pycom16g26300

C1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014254
Physical Location & Seq
Reverse (-)
28702319 .. 28703482
1164 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g26300.1

Sequence Viewer

Length: 921 bp
ATGATGATGAGCAATAAACCCTTGAAGAAGACAAGAACTTTTCTCCTCAGGAGGTCAGAATCAACTGCCCCGCAATCCCAATCCCAACCCCAATCCCAAACCCAATCCCAATCCCAATCCCAACCCACAAAGCTGGATAATATTGTCCCTAGAAATAATAAGTCTCCTTTGCTCGAATTCCCCACATCTCCTCAACTGATATTTGGGGAAGAGATACTTCAGTTCGGTCATCCACAACACCCTCTGTCCCAAGTGATCCTGCCCGACCTCTTCACCTGCGCCGGCTGCAAGGAGTACGGCGCCGGCAAGAGGTTCATTTGCCAACAATGTGATTTTCAGCTCCATGATTTTTGTGCCTTGGCTCCTCCAGCTCTAAAGAACCATCCCTTTCACTTCCAACACCAACTTGTTTTCTATTCTAAAGCAGTTAAAGGAGGAATTGCACAATCCAAGTGCGATGTTTGTCACAGGCCAAGCAAAGGTTATGCTTTCAGATGCAGCGCATGCAGCTTCCAGATGCATCCTTGCTGTGCGATGTTGTCTTCAGAAATAAACCTACAGACTCACCCACATACCCTAAGGCTTTTACCAGCAGCAGCAAGTGGTGGTAGTGGCGGCGATTCTTCTGGTAGCAGTTTTGTTTGTGCGGAGTGCAGGAGGAAGAGGTCGGGCAGGGTTTACCACTGCAAGGTGTGTGATTACCATATTCATGCAGTTTGCGCCAAGAACATGATAAATGGGCTGCAAGAAAACGGCCTCAAGGGGCGAGAAAAGCCGAGTGTGTTGGGGACTGCTGCTCGCCTCGCATCTCAAGTCGTCATTGAATTCATCGGCGGCCTCATTGAGGGGCTTGGGGAAGGTGTTGCCGAAGTATTCGTTCAGAATATTGCCCGATCAGGAAGACCCAATGCCCCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2

Protein Analysis

307

Amino Acids

33.5

Weight (kDa)

9.14

Isoelectric Point (pI)

77.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C1_2 PF03107 76 - 119 5.8e-08 C1 domain
C1_2 PF03107 130 - 178 2.8e-06 C1 domain
C1_2 PF03107 188 - 241 5.1e-09 C1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20990
fragaria_vesca FvH4_5g26520
malus_domestica MD00G1031500.v1.1 MD06G1001400.v1.1
prunus_persica Prupe.5G002200_v2.0.a1
pyrus_communis pycom06g00150 pycom16g26300
rosa_chinensis RchiOBHm_Chr7g0217421
rosa_laevigata RLG00000002478
rosa_multiflora Rmu_sc0000145.1_g000014
rosa_roxburghii Rroxscaffold_3G00242090
rosa_rugosa Rorug07G0169000
rosa_samantha Rh7AG308700 Rh7BG299400 Rh7CG328100 Rh7DG308000
rosa_wichuraiana Rw7G026180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 284
Acc36I ACCTGC 1 cut(s) 284
AccB1I GGYRCC 1 cut(s) 299
AciI CCGC 4 cut(s) 71, 615, 647, 834
AclWI GGATC 1 cut(s) 250
AcsI RAATTY 2 cut(s) 176, 824
AcuI CTGAAG 2 cut(s) 203, 528
AcyI GRCGYC 1 cut(s) 300
AfaI GTAC 1 cut(s) 296
AfiI CCNNNNNNNGG 4 cut(s) 309, 479, 688, 844
AgsI TTSAA 2 cut(s) 25, 824
AluBI AGCT 4 cut(s) 133, 340, 371, 510
AluI AGCT 4 cut(s) 133, 340, 371, 510
Alw26I GTCTC 1 cut(s) 168
AlwI GGATC 1 cut(s) 250
AoxI GGCC 3 cut(s) 470, 754, 835
ApeKI GCWGC 7 cut(s) 285, 498, 507, 593, 596, 742, 794
ApoI RAATTY 2 cut(s) 176, 824
AspLEI GCGC 4 cut(s) 281, 302, 503, 722
AsuHPI GGTGA 2 cut(s) 265, 557
AxyI CCTNAGG 2 cut(s) 47, 578
BaeI ACNNNNGTAYC 2 cut(s) 206, 239
BanI GGYRCC 1 cut(s) 299
BbsI GAAGAC 3 cut(s) 35, 534, 907
BbvI GCAGC 7 cut(s) 272, 510, 519, 605, 608, 729, 781
BccI CCATC 1 cut(s) 390
BceAI ACGGC 2 cut(s) 313, 769
BcoDI GTCTC 1 cut(s) 168
BfaI CTAG 1 cut(s) 150
BfmI CTRYAG 1 cut(s) 557
BfoI RGCGCY 1 cut(s) 303
BfuAI ACCTGC 1 cut(s) 284
BisI GCNGC 9 cut(s) 286, 499, 508, 594, 597, 616, 743, 795, 835
BlsI GCNGC 9 cut(s) 287, 500, 509, 595, 598, 617, 744, 796, 836
BmiI GGNNCC 2 cut(s) 301, 363
BmsI GCATC 4 cut(s) 485, 507, 529, 815
BpiI GAAGAC 3 cut(s) 35, 534, 907
BpmI CTGGAG 1 cut(s) 351
BpuEI CTTGAG 2 cut(s) 743, 795
BsaHI GRCGYC 1 cut(s) 300
BsaJI CCNNGG 1 cut(s) 357
Bsc4I CCNNNNNNNGG 4 cut(s) 309, 479, 688, 844
Bse118I RCCGGY 2 cut(s) 281, 302
Bse21I CCTNAGG 2 cut(s) 47, 578
BseDI CCNNGG 1 cut(s) 357
BseGI GGATG 3 cut(s) 229, 382, 520
BseLI CCNNNNNNNGG 4 cut(s) 309, 479, 688, 844
BseMII CTCAG 1 cut(s) 61
BseRI GAGGAG 3 cut(s) 35, 180, 354
BseXI GCAGC 7 cut(s) 272, 510, 519, 605, 608, 729, 781
BsgI GTGCAG 1 cut(s) 673
BshFI GGCC 3 cut(s) 472, 756, 837
BshNI GGYRCC 1 cut(s) 299
BsiSI CCGG 2 cut(s) 282, 303
BslFI GGGAC 3 cut(s) 131, 232, 802
BslI CCNNNNNNNGG 4 cut(s) 309, 479, 688, 844
BsmAI GTCTC 1 cut(s) 168
BsmFI GGGAC 3 cut(s) 131, 232, 802
BsnI GGCC 3 cut(s) 472, 756, 837
Bsp143I GATC 2 cut(s) 255, 893
BspACI CCGC 4 cut(s) 71, 615, 647, 834
BspANI GGCC 3 cut(s) 472, 756, 837
BspCNI CTCAG 1 cut(s) 60
BspLI GGNNCC 2 cut(s) 301, 363
BspMI ACCTGC 1 cut(s) 284
BspPI GGATC 1 cut(s) 250
BspT107I GGYRCC 1 cut(s) 299
BsrFI RCCGGY 2 cut(s) 281, 302
BssAI RCCGGY 2 cut(s) 281, 302
BssECI CCNNGG 1 cut(s) 357
BssMI GATC 2 cut(s) 255, 893
BssNI GRCGYC 1 cut(s) 300
BssT1I CCWWGG 1 cut(s) 357
Bst6I CTCTTC 3 cut(s) 204, 275, 656
BstACI GRCGYC 1 cut(s) 300
BstAPI GCANNNNNTGC 1 cut(s) 504
BstC8I GCNNGC 4 cut(s) 283, 304, 505, 799
BstDEI CTNAG 2 cut(s) 47, 578
BstENI CCTNNNNNAGG 1 cut(s) 842
BstF5I GGATG 3 cut(s) 229, 382, 520
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 4 cut(s) 281, 302, 503, 722
BstKTI GATC 2 cut(s) 258, 896
BstMAI GTCTC 1 cut(s) 168
BstMBI GATC 2 cut(s) 255, 893
BstMWI GCNNNNNNNGC 7 cut(s) 285, 368, 504, 507, 719, 772, 803
BstNSI RCATGY 1 cut(s) 507
BstSFI CTRYAG 1 cut(s) 557
BstV1I GCAGC 7 cut(s) 272, 510, 519, 605, 608, 729, 781
BstV2I GAAGAC 3 cut(s) 35, 534, 907
BstXI CCANNNNNNTGG 1 cut(s) 133
Bsu36I CCTNAGG 2 cut(s) 47, 578
BsuRI GGCC 3 cut(s) 472, 756, 837
BtgZI GCGATG 2 cut(s) 471, 548
BtsCI GGATG 3 cut(s) 229, 382, 520
BtsI GCAGTG 1 cut(s) 682
BtsIMutI CAGTG 1 cut(s) 682
BveI ACCTGC 1 cut(s) 284
Cac8I GCNNGC 4 cut(s) 283, 304, 505, 799
CfoI GCGC 4 cut(s) 281, 302, 503, 722
Cfr10I RCCGGY 2 cut(s) 281, 302
Csp6I GTAC 1 cut(s) 295
CviAII CATG 4 cut(s) 344, 504, 710, 730
CviQI GTAC 1 cut(s) 295
DdeI CTNAG 2 cut(s) 47, 578
DinI GGCGCC 1 cut(s) 301
DpnI GATC 2 cut(s) 257, 895
DpnII GATC 2 cut(s) 255, 893
Eam1104I CTCTTC 3 cut(s) 204, 275, 656
EarI CTCTTC 3 cut(s) 204, 275, 656
Eco130I CCWWGG 1 cut(s) 357
Eco57I CTGAAG 2 cut(s) 203, 528
Eco81I CCTNAGG 2 cut(s) 47, 578
EcoNI CCTNNNNNAGG 1 cut(s) 842
EcoRI GAATTC 2 cut(s) 176, 824
EcoT14I CCWWGG 1 cut(s) 357
EcoT22I ATGCAT 1 cut(s) 522
EgeI GGCGCC 1 cut(s) 301
EheI GGCGCC 1 cut(s) 301
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 4 cut(s) 347, 507, 713, 733
FaiI YATR 7 cut(s) 345, 486, 505, 573, 705, 711, 731
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FaqI GGGAC 3 cut(s) 131, 232, 802
FatI CATG 4 cut(s) 343, 503, 709, 729
FauI CCCGC 1 cut(s) 78
Fnu4HI GCNGC 9 cut(s) 286, 499, 508, 594, 597, 616, 743, 795, 835
FokI GGATG 3 cut(s) 216, 369, 507
Fsp4HI GCNGC 9 cut(s) 286, 499, 508, 594, 597, 616, 743, 795, 835
FspBI CTAG 1 cut(s) 150
GlaI GCGC 4 cut(s) 280, 301, 502, 721
GluI GCNGC 9 cut(s) 286, 499, 508, 594, 597, 616, 743, 795, 835
GsuI CTGGAG 1 cut(s) 351
HaeII RGCGCY 1 cut(s) 303
HaeIII GGCC 3 cut(s) 472, 756, 837
HapII CCGG 2 cut(s) 282, 303
HhaI GCGC 4 cut(s) 281, 302, 503, 722
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 4 cut(s) 347, 507, 713, 733
Hin6I GCGC 4 cut(s) 279, 300, 501, 720
HinP1I GCGC 4 cut(s) 279, 300, 501, 720
HinfI GANTC 3 cut(s) 59, 562, 620
HpaII CCGG 2 cut(s) 282, 303
HphI GGTGA 2 cut(s) 265, 557
Hpy166II GTNNAC 1 cut(s) 679
Hpy188I TCNGA 4 cut(s) 58, 494, 547, 882
Hpy188III TCNNGA 3 cut(s) 49, 514, 897
Hpy8I GTNNAC 1 cut(s) 679
HpyAV CCTTC 1 cut(s) 851
HpyCH4V TGCA 9 cut(s) 288, 443, 498, 507, 520, 654, 687, 713, 745
HpyF10VI GCNNNNNNNGC 7 cut(s) 285, 368, 504, 507, 719, 772, 803
HpyF3I CTNAG 2 cut(s) 47, 578
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 4 cut(s) 347, 507, 713, 733
HspAI GCGC 4 cut(s) 279, 300, 501, 720
KasI GGCGCC 1 cut(s) 299
KroI GCCGGC 2 cut(s) 281, 302
KroNI GCCGGC 2 cut(s) 283, 304
Kzo9I GATC 2 cut(s) 255, 893
LmnI GCTCC 2 cut(s) 345, 367
Lsp1109I GCAGC 7 cut(s) 272, 510, 519, 605, 608, 729, 781
LweI GCATC 4 cut(s) 485, 507, 529, 815
MaeI CTAG 1 cut(s) 150
MaeIII GTNAC 1 cut(s) 464
MalI GATC 2 cut(s) 257, 895
MboI GATC 2 cut(s) 255, 893
MboII GAAGA 8 cut(s) 37, 40, 221, 262, 534, 615, 673, 912
MluCI AATT 4 cut(s) 176, 438, 824, 916
Mly113I GGCGCC 1 cut(s) 300
MlyI GAGTC 1 cut(s) 556
MmeI TCCRAC 1 cut(s) 421
Mph1103I ATGCAT 1 cut(s) 522
MroNI GCCGGC 2 cut(s) 281, 302
MseI TTAA 1 cut(s) 429
MslI CAYNNNNRTG 1 cut(s) 708
MspI CCGG 2 cut(s) 282, 303
MwoI GCNNNNNNNGC 7 cut(s) 285, 368, 504, 507, 719, 772, 803
NaeI GCCGGC 2 cut(s) 283, 304
NarI GGCGCC 1 cut(s) 300
NdeII GATC 2 cut(s) 255, 893
NgoMIV GCCGGC 2 cut(s) 281, 302
NlaIII CATG 4 cut(s) 347, 507, 713, 733
NlaIV GGNNCC 2 cut(s) 301, 363
NmeAIII GCCGAG 1 cut(s) 801
NmuCI GTSAC 1 cut(s) 464
NsiI ATGCAT 1 cut(s) 522
NspI RCATGY 1 cut(s) 507
PaeI GCATGC 1 cut(s) 507
PaqCI CACCTGC 1 cut(s) 284
PdiI GCCGGC 2 cut(s) 283, 304
PfeI GAWTC 2 cut(s) 59, 620
PkrI GCNGC 9 cut(s) 287, 500, 509, 595, 598, 617, 744, 796, 836
PleI GAGTC 1 cut(s) 556
PluTI GGCGCC 1 cut(s) 303
PpsI GAGTC 1 cut(s) 556
PspN4I GGNNCC 2 cut(s) 301, 363
RsaI GTAC 1 cut(s) 296
RsaNI GTAC 1 cut(s) 295
RseI CAYNNNNRTG 1 cut(s) 708
SaqAI TTAA 1 cut(s) 429
SatI GCNGC 9 cut(s) 286, 499, 508, 594, 597, 616, 743, 795, 835
Sau3AI GATC 2 cut(s) 255, 893
SchI GAGTC 1 cut(s) 556
SfaNI GCATC 4 cut(s) 485, 507, 529, 815
SfcI CTRYAG 1 cut(s) 557
SfoI GGCGCC 1 cut(s) 301
SmiMI CAYNNNNRTG 1 cut(s) 708
SmlI CTYRAG 2 cut(s) 758, 810
SmoI CTYRAG 2 cut(s) 758, 810
SphI GCATGC 1 cut(s) 507
Sse9I AATT 4 cut(s) 176, 438, 824, 916
SsiI CCGC 4 cut(s) 71, 615, 647, 834
SspDI GGCGCC 1 cut(s) 299
SspI AATATT 2 cut(s) 142, 886
SspMI CTAG 1 cut(s) 150
StyI CCWWGG 1 cut(s) 357
TaqI TCGA 1 cut(s) 174
TaqII GACCGA 1 cut(s) 215
TasI AATT 4 cut(s) 176, 438, 824, 916
TauI GCSGC 2 cut(s) 618, 837
TfiI GAWTC 2 cut(s) 59, 620
Tru1I TTAA 1 cut(s) 429
Tru9I TTAA 1 cut(s) 429
TscAI CASTG 1 cut(s) 689
TseFI GTSAC 1 cut(s) 464
TseI GCWGC 7 cut(s) 285, 498, 507, 593, 596, 742, 794
Tsp45I GTSAC 1 cut(s) 464
TspDTI ATGAA 3 cut(s) 304, 698, 817
TspRI CASTG 1 cut(s) 689
XagI CCTNNNNNAGG 1 cut(s) 842
XapI RAATTY 2 cut(s) 176, 824
XceI RCATGY 1 cut(s) 507
XspI CTAG 1 cut(s) 150
Zsp2I ATGCAT 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.