Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0001793

Physical Location & Seq

Genomic Coordinates Reverse (-)

17727792 .. 17735808

Gene Structure 8017 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G008180.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3168 bp

ATGGCTGGCCAGCAGGACCAAAACAAGGCGGCTAAAAAGTTGCAGAAAAGCTATTTTGATGTGTTGGGTTTGTGCTGTTCATCAGAAGTGCCTTTAGTTGAGAACATCCTGAAGCCTCTTCTTGGTGTCAAAGAGGTTTCTGTGGTTGTGGCTACAAGAACTGTGATTGTTGTTCATGATAGCCTCCTCATTTCTCAGCTTCAAATTGTTAAGGAATTGAACCAAGCAAGACTAGAAGCAAATGTGAGAGTGTATGGGGCAGAGGACAATTTCAAGCAAAAATGGCCAAGTCCATATGCAATTGCTAGTGGCGTCCTTCTCTTGCCTTCATTTCTCAAATACGTGTATCGACCACTGGGATGGCTTGCTCTCTGTGCGGTCGCTGTCGGCATTTTCCCTATTGCTATGAAAGGCATAGCATCCATTCGAAATCTGAGGCTTGACATCAACATCCTTATGATTGTTGCAGTGATTGGTACAATTGCTCTGAATGATTATCTGGAAGCTGGCACTATTGTCTTTCTCTTCACCATCGCAGAGTGGCTTGAGTCAAGGGCAGGGCACAAGGCAAAAGCAGTGATGTCATCGTTGATGAGTATGGCCCCTCAGAAAGCAGTGTTAGCGGATACAGGAGAAGTTGTGGATGTTGATGAGGTTAAGTTAAACACACTTCTTGCTGTGAAGGCCGGTGAAGTTATTCCCATTGATGGAATAGTTGTTGATGGCAAAGGTGAGGTGGATGAGAAAACACTAACAGGAGAGTCATATCCAGTTACCAAGGAAAAGGACTCCACTGTTTGGGCTGGCACCGTCAATCTCAATGGTTTTATTAGTGTGAAAACTACAGCCTTAGCTGAAGACTGTGTGGTTGCTGAAATGTCTAAACTGGTGGAGGAGGCTCAAAACAGCAAAACCCGAACTGAGAGATTCATTGATAAGTGTGCAAAGTTTTATACTCCAGCGGTCCTAGTCATATCGATTTCTATTGCAGCGATACCAGCTGCATTACGTGTTCATAATTGGAGCAAATGGTTTCACTTGGCTTTGGTTGTTTTAGTAAGTGCTTGTCCGTGTGCACTCATCCTCTCTACACCAGTTGTGACTTTCTGCACACTTACAAAAGCAGCAACTTCTGGTCTTTTGATCAAAGGGGGTGACTTCATTGAGATTCTTGCTAAAGTCAAAATCATGGCTTTTGACAAAACTGGTACGATAACAAGGGGTGAATTTGTGGTGATGGATTTTCAATCTCTCCGTGACGACATTAGCTTGAACACTTTGATTTACTGGGTTTCAAGCATTGAGAGGAAGGCTAGTCATCCAATGGCAGCGGCTCTTGTTGATTACGGAAGATCATTTTCAATTGAGCCAAACCCTGAAAATGTGGAGAACTTCGAAAACTTTCCTGGGGAAGGCATCCATGGAAAGATTGATGGACAAGATATTTATATTGGAAGCAAAAGAATAGCTTTGAGAGCGTCTTGTGAAACAATTCCAACCATTGAAGGATCTAAGGGTGGCAAGACTATAGGGTACATATACTGTGAACGAACCCCTGCTGGAGTCTTTACACTGTCTGATGCCTGTCGAACTGGGGCTGCAGATGCTGTTAGGGAGCTGAAGAAATTGGGTATTAAAACAGCCATGCTTACTGGAGATAGCCATGCCGCAGCTACGCAAGCAAATGAGCAGCTTGAGCAAGCTCTGGATGTAGTGCACGCAGAACTTCTTCCTGAAGACAAGGCAAAAATCATTAAAGAATTTAAAATGGAAGGAAACATAGCCATGGTTGGGGATGGCATCAACGATGCCCCTGCTTTGGCCACAGCTGATATTGGTATCTCGATGGGCATTTCAGGGTCAGCCCTTGCTCAAGACACTGGGAATATCATTTTAATGTCAAATGATGTCAGGAAACTACCGAAGGCAATCCAGCTTGCAAGAAGAGCCAAACGAAAAGTGATCGAGAATGTGATCTTATCAATCAGTACTAAAGTTGGTATTCTTGCACTGGCATTTGCAGGACATCCACTTGTTTGGGCAGCAGTTCTTGCAGATGTTGGGACATGCTTGCTTGTGATATTGAACAGCATGCTACTTTTGCAAGGAACTCACAGGCATGGAGAGAAACATGTACATAAGCATGGAAAACATGAACACAGCCACTCTCACAGAAACCAGAAGTGTTTCTCTGACCAAAAAGTTGCAGAAGCGTGCAAGTCTCAAAGATGTGCTTCTCAGAGGTGCGAATCAGAATGCAGGCCTAGTTCTAATTCGAGCTTGCCTGGCAATCATAAATGCAGTAGTGACTTACATGAGGCAAAGCACCATGATCATGGAAGCTGCAGATCTCATAACCATGGAAGCAATAGGCAAAACCACGCTCACACAAACCAGCATCGTTGCTCGGAAAGCAAAACTGTAGTAGCGTGTGAACCTCCAAAGTGTTCTTTTCAGAGTTGTGGATCAGACTGCCAGCCTAGTCCTCTGAAGGCAAGCTTGGCTGACAATTGTAAGTGTGAGGGTTCGGAGGAGATTCACAGTTGCCCAAGAAATGATGACTTGCATGAGGCAAAGAACTGTGATAATCATGATCTGGAGTCACAAATTAATCACAAGCATGGCTGTTTGCAGACTGAAAATTTCAGCTCATGTGAAGATCATGGGTGCACTCACTTGACTGTAAGGCATGGTCATGGTAGTTGTGGAGAGAGTCATGGAATCCATGAGAAAAAAGACTGCAACCACCATTCTGCTGCTTCTTTGGAGGAAAATTGGAGATCAAAGAGTATCGATCATTGCCACTCAAGTCATTGTGACAAAGAACATAGCAGCAATGAAGCTTCTGAACAAATAGCTAGAACAAGCTGTGAGGAGCATCATCATCATCATCAACAACATCAGCATTATCATCATCAAGATCAAAAATCACCTCCTCATATGACTATCGATATTGTGCAGAGTAGTAACCACACAGAATCAGCGTCCCCACATCCTTGCCTCAGCTTAGGAACAAGGGGAAATGAGGTGTGTTGTTCACAACCGATGGCCTGTATGTTGGAAAAAAGAGAATTTGGTGGATGCTGCAAAAGCTACATGAAGGAATGCTGTAGTGGACATGGACCTATTGAATCTAGCTATCAAGTTAGCAAAGTAACAATTGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006829 GO:0006950 GO:0008150 GO:0009506 GO:0009636 GO:0010035 GO:0010038 GO:0010043 GO:0015691 GO:0016020 GO:0030001 GO:0030054 GO:0031090 GO:0032025 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055065 GO:0055069 GO:0055076 GO:0055080 GO:0061687 GO:0065007 GO:0065008 GO:0070838 GO:0071585 GO:0071944 GO:0072507 GO:0072511 GO:0097501 GO:0098588 GO:0098754 GO:0098771 GO:0098805 GO:1990170

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

1055

Amino Acids

115.0

Weight (kDa)

6.57

Isoelectric Point (pI)

41.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	208 - 298	4.3e-21	P-type ATPase actuator domain
Hydrolase	PF00702	394 - 611	9.6e-27	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001793)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19110 AT2G19110 AT2G19110 AT4G30110 AT4G30110 AT4G30120 AT4G30120
fragaria_vesca	FvH4_4g07520 FvH4_4g07520 FvH4_4g07520 FvH4_4g07520 FvH4_6g33520 FvH4_6g33520 FvH4_6g33520 FvH4_6g33520
malus_domestica	MD00G1104400.v1.1 MD01G1183400.v1.1 MD01G1183700.v1.1 MD01G1184000.v1.1 MD01G1184300.v1.1 MD13G1244500.v1.1 MD16G1252700.v1.1
prunus_persica	Prupe.1G078700_v2.0.a1 Prupe.1G078700_v2.0.a1 Prupe.1G078700_v2.0.a1
pyrus_communis	pycom13g21550 pycom16g21330
rosa_chinensis	RchiOBHm_Chr2g0143431 RchiOBHm_Chr4g0400101
rosa_laevigata	RLG00000009214 RLG00000020035
rosa_multiflora	Rmu_sc0000037.1_g000006
rosa_roxburghii	Rroxscaffold_2G00101850 Rroxscaffold_5G00345040 Rroxscaffold_5G00345070
rosa_rugosa	Rorug02G0381400 Rorug04G0022600 Rorug04G0022600
rosa_samantha	Rh2AG434400 Rh2BG442200 Rh2CG420300 Rh2DG452400 Rh4BG096400 Rh4CG107500 Rh4DG093000
rosa_wichuraiana	Rw2G035400 Rw4G008180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	806
AccB7I	CCANNNNNTGG	1 cut(s)	798
AciI	CCGC	6 cut(s)	29, 377, 623, 962, 1331, 1668
AclWI	GGATC	2 cut(s)	1516, 2473
AcoI	YGGCCR	3 cut(s)	7, 284, 1821
AcsI	RAATTY	4 cut(s)	1226, 1760, 2641, 3071
AcuI	CTGAAG	5 cut(s)	131, 876, 1640, 1755, 2510
AcyI	GRCGYC	1 cut(s)	312
AfaI	GTAC	5 cut(s)	478, 1210, 1535, 1990, 2136
AfiI	CCNNNNNNNGG	7 cut(s)	25, 122, 707, 798, 1412, 1791, 1819
AflIII	ACRYGT	3 cut(s)	342, 1009, 2131
AjnI	CCWGG	2 cut(s)	1405, 2284
AjuI	GAANNNNNNNTTGG	2 cut(s)	2483, 2515
Alw21I	GWGCWC	3 cut(s)	1078, 1719, 2672
Alw26I	GTCTC	1 cut(s)	2226
Alw44I	GTGCAC	3 cut(s)	1074, 1715, 2668
AlwI	GGATC	2 cut(s)	1516, 2473
AlwNI	CAGNNNCTG	1 cut(s)	1607
AoxI	GGCC	7 cut(s)	7, 284, 600, 684, 1821, 2261, 3048
ApaLI	GTGCAC	3 cut(s)	1074, 1715, 2668
ApoI	RAATTY	4 cut(s)	1226, 1760, 2641, 3071
AseI	ATTAAT	1 cut(s)	2610
Asp700I	GAANNNNTTC	5 cut(s)	696, 1401, 1491, 1728, 2186
AspS9I	GGNCC	4 cut(s)	16, 601, 964, 3122
AsuHPI	GGTGA	7 cut(s)	520, 701, 743, 1166, 1235, 1246, 2922
AsuII	TTCGAA	2 cut(s)	427, 1395
AvaII	GGWCC	3 cut(s)	16, 964, 3122
BaeGI	GKGCMC	4 cut(s)	564, 1078, 1719, 2672
BalI	TGGCCA	3 cut(s)	9, 286, 1823
BanI	GGYRCC	1 cut(s)	806
BbsI	GAAGAC	2 cut(s)	864, 1743
Bbv12I	GWGCWC	3 cut(s)	1078, 1719, 2672
BbvCI	CCTCAGC	1 cut(s)	3002
BccI	CCATC	9 cut(s)	354, 539, 701, 716, 1231, 1427, 1790, 1840, 3040
BcgI	CGANNNNNNTGC	2 cut(s)	1796, 1830
BciT130I	CCWGG	2 cut(s)	1407, 2286
BciVI	GTATCC	1 cut(s)	619
BclI	TGATCA	2 cut(s)	1143, 2332
BcoDI	GTCTC	1 cut(s)	2226
BfaI	CTAG	8 cut(s)	233, 306, 968, 1314, 2265, 2481, 2859, 3135
BfmI	CTRYAG	6 cut(s)	843, 1527, 1599, 2344, 2421, 3109
BfuI	GTATCC	1 cut(s)	619
BglII	AGATCT	1 cut(s)	2348
BmcAI	AGTACT	1 cut(s)	1990
Bme1390I	CCNGG	2 cut(s)	1407, 2286
Bme18I	GGWCC	3 cut(s)	16, 964, 3122
BmgT120I	GGNCC	4 cut(s)	16, 601, 964, 3122
BmiI	GGNNCC	2 cut(s)	603, 808
BmrFI	CCNGG	2 cut(s)	1407, 2286
BmrI	ACTGGG	4 cut(s)	365, 1297, 1602, 1890
BmsI	GCATC	9 cut(s)	428, 1425, 1570, 1594, 1798, 1809, 2407, 2887, 3071
BmuI	ACTGGG	4 cut(s)	365, 1297, 1602, 1890
BpiI	GAAGAC	2 cut(s)	864, 1743
BpmI	CTGGAG	4 cut(s)	942, 1581, 1674, 2618
Bpu10I	CCTNAGC	3 cut(s)	850, 3002, 3007
Bpu14I	TTCGAA	2 cut(s)	427, 1395
BpuEI	CTTGAG	4 cut(s)	566, 1715, 1857, 2791
Bsa29I	ATCGAT	3 cut(s)	977, 2793, 2949
BsaAI	YACGTR	2 cut(s)	343, 1010
BsaHI	GRCGYC	1 cut(s)	312
BsaJI	CCNNGG	5 cut(s)	777, 1406, 1420, 1785, 2359
BsaXI	ACNNNNNCTCC	2 cut(s)	624, 654
Bsc4I	CCNNNNNNNGG	7 cut(s)	25, 122, 707, 798, 1412, 1791, 1819
Bse118I	RCCGGY	1 cut(s)	686
Bse3DI	GCAATG	2 cut(s)	2797, 2842
BseBI	CCWGG	2 cut(s)	1407, 2286
BseCI	ATCGAT	3 cut(s)	977, 2793, 2949
BseDI	CCNNGG	5 cut(s)	777, 1406, 1420, 1785, 2359
BseLI	CCNNNNNNNGG	7 cut(s)	25, 122, 707, 798, 1412, 1791, 1819
BseMI	GCAATG	2 cut(s)	2797, 2842
BseMII	CTCAG	6 cut(s)	209, 425, 620, 912, 2252, 3016
BseRI	GAGGAG	5 cut(s)	176, 908, 2546, 2888, 2925
BseSI	GKGCMC	4 cut(s)	564, 1078, 1719, 2672
BsgI	GTGCAG	2 cut(s)	1093, 2978
Bsh1285I	CGRYCG	1 cut(s)	381
BshFI	GGCC	7 cut(s)	9, 286, 602, 686, 1823, 2263, 3050
BshNI	GGYRCC	1 cut(s)	806
BshVI	ATCGAT	3 cut(s)	977, 2793, 2949
BsiEI	CGRYCG	1 cut(s)	381
BsiHKAI	GWGCWC	3 cut(s)	1078, 1719, 2672
BsiSI	CCGG	1 cut(s)	687
BslFI	GGGAC	2 cut(s)	2077, 2971
BslI	CCNNNNNNNGG	7 cut(s)	25, 122, 707, 798, 1412, 1791, 1819
BsmAI	GTCTC	1 cut(s)	2226
BsmFI	GGGAC	2 cut(s)	2077, 2971
BsmI	GAATGC	2 cut(s)	2261, 3110
BsnI	GGCC	7 cut(s)	9, 286, 602, 686, 1823, 2263, 3050
Bsp119I	TTCGAA	2 cut(s)	427, 1395
Bsp1286I	GDGCHC	4 cut(s)	564, 1078, 1719, 2672
Bsp1407I	TGTACA	1 cut(s)	2134
Bsp19I	CCATGG	3 cut(s)	1420, 1785, 2359
BspACI	CCGC	6 cut(s)	29, 377, 623, 962, 1331, 1668
BspANI	GGCC	7 cut(s)	9, 286, 602, 686, 1823, 2263, 3050
BspCNI	CTCAG	6 cut(s)	208, 426, 619, 913, 2251, 3015
BspDI	ATCGAT	3 cut(s)	977, 2793, 2949
BspHI	TCATGA	2 cut(s)	175, 2590
BspLI	GGNNCC	2 cut(s)	603, 808
BspMAI	CTGCAG	2 cut(s)	1603, 2348
BspPI	GGATC	2 cut(s)	1516, 2473
BspQI	GCTCTTC	1 cut(s)	1939
BspT104I	TTCGAA	2 cut(s)	427, 1395
BspT107I	GGYRCC	1 cut(s)	806
BsrDI	GCAATG	2 cut(s)	2797, 2842
BsrFI	RCCGGY	1 cut(s)	686
BsrGI	TGTACA	1 cut(s)	2134
BssAI	RCCGGY	1 cut(s)	686
BssECI	CCNNGG	5 cut(s)	777, 1406, 1420, 1785, 2359
BssNI	GRCGYC	1 cut(s)	312
BssT1I	CCWWGG	4 cut(s)	777, 1420, 1785, 2359
Bst2UI	CCWGG	2 cut(s)	1407, 2286
Bst6I	CTCTTC	3 cut(s)	123, 530, 1939
BstACI	GRCGYC	1 cut(s)	312
BstAPI	GCANNNNNTGC	1 cut(s)	2051
BstAUI	TGTACA	1 cut(s)	2134
BstBAI	YACGTR	2 cut(s)	343, 1010
BstBI	TTCGAA	2 cut(s)	427, 1395
BstDEI	CTNAG	9 cut(s)	195, 434, 606, 850, 921, 1512, 2238, 3002, 3007
BstDSI	CCRYGG	3 cut(s)	1420, 1785, 2359
BstENI	CCTNNNNNAGG	1 cut(s)	1410
BstMAI	GTCTC	1 cut(s)	2226
BstMCI	CGRYCG	1 cut(s)	381
BstNI	CCWGG	2 cut(s)	1407, 2286
BstNSI	RCATGY	3 cut(s)	2070, 2095, 2135
BstSCI	CCNGG	2 cut(s)	1405, 2284
BstSFI	CTRYAG	6 cut(s)	843, 1527, 1599, 2344, 2421, 3109
BstSLI	GKGCMC	4 cut(s)	564, 1078, 1719, 2672
BstV2I	GAAGAC	2 cut(s)	864, 1743
BstX2I	RGATCY	2 cut(s)	1508, 2348
BstXI	CCANNNNNNTGG	3 cut(s)	360, 2037, 2336
BstYI	RGATCY	2 cut(s)	1508, 2348
Bsu15I	ATCGAT	3 cut(s)	977, 2793, 2949
BsuI	GTATCC	1 cut(s)	619
BsuRI	GGCC	7 cut(s)	9, 286, 602, 686, 1823, 2263, 3050
BsuTUI	ATCGAT	3 cut(s)	977, 2793, 2949
BtgI	CCRYGG	3 cut(s)	1420, 1785, 2359
BtgZI	GCGATG	1 cut(s)	517
BtsI	GCAGTG	3 cut(s)	474, 582, 621
BtsIMutI	CAGTG	8 cut(s)	353, 474, 582, 621, 792, 1571, 1878, 2009
CaiI	CAGNNNCTG	1 cut(s)	1607
CciI	TCATGA	2 cut(s)	175, 2590
Cfr10I	RCCGGY	1 cut(s)	686
Cfr13I	GGNCC	4 cut(s)	16, 601, 964, 3122
ClaI	ATCGAT	3 cut(s)	977, 2793, 2949
CseI	GACGC	3 cut(s)	301, 1467, 2973
Csp6I	GTAC	5 cut(s)	477, 1209, 1534, 1989, 2135
CviQI	GTAC	5 cut(s)	477, 1209, 1534, 1989, 2135
DdeI	CTNAG	9 cut(s)	195, 434, 606, 850, 921, 1512, 2238, 3002, 3007
DraI	TTTAAA	1 cut(s)	1765
EaeI	YGGCCR	3 cut(s)	7, 284, 1821
Eam1104I	CTCTTC	3 cut(s)	123, 530, 1939
EarI	CTCTTC	3 cut(s)	123, 530, 1939
Eco130I	CCWWGG	4 cut(s)	777, 1420, 1785, 2359
Eco147I	AGGCCT	1 cut(s)	2263
Eco47I	GGWCC	3 cut(s)	16, 964, 3122
Eco57I	CTGAAG	5 cut(s)	131, 876, 1640, 1755, 2510
EcoNI	CCTNNNNNAGG	1 cut(s)	1410
EcoRII	CCWGG	2 cut(s)	1405, 2284
EcoT14I	CCWWGG	4 cut(s)	777, 1420, 1785, 2359
ErhI	CCWWGG	4 cut(s)	777, 1420, 1785, 2359
FalI	AAGNNNNNCTT	6 cut(s)	1453, 1485, 2218, 2250, 2483, 2515
FaqI	GGGAC	2 cut(s)	2077, 2971
FauNDI	CATATG	2 cut(s)	295, 2940
FbaI	TGATCA	2 cut(s)	1143, 2332
FspBI	CTAG	8 cut(s)	233, 306, 968, 1314, 2265, 2481, 2859, 3135
GsuI	CTGGAG	4 cut(s)	942, 1581, 1674, 2618
HaeIII	GGCC	7 cut(s)	9, 286, 602, 686, 1823, 2263, 3050
HapII	CCGG	1 cut(s)	687
HgaI	GACGC	3 cut(s)	301, 1467, 2973
Hin1I	GRCGYC	1 cut(s)	312
HindIII	AAGCTT	2 cut(s)	2497, 2841
HpaII	CCGG	1 cut(s)	687
HphI	GGTGA	7 cut(s)	520, 701, 743, 1166, 1235, 1246, 2922
Hpy166II	GTNNAC	7 cut(s)	1076, 1547, 1717, 2435, 2670, 3038, 3116
Hpy8I	GTNNAC	7 cut(s)	1076, 1547, 1717, 2435, 2670, 3038, 3116
HpyCH4IV	ACGT	2 cut(s)	342, 1009
HpyF3I	CTNAG	9 cut(s)	195, 434, 606, 850, 921, 1512, 2238, 3002, 3007
HpySE526I	ACGT	2 cut(s)	342, 1009
Hsp92I	GRCGYC	1 cut(s)	312
Ksp22I	TGATCA	2 cut(s)	1143, 2332
LguI	GCTCTTC	1 cut(s)	1939
LmnI	GCTCC	3 cut(s)	1023, 1615, 2875
LweI	GCATC	9 cut(s)	428, 1425, 1570, 1594, 1798, 1809, 2407, 2887, 3071
MaeI	CTAG	8 cut(s)	233, 306, 968, 1314, 2265, 2481, 2859, 3135
MaeII	ACGT	2 cut(s)	342, 1009
MaeIII	GTNAC	9 cut(s)	772, 1099, 1154, 1256, 2306, 2601, 2816, 2966, 3154
MboII	GAAGA	9 cut(s)	110, 517, 869, 1362, 1633, 1721, 1748, 1956, 2669
MfeI	CAATTG	5 cut(s)	300, 480, 1362, 2509, 3159
MflI	RGATCY	2 cut(s)	1508, 2348
MhlI	GDGCHC	4 cut(s)	564, 1078, 1719, 2672
MlsI	TGGCCA	3 cut(s)	9, 286, 1823
MluNI	TGGCCA	3 cut(s)	9, 286, 1823
MlyI	GAGTC	6 cut(s)	557, 770, 782, 1572, 2609, 2722
MmeI	TCCRAC	2 cut(s)	1520, 3039
Mox20I	TGGCCA	3 cut(s)	9, 286, 1823
MroXI	GAANNNNTTC	5 cut(s)	696, 1401, 1491, 1728, 2186
MscI	TGGCCA	3 cut(s)	9, 286, 1823
MseI	TTAA	8 cut(s)	210, 657, 662, 1635, 1755, 1764, 1895, 2610
Msp20I	TGGCCA	3 cut(s)	9, 286, 1823
MspA1I	CMGCKG	4 cut(s)	962, 1001, 1331, 1829
MspI	CCGG	1 cut(s)	687
MspR9I	CCNGG	2 cut(s)	1407, 2286
MunI	CAATTG	5 cut(s)	300, 480, 1362, 2509, 3159
Mva1269I	GAATGC	2 cut(s)	2261, 3110
MvaI	CCWGG	2 cut(s)	1407, 2286
NcoI	CCATGG	3 cut(s)	1420, 1785, 2359
NdeI	CATATG	2 cut(s)	295, 2940
NlaIV	GGNNCC	2 cut(s)	603, 808
NmuCI	GTSAC	6 cut(s)	1099, 1154, 1256, 2306, 2601, 2816
NspI	RCATGY	3 cut(s)	2070, 2095, 2135
NspV	TTCGAA	2 cut(s)	427, 1395
PaeI	GCATGC	1 cut(s)	2095
PagI	TCATGA	2 cut(s)	175, 2590
PceI	AGGCCT	1 cut(s)	2263
PciI	ACATGT	1 cut(s)	2131
PciSI	GCTCTTC	1 cut(s)	1939
PctI	GAATGC	2 cut(s)	2261, 3110
PdmI	GAANNNNTTC	5 cut(s)	696, 1401, 1491, 1728, 2186
PfeI	GAWTC	7 cut(s)	927, 1168, 2249, 2536, 2721, 2978, 3131
PflMI	CCANNNNNTGG	1 cut(s)	798
PleI	GAGTC	6 cut(s)	556, 769, 782, 1571, 2608, 2721
PpsI	GAGTC	6 cut(s)	556, 769, 782, 1571, 2608, 2721
Ppu21I	YACGTR	2 cut(s)	343, 1010
PscI	ACATGT	1 cut(s)	2131
PshBI	ATTAAT	1 cut(s)	2610
Psp6I	CCWGG	2 cut(s)	1405, 2284
PspGI	CCWGG	2 cut(s)	1405, 2284
PspN4I	GGNNCC	2 cut(s)	603, 808
PspPI	GGNCC	4 cut(s)	16, 601, 964, 3122
PstI	CTGCAG	2 cut(s)	1603, 2348
PstNI	CAGNNNCTG	1 cut(s)	1607
PsuI	RGATCY	2 cut(s)	1508, 2348
PvuII	CAGCTG	2 cut(s)	1001, 1829
RsaI	GTAC	5 cut(s)	478, 1210, 1535, 1990, 2136
RsaNI	GTAC	5 cut(s)	477, 1209, 1534, 1989, 2135
SapI	GCTCTTC	1 cut(s)	1939
SaqAI	TTAA	8 cut(s)	210, 657, 662, 1635, 1755, 1764, 1895, 2610
Sau96I	GGNCC	4 cut(s)	16, 601, 964, 3122
ScaI	AGTACT	1 cut(s)	1990
SchI	GAGTC	6 cut(s)	557, 770, 782, 1572, 2609, 2722
ScrFI	CCNGG	2 cut(s)	1407, 2286
SduI	GDGCHC	4 cut(s)	564, 1078, 1719, 2672
SfaNI	GCATC	9 cut(s)	428, 1425, 1570, 1594, 1798, 1809, 2407, 2887, 3071
SfcI	CTRYAG	6 cut(s)	843, 1527, 1599, 2344, 2421, 3109
SfuI	TTCGAA	2 cut(s)	427, 1395
SinI	GGWCC	3 cut(s)	16, 964, 3122
SmlI	CTYRAG	4 cut(s)	545, 1694, 1872, 2806
SmoI	CTYRAG	4 cut(s)	545, 1694, 1872, 2806
SphI	GCATGC	1 cut(s)	2095
SseBI	AGGCCT	1 cut(s)	2263
SsiI	CCGC	6 cut(s)	29, 377, 623, 962, 1331, 1668
SspMI	CTAG	8 cut(s)	233, 306, 968, 1314, 2265, 2481, 2859, 3135
StuI	AGGCCT	1 cut(s)	2263
StyD4I	CCNGG	2 cut(s)	1405, 2284
StyI	CCWWGG	4 cut(s)	777, 1420, 1785, 2359
TaiI	ACGT	2 cut(s)	345, 1012
TatI	WGTACW	2 cut(s)	1988, 2134
TauI	GCSGC	3 cut(s)	32, 1334, 1670
TfiI	GAWTC	7 cut(s)	927, 1168, 2249, 2536, 2721, 2978, 3131
Tru1I	TTAA	8 cut(s)	210, 657, 662, 1635, 1755, 1764, 1895, 2610
Tru9I	TTAA	8 cut(s)	210, 657, 662, 1635, 1755, 1764, 1895, 2610
TscAI	CASTG	8 cut(s)	360, 474, 582, 621, 799, 1578, 1885, 2016
TseFI	GTSAC	6 cut(s)	1099, 1154, 1256, 2306, 2601, 2816
Tsp45I	GTSAC	6 cut(s)	1099, 1154, 1256, 2306, 2601, 2816
TspGWI	ACGGA	3 cut(s)	1059, 1244, 1362
TspRI	CASTG	8 cut(s)	360, 474, 582, 621, 799, 1578, 1885, 2016
Van91I	CCANNNNNTGG	1 cut(s)	798
VneI	GTGCAC	3 cut(s)	1074, 1715, 2668
VpaK11BI	GGWCC	3 cut(s)	16, 964, 3122
VspI	ATTAAT	1 cut(s)	2610
XagI	CCTNNNNNAGG	1 cut(s)	1410
XapI	RAATTY	4 cut(s)	1226, 1760, 2641, 3071
XceI	RCATGY	3 cut(s)	2070, 2095, 2135
XmnI	GAANNNNTTC	5 cut(s)	696, 1401, 1491, 1728, 2186
XspI	CTAG	8 cut(s)	233, 306, 968, 1314, 2265, 2481, 2859, 3135
ZrmI	AGTACT	1 cut(s)	1990

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.