MD00G1092800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0006897
Physical Location & Seq
Forward (+)
19553464 .. 19554730
1267 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1092800.v1.1.491

Sequence Viewer

Length: 327 bp
ATGGGCACCAAAGAGCTTCCGGACTCCGCCTCTCAGCGCCGGAACTGGCAGAACATCTTCAACGCGCTGGCACAGATGCTGCGGTCTCAGCAATCGAACCTCGAGACGATGGCCGGCGACAGAAAAGTCTTCAAAGATCACGTCAGAGTGCAAGAAGAGAAGTGGATCTCCGCGTTTCATCTCTTGGGAGATCAAATCCGCCAGATGAAGGTGGACTTGGAATTGAAAGAAATGGAAGGCTCCGTTGAGGCGGCCAAGTTAGGGTGGGCTCTTAGTATGGAGCAACAAGAGGCTTATATCGCGAAACTCAAAATACTCAGACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

109

Amino Acids

12.55

Weight (kDa)

8.03

Isoelectric Point (pI)

46.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 125
AccB1I GGYRCC 1 cut(s) 5
AccII CGCG 3 cut(s) 65, 173, 302
AccIII TCCGGA 1 cut(s) 19
AciI CCGC 5 cut(s) 27, 82, 171, 199, 251
AclWI GGATC 1 cut(s) 173
AcoI YGGCCR 2 cut(s) 111, 252
AfiI CCNNNNNNNGG 2 cut(s) 208, 261
AgsI TTSAA 3 cut(s) 61, 133, 226
AjiI CACGTC 1 cut(s) 142
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 1 cut(s) 16
AluI AGCT 1 cut(s) 16
Alw26I GTCTC 2 cut(s) 90, 98
AlwI GGATC 1 cut(s) 173
AlwNI CAGNNNCTG 1 cut(s) 79
Ama87I CYCGRG 1 cut(s) 101
Aor13HI TCCGGA 1 cut(s) 19
AoxI GGCC 2 cut(s) 111, 252
ApeKI GCWGC 1 cut(s) 79
ArsI GACNNNNNNTTYG 2 cut(s) 126, 158
Asp700I GAANNNNTTC 1 cut(s) 56
AspLEI GCGC 2 cut(s) 39, 67
AvaI CYCGRG 1 cut(s) 101
BaeGI GKGCMC 1 cut(s) 8
BanI GGYRCC 1 cut(s) 5
BanII GRGCYC 1 cut(s) 271
BbsI GAAGAC 1 cut(s) 121
BbvI GCAGC 1 cut(s) 66
BccI CCATC 1 cut(s) 103
BcoDI GTCTC 2 cut(s) 90, 98
BfoI RGCGCY 1 cut(s) 40
BisI GCNGC 2 cut(s) 80, 252
BlsI GCNGC 2 cut(s) 81, 253
BmeT110I CYCGRG 1 cut(s) 101
BmgBI CACGTC 1 cut(s) 142
BmiI GGNNCC 2 cut(s) 7, 241
BmsI GCATC 1 cut(s) 66
BpiI GAAGAC 1 cut(s) 121
BsaI GGTCTC 1 cut(s) 90
BsaWI WCCGGW 1 cut(s) 19
Bsc4I CCNNNNNNNGG 2 cut(s) 208, 261
Bse118I RCCGGY 1 cut(s) 113
Bse1I ACTGG 1 cut(s) 50
BseAI TCCGGA 1 cut(s) 19
BseLI CCNNNNNNNGG 2 cut(s) 208, 261
BseMII CTCAG 2 cut(s) 47, 101
BseNI ACTGG 1 cut(s) 50
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 1 cut(s) 66
Bsh1236I CGCG 3 cut(s) 65, 173, 302
BshFI GGCC 2 cut(s) 113, 254
BshNI GGYRCC 1 cut(s) 5
BsiHKCI CYCGRG 1 cut(s) 101
BsiSI CCGG 3 cut(s) 20, 40, 114
BslI CCNNNNNNNGG 2 cut(s) 208, 261
BsmAI GTCTC 2 cut(s) 90, 98
BsmBI CGTCTC 1 cut(s) 98
BsnI GGCC 2 cut(s) 113, 254
Bso31I GGTCTC 1 cut(s) 90
BsoBI CYCGRG 1 cut(s) 101
Bsp1286I GDGCHC 2 cut(s) 8, 271
Bsp13I TCCGGA 1 cut(s) 19
Bsp143I GATC 3 cut(s) 136, 165, 190
Bsp68I TCGCGA 1 cut(s) 302
BspACI CCGC 5 cut(s) 27, 82, 171, 199, 251
BspANI GGCC 2 cut(s) 113, 254
BspCNI CTCAG 2 cut(s) 46, 100
BspEI TCCGGA 1 cut(s) 19
BspFNI CGCG 3 cut(s) 65, 173, 302
BspLI GGNNCC 2 cut(s) 7, 241
BspPI GGATC 1 cut(s) 173
BspT107I GGYRCC 1 cut(s) 5
BspTNI GGTCTC 1 cut(s) 90
BsrFI RCCGGY 1 cut(s) 113
BsrI ACTGG 1 cut(s) 50
BssAI RCCGGY 1 cut(s) 113
BssMI GATC 3 cut(s) 136, 165, 190
Bst4CI ACNGT 1 cut(s) 324
Bst6I CTCTTC 1 cut(s) 150
BstC8I GCNNGC 2 cut(s) 69, 115
BstDEI CTNAG 4 cut(s) 33, 87, 272, 317
BstFNI CGCG 3 cut(s) 65, 173, 302
BstH2I RGCGCY 1 cut(s) 40
BstHHI GCGC 2 cut(s) 39, 67
BstKTI GATC 3 cut(s) 139, 168, 193
BstMAI GTCTC 2 cut(s) 90, 98
BstMBI GATC 3 cut(s) 136, 165, 190
BstMWI GCNNNNNNNGC 2 cut(s) 88, 299
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 3 cut(s) 65, 173, 302
BstV1I GCAGC 1 cut(s) 66
BstV2I GAAGAC 1 cut(s) 121
BstX2I RGATCY 1 cut(s) 165
BstYI RGATCY 1 cut(s) 165
BsuRI GGCC 2 cut(s) 113, 254
BtrI CACGTC 1 cut(s) 142
BtuMI TCGCGA 1 cut(s) 302
Cac8I GCNNGC 2 cut(s) 69, 115
CaiI CAGNNNCTG 1 cut(s) 79
CfoI GCGC 2 cut(s) 39, 67
Cfr10I RCCGGY 1 cut(s) 113
CviJI RGCY 6 cut(s) 16, 113, 240, 254, 269, 293
CviKI_1 RGCY 6 cut(s) 16, 113, 240, 254, 269, 293
DdeI CTNAG 4 cut(s) 33, 87, 272, 317
DpnI GATC 3 cut(s) 138, 167, 192
DpnII GATC 3 cut(s) 136, 165, 190
DrdI GACNNNNNNGTC 1 cut(s) 125
DseDI GACNNNNNNGTC 1 cut(s) 125
EaeI YGGCCR 2 cut(s) 111, 252
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
EciI GGCGGA 2 cut(s) 16, 188
Eco24I GRGCYC 1 cut(s) 271
Eco31I GGTCTC 1 cut(s) 90
Eco88I CYCGRG 1 cut(s) 101
EcoT38I GRGCYC 1 cut(s) 271
Esp3I CGTCTC 1 cut(s) 98
FaiI YATR 2 cut(s) 278, 297
FalI AAGNNNNNCTT 2 cut(s) 200, 232
Fnu4HI GCNGC 2 cut(s) 80, 252
FriOI GRGCYC 1 cut(s) 271
Fsp4HI GCNGC 2 cut(s) 80, 252
GlaI GCGC 2 cut(s) 38, 66
GluI GCNGC 2 cut(s) 80, 252
HaeII RGCGCY 1 cut(s) 40
HaeIII GGCC 2 cut(s) 113, 254
HapII CCGG 3 cut(s) 20, 40, 114
HhaI GCGC 2 cut(s) 39, 67
Hin6I GCGC 2 cut(s) 37, 65
HinP1I GCGC 2 cut(s) 37, 65
HinfI GANTC 1 cut(s) 23
HpaII CCGG 3 cut(s) 20, 40, 114
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 2 cut(s) 146, 320
Hpy188III TCNNGA 3 cut(s) 20, 103, 301
Hpy8I GTNNAC 1 cut(s) 214
HpyAV CCTTC 2 cut(s) 202, 230
HpyCH4III ACNGT 1 cut(s) 324
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 1 cut(s) 151
HpyF10VI GCNNNNNNNGC 2 cut(s) 88, 299
HpyF3I CTNAG 4 cut(s) 33, 87, 272, 317
HpySE526I ACGT 1 cut(s) 141
HspAI GCGC 2 cut(s) 37, 65
Kpn2I TCCGGA 1 cut(s) 19
KroI GCCGGC 1 cut(s) 113
KroNI GCCGGC 1 cut(s) 115
Kzo9I GATC 3 cut(s) 136, 165, 190
LmnI GCTCC 2 cut(s) 245, 280
LpnPI CCDG 6 cut(s) 31, 33, 53, 53, 127, 215
Lsp1109I GCAGC 1 cut(s) 66
LweI GCATC 1 cut(s) 66
MaeII ACGT 1 cut(s) 141
MalI GATC 3 cut(s) 138, 167, 192
MboI GATC 3 cut(s) 136, 165, 190
MboII GAAGA 3 cut(s) 49, 121, 167
MflI RGATCY 1 cut(s) 165
MhlI GDGCHC 2 cut(s) 8, 271
MluCI AATT 1 cut(s) 221
MlyI GAGTC 1 cut(s) 17
MnlI CCTC 4 cut(s) 40, 110, 241, 283
MroI TCCGGA 1 cut(s) 19
MroNI GCCGGC 1 cut(s) 113
MroXI GAANNNNTTC 1 cut(s) 56
MspI CCGG 3 cut(s) 20, 40, 114
MvnI CGCG 3 cut(s) 65, 173, 302
MwoI GCNNNNNNNGC 2 cut(s) 88, 299
NaeI GCCGGC 1 cut(s) 115
NdeII GATC 3 cut(s) 136, 165, 190
NgoMIV GCCGGC 1 cut(s) 113
NlaIV GGNNCC 2 cut(s) 7, 241
NruI TCGCGA 1 cut(s) 302
PaeR7I CTCGAG 1 cut(s) 101
PdiI GCCGGC 1 cut(s) 115
PdmI GAANNNNTTC 1 cut(s) 56
PkrI GCNGC 2 cut(s) 81, 253
PleI GAGTC 1 cut(s) 17
PpsI GAGTC 1 cut(s) 17
PspN4I GGNNCC 2 cut(s) 7, 241
PstNI CAGNNNCTG 1 cut(s) 79
PsuI RGATCY 1 cut(s) 165
RruI TCGCGA 1 cut(s) 302
SatI GCNGC 2 cut(s) 80, 252
Sau3AI GATC 3 cut(s) 136, 165, 190
SchI GAGTC 1 cut(s) 17
SduI GDGCHC 2 cut(s) 8, 271
SetI ASST 4 cut(s) 18, 102, 144, 213
SfaNI GCATC 1 cut(s) 66
Sfr274I CTCGAG 1 cut(s) 101
SlaI CTCGAG 1 cut(s) 101
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
Sse9I AATT 1 cut(s) 221
SsiI CCGC 5 cut(s) 27, 82, 171, 199, 251
TaaI ACNGT 1 cut(s) 324
TaiI ACGT 1 cut(s) 144
TaqI TCGA 2 cut(s) 95, 102
TasI AATT 1 cut(s) 221
TauI GCSGC 1 cut(s) 254
TseI GCWGC 1 cut(s) 79
TspDTI ATGAA 2 cut(s) 167, 221
TspGWI ACGGA 1 cut(s) 232
XhoI CTCGAG 1 cut(s) 101
XmnI GAANNNNTTC 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.