI	CAGNNNCTG	3 cut(s)	476, 1406, 2516
Ama87I	CYCGRG	3 cut(s)	1392, 3176, 3218
Aor13HI	TCCGGA	1 cut(s)	3093
AoxI	GGCC	9 cut(s)	8, 138, 396, 544, 1266, 1426, 1539, 1892, 2197
ApaI	GGGCCC	1 cut(s)	12
ApoI	RAATTY	2 cut(s)	1019, 2161
ArsI	GACNNNNNNTTYG	2 cut(s)	3156, 3188
AspLEI	GCGC	6 cut(s)	85, 533, 535, 1302, 2059, 2248
AspS9I	GGNCC	9 cut(s)	8, 9, 631, 807, 1267, 1426, 2167, 2449, 3172
AsuC2I	CCSGG	4 cut(s)	173, 1393, 1394, 2411
AsuHPI	GGTGA	6 cut(s)	1079, 1121, 1670, 1724, 2663, 3154
AvaI	CYCGRG	3 cut(s)	1392, 3176, 3218
AvaII	GGWCC	5 cut(s)	631, 807, 2167, 2449, 3172
BaeGI	GKGCMC	2 cut(s)	12, 764
BalI	TGGCCA	2 cut(s)	398, 1541
BamHI	GGATCC	1 cut(s)	1831
BanI	GGYRCC	2 cut(s)	82, 619
BanII	GRGCYC	3 cut(s)	12, 485, 1552
BbrPI	CACGTG	1 cut(s)	1851
BbsI	GAAGAC	4 cut(s)	174, 1062, 1318, 3114
Bbv12I	GWGCWC	1 cut(s)	485
BceAI	ACGGC	6 cut(s)	951, 1676, 1685, 2336, 2380, 2678
BcgI	CGANNNNNNTGC	2 cut(s)	764, 798
BciT130I	CCWGG	5 cut(s)	6, 257, 624, 999, 1352
BciVI	GTATCC	2 cut(s)	2433, 2644
BclI	TGATCA	1 cut(s)	2112
BcnI	CCSGG	4 cut(s)	173, 1393, 1394, 2411
BcoDI	GTCTC	6 cut(s)	1594, 1660, 1871, 2269, 2859, 3240
BfaI	CTAG	6 cut(s)	908, 1463, 1476, 1823, 2936, 3056
BfmI	CTRYAG	6 cut(s)	770, 892, 1563, 2184, 2517, 3069
BfoI	RGCGCY	2 cut(s)	86, 1303
BfuI	GTATCC	2 cut(s)	2433, 2644
BglI	GCCNNNNNGGC	1 cut(s)	1397
BglII	AGATCT	3 cut(s)	2134, 2837, 3079
Bme1390I	CCNGG	9 cut(s)	6, 173, 257, 624, 999, 1352, 1393, 1394, 2411
Bme18I	GGWCC	5 cut(s)	631, 807, 2167, 2449, 3172
BmeT110I	CYCGRG	3 cut(s)	1392, 3176, 3218
BmgT120I	GGNCC	9 cut(s)	8, 9, 631, 807, 1267, 1426, 2167, 2449, 3172
BmrFI	CCNGG	9 cut(s)	6, 173, 257, 624, 999, 1352, 1393, 1394, 2411
BmrI	ACTGGG	2 cut(s)	1507, 1956
BmuI	ACTGGG	2 cut(s)	1507, 1956
BpiI	GAAGAC	4 cut(s)	174, 1062, 1318, 3114
BpuEI	CTTGAG	3 cut(s)	1010, 1652, 2841
BpuMI	CCSGG	4 cut(s)	173, 1393, 1394, 2411
BsaAI	YACGTR	1 cut(s)	1851
BsaHI	GRCGYC	1 cut(s)	83
BsaI	GGTCTC	2 cut(s)	1871, 2269
BsaJI	CCNNGG	6 cut(s)	5, 150, 1392, 1542, 2082, 2171
BsaWI	WCCGGW	3 cut(s)	361, 2844, 3093
Bsc4I	CCNNNNNNNGG	9 cut(s)	18, 1187, 1238, 1309, 1753, 1887, 2206, 3223, 3259
Bse118I	RCCGGY	4 cut(s)	140, 2322, 2433, 3155
Bse1I	ACTGG	9 cut(s)	49, 532, 1249, 1513, 1791, 1951, 2133, 2659, 3007
BseAI	TCCGGA	1 cut(s)	3093
BseBI	CCWGG	5 cut(s)	6, 257, 624, 999, 1352
BseDI	CCNNGG	6 cut(s)	5, 150, 1392, 1542, 2082, 2171
BseLI	CCNNNNNNNGG	9 cut(s)	18, 1187, 1238, 1309, 1753, 1887, 2206, 3223, 3259
BseMII	CTCAG	3 cut(s)	468, 665, 2291
BseNI	ACTGG	9 cut(s)	49, 532, 1249, 1513, 1791, 1951, 2133, 2659, 3007
BsePI	GCGCGC	1 cut(s)	531
BseRI	GAGGAG	2 cut(s)	83, 108
BseSI	GKGCMC	2 cut(s)	12, 764
BseX3I	CGGCCG	1 cut(s)	138
BseYI	CCCAGC	2 cut(s)	638, 1790
BsgI	GTGCAG	1 cut(s)	3287
Bsh1236I	CGCG	2 cut(s)	533, 714
Bsh1285I	CGRYCG	1 cut(s)	141
BshFI	GGCC	9 cut(s)	10, 140, 398, 546, 1268, 1428, 1541, 1894, 2199
BshNI	GGYRCC	2 cut(s)	82, 619
BsiEI	CGRYCG	1 cut(s)	141
BsiHKAI	GWGCWC	1 cut(s)	485
BsiHKCI	CYCGRG	3 cut(s)	1392, 3176, 3218
BsiWI	CGTACG	1 cut(s)	2353
BslFI	GGGAC	3 cut(s)	793, 1255, 3158
BslI	CCNNNNNNNGG	9 cut(s)	18, 1187, 1238, 1309, 1753, 1887, 2206, 3223, 3259
BsmAI	GTCTC	6 cut(s)	1594, 1660, 1871, 2269, 2859, 3240
BsmBI	CGTCTC	1 cut(s)	1660
BsmFI	GGGAC	3 cut(s)	793, 1255, 3158
BsmI	GAATGC	3 cut(s)	277, 1683, 3154
BsnI	GGCC	9 cut(s)	10, 140, 398, 546, 1268, 1428, 1541, 1894, 2199
Bso31I	GGTCTC	2 cut(s)	1871, 2269
BsoBI	CYCGRG	3 cut(s)	1392, 3176, 3218
Bsp120I	GGGCCC	1 cut(s)	8
Bsp1286I	GDGCHC	4 cut(s)	12, 485, 764, 1552
Bsp13I	TCCGGA	1 cut(s)	3093
Bsp1407I	TGTACA	1 cut(s)	1126
Bsp19I	CCATGG	1 cut(s)	150
Bsp68I	TCGCGA	1 cut(s)	714
BspACI	CCGC	8 cut(s)	35, 80, 112, 512, 904, 1681, 2393, 2710
BspANI	GGCC	9 cut(s)	10, 140, 398, 546, 1268, 1428, 1541, 1894, 2199
BspCNI	CTCAG	3 cut(s)	469, 664, 2290
BspEI	TCCGGA	1 cut(s)	3093
BspFNI	CGCG	2 cut(s)	533, 714
BspMAI	CTGCAG	2 cut(s)	2521, 3073
BspPI	GGATC	9 cut(s)	344, 1223, 1268, 1741, 1826, 1839, 2093, 3067, 3085
BspT107I	GGYRCC	2 cut(s)	82, 619
BspTNI	GGTCTC	2 cut(s)	1871, 2269
BsrFI	RCCGGY	4 cut(s)	140, 2322, 2433, 3155
BsrGI	TGTACA	1 cut(s)	1126
BsrI	ACTGG	9 cut(s)	49, 532, 1249, 1513, 1791, 1951, 2133, 2659, 3007
BssAI	RCCGGY	4 cut(s)	140, 2322, 2433, 3155
BssECI	CCNNGG	6 cut(s)	5, 150, 1392, 1542, 2082, 2171
BssHII	GCGCGC	1 cut(s)	531
BssNI	GRCGYC	1 cut(s)	83
BssT1I	CCWWGG	4 cut(s)	150, 1542, 2082, 2171
Bst2UI	CCWGG	5 cut(s)	6, 257, 624, 999, 1352
Bst4CI	ACNGT	8 cut(s)	405, 410, 1013, 1605, 2404, 2853, 2974, 3234
BstACI	GRCGYC	1 cut(s)	83
BstAPI	GCANNNNNTGC	3 cut(s)	1697, 2516, 2525
BstAUI	TGTACA	1 cut(s)	1126
BstBAI	YACGTR	1 cut(s)	1851
BstC8I	GCNNGC	4 cut(s)	142, 533, 1170, 3250
BstDEI	CTNAG	4 cut(s)	387, 477, 651, 2277
BstDSI	CCRYGG	1 cut(s)	150
BstEII	GGTNACC	2 cut(s)	1676, 2318
BstENI	CCTNNNNNAGG	2 cut(s)	16, 3221
BstFNI	CGCG	2 cut(s)	533, 714
BstH2I	RGCGCY	2 cut(s)	86, 1303
BstHHI	GCGC	6 cut(s)	85, 533, 535, 1302, 2059, 2248
BstMAI	GTCTC	6 cut(s)	1594, 1660, 1871, 2269, 2859, 3240
BstMCI	CGRYCG	1 cut(s)	141
BstNI	CCWGG	5 cut(s)	6, 257, 624, 999, 1352
BstNSI	RCATGY	1 cut(s)	1172
BstPI	GGTNACC	2 cut(s)	1676, 2318
BstSCI	CCNGG	9 cut(s)	4, 171, 255, 622, 997, 1350, 1391, 1392, 2409
BstSFI	CTRYAG	6 cut(s)	770, 892, 1563, 2184, 2517, 3069
BstSLI	GKGCMC	2 cut(s)	12, 764
BstUI	CGCG	2 cut(s)	533, 714
BstV2I	GAAGAC	4 cut(s)	174, 1062, 1318, 3114
BstX2I	RGATCY	6 cut(s)	1831, 2134, 2837, 3059, 3079, 3090
BstXI	CCANNNNNNTGG	3 cut(s)	1093, 1358, 2083
BstYI	RGATCY	6 cut(s)	1831, 2134, 2837, 3059, 3079, 3090
BstZI	CGGCCG	1 cut(s)	138
BsuI	GTATCC	2 cut(s)	2433, 2644
BsuRI	GGCC	9 cut(s)	10, 140, 398, 546, 1268, 1428, 1541, 1894, 2199
BtgI	CCRYGG	1 cut(s)	150
BtsI	GCAGTG	4 cut(s)	2271, 2333, 2514, 2526
BtuMI	TCGCGA	1 cut(s)	714
Cac8I	GCNNGC	4 cut(s)	142, 533, 1170, 3250
CaiI	CAGNNNCTG	3 cut(s)	476, 1406, 2516
CfoI	GCGC	6 cut(s)	85, 533, 535, 1302, 2059, 2248
Cfr10I	RCCGGY	4 cut(s)	140, 2322, 2433, 3155
Cfr13I	GGNCC	9 cut(s)	8, 9, 631, 807, 1267, 1426, 2167, 2449, 3172
Cfr9I	CCCGGG	1 cut(s)	1392
CseI	GACGC	3 cut(s)	164, 1448, 1642
Csp6I	GTAC	6 cut(s)	406, 1127, 1998, 2015, 2354, 2539
CspCI	CAANNNNNGTGG	4 cut(s)	1513, 1548, 1652, 1687
CviQI	GTAC	6 cut(s)	406, 1127, 1998, 2015, 2354, 2539
DdeI	CTNAG	4 cut(s)	387, 477, 651, 2277
DinI	GGCGCC	1 cut(s)	84
DraI	TTTAAA	1 cut(s)	1435
DrdI	GACNNNNNNGTC	1 cut(s)	1289
DseDI	GACNNNNNNGTC	1 cut(s)	1289
EaeI	YGGCCR	4 cut(s)	138, 396, 1539, 1892
EagI	CGGCCG	1 cut(s)	138
EciI	GGCGGA	2 cut(s)	101, 2382
Ecl136II	GAGCTC	1 cut(s)	483
EclXI	CGGCCG	1 cut(s)	138
Eco130I	CCWWGG	4 cut(s)	150, 1542, 2082, 2171
Eco24I	GRGCYC	3 cut(s)	12, 485, 1552
Eco31I	GGTCTC	2 cut(s)	1871, 2269
Eco32I	GATATC	2 cut(s)	2302, 3273
Eco47I	GGWCC	5 cut(s)	631, 807, 2167, 2449, 3172
Eco52I	CGGCCG	1 cut(s)	138
Eco53kI	GAGCTC	1 cut(s)	483
Eco57I	CTGAAG	4 cut(s)	750, 1428, 1917, 3068
Eco72I	CACGTG	1 cut(s)	1851
Eco88I	CYCGRG	3 cut(s)	1392, 3176, 3218
Eco91I	GGTNACC	2 cut(s)	1676, 2318
EcoICRI	GAGCTC	1 cut(s)	483
EcoNI	CCTNNNNNAGG	2 cut(s)	16, 3221
EcoO109I	RGGNCCY	3 cut(s)	9, 1426, 3172
EcoO65I	GGTNACC	2 cut(s)	1676, 2318
EcoRII	CCWGG	5 cut(s)	4, 255, 622, 997, 1350
EcoRV	GATATC	2 cut(s)	2302, 3273
EcoT14I	CCWWGG	4 cut(s)	150, 1542, 2082, 2171
EcoT38I	GRGCYC	3 cut(s)	12, 485, 1552
EgeI	GGCGCC	1 cut(s)	84
EheI	GGCGCC	1 cut(s)	84
ErhI	CCWWGG	4 cut(s)	150, 1542, 2082, 2171
Esp3I	CGTCTC	1 cut(s)	1660
FalI	AAGNNNNNCTT	2 cut(s)	1415, 1447
FaqI	GGGAC	3 cut(s)	793, 1255, 3158
FauNDI	CATATG	1 cut(s)	3258
FbaI	TGATCA	1 cut(s)	2112
FblI	GTMKAC	1 cut(s)	2926
FriOI	GRGCYC	3 cut(s)	12, 485, 1552
FspAI	RTGCGCAY	1 cut(s)	2058
FspBI	CTAG	6 cut(s)	908, 1463, 1476, 1823, 2936, 3056
FspI	TGCGCA	1 cut(s)	2058
GlaI	GCGC	6 cut(s)	84, 532, 534, 1301, 2058, 2247
GsaI	CCCAGC	2 cut(s)	642, 1794
HaeII	RGCGCY	2 cut(s)	86, 1303
HaeIII	GGCC	9 cut(s)	10, 140, 398, 546, 1268, 1428, 1541, 1894, 2199
HgaI	GACGC	3 cut(s)	164, 1448, 1642
HhaI	GCGC	6 cut(s)	85, 533, 535, 1302, 2059, 2248
Hin1I	GRCGYC	1 cut(s)	83
Hin6I	GCGC	6 cut(s)	83, 531, 533, 1300, 2057, 2246
HinP1I	GCGC	6 cut(s)	83, 531, 533, 1300, 2057, 2246
HincII	GTYRAC	1 cut(s)	2400
HindII	GTYRAC	1 cut(s)	2400
HindIII	AAGCTT	2 cut(s)	1583, 2147
HphI	GGTGA	6 cut(s)	1079, 1121, 1670, 1724, 2663, 3154
Hpy166II	GTNNAC	5 cut(s)	683, 958, 2400, 2927, 3025
Hpy8I	GTNNAC	5 cut(s)	683, 958, 2400, 2927, 3025
Hpy99I	CGWCG	3 cut(s)	719, 786, 1295
HpyAV	CCTTC	9 cut(s)	184, 193, 421, 1188, 1417, 2189, 3043, 3133, 3217
HpyCH4III	ACNGT	8 cut(s)	405, 410, 1013, 1605, 2404, 2853, 2974, 3234
HpyCH4IV	ACGT	3 cut(s)	781, 1290, 1850
HpyF3I	CTNAG	4 cut(s)	387, 477, 651, 2277
HpySE526I	ACGT	3 cut(s)	781, 1290, 1850
Hsp92I	GRCGYC	1 cut(s)	83
HspAI	GCGC	6 cut(s)	83, 531, 533, 1300, 2057, 2246
KasI	GGCGCC	1 cut(s)	82
Kpn2I	TCCGGA	1 cut(s)	3093
KroI	GCCGGC	1 cut(s)	140
KroNI	GCCGGC	1 cut(s)	142
Ksp22I	TGATCA	1 cut(s)	2112
LmnI	GCTCC	3 cut(s)	139, 1547, 2718
MaeI	CTAG	6 cut(s)	908, 1463, 1476, 1823, 2936, 3056
MaeII	ACGT	3 cut(s)	781, 1290, 1850
MfeI	CAATTG	1 cut(s)	2543
MflI	RGATCY	6 cut(s)	1831, 2134, 2837, 3059, 3079, 3090
MhlI	GDGCHC	4 cut(s)	12, 485, 764, 1552
MlsI	TGGCCA	2 cut(s)	398, 1541
MluNI	TGGCCA	2 cut(s)	398, 1541
Mly113I	GGCGCC	1 cut(s)	83
MlyI	GAGTC	5 cut(s)	352, 2813, 2819, 2824, 2872
MmeI	TCCRAC	4 cut(s)	346, 380, 1273, 1952
Mox20I	TGGCCA	2 cut(s)	398, 1541
MroI	TCCGGA	1 cut(s)	3093
MroNI	GCCGGC	1 cut(s)	140
MscI	TGGCCA	2 cut(s)	398, 1541
MseI	TTAA	8 cut(s)	692, 1031, 1185, 1287, 1415, 1434, 1818, 3114
MslI	CAYNNNNRTG	5 cut(s)	1091, 1134, 1158, 2027, 2519
Msp20I	TGGCCA	2 cut(s)	398, 1541
MspA1I	CMGCKG	3 cut(s)	80, 338, 2710
MspR9I	CCNGG	9 cut(s)	6, 173, 257, 624, 999, 1352, 1393, 1394, 2411
MunI	CAATTG	1 cut(s)	2543
Mva1269I	GAATGC	3 cut(s)	277, 1683, 3154
MvaI	CCWGG	5 cut(s)	6, 257, 624, 999, 1352
MvnI	CGCG	2 cut(s)	533, 714
NaeI	GCCGGC	1 cut(s)	142
NarI	GGCGCC	1 cut(s)	83
NciI	CCSGG	4 cut(s)	173, 1393, 1394, 2411
NcoI	CCATGG	1 cut(s)	150
NdeI	CATATG	1 cut(s)	3258
NgoMIV	GCCGGC	1 cut(s)	140
NmuCI	GTSAC	6 cut(s)	98, 523, 850, 1676, 1712, 2847
NruI	TCGCGA	1 cut(s)	714
NsbI	TGCGCA	1 cut(s)	2058
NspI	RCATGY	1 cut(s)	1172
OliI	CACNNNNGTG	3 cut(s)	1091, 2027, 2519
PaeI	GCATGC	1 cut(s)	1172
PaeR7I	CTCGAG	1 cut(s)	3218
PauI	GCGCGC	1 cut(s)	531
PcsI	WCGNNNNNNNCGW	2 cut(s)	162, 2872
PctI	GAATGC	3 cut(s)	277, 1683, 3154
PdiI	GCCGGC	1 cut(s)	142
PfeI	GAWTC	7 cut(s)	162, 580, 1198, 2047, 2489, 3184, 3212
Pfl23II	CGTACG	1 cut(s)	2353
PflMI	CCANNNNNTGG	4 cut(s)	1187, 1238, 1309, 3259
PfoI	TCCNGGA	1 cut(s)	2409
PleI	GAGTC	5 cut(s)	352, 2812, 2819, 2823, 2871
PluTI	GGCGCC	1 cut(s)	86
PmaCI	CACGTG	1 cut(s)	1851
PmlI	CACGTG	1 cut(s)	1851
PpsI	GAGTC	5 cut(s)	352, 2812, 2819, 2823, 2871
Ppu21I	YACGTR	1 cut(s)	1851
PpuMI	RGGWCCY	1 cut(s)	3172
PsiI	TTATAA	1 cut(s)	1319
Psp124BI	GAGCTC	1 cut(s)	485
Psp5II	RGGWCCY	1 cut(s)	3172
Psp6I	CCWGG	5 cut(s)	4, 255, 622, 997, 1350
PspCI	CACGTG	1 cut(s)	1851
PspEI	GGTNACC	2 cut(s)	1676, 2318
PspFI	CCCAGC	2 cut(s)	638, 1790
PspGI	CCWGG	5 cut(s)	4, 255, 622, 997, 1350
PspLI	CGTACG	1 cut(s)	2353
PspOMI	GGGCCC	1 cut(s)	8
PspPI	GGNCC	9 cut(s)	8, 9, 631, 807, 1267, 1426, 2167, 2449, 3172
PspPPI	RGGWCCY	1 cut(s)	3172
PstI	CTGCAG	2 cut(s)	2521, 3073
PstNI	CAGNNNCTG	3 cut(s)	476, 1406, 2516
PsuI	RGATCY	6 cut(s)	1831, 2134, 2837, 3059, 3079, 3090
PteI	GCGCGC	1 cut(s)	531
PvuII	CAGCTG	1 cut(s)	338
RruI	TCGCGA	1 cut(s)	714
RsaI	GTAC	6 cut(s)	407, 1128, 1999, 2016, 2355, 2540
RsaNI	GTAC	6 cut(s)	406, 1127, 1998, 2015, 2354, 2539
RseI	CAYNNNNRTG	5 cut(s)	1091, 1134, 1158, 2027, 2519
SacI	GAGCTC	1 cut(s)	485
SaqAI	TTAA	8 cut(s)	692, 1031, 1185, 1287, 1415, 1434, 1818, 3114
Sau96I	GGNCC	9 cut(s)	8, 9, 631, 807, 1267, 1426, 2167, 2449, 3172
SchI	GAGTC	5 cut(s)	352, 2813, 2819, 2824, 2872
ScrFI	CCNGG	9 cut(s)	6, 173, 257, 624, 999, 1352, 1393, 1394, 2411
SduI	GDGCHC	4 cut(s)	12, 485, 764, 1552
SfcI	CTRYAG	6 cut(s)	770, 892, 1563, 2184, 2517, 3069
SfoI	GGCGCC	1 cut(s)	84
Sfr274I	CTCGAG	1 cut(s)	3218
SinI	GGWCC	5 cut(s)	631, 807, 2167, 2449, 3172
SlaI	CTCGAG	1 cut(s)	3218
SmaI	CCCGGG	1 cut(s)	1394
SmiMI	CAYNNNNRTG	5 cut(s)	1091, 1134, 1158, 2027, 2519
SmlI	CTYRAG	4 cut(s)	989, 1631, 2856, 3218
SmoI	CTYRAG	4 cut(s)	989, 1631, 2856, 3218
SphI	GCATGC	1 cut(s)	1172
SsiI	CCGC	8 cut(s)	35, 80, 112, 512, 904, 1681, 2393, 2710
SspDI	GGCGCC	1 cut(s)	82
SspMI	CTAG	6 cut(s)	908, 1463, 1476, 1823, 2936, 3056
SstI	GAGCTC	1 cut(s)	485
StyD4I	CCNGG	9 cut(s)	4, 171, 255, 622, 997, 1350, 1391, 1392, 2409
StyI	CCWWGG	4 cut(s)	150, 1542, 2082, 2171
TaaI	ACNGT	8 cut(s)	405, 410, 1013, 1605, 2404, 2853, 2974, 3234
TaiI	ACGT	3 cut(s)	784, 1293, 1853
TaqI	TCGA	5 cut(s)	127, 165, 423, 2896, 3219
TaqII	GACCGA	2 cut(s)	1761, 2437
TatI	WGTACW	3 cut(s)	405, 1126, 1997
TauI	GCSGC	4 cut(s)	37, 83, 514, 2713
TfiI	GAWTC	7 cut(s)	162, 580, 1198, 2047, 2489, 3184, 3212
Tru1I	TTAA	8 cut(s)	692, 1031, 1185, 1287, 1415, 1434, 1818, 3114
Tru9I	TTAA	8 cut(s)	692, 1031, 1185, 1287, 1415, 1434, 1818, 3114
TseFI	GTSAC	6 cut(s)	98, 523, 850, 1676, 1712, 2847
Tsp45I	GTSAC	6 cut(s)	98, 523, 850, 1676, 1712, 2847
TspGWI	ACGGA	4 cut(s)	86, 483, 869, 1870
TspMI	CCCGGG	1 cut(s)	1392
Van91I	CCANNNNNTGG	4 cut(s)	1187, 1238, 1309, 3259
VpaK11BI	GGWCC	5 cut(s)	631, 807, 2167, 2449, 3172
XagI	CCTNNNNNAGG	2 cut(s)	16, 3221
XapI	RAATTY	2 cut(s)	1019, 2161
XbaI	TCTAGA	1 cut(s)	1475
XceI	RCATGY	1 cut(s)	1172
XhoI	CTCGAG	1 cut(s)	3218
XmaI	CCCGGG	1 cut(s)	1392
XmiI	GTMKAC	1 cut(s)	2926
XspI	CTAG	6 cut(s)	908, 1463, 1476, 1823, 2936, 3056

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.