pycom06g00150

C1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0014254
Physical Location & Seq
Forward (+)
173198 .. 176120
2923 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g00150.2

Sequence Viewer

Length: 900 bp
ATGATGATAAGCAATAAACCCCCGAGGAAGACAACTTTTCTCCTCAAGAGGTCAGAATCAACTCCCCCCCAATCCCAACCCACAAATTTAGATAATATTGTCCCTAGAAATAACAAGTCTCCTTTGCTCGAATTCCCCACATCTCAGCTGATATTTGGGGAAGAGAGACTTCATTTCGGTCATCCACAACACCCCCTGTCCCAAGTGATCCTGCCCGACCTCTTCACCTGCGCAGGCTGCAAGGAGGACGGCGCCGGCAAGAGGTTCATTTGTCAACTATGTCAATTTCAGCTGCATGATTTTTGTGCCTTGGCTCCTCCAGCTCTAAAGAACCATCCCTTCCACATCCAACACCAACTTGTTTTCTATTCTAAAGCAGTTAAGGGAGGAATTGCACAATCCAAGTGCAATATTTGTCACAAGCCAAGCAAAGGTTATGCTTTCAGATGCAGCGCATGCAGCTTCCAGATGCATCCTTGCTGTGCGATGCTGTCATCAGAAATAAACCTCCAGACTCACCCACATACCCTAAGACTTTTACCAGCAGCAGCAGCAAGTGGTACTGGTAGTAGTGGCGGAGATTTTTCTACTAGGAGTTTTGTTTGTGGGGAGTGCAGGAGGAGGAGGTCGGGCAGGGTTTACCACTGCACGGTGTGCGATTACCATCTGCATGCAGTGTGCGCTAAGAACATGATAAATGGGTTGCAAGAGAACGGCCTCAAGGGACGTGAAAAGCCGAGTGTGTTAGGGACTGCTGCTCGCCTCGCGTCTCAAGTTGTCACTGAATTCGTCGGCGGCATCATTGAGGGGCTTGGGGAAAGTGTTGCTGAAGTCTTGGTTCAAGATATTGCCAGGTCAGGAAGACCAAATGCCCGCCGATCATCCAATTCTCCTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2

Protein Analysis

300

Amino Acids

32.72

Weight (kDa)

9.17

Isoelectric Point (pI)

67.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20990
fragaria_vesca FvH4_5g26520
malus_domestica MD00G1031500.v1.1 MD06G1001400.v1.1
prunus_persica Prupe.5G002200_v2.0.a1
pyrus_communis pycom06g00150 pycom16g26300
rosa_chinensis RchiOBHm_Chr7g0217421
rosa_laevigata RLG00000002478
rosa_multiflora Rmu_sc0000145.1_g000014
rosa_roxburghii Rroxscaffold_3G00242090
rosa_rugosa Rorug07G0169000
rosa_samantha Rh7AG308700 Rh7BG299400 Rh7CG328100 Rh7DG308000
rosa_wichuraiana Rw7G026180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 236
Acc16I TGCGCA 1 cut(s) 232
Acc36I ACCTGC 1 cut(s) 236
AccB1I GGYRCC 1 cut(s) 251
AccII CGCG 1 cut(s) 767
AciI CCGC 3 cut(s) 576, 795, 874
AclWI GGATC 1 cut(s) 202
AcsI RAATTY 3 cut(s) 85, 131, 785
AcuI CTGAAG 1 cut(s) 849
AcyI GRCGYC 1 cut(s) 252
AdeI CACNNNGTG 1 cut(s) 654
AfaI GTAC 1 cut(s) 562
AfiI CCNNNNNNNGG 3 cut(s) 261, 431, 649
AgsI TTSAA 1 cut(s) 842
AjiI CACGTC 1 cut(s) 728
AjnI CCWGG 1 cut(s) 851
AluBI AGCT 4 cut(s) 148, 292, 323, 462
AluI AGCT 4 cut(s) 148, 292, 323, 462
Alw26I GTCTC 3 cut(s) 123, 160, 774
AlwI GGATC 1 cut(s) 202
Ama87I CYCGRG 1 cut(s) 22
AoxI GGCC 1 cut(s) 715
ApeKI GCWGC 8 cut(s) 237, 292, 450, 459, 545, 548, 551, 755
ApoI RAATTY 3 cut(s) 85, 131, 785
AspLEI GCGC 4 cut(s) 233, 254, 455, 683
AsuHPI GGTGA 2 cut(s) 217, 509
AvaI CYCGRG 1 cut(s) 22
BanI GGYRCC 1 cut(s) 251
BbsI GAAGAC 2 cut(s) 35, 868
BbvI GCAGC 8 cut(s) 224, 279, 462, 471, 557, 560, 563, 742
BccI CCATC 2 cut(s) 342, 672
BceAI ACGGC 2 cut(s) 265, 730
BciT130I CCWGG 1 cut(s) 853
BcoDI GTCTC 3 cut(s) 123, 160, 774
BfaI CTAG 2 cut(s) 105, 591
BfoI RGCGCY 1 cut(s) 255
BfuAI ACCTGC 1 cut(s) 236
BisI GCNGC 9 cut(s) 238, 293, 451, 460, 546, 549, 552, 756, 796
BlsI GCNGC 9 cut(s) 239, 294, 452, 461, 547, 550, 553, 757, 797
Bme1390I CCNGG 1 cut(s) 853
BmeT110I CYCGRG 1 cut(s) 22
BmgBI CACGTC 1 cut(s) 728
BmiI GGNNCC 2 cut(s) 253, 315
BmrFI CCNGG 1 cut(s) 853
BmsI GCATC 5 cut(s) 437, 459, 477, 481, 807
BpiI GAAGAC 2 cut(s) 35, 868
BpmI CTGGAG 2 cut(s) 303, 494
BpuEI CTTGAG 3 cut(s) 29, 704, 756
BsaBI GATNNNNATC 1 cut(s) 663
BsaHI GRCGYC 1 cut(s) 252
BsaJI CCNNGG 2 cut(s) 23, 309
Bsc4I CCNNNNNNNGG 3 cut(s) 261, 431, 649
Bse118I RCCGGY 1 cut(s) 254
Bse1I ACTGG 1 cut(s) 568
Bse8I GATNNNNATC 1 cut(s) 663
BseBI CCWGG 1 cut(s) 853
BseDI CCNNGG 2 cut(s) 23, 309
BseGI GGATG 5 cut(s) 181, 334, 345, 472, 881
BseJI GATNNNNATC 1 cut(s) 663
BseLI CCNNNNNNNGG 3 cut(s) 261, 431, 649
BseMII CTCAG 1 cut(s) 158
BseNI ACTGG 1 cut(s) 568
BseRI GAGGAG 4 cut(s) 32, 306, 634, 637
BseXI GCAGC 8 cut(s) 224, 279, 462, 471, 557, 560, 563, 742
BsgI GTGCAG 2 cut(s) 631, 634
Bsh1236I CGCG 1 cut(s) 767
BshFI GGCC 1 cut(s) 717
BshNI GGYRCC 1 cut(s) 251
BsiHKCI CYCGRG 1 cut(s) 22
BsiSI CCGG 1 cut(s) 255
BslFI GGGAC 4 cut(s) 86, 184, 738, 763
BslI CCNNNNNNNGG 3 cut(s) 261, 431, 649
BsmAI GTCTC 3 cut(s) 123, 160, 774
BsmBI CGTCTC 1 cut(s) 774
BsmFI GGGAC 4 cut(s) 86, 184, 738, 763
BsnI GGCC 1 cut(s) 717
BsoBI CYCGRG 1 cut(s) 22
Bsp143I GATC 2 cut(s) 207, 878
BspACI CCGC 3 cut(s) 576, 795, 874
BspANI GGCC 1 cut(s) 717
BspCNI CTCAG 1 cut(s) 157
BspFNI CGCG 1 cut(s) 767
BspLI GGNNCC 2 cut(s) 253, 315
BspMI ACCTGC 1 cut(s) 236
BspPI GGATC 1 cut(s) 202
BspT107I GGYRCC 1 cut(s) 251
BsrFI RCCGGY 1 cut(s) 254
BsrI ACTGG 1 cut(s) 568
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 2 cut(s) 23, 309
BssMI GATC 2 cut(s) 207, 878
BssNI GRCGYC 1 cut(s) 252
BssT1I CCWWGG 1 cut(s) 309
Bst2UI CCWGG 1 cut(s) 853
Bst4CI ACNGT 1 cut(s) 652
Bst6I CTCTTC 2 cut(s) 156, 227
BstACI GRCGYC 1 cut(s) 252
BstAPI GCANNNNNTGC 2 cut(s) 456, 654
BstC8I GCNNGC 6 cut(s) 235, 256, 457, 672, 760, 874
BstDEI CTNAG 3 cut(s) 144, 530, 684
BstF5I GGATG 5 cut(s) 181, 334, 345, 472, 881
BstFNI CGCG 1 cut(s) 767
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 4 cut(s) 233, 254, 455, 683
BstKTI GATC 2 cut(s) 210, 881
BstMAI GTCTC 3 cut(s) 123, 160, 774
BstMBI GATC 2 cut(s) 207, 878
BstMWI GCNNNNNNNGC 8 cut(s) 237, 320, 456, 459, 551, 654, 680, 764
BstNI CCWGG 1 cut(s) 853
BstNSI RCATGY 2 cut(s) 459, 674
BstSCI CCNGG 1 cut(s) 851
BstUI CGCG 1 cut(s) 767
BstV1I GCAGC 8 cut(s) 224, 279, 462, 471, 557, 560, 563, 742
BstV2I GAAGAC 2 cut(s) 35, 868
BsuRI GGCC 1 cut(s) 717
BtgZI GCGATG 1 cut(s) 500
BtrI CACGTC 1 cut(s) 728
BtsCI GGATG 5 cut(s) 181, 334, 345, 472, 881
BtsI GCAGTG 2 cut(s) 643, 681
BtsIMutI CAGTG 3 cut(s) 643, 681, 780
BveI ACCTGC 1 cut(s) 236
Cac8I GCNNGC 6 cut(s) 235, 256, 457, 672, 760, 874
CfoI GCGC 4 cut(s) 233, 254, 455, 683
Cfr10I RCCGGY 1 cut(s) 254
CseI GACGC 1 cut(s) 756
Csp6I GTAC 1 cut(s) 561
CviAII CATG 4 cut(s) 296, 456, 671, 691
CviQI GTAC 1 cut(s) 561
DdeI CTNAG 3 cut(s) 144, 530, 684
DinI GGCGCC 1 cut(s) 253
DpnI GATC 2 cut(s) 209, 880
DpnII GATC 2 cut(s) 207, 878
DraIII CACNNNGTG 1 cut(s) 654
Eam1104I CTCTTC 2 cut(s) 156, 227
EarI CTCTTC 2 cut(s) 156, 227
EciI GGCGGA 1 cut(s) 591
Eco130I CCWWGG 1 cut(s) 309
Eco57I CTGAAG 1 cut(s) 849
Eco88I CYCGRG 1 cut(s) 22
EcoRI GAATTC 2 cut(s) 131, 785
EcoRII CCWGG 1 cut(s) 851
EcoT14I CCWWGG 1 cut(s) 309
EcoT22I ATGCAT 1 cut(s) 474
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 309
Esp3I CGTCTC 1 cut(s) 774
FaeI CATG 4 cut(s) 299, 459, 674, 694
FaiI YATR 7 cut(s) 280, 297, 438, 457, 525, 672, 692
FalI AAGNNNNNCTT 2 cut(s) 153, 185
FaqI GGGAC 4 cut(s) 86, 184, 738, 763
FatI CATG 4 cut(s) 295, 455, 670, 690
FauI CCCGC 1 cut(s) 881
Fnu4HI GCNGC 9 cut(s) 238, 293, 451, 460, 546, 549, 552, 756, 796
FokI GGATG 5 cut(s) 168, 321, 332, 459, 868
Fsp4HI GCNGC 9 cut(s) 238, 293, 451, 460, 546, 549, 552, 756, 796
FspBI CTAG 2 cut(s) 105, 591
FspI TGCGCA 1 cut(s) 232
GlaI GCGC 4 cut(s) 232, 253, 454, 682
GluI GCNGC 9 cut(s) 238, 293, 451, 460, 546, 549, 552, 756, 796
GsuI CTGGAG 2 cut(s) 303, 494
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 1 cut(s) 717
HapII CCGG 1 cut(s) 255
HgaI GACGC 1 cut(s) 756
HhaI GCGC 4 cut(s) 233, 254, 455, 683
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 4 cut(s) 299, 459, 674, 694
Hin6I GCGC 4 cut(s) 231, 252, 453, 681
HinP1I GCGC 4 cut(s) 231, 252, 453, 681
HincII GTYRAC 1 cut(s) 275
HindII GTYRAC 1 cut(s) 275
HinfI GANTC 2 cut(s) 56, 514
HpaII CCGG 1 cut(s) 255
HphI GGTGA 2 cut(s) 217, 509
Hpy166II GTNNAC 2 cut(s) 275, 640
Hpy188I TCNGA 3 cut(s) 55, 446, 499
Hpy188III TCNNGA 6 cut(s) 46, 466, 511, 842, 858, 893
Hpy8I GTNNAC 2 cut(s) 275, 640
Hpy99I CGWCG 1 cut(s) 794
HpyAV CCTTC 1 cut(s) 349
HpyCH4III ACNGT 1 cut(s) 652
HpyCH4IV ACGT 1 cut(s) 727
HpyF10VI GCNNNNNNNGC 8 cut(s) 237, 320, 456, 459, 551, 654, 680, 764
HpyF3I CTNAG 3 cut(s) 144, 530, 684
HpySE526I ACGT 1 cut(s) 727
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 4 cut(s) 299, 459, 674, 694
HspAI GCGC 4 cut(s) 231, 252, 453, 681
KasI GGCGCC 1 cut(s) 251
KroI GCCGGC 1 cut(s) 254
KroNI GCCGGC 1 cut(s) 256
Kzo9I GATC 2 cut(s) 207, 878
LmnI GCTCC 1 cut(s) 319
Lsp1109I GCAGC 8 cut(s) 224, 279, 462, 471, 557, 560, 563, 742
LweI GCATC 5 cut(s) 437, 459, 477, 481, 807
MaeI CTAG 2 cut(s) 105, 591
MaeII ACGT 1 cut(s) 727
MaeIII GTNAC 2 cut(s) 416, 778
MalI GATC 2 cut(s) 209, 880
MboI GATC 2 cut(s) 207, 878
MboII GAAGA 4 cut(s) 40, 173, 214, 873
MluCI AATT 6 cut(s) 85, 131, 284, 390, 785, 886
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 1 cut(s) 508
MmeI TCCRAC 1 cut(s) 373
Mph1103I ATGCAT 1 cut(s) 474
MroNI GCCGGC 1 cut(s) 254
MseI TTAA 1 cut(s) 381
MslI CAYNNNNRTG 1 cut(s) 669
MspA1I CMGCKG 2 cut(s) 148, 292
MspI CCGG 1 cut(s) 255
MspR9I CCNGG 1 cut(s) 853
MvaI CCWGG 1 cut(s) 853
MvnI CGCG 1 cut(s) 767
MwoI GCNNNNNNNGC 8 cut(s) 237, 320, 456, 459, 551, 654, 680, 764
NaeI GCCGGC 1 cut(s) 256
NarI GGCGCC 1 cut(s) 252
NdeII GATC 2 cut(s) 207, 878
NgoMIV GCCGGC 1 cut(s) 254
NlaIII CATG 4 cut(s) 299, 459, 674, 694
NlaIV GGNNCC 2 cut(s) 253, 315
NmeAIII GCCGAG 1 cut(s) 762
NmuCI GTSAC 2 cut(s) 416, 778
NsbI TGCGCA 1 cut(s) 232
NsiI ATGCAT 1 cut(s) 474
NspI RCATGY 2 cut(s) 459, 674
PaeI GCATGC 2 cut(s) 459, 674
PaqCI CACCTGC 1 cut(s) 236
PdiI GCCGGC 1 cut(s) 256
PfeI GAWTC 1 cut(s) 56
PkrI GCNGC 9 cut(s) 239, 294, 452, 461, 547, 550, 553, 757, 797
PleI GAGTC 1 cut(s) 508
PluTI GGCGCC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 508
Psp6I CCWGG 1 cut(s) 851
PspGI CCWGG 1 cut(s) 851
PspN4I GGNNCC 2 cut(s) 253, 315
PvuII CAGCTG 2 cut(s) 148, 292
RsaI GTAC 1 cut(s) 562
RsaNI GTAC 1 cut(s) 561
RseI CAYNNNNRTG 1 cut(s) 669
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 9 cut(s) 238, 293, 451, 460, 546, 549, 552, 756, 796
Sau3AI GATC 2 cut(s) 207, 878
SchI GAGTC 1 cut(s) 508
ScrFI CCNGG 1 cut(s) 853
SfaNI GCATC 5 cut(s) 437, 459, 477, 481, 807
SfoI GGCGCC 1 cut(s) 253
SmiMI CAYNNNNRTG 1 cut(s) 669
SmlI CTYRAG 3 cut(s) 44, 719, 771
SmoI CTYRAG 3 cut(s) 44, 719, 771
SphI GCATGC 2 cut(s) 459, 674
Sse9I AATT 6 cut(s) 85, 131, 284, 390, 785, 886
SsiI CCGC 3 cut(s) 576, 795, 874
SspDI GGCGCC 1 cut(s) 251
SspI AATATT 2 cut(s) 97, 412
SspMI CTAG 2 cut(s) 105, 591
StyD4I CCNGG 1 cut(s) 851
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 1 cut(s) 652
TaiI ACGT 1 cut(s) 730
TaqI TCGA 1 cut(s) 129
TaqII GACCGA 1 cut(s) 167
TasI AATT 6 cut(s) 85, 131, 284, 390, 785, 886
TauI GCSGC 1 cut(s) 798
TfiI GAWTC 1 cut(s) 56
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TscAI CASTG 3 cut(s) 650, 681, 787
TseFI GTSAC 2 cut(s) 416, 778
TseI GCWGC 8 cut(s) 237, 292, 450, 459, 545, 548, 551, 755
Tsp45I GTSAC 2 cut(s) 416, 778
TspDTI ATGAA 2 cut(s) 161, 256
TspRI CASTG 3 cut(s) 650, 681, 787
XapI RAATTY 3 cut(s) 85, 131, 785
XceI RCATGY 2 cut(s) 459, 674
XspI CTAG 2 cut(s) 105, 591
Zsp2I ATGCAT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.