pycom08g01130

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0006897
Physical Location & Seq
Reverse (-)
865350 .. 866888
1539 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g01130.2

Sequence Viewer

Length: 936 bp
ATGGGCACCAAAGAGCTTCTGGACCCCCCCTCTCAGCGCCGGAACTGGCACAACATATTCAACGCGCTGGTACAGATGCTGCGGTCTCAGCAATCGAACCTCGAGACGATGGCCGCCGACAGAAAAGTCTTCAAAGATCGCGTCAGAGTGCAAGAAGAGAAGTGGATCTCCGCGTTTCATCTCTTGGGAGATCAAATCCGCCAGATGAAGGTGGACTTGGAATTGAAAGAAATGGAAGGCTCCGTTGAGTCGGCCAAGTTAGGGTGGGCTCTTAGCATGAAGCAACAAGAGGCTTATATCGCGAAACTCAAGTTAGAATACTCAGACAGTGAGGTAGAAGGTTTTAAAGCATGGTTTGACCTACACTCCAACAAAAATACCACCTTAAAGCAGCAATGTGATAAGCTTGCTTCCGAAAAGAGCTTTGCGTGGCATCAGTACGACCTTATGGAAAATGATTACAAAACTAAACTGAAGAGCAAGAATTCTGAACTCGAGCAGGCGAATGCAAAGATACAAACTCTTCTCGCCAGTATGGAGCAGTTACAGTCCTCAAGTACCGAAAAGGATGACAAGATTGGAATTTTAATAAGTAAAGTTGCTAAGATGGAGACTGATTCAAACAATTTCAAGGAGGAAATTTCTAAACTTTCGAAGGAGTTGGAAACATTAAGAAATTCCGCAAGTACTTCGTCTACTCCTGTTTTGAACCGGTGTACAACAAGAACTAGAGGCAAGAGTAGTGTTAATGTAACTGTGAAGAAAGATTCGTCAGCTGTACAACGTCCTCATCCTTCAAAGGACACCAAAAAGGGGAGCGGCAGTTCAAAGAGAAACGCAGATGATGTTATAACCATTACCGAGACTCCAAAGTTGTTCTCATCCAGATTTAAAGTACCCAAGTTGAAGAATGCATCATCACCCAGTGTAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

312

Amino Acids

35.34

Weight (kDa)

9.46

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 851
AasI GACNNNNNNGTC 1 cut(s) 125
AccB1I GGYRCC 1 cut(s) 5
AccBSI CCGCTC 1 cut(s) 819
AccI GTMKAC 1 cut(s) 695
AccII CGCG 4 cut(s) 65, 141, 173, 302
AciI CCGC 6 cut(s) 82, 114, 171, 199, 681, 819
AclWI GGATC 1 cut(s) 173
AcoI YGGCCR 2 cut(s) 111, 252
AcsI RAATTY 4 cut(s) 484, 582, 639, 676
AcuI CTGAAG 1 cut(s) 494
AdeI CACNNNGTG 1 cut(s) 926
AfaI GTAC 7 cut(s) 72, 440, 559, 688, 718, 780, 897
AfiI CCNNNNNNNGG 3 cut(s) 208, 261, 813
AgeI ACCGGT 1 cut(s) 711
AgsI TTSAA 9 cut(s) 61, 133, 226, 621, 631, 709, 798, 828, 907
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AloI GAACNNNNNNTCC 2 cut(s) 808, 840
AluBI AGCT 4 cut(s) 16, 406, 423, 776
AluI AGCT 4 cut(s) 16, 406, 423, 776
Alw26I GTCTC 4 cut(s) 90, 98, 605, 857
AlwI GGATC 1 cut(s) 173
AlwNI CAGNNNCTG 1 cut(s) 79
Ama87I CYCGRG 2 cut(s) 101, 494
AoxI GGCC 2 cut(s) 111, 252
ApeKI GCWGC 2 cut(s) 79, 391
ApoI RAATTY 4 cut(s) 484, 582, 639, 676
ArsI GACNNNNNNTTYG 2 cut(s) 126, 158
AsiGI ACCGGT 1 cut(s) 711
AspLEI GCGC 2 cut(s) 39, 67
AspS9I GGNCC 1 cut(s) 22
AsuHPI GGTGA 1 cut(s) 912
AsuII TTCGAA 1 cut(s) 653
AvaI CYCGRG 2 cut(s) 101, 494
AvaII GGWCC 1 cut(s) 22
BaeGI GKGCMC 1 cut(s) 8
BanI GGYRCC 1 cut(s) 5
BanII GRGCYC 1 cut(s) 271
BbsI GAAGAC 1 cut(s) 121
BbvI GCAGC 2 cut(s) 66, 403
BccI CCATC 2 cut(s) 103, 601
BcgI CGANNNNNNTGC 2 cut(s) 672, 706
BcoDI GTCTC 4 cut(s) 90, 98, 605, 857
BfaI CTAG 1 cut(s) 729
BfoI RGCGCY 1 cut(s) 40
BisI GCNGC 4 cut(s) 80, 114, 392, 820
BlsI GCNGC 4 cut(s) 81, 115, 393, 821
BmcAI AGTACT 1 cut(s) 688
Bme18I GGWCC 1 cut(s) 22
BmeT110I CYCGRG 2 cut(s) 101, 494
BmgT120I GGNCC 1 cut(s) 22
BmiI GGNNCC 3 cut(s) 7, 24, 241
BmrI ACTGGG 1 cut(s) 918
BmsI GCATC 3 cut(s) 66, 442, 923
BmuI ACTGGG 1 cut(s) 918
BpiI GAAGAC 1 cut(s) 121
Bpu14I TTCGAA 1 cut(s) 653
BpuEI CTTGAG 2 cut(s) 293, 538
BsaI GGTCTC 1 cut(s) 90
BsaWI WCCGGW 1 cut(s) 711
BsaXI ACNNNNNCTCC 6 cut(s) 350, 380, 808, 838, 850, 880
Bsc4I CCNNNNNNNGG 3 cut(s) 208, 261, 813
Bse118I RCCGGY 1 cut(s) 711
Bse1I ACTGG 3 cut(s) 50, 531, 924
Bse3DI GCAATG 1 cut(s) 401
BseGI GGATG 3 cut(s) 574, 790, 881
BseLI CCNNNNNNNGG 3 cut(s) 208, 261, 813
BseMI GCAATG 1 cut(s) 401
BseMII CTCAG 3 cut(s) 47, 101, 336
BseNI ACTGG 3 cut(s) 50, 531, 924
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 2 cut(s) 66, 403
Bsh1236I CGCG 4 cut(s) 65, 141, 173, 302
BshFI GGCC 2 cut(s) 113, 254
BshNI GGYRCC 1 cut(s) 5
BshTI ACCGGT 1 cut(s) 711
BsiHKCI CYCGRG 2 cut(s) 101, 494
BsiSI CCGG 2 cut(s) 40, 712
BslI CCNNNNNNNGG 3 cut(s) 208, 261, 813
BsmAI GTCTC 4 cut(s) 90, 98, 605, 857
BsmBI CGTCTC 1 cut(s) 98
BsmI GAATGC 2 cut(s) 511, 916
BsnI GGCC 2 cut(s) 113, 254
Bso31I GGTCTC 1 cut(s) 90
BsoBI CYCGRG 2 cut(s) 101, 494
Bsp119I TTCGAA 1 cut(s) 653
Bsp1286I GDGCHC 2 cut(s) 8, 271
Bsp1407I TGTACA 2 cut(s) 716, 778
Bsp143I GATC 3 cut(s) 136, 165, 190
Bsp68I TCGCGA 1 cut(s) 302
BspACI CCGC 6 cut(s) 82, 114, 171, 199, 681, 819
BspANI GGCC 2 cut(s) 113, 254
BspCNI CTCAG 3 cut(s) 46, 100, 335
BspFNI CGCG 4 cut(s) 65, 141, 173, 302
BspLI GGNNCC 3 cut(s) 7, 24, 241
BspPI GGATC 1 cut(s) 173
BspQI GCTCTTC 1 cut(s) 470
BspT104I TTCGAA 1 cut(s) 653
BspT107I GGYRCC 1 cut(s) 5
BspTNI GGTCTC 1 cut(s) 90
BsrBI CCGCTC 1 cut(s) 819
BsrDI GCAATG 1 cut(s) 401
BsrFI RCCGGY 1 cut(s) 711
BsrGI TGTACA 2 cut(s) 716, 778
BsrI ACTGG 3 cut(s) 50, 531, 924
BssAI RCCGGY 1 cut(s) 711
BssMI GATC 3 cut(s) 136, 165, 190
Bst4CI ACNGT 3 cut(s) 329, 549, 757
Bst6I CTCTTC 3 cut(s) 150, 470, 528
BstAUI TGTACA 2 cut(s) 716, 778
BstBI TTCGAA 1 cut(s) 653
BstC8I GCNNGC 2 cut(s) 408, 501
BstDEI CTNAG 5 cut(s) 33, 87, 272, 322, 603
BstF5I GGATG 3 cut(s) 574, 790, 881
BstFNI CGCG 4 cut(s) 65, 141, 173, 302
BstH2I RGCGCY 1 cut(s) 40
BstHHI GCGC 2 cut(s) 39, 67
BstKTI GATC 3 cut(s) 139, 168, 193
BstMAI GTCTC 4 cut(s) 90, 98, 605, 857
BstMBI GATC 3 cut(s) 136, 165, 190
BstMWI GCNNNNNNNGC 2 cut(s) 88, 299
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 4 cut(s) 65, 141, 173, 302
BstV1I GCAGC 2 cut(s) 66, 403
BstV2I GAAGAC 1 cut(s) 121
BstX2I RGATCY 1 cut(s) 165
BstYI RGATCY 1 cut(s) 165
BsuRI GGCC 2 cut(s) 113, 254
BtsCI GGATG 3 cut(s) 574, 790, 881
BtsIMutI CAGTG 2 cut(s) 334, 931
BtuMI TCGCGA 1 cut(s) 302
Cac8I GCNNGC 2 cut(s) 408, 501
CaiI CAGNNNCTG 1 cut(s) 79
CfoI GCGC 2 cut(s) 39, 67
Cfr10I RCCGGY 1 cut(s) 711
Cfr13I GGNCC 1 cut(s) 22
CseI GACGC 1 cut(s) 130
Csp6I GTAC 7 cut(s) 71, 439, 558, 687, 717, 779, 896
CspAI ACCGGT 1 cut(s) 711
CviAII CATG 2 cut(s) 277, 351
CviJI RGCY 9 cut(s) 16, 113, 240, 254, 269, 293, 406, 423, 776
CviKI_1 RGCY 9 cut(s) 16, 113, 240, 254, 269, 293, 406, 423, 776
CviQI GTAC 7 cut(s) 71, 439, 558, 687, 717, 779, 896
DdeI CTNAG 5 cut(s) 33, 87, 272, 322, 603
DpnI GATC 3 cut(s) 138, 167, 192
DpnII GATC 3 cut(s) 136, 165, 190
DraI TTTAAA 2 cut(s) 346, 892
DraIII CACNNNGTG 1 cut(s) 926
DrdI GACNNNNNNGTC 1 cut(s) 125
DseDI GACNNNNNNGTC 1 cut(s) 125
EaeI YGGCCR 2 cut(s) 111, 252
Eam1104I CTCTTC 3 cut(s) 150, 470, 528
EarI CTCTTC 3 cut(s) 150, 470, 528
EciI GGCGGA 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 271
Eco31I GGTCTC 1 cut(s) 90
Eco47I GGWCC 1 cut(s) 22
Eco57I CTGAAG 1 cut(s) 494
Eco88I CYCGRG 2 cut(s) 101, 494
EcoRI GAATTC 1 cut(s) 484
EcoT22I ATGCAT 1 cut(s) 916
EcoT38I GRGCYC 1 cut(s) 271
Esp3I CGTCTC 1 cut(s) 98
FaeI CATG 2 cut(s) 280, 354
FaiI YATR 7 cut(s) 56, 278, 297, 352, 449, 536, 851
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FatI CATG 2 cut(s) 276, 350
FblI GTMKAC 1 cut(s) 695
Fnu4HI GCNGC 4 cut(s) 80, 114, 392, 820
FokI GGATG 3 cut(s) 581, 777, 868
FriOI GRGCYC 1 cut(s) 271
Fsp4HI GCNGC 4 cut(s) 80, 114, 392, 820
FspBI CTAG 1 cut(s) 729
GlaI GCGC 2 cut(s) 38, 66
GluI GCNGC 4 cut(s) 80, 114, 392, 820
HaeII RGCGCY 1 cut(s) 40
HaeIII GGCC 2 cut(s) 113, 254
HapII CCGG 2 cut(s) 40, 712
HgaI GACGC 1 cut(s) 130
HhaI GCGC 2 cut(s) 39, 67
Hin1II CATG 2 cut(s) 280, 354
Hin6I GCGC 2 cut(s) 37, 65
HinP1I GCGC 2 cut(s) 37, 65
HindIII AAGCTT 1 cut(s) 404
HinfI GANTC 4 cut(s) 248, 617, 767, 865
HpaII CCGG 2 cut(s) 40, 712
HphI GGTGA 1 cut(s) 912
Hpy166II GTNNAC 3 cut(s) 214, 696, 717
Hpy188I TCNGA 4 cut(s) 146, 325, 415, 490
Hpy188III TCNNGA 4 cut(s) 20, 103, 301, 885
Hpy8I GTNNAC 3 cut(s) 214, 696, 717
HpyAV CCTTC 5 cut(s) 202, 230, 332, 649, 804
HpyCH4III ACNGT 3 cut(s) 329, 549, 757
HpyCH4IV ACGT 1 cut(s) 784
HpyCH4V TGCA 3 cut(s) 151, 509, 914
HpyF10VI GCNNNNNNNGC 2 cut(s) 88, 299
HpyF3I CTNAG 5 cut(s) 33, 87, 272, 322, 603
HpySE526I ACGT 1 cut(s) 784
Hsp92II CATG 2 cut(s) 280, 354
HspAI GCGC 2 cut(s) 37, 65
Kzo9I GATC 3 cut(s) 136, 165, 190
LguI GCTCTTC 1 cut(s) 470
LmnI GCTCC 3 cut(s) 245, 538, 816
Lsp1109I GCAGC 2 cut(s) 66, 403
LweI GCATC 3 cut(s) 66, 442, 923
MaeI CTAG 1 cut(s) 729
MaeII ACGT 1 cut(s) 784
MaeIII GTNAC 2 cut(s) 543, 751
MalI GATC 3 cut(s) 138, 167, 192
MbiI CCGCTC 1 cut(s) 819
MboI GATC 3 cut(s) 136, 165, 190
MboII GAAGA 6 cut(s) 121, 167, 487, 515, 772, 919
MflI RGATCY 1 cut(s) 165
MhlI GDGCHC 2 cut(s) 8, 271
MluCI AATT 6 cut(s) 221, 484, 582, 625, 639, 676
MlyI GAGTC 2 cut(s) 257, 859
MmeI TCCRAC 2 cut(s) 393, 642
MnlI CCTC 8 cut(s) 40, 110, 283, 325, 562, 628, 725, 798
Mph1103I ATGCAT 1 cut(s) 916
MseI TTAA 6 cut(s) 345, 386, 587, 671, 747, 891
MspA1I CMGCKG 1 cut(s) 776
MspI CCGG 2 cut(s) 40, 712
Mva1269I GAATGC 2 cut(s) 511, 916
MvnI CGCG 4 cut(s) 65, 141, 173, 302
MwoI GCNNNNNNNGC 2 cut(s) 88, 299
NdeII GATC 3 cut(s) 136, 165, 190
NlaIII CATG 2 cut(s) 280, 354
NlaIV GGNNCC 3 cut(s) 7, 24, 241
NruI TCGCGA 1 cut(s) 302
NsiI ATGCAT 1 cut(s) 916
NspV TTCGAA 1 cut(s) 653
PaeR7I CTCGAG 2 cut(s) 101, 494
PciSI GCTCTTC 1 cut(s) 470
PctI GAATGC 2 cut(s) 511, 916
PfeI GAWTC 2 cut(s) 617, 767
PinAI ACCGGT 1 cut(s) 711
PkrI GCNGC 4 cut(s) 81, 115, 393, 821
PleI GAGTC 2 cut(s) 256, 859
PpsI GAGTC 2 cut(s) 256, 859
PsiI TTATAA 1 cut(s) 851
PspN4I GGNNCC 3 cut(s) 7, 24, 241
PspPI GGNCC 1 cut(s) 22
PspXI VCTCGAGB 1 cut(s) 494
PstNI CAGNNNCTG 1 cut(s) 79
PsuI RGATCY 1 cut(s) 165
PvuII CAGCTG 1 cut(s) 776
RruI TCGCGA 1 cut(s) 302
RsaI GTAC 7 cut(s) 72, 440, 559, 688, 718, 780, 897
RsaNI GTAC 7 cut(s) 71, 439, 558, 687, 717, 779, 896
SapI GCTCTTC 1 cut(s) 470
SaqAI TTAA 6 cut(s) 345, 386, 587, 671, 747, 891
SatI GCNGC 4 cut(s) 80, 114, 392, 820
Sau3AI GATC 3 cut(s) 136, 165, 190
Sau96I GGNCC 1 cut(s) 22
ScaI AGTACT 1 cut(s) 688
SchI GAGTC 2 cut(s) 257, 859
SduI GDGCHC 2 cut(s) 8, 271
SfaNI GCATC 3 cut(s) 66, 442, 923
Sfr274I CTCGAG 2 cut(s) 101, 494
SfuI TTCGAA 1 cut(s) 653
SinI GGWCC 1 cut(s) 22
SlaI CTCGAG 2 cut(s) 101, 494
SmlI CTYRAG 4 cut(s) 101, 308, 494, 553
SmoI CTYRAG 4 cut(s) 101, 308, 494, 553
Sse9I AATT 6 cut(s) 221, 484, 582, 625, 639, 676
SsiI CCGC 6 cut(s) 82, 114, 171, 199, 681, 819
SspMI CTAG 1 cut(s) 729
TaaI ACNGT 3 cut(s) 329, 549, 757
TaiI ACGT 1 cut(s) 787
TaqI TCGA 4 cut(s) 95, 102, 495, 653
TasI AATT 6 cut(s) 221, 484, 582, 625, 639, 676
TatI WGTACW 3 cut(s) 686, 716, 778
TauI GCSGC 2 cut(s) 116, 822
TfiI GAWTC 2 cut(s) 617, 767
Tru1I TTAA 6 cut(s) 345, 386, 587, 671, 747, 891
Tru9I TTAA 6 cut(s) 345, 386, 587, 671, 747, 891
TscAI CASTG 2 cut(s) 334, 931
TseI GCWGC 2 cut(s) 79, 391
TspDTI ATGAA 3 cut(s) 167, 221, 293
TspGWI ACGGA 1 cut(s) 232
TspRI CASTG 2 cut(s) 334, 931
VpaK11BI GGWCC 1 cut(s) 22
XapI RAATTY 4 cut(s) 484, 582, 639, 676
XcmI CCANNNNNNNNNTGG 1 cut(s) 16
XhoI CTCGAG 2 cut(s) 101, 494
XmiI GTMKAC 1 cut(s) 695
XspI CTAG 1 cut(s) 729
ZrmI AGTACT 1 cut(s) 688
Zsp2I ATGCAT 1 cut(s) 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.