LRR receptor-like serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1247 |
| Acc36I | ACCTGC | 3 cut(s) | 489, 1102, 2910 |
| AccB1I | GGYRCC | 2 cut(s) | 88, 2515 |
| AccI | GTMKAC | 3 cut(s) | 289, 1553, 2052 |
| AciI | CCGC | 4 cut(s) | 1523, 2555, 2908, 2914 |
| AclWI | GGATC | 1 cut(s) | 1696 |
| AcoI | YGGCCR | 2 cut(s) | 2269, 2297 |
| AcsI | RAATTY | 3 cut(s) | 613, 945, 2950 |
| AcuI | CTGAAG | 1 cut(s) | 43 |
| AfaI | GTAC | 7 cut(s) | 273, 370, 1017, 1061, 1910, 2234, 2364 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 527, 1748, 2112, 2914 |
| AflII | CTTAAG | 2 cut(s) | 1159, 2309 |
| AflIII | ACRYGT | 2 cut(s) | 1391, 2967 |
| AhlI | ACTAGT | 1 cut(s) | 1556 |
| AjiI | CACGTC | 1 cut(s) | 2845 |
| AjnI | CCWGG | 2 cut(s) | 93, 903 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1895, 1927, 2025, 2057 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1517, 1549 |
| Alw21I | GWGCWC | 4 cut(s) | 1142, 1671, 2768, 2864 |
| Alw26I | GTCTC | 7 cut(s) | 194, 1251, 1260, 1278, 1353, 1938, 2892 |
| Alw44I | GTGCAC | 1 cut(s) | 2764 |
| AlwI | GGATC | 1 cut(s) | 1696 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 252 |
| Ama87I | CYCGRG | 3 cut(s) | 1085, 2487, 2929 |
| AoxI | GGCC | 6 cut(s) | 829, 1081, 2269, 2297, 2580, 2789 |
| ApaLI | GTGCAC | 1 cut(s) | 2764 |
| ApeKI | GCWGC | 7 cut(s) | 15, 18, 51, 54, 1994, 2095, 2404 |
| ApoI | RAATTY | 3 cut(s) | 613, 945, 2950 |
| AseI | ATTAAT | 1 cut(s) | 536 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1613 |
| AspLEI | GCGC | 1 cut(s) | 1572 |
| AspS9I | GGNCC | 5 cut(s) | 725, 1082, 1760, 2045, 2499 |
| AsuC2I | CCSGG | 2 cut(s) | 294, 2381 |
| AsuHPI | GGTGA | 4 cut(s) | 2369, 2531, 2821, 2930 |
| AsuII | TTCGAA | 2 cut(s) | 1418, 2973 |
| AvaI | CYCGRG | 3 cut(s) | 1085, 2487, 2929 |
| AvaII | GGWCC | 4 cut(s) | 725, 1760, 2045, 2499 |
| BaeGI | GKGCMC | 2 cut(s) | 93, 2768 |
| BalI | TGGCCA | 1 cut(s) | 2299 |
| BanI | GGYRCC | 2 cut(s) | 88, 2515 |
| BanII | GRGCYC | 1 cut(s) | 1142 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 1052, 1084, 2346, 2378 |
| BauI | CACGAG | 1 cut(s) | 2256 |
| BbsI | GAAGAC | 4 cut(s) | 1021, 1163, 1505, 2477 |
| Bbv12I | GWGCWC | 4 cut(s) | 1142, 1671, 2768, 2864 |
| BbvI | GCAGC | 7 cut(s) | 27, 30, 38, 66, 1981, 2107, 2391 |
| BccI | CCATC | 8 cut(s) | 127, 737, 1031, 1420, 1633, 1949, 2066, 2137 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1076, 1110, 2105, 2139 |
| BciT130I | CCWGG | 2 cut(s) | 95, 905 |
| BclI | TGATCA | 1 cut(s) | 2920 |
| BcnI | CCSGG | 2 cut(s) | 294, 2381 |
| BcoDI | GTCTC | 7 cut(s) | 194, 1251, 1260, 1278, 1353, 1938, 2892 |
| BcuI | ACTAGT | 1 cut(s) | 1556 |
| BfaI | CTAG | 5 cut(s) | 833, 1557, 1871, 2339, 2793 |
| BfmI | CTRYAG | 1 cut(s) | 52 |
| BfrI | CTTAAG | 2 cut(s) | 1159, 2309 |
| BfuAI | ACCTGC | 3 cut(s) | 489, 1102, 2910 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2305 |
| BisI | GCNGC | 7 cut(s) | 16, 19, 52, 55, 1995, 2096, 2405 |
| BlsI | GCNGC | 7 cut(s) | 17, 20, 53, 56, 1996, 2097, 2406 |
| BmcAI | AGTACT | 1 cut(s) | 273 |
| Bme1390I | CCNGG | 4 cut(s) | 95, 294, 905, 2381 |
| Bme18I | GGWCC | 4 cut(s) | 725, 1760, 2045, 2499 |
| BmeT110I | CYCGRG | 3 cut(s) | 1085, 2487, 2929 |
| BmgBI | CACGTC | 1 cut(s) | 2845 |
| BmgT120I | GGNCC | 5 cut(s) | 725, 1082, 1760, 2045, 2499 |
| BmiI | GGNNCC | 8 cut(s) | 90, 727, 1084, 1092, 1109, 1762, 2500, 2517 |
| BmrFI | CCNGG | 4 cut(s) | 95, 294, 905, 2381 |
| BmsI | GCATC | 3 cut(s) | 97, 493, 2789 |
| BpiI | GAAGAC | 4 cut(s) | 1021, 1163, 1505, 2477 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1851, 1883 |
| BpmI | CTGGAG | 4 cut(s) | 528, 674, 888, 1377 |
| Bpu10I | CCTNAGC | 1 cut(s) | 522 |
| Bpu14I | TTCGAA | 2 cut(s) | 1418, 2973 |
| BpuEI | CTTGAG | 2 cut(s) | 260, 1792 |
| BpuMI | CCSGG | 2 cut(s) | 294, 2381 |
| Bsa29I | ATCGAT | 1 cut(s) | 2697 |
| BsaI | GGTCTC | 1 cut(s) | 2892 |
| BsaJI | CCNNGG | 5 cut(s) | 93, 1086, 1782, 2583, 2802 |
| BsaWI | WCCGGW | 1 cut(s) | 2518 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 1257, 1287, 1517, 1547, 1839, 1869 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 527, 1748, 2112, 2914 |
| Bse1I | ACTGG | 5 cut(s) | 583, 997, 1360, 2300, 2995 |
| BseBI | CCWGG | 2 cut(s) | 95, 905 |
| BseCI | ATCGAT | 1 cut(s) | 2697 |
| BseDI | CCNNGG | 5 cut(s) | 93, 1086, 1782, 2583, 2802 |
| BseGI | GGATG | 7 cut(s) | 103, 729, 1042, 1375, 2167, 2198, 2563 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 527, 1748, 2112, 2914 |
| BseMII | CTCAG | 5 cut(s) | 69, 207, 513, 2706, 3018 |
| BseNI | ACTGG | 5 cut(s) | 583, 997, 1360, 2300, 2995 |
| BseRI | GAGGAG | 2 cut(s) | 1764, 2480 |
| BseSI | GKGCMC | 2 cut(s) | 93, 2768 |
| BseXI | GCAGC | 7 cut(s) | 27, 30, 38, 66, 1981, 2107, 2391 |
| BseYI | CCCAGC | 1 cut(s) | 2495 |
| BsgI | GTGCAG | 2 cut(s) | 34, 2888 |
| BshFI | GGCC | 6 cut(s) | 831, 1083, 2271, 2299, 2582, 2791 |
| BshNI | GGYRCC | 2 cut(s) | 88, 2515 |
| BshVI | ATCGAT | 1 cut(s) | 2697 |
| BsiHKAI | GWGCWC | 4 cut(s) | 1142, 1671, 2768, 2864 |
| BsiHKCI | CYCGRG | 3 cut(s) | 1085, 2487, 2929 |
| BsiSI | CCGG | 3 cut(s) | 294, 2380, 2519 |
| BslFI | GGGAC | 5 cut(s) | 805, 1746, 2396, 2512, 2822 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 527, 1748, 2112, 2914 |
| BsmAI | GTCTC | 7 cut(s) | 194, 1251, 1260, 1278, 1353, 1938, 2892 |
| BsmBI | CGTCTC | 2 cut(s) | 1278, 1938 |
| BsmFI | GGGAC | 5 cut(s) | 805, 1746, 2396, 2512, 2822 |
| BsmI | GAATGC | 1 cut(s) | 242 |
| BsnI | GGCC | 6 cut(s) | 831, 1083, 2271, 2299, 2582, 2791 |
| Bso31I | GGTCTC | 1 cut(s) | 2892 |
| BsoBI | CYCGRG | 3 cut(s) | 1085, 2487, 2929 |
| Bsp119I | TTCGAA | 2 cut(s) | 1418, 2973 |
| Bsp1286I | GDGCHC | 5 cut(s) | 93, 1142, 1671, 2768, 2864 |
| Bsp143I | GATC | 7 cut(s) | 408, 1051, 1701, 2313, 2694, 2739, 2920 |
| BspACI | CCGC | 4 cut(s) | 1523, 2555, 2908, 2914 |
| BspANI | GGCC | 6 cut(s) | 831, 1083, 2271, 2299, 2582, 2791 |
| BspCNI | CTCAG | 5 cut(s) | 70, 208, 514, 2707, 3017 |
| BspDI | ATCGAT | 1 cut(s) | 2697 |
| BspLI | GGNNCC | 8 cut(s) | 90, 727, 1084, 1092, 1109, 1762, 2500, 2517 |
| BspMAI | CTGCAG | 1 cut(s) | 56 |
| BspMI | ACCTGC | 3 cut(s) | 489, 1102, 2910 |
| BspPI | GGATC | 1 cut(s) | 1696 |
| BspQI | GCTCTTC | 1 cut(s) | 1147 |
| BspT104I | TTCGAA | 2 cut(s) | 1418, 2973 |
| BspT107I | GGYRCC | 2 cut(s) | 88, 2515 |
| BspTI | CTTAAG | 2 cut(s) | 1159, 2309 |
| BspTNI | GGTCTC | 1 cut(s) | 2892 |
| BsrI | ACTGG | 5 cut(s) | 583, 997, 1360, 2300, 2995 |
| BssECI | CCNNGG | 5 cut(s) | 93, 1086, 1782, 2583, 2802 |
| BssMI | GATC | 7 cut(s) | 408, 1051, 1701, 2313, 2694, 2739, 2920 |
| BssNAI | GTATAC | 1 cut(s) | 1554 |
| BssSI | CACGAG | 1 cut(s) | 2256 |
| BssT1I | CCWWGG | 1 cut(s) | 2583 |
| Bst1107I | GTATAC | 1 cut(s) | 1554 |
| Bst2BI | CACGAG | 1 cut(s) | 2256 |
| Bst2UI | CCWGG | 2 cut(s) | 95, 905 |
| Bst4CI | ACNGT | 7 cut(s) | 248, 857, 1249, 1507, 1533, 2567, 2962 |
| Bst6I | CTCTTC | 4 cut(s) | 192, 420, 1147, 2457 |
| BstAFI | CTTAAG | 2 cut(s) | 1159, 2309 |
| BstBI | TTCGAA | 2 cut(s) | 1418, 2973 |
| BstC8I | GCNNGC | 8 cut(s) | 65, 299, 708, 1447, 1568, 1918, 2816, 2912 |
| BstDSI | CCRYGG | 1 cut(s) | 1782 |
| BstEII | GGTNACC | 1 cut(s) | 2537 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1746 |
| BstF5I | GGATG | 7 cut(s) | 103, 729, 1042, 1375, 2167, 2198, 2563 |
| BstHHI | GCGC | 1 cut(s) | 1572 |
| BstKTI | GATC | 7 cut(s) | 411, 1054, 1704, 2316, 2697, 2742, 2923 |
| BstMAI | GTCTC | 7 cut(s) | 194, 1251, 1260, 1278, 1353, 1938, 2892 |
| BstMBI | GATC | 7 cut(s) | 408, 1051, 1701, 2313, 2694, 2739, 2920 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 60, 168, 1640, 2305, 2911 |
| BstNI | CCWGG | 2 cut(s) | 95, 905 |
| BstNSI | RCATGY | 5 cut(s) | 301, 710, 1223, 2800, 2818 |
| BstPI | GGTNACC | 1 cut(s) | 2537 |
| BstSCI | CCNGG | 4 cut(s) | 93, 292, 903, 2379 |
| BstSFI | CTRYAG | 1 cut(s) | 52 |
| BstSLI | GKGCMC | 2 cut(s) | 93, 2768 |
| BstV1I | GCAGC | 7 cut(s) | 27, 30, 38, 66, 1981, 2107, 2391 |
| BstV2I | GAAGAC | 4 cut(s) | 1021, 1163, 1505, 2477 |
| BstX2I | RGATCY | 1 cut(s) | 1701 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 384, 888 |
| BstYI | RGATCY | 1 cut(s) | 1701 |
| BstZ17I | GTATAC | 1 cut(s) | 1554 |
| Bsu15I | ATCGAT | 1 cut(s) | 2697 |
| BsuRI | GGCC | 6 cut(s) | 831, 1083, 2271, 2299, 2582, 2791 |
| BsuTUI | ATCGAT | 1 cut(s) | 2697 |
| BtgI | CCRYGG | 1 cut(s) | 1782 |
| BtrI | CACGTC | 1 cut(s) | 2845 |
| BtsCI | GGATG | 7 cut(s) | 103, 729, 1042, 1375, 2167, 2198, 2563 |
| BtsI | GCAGTG | 1 cut(s) | 1312 |
| BtsIMutI | CAGTG | 5 cut(s) | 253, 1312, 1353, 1684, 2602 |
| BveI | ACCTGC | 3 cut(s) | 489, 1102, 2910 |
| Cac8I | GCNNGC | 8 cut(s) | 65, 299, 708, 1447, 1568, 1918, 2816, 2912 |
| CaiI | CAGNNNCTG | 1 cut(s) | 252 |
| CfoI | GCGC | 1 cut(s) | 1572 |
| Cfr13I | GGNCC | 5 cut(s) | 725, 1082, 1760, 2045, 2499 |
| ClaI | ATCGAT | 1 cut(s) | 2697 |
| CseI | GACGC | 1 cut(s) | 2482 |
| Csp6I | GTAC | 7 cut(s) | 272, 369, 1016, 1060, 1909, 2233, 2363 |
| CviQI | GTAC | 7 cut(s) | 272, 369, 1016, 1060, 1909, 2233, 2363 |
| DpnI | GATC | 7 cut(s) | 410, 1053, 1703, 2315, 2696, 2741, 2922 |
| DpnII | GATC | 7 cut(s) | 408, 1051, 1701, 2313, 2694, 2739, 2920 |
| DraI | TTTAAA | 1 cut(s) | 1980 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1247 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1247 |
| EaeI | YGGCCR | 2 cut(s) | 2269, 2297 |
| Eam1104I | CTCTTC | 4 cut(s) | 192, 420, 1147, 2457 |
| EarI | CTCTTC | 4 cut(s) | 192, 420, 1147, 2457 |
| Ecl136II | GAGCTC | 1 cut(s) | 1140 |
| Eco130I | CCWWGG | 1 cut(s) | 2583 |
| Eco147I | AGGCCT | 2 cut(s) | 831, 2791 |
| Eco24I | GRGCYC | 1 cut(s) | 1142 |
| Eco31I | GGTCTC | 1 cut(s) | 2892 |
| Eco32I | GATATC | 1 cut(s) | 1582 |
| Eco47I | GGWCC | 4 cut(s) | 725, 1760, 2045, 2499 |
| Eco53kI | GAGCTC | 1 cut(s) | 1140 |
| Eco57I | CTGAAG | 1 cut(s) | 43 |
| Eco88I | CYCGRG | 3 cut(s) | 1085, 2487, 2929 |
| Eco91I | GGTNACC | 1 cut(s) | 2537 |
| EcoICRI | GAGCTC | 1 cut(s) | 1140 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1746 |
| EcoO109I | RGGNCCY | 2 cut(s) | 725, 1082 |
| EcoO65I | GGTNACC | 1 cut(s) | 2537 |
| EcoRII | CCWGG | 2 cut(s) | 93, 903 |
| EcoRV | GATATC | 1 cut(s) | 1582 |
| EcoT14I | CCWWGG | 1 cut(s) | 2583 |
| EcoT22I | ATGCAT | 1 cut(s) | 708 |
| EcoT38I | GRGCYC | 1 cut(s) | 1142 |
| ErhI | CCWWGG | 1 cut(s) | 2583 |
| Esp3I | CGTCTC | 2 cut(s) | 1278, 1938 |
| FalI | AAGNNNNNCTT | 3 cut(s) | 1304, 1336, 3016 |
| FaqI | GGGAC | 5 cut(s) | 805, 1746, 2396, 2512, 2822 |
| FauI | CCCGC | 1 cut(s) | 2907 |
| FbaI | TGATCA | 1 cut(s) | 2920 |
| FblI | GTMKAC | 3 cut(s) | 289, 1553, 2052 |
| Fnu4HI | GCNGC | 7 cut(s) | 16, 19, 52, 55, 1995, 2096, 2405 |
| FokI | GGATG | 7 cut(s) | 110, 716, 1049, 1382, 2154, 2205, 2570 |
| FriOI | GRGCYC | 1 cut(s) | 1142 |
| Fsp4HI | GCNGC | 7 cut(s) | 16, 19, 52, 55, 1995, 2096, 2405 |
| FspBI | CTAG | 5 cut(s) | 833, 1557, 1871, 2339, 2793 |
| GlaI | GCGC | 1 cut(s) | 1571 |
| GluI | GCNGC | 7 cut(s) | 16, 19, 52, 55, 1995, 2096, 2405 |
| GsaI | CCCAGC | 1 cut(s) | 2499 |
| GsuI | CTGGAG | 4 cut(s) | 528, 674, 888, 1377 |
| HaeIII | GGCC | 6 cut(s) | 831, 1083, 2271, 2299, 2582, 2791 |
| HapII | CCGG | 3 cut(s) | 294, 2380, 2519 |
| HgaI | GACGC | 1 cut(s) | 2482 |
| HhaI | GCGC | 1 cut(s) | 1572 |
| Hin6I | GCGC | 1 cut(s) | 1570 |
| HinP1I | GCGC | 1 cut(s) | 1570 |
| HincII | GTYRAC | 2 cut(s) | 402, 2722 |
| HindII | GTYRAC | 2 cut(s) | 402, 2722 |
| HindIII | AAGCTT | 1 cut(s) | 2453 |
| HpaII | CCGG | 3 cut(s) | 294, 2380, 2519 |
| HphI | GGTGA | 4 cut(s) | 2369, 2531, 2821, 2930 |
| HpyAV | CCTTC | 9 cut(s) | 442, 744, 823, 1274, 1292, 1603, 2027, 2198, 2816 |
| HpyCH4III | ACNGT | 7 cut(s) | 248, 857, 1249, 1507, 1533, 2567, 2962 |
| HpyCH4IV | ACGT | 5 cut(s) | 885, 1393, 1550, 2844, 2969 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 60, 168, 1640, 2305, 2911 |
| HpySE526I | ACGT | 5 cut(s) | 885, 1393, 1550, 2844, 2969 |
| HspAI | GCGC | 1 cut(s) | 1570 |
| Ksp22I | TGATCA | 1 cut(s) | 2920 |
| Kzo9I | GATC | 7 cut(s) | 408, 1051, 1701, 2313, 2694, 2739, 2920 |
| LguI | GCTCTTC | 1 cut(s) | 1147 |
| LmnI | GCTCC | 4 cut(s) | 212, 1137, 1859, 2689 |
| Lsp1109I | GCAGC | 7 cut(s) | 27, 30, 38, 66, 1981, 2107, 2391 |
| LweI | GCATC | 3 cut(s) | 97, 493, 2789 |
| MaeI | CTAG | 5 cut(s) | 833, 1557, 1871, 2339, 2793 |
| MaeII | ACGT | 5 cut(s) | 885, 1393, 1550, 2844, 2969 |
| MaeIII | GTNAC | 9 cut(s) | 190, 391, 755, 857, 1394, 1496, 1681, 1840, 2537 |
| MalI | GATC | 7 cut(s) | 410, 1053, 1703, 2315, 2696, 2741, 2922 |
| MboI | GATC | 7 cut(s) | 408, 1051, 1701, 2313, 2694, 2739, 2920 |
| MfeI | CAATTG | 1 cut(s) | 1192 |
| MflI | RGATCY | 1 cut(s) | 1701 |
| MhlI | GDGCHC | 5 cut(s) | 93, 1142, 1671, 2768, 2864 |
| MlsI | TGGCCA | 1 cut(s) | 2299 |
| MluNI | TGGCCA | 1 cut(s) | 2299 |
| MlyI | GAGTC | 3 cut(s) | 89, 173, 2988 |
| MmeI | TCCRAC | 1 cut(s) | 2667 |
| Mox20I | TGGCCA | 1 cut(s) | 2299 |
| Mph1103I | ATGCAT | 1 cut(s) | 708 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1613 |
| MscI | TGGCCA | 1 cut(s) | 2299 |
| MslI | CAYNNNNRTG | 1 cut(s) | 711 |
| Msp20I | TGGCCA | 1 cut(s) | 2299 |
| MspA1I | CMGCKG | 2 cut(s) | 51, 2914 |
| MspCI | CTTAAG | 2 cut(s) | 1159, 2309 |
| MspI | CCGG | 3 cut(s) | 294, 2380, 2519 |
| MspR9I | CCNGG | 4 cut(s) | 95, 294, 905, 2381 |
| MunI | CAATTG | 1 cut(s) | 1192 |
| Mva1269I | GAATGC | 1 cut(s) | 242 |
| MvaI | CCWGG | 2 cut(s) | 95, 905 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 60, 168, 1640, 2305, 2911 |
| NciI | CCSGG | 2 cut(s) | 294, 2381 |
| NdeII | GATC | 7 cut(s) | 408, 1051, 1701, 2313, 2694, 2739, 2920 |
| NlaIV | GGNNCC | 8 cut(s) | 90, 727, 1084, 1092, 1109, 1762, 2500, 2517 |
| NmeAIII | GCCGAG | 1 cut(s) | 2297 |
| NmuCI | GTSAC | 3 cut(s) | 1681, 1840, 2537 |
| NsiI | ATGCAT | 1 cut(s) | 708 |
| NspI | RCATGY | 5 cut(s) | 301, 710, 1223, 2800, 2818 |
| NspV | TTCGAA | 2 cut(s) | 1418, 2973 |
| PaeI | GCATGC | 3 cut(s) | 301, 710, 2818 |
| PaeR7I | CTCGAG | 1 cut(s) | 2929 |
| PceI | AGGCCT | 2 cut(s) | 831, 2791 |
| PciSI | GCTCTTC | 1 cut(s) | 1147 |
| PctI | GAATGC | 1 cut(s) | 242 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1613 |
| PkrI | GCNGC | 7 cut(s) | 17, 20, 53, 56, 1996, 2097, 2406 |
| PleI | GAGTC | 3 cut(s) | 88, 173, 2988 |
| PpsI | GAGTC | 3 cut(s) | 88, 173, 2988 |
| PpuMI | RGGWCCY | 1 cut(s) | 725 |
| PshBI | ATTAAT | 1 cut(s) | 536 |
| Psp124BI | GAGCTC | 1 cut(s) | 1142 |
| Psp5II | RGGWCCY | 1 cut(s) | 725 |
| Psp6I | CCWGG | 2 cut(s) | 93, 903 |
| PspEI | GGTNACC | 1 cut(s) | 2537 |
| PspFI | CCCAGC | 1 cut(s) | 2495 |
| PspGI | CCWGG | 2 cut(s) | 93, 903 |
| PspN4I | GGNNCC | 8 cut(s) | 90, 727, 1084, 1092, 1109, 1762, 2500, 2517 |
| PspPI | GGNCC | 5 cut(s) | 725, 1082, 1760, 2045, 2499 |
| PspPPI | RGGWCCY | 1 cut(s) | 725 |
| PspXI | VCTCGAGB | 1 cut(s) | 2929 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 828, 860 |
| PstI | CTGCAG | 1 cut(s) | 56 |
| PstNI | CAGNNNCTG | 1 cut(s) | 252 |
| PsuI | RGATCY | 1 cut(s) | 1701 |
| PvuII | CAGCTG | 1 cut(s) | 51 |
| RsaI | GTAC | 7 cut(s) | 273, 370, 1017, 1061, 1910, 2234, 2364 |
| RsaNI | GTAC | 7 cut(s) | 272, 369, 1016, 1060, 1909, 2233, 2363 |
| RseI | CAYNNNNRTG | 1 cut(s) | 711 |
| SacI | GAGCTC | 1 cut(s) | 1142 |
| SapI | GCTCTTC | 1 cut(s) | 1147 |
| SatI | GCNGC | 7 cut(s) | 16, 19, 52, 55, 1995, 2096, 2405 |
| Sau3AI | GATC | 7 cut(s) | 408, 1051, 1701, 2313, 2694, 2739, 2920 |
| Sau96I | GGNCC | 5 cut(s) | 725, 1082, 1760, 2045, 2499 |
| ScaI | AGTACT | 1 cut(s) | 273 |
| SchI | GAGTC | 3 cut(s) | 89, 173, 2988 |
| ScrFI | CCNGG | 4 cut(s) | 95, 294, 905, 2381 |
| SduI | GDGCHC | 5 cut(s) | 93, 1142, 1671, 2768, 2864 |
| SfaNI | GCATC | 3 cut(s) | 97, 493, 2789 |
| SfcI | CTRYAG | 1 cut(s) | 52 |
| Sfr274I | CTCGAG | 1 cut(s) | 2929 |
| SfuI | TTCGAA | 2 cut(s) | 1418, 2973 |
| SinI | GGWCC | 4 cut(s) | 725, 1760, 2045, 2499 |
| SlaI | CTCGAG | 1 cut(s) | 2929 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 711 |
| SmlI | CTYRAG | 5 cut(s) | 275, 1159, 1807, 2309, 2929 |
| SmoI | CTYRAG | 5 cut(s) | 275, 1159, 1807, 2309, 2929 |
| SpeI | ACTAGT | 1 cut(s) | 1556 |
| SphI | GCATGC | 3 cut(s) | 301, 710, 2818 |
| SseBI | AGGCCT | 2 cut(s) | 831, 2791 |
| SsiI | CCGC | 4 cut(s) | 1523, 2555, 2908, 2914 |
| SspI | AATATT | 3 cut(s) | 463, 1048, 1621 |
| SspMI | CTAG | 5 cut(s) | 833, 1557, 1871, 2339, 2793 |
| SstI | GAGCTC | 1 cut(s) | 1142 |
| StuI | AGGCCT | 2 cut(s) | 831, 2791 |
| StyD4I | CCNGG | 4 cut(s) | 93, 292, 903, 2379 |
| StyI | CCWWGG | 1 cut(s) | 2583 |
| TaaI | ACNGT | 7 cut(s) | 248, 857, 1249, 1507, 1533, 2567, 2962 |
| TaiI | ACGT | 5 cut(s) | 888, 1396, 1553, 2847, 2972 |
| TaqI | TCGA | 8 cut(s) | 407, 915, 1418, 2010, 2697, 2930, 2973, 2997 |
| TatI | WGTACW | 3 cut(s) | 271, 2232, 2362 |
| TscAI | CASTG | 5 cut(s) | 253, 1312, 1360, 1684, 2602 |
| TseFI | GTSAC | 3 cut(s) | 1681, 1840, 2537 |
| TseI | GCWGC | 7 cut(s) | 15, 18, 51, 54, 1994, 2095, 2404 |
| Tsp45I | GTSAC | 3 cut(s) | 1681, 1840, 2537 |
| TspDTI | ATGAA | 6 cut(s) | 114, 1293, 1354, 2177, 2367, 2475 |
| TspGWI | ACGGA | 3 cut(s) | 1302, 1771, 1962 |
| TspRI | CASTG | 5 cut(s) | 253, 1312, 1360, 1684, 2602 |
| Vha464I | CTTAAG | 2 cut(s) | 1159, 2309 |
| VneI | GTGCAC | 1 cut(s) | 2764 |
| VpaK11BI | GGWCC | 4 cut(s) | 725, 1760, 2045, 2499 |
| VspI | ATTAAT | 1 cut(s) | 536 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1746 |
| XapI | RAATTY | 3 cut(s) | 613, 945, 2950 |
| XceI | RCATGY | 5 cut(s) | 301, 710, 1223, 2800, 2818 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2509 |
| XhoI | CTCGAG | 1 cut(s) | 2929 |
| XmiI | GTMKAC | 3 cut(s) | 289, 1553, 2052 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1613 |
| XspI | CTAG | 5 cut(s) | 833, 1557, 1871, 2339, 2793 |
| ZrmI | AGTACT | 1 cut(s) | 273 |
| Zsp2I | ATGCAT | 1 cut(s) | 708 |