Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0001793

Physical Location & Seq

Genomic Coordinates Forward (+)

21944959 .. 21950236

Gene Structure 5278 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1104400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2217 bp

ATGTCTTCTGCCCAGGGAAAAGAAAAGCCAGCAGCCATAAAGTTTCAGAAGAGCTACTTTGATGTGTTGGGGTTGTGCTGCGCATCAGAAGTTCCTCTGATAGAGAATATCCTCAAGCCTCTTGAAGGTGTGAAAGAGGTTTCAGTGATTGTGCCTTCCAGAACTGTCATTGTTGTCCATGATAGCCTTCTCATTTCCCAGCTTCAAATCGTTAAGGCACTAAACCAAGCAAGGCTAGAAGCAAATGTGAGAGTGTATGGGGCAGGGGACATTTACAAGAAAACGTGGCCAAGTCCATATGCAATTGGTAGTGGCGTGTTACTGGTTCTATCATTTCTCAAGTACGCATATCGGCCATTAGGGTGGTTGGCCCTTGGAGCCGTGGCCGTTGGCATTTTCCCCGTTGCTTTGAAATGTTTTGCGGCTATTCGGAATTTCAGGTTTCATGACATCAACATTCTTGTTATCATTGCCGTAATTGGGACAATTGCTTTGAAGGACTATACAGAAGCTGCCTCGATTGTCTTTCTGTTCACCATTGCAGAGTGGCTCCAGTCTTCTGCAGGTTACAGGGCAAAAGCAGTGATGTCGTCGTTGATGAGCATGGCCCCCCAAATAGCAGTCTTAGCAGAGAGTGGAGAAGTTGTGGGTGTTGATGAGGTAAAGTTGAACACAATTCTTGCTGTTAAGGCCGGCGAAAAGATACCGATTGATGGAATAGTTGTTGAAGGAAAAGCTGAAGTCGATGAGAAAACACTAACTGGAGAATCCTATCCAGTTGCCAAAGAAAAGGACTCCACTGTTTGGGCAGGCACTATCAATCTTAATGGTTATATTAGCGTGAAAACTACATCATTGGCTGAAGATTGTGCGGTCGCCAAAATGGCAAAACTCATCGAAGAGGCTCAAAACAGCAAATCCAGAACTCAAAGATTCATTGACAAATGTGCAATGTTCTATACCCCAGCGGTCCTGGTCATTTCTGTTTCTATCGCGGTGATTCCAGCTGCATTACATGTTCACAATTGGAGAAAGTGGTTTCACTTAGCTTTGGTTGTTTTAGTGAGCGCCTGTCCGTGCGGTCTCATCCTCTCTACACCCGTTGTGACTTTCTGCACGCTCACAAAAGCGGCAACATCTGGACTTCTGATCAAAGGAGGTGACTACATTGAGATACTTGCTAAAGTCAAGATCATGGCTTTTGACAAAACTGGTACAATAACAAGGGGTGAATTTGTGGTGATGGATTTTCAATCTCTTCGTGATGACATTAGCTTGAACACATTGCTTTACTGGGTTTCAAGCATTGAGAGGAAGTCAAGTCATCCAATGGCAGATGCGCTGGTTGACTATGGAAGATTGTTTTCGGTCGAGCCAAACCCTGAGAATGTGGAGGAGTTTCAAAATTTTCCCGGCGAAGTTCCAACAATTGAAGGTTCGAAAGGTGGGAAGACGATTGGATACATATACTCTGGGGGAGCCCCTGCTGGAGTCTTTATGATATCTGATGCTTGTCGATCTGGGGCTGCAGAGGCTCTCAAGGAACTAAAGAAGTTAGGCATTAAAACAGCTATGCTCACAGGAGATAGTAATGCTGCCGCATTGCATACAAATGAGCAGCTTAAGCAAGCTCTCGAGGTAGTCCAGGCAGAACTTCTACCTGAAGACAAGGCAAGAATCATTAGAGAATTTAAAATGGAAGGAAACACAGCAATGGTTGGAGACGGGATCAATGATGCCCCTGCTTTAGCTACTGCCGATATTGGTATCTCAATGGGCATTTCAGGATCAGCCCTTGCTCAAGAAACTGGGAATATAATTTTACTGTCAAATGACATTAGGAAACTACCGAAAGCAGTCCAACTTGCGAGAAGAGCTAATAGAAAAGTGATTGAGAATGTGGCTTTGTCAATCACTGCTAAGGCTAGTATCCTTGCACTGGGATTTGCAGGGCATCCGCTTGTTTGGGCTGCAGTTCTTGCTGATGCCGGGACATGCTTGCTTGTGATATTCAACAGCATGCTACTCTTACAAGGAACCAACAAGCATGGAGGGAAAACTTCTGCACCACATGGCCATAAACATGGAAGCCATGGACATGGCCATAAACATGGAAGCCATGGACATGGCCATAAACATGGAAGCCATGGACATAGCCATTCTCACAAAAACCAACATTGTTGCTCGGACAGCATACCCGTAAAAGTCTTGCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006829 GO:0006950 GO:0008150 GO:0009506 GO:0009636 GO:0010035 GO:0010038 GO:0010043 GO:0015691 GO:0016020 GO:0030001 GO:0030054 GO:0031090 GO:0032025 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055065 GO:0055069 GO:0055076 GO:0055080 GO:0061687 GO:0065007 GO:0065008 GO:0070838 GO:0071585 GO:0071944 GO:0072507 GO:0072511 GO:0097501 GO:0098588 GO:0098754 GO:0098771 GO:0098805 GO:1990170

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

739

Amino Acids

79.04

Weight (kDa)

8.61

Isoelectric Point (pI)

31.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	212 - 300	2.4e-22	P-type ATPase actuator domain
Hydrolase	PF00702	396 - 587	4e-24	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001793)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19110 AT2G19110 AT2G19110 AT4G30110 AT4G30110 AT4G30120 AT4G30120
fragaria_vesca	FvH4_4g07520 FvH4_4g07520 FvH4_4g07520 FvH4_4g07520 FvH4_6g33520 FvH4_6g33520 FvH4_6g33520 FvH4_6g33520
malus_domestica	MD00G1104400.v1.1 MD01G1183400.v1.1 MD01G1183700.v1.1 MD01G1184000.v1.1 MD01G1184300.v1.1 MD13G1244500.v1.1 MD16G1252700.v1.1
prunus_persica	Prupe.1G078700_v2.0.a1 Prupe.1G078700_v2.0.a1 Prupe.1G078700_v2.0.a1
pyrus_communis	pycom13g21550 pycom16g21330
rosa_chinensis	RchiOBHm_Chr2g0143431 RchiOBHm_Chr4g0400101
rosa_laevigata	RLG00000009214 RLG00000020035
rosa_multiflora	Rmu_sc0000037.1_g000006
rosa_roxburghii	Rroxscaffold_2G00101850 Rroxscaffold_5G00345040 Rroxscaffold_5G00345070
rosa_rugosa	Rorug02G0381400 Rorug04G0022600 Rorug04G0022600
rosa_samantha	Rh2AG434400 Rh2BG442200 Rh2CG420300 Rh2DG452400 Rh4BG096400 Rh4CG107500 Rh4DG093000
rosa_wichuraiana	Rw2G035400 Rw4G008180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	82
Acc36I	ACCTGC	1 cut(s)	554
AccB7I	CCANNNNNTGG	1 cut(s)	804
AccII	CGCG	1 cut(s)	995
AciI	CCGC	8 cut(s)	422, 872, 968, 995, 1080, 1130, 1599, 1958
AclWI	GGATC	2 cut(s)	1736, 1795
AcoI	YGGCCR	6 cut(s)	287, 353, 384, 2074, 2101, 2128
AcsI	RAATTY	4 cut(s)	433, 1232, 1405, 1688
AcuI	CTGAAG	3 cut(s)	759, 882, 1683
AfaI	GTAC	2 cut(s)	344, 1216
AfiI	CCNNNNNNNGG	5 cut(s)	125, 480, 713, 804, 1939
AflII	CTTAAG	1 cut(s)	1622
AflIII	ACRYGT	1 cut(s)	1015
AjnI	CCWGG	3 cut(s)	12, 972, 1644
Alw26I	GTCTC	2 cut(s)	1088, 1716
AlwI	GGATC	2 cut(s)	1736, 1795
AlwNI	CAGNNNCTG	1 cut(s)	512
Ama87I	CYCGRG	1 cut(s)	1634
AoxI	GGCC	9 cut(s)	287, 353, 369, 384, 606, 690, 2074, 2101, 2128
ApeKI	GCWGC	8 cut(s)	32, 78, 512, 1007, 1526, 1595, 1618, 1970
ApoI	RAATTY	4 cut(s)	433, 1232, 1405, 1688
ArsI	GACNNNNNNTTYG	2 cut(s)	475, 507
AspLEI	GCGC	3 cut(s)	83, 1070, 1342
AspS9I	GGNCC	3 cut(s)	370, 607, 970
AsuC2I	CCSGG	2 cut(s)	1413, 1990
AsuHPI	GGTGA	5 cut(s)	526, 1009, 1172, 1241, 1252
AsuII	TTCGAA	1 cut(s)	1439
AvaI	CYCGRG	1 cut(s)	1634
AvaII	GGWCC	1 cut(s)	970
BalI	TGGCCA	4 cut(s)	289, 2076, 2103, 2130
BanII	GRGCYC	1 cut(s)	1483
BbsI	GAAGAC	3 cut(s)	549, 1457, 1671
BbvI	GCAGC	8 cut(s)	44, 65, 499, 994, 1513, 1582, 1630, 1957
BccI	CCATC	2 cut(s)	707, 1237
BceAI	ACGGC	3 cut(s)	365, 371, 458
BcgI	CGANNNNNNTGC	2 cut(s)	570, 604
BciT130I	CCWGG	3 cut(s)	14, 974, 1646
BciVI	GTATCC	2 cut(s)	1454, 1940
BclI	TGATCA	1 cut(s)	1149
BcnI	CCSGG	2 cut(s)	1413, 1990
BcoDI	GTCTC	2 cut(s)	1088, 1716
BfaI	CTAG	2 cut(s)	236, 1926
BfmI	CTRYAG	3 cut(s)	561, 1527, 1971
BfoI	RGCGCY	1 cut(s)	1071
BfrI	CTTAAG	1 cut(s)	1622
BfuAI	ACCTGC	1 cut(s)	554
BfuI	GTATCC	2 cut(s)	1454, 1940
BglI	GCCNNNNNGGC	1 cut(s)	884
Bme1390I	CCNGG	5 cut(s)	14, 974, 1413, 1646, 1990
Bme18I	GGWCC	1 cut(s)	970
BmeT110I	CYCGRG	1 cut(s)	1634
BmgT120I	GGNCC	3 cut(s)	370, 607, 970
BmiI	GGNNCC	5 cut(s)	379, 551, 609, 1480, 2038
BmrFI	CCNGG	5 cut(s)	14, 974, 1413, 1646, 1990
BmrI	ACTGGG	3 cut(s)	1303, 1818, 1949
BmsI	GCATC	6 cut(s)	92, 1327, 1498, 1726, 1963, 1975
BmuI	ACTGGG	3 cut(s)	1303, 1818, 1949
BpiI	GAAGAC	3 cut(s)	549, 1457, 1671
BpmI	CTGGAG	3 cut(s)	536, 783, 1509
Bpu10I	CCTNAGC	1 cut(s)	1920
Bpu14I	TTCGAA	1 cut(s)	1439
BpuEI	CTTGAG	4 cut(s)	98, 323, 1523, 1785
BpuMI	CCSGG	2 cut(s)	1413, 1990
BsaI	GGTCTC	1 cut(s)	1088
BsaJI	CCNNGG	7 cut(s)	12, 13, 373, 381, 2092, 2119, 2146
BsaXI	ACNNNNNCTCC	2 cut(s)	630, 660
Bsc4I	CCNNNNNNNGG	5 cut(s)	125, 480, 713, 804, 1939
Bse118I	RCCGGY	1 cut(s)	692
Bse1I	ACTGG	8 cut(s)	327, 553, 766, 776, 1216, 1298, 1813, 1944
Bse3DI	GCAATG	6 cut(s)	468, 537, 957, 1283, 1601, 1719
BseBI	CCWGG	3 cut(s)	14, 974, 1646
BseDI	CCNNGG	7 cut(s)	12, 13, 373, 381, 2092, 2119, 2146
BseGI	GGATG	3 cut(s)	1086, 1324, 1954
BseLI	CCNNNNNNNGG	5 cut(s)	125, 480, 713, 804, 1939
BseMI	GCAATG	6 cut(s)	468, 537, 957, 1283, 1601, 1719
BseMII	CTCAG	1 cut(s)	1374
BseNI	ACTGG	8 cut(s)	327, 553, 766, 776, 1216, 1298, 1813, 1944
BseRI	GAGGAG	1 cut(s)	1409
BseXI	GCAGC	8 cut(s)	44, 65, 499, 994, 1513, 1582, 1630, 1957
BseYI	CCCAGC	2 cut(s)	198, 964
BsgI	GTGCAG	2 cut(s)	1099, 2049
Bsh1236I	CGCG	1 cut(s)	995
Bsh1285I	CGRYCG	2 cut(s)	876, 1371
BshFI	GGCC	9 cut(s)	289, 355, 371, 386, 608, 692, 2076, 2103, 2130
BsiEI	CGRYCG	2 cut(s)	876, 1371
BsiHKCI	CYCGRG	1 cut(s)	1634
BsiSI	CCGG	3 cut(s)	693, 1413, 1989
BslFI	GGGAC	3 cut(s)	281, 496, 2005
BslI	CCNNNNNNNGG	5 cut(s)	125, 480, 713, 804, 1939
BsmAI	GTCTC	2 cut(s)	1088, 1716
BsmBI	CGTCTC	1 cut(s)	1716
BsmFI	GGGAC	3 cut(s)	281, 496, 2005
BsnI	GGCC	9 cut(s)	289, 355, 371, 386, 608, 692, 2076, 2103, 2130
Bso31I	GGTCTC	1 cut(s)	1088
BsoBI	CYCGRG	1 cut(s)	1634
Bsp119I	TTCGAA	1 cut(s)	1439
Bsp1286I	GDGCHC	1 cut(s)	1483
Bsp143I	GATC	5 cut(s)	1149, 1191, 1517, 1728, 1787
Bsp19I	CCATGG	3 cut(s)	2092, 2119, 2146
BspACI	CCGC	8 cut(s)	422, 872, 968, 995, 1080, 1130, 1599, 1958
BspANI	GGCC	9 cut(s)	289, 355, 371, 386, 608, 692, 2076, 2103, 2130
BspCNI	CTCAG	1 cut(s)	1375
BspFNI	CGCG	1 cut(s)	995
BspHI	TCATGA	1 cut(s)	445
BspLI	GGNNCC	5 cut(s)	379, 551, 609, 1480, 2038
BspMAI	CTGCAG	3 cut(s)	565, 1531, 1975
BspMI	ACCTGC	1 cut(s)	554
BspPI	GGATC	2 cut(s)	1736, 1795
BspQI	GCTCTTC	2 cut(s)	44, 1867
BspT104I	TTCGAA	1 cut(s)	1439
BspTI	CTTAAG	1 cut(s)	1622
BspTNI	GGTCTC	1 cut(s)	1088
BsrDI	GCAATG	6 cut(s)	468, 537, 957, 1283, 1601, 1719
BsrFI	RCCGGY	1 cut(s)	692
BsrI	ACTGG	8 cut(s)	327, 553, 766, 776, 1216, 1298, 1813, 1944
BssAI	RCCGGY	1 cut(s)	692
BssECI	CCNNGG	7 cut(s)	12, 13, 373, 381, 2092, 2119, 2146
BssMI	GATC	5 cut(s)	1149, 1191, 1517, 1728, 1787
BssT1I	CCWWGG	4 cut(s)	373, 2092, 2119, 2146
Bst2UI	CCWGG	3 cut(s)	14, 974, 1646
Bst4CI	ACNGT	3 cut(s)	166, 802, 1827
Bst6I	CTCTTC	4 cut(s)	44, 894, 1263, 1867
BstAFI	CTTAAG	1 cut(s)	1622
BstAPI	GCANNNNNTGC	1 cut(s)	1979
BstBI	TTCGAA	1 cut(s)	1439
BstC8I	GCNNGC	7 cut(s)	30, 694, 811, 1118, 1629, 2000, 2021
BstDEI	CTNAG	4 cut(s)	625, 1045, 1383, 1920
BstDSI	CCRYGG	4 cut(s)	381, 2092, 2119, 2146
BstENI	CCTNNNNNAGG	1 cut(s)	123
BstF5I	GGATG	3 cut(s)	1086, 1324, 1954
BstFNI	CGCG	1 cut(s)	995
BstH2I	RGCGCY	1 cut(s)	1071
BstHHI	GCGC	3 cut(s)	83, 1070, 1342
BstKTI	GATC	5 cut(s)	1152, 1194, 1520, 1731, 1790
BstMAI	GTCTC	2 cut(s)	1088, 1716
BstMBI	GATC	5 cut(s)	1149, 1191, 1517, 1728, 1787
BstMCI	CGRYCG	2 cut(s)	876, 1371
BstMWI	GCNNNNNNNGC	9 cut(s)	377, 626, 689, 884, 1532, 1624, 1874, 1979, 2190
BstNI	CCWGG	3 cut(s)	14, 974, 1646
BstNSI	RCATGY	3 cut(s)	1019, 1998, 2023
BstSCI	CCNGG	5 cut(s)	12, 972, 1411, 1644, 1988
BstSFI	CTRYAG	3 cut(s)	561, 1527, 1971
BstUI	CGCG	1 cut(s)	995
BstV1I	GCAGC	8 cut(s)	44, 65, 499, 994, 1513, 1582, 1630, 1957
BstV2I	GAAGAC	3 cut(s)	549, 1457, 1671
BstXI	CCANNNNNNTGG	6 cut(s)	363, 2084, 2099, 2111, 2126, 2138
BsuI	GTATCC	2 cut(s)	1454, 1940
BsuRI	GGCC	9 cut(s)	289, 355, 371, 386, 608, 692, 2076, 2103, 2130
BtgI	CCRYGG	4 cut(s)	381, 2092, 2119, 2146
BtsCI	GGATG	3 cut(s)	1086, 1324, 1954
BtsI	GCAGTG	2 cut(s)	588, 1914
BtsIMutI	CAGTG	5 cut(s)	150, 588, 798, 1914, 1937
BveI	ACCTGC	1 cut(s)	554
Cac8I	GCNNGC	7 cut(s)	30, 694, 811, 1118, 1629, 2000, 2021
CaiI	CAGNNNCTG	1 cut(s)	512
CciI	TCATGA	1 cut(s)	445
CfoI	GCGC	3 cut(s)	83, 1070, 1342
Cfr10I	RCCGGY	1 cut(s)	692
Cfr13I	GGNCC	3 cut(s)	370, 607, 970
Csp6I	GTAC	2 cut(s)	343, 1215
CviQI	GTAC	2 cut(s)	343, 1215
DdeI	CTNAG	4 cut(s)	625, 1045, 1383, 1920
DpnI	GATC	5 cut(s)	1151, 1193, 1519, 1730, 1789
DpnII	GATC	5 cut(s)	1149, 1191, 1517, 1728, 1787
DraI	TTTAAA	1 cut(s)	1693
EaeI	YGGCCR	6 cut(s)	287, 353, 384, 2074, 2101, 2128
Eam1104I	CTCTTC	4 cut(s)	44, 894, 1263, 1867
EarI	CTCTTC	4 cut(s)	44, 894, 1263, 1867
Eco130I	CCWWGG	4 cut(s)	373, 2092, 2119, 2146
Eco24I	GRGCYC	1 cut(s)	1483
Eco31I	GGTCTC	1 cut(s)	1088
Eco32I	GATATC	1 cut(s)	1503
Eco47I	GGWCC	1 cut(s)	970
Eco57I	CTGAAG	3 cut(s)	759, 882, 1683
Eco88I	CYCGRG	1 cut(s)	1634
EcoNI	CCTNNNNNAGG	1 cut(s)	123
EcoRII	CCWGG	3 cut(s)	12, 972, 1644
EcoRV	GATATC	1 cut(s)	1503
EcoT14I	CCWWGG	4 cut(s)	373, 2092, 2119, 2146
EcoT38I	GRGCYC	1 cut(s)	1483
ErhI	CCWWGG	4 cut(s)	373, 2092, 2119, 2146
Esp3I	CGTCTC	1 cut(s)	1716
FalI	AAGNNNNNCTT	2 cut(s)	41, 73
FaqI	GGGAC	3 cut(s)	281, 496, 2005
FauNDI	CATATG	1 cut(s)	298
FbaI	TGATCA	1 cut(s)	1149
FokI	GGATG	3 cut(s)	1073, 1311, 1941
FriOI	GRGCYC	1 cut(s)	1483
FspBI	CTAG	2 cut(s)	236, 1926
FspI	TGCGCA	1 cut(s)	82
GlaI	GCGC	3 cut(s)	82, 1069, 1341
GsaI	CCCAGC	2 cut(s)	202, 968
GsuI	CTGGAG	3 cut(s)	536, 783, 1509
HaeII	RGCGCY	1 cut(s)	1071
HaeIII	GGCC	9 cut(s)	289, 355, 371, 386, 608, 692, 2076, 2103, 2130
HapII	CCGG	3 cut(s)	693, 1413, 1989
HhaI	GCGC	3 cut(s)	83, 1070, 1342
Hin6I	GCGC	3 cut(s)	81, 1068, 1340
HinP1I	GCGC	3 cut(s)	81, 1068, 1340
HincII	GTYRAC	1 cut(s)	1348
HindII	GTYRAC	1 cut(s)	1348
HinfI	GANTC	6 cut(s)	767, 794, 933, 1000, 1491, 1677
HpaII	CCGG	3 cut(s)	693, 1413, 1989
HphI	GGTGA	5 cut(s)	526, 1009, 1172, 1241, 1252
Hpy166II	GTNNAC	3 cut(s)	534, 1021, 1348
Hpy188I	TCNGA	7 cut(s)	48, 88, 99, 432, 1149, 1507, 2188
Hpy8I	GTNNAC	3 cut(s)	534, 1021, 1348
Hpy99I	CGWCG	1 cut(s)	595
HpyAV	CCTTC	7 cut(s)	119, 165, 197, 490, 722, 1427, 1694
HpyCH4III	ACNGT	3 cut(s)	166, 802, 1827
HpyCH4IV	ACGT	1 cut(s)	284
HpyF10VI	GCNNNNNNNGC	9 cut(s)	377, 626, 689, 884, 1532, 1624, 1874, 1979, 2190
HpyF3I	CTNAG	4 cut(s)	625, 1045, 1383, 1920
HpySE526I	ACGT	1 cut(s)	284
HspAI	GCGC	3 cut(s)	81, 1068, 1340
KroI	GCCGGC	1 cut(s)	692
KroNI	GCCGGC	1 cut(s)	694
Ksp22I	TGATCA	1 cut(s)	1149
Kzo9I	GATC	5 cut(s)	1149, 1191, 1517, 1728, 1787
LguI	GCTCTTC	2 cut(s)	44, 1867
LmnI	GCTCC	3 cut(s)	377, 555, 1478
Lsp1109I	GCAGC	8 cut(s)	44, 65, 499, 994, 1513, 1582, 1630, 1957
LweI	GCATC	6 cut(s)	92, 1327, 1498, 1726, 1963, 1975
MaeI	CTAG	2 cut(s)	236, 1926
MaeII	ACGT	1 cut(s)	284
MaeIII	GTNAC	4 cut(s)	318, 566, 1105, 1160
MalI	GATC	5 cut(s)	1151, 1193, 1519, 1730, 1789
MboI	GATC	5 cut(s)	1149, 1191, 1517, 1728, 1787
MboII	GAAGA	9 cut(s)	61, 549, 875, 911, 1250, 1368, 1462, 1676, 1884
MfeI	CAATTG	4 cut(s)	303, 486, 1024, 1428
MhlI	GDGCHC	1 cut(s)	1483
MlsI	TGGCCA	4 cut(s)	289, 2076, 2103, 2130
MluNI	TGGCCA	4 cut(s)	289, 2076, 2103, 2130
MlyI	GAGTC	2 cut(s)	788, 1500
MmeI	TCCRAC	3 cut(s)	1448, 1699, 1885
Mox20I	TGGCCA	4 cut(s)	289, 2076, 2103, 2130
MroNI	GCCGGC	1 cut(s)	692
MscI	TGGCCA	4 cut(s)	289, 2076, 2103, 2130
MseI	TTAA	6 cut(s)	213, 687, 825, 1563, 1623, 1692
MslI	CAYNNNNRTG	8 cut(s)	361, 1611, 1712, 2082, 2097, 2109, 2124, 2136
Msp20I	TGGCCA	4 cut(s)	289, 2076, 2103, 2130
MspA1I	CMGCKG	2 cut(s)	968, 1007
MspCI	CTTAAG	1 cut(s)	1622
MspI	CCGG	3 cut(s)	693, 1413, 1989
MspR9I	CCNGG	5 cut(s)	14, 974, 1413, 1646, 1990
MunI	CAATTG	4 cut(s)	303, 486, 1024, 1428
MvaI	CCWGG	3 cut(s)	14, 974, 1646
MvnI	CGCG	1 cut(s)	995
MwoI	GCNNNNNNNGC	9 cut(s)	377, 626, 689, 884, 1532, 1624, 1874, 1979, 2190
NaeI	GCCGGC	1 cut(s)	694
NciI	CCSGG	2 cut(s)	1413, 1990
NcoI	CCATGG	3 cut(s)	2092, 2119, 2146
NdeI	CATATG	1 cut(s)	298
NdeII	GATC	5 cut(s)	1149, 1191, 1517, 1728, 1787
NgoMIV	GCCGGC	1 cut(s)	692
NlaIV	GGNNCC	5 cut(s)	379, 551, 609, 1480, 2038
NmuCI	GTSAC	2 cut(s)	1105, 1160
NsbI	TGCGCA	1 cut(s)	82
NspI	RCATGY	3 cut(s)	1019, 1998, 2023
NspV	TTCGAA	1 cut(s)	1439
PaeI	GCATGC	1 cut(s)	2023
PaeR7I	CTCGAG	1 cut(s)	1634
PagI	TCATGA	1 cut(s)	445
PasI	CCCWGGG	1 cut(s)	13
PciI	ACATGT	1 cut(s)	1015
PciSI	GCTCTTC	2 cut(s)	44, 1867
PdiI	GCCGGC	1 cut(s)	694
PfeI	GAWTC	4 cut(s)	767, 933, 1000, 1677
PflMI	CCANNNNNTGG	1 cut(s)	804
PleI	GAGTC	2 cut(s)	788, 1499
PpsI	GAGTC	2 cut(s)	788, 1499
PscI	ACATGT	1 cut(s)	1015
Psp6I	CCWGG	3 cut(s)	12, 972, 1644
PspFI	CCCAGC	2 cut(s)	198, 964
PspGI	CCWGG	3 cut(s)	12, 972, 1644
PspN4I	GGNNCC	5 cut(s)	379, 551, 609, 1480, 2038
PspPI	GGNCC	3 cut(s)	370, 607, 970
PstI	CTGCAG	3 cut(s)	565, 1531, 1975
PstNI	CAGNNNCTG	1 cut(s)	512
PvuII	CAGCTG	1 cut(s)	1007
RsaI	GTAC	2 cut(s)	344, 1216
RsaNI	GTAC	2 cut(s)	343, 1215
RseI	CAYNNNNRTG	8 cut(s)	361, 1611, 1712, 2082, 2097, 2109, 2124, 2136
SapI	GCTCTTC	2 cut(s)	44, 1867
SaqAI	TTAA	6 cut(s)	213, 687, 825, 1563, 1623, 1692
Sau3AI	GATC	5 cut(s)	1149, 1191, 1517, 1728, 1787
Sau96I	GGNCC	3 cut(s)	370, 607, 970
SchI	GAGTC	2 cut(s)	788, 1500
ScrFI	CCNGG	5 cut(s)	14, 974, 1413, 1646, 1990
SduI	GDGCHC	1 cut(s)	1483
SfaNI	GCATC	6 cut(s)	92, 1327, 1498, 1726, 1963, 1975
SfcI	CTRYAG	3 cut(s)	561, 1527, 1971
Sfr274I	CTCGAG	1 cut(s)	1634
SfuI	TTCGAA	1 cut(s)	1439
SinI	GGWCC	1 cut(s)	970
SlaI	CTCGAG	1 cut(s)	1634
SmiMI	CAYNNNNRTG	8 cut(s)	361, 1611, 1712, 2082, 2097, 2109, 2124, 2136
SmlI	CTYRAG	6 cut(s)	113, 338, 1538, 1622, 1634, 1800
SmoI	CTYRAG	6 cut(s)	113, 338, 1538, 1622, 1634, 1800
SphI	GCATGC	1 cut(s)	2023
SsiI	CCGC	8 cut(s)	422, 872, 968, 995, 1080, 1130, 1599, 1958
SspMI	CTAG	2 cut(s)	236, 1926
StyD4I	CCNGG	5 cut(s)	12, 972, 1411, 1644, 1988
StyI	CCWWGG	4 cut(s)	373, 2092, 2119, 2146
TaaI	ACNGT	3 cut(s)	166, 802, 1827
TaiI	ACGT	1 cut(s)	287
TaqI	TCGA	7 cut(s)	518, 744, 897, 1371, 1439, 1516, 1635
TaqII	GACCGA	1 cut(s)	1357
TauI	GCSGC	3 cut(s)	425, 1133, 1601
TfiI	GAWTC	4 cut(s)	767, 933, 1000, 1677
Tru1I	TTAA	6 cut(s)	213, 687, 825, 1563, 1623, 1692
Tru9I	TTAA	6 cut(s)	213, 687, 825, 1563, 1623, 1692
TscAI	CASTG	5 cut(s)	150, 588, 805, 1921, 1944
TseFI	GTSAC	2 cut(s)	1105, 1160
TseI	GCWGC	8 cut(s)	32, 78, 512, 1007, 1526, 1595, 1618, 1970
Tsp45I	GTSAC	2 cut(s)	1105, 1160
TspDTI	ATGAA	2 cut(s)	434, 925
TspGWI	ACGGA	1 cut(s)	1065
TspRI	CASTG	5 cut(s)	150, 588, 805, 1921, 1944
Van91I	CCANNNNNTGG	1 cut(s)	804
Vha464I	CTTAAG	1 cut(s)	1622
VpaK11BI	GGWCC	1 cut(s)	970
XagI	CCTNNNNNAGG	1 cut(s)	123
XapI	RAATTY	4 cut(s)	433, 1232, 1405, 1688
XceI	RCATGY	3 cut(s)	1019, 1998, 2023
XhoI	CTCGAG	1 cut(s)	1634
XspI	CTAG	2 cut(s)	236, 1926

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.