MD00G1031500.v1.1

C1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0014254
Physical Location & Seq
Forward (+)
5416541 .. 5417951
1411 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1031500.v1.1.491

Sequence Viewer

Length: 906 bp
ATGATTAGCAATAAACCCTTGAAGAAGACAAGAACTTTTCTCCTAAAGAGGTCAGAATCAACTACCCCCCAATCCCAATCTCAACCCCAATCCCAATCCCAATCCCAACCCACAAAGTTGGATAATATTGTCCCTAGAAATAATAAGTCTTCTTTGCTCGAATTCCCCACATCTCCTCAACTGGTATTTGGGGAAGAGATACTTCATTTCGGTCATCCACAACACCCTCTGTCCCAAGTGATCCTGCCCGACCTCTTCACCTGCGCCGGCTGCAAGGAGTACGGCGCTGGCAAGAGGTTCATTTGCCAACAATGTGACTTTCAGCTCCATGATTTTTGTGCCTTGGCTCCTCCAGCTCTAAAGAACCATCCCTTCCACTTCCAACACCAACTTGTTTTCTATTCTAAAGCAGTTAAAGGAGGAATTGCACAATCCAAGTGCGATGTTTGTCACAGGCCAAGCAAAGGTTATGCTTTCAGATGCAGCGCATGCAGCTTCCAGATGCATCCTTGCTGTGCGATGTTGTCTTCAGAAATAAACCTGCAGACTCACCCACATACCCTAAGGCTTTTACCAGCAGCAGCAAGTGGTAGCATTGGCGGAGATTCTTCTGGTAGCAGTTTTGTTTGTGCGGAGTGCAGGAGGAAGAAGTCGGGCAGGGTTTACCACTGCAAGGTGTGTGATTACCATATTCATGCAGTTTGCGCCAAGAACATGATAAATGGGCTGCAAGAAAACGGCCTCAAGGGGCGAGAAAAGCCGAGTGTGTTGGGGACTGCTGCTCGCCTCGCATCTCAAGTCGTCATTGAATTCATCGGCGGCCTCATTGAGGGGCTTGGGGAAGGTGTTGCCGAAGTTTTCGTTCAGAATATTGCCCGATCAGGAAGACCCAATGCCCCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2

Protein Analysis

302

Amino Acids

32.92

Weight (kDa)

9.13

Isoelectric Point (pI)

68.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C1_2 PF03107 71 - 114 5.6e-08 C1 domain
C1_2 PF03107 125 - 173 2.7e-06 C1 domain
C1_2 PF03107 183 - 236 5.5e-09 C1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20990
fragaria_vesca FvH4_5g26520
malus_domestica MD00G1031500.v1.1 MD06G1001400.v1.1
prunus_persica Prupe.5G002200_v2.0.a1
pyrus_communis pycom06g00150 pycom16g26300
rosa_chinensis RchiOBHm_Chr7g0217421
rosa_laevigata RLG00000002478
rosa_multiflora Rmu_sc0000145.1_g000014
rosa_roxburghii Rroxscaffold_3G00242090
rosa_rugosa Rorug07G0169000
rosa_samantha Rh7AG308700 Rh7BG299400 Rh7CG328100 Rh7DG308000
rosa_wichuraiana Rw7G026180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 269
Acc36I ACCTGC 2 cut(s) 269, 549
AciI CCGC 3 cut(s) 600, 632, 819
AclWI GGATC 1 cut(s) 235
AcsI RAATTY 2 cut(s) 161, 809
AcuI CTGAAG 1 cut(s) 513
AfaI GTAC 1 cut(s) 281
AfiI CCNNNNNNNGG 3 cut(s) 464, 673, 829
AgsI TTSAA 2 cut(s) 22, 809
AluBI AGCT 3 cut(s) 325, 356, 495
AluI AGCT 3 cut(s) 325, 356, 495
AlwI GGATC 1 cut(s) 235
AoxI GGCC 3 cut(s) 455, 739, 820
ApeKI GCWGC 7 cut(s) 270, 483, 492, 578, 581, 727, 779
ApoI RAATTY 2 cut(s) 161, 809
AspLEI GCGC 4 cut(s) 266, 287, 488, 707
AsuHPI GGTGA 2 cut(s) 250, 542
AxyI CCTNAGG 1 cut(s) 563
BbsI GAAGAC 4 cut(s) 32, 141, 519, 892
BbvI GCAGC 7 cut(s) 257, 495, 504, 590, 593, 714, 766
BccI CCATC 1 cut(s) 375
BceAI ACGGC 2 cut(s) 298, 754
BfaI CTAG 1 cut(s) 135
BfmI CTRYAG 1 cut(s) 542
BfoI RGCGCY 1 cut(s) 288
BfuAI ACCTGC 2 cut(s) 269, 549
BisI GCNGC 8 cut(s) 271, 484, 493, 579, 582, 728, 780, 820
BlsI GCNGC 8 cut(s) 272, 485, 494, 580, 583, 729, 781, 821
BmiI GGNNCC 1 cut(s) 348
BmsI GCATC 4 cut(s) 470, 492, 514, 800
BpiI GAAGAC 4 cut(s) 32, 141, 519, 892
BpmI CTGGAG 1 cut(s) 336
BpuEI CTTGAG 2 cut(s) 728, 780
BsaJI CCNNGG 1 cut(s) 342
Bsc4I CCNNNNNNNGG 3 cut(s) 464, 673, 829
Bse118I RCCGGY 1 cut(s) 266
Bse1I ACTGG 1 cut(s) 186
Bse21I CCTNAGG 1 cut(s) 563
BseDI CCNNGG 1 cut(s) 342
BseGI GGATG 3 cut(s) 214, 367, 505
BseLI CCNNNNNNNGG 3 cut(s) 464, 673, 829
BseNI ACTGG 1 cut(s) 186
BseRI GAGGAG 2 cut(s) 165, 339
BseXI GCAGC 7 cut(s) 257, 495, 504, 590, 593, 714, 766
BsgI GTGCAG 1 cut(s) 658
BshFI GGCC 3 cut(s) 457, 741, 822
BsiSI CCGG 1 cut(s) 267
BslFI GGGAC 3 cut(s) 116, 217, 787
BslI CCNNNNNNNGG 3 cut(s) 464, 673, 829
BsmFI GGGAC 3 cut(s) 116, 217, 787
BsnI GGCC 3 cut(s) 457, 741, 822
Bsp143I GATC 2 cut(s) 240, 878
BspACI CCGC 3 cut(s) 600, 632, 819
BspANI GGCC 3 cut(s) 457, 741, 822
BspLI GGNNCC 1 cut(s) 348
BspMAI CTGCAG 1 cut(s) 546
BspMI ACCTGC 2 cut(s) 269, 549
BspPI GGATC 1 cut(s) 235
BsrFI RCCGGY 1 cut(s) 266
BsrI ACTGG 1 cut(s) 186
BssAI RCCGGY 1 cut(s) 266
BssECI CCNNGG 1 cut(s) 342
BssMI GATC 2 cut(s) 240, 878
BssT1I CCWWGG 1 cut(s) 342
Bst6I CTCTTC 2 cut(s) 189, 260
BstAPI GCANNNNNTGC 1 cut(s) 489
BstC8I GCNNGC 4 cut(s) 268, 289, 490, 784
BstDEI CTNAG 1 cut(s) 563
BstENI CCTNNNNNAGG 1 cut(s) 827
BstF5I GGATG 3 cut(s) 214, 367, 505
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 4 cut(s) 266, 287, 488, 707
BstKTI GATC 2 cut(s) 243, 881
BstMBI GATC 2 cut(s) 240, 878
BstMWI GCNNNNNNNGC 7 cut(s) 270, 353, 489, 492, 704, 757, 788
BstNSI RCATGY 1 cut(s) 492
BstSFI CTRYAG 1 cut(s) 542
BstV1I GCAGC 7 cut(s) 257, 495, 504, 590, 593, 714, 766
BstV2I GAAGAC 4 cut(s) 32, 141, 519, 892
BstXI CCANNNNNNTGG 1 cut(s) 118
Bsu36I CCTNAGG 1 cut(s) 563
BsuRI GGCC 3 cut(s) 457, 741, 822
BtgZI GCGATG 2 cut(s) 456, 533
BtsCI GGATG 3 cut(s) 214, 367, 505
BtsI GCAGTG 1 cut(s) 667
BtsIMutI CAGTG 1 cut(s) 667
BveI ACCTGC 2 cut(s) 269, 549
Cac8I GCNNGC 4 cut(s) 268, 289, 490, 784
CfoI GCGC 4 cut(s) 266, 287, 488, 707
Cfr10I RCCGGY 1 cut(s) 266
Csp6I GTAC 1 cut(s) 280
CviAII CATG 4 cut(s) 329, 489, 695, 715
CviQI GTAC 1 cut(s) 280
DdeI CTNAG 1 cut(s) 563
DpnI GATC 2 cut(s) 242, 880
DpnII GATC 2 cut(s) 240, 878
Eam1104I CTCTTC 2 cut(s) 189, 260
EarI CTCTTC 2 cut(s) 189, 260
EciI GGCGGA 1 cut(s) 615
Eco130I CCWWGG 1 cut(s) 342
Eco57I CTGAAG 1 cut(s) 513
Eco81I CCTNAGG 1 cut(s) 563
EcoNI CCTNNNNNAGG 1 cut(s) 827
EcoRI GAATTC 2 cut(s) 161, 809
EcoT14I CCWWGG 1 cut(s) 342
EcoT22I ATGCAT 1 cut(s) 507
ErhI CCWWGG 1 cut(s) 342
FaeI CATG 4 cut(s) 332, 492, 698, 718
FaiI YATR 7 cut(s) 330, 471, 490, 558, 690, 696, 716
FalI AAGNNNNNCTT 2 cut(s) 186, 218
FaqI GGGAC 3 cut(s) 116, 217, 787
FatI CATG 4 cut(s) 328, 488, 694, 714
Fnu4HI GCNGC 8 cut(s) 271, 484, 493, 579, 582, 728, 780, 820
FokI GGATG 3 cut(s) 201, 354, 492
Fsp4HI GCNGC 8 cut(s) 271, 484, 493, 579, 582, 728, 780, 820
FspBI CTAG 1 cut(s) 135
GlaI GCGC 4 cut(s) 265, 286, 487, 706
GluI GCNGC 8 cut(s) 271, 484, 493, 579, 582, 728, 780, 820
GsuI CTGGAG 1 cut(s) 336
HaeII RGCGCY 1 cut(s) 288
HaeIII GGCC 3 cut(s) 457, 741, 822
HapII CCGG 1 cut(s) 267
HhaI GCGC 4 cut(s) 266, 287, 488, 707
Hin1II CATG 4 cut(s) 332, 492, 698, 718
Hin6I GCGC 4 cut(s) 264, 285, 486, 705
HinP1I GCGC 4 cut(s) 264, 285, 486, 705
HinfI GANTC 3 cut(s) 56, 547, 605
HpaII CCGG 1 cut(s) 267
HphI GGTGA 2 cut(s) 250, 542
Hpy166II GTNNAC 1 cut(s) 664
Hpy188I TCNGA 4 cut(s) 55, 479, 532, 867
Hpy188III TCNNGA 2 cut(s) 499, 882
Hpy8I GTNNAC 1 cut(s) 664
HpyAV CCTTC 2 cut(s) 382, 836
HpyF10VI GCNNNNNNNGC 7 cut(s) 270, 353, 489, 492, 704, 757, 788
HpyF3I CTNAG 1 cut(s) 563
Hsp92II CATG 4 cut(s) 332, 492, 698, 718
HspAI GCGC 4 cut(s) 264, 285, 486, 705
KroI GCCGGC 1 cut(s) 266
KroNI GCCGGC 1 cut(s) 268
Kzo9I GATC 2 cut(s) 240, 878
LmnI GCTCC 2 cut(s) 330, 352
Lsp1109I GCAGC 7 cut(s) 257, 495, 504, 590, 593, 714, 766
LweI GCATC 4 cut(s) 470, 492, 514, 800
MaeI CTAG 1 cut(s) 135
MaeIII GTNAC 2 cut(s) 314, 449
MalI GATC 2 cut(s) 242, 880
MboI GATC 2 cut(s) 240, 878
MboII GAAGA 9 cut(s) 34, 37, 141, 206, 247, 519, 600, 658, 897
MluCI AATT 4 cut(s) 161, 423, 809, 901
MlyI GAGTC 1 cut(s) 541
MmeI TCCRAC 2 cut(s) 99, 406
Mph1103I ATGCAT 1 cut(s) 507
MroNI GCCGGC 1 cut(s) 266
MseI TTAA 1 cut(s) 414
MslI CAYNNNNRTG 1 cut(s) 693
MspI CCGG 1 cut(s) 267
MwoI GCNNNNNNNGC 7 cut(s) 270, 353, 489, 492, 704, 757, 788
NaeI GCCGGC 1 cut(s) 268
NdeII GATC 2 cut(s) 240, 878
NgoMIV GCCGGC 1 cut(s) 266
NlaIII CATG 4 cut(s) 332, 492, 698, 718
NlaIV GGNNCC 1 cut(s) 348
NmeAIII GCCGAG 1 cut(s) 786
NmuCI GTSAC 2 cut(s) 314, 449
NsiI ATGCAT 1 cut(s) 507
NspI RCATGY 1 cut(s) 492
PaeI GCATGC 1 cut(s) 492
PaqCI CACCTGC 1 cut(s) 269
PdiI GCCGGC 1 cut(s) 268
PfeI GAWTC 2 cut(s) 56, 605
PkrI GCNGC 8 cut(s) 272, 485, 494, 580, 583, 729, 781, 821
PleI GAGTC 1 cut(s) 541
PpsI GAGTC 1 cut(s) 541
PspN4I GGNNCC 1 cut(s) 348
PstI CTGCAG 1 cut(s) 546
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
RseI CAYNNNNRTG 1 cut(s) 693
SaqAI TTAA 1 cut(s) 414
SatI GCNGC 8 cut(s) 271, 484, 493, 579, 582, 728, 780, 820
Sau3AI GATC 2 cut(s) 240, 878
SchI GAGTC 1 cut(s) 541
SfaNI GCATC 4 cut(s) 470, 492, 514, 800
SfcI CTRYAG 1 cut(s) 542
SmiMI CAYNNNNRTG 1 cut(s) 693
SmlI CTYRAG 2 cut(s) 743, 795
SmoI CTYRAG 2 cut(s) 743, 795
SphI GCATGC 1 cut(s) 492
Sse9I AATT 4 cut(s) 161, 423, 809, 901
SsiI CCGC 3 cut(s) 600, 632, 819
SspI AATATT 2 cut(s) 127, 871
SspMI CTAG 1 cut(s) 135
StyI CCWWGG 1 cut(s) 342
TaqI TCGA 1 cut(s) 159
TaqII GACCGA 1 cut(s) 200
TasI AATT 4 cut(s) 161, 423, 809, 901
TauI GCSGC 1 cut(s) 822
TfiI GAWTC 2 cut(s) 56, 605
Tru1I TTAA 1 cut(s) 414
Tru9I TTAA 1 cut(s) 414
TscAI CASTG 1 cut(s) 674
TseFI GTSAC 2 cut(s) 314, 449
TseI GCWGC 7 cut(s) 270, 483, 492, 578, 581, 727, 779
Tsp45I GTSAC 2 cut(s) 314, 449
TspDTI ATGAA 4 cut(s) 194, 289, 683, 802
TspRI CASTG 1 cut(s) 674
XagI CCTNNNNNAGG 1 cut(s) 827
XapI RAATTY 2 cut(s) 161, 809
XceI RCATGY 1 cut(s) 492
XspI CTAG 1 cut(s) 135
Zsp2I ATGCAT 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.