MD01G1033600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0011233
Physical Location & Seq
Reverse (-)
11599903 .. 11603125
3223 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1033600.v1.1.491

Sequence Viewer

Length: 411 bp
ATGGGTTTCGCTATGAATTGCGCGGCTATGAGCTTAAATAAGACGTCTTTTGTGGCAATGGGAAACTGGGGTCTAGCGTCTTGGGCCCTGGCGTTGGTAATGGTTGTTGGGATTGGGGCGTGGATTCAGCGGCTGCAGTGGAGGTGGATGTGTAGGGAGTCGGTGAAGGGCGGGATGGAGGTGAATTTGTTTGAGAGAATTGAGAAATTGGAGGAGGATTTGAGGAGCTCAGCCACAATCATGTGTGTCTTGTCAAGGCAGCTTGAGAAATTGGGGATACGGTTTCGGGTTACCCCTTTGGCTCAAAAGAATTATGAGGCCACTCGAGTATTAGCAGCACAAGAAGACATTTTGGAAAAAGAGCTTGGAGAAATTCAAAAGGTTTTGCTGGCTATGCAGGATGAGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

15.44

Weight (kDa)

6.33

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 47
AccII CGCG 1 cut(s) 23
AciI CCGC 3 cut(s) 23, 130, 171
AcsI RAATTY 2 cut(s) 184, 372
AcyI GRCGYC 1 cut(s) 44
AfiI CCNNNNNNNGG 1 cut(s) 94
AgsI TTSAA 1 cut(s) 377
AjnI CCWGG 1 cut(s) 87
AjuI GAANNNNNNNTTGG 2 cut(s) 348, 380
AluBI AGCT 4 cut(s) 33, 228, 262, 364
AluI AGCT 4 cut(s) 33, 228, 262, 364
Alw21I GWGCWC 1 cut(s) 230
AlwNI CAGNNNCTG 1 cut(s) 133
Ama87I CYCGRG 1 cut(s) 324
AoxI GGCC 2 cut(s) 84, 318
ApaI GGGCCC 1 cut(s) 88
ApeKI GCWGC 3 cut(s) 133, 259, 335
ApoI RAATTY 2 cut(s) 184, 372
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 2 cut(s) 84, 85
AsuHPI GGTGA 2 cut(s) 175, 193
AvaI CYCGRG 1 cut(s) 324
BaeGI GKGCMC 1 cut(s) 88
BanII GRGCYC 2 cut(s) 88, 230
BbsI GAAGAC 1 cut(s) 351
Bbv12I GWGCWC 1 cut(s) 230
BbvI GCAGC 3 cut(s) 120, 271, 347
BccI CCATC 1 cut(s) 169
BciT130I CCWGG 1 cut(s) 89
BciVI GTATCC 1 cut(s) 270
BfaI CTAG 1 cut(s) 74
BfmI CTRYAG 1 cut(s) 134
BfuI GTATCC 1 cut(s) 270
BisI GCNGC 5 cut(s) 24, 131, 134, 260, 336
BlpI GCTNAGC 1 cut(s) 229
BlsI GCNGC 5 cut(s) 25, 132, 135, 261, 337
Bme1390I CCNGG 1 cut(s) 89
BmeT110I CYCGRG 1 cut(s) 324
BmgT120I GGNCC 2 cut(s) 84, 85
BmiI GGNNCC 1 cut(s) 86
BmrFI CCNGG 1 cut(s) 89
BmrI ACTGGG 1 cut(s) 76
BmuI ACTGGG 1 cut(s) 76
BpiI GAAGAC 1 cut(s) 351
Bpu1102I GCTNAGC 1 cut(s) 229
BpuEI CTTGAG 1 cut(s) 284
BsaHI GRCGYC 1 cut(s) 44
BsaJI CCNNGG 1 cut(s) 87
Bsc4I CCNNNNNNNGG 1 cut(s) 94
Bse1I ACTGG 1 cut(s) 71
Bse3DI GCAATG 1 cut(s) 63
BseBI CCWGG 1 cut(s) 89
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 3 cut(s) 153, 180, 406
BseLI CCNNNNNNNGG 1 cut(s) 94
BseMI GCAATG 1 cut(s) 63
BseMII CTCAG 1 cut(s) 243
BseNI ACTGG 1 cut(s) 71
BseRI GAGGAG 2 cut(s) 227, 238
BseSI GKGCMC 1 cut(s) 88
BseXI GCAGC 3 cut(s) 120, 271, 347
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 2 cut(s) 86, 320
BsiHKAI GWGCWC 1 cut(s) 230
BsiHKCI CYCGRG 1 cut(s) 324
BslI CCNNNNNNNGG 1 cut(s) 94
BsnI GGCC 2 cut(s) 86, 320
BsoBI CYCGRG 1 cut(s) 324
Bsp120I GGGCCC 1 cut(s) 84
Bsp1286I GDGCHC 2 cut(s) 88, 230
Bsp1720I GCTNAGC 1 cut(s) 229
BspACI CCGC 3 cut(s) 23, 130, 171
BspANI GGCC 2 cut(s) 86, 320
BspCNI CTCAG 1 cut(s) 242
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 1 cut(s) 86
BspMAI CTGCAG 1 cut(s) 138
BsrDI GCAATG 1 cut(s) 63
BsrI ACTGG 1 cut(s) 71
BssECI CCNNGG 1 cut(s) 87
BssNI GRCGYC 1 cut(s) 44
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 1 cut(s) 282
BstACI GRCGYC 1 cut(s) 44
BstC8I GCNNGC 1 cut(s) 390
BstDEI CTNAG 1 cut(s) 229
BstEII GGTNACC 1 cut(s) 289
BstF5I GGATG 3 cut(s) 153, 180, 406
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 1 cut(s) 23
BstMWI GCNNNNNNNGC 3 cut(s) 83, 394, 403
BstNI CCWGG 1 cut(s) 89
BstPI GGTNACC 1 cut(s) 289
BstSCI CCNGG 1 cut(s) 87
BstSFI CTRYAG 1 cut(s) 134
BstSLI GKGCMC 1 cut(s) 88
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 3 cut(s) 120, 271, 347
BstV2I GAAGAC 1 cut(s) 351
BsuI GTATCC 1 cut(s) 270
BsuRI GGCC 2 cut(s) 86, 320
BtsCI GGATG 3 cut(s) 153, 180, 406
BtsI GCAGTG 1 cut(s) 143
BtsIMutI CAGTG 1 cut(s) 143
Cac8I GCNNGC 1 cut(s) 390
CaiI CAGNNNCTG 1 cut(s) 133
CfoI GCGC 1 cut(s) 23
Cfr13I GGNCC 2 cut(s) 84, 85
CseI GACGC 1 cut(s) 66
CviAII CATG 1 cut(s) 241
DdeI CTNAG 1 cut(s) 229
Ecl136II GAGCTC 1 cut(s) 228
Eco24I GRGCYC 2 cut(s) 88, 230
Eco53kI GAGCTC 1 cut(s) 228
Eco88I CYCGRG 1 cut(s) 324
Eco91I GGTNACC 1 cut(s) 289
EcoICRI GAGCTC 1 cut(s) 228
EcoO109I RGGNCCY 1 cut(s) 85
EcoO65I GGTNACC 1 cut(s) 289
EcoRII CCWGG 1 cut(s) 87
EcoT38I GRGCYC 2 cut(s) 88, 230
FaeI CATG 1 cut(s) 244
FaiI YATR 5 cut(s) 14, 29, 242, 315, 395
FatI CATG 1 cut(s) 240
FauI CCCGC 1 cut(s) 164
Fnu4HI GCNGC 5 cut(s) 24, 131, 134, 260, 336
FokI GGATG 2 cut(s) 160, 187
FriOI GRGCYC 2 cut(s) 88, 230
Fsp4HI GCNGC 5 cut(s) 24, 131, 134, 260, 336
FspBI CTAG 1 cut(s) 74
GlaI GCGC 1 cut(s) 22
GluI GCNGC 5 cut(s) 24, 131, 134, 260, 336
HaeIII GGCC 2 cut(s) 86, 320
HgaI GACGC 1 cut(s) 66
HhaI GCGC 1 cut(s) 23
Hin1I GRCGYC 1 cut(s) 44
Hin1II CATG 1 cut(s) 244
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HinfI GANTC 2 cut(s) 124, 158
HphI GGTGA 2 cut(s) 175, 193
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 1 cut(s) 282
HpyCH4IV ACGT 1 cut(s) 44
HpyCH4V TGCA 2 cut(s) 136, 397
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 394, 403
HpyF3I CTNAG 1 cut(s) 229
HpySE526I ACGT 1 cut(s) 44
Hsp92I GRCGYC 1 cut(s) 44
Hsp92II CATG 1 cut(s) 244
HspAI GCGC 1 cut(s) 21
LmnI GCTCC 1 cut(s) 225
LpnPI CCDG 5 cut(s) 52, 74, 101, 374, 383
Lsp1109I GCAGC 3 cut(s) 120, 271, 347
MaeI CTAG 1 cut(s) 74
MaeII ACGT 1 cut(s) 44
MaeIII GTNAC 1 cut(s) 289
MboII GAAGA 1 cut(s) 356
MhlI GDGCHC 2 cut(s) 88, 230
MluCI AATT 7 cut(s) 16, 184, 198, 206, 269, 310, 372
MlyI GAGTC 1 cut(s) 167
MnlI CCTC 6 cut(s) 135, 172, 205, 208, 216, 310
MseI TTAA 1 cut(s) 35
MslI CAYNNNNRTG 1 cut(s) 239
MspA1I CMGCKG 1 cut(s) 130
MspR9I CCNGG 1 cut(s) 89
MvaI CCWGG 1 cut(s) 89
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 3 cut(s) 83, 394, 403
NlaIII CATG 1 cut(s) 244
NlaIV GGNNCC 1 cut(s) 86
PaeR7I CTCGAG 1 cut(s) 324
PfeI GAWTC 1 cut(s) 124
PkrI GCNGC 5 cut(s) 25, 132, 135, 261, 337
PleI GAGTC 1 cut(s) 166
PpsI GAGTC 1 cut(s) 166
Psp124BI GAGCTC 1 cut(s) 230
Psp6I CCWGG 1 cut(s) 87
PspEI GGTNACC 1 cut(s) 289
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 1 cut(s) 86
PspOMI GGGCCC 1 cut(s) 84
PspPI GGNCC 2 cut(s) 84, 85
PspXI VCTCGAGB 1 cut(s) 324
PstI CTGCAG 1 cut(s) 138
PstNI CAGNNNCTG 1 cut(s) 133
RseI CAYNNNNRTG 1 cut(s) 239
SacI GAGCTC 1 cut(s) 230
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 5 cut(s) 24, 131, 134, 260, 336
Sau96I GGNCC 2 cut(s) 84, 85
SchI GAGTC 1 cut(s) 167
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 2 cut(s) 88, 230
SetI ASST 8 cut(s) 35, 47, 146, 183, 230, 264, 366, 384
SfcI CTRYAG 1 cut(s) 134
Sfr274I CTCGAG 1 cut(s) 324
SlaI CTCGAG 1 cut(s) 324
SmiMI CAYNNNNRTG 1 cut(s) 239
SmlI CTYRAG 2 cut(s) 263, 324
SmoI CTYRAG 2 cut(s) 263, 324
Sse9I AATT 7 cut(s) 16, 184, 198, 206, 269, 310, 372
SsiI CCGC 3 cut(s) 23, 130, 171
SspMI CTAG 1 cut(s) 74
SstI GAGCTC 1 cut(s) 230
StyD4I CCNGG 1 cut(s) 87
TaaI ACNGT 1 cut(s) 282
TaiI ACGT 1 cut(s) 47
TaqI TCGA 1 cut(s) 325
TasI AATT 7 cut(s) 16, 184, 198, 206, 269, 310, 372
TauI GCSGC 2 cut(s) 26, 133
TfiI GAWTC 1 cut(s) 124
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TscAI CASTG 1 cut(s) 143
TseI GCWGC 3 cut(s) 133, 259, 335
TspDTI ATGAA 1 cut(s) 29
TspRI CASTG 1 cut(s) 143
XapI RAATTY 2 cut(s) 184, 372
XhoI CTCGAG 1 cut(s) 324
XspI CTAG 1 cut(s) 74
ZraI GACGTC 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.