Rmu_sc0000145.1_g000014

C1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000145.1
OG0014254
Physical Location & Seq
Reverse (-)
89530 .. 91224
1695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000145.1_g000014.1.cds

Sequence Viewer

Length: 879 bp
atgatgatgagcaacaacaacaaacccaccttgaagaagacaagaaccttttcccccaagaaatcagattcaatgcaaatccccacagctcaacccatggaatttgtccctaaaaggtgtgcttcgctaaagtttcctgcctctcctcaacttgttttgggggaggagatgctccatttcagtcacccacaacatcctctttcccatgtgaacttaccggacctcttcacctgcgctggctgtaaagagtacggcgcaggcaagaggtacacttgtcaacgatgtgattatcaacttcatgatttttgtgcctcagctcctccagttctaaagagtcatcccttccattgccaacaccaacttgctttctattctaaaccagtaaaaggagggattgcacaatcgaagtgtgatgtttgcagcaagcccatcaaaggctatgctttcagatgcggcgcaggttgtttccagatgcatccttgctgcgccatgctatcttcggaaatcagcttgccatatctccatccccataccctaagacttttacctgcatcaaaaagtggcagcgatccgagtttcgtgtgtggtgagtgcaggaggaagaggtcgggcaccagagtgtaccattgcaccgtctgtgactaccatcttcatgcggtatgtgcaaagaatgtgatcaatggacttcaagaaaatggcatcaagaacctagaaaagcccagcatgttggggactgcagctcgcctggcatctcaagtggtcatcgagttccttggggggttgatggaggggcttggggaaggtgttgcagaagtctttgtccaaaatgtcaccagatcaggaagaggcaatggtagaggaagaaatcctactccttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2

Protein Analysis

292

Amino Acids

32.07

Weight (kDa)

9.21

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20990
fragaria_vesca FvH4_5g26520
malus_domestica MD00G1031500.v1.1 MD06G1001400.v1.1
prunus_persica Prupe.5G002200_v2.0.a1
pyrus_communis pycom06g00150 pycom16g26300
rosa_chinensis RchiOBHm_Chr7g0217421
rosa_laevigata RLG00000002478
rosa_multiflora Rmu_sc0000145.1_g000014
rosa_roxburghii Rroxscaffold_3G00242090
rosa_rugosa Rorug07G0169000
rosa_samantha Rh7AG308700 Rh7BG299400 Rh7CG328100 Rh7DG308000
rosa_wichuraiana Rw7G026180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 239
Acc36I ACCTGC 3 cut(s) 239, 449, 556
AccB1I GGYRCC 1 cut(s) 611
AciI CCGC 2 cut(s) 453, 656
AclWI GGATC 1 cut(s) 563
AcsI RAATTY 1 cut(s) 101
AfaI GTAC 3 cut(s) 251, 269, 623
AfiI CCNNNNNNNGG 2 cut(s) 386, 434
AgsI TTSAA 3 cut(s) 34, 72, 689
AjnI CCWGG 1 cut(s) 744
AleI CACNNNNGTG 1 cut(s) 617
AluBI AGCT 4 cut(s) 89, 317, 510, 740
AluI AGCT 4 cut(s) 89, 317, 510, 740
AlwI GGATC 1 cut(s) 563
ApeKI GCWGC 4 cut(s) 420, 483, 564, 737
ApoI RAATTY 1 cut(s) 101
Asp700I GAANNNNTTC 1 cut(s) 49
AspLEI GCGC 4 cut(s) 236, 257, 458, 488
AspS9I GGNCC 1 cut(s) 220
AsuHPI GGTGA 4 cut(s) 176, 220, 599, 823
AvaII GGWCC 1 cut(s) 220
BaeGI GKGCMC 1 cut(s) 614
BaeI ACNNNNGTAYC 2 cut(s) 259, 292
BanI GGYRCC 1 cut(s) 611
BarI GAAGNNNNNNTAC 1 cut(s) 853
BbsI GAAGAC 1 cut(s) 44
BbvCI CCTCAGC 1 cut(s) 313
BbvI GCAGC 4 cut(s) 432, 470, 576, 749
BccI CCATC 4 cut(s) 437, 531, 654, 778
BceAI ACGGC 1 cut(s) 268
BciT130I CCWGG 1 cut(s) 746
BclI TGATCA 1 cut(s) 675
BfaI CTAG 1 cut(s) 710
BfmI CTRYAG 1 cut(s) 735
BfuAI ACCTGC 3 cut(s) 239, 449, 556
BisI GCNGC 5 cut(s) 421, 454, 484, 565, 738
BlsI GCNGC 5 cut(s) 422, 455, 485, 566, 739
Bme1390I CCNGG 1 cut(s) 746
Bme18I GGWCC 1 cut(s) 220
BmgT120I GGNCC 1 cut(s) 220
BmiI GGNNCC 1 cut(s) 613
BmrFI CCNGG 1 cut(s) 746
BmsI GCATC 7 cut(s) 159, 440, 462, 484, 560, 708, 758
BpiI GAAGAC 1 cut(s) 44
BpmI CTGGAG 1 cut(s) 306
Bpu10I CCTNAGC 1 cut(s) 313
BpuEI CTTGAG 1 cut(s) 738
BsaJI CCNNGG 2 cut(s) 96, 772
BsaWI WCCGGW 1 cut(s) 217
Bsc4I CCNNNNNNNGG 2 cut(s) 386, 434
Bse1I ACTGG 2 cut(s) 323, 380
Bse3DI GCAATG 3 cut(s) 346, 625, 856
BseBI CCWGG 1 cut(s) 746
BseDI CCNNGG 2 cut(s) 96, 772
BseGI GGATG 4 cut(s) 193, 337, 475, 523
BseLI CCNNNNNNNGG 2 cut(s) 386, 434
BseMI GCAATG 3 cut(s) 346, 625, 856
BseMII CTCAG 1 cut(s) 327
BseNI ACTGG 2 cut(s) 323, 380
BseRI GAGGAG 3 cut(s) 135, 179, 309
BseSI GKGCMC 1 cut(s) 614
BseXI GCAGC 4 cut(s) 432, 470, 576, 749
BseYI CCCAGC 1 cut(s) 719
BsgI GTGCAG 1 cut(s) 613
BshNI GGYRCC 1 cut(s) 611
BsiSI CCGG 1 cut(s) 218
BslFI GGGAC 2 cut(s) 92, 745
BslI CCNNNNNNNGG 2 cut(s) 386, 434
BsmFI GGGAC 2 cut(s) 92, 745
Bsp1286I GDGCHC 1 cut(s) 614
Bsp143I GATC 3 cut(s) 568, 675, 836
Bsp19I CCATGG 1 cut(s) 96
BspACI CCGC 2 cut(s) 453, 656
BspCNI CTCAG 1 cut(s) 326
BspHI TCATGA 1 cut(s) 298
BspLI GGNNCC 1 cut(s) 613
BspMAI CTGCAG 1 cut(s) 739
BspMI ACCTGC 3 cut(s) 239, 449, 556
BspPI GGATC 1 cut(s) 563
BspT107I GGYRCC 1 cut(s) 611
BsrDI GCAATG 3 cut(s) 346, 625, 856
BsrI ACTGG 2 cut(s) 323, 380
BssECI CCNNGG 2 cut(s) 96, 772
BssMI GATC 3 cut(s) 568, 675, 836
BssT1I CCWWGG 2 cut(s) 96, 772
Bst2UI CCWGG 1 cut(s) 746
Bst4CI ACNGT 1 cut(s) 634
Bst6I CTCTTC 3 cut(s) 230, 596, 838
BstC8I GCNNGC 5 cut(s) 238, 259, 425, 512, 742
BstDEI CTNAG 2 cut(s) 313, 536
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 4 cut(s) 193, 337, 475, 523
BstHHI GCGC 4 cut(s) 236, 257, 458, 488
BstKTI GATC 3 cut(s) 571, 678, 839
BstMBI GATC 3 cut(s) 568, 675, 836
BstMWI GCNNNNNNNGC 2 cut(s) 662, 746
BstNI CCWGG 1 cut(s) 746
BstNSI RCATGY 1 cut(s) 727
BstSCI CCNGG 1 cut(s) 744
BstSFI CTRYAG 1 cut(s) 735
BstSLI GKGCMC 1 cut(s) 614
BstV1I GCAGC 4 cut(s) 432, 470, 576, 749
BstV2I GAAGAC 1 cut(s) 44
BstXI CCANNNNNNTGG 1 cut(s) 727
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 4 cut(s) 193, 337, 475, 523
BveI ACCTGC 3 cut(s) 239, 449, 556
Cac8I GCNNGC 5 cut(s) 238, 259, 425, 512, 742
CciI TCATGA 1 cut(s) 298
CfoI GCGC 4 cut(s) 236, 257, 458, 488
Cfr13I GGNCC 1 cut(s) 220
Csp6I GTAC 3 cut(s) 250, 268, 622
CviAII CATG 6 cut(s) 97, 206, 299, 490, 653, 724
CviJI RGCY 9 cut(s) 89, 240, 317, 427, 438, 510, 718, 740, 793
CviKI_1 RGCY 9 cut(s) 89, 240, 317, 427, 438, 510, 718, 740, 793
CviQI GTAC 3 cut(s) 250, 268, 622
DdeI CTNAG 2 cut(s) 313, 536
DpnI GATC 3 cut(s) 570, 677, 838
DpnII GATC 3 cut(s) 568, 675, 836
Eam1104I CTCTTC 3 cut(s) 230, 596, 838
EarI CTCTTC 3 cut(s) 230, 596, 838
Eco130I CCWWGG 2 cut(s) 96, 772
Eco47I GGWCC 1 cut(s) 220
EcoRII CCWGG 1 cut(s) 744
EcoT14I CCWWGG 2 cut(s) 96, 772
EcoT22I ATGCAT 1 cut(s) 477
ErhI CCWWGG 2 cut(s) 96, 772
FaeI CATG 6 cut(s) 100, 209, 302, 493, 656, 727
FalI AAGNNNNNCTT 2 cut(s) 106, 138
FaqI GGGAC 2 cut(s) 92, 745
FatI CATG 6 cut(s) 96, 205, 298, 489, 652, 723
FbaI TGATCA 1 cut(s) 675
Fnu4HI GCNGC 5 cut(s) 421, 454, 484, 565, 738
FokI GGATG 4 cut(s) 180, 324, 462, 510
Fsp4HI GCNGC 5 cut(s) 421, 454, 484, 565, 738
FspBI CTAG 1 cut(s) 710
GlaI GCGC 4 cut(s) 235, 256, 457, 487
GluI GCNGC 5 cut(s) 421, 454, 484, 565, 738
GsaI CCCAGC 1 cut(s) 723
GsuI CTGGAG 1 cut(s) 306
HapII CCGG 1 cut(s) 218
HhaI GCGC 4 cut(s) 236, 257, 458, 488
Hin1II CATG 6 cut(s) 100, 209, 302, 493, 656, 727
Hin6I GCGC 4 cut(s) 234, 255, 456, 486
HinP1I GCGC 4 cut(s) 234, 255, 456, 486
HincII GTYRAC 1 cut(s) 278
HindII GTYRAC 1 cut(s) 278
HinfI GANTC 2 cut(s) 68, 334
HpaII CCGG 1 cut(s) 218
HphI GGTGA 4 cut(s) 176, 220, 599, 823
Hpy166II GTNNAC 4 cut(s) 211, 270, 278, 622
Hpy188I TCNGA 4 cut(s) 67, 449, 502, 573
Hpy188III TCNNGA 5 cut(s) 299, 469, 689, 703, 840
Hpy8I GTNNAC 4 cut(s) 211, 270, 278, 622
HpyAV CCTTC 2 cut(s) 352, 794
HpyCH4III ACNGT 1 cut(s) 634
HpyF10VI GCNNNNNNNGC 2 cut(s) 662, 746
HpyF3I CTNAG 2 cut(s) 313, 536
Hsp92II CATG 6 cut(s) 100, 209, 302, 493, 656, 727
HspAI GCGC 4 cut(s) 234, 255, 456, 486
Ksp22I TGATCA 1 cut(s) 675
Kzo9I GATC 3 cut(s) 568, 675, 836
LmnI GCTCC 2 cut(s) 177, 322
Lsp1109I GCAGC 4 cut(s) 432, 470, 576, 749
LweI GCATC 7 cut(s) 159, 440, 462, 484, 560, 708, 758
MaeI CTAG 1 cut(s) 710
MaeIII GTNAC 3 cut(s) 182, 638, 829
MalI GATC 3 cut(s) 570, 677, 838
MboI GATC 3 cut(s) 568, 675, 836
MboII GAAGA 8 cut(s) 46, 49, 217, 489, 613, 641, 855, 873
MhlI GDGCHC 1 cut(s) 614
MluCI AATT 1 cut(s) 101
MlyI GAGTC 1 cut(s) 343
Mph1103I ATGCAT 1 cut(s) 477
MroXI GAANNNNTTC 1 cut(s) 49
MslI CAYNNNNRTG 2 cut(s) 617, 651
MspI CCGG 1 cut(s) 218
MspR9I CCNGG 1 cut(s) 746
MvaI CCWGG 1 cut(s) 746
MwoI GCNNNNNNNGC 2 cut(s) 662, 746
NcoI CCATGG 1 cut(s) 96
NdeII GATC 3 cut(s) 568, 675, 836
NlaIII CATG 6 cut(s) 100, 209, 302, 493, 656, 727
NlaIV GGNNCC 1 cut(s) 613
NmuCI GTSAC 3 cut(s) 182, 638, 829
NsiI ATGCAT 1 cut(s) 477
NspI RCATGY 1 cut(s) 727
OliI CACNNNNGTG 1 cut(s) 617
PagI TCATGA 1 cut(s) 298
PaqCI CACCTGC 1 cut(s) 239
PdmI GAANNNNTTC 1 cut(s) 49
PfeI GAWTC 1 cut(s) 68
PkrI GCNGC 5 cut(s) 422, 455, 485, 566, 739
PleI GAGTC 1 cut(s) 342
PpsI GAGTC 1 cut(s) 342
Psp6I CCWGG 1 cut(s) 744
PspFI CCCAGC 1 cut(s) 719
PspGI CCWGG 1 cut(s) 744
PspN4I GGNNCC 1 cut(s) 613
PspPI GGNCC 1 cut(s) 220
PstI CTGCAG 1 cut(s) 739
RsaI GTAC 3 cut(s) 251, 269, 623
RsaNI GTAC 3 cut(s) 250, 268, 622
RseI CAYNNNNRTG 2 cut(s) 617, 651
SatI GCNGC 5 cut(s) 421, 454, 484, 565, 738
Sau3AI GATC 3 cut(s) 568, 675, 836
Sau96I GGNCC 1 cut(s) 220
SchI GAGTC 1 cut(s) 343
ScrFI CCNGG 1 cut(s) 746
SduI GDGCHC 1 cut(s) 614
SfaNI GCATC 7 cut(s) 159, 440, 462, 484, 560, 708, 758
SfcI CTRYAG 1 cut(s) 735
SinI GGWCC 1 cut(s) 220
SmiMI CAYNNNNRTG 2 cut(s) 617, 651
SmlI CTYRAG 1 cut(s) 753
SmoI CTYRAG 1 cut(s) 753
Sse9I AATT 1 cut(s) 101
SsiI CCGC 2 cut(s) 453, 656
SspMI CTAG 1 cut(s) 710
StyD4I CCNGG 1 cut(s) 744
StyI CCWWGG 2 cut(s) 96, 772
TaaI ACNGT 1 cut(s) 634
TaqI TCGA 2 cut(s) 404, 765
TasI AATT 1 cut(s) 101
TauI GCSGC 1 cut(s) 456
TfiI GAWTC 1 cut(s) 68
TseFI GTSAC 3 cut(s) 182, 638, 829
TseI GCWGC 4 cut(s) 420, 483, 564, 737
Tsp45I GTSAC 3 cut(s) 182, 638, 829
TspDTI ATGAA 2 cut(s) 287, 641
VpaK11BI GGWCC 1 cut(s) 220
XapI RAATTY 1 cut(s) 101
XceI RCATGY 1 cut(s) 727
XmnI GAANNNNTTC 1 cut(s) 49
XspI CTAG 1 cut(s) 710
Zsp2I ATGCAT 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.