Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001793

Physical Location & Seq

Genomic Coordinates Reverse (-)

28384003 .. 28393565

Gene Structure 9563 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1252700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3132 bp

ATGGCTTCTGCCCAGGTCAAAGAAAAGGTAGCAGCCAAAAAATTTCAGAAGAGCTACTTTGATGTCTTGGGGTTGTGCTGCTCATCAGAAGTTCCACTCATAGAGAATATCCTCAAGCCTCTTGAAGGCGTGAAAGAGGTTTCAGTGATTGTGCCTTCCAGAACTGTAATTGTTGTCCATGATATCCTCCTCATTTCCCAGCTTCAAATTGTTAAGGCATTAAACCAAGCAAGGTTAGAAGCAAATGTGAGAGTGTATGGGGCAGGGGACAGTTACAAGAAAACGTGGCCAAGTCCATATGCAATTGGTAGTGGCGTGTTACTTGTTCTATCATTTCTCAAGTCTGTGTATCGGCCATTAGGGTGGTTGGCCCTTGGAGCGGTGTTCCTTGGCATTTTTCCCATTGCTATGAAAGGTTTTGCGTCAATTCGAAATTTCAGGTTTCATGACATAAACATTCTTGTTATCATTGCCGTGGTTGGAACAATTGCTTTGAAGGACTATACCGAAGCTGCCTCTATTGTCTTTCTGTTCATCATTGCAGAGTGGCTTCAGTCTTGTGCAGGATACAGGGCGAAAGCAGTGATGTCGTCGTTGATGAGCATGGCGCCCCAAAAAGCAGTCTTAGCTGAGAGTGGAGAAGTTGTAGATGTTGATGAGGTTAAGTTGTACACAATTCTTGCTGTTAAAGCCGGCGAAGTTATACCGATTGATGGAATAGTTGTTGAAGGAAAAGGCGAAGTGGATGAGCAAACACTAACCGGGGAATCGTATCCAGTAGCCAAAGAAAAGGACTCCACTGTTTGGGCGGGCACTATCAATCTTAATGGTTATATTAGTGTTAAAACTACAGCGTTAGCTGAAGATTGTGCGGTTGCCAAAATGGCCAAACTTGTCGAAGAGGCTCAAAGCAGCAAAACCAGAACTCAAAGATTCATTGACAAATGCGCAATGTTCTATACCCCAGCGGTCCTAGTCATATCTGTATCCATCGCGGTGATTCCGGCAGCATTACATGTTCACAATTGGAGCAAGTGGTTTCACTTAGCTTTGGTTGTTTTAGTGAGTGCATGTCCTTGCGGCCTCATCCTCTCTACACCCATTGTGACTTTTTGCACACTCACAAAGGCAGCAACATCCGGACTGCTAATCAAAGGAGGTGACTACATTGAGATCCTTGCTAAAGTCAAGATCATGGCTTTTGACAAAACTGGGACAATAACAAGGGGTGAATTTGTGGTGATGGATTTTCAATCTCTTCGTGATGACATTAGCTTGAACACATTGCTTTACTGGGTTGCAAGCATTGAGAGGAAGTCCAGTCATCCAATGGCAGATGCGCTGGTTGACTATGGAAGATCGCTTTCGATTGAGCCAAAACCTGAAAATGTGGAGGAGTTTCAAAATTTTCCCGGGGAAGGTATCCATGGGAAAATTGATGGACAAGATATCTACATTGGAAACAGAAAAATAGCTTTACGAGCTGCTTGTGAAATAGTTCCAACAACTGAAGGTTCTAATGGCGGGAAAACGATCGGATACATATACTCTCGAGGAACCCCTGCCGGAGTCTTTACAATATCTGATGCTTGTCGATCTGGGGCCGCAGAGGCTATCAGAGAGCTCAAGAAGTTGGGTATTAAAACAGCTATGCTCACTGGAGATAGTAATGCTGCCGCATTGCATACAAATGAGCAGCTTAAGCAAGCTCTCGAGGTAGTACATGCAGAACTTCTACCTGAAGATAAGGCAAGAATCGTTAAAGAATTTAAAACGGAAGGAAATACAGCAATGGTTGGAGACGGTATCAATGATGCCCCTGCATTAGCCACAGCGGATATTGGTATCTCAATGGGCATTTCAGGATCAGCCCTTGCTCAAGAAACTGGGAATATAATTTTACTATCAAATGACATTAGGAAACTACCGAAAGCAGTCCAACTGGCGAGAAGAGCTAATAGAAAAGTGGTTGAGAATGTTGTTTTGTCAATCACTACTAAGGTTGGTATCCTTGCTCTGGGATTTGCAGGGCATCCGCTTGTTTGGGCTGCGGTTCTTGCTGATGTTGGGACATGCATGCTTGTTATATTCAACAGCATGCTACTCTTACAAGGAACCCAAAAGCATGGAGGGAATACTTCTGCACCACATGCCCATAAACATGGAAGCCATGGACATAGCCTCTCTCACAAAAACCAACGTTGTTGCTCGGATAGCAAACCCGTGAAAGCCTGTAAACCTCAAAAGTGTTCATCGCAGTGCCAGCCTACACCCTTGAATTCAAGCTTGCCCTGTATGCTTAAGTGCAGTGATGACTTACGCAAGGCACGACACTGTGATGAAGCAAGCTGCATGAAAGTCAATAAAGATCTCGAGTCACAGAATAAACACAATCATGGTTGTTTGAGTCATCATGATTTGAGCTCATGTGCAGAAGGTGATAAAATTCACGAGGCAAAGCACTGCAACGGTTCTGCTTCTTCTTTGGTGGAAATTCAAAAGTTGACAAGCAAAGGTCATTGCCACTCAACGCACTGCAGCAAAGAGCACAGCACGAAGGAAAGTGATGGAGTCCATGAGGAAAAACACTGCAATCATTCTATCTTTTCTTTGGATGAAAGCAAAAGATTGACAGATAGTGACATTTCCCGCTCAAGACACTGCGGAAAGGAGCATAGTAGAAAGGAAGGTGATGCAATACACGAGCCAAAACACTGCAACCATTCTGCTTTTTCAATGGAGGAATGCCAAAAATTGACGAGTACCGGTCATTGCCACTCAACTCACTGTGGCAAAGATCATATTCACAATGAAGCGTTAGGAGAGACGTTTGCCACATGCTGTGATCATCAGCATCACTGTAATCCAGATGAAAACTCACCTCCTAATACGACTATTGATATTGTACCAGGTTGTGACCCAGCAGAATCAGCCCCAACGAATTCTTGCATTGGCTCAGGGACAGTGGAAAAGGAAGCGTGTTGCTCGGAATCAGTAGCAATTCAAGCTTGTGTGTTAGAGAAAAGAGAAGTCGGTGGGTGCTGCAAAAGCTACATGAAGGAATGCTGTGGTAGTCATGGGCATATTGGTTCTAGCTTTAAGGGTTGTTTATCAGAAATCACAATTGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006829 GO:0006950 GO:0008150 GO:0009506 GO:0009636 GO:0010035 GO:0010038 GO:0010043 GO:0015691 GO:0016020 GO:0030001 GO:0030054 GO:0031090 GO:0032025 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055065 GO:0055069 GO:0055076 GO:0055080 GO:0061687 GO:0065007 GO:0065008 GO:0070838 GO:0071585 GO:0071944 GO:0072507 GO:0072511 GO:0097501 GO:0098588 GO:0098754 GO:0098771 GO:0098805 GO:1990170

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

1044

Amino Acids

112.49

Weight (kDa)

6.85

Isoelectric Point (pI)

37.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	205 - 300	3.7e-22	P-type ATPase actuator domain
Hydrolase	PF00702	396 - 613	1.6e-27	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001793)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19110 AT2G19110 AT2G19110 AT4G30110 AT4G30110 AT4G30120 AT4G30120
fragaria_vesca	FvH4_4g07520 FvH4_4g07520 FvH4_4g07520 FvH4_4g07520 FvH4_6g33520 FvH4_6g33520 FvH4_6g33520 FvH4_6g33520
malus_domestica	MD00G1104400.v1.1 MD01G1183400.v1.1 MD01G1183700.v1.1 MD01G1184000.v1.1 MD01G1184300.v1.1 MD13G1244500.v1.1 MD16G1252700.v1.1
prunus_persica	Prupe.1G078700_v2.0.a1 Prupe.1G078700_v2.0.a1 Prupe.1G078700_v2.0.a1
pyrus_communis	pycom13g21550 pycom16g21330
rosa_chinensis	RchiOBHm_Chr2g0143431 RchiOBHm_Chr4g0400101
rosa_laevigata	RLG00000009214 RLG00000020035
rosa_multiflora	Rmu_sc0000037.1_g000006
rosa_roxburghii	Rroxscaffold_2G00101850 Rroxscaffold_5G00345040 Rroxscaffold_5G00345070
rosa_rugosa	Rorug02G0381400 Rorug04G0022600 Rorug04G0022600
rosa_samantha	Rh2AG434400 Rh2BG442200 Rh2CG420300 Rh2DG452400 Rh4BG096400 Rh4CG107500 Rh4DG093000
rosa_wichuraiana	Rw2G035400 Rw4G008180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	949
AccB1I	GGYRCC	1 cut(s)	607
AccB7I	CCANNNNNTGG	1 cut(s)	804
AccBSI	CCGCTC	2 cut(s)	380, 2652
AccII	CGCG	1 cut(s)	995
AccIII	TCCGGA	1 cut(s)	1139
AclI	AACGTT	1 cut(s)	2200
AclWI	GGATC	2 cut(s)	1168, 1873
AcoI	YGGCCR	3 cut(s)	287, 353, 885
AcsI	RAATTY	9 cut(s)	41, 433, 1232, 1405, 1766, 2278, 2445, 2493, 2941
AcuI	CTGAAG	4 cut(s)	536, 882, 1530, 1761
AcyI	GRCGYC	1 cut(s)	608
AfaI	GTAC	4 cut(s)	671, 1722, 2764, 2907
AfiI	CCNNNNNNNGG	6 cut(s)	125, 379, 713, 804, 1418, 2017
AflII	CTTAAG	2 cut(s)	1700, 2300
AflIII	ACRYGT	1 cut(s)	1015
AgeI	ACCGGT	1 cut(s)	2765
AjnI	CCWGG	2 cut(s)	12, 2908
Alw21I	GWGCWC	3 cut(s)	1626, 2426, 2550
Alw26I	GTCTC	2 cut(s)	1794, 2819
AlwI	GGATC	2 cut(s)	1168, 1873
Ama87I	CYCGRG	4 cut(s)	1412, 1551, 1712, 2372
Aor13HI	TCCGGA	1 cut(s)	1139
AoxI	GGCC	6 cut(s)	287, 353, 369, 885, 1081, 1602
ApoI	RAATTY	9 cut(s)	41, 433, 1232, 1405, 1766, 2278, 2445, 2493, 2941
AsiGI	ACCGGT	1 cut(s)	2765
Asp700I	GAANNNNTTC	1 cut(s)	1497
AspLEI	GCGC	3 cut(s)	610, 950, 1342
AspS9I	GGNCC	3 cut(s)	370, 970, 1602
AsuC2I	CCSGG	3 cut(s)	763, 1413, 1414
AsuHPI	GGTGA	7 cut(s)	1009, 1172, 1241, 1252, 2450, 2702, 2871
AsuII	TTCGAA	1 cut(s)	430
AvaI	CYCGRG	4 cut(s)	1412, 1551, 1712, 2372
AvaII	GGWCC	1 cut(s)	970
BaeGI	GKGCMC	1 cut(s)	815
BalI	TGGCCA	2 cut(s)	289, 887
BanI	GGYRCC	1 cut(s)	607
BanII	GRGCYC	2 cut(s)	1626, 2426
BauI	CACGAG	2 cut(s)	2450, 2702
Bbv12I	GWGCWC	3 cut(s)	1626, 2426, 2550
BccI	CCATC	5 cut(s)	707, 998, 1237, 1433, 2561
BceAI	ACGGC	1 cut(s)	458
BcgI	CGANNNNNNTGC	2 cut(s)	570, 604
BciT130I	CCWGG	2 cut(s)	14, 2910
BciVI	GTATCC	6 cut(s)	560, 783, 997, 1433, 1532, 2018
BclI	TGATCA	1 cut(s)	2845
BcnI	CCSGG	3 cut(s)	763, 1413, 1414
BcoDI	GTCTC	2 cut(s)	1794, 2819
BfaI	CTAG	2 cut(s)	974, 3093
BfmI	CTRYAG	2 cut(s)	849, 2536
BfoI	RGCGCY	1 cut(s)	611
BfrI	CTTAAG	2 cut(s)	1700, 2300
BfuI	GTATCC	6 cut(s)	560, 783, 997, 1433, 1532, 2018
BglI	GCCNNNNNGGC	2 cut(s)	884, 1610
BglII	AGATCT	1 cut(s)	2368
Bme1390I	CCNGG	5 cut(s)	14, 763, 1413, 1414, 2910
Bme18I	GGWCC	1 cut(s)	970
BmeT110I	CYCGRG	4 cut(s)	1412, 1551, 1712, 2372
BmgT120I	GGNCC	3 cut(s)	370, 970, 1602
BmiI	GGNNCC	4 cut(s)	609, 1558, 1603, 2116
BmrFI	CCNGG	5 cut(s)	14, 763, 1413, 1414, 2910
BmrI	ACTGGG	3 cut(s)	1221, 1303, 1896
BmsI	GCATC	6 cut(s)	1327, 1576, 1804, 2041, 2683, 2863
BmuI	ACTGGG	3 cut(s)	1221, 1303, 1896
BpmI	CTGGAG	1 cut(s)	1680
Bpu10I	CCTNAGC	1 cut(s)	2956
Bpu14I	TTCGAA	1 cut(s)	430
BpuEI	CTTGAG	5 cut(s)	98, 323, 1610, 1863, 2638
BpuMI	CCSGG	3 cut(s)	763, 1413, 1414
BsaHI	GRCGYC	1 cut(s)	608
BsaJI	CCNNGG	9 cut(s)	12, 373, 388, 474, 762, 1412, 1413, 1426, 2170
BsaWI	WCCGGW	2 cut(s)	1139, 2765
BsaXI	ACNNNNNCTCC	2 cut(s)	630, 660
Bsc4I	CCNNNNNNNGG	6 cut(s)	125, 379, 713, 804, 1418, 2017
Bse118I	RCCGGY	2 cut(s)	692, 2765
Bse1I	ACTGG	7 cut(s)	776, 1216, 1298, 1320, 1663, 1891, 1947
Bse3DI	GCAATG	9 cut(s)	402, 468, 537, 957, 1283, 1679, 1797, 2518, 2770
BseAI	TCCGGA	1 cut(s)	1139
BseBI	CCWGG	2 cut(s)	14, 2910
BseDI	CCNNGG	9 cut(s)	12, 373, 388, 474, 762, 1412, 1413, 1426, 2170
BseGI	GGATG	6 cut(s)	751, 1086, 1136, 1324, 2032, 2620
BseLI	CCNNNNNNNGG	6 cut(s)	125, 379, 713, 804, 1418, 2017
BseMI	GCAATG	9 cut(s)	402, 468, 537, 957, 1283, 1679, 1797, 2518, 2770
BseMII	CTCAG	2 cut(s)	621, 2970
BseNI	ACTGG	7 cut(s)	776, 1216, 1298, 1320, 1663, 1891, 1947
BseRI	GAGGAG	2 cut(s)	179, 1409
BseSI	GKGCMC	1 cut(s)	815
BseYI	CCCAGC	3 cut(s)	198, 964, 2920
BsgI	GTGCAG	4 cut(s)	582, 2127, 2326, 2451
Bsh1236I	CGCG	1 cut(s)	995
Bsh1285I	CGRYCG	1 cut(s)	1536
BshFI	GGCC	6 cut(s)	289, 355, 371, 887, 1083, 1604
BshNI	GGYRCC	1 cut(s)	607
BshTI	ACCGGT	1 cut(s)	2765
BsiEI	CGRYCG	1 cut(s)	1536
BsiHKAI	GWGCWC	3 cut(s)	1626, 2426, 2550
BsiHKCI	CYCGRG	4 cut(s)	1412, 1551, 1712, 2372
BsiSI	CCGG	7 cut(s)	693, 762, 1004, 1140, 1413, 1566, 2766
BslFI	GGGAC	4 cut(s)	281, 1228, 2083, 2974
BslI	CCNNNNNNNGG	6 cut(s)	125, 379, 713, 804, 1418, 2017
BsmAI	GTCTC	2 cut(s)	1794, 2819
BsmBI	CGTCTC	2 cut(s)	1794, 2819
BsmFI	GGGAC	4 cut(s)	281, 1228, 2083, 2974
BsmI	GAATGC	2 cut(s)	2750, 3068
BsnI	GGCC	6 cut(s)	289, 355, 371, 887, 1083, 1604
BsoBI	CYCGRG	4 cut(s)	1412, 1551, 1712, 2372
Bsp119I	TTCGAA	1 cut(s)	430
Bsp1286I	GDGCHC	4 cut(s)	815, 1626, 2426, 2550
Bsp13I	TCCGGA	1 cut(s)	1139
Bsp1407I	TGTACA	1 cut(s)	669
Bsp143I	GATC	9 cut(s)	1173, 1191, 1358, 1533, 1595, 1865, 2368, 2797, 2845
Bsp19I	CCATGG	2 cut(s)	1426, 2170
BspANI	GGCC	6 cut(s)	289, 355, 371, 887, 1083, 1604
BspCNI	CTCAG	2 cut(s)	622, 2969
BspEI	TCCGGA	1 cut(s)	1139
BspFNI	CGCG	1 cut(s)	995
BspHI	TCATGA	2 cut(s)	445, 2413
BspLI	GGNNCC	4 cut(s)	609, 1558, 1603, 2116
BspMAI	CTGCAG	1 cut(s)	2540
BspPI	GGATC	2 cut(s)	1168, 1873
BspQI	GCTCTTC	2 cut(s)	44, 1945
BspT104I	TTCGAA	1 cut(s)	430
BspT107I	GGYRCC	1 cut(s)	607
BspTI	CTTAAG	2 cut(s)	1700, 2300
BsrBI	CCGCTC	2 cut(s)	380, 2652
BsrDI	GCAATG	9 cut(s)	402, 468, 537, 957, 1283, 1679, 1797, 2518, 2770
BsrFI	RCCGGY	2 cut(s)	692, 2765
BsrGI	TGTACA	1 cut(s)	669
BsrI	ACTGG	7 cut(s)	776, 1216, 1298, 1320, 1663, 1891, 1947
BssAI	RCCGGY	2 cut(s)	692, 2765
BssECI	CCNNGG	9 cut(s)	12, 373, 388, 474, 762, 1412, 1413, 1426, 2170
BssMI	GATC	9 cut(s)	1173, 1191, 1358, 1533, 1595, 1865, 2368, 2797, 2845
BssNI	GRCGYC	1 cut(s)	608
BssSI	CACGAG	2 cut(s)	2450, 2702
BssT1I	CCWWGG	4 cut(s)	373, 388, 1426, 2170
Bst2BI	CACGAG	2 cut(s)	2450, 2702
Bst2UI	CCWGG	2 cut(s)	14, 2910
Bst4CI	ACNGT	9 cut(s)	166, 272, 802, 1805, 2336, 2471, 2789, 2861, 2965
Bst6I	CTCTTC	4 cut(s)	44, 894, 1263, 1945
BstACI	GRCGYC	1 cut(s)	608
BstAFI	CTTAAG	2 cut(s)	1700, 2300
BstAPI	GCANNNNNTGC	1 cut(s)	2150
BstAUI	TGTACA	1 cut(s)	669
BstBI	TTCGAA	1 cut(s)	430
BstC8I	GCNNGC	9 cut(s)	694, 811, 1303, 1707, 2078, 2099, 2264, 2288, 2347
BstDEI	CTNAG	5 cut(s)	625, 630, 1045, 1998, 2956
BstDSI	CCRYGG	3 cut(s)	474, 1426, 2170
BstENI	CCTNNNNNAGG	1 cut(s)	123
BstF5I	GGATG	6 cut(s)	751, 1086, 1136, 1324, 2032, 2620
BstFNI	CGCG	1 cut(s)	995
BstH2I	RGCGCY	1 cut(s)	611
BstHHI	GCGC	3 cut(s)	610, 950, 1342
BstKTI	GATC	9 cut(s)	1176, 1194, 1361, 1536, 1598, 1868, 2371, 2800, 2848
BstMAI	GTCTC	2 cut(s)	1794, 2819
BstMBI	GATC	9 cut(s)	1173, 1191, 1358, 1533, 1595, 1865, 2368, 2797, 2845
BstMCI	CGRYCG	1 cut(s)	1536
BstNI	CCWGG	2 cut(s)	14, 2910
BstNSI	RCATGY	8 cut(s)	1019, 1074, 1727, 2076, 2080, 2101, 2153, 2841
BstSCI	CCNGG	5 cut(s)	12, 761, 1411, 1412, 2908
BstSFI	CTRYAG	2 cut(s)	849, 2536
BstSLI	GKGCMC	1 cut(s)	815
BstUI	CGCG	1 cut(s)	995
BstX2I	RGATCY	2 cut(s)	1173, 2368
BstXI	CCANNNNNNTGG	3 cut(s)	363, 2126, 2162
BstYI	RGATCY	2 cut(s)	1173, 2368
BsuI	GTATCC	6 cut(s)	560, 783, 997, 1433, 1532, 2018
BsuRI	GGCC	6 cut(s)	289, 355, 371, 887, 1083, 1604
BtgI	CCRYGG	3 cut(s)	474, 1426, 2170
BtgZI	GCGATG	2 cut(s)	976, 2238
BtsCI	GGATG	6 cut(s)	751, 1086, 1136, 1324, 2032, 2620
BtsI	GCAGTG	8 cut(s)	588, 2264, 2314, 2461, 2533, 2587, 2659, 2713
Cac8I	GCNNGC	9 cut(s)	694, 811, 1303, 1707, 2078, 2099, 2264, 2288, 2347
CciI	TCATGA	2 cut(s)	445, 2413
CfoI	GCGC	3 cut(s)	610, 950, 1342
Cfr10I	RCCGGY	2 cut(s)	692, 2765
Cfr13I	GGNCC	3 cut(s)	370, 970, 1602
Cfr9I	CCCGGG	1 cut(s)	1412
CseI	GACGC	1 cut(s)	411
CsiI	ACCWGGT	1 cut(s)	2908
Csp6I	GTAC	4 cut(s)	670, 1721, 2763, 2906
CspAI	ACCGGT	1 cut(s)	2765
CviQI	GTAC	4 cut(s)	670, 1721, 2763, 2906
DdeI	CTNAG	5 cut(s)	625, 630, 1045, 1998, 2956
DinI	GGCGCC	1 cut(s)	609
DpnI	GATC	9 cut(s)	1175, 1193, 1360, 1535, 1597, 1867, 2370, 2799, 2847
DpnII	GATC	9 cut(s)	1173, 1191, 1358, 1533, 1595, 1865, 2368, 2797, 2845
DraI	TTTAAA	1 cut(s)	1771
EaeI	YGGCCR	3 cut(s)	287, 353, 885
Eam1104I	CTCTTC	4 cut(s)	44, 894, 1263, 1945
EarI	CTCTTC	4 cut(s)	44, 894, 1263, 1945
Ecl136II	GAGCTC	2 cut(s)	1624, 2424
Eco130I	CCWWGG	4 cut(s)	373, 388, 1426, 2170
Eco24I	GRGCYC	2 cut(s)	1626, 2426
Eco32I	GATATC	2 cut(s)	184, 1450
Eco47I	GGWCC	1 cut(s)	970
Eco53kI	GAGCTC	2 cut(s)	1624, 2424
Eco57I	CTGAAG	4 cut(s)	536, 882, 1530, 1761
Eco88I	CYCGRG	4 cut(s)	1412, 1551, 1712, 2372
EcoICRI	GAGCTC	2 cut(s)	1624, 2424
EcoNI	CCTNNNNNAGG	1 cut(s)	123
EcoRI	GAATTC	2 cut(s)	2278, 2941
EcoRII	CCWGG	2 cut(s)	12, 2908
EcoRV	GATATC	2 cut(s)	184, 1450
EcoT14I	CCWWGG	4 cut(s)	373, 388, 1426, 2170
EcoT22I	ATGCAT	1 cut(s)	2078
EcoT38I	GRGCYC	2 cut(s)	1626, 2426
EgeI	GGCGCC	1 cut(s)	609
EheI	GGCGCC	1 cut(s)	609
ErhI	CCWWGG	4 cut(s)	373, 388, 1426, 2170
Esp3I	CGTCTC	2 cut(s)	1794, 2819
FalI	AAGNNNNNCTT	2 cut(s)	41, 73
FaqI	GGGAC	4 cut(s)	281, 1228, 2083, 2974
FauI	CCCGC	3 cut(s)	802, 1517, 2657
FauNDI	CATATG	1 cut(s)	298
FbaI	TGATCA	1 cut(s)	2845
FokI	GGATG	6 cut(s)	758, 1073, 1123, 1311, 2019, 2627
FriOI	GRGCYC	2 cut(s)	1626, 2426
FspBI	CTAG	2 cut(s)	974, 3093
FspI	TGCGCA	1 cut(s)	949
GlaI	GCGC	3 cut(s)	609, 949, 1341
GsaI	CCCAGC	3 cut(s)	202, 968, 2924
GsuI	CTGGAG	1 cut(s)	1680
HaeII	RGCGCY	1 cut(s)	611
HaeIII	GGCC	6 cut(s)	289, 355, 371, 887, 1083, 1604
HapII	CCGG	7 cut(s)	693, 762, 1004, 1140, 1413, 1566, 2766
HgaI	GACGC	1 cut(s)	411
HhaI	GCGC	3 cut(s)	610, 950, 1342
Hin1I	GRCGYC	1 cut(s)	608
Hin6I	GCGC	3 cut(s)	608, 948, 1340
HinP1I	GCGC	3 cut(s)	608, 948, 1340
HincII	GTYRAC	2 cut(s)	1348, 2505
HindII	GTYRAC	2 cut(s)	1348, 2505
HindIII	AAGCTT	2 cut(s)	2284, 3006
HpaII	CCGG	7 cut(s)	693, 762, 1004, 1140, 1413, 1566, 2766
HphI	GGTGA	7 cut(s)	1009, 1172, 1241, 1252, 2450, 2702, 2871
Hpy166II	GTNNAC	5 cut(s)	672, 1021, 1348, 2237, 2505
Hpy188I	TCNGA	8 cut(s)	48, 88, 1538, 1585, 1619, 2212, 2989, 3115
Hpy8I	GTNNAC	5 cut(s)	672, 1021, 1348, 2237, 2505
Hpy99I	CGWCG	1 cut(s)	595
HpyCH4III	ACNGT	9 cut(s)	166, 272, 802, 1805, 2336, 2471, 2789, 2861, 2965
HpyCH4IV	ACGT	3 cut(s)	284, 2200, 2828
HpyF3I	CTNAG	5 cut(s)	625, 630, 1045, 1998, 2956
HpySE526I	ACGT	3 cut(s)	284, 2200, 2828
Hsp92I	GRCGYC	1 cut(s)	608
HspAI	GCGC	3 cut(s)	608, 948, 1340
KasI	GGCGCC	1 cut(s)	607
Kpn2I	TCCGGA	1 cut(s)	1139
KroI	GCCGGC	1 cut(s)	692
KroNI	GCCGGC	1 cut(s)	694
Ksp22I	TGATCA	1 cut(s)	2845
Kzo9I	GATC	9 cut(s)	1173, 1191, 1358, 1533, 1595, 1865, 2368, 2797, 2845
LguI	GCTCTTC	2 cut(s)	44, 1945
LmnI	GCTCC	3 cut(s)	377, 1029, 2671
LweI	GCATC	6 cut(s)	1327, 1576, 1804, 2041, 2683, 2863
MabI	ACCWGGT	1 cut(s)	2908
MaeI	CTAG	2 cut(s)	974, 3093
MaeII	ACGT	3 cut(s)	284, 2200, 2828
MaeIII	GTNAC	7 cut(s)	272, 318, 1105, 1160, 2376, 2639, 2915
MalI	GATC	9 cut(s)	1175, 1193, 1360, 1535, 1597, 1867, 2370, 2799, 2847
MbiI	CCGCTC	2 cut(s)	380, 2652
MboI	GATC	9 cut(s)	1173, 1191, 1358, 1533, 1595, 1865, 2368, 2797, 2845
MboII	GAAGA	8 cut(s)	61, 875, 911, 1250, 1368, 1754, 1962, 2472
MfeI	CAATTG	4 cut(s)	303, 486, 1024, 3123
MflI	RGATCY	2 cut(s)	1173, 2368
MhlI	GDGCHC	4 cut(s)	815, 1626, 2426, 2550
MlsI	TGGCCA	2 cut(s)	289, 887
MluNI	TGGCCA	2 cut(s)	289, 887
Mly113I	GGCGCC	1 cut(s)	608
MlyI	GAGTC	5 cut(s)	788, 1578, 2384, 2416, 2580
MmeI	TCCRAC	4 cut(s)	460, 1526, 1777, 1963
Mox20I	TGGCCA	2 cut(s)	289, 887
Mph1103I	ATGCAT	1 cut(s)	2078
MroI	TCCGGA	1 cut(s)	1139
MroNI	GCCGGC	1 cut(s)	692
MroXI	GAANNNNTTC	1 cut(s)	1497
MscI	TGGCCA	2 cut(s)	289, 887
MslI	CAYNNNNRTG	9 cut(s)	361, 407, 473, 995, 1689, 2160, 2257, 2337, 2394
Msp20I	TGGCCA	2 cut(s)	289, 887
MspA1I	CMGCKG	2 cut(s)	968, 1835
MspCI	CTTAAG	2 cut(s)	1700, 2300
MspI	CCGG	7 cut(s)	693, 762, 1004, 1140, 1413, 1566, 2766
MspR9I	CCNGG	5 cut(s)	14, 763, 1413, 1414, 2910
MunI	CAATTG	4 cut(s)	303, 486, 1024, 3123
Mva1269I	GAATGC	2 cut(s)	2750, 3068
MvaI	CCWGG	2 cut(s)	14, 2910
MvnI	CGCG	1 cut(s)	995
NaeI	GCCGGC	1 cut(s)	694
NarI	GGCGCC	1 cut(s)	608
NciI	CCSGG	3 cut(s)	763, 1413, 1414
NcoI	CCATGG	2 cut(s)	1426, 2170
NdeI	CATATG	1 cut(s)	298
NdeII	GATC	9 cut(s)	1173, 1191, 1358, 1533, 1595, 1865, 2368, 2797, 2845
NgoMIV	GCCGGC	1 cut(s)	692
NlaIV	GGNNCC	4 cut(s)	609, 1558, 1603, 2116
NmuCI	GTSAC	5 cut(s)	1105, 1160, 2376, 2639, 2915
NsbI	TGCGCA	1 cut(s)	949
NsiI	ATGCAT	1 cut(s)	2078
NspI	RCATGY	8 cut(s)	1019, 1074, 1727, 2076, 2080, 2101, 2153, 2841
NspV	TTCGAA	1 cut(s)	430
PaeI	GCATGC	2 cut(s)	2080, 2101
PaeR7I	CTCGAG	3 cut(s)	1551, 1712, 2372
PagI	TCATGA	2 cut(s)	445, 2413
PciI	ACATGT	1 cut(s)	1015
PciSI	GCTCTTC	2 cut(s)	44, 1945
PcsI	WCGNNNNNNNCGW	1 cut(s)	2326
PctI	GAATGC	2 cut(s)	2750, 3068
PdiI	GCCGGC	1 cut(s)	694
PdmI	GAANNNNTTC	1 cut(s)	1497
PfeI	GAWTC	6 cut(s)	767, 933, 1000, 1755, 2927, 2990
PflMI	CCANNNNNTGG	1 cut(s)	804
PinAI	ACCGGT	1 cut(s)	2765
Ple19I	CGATCG	1 cut(s)	1536
PleI	GAGTC	5 cut(s)	788, 1577, 2383, 2415, 2579
PluTI	GGCGCC	1 cut(s)	611
PpsI	GAGTC	5 cut(s)	788, 1577, 2383, 2415, 2579
PscI	ACATGT	1 cut(s)	1015
Psp124BI	GAGCTC	2 cut(s)	1626, 2426
Psp1406I	AACGTT	1 cut(s)	2200
Psp6I	CCWGG	2 cut(s)	12, 2908
PspFI	CCCAGC	3 cut(s)	198, 964, 2920
PspGI	CCWGG	2 cut(s)	12, 2908
PspN4I	GGNNCC	4 cut(s)	609, 1558, 1603, 2116
PspPI	GGNCC	3 cut(s)	370, 970, 1602
PstI	CTGCAG	1 cut(s)	2540
PsuI	RGATCY	2 cut(s)	1173, 2368
PvuI	CGATCG	1 cut(s)	1536
RsaI	GTAC	4 cut(s)	671, 1722, 2764, 2907
RsaNI	GTAC	4 cut(s)	670, 1721, 2763, 2906
RseI	CAYNNNNRTG	9 cut(s)	361, 407, 473, 995, 1689, 2160, 2257, 2337, 2394
SacI	GAGCTC	2 cut(s)	1626, 2426
SapI	GCTCTTC	2 cut(s)	44, 1945
Sau3AI	GATC	9 cut(s)	1173, 1191, 1358, 1533, 1595, 1865, 2368, 2797, 2845
Sau96I	GGNCC	3 cut(s)	370, 970, 1602
SchI	GAGTC	5 cut(s)	788, 1578, 2384, 2416, 2580
ScrFI	CCNGG	5 cut(s)	14, 763, 1413, 1414, 2910
SduI	GDGCHC	4 cut(s)	815, 1626, 2426, 2550
SexAI	ACCWGGT	1 cut(s)	2908
SfaNI	GCATC	6 cut(s)	1327, 1576, 1804, 2041, 2683, 2863
SfcI	CTRYAG	2 cut(s)	849, 2536
SfoI	GGCGCC	1 cut(s)	609
Sfr274I	CTCGAG	3 cut(s)	1551, 1712, 2372
SfuI	TTCGAA	1 cut(s)	430
SinI	GGWCC	1 cut(s)	970
SlaI	CTCGAG	3 cut(s)	1551, 1712, 2372
SmaI	CCCGGG	1 cut(s)	1414
SmiMI	CAYNNNNRTG	9 cut(s)	361, 407, 473, 995, 1689, 2160, 2257, 2337, 2394
SphI	GCATGC	2 cut(s)	2080, 2101
SspDI	GGCGCC	1 cut(s)	607
SspMI	CTAG	2 cut(s)	974, 3093
SstI	GAGCTC	2 cut(s)	1626, 2426
StyD4I	CCNGG	5 cut(s)	12, 761, 1411, 1412, 2908
StyI	CCWWGG	4 cut(s)	373, 388, 1426, 2170
TaaI	ACNGT	9 cut(s)	166, 272, 802, 1805, 2336, 2471, 2789, 2861, 2965
TaiI	ACGT	3 cut(s)	287, 2203, 2831
TaqI	TCGA	7 cut(s)	430, 897, 1367, 1552, 1594, 1713, 2373
TatI	WGTACW	2 cut(s)	669, 1720
TauI	GCSGC	3 cut(s)	1083, 1607, 1679
TfiI	GAWTC	6 cut(s)	767, 933, 1000, 1755, 2927, 2990
TseFI	GTSAC	5 cut(s)	1105, 1160, 2376, 2639, 2915
Tsp45I	GTSAC	5 cut(s)	1105, 1160, 2376, 2639, 2915
TspGWI	ACGGA	1 cut(s)	1790
TspMI	CCCGGG	1 cut(s)	1412
Van91I	CCANNNNNTGG	1 cut(s)	804
Vha464I	CTTAAG	2 cut(s)	1700, 2300
VpaK11BI	GGWCC	1 cut(s)	970
XagI	CCTNNNNNAGG	1 cut(s)	123
XapI	RAATTY	9 cut(s)	41, 433, 1232, 1405, 1766, 2278, 2445, 2493, 2941
XceI	RCATGY	8 cut(s)	1019, 1074, 1727, 2076, 2080, 2101, 2153, 2841
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1327
XhoI	CTCGAG	3 cut(s)	1551, 1712, 2372
XmaI	CCCGGG	1 cut(s)	1412
XmnI	GAANNNNTTC	1 cut(s)	1497
XspI	CTAG	2 cut(s)	974, 3093
Zsp2I	ATGCAT	1 cut(s)	2078

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.