MD08G1013200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006897
Physical Location & Seq
Reverse (-)
968129 .. 968993
865 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1013200.v1.1.491

Sequence Viewer

Length: 489 bp
ATGGAAAATGATTACAAAACTAAACTGAAGAGCAAGAATTCTGAACTCGAGCAGGCGAATGCAAAGATACAAACTCTTCTTGCCAGTATGGAGCAGTTACAGTCCTCAAGTAATGGAAAGGATGACAAGATTGGAATATTAATAAGTAAAGTTGCTAAGATGGAGGCTGATTCAAACAAGTTCAAGGAGGAAATTTCGAAACTTTCGAAGGAGTTGGAAACATTAAGAAATTCCACAAGCACTTCGTCTACTCCTGTTTTGAACCGGTGTACAACAAGAACTAGAGGCAAGAGTAGTGTTAATGTAACTGTGAAGAAAGATTCGTCGTCTGCACAACGTCCTCATCCTTCAAAGGACACCAAAAAGGGGAGCGGCAGTACAAAGAGAGACGCAGATGATGTTATAACCATTACCGAGACTCCAAAGTTGTTCTCATCCAGATTTAAAGTACCCAAGTTGAAGAATGCATCATCACCCGGTGTAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

17.79

Weight (kDa)

9.9

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FliJ PF02050 5 - 79 8.1e-06 Flagellar FliJ protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 404
AccBSI CCGCTC 1 cut(s) 372
AccI GTMKAC 1 cut(s) 248
AciI CCGC 1 cut(s) 372
AcsI RAATTY 3 cut(s) 37, 192, 229
AcuI CTGAAG 1 cut(s) 47
AdeI CACNNNGTG 1 cut(s) 479
AfaI GTAC 3 cut(s) 271, 379, 450
AfiI CCNNNNNNNGG 1 cut(s) 366
AgeI ACCGGT 1 cut(s) 264
AgsI TTSAA 5 cut(s) 174, 184, 262, 351, 460
Alw26I GTCTC 2 cut(s) 381, 410
Ama87I CYCGRG 1 cut(s) 47
ApoI RAATTY 3 cut(s) 37, 192, 229
AseI ATTAAT 1 cut(s) 140
AsiGI ACCGGT 1 cut(s) 264
AsuC2I CCSGG 1 cut(s) 477
AsuHPI GGTGA 1 cut(s) 465
AsuII TTCGAA 2 cut(s) 197, 206
AvaI CYCGRG 1 cut(s) 47
BccI CCATC 1 cut(s) 154
BcnI CCSGG 1 cut(s) 477
BcoDI GTCTC 2 cut(s) 381, 410
BfaI CTAG 1 cut(s) 282
BisI GCNGC 1 cut(s) 373
BlsI GCNGC 1 cut(s) 374
Bme1390I CCNGG 1 cut(s) 477
BmeT110I CYCGRG 1 cut(s) 47
BmrFI CCNGG 1 cut(s) 477
BmsI GCATC 1 cut(s) 476
Bpu14I TTCGAA 2 cut(s) 197, 206
BpuEI CTTGAG 1 cut(s) 91
BpuMI CCSGG 1 cut(s) 477
BsaWI WCCGGW 1 cut(s) 264
BsaXI ACNNNNNCTCC 4 cut(s) 361, 391, 403, 433
Bsc4I CCNNNNNNNGG 1 cut(s) 366
Bse118I RCCGGY 1 cut(s) 264
Bse1I ACTGG 1 cut(s) 84
BseGI GGATG 3 cut(s) 127, 343, 434
BseLI CCNNNNNNNGG 1 cut(s) 366
BseNI ACTGG 1 cut(s) 84
BsgI GTGCAG 1 cut(s) 315
BshTI ACCGGT 1 cut(s) 264
BsiHKCI CYCGRG 1 cut(s) 47
BsiSI CCGG 2 cut(s) 265, 477
BslI CCNNNNNNNGG 1 cut(s) 366
BsmAI GTCTC 2 cut(s) 381, 410
BsmBI CGTCTC 1 cut(s) 381
BsmI GAATGC 2 cut(s) 64, 469
BsoBI CYCGRG 1 cut(s) 47
Bsp119I TTCGAA 2 cut(s) 197, 206
Bsp1407I TGTACA 1 cut(s) 269
BspACI CCGC 1 cut(s) 372
BspQI GCTCTTC 1 cut(s) 23
BspT104I TTCGAA 2 cut(s) 197, 206
BsrBI CCGCTC 1 cut(s) 372
BsrFI RCCGGY 1 cut(s) 264
BsrGI TGTACA 1 cut(s) 269
BsrI ACTGG 1 cut(s) 84
BssAI RCCGGY 1 cut(s) 264
Bst4CI ACNGT 2 cut(s) 102, 310
Bst6I CTCTTC 2 cut(s) 23, 81
BstAUI TGTACA 1 cut(s) 269
BstBI TTCGAA 2 cut(s) 197, 206
BstC8I GCNNGC 1 cut(s) 54
BstDEI CTNAG 1 cut(s) 156
BstF5I GGATG 3 cut(s) 127, 343, 434
BstMAI GTCTC 2 cut(s) 381, 410
BstSCI CCNGG 1 cut(s) 475
BtsCI GGATG 3 cut(s) 127, 343, 434
Cac8I GCNNGC 1 cut(s) 54
Cfr10I RCCGGY 1 cut(s) 264
CseI GACGC 1 cut(s) 398
Csp6I GTAC 3 cut(s) 270, 378, 449
CspAI ACCGGT 1 cut(s) 264
CviJI RGCY 1 cut(s) 167
CviKI_1 RGCY 1 cut(s) 167
CviQI GTAC 3 cut(s) 270, 378, 449
DdeI CTNAG 1 cut(s) 156
DraI TTTAAA 1 cut(s) 445
DraIII CACNNNGTG 1 cut(s) 479
Eam1104I CTCTTC 2 cut(s) 23, 81
EarI CTCTTC 2 cut(s) 23, 81
Eco57I CTGAAG 1 cut(s) 47
Eco88I CYCGRG 1 cut(s) 47
EcoRI GAATTC 1 cut(s) 37
EcoT22I ATGCAT 1 cut(s) 469
Esp3I CGTCTC 1 cut(s) 381
FaiI YATR 2 cut(s) 89, 404
FblI GTMKAC 1 cut(s) 248
Fnu4HI GCNGC 1 cut(s) 373
FokI GGATG 3 cut(s) 134, 330, 421
Fsp4HI GCNGC 1 cut(s) 373
FspBI CTAG 1 cut(s) 282
GluI GCNGC 1 cut(s) 373
HapII CCGG 2 cut(s) 265, 477
HgaI GACGC 1 cut(s) 398
HinfI GANTC 3 cut(s) 170, 320, 418
HpaII CCGG 2 cut(s) 265, 477
HphI GGTGA 1 cut(s) 465
Hpy166II GTNNAC 2 cut(s) 249, 270
Hpy188I TCNGA 1 cut(s) 43
Hpy188III TCNNGA 1 cut(s) 438
Hpy8I GTNNAC 2 cut(s) 249, 270
Hpy99I CGWCG 1 cut(s) 328
HpyAV CCTTC 2 cut(s) 202, 357
HpyCH4III ACNGT 2 cut(s) 102, 310
HpyCH4IV ACGT 1 cut(s) 337
HpyCH4V TGCA 3 cut(s) 62, 332, 467
HpyF3I CTNAG 1 cut(s) 156
HpySE526I ACGT 1 cut(s) 337
LguI GCTCTTC 1 cut(s) 23
LmnI GCTCC 2 cut(s) 91, 369
LpnPI CCDG 5 cut(s) 38, 97, 267, 278, 451
LweI GCATC 1 cut(s) 476
MaeI CTAG 1 cut(s) 282
MaeII ACGT 1 cut(s) 337
MaeIII GTNAC 2 cut(s) 96, 304
MbiI CCGCTC 1 cut(s) 372
MboII GAAGA 4 cut(s) 40, 68, 325, 472
MluCI AATT 3 cut(s) 37, 192, 229
MlyI GAGTC 1 cut(s) 412
MmeI TCCRAC 1 cut(s) 195
MnlI CCTC 5 cut(s) 115, 157, 181, 278, 351
Mph1103I ATGCAT 1 cut(s) 469
MseI TTAA 4 cut(s) 140, 224, 300, 444
MspI CCGG 2 cut(s) 265, 477
MspR9I CCNGG 1 cut(s) 477
Mva1269I GAATGC 2 cut(s) 64, 469
NciI CCSGG 1 cut(s) 477
NsiI ATGCAT 1 cut(s) 469
NspV TTCGAA 2 cut(s) 197, 206
PaeR7I CTCGAG 1 cut(s) 47
PciSI GCTCTTC 1 cut(s) 23
PcsI WCGNNNNNNNCGW 1 cut(s) 203
PctI GAATGC 2 cut(s) 64, 469
PfeI GAWTC 2 cut(s) 170, 320
PinAI ACCGGT 1 cut(s) 264
PkrI GCNGC 1 cut(s) 374
PleI GAGTC 1 cut(s) 412
PpsI GAGTC 1 cut(s) 412
PshBI ATTAAT 1 cut(s) 140
PsiI TTATAA 1 cut(s) 404
PspXI VCTCGAGB 1 cut(s) 47
RsaI GTAC 3 cut(s) 271, 379, 450
RsaNI GTAC 3 cut(s) 270, 378, 449
SapI GCTCTTC 1 cut(s) 23
SaqAI TTAA 4 cut(s) 140, 224, 300, 444
SatI GCNGC 1 cut(s) 373
SchI GAGTC 1 cut(s) 412
ScrFI CCNGG 1 cut(s) 477
SetI ASST 1 cut(s) 340
SfaNI GCATC 1 cut(s) 476
Sfr274I CTCGAG 1 cut(s) 47
SfuI TTCGAA 2 cut(s) 197, 206
SlaI CTCGAG 1 cut(s) 47
SmlI CTYRAG 2 cut(s) 47, 106
SmoI CTYRAG 2 cut(s) 47, 106
Sse9I AATT 3 cut(s) 37, 192, 229
SsiI CCGC 1 cut(s) 372
SspI AATATT 1 cut(s) 138
SspMI CTAG 1 cut(s) 282
StyD4I CCNGG 1 cut(s) 475
TaaI ACNGT 2 cut(s) 102, 310
TaiI ACGT 1 cut(s) 340
TaqI TCGA 3 cut(s) 48, 197, 206
TasI AATT 3 cut(s) 37, 192, 229
TatI WGTACW 2 cut(s) 269, 377
TauI GCSGC 1 cut(s) 375
TfiI GAWTC 2 cut(s) 170, 320
Tru1I TTAA 4 cut(s) 140, 224, 300, 444
Tru9I TTAA 4 cut(s) 140, 224, 300, 444
VspI ATTAAT 1 cut(s) 140
XapI RAATTY 3 cut(s) 37, 192, 229
XhoI CTCGAG 1 cut(s) 47
XmiI GTMKAC 1 cut(s) 248
XspI CTAG 1 cut(s) 282
Zsp2I ATGCAT 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.