MD00G1037300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0011233
Physical Location & Seq
Forward (+)
6599595 .. 6603719
4125 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1037300.v1.1.491

Sequence Viewer

Length: 900 bp
ATGTCATTCACTTGCCTCCAGCTATTAGTAAATTCACCTCCGCGACCTCGACTCGCCCTCTACATCGCCGCCGCATCTTTCCCCAAAACCCCAGCCATTCTCTCTCTCCCAATCATCAGTCGCAGGCATTTCCCAATCTCAAACAACAGTCCTGTAAATCCCCAAAATTTCAGCTCCCATCAATCTCGTCTTGGAGTGTACAACTCCGATGCAACTCTGCAACCAAATGACGCCGTCAGTGGTGCTTTCAATTTGGACTATTTCTTGTCGATTGCGGAGTTTTTGTGCATTACGTCGTCGGCGGTTGTTTCTATTGGTTTCGCGGTCAATTGCGCGGTTTTGAGCTTAAAGAAGACGTCTTTAGTGGCAATGGGGAACTGGGTTTTGGCGTCTGGGGCTGTGGCGTTGGTAATGGCCGTAGGGATCGGGGCGTGGATTCGGCGGCGGCAGTGGAGGCGAATGTGCAGGGAGTCGGTGAAGGGTGGGATGGAGTTGAATTTGTTTGAGAGAATTGAGAAATTGGAAGAGGATTTGCGGAGCTCGACTACAATCATTCGGGTTTTGTCAAGGCAGCTCGAGAAATTGGGGATAAGATTTCGGGTTACCCGTAAGGCTTTGAAAGAGCCCATTGCTGAGACTGCAGCTTTGGCTCAAAAGAATTCTGAGGCCACTCGAGCATTAGCAGCACAAGAAGACATTTTGGAAAAGGAGCTTGGAGAAATTCAAAAAGTTTTGCTGGCAATGCAGCAGCAGGAAAAACAACTTGAGCTAATTCTTGCAATTGCGACTTCTGGGAAGCTACGGGAAAGTAGACAGGTACGCGACCAGGAACAAAGTACCACTATAGTACCTAATTCAAGTGAGGAGGATTTAAAACAGAAGGAAGCCCACCAAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

300

Amino Acids

33.17

Weight (kDa)

9.45

Isoelectric Point (pI)

53.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 359
AccI GTMKAC 1 cut(s) 811
AccII CGCG 4 cut(s) 43, 323, 335, 822
AclWI GGATC 1 cut(s) 431
AcoI YGGCCR 1 cut(s) 414
AcsI RAATTY 5 cut(s) 31, 166, 496, 658, 720
AcyI GRCGYC 3 cut(s) 231, 356, 389
AfaI GTAC 4 cut(s) 200, 819, 838, 849
AgsI TTSAA 5 cut(s) 250, 496, 619, 725, 858
AjnI CCWGG 1 cut(s) 825
AjuI GAANNNNNNNTTGG 4 cut(s) 368, 400, 696, 728
AluBI AGCT 9 cut(s) 22, 174, 345, 540, 574, 644, 712, 769, 799
AluI AGCT 9 cut(s) 22, 174, 345, 540, 574, 644, 712, 769, 799
Alw21I GWGCWC 1 cut(s) 542
Alw26I GTCTC 1 cut(s) 629
AlwI GGATC 1 cut(s) 431
Ama87I CYCGRG 2 cut(s) 575, 672
AoxI GGCC 2 cut(s) 414, 666
ApeKI GCWGC 5 cut(s) 571, 641, 683, 745, 748
ApoI RAATTY 5 cut(s) 31, 166, 496, 658, 720
ArsI GACNNNNNNTTYG 2 cut(s) 628, 660
AspLEI GCGC 1 cut(s) 335
AsuHPI GGTGA 2 cut(s) 27, 487
AvaI CYCGRG 2 cut(s) 575, 672
BaeI ACNNNNGTAYC 2 cut(s) 831, 864
BanII GRGCYC 2 cut(s) 542, 627
BbsI GAAGAC 2 cut(s) 359, 699
Bbv12I GWGCWC 1 cut(s) 542
BbvI GCAGC 5 cut(s) 583, 653, 695, 757, 760
BccI CCATC 2 cut(s) 186, 481
BceAI ACGGC 2 cut(s) 218, 401
BciT130I CCWGG 1 cut(s) 827
BcoDI GTCTC 1 cut(s) 629
BfmI CTRYAG 2 cut(s) 639, 843
BisI GCNGC 9 cut(s) 69, 72, 443, 446, 572, 642, 684, 746, 749
BlsI GCNGC 9 cut(s) 70, 73, 444, 447, 573, 643, 685, 747, 750
Bme1390I CCNGG 1 cut(s) 827
BmeT110I CYCGRG 2 cut(s) 575, 672
BmrFI CCNGG 1 cut(s) 827
BmrI ACTGGG 1 cut(s) 388
BmsI GCATC 2 cut(s) 83, 199
BmuI ACTGGG 1 cut(s) 388
BpiI GAAGAC 2 cut(s) 359, 699
BpuEI CTTGAG 1 cut(s) 785
BsaHI GRCGYC 3 cut(s) 231, 356, 389
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bse1I ACTGG 1 cut(s) 383
Bse3DI GCAATG 3 cut(s) 375, 627, 747
BseBI CCWGG 1 cut(s) 827
BseGI GGATG 1 cut(s) 492
BseMI GCAATG 3 cut(s) 375, 627, 747
BseMII CTCAG 2 cut(s) 624, 654
BseNI ACTGG 1 cut(s) 383
BseRI GAGGAG 1 cut(s) 878
BseXI GCAGC 5 cut(s) 583, 653, 695, 757, 760
BseYI CCCAGC 1 cut(s) 91
BsgI GTGCAG 1 cut(s) 484
Bsh1236I CGCG 4 cut(s) 43, 323, 335, 822
BshFI GGCC 2 cut(s) 416, 668
BsiHKAI GWGCWC 1 cut(s) 542
BsiHKCI CYCGRG 2 cut(s) 575, 672
BsmAI GTCTC 1 cut(s) 629
BsnI GGCC 2 cut(s) 416, 668
BsoBI CYCGRG 2 cut(s) 575, 672
Bsp1286I GDGCHC 2 cut(s) 542, 627
Bsp1407I TGTACA 1 cut(s) 198
Bsp143I GATC 1 cut(s) 423
BspANI GGCC 2 cut(s) 416, 668
BspCNI CTCAG 2 cut(s) 625, 655
BspFNI CGCG 4 cut(s) 43, 323, 335, 822
BspMAI CTGCAG 1 cut(s) 643
BspPI GGATC 1 cut(s) 431
BsrDI GCAATG 3 cut(s) 375, 627, 747
BsrGI TGTACA 1 cut(s) 198
BsrI ACTGG 1 cut(s) 383
BssMI GATC 1 cut(s) 423
BssNI GRCGYC 3 cut(s) 231, 356, 389
Bst2UI CCWGG 1 cut(s) 827
Bst4CI ACNGT 1 cut(s) 149
Bst6I CTCTTC 1 cut(s) 519
BstACI GRCGYC 3 cut(s) 231, 356, 389
BstAUI TGTACA 1 cut(s) 198
BstC8I GCNNGC 2 cut(s) 125, 738
BstDEI CTNAG 2 cut(s) 633, 663
BstEII GGTNACC 1 cut(s) 601
BstF5I GGATG 1 cut(s) 492
BstFNI CGCG 4 cut(s) 43, 323, 335, 822
BstHHI GCGC 1 cut(s) 335
BstKTI GATC 1 cut(s) 426
BstMAI GTCTC 1 cut(s) 629
BstMBI GATC 1 cut(s) 423
BstMWI GCNNNNNNNGC 7 cut(s) 395, 454, 638, 647, 674, 683, 742
BstNI CCWGG 1 cut(s) 827
BstPI GGTNACC 1 cut(s) 601
BstSCI CCNGG 1 cut(s) 825
BstSFI CTRYAG 2 cut(s) 639, 843
BstUI CGCG 4 cut(s) 43, 323, 335, 822
BstV1I GCAGC 5 cut(s) 583, 653, 695, 757, 760
BstV2I GAAGAC 2 cut(s) 359, 699
BsuRI GGCC 2 cut(s) 416, 668
BtgZI GCGATG 1 cut(s) 49
BtsCI GGATG 1 cut(s) 492
BtsI GCAGTG 1 cut(s) 455
BtsIMutI CAGTG 2 cut(s) 244, 455
Cac8I GCNNGC 2 cut(s) 125, 738
CfoI GCGC 1 cut(s) 335
CseI GACGC 2 cut(s) 239, 378
Csp6I GTAC 4 cut(s) 199, 818, 837, 848
CviQI GTAC 4 cut(s) 199, 818, 837, 848
DdeI CTNAG 2 cut(s) 633, 663
DpnI GATC 1 cut(s) 425
DpnII GATC 1 cut(s) 423
DraI TTTAAA 1 cut(s) 873
EaeI YGGCCR 1 cut(s) 414
Eam1104I CTCTTC 1 cut(s) 519
EarI CTCTTC 1 cut(s) 519
Ecl136II GAGCTC 1 cut(s) 540
Eco24I GRGCYC 2 cut(s) 542, 627
Eco53kI GAGCTC 1 cut(s) 540
Eco88I CYCGRG 2 cut(s) 575, 672
Eco91I GGTNACC 1 cut(s) 601
EcoICRI GAGCTC 1 cut(s) 540
EcoO65I GGTNACC 1 cut(s) 601
EcoRI GAATTC 1 cut(s) 658
EcoRII CCWGG 1 cut(s) 825
EcoT38I GRGCYC 2 cut(s) 542, 627
FaiI YATR 2 cut(s) 845, 898
FblI GTMKAC 1 cut(s) 811
Fnu4HI GCNGC 9 cut(s) 69, 72, 443, 446, 572, 642, 684, 746, 749
FokI GGATG 1 cut(s) 499
FriOI GRGCYC 2 cut(s) 542, 627
Fsp4HI GCNGC 9 cut(s) 69, 72, 443, 446, 572, 642, 684, 746, 749
GlaI GCGC 1 cut(s) 334
GluI GCNGC 9 cut(s) 69, 72, 443, 446, 572, 642, 684, 746, 749
GsaI CCCAGC 1 cut(s) 95
HaeIII GGCC 2 cut(s) 416, 668
HgaI GACGC 2 cut(s) 239, 378
HhaI GCGC 1 cut(s) 335
Hin1I GRCGYC 3 cut(s) 231, 356, 389
Hin6I GCGC 1 cut(s) 333
HinP1I GCGC 1 cut(s) 333
HinfI GANTC 3 cut(s) 51, 436, 470
HphI GGTGA 2 cut(s) 27, 487
Hpy166II GTNNAC 2 cut(s) 199, 812
Hpy188I TCNGA 2 cut(s) 208, 664
Hpy188III TCNNGA 1 cut(s) 577
Hpy8I GTNNAC 2 cut(s) 199, 812
Hpy99I CGWCG 2 cut(s) 298, 301
HpyAV CCTTC 2 cut(s) 472, 874
HpyCH4III ACNGT 1 cut(s) 149
HpyCH4IV ACGT 2 cut(s) 293, 356
HpyCH4V TGCA 7 cut(s) 212, 220, 288, 465, 641, 745, 779
HpyF10VI GCNNNNNNNGC 7 cut(s) 395, 454, 638, 647, 674, 683, 742
HpyF3I CTNAG 2 cut(s) 633, 663
HpySE526I ACGT 2 cut(s) 293, 356
Hsp92I GRCGYC 3 cut(s) 231, 356, 389
HspAI GCGC 1 cut(s) 333
Kzo9I GATC 1 cut(s) 423
LmnI GCTCC 3 cut(s) 179, 537, 709
Lsp1109I GCAGC 5 cut(s) 583, 653, 695, 757, 760
LweI GCATC 2 cut(s) 83, 199
MaeII ACGT 2 cut(s) 293, 356
MaeIII GTNAC 1 cut(s) 601
MalI GATC 1 cut(s) 425
MboI GATC 1 cut(s) 423
MboII GAAGA 3 cut(s) 364, 536, 704
MfeI CAATTG 2 cut(s) 328, 780
MhlI GDGCHC 2 cut(s) 542, 627
MlyI GAGTC 2 cut(s) 45, 479
MnlI CCTC 9 cut(s) 26, 48, 57, 68, 447, 520, 658, 856, 859
MseI TTAA 2 cut(s) 347, 872
MspR9I CCNGG 1 cut(s) 827
MunI CAATTG 2 cut(s) 328, 780
MvaI CCWGG 1 cut(s) 827
MvnI CGCG 4 cut(s) 43, 323, 335, 822
MwoI GCNNNNNNNGC 7 cut(s) 395, 454, 638, 647, 674, 683, 742
NdeII GATC 1 cut(s) 423
PaeR7I CTCGAG 2 cut(s) 575, 672
PcsI WCGNNNNNNNCGW 1 cut(s) 604
PfeI GAWTC 1 cut(s) 436
PflFI GACNNNGTC 1 cut(s) 233
PkrI GCNGC 9 cut(s) 70, 73, 444, 447, 573, 643, 685, 747, 750
PleI GAGTC 2 cut(s) 45, 478
PpsI GAGTC 2 cut(s) 45, 478
Psp124BI GAGCTC 1 cut(s) 542
Psp6I CCWGG 1 cut(s) 825
PspEI GGTNACC 1 cut(s) 601
PspFI CCCAGC 1 cut(s) 91
PspGI CCWGG 1 cut(s) 825
PspXI VCTCGAGB 1 cut(s) 672
PstI CTGCAG 1 cut(s) 643
PsyI GACNNNGTC 1 cut(s) 233
RsaI GTAC 4 cut(s) 200, 819, 838, 849
RsaNI GTAC 4 cut(s) 199, 818, 837, 848
SacI GAGCTC 1 cut(s) 542
SaqAI TTAA 2 cut(s) 347, 872
SatI GCNGC 9 cut(s) 69, 72, 443, 446, 572, 642, 684, 746, 749
Sau3AI GATC 1 cut(s) 423
SchI GAGTC 2 cut(s) 45, 479
ScrFI CCNGG 1 cut(s) 827
SduI GDGCHC 2 cut(s) 542, 627
SfaNI GCATC 2 cut(s) 83, 199
SfcI CTRYAG 2 cut(s) 639, 843
Sfr274I CTCGAG 2 cut(s) 575, 672
SlaI CTCGAG 2 cut(s) 575, 672
SmlI CTYRAG 3 cut(s) 575, 672, 764
SmoI CTYRAG 3 cut(s) 575, 672, 764
SstI GAGCTC 1 cut(s) 542
StyD4I CCNGG 1 cut(s) 825
TaaI ACNGT 1 cut(s) 149
TaiI ACGT 2 cut(s) 296, 359
TaqI TCGA 5 cut(s) 49, 269, 542, 576, 673
TatI WGTACW 1 cut(s) 198
TauI GCSGC 4 cut(s) 71, 74, 445, 448
TfiI GAWTC 1 cut(s) 436
Tru1I TTAA 2 cut(s) 347, 872
Tru9I TTAA 2 cut(s) 347, 872
TscAI CASTG 2 cut(s) 244, 455
TseI GCWGC 5 cut(s) 571, 641, 683, 745, 748
TspRI CASTG 2 cut(s) 244, 455
Tth111I GACNNNGTC 1 cut(s) 233
XapI RAATTY 5 cut(s) 31, 166, 496, 658, 720
XhoI CTCGAG 2 cut(s) 575, 672
XmiI GTMKAC 1 cut(s) 811
ZraI GACGTC 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.