MD06G1001400.v1.1

C1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0014254
Physical Location & Seq
Forward (+)
179148 .. 180507
1360 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1001400.v1.1.491

Sequence Viewer

Length: 870 bp
ATGATGATAAGCAATAAACACCCGAGGAAGACAACTTTTCTCCTCAAGAGGTCAGAATCAACTCCCCCCCAATCCCAACCCACAAATTTAGATAATATTGTCCCTAGAAATAACAAGTCTCCTTTGGTCGAATTCCCCACATCTCAGCTGATATTTGGGGAAGAGAGACTTCATTTCAGTCATCCACAACACCCCCTGTCCCAAGTGATCCTGCCCGACCTCTTCACCTGCGCAGGCTGCAAGGAGTACGGCGCCGGCAAGAGGTTCATTTGTCAACTATGTGACTTTCAGCTGCATGATTTTTGTGCCTTAGCTCCTCCAGCTCTAAAGAACCATCCCTTCCACATCCAACACCAACTTGTTTTCTATTCTAAAGCAGTTAAAGGAGGAATTGCACAATCCAAGTGCGATATTTGTCACAAGCCAAGCAAAGGTTATGCCTTCAGATGCAGCGCATGCAGCTTCCAGATGCATCCTTGCTGTGCGATGCTGTCATCAGAAATAAACCTCCAGACTCACCCACATACCCTAAGACTTTTATCAGCAGCTGATTCTTCTAGTAGGAGTTTTGTTTGTGGGGAGTGCAGGAGGAGGAGGTCGGGCAGGGTTTACCACTGCACGGTGTGCGATTACCATCTGCATGCAGTGTGCGCTAAGAACATGATAAATGGGTTGCAAGAGAACGGCCTCAAGGGACGTGAAAAGCCGAGTGTGTTGGGGACTGCTGCTCGCCTCGCGTCTCAAGTTGTCACTGAATTCATCGGCGGCCTCATTGAGGGGCTTGGGGAAGGTGTTGCTGATGTCTTGGTTCAAGATATTGCCAGGTCAGGAAGACCAAATGCCCGCCGATCATCCAATTCTCCTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2

Protein Analysis

290

Amino Acids

31.97

Weight (kDa)

9.09

Isoelectric Point (pI)

68.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C1_2 PF03107 59 - 103 9.7e-08 C1 domain
C1_2 PF03107 114 - 162 3.7e-06 C1 domain
C1_2 PF03107 172 - 218 3.2e-09 C1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20990
fragaria_vesca FvH4_5g26520
malus_domestica MD00G1031500.v1.1 MD06G1001400.v1.1
prunus_persica Prupe.5G002200_v2.0.a1
pyrus_communis pycom06g00150 pycom16g26300
rosa_chinensis RchiOBHm_Chr7g0217421
rosa_laevigata RLG00000002478
rosa_multiflora Rmu_sc0000145.1_g000014
rosa_roxburghii Rroxscaffold_3G00242090
rosa_rugosa Rorug07G0169000
rosa_samantha Rh7AG308700 Rh7BG299400 Rh7CG328100 Rh7DG308000
rosa_wichuraiana Rw7G026180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 236
Acc16I TGCGCA 1 cut(s) 232
Acc36I ACCTGC 1 cut(s) 236
AccB1I GGYRCC 1 cut(s) 251
AccII CGCG 1 cut(s) 737
AciI CCGC 2 cut(s) 765, 844
AclWI GGATC 1 cut(s) 202
AcsI RAATTY 3 cut(s) 85, 131, 755
AcuI CTGAAG 1 cut(s) 427
AcyI GRCGYC 1 cut(s) 252
AdeI CACNNNGTG 1 cut(s) 624
AfaI GTAC 1 cut(s) 248
AfiI CCNNNNNNNGG 4 cut(s) 261, 431, 619, 775
AgsI TTSAA 1 cut(s) 812
AjiI CACGTC 1 cut(s) 698
AjnI CCWGG 1 cut(s) 821
AluBI AGCT 6 cut(s) 148, 292, 314, 323, 462, 548
AluI AGCT 6 cut(s) 148, 292, 314, 323, 462, 548
Alw26I GTCTC 3 cut(s) 123, 160, 744
AlwI GGATC 1 cut(s) 202
AlwNI CAGNNNCTG 1 cut(s) 548
Ama87I CYCGRG 1 cut(s) 22
AoxI GGCC 2 cut(s) 685, 766
ApeKI GCWGC 6 cut(s) 237, 292, 450, 459, 545, 725
ApoI RAATTY 3 cut(s) 85, 131, 755
AspLEI GCGC 4 cut(s) 233, 254, 455, 653
AsuHPI GGTGA 2 cut(s) 217, 509
AvaI CYCGRG 1 cut(s) 22
BanI GGYRCC 1 cut(s) 251
BbsI GAAGAC 2 cut(s) 35, 838
BbvI GCAGC 6 cut(s) 224, 279, 462, 471, 557, 712
BccI CCATC 2 cut(s) 342, 642
BceAI ACGGC 2 cut(s) 265, 700
BciT130I CCWGG 1 cut(s) 823
BcoDI GTCTC 3 cut(s) 123, 160, 744
BfaI CTAG 2 cut(s) 105, 558
BfoI RGCGCY 1 cut(s) 255
BfuAI ACCTGC 1 cut(s) 236
BisI GCNGC 7 cut(s) 238, 293, 451, 460, 546, 726, 766
BlsI GCNGC 7 cut(s) 239, 294, 452, 461, 547, 727, 767
Bme1390I CCNGG 1 cut(s) 823
BmeT110I CYCGRG 1 cut(s) 22
BmgBI CACGTC 1 cut(s) 698
BmiI GGNNCC 1 cut(s) 253
BmrFI CCNGG 1 cut(s) 823
BmsI GCATC 4 cut(s) 437, 459, 477, 481
BpiI GAAGAC 2 cut(s) 35, 838
BpmI CTGGAG 2 cut(s) 303, 494
Bpu10I CCTNAGC 1 cut(s) 310
BpuEI CTTGAG 3 cut(s) 29, 674, 726
BsaBI GATNNNNATC 1 cut(s) 633
BsaHI GRCGYC 1 cut(s) 252
BsaJI CCNNGG 1 cut(s) 23
Bsc4I CCNNNNNNNGG 4 cut(s) 261, 431, 619, 775
Bse118I RCCGGY 1 cut(s) 254
Bse8I GATNNNNATC 1 cut(s) 633
BseBI CCWGG 1 cut(s) 823
BseDI CCNNGG 1 cut(s) 23
BseGI GGATG 5 cut(s) 181, 334, 345, 472, 851
BseJI GATNNNNATC 1 cut(s) 633
BseLI CCNNNNNNNGG 4 cut(s) 261, 431, 619, 775
BseMII CTCAG 1 cut(s) 158
BseRI GAGGAG 4 cut(s) 32, 306, 604, 607
BseXI GCAGC 6 cut(s) 224, 279, 462, 471, 557, 712
BsgI GTGCAG 2 cut(s) 601, 604
Bsh1236I CGCG 1 cut(s) 737
BshFI GGCC 2 cut(s) 687, 768
BshNI GGYRCC 1 cut(s) 251
BsiHKCI CYCGRG 1 cut(s) 22
BsiSI CCGG 1 cut(s) 255
BslFI GGGAC 4 cut(s) 86, 184, 708, 733
BslI CCNNNNNNNGG 4 cut(s) 261, 431, 619, 775
BsmAI GTCTC 3 cut(s) 123, 160, 744
BsmBI CGTCTC 1 cut(s) 744
BsmFI GGGAC 4 cut(s) 86, 184, 708, 733
BsnI GGCC 2 cut(s) 687, 768
BsoBI CYCGRG 1 cut(s) 22
Bsp143I GATC 2 cut(s) 207, 848
BspACI CCGC 2 cut(s) 765, 844
BspANI GGCC 2 cut(s) 687, 768
BspCNI CTCAG 1 cut(s) 157
BspFNI CGCG 1 cut(s) 737
BspLI GGNNCC 1 cut(s) 253
BspMI ACCTGC 1 cut(s) 236
BspPI GGATC 1 cut(s) 202
BspT107I GGYRCC 1 cut(s) 251
BsrFI RCCGGY 1 cut(s) 254
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 1 cut(s) 23
BssMI GATC 2 cut(s) 207, 848
BssNI GRCGYC 1 cut(s) 252
Bst2UI CCWGG 1 cut(s) 823
Bst4CI ACNGT 1 cut(s) 622
Bst6I CTCTTC 2 cut(s) 156, 227
BstACI GRCGYC 1 cut(s) 252
BstAPI GCANNNNNTGC 2 cut(s) 456, 624
BstC8I GCNNGC 6 cut(s) 235, 256, 457, 642, 730, 844
BstDEI CTNAG 4 cut(s) 144, 310, 530, 654
BstENI CCTNNNNNAGG 1 cut(s) 773
BstF5I GGATG 5 cut(s) 181, 334, 345, 472, 851
BstFNI CGCG 1 cut(s) 737
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 4 cut(s) 233, 254, 455, 653
BstKTI GATC 2 cut(s) 210, 851
BstMAI GTCTC 3 cut(s) 123, 160, 744
BstMBI GATC 2 cut(s) 207, 848
BstMWI GCNNNNNNNGC 7 cut(s) 237, 320, 456, 459, 624, 650, 734
BstNI CCWGG 1 cut(s) 823
BstNSI RCATGY 2 cut(s) 459, 644
BstSCI CCNGG 1 cut(s) 821
BstUI CGCG 1 cut(s) 737
BstV1I GCAGC 6 cut(s) 224, 279, 462, 471, 557, 712
BstV2I GAAGAC 2 cut(s) 35, 838
BsuRI GGCC 2 cut(s) 687, 768
BtgZI GCGATG 1 cut(s) 500
BtrI CACGTC 1 cut(s) 698
BtsCI GGATG 5 cut(s) 181, 334, 345, 472, 851
BtsI GCAGTG 2 cut(s) 613, 651
BtsIMutI CAGTG 3 cut(s) 613, 651, 750
BveI ACCTGC 1 cut(s) 236
Cac8I GCNNGC 6 cut(s) 235, 256, 457, 642, 730, 844
CaiI CAGNNNCTG 1 cut(s) 548
CfoI GCGC 4 cut(s) 233, 254, 455, 653
Cfr10I RCCGGY 1 cut(s) 254
CseI GACGC 1 cut(s) 726
Csp6I GTAC 1 cut(s) 247
CviAII CATG 4 cut(s) 296, 456, 641, 661
CviQI GTAC 1 cut(s) 247
DdeI CTNAG 4 cut(s) 144, 310, 530, 654
DinI GGCGCC 1 cut(s) 253
DpnI GATC 2 cut(s) 209, 850
DpnII GATC 2 cut(s) 207, 848
DraIII CACNNNGTG 1 cut(s) 624
Eam1104I CTCTTC 2 cut(s) 156, 227
EarI CTCTTC 2 cut(s) 156, 227
Eco57I CTGAAG 1 cut(s) 427
Eco88I CYCGRG 1 cut(s) 22
EcoNI CCTNNNNNAGG 1 cut(s) 773
EcoRI GAATTC 2 cut(s) 131, 755
EcoRII CCWGG 1 cut(s) 821
EcoT22I ATGCAT 1 cut(s) 474
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
Esp3I CGTCTC 1 cut(s) 744
FaeI CATG 4 cut(s) 299, 459, 644, 664
FaiI YATR 7 cut(s) 280, 297, 438, 457, 525, 642, 662
FalI AAGNNNNNCTT 2 cut(s) 153, 185
FaqI GGGAC 4 cut(s) 86, 184, 708, 733
FatI CATG 4 cut(s) 295, 455, 640, 660
FauI CCCGC 1 cut(s) 851
Fnu4HI GCNGC 7 cut(s) 238, 293, 451, 460, 546, 726, 766
FokI GGATG 5 cut(s) 168, 321, 332, 459, 838
Fsp4HI GCNGC 7 cut(s) 238, 293, 451, 460, 546, 726, 766
FspBI CTAG 2 cut(s) 105, 558
FspI TGCGCA 1 cut(s) 232
GlaI GCGC 4 cut(s) 232, 253, 454, 652
GluI GCNGC 7 cut(s) 238, 293, 451, 460, 546, 726, 766
GsuI CTGGAG 2 cut(s) 303, 494
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 2 cut(s) 687, 768
HapII CCGG 1 cut(s) 255
HgaI GACGC 1 cut(s) 726
HhaI GCGC 4 cut(s) 233, 254, 455, 653
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 4 cut(s) 299, 459, 644, 664
Hin6I GCGC 4 cut(s) 231, 252, 453, 651
HinP1I GCGC 4 cut(s) 231, 252, 453, 651
HincII GTYRAC 1 cut(s) 275
HindII GTYRAC 1 cut(s) 275
HinfI GANTC 3 cut(s) 56, 514, 551
HpaII CCGG 1 cut(s) 255
HphI GGTGA 2 cut(s) 217, 509
Hpy166II GTNNAC 2 cut(s) 275, 610
Hpy188I TCNGA 3 cut(s) 55, 446, 499
Hpy188III TCNNGA 6 cut(s) 46, 466, 511, 812, 828, 863
Hpy8I GTNNAC 2 cut(s) 275, 610
HpyAV CCTTC 3 cut(s) 349, 451, 782
HpyCH4III ACNGT 1 cut(s) 622
HpyCH4IV ACGT 1 cut(s) 697
HpyF10VI GCNNNNNNNGC 7 cut(s) 237, 320, 456, 459, 624, 650, 734
HpyF3I CTNAG 4 cut(s) 144, 310, 530, 654
HpySE526I ACGT 1 cut(s) 697
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 4 cut(s) 299, 459, 644, 664
HspAI GCGC 4 cut(s) 231, 252, 453, 651
KasI GGCGCC 1 cut(s) 251
KroI GCCGGC 1 cut(s) 254
KroNI GCCGGC 1 cut(s) 256
Kzo9I GATC 2 cut(s) 207, 848
LmnI GCTCC 1 cut(s) 319
Lsp1109I GCAGC 6 cut(s) 224, 279, 462, 471, 557, 712
LweI GCATC 4 cut(s) 437, 459, 477, 481
MaeI CTAG 2 cut(s) 105, 558
MaeII ACGT 1 cut(s) 697
MaeIII GTNAC 3 cut(s) 281, 416, 748
MalI GATC 2 cut(s) 209, 850
MboI GATC 2 cut(s) 207, 848
MboII GAAGA 5 cut(s) 40, 173, 214, 546, 843
MluCI AATT 5 cut(s) 85, 131, 390, 755, 856
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 1 cut(s) 508
MmeI TCCRAC 1 cut(s) 373
Mph1103I ATGCAT 1 cut(s) 474
MroNI GCCGGC 1 cut(s) 254
MseI TTAA 1 cut(s) 381
MslI CAYNNNNRTG 1 cut(s) 639
MspA1I CMGCKG 3 cut(s) 148, 292, 548
MspI CCGG 1 cut(s) 255
MspR9I CCNGG 1 cut(s) 823
MvaI CCWGG 1 cut(s) 823
MvnI CGCG 1 cut(s) 737
MwoI GCNNNNNNNGC 7 cut(s) 237, 320, 456, 459, 624, 650, 734
NaeI GCCGGC 1 cut(s) 256
NarI GGCGCC 1 cut(s) 252
NdeII GATC 2 cut(s) 207, 848
NgoMIV GCCGGC 1 cut(s) 254
NlaIII CATG 4 cut(s) 299, 459, 644, 664
NlaIV GGNNCC 1 cut(s) 253
NmeAIII GCCGAG 1 cut(s) 732
NmuCI GTSAC 3 cut(s) 281, 416, 748
NsbI TGCGCA 1 cut(s) 232
NsiI ATGCAT 1 cut(s) 474
NspI RCATGY 2 cut(s) 459, 644
PaeI GCATGC 2 cut(s) 459, 644
PaqCI CACCTGC 1 cut(s) 236
PdiI GCCGGC 1 cut(s) 256
PfeI GAWTC 2 cut(s) 56, 551
PkrI GCNGC 7 cut(s) 239, 294, 452, 461, 547, 727, 767
PleI GAGTC 1 cut(s) 508
PluTI GGCGCC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 508
Psp6I CCWGG 1 cut(s) 821
PspGI CCWGG 1 cut(s) 821
PspN4I GGNNCC 1 cut(s) 253
PstNI CAGNNNCTG 1 cut(s) 548
PvuII CAGCTG 3 cut(s) 148, 292, 548
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
RseI CAYNNNNRTG 1 cut(s) 639
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 7 cut(s) 238, 293, 451, 460, 546, 726, 766
Sau3AI GATC 2 cut(s) 207, 848
SchI GAGTC 1 cut(s) 508
ScrFI CCNGG 1 cut(s) 823
SfaNI GCATC 4 cut(s) 437, 459, 477, 481
SfoI GGCGCC 1 cut(s) 253
SmiMI CAYNNNNRTG 1 cut(s) 639
SmlI CTYRAG 3 cut(s) 44, 689, 741
SmoI CTYRAG 3 cut(s) 44, 689, 741
SphI GCATGC 2 cut(s) 459, 644
Sse9I AATT 5 cut(s) 85, 131, 390, 755, 856
SsiI CCGC 2 cut(s) 765, 844
SspDI GGCGCC 1 cut(s) 251
SspI AATATT 1 cut(s) 97
SspMI CTAG 2 cut(s) 105, 558
StyD4I CCNGG 1 cut(s) 821
TaaI ACNGT 1 cut(s) 622
TaiI ACGT 1 cut(s) 700
TaqI TCGA 1 cut(s) 129
TasI AATT 5 cut(s) 85, 131, 390, 755, 856
TauI GCSGC 1 cut(s) 768
TfiI GAWTC 2 cut(s) 56, 551
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TscAI CASTG 3 cut(s) 620, 651, 757
TseFI GTSAC 3 cut(s) 281, 416, 748
TseI GCWGC 6 cut(s) 237, 292, 450, 459, 545, 725
Tsp45I GTSAC 3 cut(s) 281, 416, 748
TspDTI ATGAA 3 cut(s) 161, 256, 748
TspRI CASTG 3 cut(s) 620, 651, 757
XagI CCTNNNNNAGG 1 cut(s) 773
XapI RAATTY 3 cut(s) 85, 131, 755
XceI RCATGY 2 cut(s) 459, 644
XspI CTAG 2 cut(s) 105, 558
Zsp2I ATGCAT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.