Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0001237

Physical Location & Seq

Genomic Coordinates Reverse (-)

981104 .. 987990

Gene Structure 6887 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1006900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2898 bp

ATGCGTCAGCTGGCTGGTAACCTCTTGCTCTTTGTTTGCTTCATTATTATTGCATTGAGTTGCTTCACACTTCGGGGACTTGCTCAGCTGCCTAAGTTACCCCAACAGGAAGTTGATGCTCTCCAAGAAATTGCTACCACGATGGGTGCAAATTATTGGACTTTTAACACCGATGCTTGCCGAATTGAAACCGTGGGTCTAACAAAGAAGCAGCCGAAGGGATCCCAGGCAAATACTGATTGTGATTGCAACTTTGAGAACAGCACTGTTTGCCATGTTGCAACGCTAACGCTCAAGGGTTTCAGTTTACCTGGGTTGCTTCCACCTCAACTCGTCAAACTTCCTTACCTTCGAAAAATTGATTTTGCTTACAACTATCTCAATGGTACAATACCCCGGGAATGGGCTTCAACGAAACTAACTTATATCTCTGTTCTTGTCAATCGGTTATCGGGGCAAATCCCAAAGCATTTGGGACATATTACCACTCTGACATTCCTGAACCTTGAGGGGAACCAATTTTCTGGTATTGTTCCTACTGAGCTTGGGAATTTGGTTAATTTGCAGACTTTGATGCTTTCCTCCAATCAGCTGACTGGATACTTGCCAGAGACGTTTTCTGGGCTAAGGATTTTAAATGATTTCCGAATAAATGATAACAACTTTAGTGGAACACTACCTGATTGGGTACAGAATTGGAAAAAACTCACAAGATTGGAAATGCATTCGAGTGGACTGGAAGGACTGATTCCATCTAATATTTCTCTCTTGAACAATTTGGAAGAGTTGAGGATTAGTGACACAAATGGACCATATCAGGAATTTCCTTTGCTGAGAAATTTGACAAGCCTAGTAAGATTGGTTTTGAGGAACTGCAATATTTCCGGAGAAATCCCCGCATATATCTGGTCCATGAAGAATCTAGAGATGTTGGATGTCAGTTTCAACAAGTTAGCAGGCCAACTTCCATCCACGATAGGAGCTGAGCGTCTAAAATTTGTCTTTTTGACTGGCAACCTGCTAAGTGGAAACGTGCCAAGATCAATCTTGCGAGATGGAAGTATTGTTGATTTGTCCTACAATAACTTCACATTTCAAGGCCCTGAGAAACCGGATTGTCAAAAGAACTTGAATTTAAATCTAAACTTGTATCGGAGCTCTTCAGAGGAGAACAACTTAAGGGGAATTCTTCCATGCTTGAAGAATTTTAAGTGCCCACGATATTCAAACTGTATGCATATTAATTGTGGTGGGAGTGACATAATTGTCAAGGACGAGAATAACGCGAACGTGTTGTTTGAAGGAGATGGAAGAGTTGAAGGTGGCAGTGCCAAGTATTTTAGGAATGAGAAAAGTATGTGGGGATTTAGTAGCACCGGAGACTTCATGGATGGCTTCGAGCTGCAGAATACACGCTACTCTGTATCTCTACCATCTTCCAATCTTTCTGAGCTATATACAACAGCACGCATATCTCCGCTTTCACTTACTTATTTCCACTATTGTTTAGAAAATGGGATTTACACTATAACTCTCCACTTTGCGGAGATAAAATTCACTAATGACAAGGGATACACTAGTCTTGGGAGGCGTATTTTCGATATCTATGTTCAGGAAAAGTTGGTGTGGAAGGATTTCAATATTGAACATGAGGCAGGCATGGCTCAAAAGAGATTAGTTAAGCAAGTAGATAATGTCAATGTGACAAGTAACGTCTTAGAGATCCGGTTCTACTGGGCTGGAAAAGGGACAACAAGGATTCCGGAGAGAGGACACTATGGTCCCCTCATATCAGCTGTCTCTGTGGTTTCCAACTTTAAACCGTGTACAAATAGTGGAACTGTGCGAACCATTTACATCATTGCCGGTGTTGTTGGGGGAATCTGCCTAACACTATTCATAGTAGCAATCCTTTGGTGGAAAAGATGTTTGCGAGGAAAAAGGGGGAGACAAAAAGATCTTCTTGGACGAGATATGCAGATGAGGACGTTTACTTTCAAACAAATAAAAGCTGCTACTAATGACTTTGATTCTGCTAAAAAGATTGGAGAAGGTGGCTTTGGTCCTGTCTACAAGGGTCACTTATCCGATGGTTCATTGATAGCAGTTAAGCAGCTCTCGTCCAATTCAAAGCAAGGAAATCGCGAATTTTTAAATGAGATGGGCATGATAACTTGTTTGCAACACCCAAATCTAGTGACACTGCATGGATGCTGTATTGAAAGGGATCAATTATTACTGGTGTACGAATACATGGAAAACAATAGTCTTGCGAATGCCTTATTTGGCCCGGAGAACCGGATAAACCTGGACTGGCCTACAAGGGTTAAGATCTGCACCGGGATAGCAAGAGGTTTAGCCTTTCTGCATGAAGAATCAAGACTCAAGATTGTTCACAGAGACATAAAAGCCACCAATGTGCTGCTGGATGGGGATTTGAACCCTAAAATATCGGACTTTGGATTGGCTAAACTTTATGATGAAGAGAAAACACACGTCAGCACCAAAGTTGCAGGAACCATAGGATATATGGCCCCAGAATATGCGCTATTGGGTCACTTAACCTACAAGGCAGATGTTTACAGTTTTGGAGTTGTTGCGTTGGAAATTGTTAGTGGGAAGAAGAACAGTTATGTGCCAAGTAACGCTTGTGTCTCTCTCCTTGACTGGGCCTATCAGTTGCAACAAGGTGGAAACTTGAAGGAGCTGATAGATGAGAGCTTAGTGAGTGAAATCAATGGCAAAGAAGCAGAAGTAATGGTTAAAGTCGGTTTGCTGTGCACGAATGTTTCTCCGTCACTTAGGCCTACCATGTCCGAGGTGGTGAGCATGCTTGAAGGACAAACAACAGTCCCGGACACGAAGCCAGAAGCGAGATATCATGCACTGAAGATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_8 Malectin Pkinase_Tyr

Protein Analysis

966

Amino Acids

107.86

Weight (kDa)

8.44

Isoelectric Point (pI)

32.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	155 - 265	2.1e-10	Leucine-rich repeat region
LRR_8	PF13855	163 - 222	1e-06	Leucine rich repeat
Malectin	PF11721	411 - 600	2.7e-40	Malectin domain
Pkinase	PF00069	677 - 942	1.3e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	679 - 945	1.6e-49	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001237)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29750 AT1G29750 AT1G29750
fragaria_vesca	FvH4_3g07010 FvH4_3g07010 FvH4_3g07045 FvH4_3g07045 FvH4_3g07045 FvH4_3g07045 FvH4_3g07045 FvH4_3g07045
malus_domestica	MD00G1006700.v1.1 MD00G1006800.v1.1 MD00G1006900.v1.1 MD00G1007000.v1.1 MD10G1273400.v1.1 MD10G1273600.v1.1
prunus_persica	Prupe.4G069100_v2.0.a1 Prupe.4G069200_v2.0.a1 Prupe.4G069200_v2.0.a1 Prupe.4G069200_v2.0.a1 Prupe.4G069200_v2.0.a1 Prupe.4G069200_v2.0.a1 Prupe.4G069200_v2.0.a1 Prupe.4G069200_v2.0.a1 Prupe.4G069200_v2.0.a1
pyrus_communis	pycom05g27170 pycom05g27180 pycom05g27210 pycom05g27220 pycom10g22820 pycom10g22840
rosa_chinensis	RchiOBHm_Chr5g0012071 RchiOBHm_Chr5g0012081 RchiOBHm_Chr5g0012091 RchiOBHm_Chr5g0012101
rosa_laevigata	RLG00000031884 RLG00000031885 RLG00000031889
rosa_multiflora	Rmu_sc0002519.1_g000010 Rmu_sc0002519.1_g000013 Rmu_sc0008172.1_g000001 Rmu_sc0009761.1_g000005
rosa_roxburghii	Rroxscaffold_1G00064490 Rroxscaffold_1G00064500 Rroxscaffold_1G00064510
rosa_rugosa	Rorug05G0000100 Rorug05G0000200 Rorug05G0000200
rosa_samantha	Rh5AG092900 Rh5BG089200 Rh5CG102700
rosa_wichuraiana	Rw5G008110 Rw5G008120 Rw5G008140 Rw5G008150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1265, 1779
Acc36I	ACCTGC	1 cut(s)	1026
AccI	GTMKAC	1 cut(s)	2070
AccII	CGCG	2 cut(s)	1286, 2145
AccIII	TCCGGA	2 cut(s)	884, 1762
AciI	CCGC	3 cut(s)	897, 1478, 1544
AclWI	GGATC	4 cut(s)	216, 229, 1717, 2235
AcsI	RAATTY	9 cut(s)	550, 821, 838, 995, 1132, 1185, 1204, 1553, 2147
AcuI	CTGAAG	1 cut(s)	1146
AfaI	GTAC	4 cut(s)	388, 690, 1828, 2246
AfiI	CCNNNNNNNGG	5 cut(s)	402, 403, 1543, 1769, 2668
AflII	CTTAAG	1 cut(s)	1177
AflIII	ACRYGT	2 cut(s)	1290, 2494
AhlI	ACTAGT	1 cut(s)	1577
AjiI	CACGTC	1 cut(s)	2497
AjnI	CCWGG	3 cut(s)	225, 310, 2307
AjuI	GAANNNNNNNTTGG	2 cut(s)	2043, 2075
AleI	CACNNNNGTG	1 cut(s)	2417
Alw21I	GWGCWC	2 cut(s)	1160, 2783
Alw26I	GTCTC	6 cut(s)	605, 1374, 1804, 1942, 2394, 2659
Alw44I	GTGCAC	1 cut(s)	2779
AlwI	GGATC	4 cut(s)	216, 229, 1717, 2235
Ama87I	CYCGRG	1 cut(s)	396
Aor13HI	TCCGGA	2 cut(s)	884, 1762
AoxI	GGCC	7 cut(s)	958, 1099, 2287, 2315, 2532, 2670, 2804
ApaLI	GTGCAC	1 cut(s)	2779
ApeKI	GCWGC	6 cut(s)	88, 211, 1402, 2012, 2113, 2422
ApoI	RAATTY	9 cut(s)	550, 821, 838, 995, 1132, 1185, 1204, 1553, 2147
AseI	ATTAAT	1 cut(s)	1242
Asp700I	GAANNNNTTC	1 cut(s)	1634
AspLEI	GCGC	1 cut(s)	2548
AspS9I	GGNCC	8 cut(s)	809, 909, 1100, 1781, 2063, 2288, 2533, 2670
AsuC2I	CCSGG	5 cut(s)	397, 398, 2291, 2341, 2855
AsuHPI	GGTGA	1 cut(s)	2836
AsuII	TTCGAA	1 cut(s)	352
AvaI	CYCGRG	1 cut(s)	396
AvaII	GGWCC	4 cut(s)	809, 909, 1781, 2063
BaeGI	GKGCMC	2 cut(s)	1217, 2783
BaeI	ACNNNNGTAYC	2 cut(s)	1564, 1597
BamHI	GGATCC	1 cut(s)	221
BanII	GRGCYC	1 cut(s)	1160
Bbv12I	GWGCWC	2 cut(s)	1160, 2783
BbvI	GCAGC	6 cut(s)	75, 223, 1389, 1999, 2125, 2409
BcgI	CGANNNNNNTGC	2 cut(s)	2123, 2157
BciT130I	CCWGG	3 cut(s)	227, 312, 2309
BciVI	GTATCC	2 cut(s)	593, 1565
BcnI	CCSGG	5 cut(s)	397, 398, 2291, 2341, 2855
BcoDI	GTCTC	6 cut(s)	605, 1374, 1804, 1942, 2394, 2659
BcuI	ACTAGT	1 cut(s)	1577
BfaI	CTAG	4 cut(s)	851, 923, 1578, 2195
BfmI	CTRYAG	1 cut(s)	1403
BfrI	CTTAAG	1 cut(s)	1177
BfuAI	ACCTGC	1 cut(s)	1026
BfuI	GTATCC	2 cut(s)	593, 1565
BglII	AGATCT	2 cut(s)	1957, 2331
BisI	GCNGC	6 cut(s)	89, 212, 1403, 2013, 2114, 2423
BlpI	GCTNAGC	2 cut(s)	84, 984
BlsI	GCNGC	6 cut(s)	90, 213, 1404, 2014, 2115, 2424
Bme1390I	CCNGG	8 cut(s)	227, 312, 397, 398, 2291, 2309, 2341, 2855
Bme18I	GGWCC	4 cut(s)	809, 909, 1781, 2063
BmeT110I	CYCGRG	1 cut(s)	396
BmgBI	CACGTC	1 cut(s)	2497
BmgT120I	GGNCC	8 cut(s)	809, 909, 1100, 1781, 2063, 2288, 2533, 2670
BmiI	GGNNCC	5 cut(s)	223, 515, 1783, 2518, 2535
BmrFI	CCNGG	8 cut(s)	227, 312, 397, 398, 2291, 2309, 2341, 2855
BmrI	ACTGGG	2 cut(s)	1744, 2677
BmsI	GCATC	4 cut(s)	106, 163, 564, 2201
BmuI	ACTGGG	2 cut(s)	1744, 2677
Bpu10I	CCTNAGC	1 cut(s)	626
Bpu1102I	GCTNAGC	2 cut(s)	84, 984
Bpu14I	TTCGAA	1 cut(s)	352
BpuEI	CTTGAG	3 cut(s)	278, 527, 2369
BpuMI	CCSGG	5 cut(s)	397, 398, 2291, 2341, 2855
BsaJI	CCNNGG	6 cut(s)	192, 225, 311, 395, 396, 2817
BsaWI	WCCGGW	6 cut(s)	884, 1111, 1376, 1725, 1762, 2298
Bsc4I	CCNNNNNNNGG	5 cut(s)	402, 403, 1543, 1769, 2668
Bse118I	RCCGGY	1 cut(s)	1865
Bse1I	ACTGG	7 cut(s)	601, 741, 1015, 1739, 2244, 2318, 2672
Bse3DI	GCAATG	1 cut(s)	1860
BseAI	TCCGGA	2 cut(s)	884, 1762
BseBI	CCWGG	3 cut(s)	227, 312, 2309
BseDI	CCNNGG	6 cut(s)	192, 225, 311, 395, 396, 2817
BseGI	GGATG	5 cut(s)	940, 968, 1396, 2216, 2434
BseLI	CCNNNNNNNGG	5 cut(s)	402, 403, 1543, 1769, 2668
BseMI	GCAATG	1 cut(s)	1860
BseMII	CTCAG	6 cut(s)	98, 531, 824, 975, 1095, 1440
BseNI	ACTGG	7 cut(s)	601, 741, 1015, 1739, 2244, 2318, 2672
BseRI	GAGGAG	1 cut(s)	1181
BseSI	GKGCMC	2 cut(s)	1217, 2783
BseXI	GCAGC	6 cut(s)	75, 223, 1389, 1999, 2125, 2409
BsgI	GTGCAG	1 cut(s)	2320
Bsh1236I	CGCG	2 cut(s)	1286, 2145
BshFI	GGCC	7 cut(s)	960, 1101, 2289, 2317, 2534, 2672, 2806
BsiHKAI	GWGCWC	2 cut(s)	1160, 2783
BsiHKCI	CYCGRG	1 cut(s)	396
BslFI	GGGAC	5 cut(s)	90, 489, 1762, 1767, 2837
BslI	CCNNNNNNNGG	5 cut(s)	402, 403, 1543, 1769, 2668
BsmAI	GTCTC	6 cut(s)	605, 1374, 1804, 1942, 2394, 2659
BsmBI	CGTCTC	1 cut(s)	605
BsmFI	GGGAC	5 cut(s)	90, 489, 1762, 1767, 2837
BsmI	GAATGC	2 cut(s)	724, 2281
BsnI	GGCC	7 cut(s)	960, 1101, 2289, 2317, 2534, 2672, 2806
BsoBI	CYCGRG	1 cut(s)	396
Bsp119I	TTCGAA	1 cut(s)	352
Bsp1286I	GDGCHC	3 cut(s)	1160, 1217, 2783
Bsp13I	TCCGGA	2 cut(s)	884, 1762
Bsp1407I	TGTACA	1 cut(s)	1826
Bsp143I	GATC	6 cut(s)	221, 1040, 1722, 1957, 2227, 2331
Bsp1720I	GCTNAGC	2 cut(s)	84, 984
Bsp68I	TCGCGA	1 cut(s)	2145
BspACI	CCGC	3 cut(s)	897, 1478, 1544
BspANI	GGCC	7 cut(s)	960, 1101, 2289, 2317, 2534, 2672, 2806
BspCNI	CTCAG	6 cut(s)	97, 532, 825, 976, 1096, 1441
BspEI	TCCGGA	2 cut(s)	884, 1762
BspFNI	CGCG	2 cut(s)	1286, 2145
BspLI	GGNNCC	5 cut(s)	223, 515, 1783, 2518, 2535
BspMAI	CTGCAG	1 cut(s)	1407
BspMI	ACCTGC	1 cut(s)	1026
BspPI	GGATC	4 cut(s)	216, 229, 1717, 2235
BspQI	GCTCTTC	1 cut(s)	1165
BspT104I	TTCGAA	1 cut(s)	352
BspTI	CTTAAG	1 cut(s)	1177
BsrDI	GCAATG	1 cut(s)	1860
BsrFI	RCCGGY	1 cut(s)	1865
BsrGI	TGTACA	1 cut(s)	1826
BsrI	ACTGG	7 cut(s)	601, 741, 1015, 1739, 2244, 2318, 2672
BssAI	RCCGGY	1 cut(s)	1865
BssECI	CCNNGG	6 cut(s)	192, 225, 311, 395, 396, 2817
BssMI	GATC	6 cut(s)	221, 1040, 1722, 1957, 2227, 2331
Bst2UI	CCWGG	3 cut(s)	227, 312, 2309
Bst4CI	ACNGT	8 cut(s)	193, 268, 1232, 1824, 1843, 2585, 2630, 2851
Bst6I	CTCTTC	4 cut(s)	777, 1165, 1306, 2478
BstAFI	CTTAAG	1 cut(s)	1177
BstAPI	GCANNNNNTGC	1 cut(s)	270
BstAUI	TGTACA	1 cut(s)	1826
BstBI	TTCGAA	1 cut(s)	352
BstC8I	GCNNGC	6 cut(s)	12, 178, 958, 1468, 1657, 2831
BstDSI	CCRYGG	1 cut(s)	192
BstEII	GGTNACC	1 cut(s)	17
BstF5I	GGATG	5 cut(s)	940, 968, 1396, 2216, 2434
BstFNI	CGCG	2 cut(s)	1286, 2145
BstHHI	GCGC	1 cut(s)	2548
BstKTI	GATC	6 cut(s)	224, 1043, 1725, 1960, 2230, 2334
BstMAI	GTCTC	6 cut(s)	605, 1374, 1804, 1942, 2394, 2659
BstMBI	GATC	6 cut(s)	221, 1040, 1722, 1957, 2227, 2331
BstMWI	GCNNNNNNNGC	2 cut(s)	270, 1661
BstNI	CCWGG	3 cut(s)	227, 312, 2309
BstNSI	RCATGY	1 cut(s)	2833
BstPI	GGTNACC	1 cut(s)	17
BstSCI	CCNGG	8 cut(s)	225, 310, 395, 396, 2289, 2307, 2339, 2853
BstSFI	CTRYAG	1 cut(s)	1403
BstSLI	GKGCMC	2 cut(s)	1217, 2783
BstUI	CGCG	2 cut(s)	1286, 2145
BstV1I	GCAGC	6 cut(s)	75, 223, 1389, 1999, 2125, 2409
BstX2I	RGATCY	4 cut(s)	221, 1722, 1957, 2331
BstXI	CCANNNNNNTGG	1 cut(s)	524
BstYI	RGATCY	4 cut(s)	221, 1722, 1957, 2331
BsuI	GTATCC	2 cut(s)	593, 1565
BsuRI	GGCC	7 cut(s)	960, 1101, 2289, 2317, 2534, 2672, 2806
BtgI	CCRYGG	1 cut(s)	192
BtrI	CACGTC	1 cut(s)	2497
BtsCI	GGATG	5 cut(s)	940, 968, 1396, 2216, 2434
BtsI	GCAGTG	2 cut(s)	1333, 2201
BtsIMutI	CAGTG	4 cut(s)	264, 1333, 2201, 2885
BtuMI	TCGCGA	1 cut(s)	2145
BveI	ACCTGC	1 cut(s)	1026
Cac8I	GCNNGC	6 cut(s)	12, 178, 958, 1468, 1657, 2831
CfoI	GCGC	1 cut(s)	2548
Cfr10I	RCCGGY	1 cut(s)	1865
Cfr13I	GGNCC	8 cut(s)	809, 909, 1100, 1781, 2063, 2288, 2533, 2670
Cfr9I	CCCGGG	1 cut(s)	396
CseI	GACGC	1 cut(s)	977
Csp6I	GTAC	4 cut(s)	387, 689, 1827, 2245
CspCI	CAANNNNNGTGG	2 cut(s)	649, 684
CviQI	GTAC	4 cut(s)	387, 689, 1827, 2245
DpnI	GATC	6 cut(s)	223, 1042, 1724, 1959, 2229, 2333
DpnII	GATC	6 cut(s)	221, 1040, 1722, 1957, 2227, 2331
DraI	TTTAAA	4 cut(s)	636, 1137, 1819, 2154
DrdI	GACNNNNNNGTC	2 cut(s)	1265, 1779
DseDI	GACNNNNNNGTC	2 cut(s)	1265, 1779
Eam1104I	CTCTTC	4 cut(s)	777, 1165, 1306, 2478
EarI	CTCTTC	4 cut(s)	777, 1165, 1306, 2478
Ecl136II	GAGCTC	1 cut(s)	1158
Eco147I	AGGCCT	1 cut(s)	2806
Eco24I	GRGCYC	1 cut(s)	1160
Eco32I	GATATC	2 cut(s)	1603, 2879
Eco47I	GGWCC	4 cut(s)	809, 909, 1781, 2063
Eco53kI	GAGCTC	1 cut(s)	1158
Eco57I	CTGAAG	1 cut(s)	1146
Eco88I	CYCGRG	1 cut(s)	396
Eco91I	GGTNACC	1 cut(s)	17
EcoICRI	GAGCTC	1 cut(s)	1158
EcoO109I	RGGNCCY	1 cut(s)	1100
EcoO65I	GGTNACC	1 cut(s)	17
EcoRI	GAATTC	1 cut(s)	1185
EcoRII	CCWGG	3 cut(s)	225, 310, 2307
EcoRV	GATATC	2 cut(s)	1603, 2879
EcoT22I	ATGCAT	2 cut(s)	726, 1239
EcoT38I	GRGCYC	1 cut(s)	1160
Esp3I	CGTCTC	1 cut(s)	605
FalI	AAGNNNNNCTT	6 cut(s)	1947, 1979, 2066, 2098, 2632, 2664
FaqI	GGGAC	5 cut(s)	90, 489, 1762, 1767, 2837
FauI	CCCGC	1 cut(s)	904
FblI	GTMKAC	1 cut(s)	2070
Fnu4HI	GCNGC	6 cut(s)	89, 212, 1403, 2013, 2114, 2423
FokI	GGATG	5 cut(s)	947, 955, 1403, 2223, 2441
FriOI	GRGCYC	1 cut(s)	1160
Fsp4HI	GCNGC	6 cut(s)	89, 212, 1403, 2013, 2114, 2423
FspBI	CTAG	4 cut(s)	851, 923, 1578, 2195
GlaI	GCGC	1 cut(s)	2547
GluI	GCNGC	6 cut(s)	89, 212, 1403, 2013, 2114, 2423
HaeIII	GGCC	7 cut(s)	960, 1101, 2289, 2317, 2534, 2672, 2806
HgaI	GACGC	1 cut(s)	977
HhaI	GCGC	1 cut(s)	2548
Hin6I	GCGC	1 cut(s)	2546
HinP1I	GCGC	1 cut(s)	2546
HinfI	GANTC	7 cut(s)	748, 919, 1759, 1881, 2030, 2375, 2382
HphI	GGTGA	1 cut(s)	2836
Hpy166II	GTNNAC	9 cut(s)	308, 734, 1827, 1992, 2071, 2245, 2395, 2581, 2781
Hpy188I	TCNGA	8 cut(s)	492, 647, 1155, 1165, 1450, 2089, 2455, 2818
Hpy8I	GTNNAC	9 cut(s)	308, 734, 1827, 1992, 2071, 2245, 2395, 2581, 2781
HpyAV	CCTTC	9 cut(s)	211, 359, 734, 1295, 1313, 1624, 2045, 2695, 2831
HpyCH4III	ACNGT	8 cut(s)	193, 268, 1232, 1824, 1843, 2585, 2630, 2851
HpyCH4IV	ACGT	6 cut(s)	614, 1032, 1290, 1713, 1988, 2496
HpyF10VI	GCNNNNNNNGC	2 cut(s)	270, 1661
HpySE526I	ACGT	6 cut(s)	614, 1032, 1290, 1713, 1988, 2496
HspAI	GCGC	1 cut(s)	2546
Kpn2I	TCCGGA	2 cut(s)	884, 1762
Kzo9I	GATC	6 cut(s)	221, 1040, 1722, 1957, 2227, 2331
LguI	GCTCTTC	1 cut(s)	1165
LmnI	GCTCC	3 cut(s)	980, 1155, 2704
Lsp1109I	GCAGC	6 cut(s)	75, 223, 1389, 1999, 2125, 2409
LweI	GCATC	4 cut(s)	106, 163, 564, 2201
MaeI	CTAG	4 cut(s)	851, 923, 1578, 2195
MaeII	ACGT	6 cut(s)	614, 1032, 1290, 1713, 1988, 2496
MalI	GATC	6 cut(s)	223, 1042, 1724, 1959, 2229, 2333
MboI	GATC	6 cut(s)	221, 1040, 1722, 1957, 2227, 2331
MflI	RGATCY	4 cut(s)	221, 1722, 1957, 2331
MhlI	GDGCHC	3 cut(s)	1160, 1217, 2783
MlyI	GAGTC	1 cut(s)	2376
MmeI	TCCRAC	3 cut(s)	912, 1836, 2583
Mph1103I	ATGCAT	2 cut(s)	726, 1239
MroI	TCCGGA	2 cut(s)	884, 1762
MroXI	GAANNNNTTC	1 cut(s)	1634
MslI	CAYNNNNRTG	2 cut(s)	729, 2417
MspA1I	CMGCKG	4 cut(s)	10, 88, 592, 1796
MspCI	CTTAAG	1 cut(s)	1177
MspR9I	CCNGG	8 cut(s)	227, 312, 397, 398, 2291, 2309, 2341, 2855
Mva1269I	GAATGC	2 cut(s)	724, 2281
MvaI	CCWGG	3 cut(s)	227, 312, 2309
MvnI	CGCG	2 cut(s)	1286, 2145
MwoI	GCNNNNNNNGC	2 cut(s)	270, 1661
NciI	CCSGG	5 cut(s)	397, 398, 2291, 2341, 2855
NdeII	GATC	6 cut(s)	221, 1040, 1722, 1957, 2227, 2331
NlaIV	GGNNCC	5 cut(s)	223, 515, 1783, 2518, 2535
NmuCI	GTSAC	7 cut(s)	797, 1256, 1702, 2078, 2197, 2555, 2796
NruI	TCGCGA	1 cut(s)	2145
NsiI	ATGCAT	2 cut(s)	726, 1239
NspI	RCATGY	1 cut(s)	2833
NspV	TTCGAA	1 cut(s)	352
OliI	CACNNNNGTG	1 cut(s)	2417
PaeI	GCATGC	1 cut(s)	2833
PceI	AGGCCT	1 cut(s)	2806
PciSI	GCTCTTC	1 cut(s)	1165
PctI	GAATGC	2 cut(s)	724, 2281
PdmI	GAANNNNTTC	1 cut(s)	1634
PfeI	GAWTC	6 cut(s)	748, 919, 1759, 1881, 2030, 2375
PfoI	TCCNGGA	1 cut(s)	2853
PkrI	GCNGC	6 cut(s)	90, 213, 1404, 2014, 2115, 2424
PleI	GAGTC	1 cut(s)	2376
PpsI	GAGTC	1 cut(s)	2376
PshBI	ATTAAT	1 cut(s)	1242
Psp124BI	GAGCTC	1 cut(s)	1160
Psp6I	CCWGG	3 cut(s)	225, 310, 2307
PspEI	GGTNACC	1 cut(s)	17
PspGI	CCWGG	3 cut(s)	225, 310, 2307
PspN4I	GGNNCC	5 cut(s)	223, 515, 1783, 2518, 2535
PspPI	GGNCC	8 cut(s)	809, 909, 1100, 1781, 2063, 2288, 2533, 2670
PstI	CTGCAG	1 cut(s)	1407
PsuI	RGATCY	4 cut(s)	221, 1722, 1957, 2331
PvuII	CAGCTG	4 cut(s)	10, 88, 592, 1796
RruI	TCGCGA	1 cut(s)	2145
RsaI	GTAC	4 cut(s)	388, 690, 1828, 2246
RsaNI	GTAC	4 cut(s)	387, 689, 1827, 2245
RseI	CAYNNNNRTG	2 cut(s)	729, 2417
SacI	GAGCTC	1 cut(s)	1160
SapI	GCTCTTC	1 cut(s)	1165
SatI	GCNGC	6 cut(s)	89, 212, 1403, 2013, 2114, 2423
Sau3AI	GATC	6 cut(s)	221, 1040, 1722, 1957, 2227, 2331
Sau96I	GGNCC	8 cut(s)	809, 909, 1100, 1781, 2063, 2288, 2533, 2670
SchI	GAGTC	1 cut(s)	2376
ScrFI	CCNGG	8 cut(s)	227, 312, 397, 398, 2291, 2309, 2341, 2855
SduI	GDGCHC	3 cut(s)	1160, 1217, 2783
SfaNI	GCATC	4 cut(s)	106, 163, 564, 2201
SfcI	CTRYAG	1 cut(s)	1403
SfuI	TTCGAA	1 cut(s)	352
SinI	GGWCC	4 cut(s)	809, 909, 1781, 2063
SmaI	CCCGGG	1 cut(s)	398
SmiI	ATTTAAAT	1 cut(s)	1137
SmiMI	CAYNNNNRTG	2 cut(s)	729, 2417
SmlI	CTYRAG	4 cut(s)	293, 506, 1177, 2384
SmoI	CTYRAG	4 cut(s)	293, 506, 1177, 2384
SpeI	ACTAGT	1 cut(s)	1577
SphI	GCATGC	1 cut(s)	2833
SseBI	AGGCCT	1 cut(s)	2806
SsiI	CCGC	3 cut(s)	897, 1478, 1544
SspI	AATATT	3 cut(s)	760, 880, 1642
SspMI	CTAG	4 cut(s)	851, 923, 1578, 2195
SstI	GAGCTC	1 cut(s)	1160
StuI	AGGCCT	1 cut(s)	2806
StyD4I	CCNGG	8 cut(s)	225, 310, 395, 396, 2289, 2307, 2339, 2853
SwaI	ATTTAAAT	1 cut(s)	1137
TaaI	ACNGT	8 cut(s)	193, 268, 1232, 1824, 1843, 2585, 2630, 2851
TaiI	ACGT	6 cut(s)	617, 1035, 1293, 1716, 1991, 2499
TaqI	TCGA	4 cut(s)	352, 728, 1398, 1599
TatI	WGTACW	1 cut(s)	1826
TfiI	GAWTC	6 cut(s)	748, 919, 1759, 1881, 2030, 2375
TscAI	CASTG	4 cut(s)	271, 1333, 2208, 2892
TseFI	GTSAC	7 cut(s)	797, 1256, 1702, 2078, 2197, 2555, 2796
TseI	GCWGC	6 cut(s)	88, 211, 1402, 2012, 2113, 2422
Tsp45I	GTSAC	7 cut(s)	797, 1256, 1702, 2078, 2197, 2555, 2796
TspDTI	ATGAA	7 cut(s)	31, 929, 1375, 1888, 2085, 2385, 2496
TspGWI	ACGGA	1 cut(s)	2784
TspMI	CCCGGG	1 cut(s)	396
TspRI	CASTG	4 cut(s)	271, 1333, 2208, 2892
Vha464I	CTTAAG	1 cut(s)	1177
VneI	GTGCAC	1 cut(s)	2779
VpaK11BI	GGWCC	4 cut(s)	809, 909, 1781, 2063
VspI	ATTAAT	1 cut(s)	1242
XapI	RAATTY	9 cut(s)	550, 821, 838, 995, 1132, 1185, 1204, 1553, 2147
XbaI	TCTAGA	1 cut(s)	922
XceI	RCATGY	1 cut(s)	2833
XcmI	CCANNNNNNNNNTGG	3 cut(s)	2422, 2527, 2818
XmaI	CCCGGG	1 cut(s)	396
XmiI	GTMKAC	1 cut(s)	2070
XmnI	GAANNNNTTC	1 cut(s)	1634
XspI	CTAG	4 cut(s)	851, 923, 1578, 2195
Zsp2I	ATGCAT	2 cut(s)	726, 1239

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.