Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0006319

Physical Location & Seq

Genomic Coordinates Reverse (-)

30587090 .. 30588075

Gene Structure 986 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000008438

Sequence Viewer

CDS Protein Promoter (2k)

Length: 708 bp

ATGCCTTGGCTTAGACCAGCCCACACCTATACCTCGACACGGCATTCACTCCACAAAACCCCACAGATCGACTCACCCCGACTCTTTCTCCAACTCACTCCGACTCTCTCACTATCCGACTCATTCCACAGCCACCCTGACTCTCTGAACGTTGTCGTTTTGGAGTTAGTGACCAACTATTTTACATTTTCCTTGGTGAATTTTCAAGCGGCAAGTTTTCATGGTCGAAAAATTTCGCGGTCTCCAAAAGGTTATAGGCGCGCTTCCAATTCCCAAATTGTCAATGAAAGATGTGATTGGGTCTTGTTCTTCTCTCACTCCAACTCCCTCCACAAGTCCAACGACATTCTCGCCTCCTTCGGCGACTACCTTCGCCTCTGGGAGGTCAAAGACTCCTCTATTGACCGCCTCGAGCCCATCTTCGTCCTCAACAACTCCAAGACCAGCGAGTTCTGTGCGCCGCTGACGTCATTCGACTGGAACGAGATTGAGCCTCGGCGAATTGGAACCTCCAGCATCGACACCACCTGTACGATTTGGGACATCGAGAAGGGAGTCGTCGAGACGCAGCTGATCGCTCACGATAAGGAGGTTTACAACATTGCGTGGGGCGAAGCTAGGGTTTTCGCCTCCGTCTCCGCCGACGGGTCGGTGAGGATTATTGATCTCCAACTCAAATCTTCTCCGACTCGTACCCATCGACTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0008150 GO:0008544 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009888 GO:0009913 GO:0009957 GO:0009987 GO:0010026 GO:0010033 GO:0030154 GO:0030855 GO:0032502 GO:0032879 GO:0032880 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045165 GO:0048856 GO:0048869 GO:0050789 GO:0050896 GO:0060429 GO:0065007 GO:0090558 GO:0097159 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

236

Amino Acids

26.84

Weight (kDa)

7.16

Isoelectric Point (pI)

45.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	111 - 228	6.2e-06	CDC20/Fizzy WD40 domain
WD40_Prp19	PF24814	156 - 224	1.2e-06	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	157 - 227	6.9e-09	THOC3 beta-propeller domain
WD40_Gbeta	PF25391	166 - 224	1e-06	G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st	PF25173	166 - 226	7.3e-06	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	167 - 226	7.1e-08	WDR5 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0006319)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G24520 AT5G24520 AT5G24520 AT5G24520
fragaria_vesca	FvH4_7g32970 FvH4_7g32970
malus_domestica	MD01G1228700.v1.1 MD07G1299800.v1.1
prunus_persica	Prupe.2G319500_v2.0.a1 Prupe.2G319500_v2.0.a1 Prupe.2G319500_v2.0.a1
pyrus_communis	pycom01g23670 pycom07g27200
rosa_chinensis	RchiOBHm_Chr1g0382931
rosa_laevigata	RLG00000008438 RLG00000026148
rosa_multiflora	Rmu_co8253715.1_g000001 Rmu_sc0000698.1_g000059 Rmu_sc0018693.1_g000001 Rmu_sc0018693.1_g000002
rosa_roxburghii	Rroxscaffold_4G00277770
rosa_rugosa	Rorug01G0437200
rosa_samantha	Rh1AG462700 Rh1BG419600 Rh1DG451600 Rh5AG038100
rosa_wichuraiana	Rw1G040930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	470
AccII	CGCG	2 cut(s)	238, 261
AciI	CCGC	5 cut(s)	209, 238, 406, 461, 639
AclI	AACGTT	1 cut(s)	150
AcsI	RAATTY	2 cut(s)	199, 231
AcyI	GRCGYC	1 cut(s)	467
AfaI	GTAC	2 cut(s)	532, 694
AfiI	CCNNNNNNNGG	3 cut(s)	39, 382, 645
AgsI	TTSAA	1 cut(s)	206
AluBI	AGCT	2 cut(s)	571, 617
AluI	AGCT	2 cut(s)	571, 617
Alw26I	GTCTC	3 cut(s)	246, 557, 640
Ama87I	CYCGRG	1 cut(s)	410
ApeKI	GCWGC	1 cut(s)	568
ApoI	RAATTY	2 cut(s)	199, 231
Asp700I	GAANNNNTTC	1 cut(s)	232
AspLEI	GCGC	3 cut(s)	261, 263, 460
AsuHPI	GGTGA	3 cut(s)	66, 208, 664
AvaI	CYCGRG	1 cut(s)	410
BanII	GRGCYC	1 cut(s)	417
BbvI	GCAGC	1 cut(s)	580
BccI	CCATC	2 cut(s)	425, 705
BceAI	ACGGC	1 cut(s)	56
BcgI	CGANNNNNNTGC	2 cut(s)	437, 471
BcoDI	GTCTC	3 cut(s)	246, 557, 640
BfaI	CTAG	1 cut(s)	618
BisI	GCNGC	3 cut(s)	210, 461, 569
BlsI	GCNGC	3 cut(s)	211, 462, 570
BmeT110I	CYCGRG	1 cut(s)	410
BmiI	GGNNCC	1 cut(s)	508
BmsI	GCATC	1 cut(s)	525
BpmI	CTGGAG	1 cut(s)	496
BsaHI	GRCGYC	1 cut(s)	467
BsaI	GGTCTC	1 cut(s)	246
BsaJI	CCNNGG	3 cut(s)	5, 192, 494
BsaXI	ACNNNNNCTCC	4 cut(s)	72, 102, 308, 338
Bsc4I	CCNNNNNNNGG	3 cut(s)	39, 382, 645
Bse1I	ACTGG	1 cut(s)	482
Bse3DI	GCAATG	1 cut(s)	600
BseDI	CCNNGG	3 cut(s)	5, 192, 494
BseLI	CCNNNNNNNGG	3 cut(s)	39, 382, 645
BseMI	GCAATG	1 cut(s)	600
BseNI	ACTGG	1 cut(s)	482
BsePI	GCGCGC	1 cut(s)	259
BseRI	GAGGAG	1 cut(s)	385
BseXI	GCAGC	1 cut(s)	580
Bsh1236I	CGCG	2 cut(s)	238, 261
BsiHKCI	CYCGRG	1 cut(s)	410
BslFI	GGGAC	1 cut(s)	554
BslI	CCNNNNNNNGG	3 cut(s)	39, 382, 645
BsmAI	GTCTC	3 cut(s)	246, 557, 640
BsmBI	CGTCTC	2 cut(s)	557, 640
BsmFI	GGGAC	1 cut(s)	554
BsmI	GAATGC	1 cut(s)	43
Bso31I	GGTCTC	1 cut(s)	246
BsoBI	CYCGRG	1 cut(s)	410
Bsp1286I	GDGCHC	1 cut(s)	417
Bsp143I	GATC	3 cut(s)	66, 573, 664
BspACI	CCGC	5 cut(s)	209, 238, 406, 461, 639
BspFNI	CGCG	2 cut(s)	238, 261
BspLI	GGNNCC	1 cut(s)	508
BspTNI	GGTCTC	1 cut(s)	246
BsrDI	GCAATG	1 cut(s)	600
BsrI	ACTGG	1 cut(s)	482
BssECI	CCNNGG	3 cut(s)	5, 192, 494
BssHII	GCGCGC	1 cut(s)	259
BssMI	GATC	3 cut(s)	66, 573, 664
BssNI	GRCGYC	1 cut(s)	467
BssT1I	CCWWGG	2 cut(s)	5, 192
BstACI	GRCGYC	1 cut(s)	467
BstC8I	GCNNGC	1 cut(s)	261
BstDEI	CTNAG	1 cut(s)	11
BstENI	CCTNNNNNAGG	1 cut(s)	380
BstFNI	CGCG	2 cut(s)	238, 261
BstHHI	GCGC	3 cut(s)	261, 263, 460
BstKTI	GATC	3 cut(s)	69, 576, 667
BstMAI	GTCTC	3 cut(s)	246, 557, 640
BstMBI	GATC	3 cut(s)	66, 573, 664
BstUI	CGCG	2 cut(s)	238, 261
BstV1I	GCAGC	1 cut(s)	580
Cac8I	GCNNGC	1 cut(s)	261
CfoI	GCGC	3 cut(s)	261, 263, 460
CseI	GACGC	1 cut(s)	574
Csp6I	GTAC	2 cut(s)	531, 693
CviAII	CATG	1 cut(s)	221
CviJI	RGCY	7 cut(s)	10, 20, 132, 415, 493, 571, 617
CviKI_1	RGCY	7 cut(s)	10, 20, 132, 415, 493, 571, 617
CviQI	GTAC	2 cut(s)	531, 693
DdeI	CTNAG	1 cut(s)	11
DpnI	GATC	3 cut(s)	68, 575, 666
DpnII	GATC	3 cut(s)	66, 573, 664
EciI	GGCGGA	1 cut(s)	628
Eco130I	CCWWGG	2 cut(s)	5, 192
Eco24I	GRGCYC	1 cut(s)	417
Eco31I	GGTCTC	1 cut(s)	246
Eco88I	CYCGRG	1 cut(s)	410
EcoNI	CCTNNNNNAGG	1 cut(s)	380
EcoT14I	CCWWGG	2 cut(s)	5, 192
EcoT38I	GRGCYC	1 cut(s)	417
ErhI	CCWWGG	2 cut(s)	5, 192
Esp3I	CGTCTC	2 cut(s)	557, 640
FaeI	CATG	1 cut(s)	224
FaiI	YATR	3 cut(s)	30, 222, 255
FaqI	GGGAC	1 cut(s)	554
FatI	CATG	1 cut(s)	220
Fnu4HI	GCNGC	3 cut(s)	210, 461, 569
FriOI	GRGCYC	1 cut(s)	417
Fsp4HI	GCNGC	3 cut(s)	210, 461, 569
FspBI	CTAG	1 cut(s)	618
GlaI	GCGC	3 cut(s)	260, 262, 459
GluI	GCNGC	3 cut(s)	210, 461, 569
GsuI	CTGGAG	1 cut(s)	496
HgaI	GACGC	1 cut(s)	574
HhaI	GCGC	3 cut(s)	261, 263, 460
Hin1I	GRCGYC	1 cut(s)	467
Hin1II	CATG	1 cut(s)	224
Hin6I	GCGC	3 cut(s)	259, 261, 458
HinP1I	GCGC	3 cut(s)	259, 261, 458
HinfI	GANTC	9 cut(s)	71, 81, 103, 119, 140, 392, 555, 688, 702
HphI	GGTGA	3 cut(s)	66, 208, 664
Hpy166II	GTNNAC	1 cut(s)	595
Hpy188I	TCNGA	5 cut(s)	102, 118, 147, 687, 707
Hpy188III	TCNNGA	3 cut(s)	547, 562, 581
Hpy8I	GTNNAC	1 cut(s)	595
Hpy99I	CGWCG	2 cut(s)	563, 647
HpyAV	CCTTC	3 cut(s)	367, 380, 544
HpyCH4IV	ACGT	2 cut(s)	150, 467
HpyF3I	CTNAG	1 cut(s)	11
HpySE526I	ACGT	2 cut(s)	150, 467
Hsp92I	GRCGYC	1 cut(s)	467
Hsp92II	CATG	1 cut(s)	224
HspAI	GCGC	3 cut(s)	259, 261, 458
Kzo9I	GATC	3 cut(s)	66, 573, 664
LpnPI	CCDG	7 cut(s)	30, 150, 364, 457, 463, 526, 541
Lsp1109I	GCAGC	1 cut(s)	580
LweI	GCATC	1 cut(s)	525
MaeI	CTAG	1 cut(s)	618
MaeII	ACGT	2 cut(s)	150, 467
MaeIII	GTNAC	1 cut(s)	169
MalI	GATC	3 cut(s)	68, 575, 666
MboI	GATC	3 cut(s)	66, 573, 664
MboII	GAAGA	3 cut(s)	301, 412, 672
MhlI	GDGCHC	1 cut(s)	417
MluCI	AATT	5 cut(s)	199, 231, 268, 276, 501
MlyI	GAGTC	9 cut(s)	65, 75, 97, 113, 134, 386, 564, 682, 696
MmeI	TCCRAC	6 cut(s)	115, 125, 141, 345, 363, 694
MroXI	GAANNNNTTC	1 cut(s)	232
MspA1I	CMGCKG	2 cut(s)	463, 571
Mva1269I	GAATGC	1 cut(s)	43
MvnI	CGCG	2 cut(s)	238, 261
NdeII	GATC	3 cut(s)	66, 573, 664
NlaIII	CATG	1 cut(s)	224
NlaIV	GGNNCC	1 cut(s)	508
NmeAIII	GCCGAG	1 cut(s)	475
NmuCI	GTSAC	1 cut(s)	169
PaeR7I	CTCGAG	1 cut(s)	410
PauI	GCGCGC	1 cut(s)	259
PcsI	WCGNNNNNNNCGW	2 cut(s)	480, 697
PctI	GAATGC	1 cut(s)	43
PdmI	GAANNNNTTC	1 cut(s)	232
PkrI	GCNGC	3 cut(s)	211, 462, 570
PleI	GAGTC	9 cut(s)	65, 75, 97, 113, 134, 386, 563, 682, 696
PpsI	GAGTC	9 cut(s)	65, 75, 97, 113, 134, 386, 563, 682, 696
Psp1406I	AACGTT	1 cut(s)	150
PspN4I	GGNNCC	1 cut(s)	508
PspXI	VCTCGAGB	1 cut(s)	410
PteI	GCGCGC	1 cut(s)	259
PvuII	CAGCTG	1 cut(s)	571
RsaI	GTAC	2 cut(s)	532, 694
RsaNI	GTAC	2 cut(s)	531, 693
SatI	GCNGC	3 cut(s)	210, 461, 569
Sau3AI	GATC	3 cut(s)	66, 573, 664
SchI	GAGTC	9 cut(s)	65, 75, 97, 113, 134, 386, 564, 682, 696
SduI	GDGCHC	1 cut(s)	417
SfaNI	GCATC	1 cut(s)	525
Sfr274I	CTCGAG	1 cut(s)	410
SlaI	CTCGAG	1 cut(s)	410
SmlI	CTYRAG	1 cut(s)	410
SmoI	CTYRAG	1 cut(s)	410
Sse9I	AATT	5 cut(s)	199, 231, 268, 276, 501
SsiI	CCGC	5 cut(s)	209, 238, 406, 461, 639
SspMI	CTAG	1 cut(s)	618
StyI	CCWWGG	2 cut(s)	5, 192
TaiI	ACGT	2 cut(s)	153, 470
TaqI	TCGA	9 cut(s)	35, 69, 226, 411, 474, 519, 546, 561, 700
TasI	AATT	5 cut(s)	199, 231, 268, 276, 501
TauI	GCSGC	2 cut(s)	212, 463
TseFI	GTSAC	1 cut(s)	169
TseI	GCWGC	1 cut(s)	568
Tsp45I	GTSAC	1 cut(s)	169
TspDTI	ATGAA	2 cut(s)	209, 300
TspGWI	ACGGA	1 cut(s)	622
XagI	CCTNNNNNAGG	1 cut(s)	380
XapI	RAATTY	2 cut(s)	199, 231
XhoI	CTCGAG	1 cut(s)	410
XmnI	GAANNNNTTC	1 cut(s)	232
XspI	CTAG	1 cut(s)	618
ZraI	GACGTC	1 cut(s)	468

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.