Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0001793

Physical Location & Seq

Genomic Coordinates Reverse (-)

25549364 .. 25559561

Gene Structure 10198 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1244500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2631 bp

ATGGCTTCTGCCCAGGGCAAAGAAAAGCCAGCAGCCATAAAGTTTCAGAAGAGCTACTTTGATGTGTTGGGGTTGTGCTGCTCATCAGAAGTTCCTCTGATAGAGAATATCCTCAAGCCTCTTGCAGGTGTGAAAGAGGTTTCAGTGATTGTGCCTTCCAGAACTGTCATTGTTGTCCATGATACCCTTCTCATTTCTCAGCTTCAAATCGTTAAGGCACTAAACCAAGGAAGGCTAGAAGCAAATGTGAGAGTGTATGGGGCAGGGGACAGTTACAAGAAAACGTGGCCAAGTCCATATGCAATTGGTAGTGGCGTGTTACTGGTTCTATCATTTCTCAAGTATGTATATCGGCCATTAGGATGGTTGGCTCTTGGAGCGGTTGCCGTTGGCATTTTCCCCATTGCTTCGAAAGGTTTTGTGTCTATTCGGAATTTCCGGTTTCATGACATCAACATTCTTCTTATCATTGCCGTAATTGGGACAATCGCTTTGAAGGACTATACAGAAGCTGCCTCGATTGTCTTTCTGTTCACCATTGCAGAGTGGCTCCGGTCTTCTGCAGGTTACAGGGCGAAAGCAGTGATGTCGTCGTTGATGAGCATGGCGCCCCAAAAAGCAGTCTTAGCAGAGAGTGGAGAAGTTGTGGGAGTTGATGAGGTTAAGTTGAACACAATTCTTGCTGTTAAGGCCGGCGAAATTATACCGATTGATGGAATAGTTGTTGAAGGAAAAGCCGAAGTGGATGAGAAAACACTAACTGGAGAATCCTATCCAGTTGCCAAAGAAAAGGACTCCACTATTTGGGCTGGCACTATCAATCTTAATGGTTATATTTGTGTGAAAACTACTTCATTGGCTGAAGATTGTGCGGTTGCCAAAATGGCAAAACTTGTCGAAGAGGCTCAAAACAGCAAAACCAGAACTCAAAGTTTCATTGACAAATGTGCAAGGTTCTATACCCCAGCGGTCCTAGTCATATCTGTTTCTATCGCGGTGATTCCAGCTGTATTACATGTTCACAATTGGAGCAACTGGTTTCACTTAGCTTTGGTTGTTTTGGTGAGCGCATGTCCGTGCGGTCTCATCCTCTCTACACCCGTTGTGACTTTCTGCACGCTCACAAAAGCGGCAACATCTGGACTTCTGATCAAAGGAGGTGACTACATTGAGATTCTTGCTAAAGTCAAGACCATGGCTTTTGACAAAACTGGTACAATAACAAGGGGTGAATTTGTGGTGATGGATTTTCAATCTCTTCGTGATGACATTAGCTTGAACACATTGCTTTACTGGGTTTCAAGCATTGAGAGGAAGTCAAGTCATCCAATGGCAGATGCGCTGGTTGACTATGGAAGATCGCTTTCGGTTGAGCCAAAACCTGAAAATGTGGAGGAGTTTCAAAATTTTCCCGGGGAAGGTATTCATGGGAAAATTGATGGACAAGATATCTACATTGGTAACAGAAAAATTGCTTTGAGAGCTGCTTGTGAAATAGTTCCAACAATTGAAGGTTCTAAAGGTGGGAAGACGATTGGATACATATACTCTGGGGGAACCCCTGCTGGAGTCTTTACAATAGCTGATGCTTGTCGATCTGGGGCTGCAGAGGCTGTCAAGGAGCTAAAGAAGTTAGGCATTAAAACAGCTATGCTCACAGGAGATAGTAATGCTGCCGCATTACATACAAATGAGCAGCTTAAGCGAGCTCTCGAGGTAGTCCATGCAGAACTTCTACCTGAAGACAAGGCAAGAATCATTAAAGAATTTAAAATGGAAGGAAATACAGCAATGGTTGGAGACGGTATCAATGATGCCCCTGCTTTAGCTACAGCCGATATTGGTATCTCAATGGGCATTTCAGGATCAGCCCTTGCTCAAGAAACTGGGAATATAATTTTACTGTCAAATGACATTAGGAAACTACCGAAAGCAGTCCAACTTGCGAGAAGAGCTAATAGAAAAGTGATTGAGAATGTGGTTTTGTCAATCACTACGAAGGTCAGTATCCTTGCACTGGGATTTGCAGGGCATCCGCTCGTTTGGGCTGCAGTTCTTGCTGATGTCGGGACATGCATGCTTGTGATATTCAACAGCATGCTACTCTTACAAGGAACCCATAAGCATGGAGGGAAAACTTCTGCACCACATGGCCATAAACATGGAAGCCATGGACATAGTCATTCTCACAAAAACCAACATTGTTGCTCGGACAGCAAACGCTTAAAAGCAGCCTGTAAACCCCAAAAATGTTCCTCGCAGAAATGTGGATCAGAGTGCCAGCCTAGTCCCTTAAATTCAAGCTTTTCCTATAAGCTTAAGTGCAGTGACTTACATGAGGCACGACACTGTGATGAAACAAGCTGCACCAAAGTCAATCATGATCTCGAGTCGCAGAATAAACACAATCATGGTTGTTTGAGTCATCATAATTTGAGCTCATGCGCAGAAGGTGATAAACTTTACGAGGCAAAACACTGCAATCATTCTGCTTCTTCTTTGGTGGAAATTAAAAAGTTGACAAGTAAAGGTCATTGCCACTCGACCCACTGCGGCAAAGAGCACAACAGAAAAGAAGATGATGGACGAAAATTTGACAAGCGGTGTTTATTGCCACTCAAGCCACAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006829 GO:0006950 GO:0008150 GO:0009506 GO:0009636 GO:0010035 GO:0010038 GO:0010043 GO:0015691 GO:0016020 GO:0030001 GO:0030054 GO:0031090 GO:0032025 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055065 GO:0055069 GO:0055076 GO:0055080 GO:0061687 GO:0065007 GO:0065008 GO:0070838 GO:0071585 GO:0071944 GO:0072507 GO:0072511 GO:0097501 GO:0098588 GO:0098754 GO:0098771 GO:0098805 GO:1990170

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

877

Amino Acids

94.55

Weight (kDa)

8.75

Isoelectric Point (pI)

34.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	205 - 300	1.1e-21	P-type ATPase actuator domain
Hydrolase	PF00702	396 - 613	2e-28	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001793)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19110 AT2G19110 AT2G19110 AT4G30110 AT4G30110 AT4G30120 AT4G30120
fragaria_vesca	FvH4_4g07520 FvH4_4g07520 FvH4_4g07520 FvH4_4g07520 FvH4_6g33520 FvH4_6g33520 FvH4_6g33520 FvH4_6g33520
malus_domestica	MD00G1104400.v1.1 MD01G1183400.v1.1 MD01G1183700.v1.1 MD01G1184000.v1.1 MD01G1184300.v1.1 MD13G1244500.v1.1 MD16G1252700.v1.1
prunus_persica	Prupe.1G078700_v2.0.a1 Prupe.1G078700_v2.0.a1 Prupe.1G078700_v2.0.a1
pyrus_communis	pycom13g21550 pycom16g21330
rosa_chinensis	RchiOBHm_Chr2g0143431 RchiOBHm_Chr4g0400101
rosa_laevigata	RLG00000009214 RLG00000020035
rosa_multiflora	Rmu_sc0000037.1_g000006
rosa_roxburghii	Rroxscaffold_2G00101850 Rroxscaffold_5G00345040 Rroxscaffold_5G00345070
rosa_rugosa	Rorug02G0381400 Rorug04G0022600 Rorug04G0022600
rosa_samantha	Rh2AG434400 Rh2BG442200 Rh2CG420300 Rh2DG452400 Rh4BG096400 Rh4CG107500 Rh4DG093000
rosa_wichuraiana	Rw2G035400 Rw4G008180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	116
Acc16I	TGCGCA	1 cut(s)	2446
Acc36I	ACCTGC	2 cut(s)	116, 554
AccB1I	GGYRCC	1 cut(s)	607
AccB7I	CCANNNNNTGG	1 cut(s)	804
AccBSI	CCGCTC	2 cut(s)	380, 2038
AccII	CGCG	1 cut(s)	995
AclWI	GGATC	2 cut(s)	1873, 2278
AcoI	YGGCCR	3 cut(s)	287, 353, 2152
AcsI	RAATTY	6 cut(s)	433, 1232, 1405, 1766, 2296, 2591
AcuI	CTGAAG	2 cut(s)	882, 1761
AcyI	GRCGYC	1 cut(s)	608
AfaI	GTAC	1 cut(s)	1216
AfiI	CCNNNNNNNGG	6 cut(s)	125, 480, 713, 804, 1418, 2017
AflII	CTTAAG	2 cut(s)	1700, 2318
AflIII	ACRYGT	1 cut(s)	1015
AjnI	CCWGG	1 cut(s)	12
Alw21I	GWGCWC	3 cut(s)	1711, 2441, 2565
Alw26I	GTCTC	2 cut(s)	1088, 1794
AlwI	GGATC	2 cut(s)	1873, 2278
AlwNI	CAGNNNCTG	2 cut(s)	512, 1613
Ama87I	CYCGRG	3 cut(s)	1412, 1712, 2387
AoxI	GGCC	4 cut(s)	287, 353, 690, 2152
ApoI	RAATTY	6 cut(s)	433, 1232, 1405, 1766, 2296, 2591
ArsI	GACNNNNNNTTYG	2 cut(s)	475, 507
Asp700I	GAANNNNTTC	2 cut(s)	1422, 1497
AspLEI	GCGC	4 cut(s)	610, 1070, 1342, 2447
AspS9I	GGNCC	1 cut(s)	970
AsuC2I	CCSGG	2 cut(s)	1413, 1414
AsuHPI	GGTGA	7 cut(s)	526, 1009, 1075, 1172, 1241, 1252, 2465
AsuII	TTCGAA	1 cut(s)	410
AvaI	CYCGRG	3 cut(s)	1412, 1712, 2387
AvaII	GGWCC	1 cut(s)	970
BalI	TGGCCA	2 cut(s)	289, 2154
BanI	GGYRCC	1 cut(s)	607
BanII	GRGCYC	2 cut(s)	1711, 2441
BbsI	GAAGAC	3 cut(s)	549, 1535, 1749
Bbv12I	GWGCWC	3 cut(s)	1711, 2441, 2565
BccI	CCATC	5 cut(s)	357, 707, 1237, 1433, 2576
BceAI	ACGGC	2 cut(s)	371, 458
BcgI	CGANNNNNNTGC	2 cut(s)	570, 604
BciT130I	CCWGG	1 cut(s)	14
BciVI	GTATCC	2 cut(s)	1532, 2018
BclI	TGATCA	1 cut(s)	1149
BcnI	CCSGG	2 cut(s)	1413, 1414
BcoDI	GTCTC	2 cut(s)	1088, 1794
BfaI	CTAG	3 cut(s)	236, 974, 2286
BfmI	CTRYAG	4 cut(s)	561, 1605, 1830, 2049
BfoI	RGCGCY	1 cut(s)	611
BfrI	CTTAAG	2 cut(s)	1700, 2318
BfuAI	ACCTGC	2 cut(s)	116, 554
BfuI	GTATCC	2 cut(s)	1532, 2018
BglI	GCCNNNNNGGC	1 cut(s)	884
Bme1390I	CCNGG	3 cut(s)	14, 1413, 1414
Bme18I	GGWCC	1 cut(s)	970
BmeT110I	CYCGRG	3 cut(s)	1412, 1712, 2387
BmgT120I	GGNCC	1 cut(s)	970
BmiI	GGNNCC	4 cut(s)	551, 609, 1558, 2116
BmrFI	CCNGG	3 cut(s)	14, 1413, 1414
BmrI	ACTGGG	3 cut(s)	1303, 1896, 2027
BmsI	GCATC	4 cut(s)	1327, 1576, 1804, 2041
BmuI	ACTGGG	3 cut(s)	1303, 1896, 2027
BpiI	GAAGAC	3 cut(s)	549, 1535, 1749
BpmI	CTGGAG	2 cut(s)	783, 1587
Bpu14I	TTCGAA	1 cut(s)	410
BpuEI	CTTGAG	4 cut(s)	98, 323, 1863, 2603
BpuMI	CCSGG	2 cut(s)	1413, 1414
BsaHI	GRCGYC	1 cut(s)	608
BsaI	GGTCTC	1 cut(s)	1088
BsaJI	CCNNGG	7 cut(s)	12, 13, 226, 1194, 1412, 1413, 2170
BsaWI	WCCGGW	2 cut(s)	438, 552
BsaXI	ACNNNNNCTCC	2 cut(s)	630, 660
Bsc4I	CCNNNNNNNGG	6 cut(s)	125, 480, 713, 804, 1418, 2017
Bse118I	RCCGGY	1 cut(s)	692
Bse1I	ACTGG	8 cut(s)	327, 766, 776, 1040, 1216, 1298, 1891, 2022
Bse3DI	GCAATG	6 cut(s)	402, 468, 537, 1283, 1797, 2533
BseBI	CCWGG	1 cut(s)	14
BseDI	CCNNGG	7 cut(s)	12, 13, 226, 1194, 1412, 1413, 2170
BseGI	GGATG	5 cut(s)	368, 751, 1086, 1324, 2032
BseLI	CCNNNNNNNGG	6 cut(s)	125, 480, 713, 804, 1418, 2017
BseMI	GCAATG	6 cut(s)	402, 468, 537, 1283, 1797, 2533
BseMII	CTCAG	1 cut(s)	212
BseNI	ACTGG	8 cut(s)	327, 766, 776, 1040, 1216, 1298, 1891, 2022
BseRI	GAGGAG	1 cut(s)	1409
BseYI	CCCAGC	1 cut(s)	964
BsgI	GTGCAG	4 cut(s)	1099, 2127, 2344, 2350
Bsh1236I	CGCG	1 cut(s)	995
BshFI	GGCC	4 cut(s)	289, 355, 692, 2154
BshNI	GGYRCC	1 cut(s)	607
BsiHKAI	GWGCWC	3 cut(s)	1711, 2441, 2565
BsiHKCI	CYCGRG	3 cut(s)	1412, 1712, 2387
BsiSI	CCGG	4 cut(s)	439, 553, 693, 1413
BslFI	GGGAC	4 cut(s)	281, 496, 2083, 2274
BslI	CCNNNNNNNGG	6 cut(s)	125, 480, 713, 804, 1418, 2017
BsmAI	GTCTC	2 cut(s)	1088, 1794
BsmBI	CGTCTC	1 cut(s)	1794
BsmFI	GGGAC	4 cut(s)	281, 496, 2083, 2274
BsnI	GGCC	4 cut(s)	289, 355, 692, 2154
Bso31I	GGTCTC	1 cut(s)	1088
BsoBI	CYCGRG	3 cut(s)	1412, 1712, 2387
Bsp119I	TTCGAA	1 cut(s)	410
Bsp1286I	GDGCHC	3 cut(s)	1711, 2441, 2565
Bsp143I	GATC	6 cut(s)	1149, 1358, 1595, 1865, 2270, 2383
Bsp19I	CCATGG	2 cut(s)	1194, 2170
BspANI	GGCC	4 cut(s)	289, 355, 692, 2154
BspCNI	CTCAG	1 cut(s)	211
BspFNI	CGCG	1 cut(s)	995
BspHI	TCATGA	2 cut(s)	445, 2380
BspLI	GGNNCC	4 cut(s)	551, 609, 1558, 2116
BspMAI	CTGCAG	3 cut(s)	565, 1609, 2053
BspMI	ACCTGC	2 cut(s)	116, 554
BspPI	GGATC	2 cut(s)	1873, 2278
BspQI	GCTCTTC	2 cut(s)	44, 1945
BspT104I	TTCGAA	1 cut(s)	410
BspT107I	GGYRCC	1 cut(s)	607
BspTI	CTTAAG	2 cut(s)	1700, 2318
BspTNI	GGTCTC	1 cut(s)	1088
BsrBI	CCGCTC	2 cut(s)	380, 2038
BsrDI	GCAATG	6 cut(s)	402, 468, 537, 1283, 1797, 2533
BsrFI	RCCGGY	1 cut(s)	692
BsrI	ACTGG	8 cut(s)	327, 766, 776, 1040, 1216, 1298, 1891, 2022
BssAI	RCCGGY	1 cut(s)	692
BssECI	CCNNGG	7 cut(s)	12, 13, 226, 1194, 1412, 1413, 2170
BssMI	GATC	6 cut(s)	1149, 1358, 1595, 1865, 2270, 2383
BssNI	GRCGYC	1 cut(s)	608
BssT1I	CCWWGG	3 cut(s)	226, 1194, 2170
Bst2UI	CCWGG	1 cut(s)	14
Bst4CI	ACNGT	6 cut(s)	166, 272, 1805, 1905, 2351, 2628
Bst6I	CTCTTC	4 cut(s)	44, 894, 1263, 1945
BstACI	GRCGYC	1 cut(s)	608
BstAFI	CTTAAG	2 cut(s)	1700, 2318
BstAPI	GCANNNNNTGC	1 cut(s)	2057
BstBI	TTCGAA	1 cut(s)	410
BstC8I	GCNNGC	8 cut(s)	30, 694, 811, 1118, 1707, 2078, 2099, 2282
BstDEI	CTNAG	3 cut(s)	198, 625, 1045
BstDSI	CCRYGG	2 cut(s)	1194, 2170
BstENI	CCTNNNNNAGG	1 cut(s)	123
BstF5I	GGATG	5 cut(s)	368, 751, 1086, 1324, 2032
BstFNI	CGCG	1 cut(s)	995
BstH2I	RGCGCY	1 cut(s)	611
BstHHI	GCGC	4 cut(s)	610, 1070, 1342, 2447
BstKTI	GATC	6 cut(s)	1152, 1361, 1598, 1868, 2273, 2386
BstMAI	GTCTC	2 cut(s)	1088, 1794
BstMBI	GATC	6 cut(s)	1149, 1358, 1595, 1865, 2270, 2383
BstNI	CCWGG	1 cut(s)	14
BstNSI	RCATGY	5 cut(s)	1019, 1074, 2076, 2080, 2101
BstSCI	CCNGG	3 cut(s)	12, 1411, 1412
BstSFI	CTRYAG	4 cut(s)	561, 1605, 1830, 2049
BstUI	CGCG	1 cut(s)	995
BstV2I	GAAGAC	3 cut(s)	549, 1535, 1749
BstXI	CCANNNNNNTGG	3 cut(s)	363, 2126, 2162
BsuI	GTATCC	2 cut(s)	1532, 2018
BsuRI	GGCC	4 cut(s)	289, 355, 692, 2154
BtgI	CCRYGG	2 cut(s)	1194, 2170
BtsCI	GGATG	5 cut(s)	368, 751, 1086, 1324, 2032
BtsI	GCAGTG	4 cut(s)	588, 2332, 2476, 2548
BtsIMutI	CAGTG	7 cut(s)	150, 588, 2015, 2332, 2347, 2476, 2548
BveI	ACCTGC	2 cut(s)	116, 554
Cac8I	GCNNGC	8 cut(s)	30, 694, 811, 1118, 1707, 2078, 2099, 2282
CaiI	CAGNNNCTG	2 cut(s)	512, 1613
CciI	TCATGA	2 cut(s)	445, 2380
CfoI	GCGC	4 cut(s)	610, 1070, 1342, 2447
Cfr10I	RCCGGY	1 cut(s)	692
Cfr13I	GGNCC	1 cut(s)	970
Cfr9I	CCCGGG	1 cut(s)	1412
Csp6I	GTAC	1 cut(s)	1215
CviQI	GTAC	1 cut(s)	1215
DdeI	CTNAG	3 cut(s)	198, 625, 1045
DinI	GGCGCC	1 cut(s)	609
DpnI	GATC	6 cut(s)	1151, 1360, 1597, 1867, 2272, 2385
DpnII	GATC	6 cut(s)	1149, 1358, 1595, 1865, 2270, 2383
DraI	TTTAAA	1 cut(s)	1771
EaeI	YGGCCR	3 cut(s)	287, 353, 2152
Eam1104I	CTCTTC	4 cut(s)	44, 894, 1263, 1945
EarI	CTCTTC	4 cut(s)	44, 894, 1263, 1945
Ecl136II	GAGCTC	2 cut(s)	1709, 2439
Eco130I	CCWWGG	3 cut(s)	226, 1194, 2170
Eco24I	GRGCYC	2 cut(s)	1711, 2441
Eco31I	GGTCTC	1 cut(s)	1088
Eco32I	GATATC	1 cut(s)	1450
Eco47I	GGWCC	1 cut(s)	970
Eco53kI	GAGCTC	2 cut(s)	1709, 2439
Eco57I	CTGAAG	2 cut(s)	882, 1761
Eco88I	CYCGRG	3 cut(s)	1412, 1712, 2387
EcoICRI	GAGCTC	2 cut(s)	1709, 2439
EcoNI	CCTNNNNNAGG	1 cut(s)	123
EcoRII	CCWGG	1 cut(s)	12
EcoRV	GATATC	1 cut(s)	1450
EcoT14I	CCWWGG	3 cut(s)	226, 1194, 2170
EcoT22I	ATGCAT	1 cut(s)	2078
EcoT38I	GRGCYC	2 cut(s)	1711, 2441
EgeI	GGCGCC	1 cut(s)	609
EheI	GGCGCC	1 cut(s)	609
ErhI	CCWWGG	3 cut(s)	226, 1194, 2170
Esp3I	CGTCTC	1 cut(s)	1794
FalI	AAGNNNNNCTT	2 cut(s)	41, 73
FaqI	GGGAC	4 cut(s)	281, 496, 2083, 2274
FauNDI	CATATG	1 cut(s)	298
FbaI	TGATCA	1 cut(s)	1149
FokI	GGATG	5 cut(s)	375, 758, 1073, 1311, 2019
FriOI	GRGCYC	2 cut(s)	1711, 2441
FspBI	CTAG	3 cut(s)	236, 974, 2286
FspI	TGCGCA	1 cut(s)	2446
GlaI	GCGC	4 cut(s)	609, 1069, 1341, 2446
GsaI	CCCAGC	1 cut(s)	968
GsuI	CTGGAG	2 cut(s)	783, 1587
HaeII	RGCGCY	1 cut(s)	611
HaeIII	GGCC	4 cut(s)	289, 355, 692, 2154
HapII	CCGG	4 cut(s)	439, 553, 693, 1413
HhaI	GCGC	4 cut(s)	610, 1070, 1342, 2447
Hin1I	GRCGYC	1 cut(s)	608
Hin6I	GCGC	4 cut(s)	608, 1068, 1340, 2445
HinP1I	GCGC	4 cut(s)	608, 1068, 1340, 2445
HincII	GTYRAC	2 cut(s)	1348, 2520
HindII	GTYRAC	2 cut(s)	1348, 2520
HindIII	AAGCTT	2 cut(s)	2302, 2315
HinfI	GANTC	8 cut(s)	767, 794, 1000, 1174, 1569, 1755, 2390, 2422
HpaII	CCGG	4 cut(s)	439, 553, 693, 1413
HphI	GGTGA	7 cut(s)	526, 1009, 1075, 1172, 1241, 1252, 2465
Hpy166II	GTNNAC	5 cut(s)	534, 1021, 1348, 2240, 2520
Hpy188I	TCNGA	7 cut(s)	48, 88, 99, 432, 1149, 2212, 2275
Hpy8I	GTNNAC	5 cut(s)	534, 1021, 1348, 2240, 2520
Hpy99I	CGWCG	1 cut(s)	595
HpyCH4III	ACNGT	6 cut(s)	166, 272, 1805, 1905, 2351, 2628
HpyCH4IV	ACGT	1 cut(s)	284
HpyF3I	CTNAG	3 cut(s)	198, 625, 1045
HpySE526I	ACGT	1 cut(s)	284
Hsp92I	GRCGYC	1 cut(s)	608
HspAI	GCGC	4 cut(s)	608, 1068, 1340, 2445
KasI	GGCGCC	1 cut(s)	607
KroI	GCCGGC	1 cut(s)	692
KroNI	GCCGGC	1 cut(s)	694
Ksp22I	TGATCA	1 cut(s)	1149
Kzo9I	GATC	6 cut(s)	1149, 1358, 1595, 1865, 2270, 2383
LguI	GCTCTTC	2 cut(s)	44, 1945
LmnI	GCTCC	4 cut(s)	377, 555, 1029, 1621
LweI	GCATC	4 cut(s)	1327, 1576, 1804, 2041
MaeI	CTAG	3 cut(s)	236, 974, 2286
MaeII	ACGT	1 cut(s)	284
MaeIII	GTNAC	7 cut(s)	272, 318, 566, 1105, 1160, 1460, 2327
MalI	GATC	6 cut(s)	1151, 1360, 1597, 1867, 2272, 2385
MbiI	CCGCTC	2 cut(s)	380, 2038
MboI	GATC	6 cut(s)	1149, 1358, 1595, 1865, 2270, 2383
MfeI	CAATTG	3 cut(s)	303, 1024, 1506
MhlI	GDGCHC	3 cut(s)	1711, 2441, 2565
MlsI	TGGCCA	2 cut(s)	289, 2154
MluNI	TGGCCA	2 cut(s)	289, 2154
Mly113I	GGCGCC	1 cut(s)	608
MlyI	GAGTC	4 cut(s)	788, 1578, 2399, 2431
MmeI	TCCRAC	3 cut(s)	1526, 1777, 1963
Mox20I	TGGCCA	2 cut(s)	289, 2154
Mph1103I	ATGCAT	1 cut(s)	2078
MroNI	GCCGGC	1 cut(s)	692
MroXI	GAANNNNTTC	2 cut(s)	1422, 1497
MscI	TGGCCA	2 cut(s)	289, 2154
MslI	CAYNNNNRTG	8 cut(s)	361, 1075, 1689, 2081, 2124, 2160, 2352, 2409
Msp20I	TGGCCA	2 cut(s)	289, 2154
MspA1I	CMGCKG	2 cut(s)	968, 1007
MspCI	CTTAAG	2 cut(s)	1700, 2318
MspI	CCGG	4 cut(s)	439, 553, 693, 1413
MspR9I	CCNGG	3 cut(s)	14, 1413, 1414
MunI	CAATTG	3 cut(s)	303, 1024, 1506
MvaI	CCWGG	1 cut(s)	14
MvnI	CGCG	1 cut(s)	995
NaeI	GCCGGC	1 cut(s)	694
NarI	GGCGCC	1 cut(s)	608
NciI	CCSGG	2 cut(s)	1413, 1414
NcoI	CCATGG	2 cut(s)	1194, 2170
NdeI	CATATG	1 cut(s)	298
NdeII	GATC	6 cut(s)	1149, 1358, 1595, 1865, 2270, 2383
NgoMIV	GCCGGC	1 cut(s)	692
NlaIV	GGNNCC	4 cut(s)	551, 609, 1558, 2116
NmuCI	GTSAC	3 cut(s)	1105, 1160, 2327
NsbI	TGCGCA	1 cut(s)	2446
NsiI	ATGCAT	1 cut(s)	2078
NspI	RCATGY	5 cut(s)	1019, 1074, 2076, 2080, 2101
NspV	TTCGAA	1 cut(s)	410
PaeI	GCATGC	2 cut(s)	2080, 2101
PaeR7I	CTCGAG	2 cut(s)	1712, 2387
PagI	TCATGA	2 cut(s)	445, 2380
PaqCI	CACCTGC	1 cut(s)	116
PasI	CCCWGGG	1 cut(s)	13
PciI	ACATGT	1 cut(s)	1015
PciSI	GCTCTTC	2 cut(s)	44, 1945
PdiI	GCCGGC	1 cut(s)	694
PdmI	GAANNNNTTC	2 cut(s)	1422, 1497
PfeI	GAWTC	4 cut(s)	767, 1000, 1174, 1755
PflFI	GACNNNGTC	1 cut(s)	2178
PflMI	CCANNNNNTGG	1 cut(s)	804
PleI	GAGTC	4 cut(s)	788, 1577, 2398, 2430
PluTI	GGCGCC	1 cut(s)	611
PpsI	GAGTC	4 cut(s)	788, 1577, 2398, 2430
PscI	ACATGT	1 cut(s)	1015
Psp124BI	GAGCTC	2 cut(s)	1711, 2441
Psp6I	CCWGG	1 cut(s)	12
PspFI	CCCAGC	1 cut(s)	964
PspGI	CCWGG	1 cut(s)	12
PspN4I	GGNNCC	4 cut(s)	551, 609, 1558, 2116
PspPI	GGNCC	1 cut(s)	970
PsrI	GAACNNNNNNTAC	2 cut(s)	1002, 1034
PstI	CTGCAG	3 cut(s)	565, 1609, 2053
PstNI	CAGNNNCTG	2 cut(s)	512, 1613
PsyI	GACNNNGTC	1 cut(s)	2178
PvuII	CAGCTG	1 cut(s)	1007
RsaI	GTAC	1 cut(s)	1216
RsaNI	GTAC	1 cut(s)	1215
RseI	CAYNNNNRTG	8 cut(s)	361, 1075, 1689, 2081, 2124, 2160, 2352, 2409
SacI	GAGCTC	2 cut(s)	1711, 2441
SapI	GCTCTTC	2 cut(s)	44, 1945
Sau3AI	GATC	6 cut(s)	1149, 1358, 1595, 1865, 2270, 2383
Sau96I	GGNCC	1 cut(s)	970
SchI	GAGTC	4 cut(s)	788, 1578, 2399, 2431
ScrFI	CCNGG	3 cut(s)	14, 1413, 1414
SduI	GDGCHC	3 cut(s)	1711, 2441, 2565
SfaNI	GCATC	4 cut(s)	1327, 1576, 1804, 2041
SfcI	CTRYAG	4 cut(s)	561, 1605, 1830, 2049
SfoI	GGCGCC	1 cut(s)	609
Sfr274I	CTCGAG	2 cut(s)	1712, 2387
SfuI	TTCGAA	1 cut(s)	410
SinI	GGWCC	1 cut(s)	970
SlaI	CTCGAG	2 cut(s)	1712, 2387
SmaI	CCCGGG	1 cut(s)	1414
SmiMI	CAYNNNNRTG	8 cut(s)	361, 1075, 1689, 2081, 2124, 2160, 2352, 2409
SmlI	CTYRAG	8 cut(s)	113, 338, 1700, 1712, 1878, 2318, 2387, 2618
SmoI	CTYRAG	8 cut(s)	113, 338, 1700, 1712, 1878, 2318, 2387, 2618
SphI	GCATGC	2 cut(s)	2080, 2101
SspDI	GGCGCC	1 cut(s)	607
SspMI	CTAG	3 cut(s)	236, 974, 2286
SstI	GAGCTC	2 cut(s)	1711, 2441
StyD4I	CCNGG	3 cut(s)	12, 1411, 1412
StyI	CCWWGG	3 cut(s)	226, 1194, 2170
TaaI	ACNGT	6 cut(s)	166, 272, 1805, 1905, 2351, 2628
TaiI	ACGT	1 cut(s)	287
TaqI	TCGA	7 cut(s)	410, 518, 897, 1594, 1713, 2388, 2543
TauI	GCSGC	3 cut(s)	1133, 1679, 2556
TfiI	GAWTC	4 cut(s)	767, 1000, 1174, 1755
TscAI	CASTG	7 cut(s)	150, 588, 2022, 2332, 2354, 2483, 2555
TseFI	GTSAC	3 cut(s)	1105, 1160, 2327
Tsp45I	GTSAC	3 cut(s)	1105, 1160, 2327
TspDTI	ATGAA	5 cut(s)	434, 843, 925, 1415, 2370
TspGWI	ACGGA	1 cut(s)	1065
TspMI	CCCGGG	1 cut(s)	1412
TspRI	CASTG	7 cut(s)	150, 588, 2022, 2332, 2354, 2483, 2555
Tth111I	GACNNNGTC	1 cut(s)	2178
Van91I	CCANNNNNTGG	1 cut(s)	804
Vha464I	CTTAAG	2 cut(s)	1700, 2318
VpaK11BI	GGWCC	1 cut(s)	970
XagI	CCTNNNNNAGG	1 cut(s)	123
XapI	RAATTY	6 cut(s)	433, 1232, 1405, 1766, 2296, 2591
XceI	RCATGY	5 cut(s)	1019, 1074, 2076, 2080, 2101
XhoI	CTCGAG	2 cut(s)	1712, 2387
XmaI	CCCGGG	1 cut(s)	1412
XmnI	GAANNNNTTC	2 cut(s)	1422, 1497
XspI	CTAG	3 cut(s)	236, 974, 2286
Zsp2I	ATGCAT	1 cut(s)	2078

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.