Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0005465

Physical Location & Seq

Genomic Coordinates Reverse (-)

28238682 .. 28240814

Gene Structure 2133 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1131200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2133 bp

ATGTTTCAAATCATGGAGATCCCACTCTTGCGCTACGAAAATATAGCGTTGGATCAGTTCCAGAGCGGATCCAGCTTACATTTTTCGTTCTCGGATCCTAAGTTCTTCAGGCGGAGATCGTTATCTCTGTTTTCTGGGTACTGTTTCCAATTCAGTCGACGAAAATGGAGGAACCCATTGCTGCATATCAAGTGTTCTTCCGTGGAGCAGGAGCTGAAGCCGCGTCCGAAGCCCGTGCCGTCGAAAATCGAGGTCGATGAGCCGAAGGCGGCGCCTTTGGAAGATACCCAGATCGTGAAACGCAATTCTGGGCTTTGTAGTCAGATTGAGAAGCTGGTTTTGTGTAGTCGGTACCGGGAAGCGCTTGAATTGTTCGAGATTTTGGAGTTTGAAGGTGGGTATGAATTGGCGGGTCATACGTACGACGCTTTGGTGAGTGCTTGCATTAGTTTGAAATCGATTAGGGGGGTTAAGAGGGTTTCTAGTTACATGATTAGTAATGGGTTTGAATTGGATCAGTATATGAGGAACAGGGTGCTTCTTATGCATGTGAAATGCGGGATGATGATTGATGCACGTAGATTGTTCGAGGAAATGCCTGAGAGAAACCAGGTTTCTTGGAACACGATAATCGGGGGCCTTGTGGATTCGGGGAATTTCATGGAGGCCTTCCATTTGTTTCAAATTATGTGGCACGAATTTTCTGACGCTGGATCGCGGACTTTTGCCGCCATGATTCGGGCATCAGCTGGTTTGGCACTCATTTTTGCTGGAAAACAGTTTCATTCCTGTTGTTTGAAGATGGGGCTGGGTTCTGATATTTTTGTGCCTTGTGCGTTGATCGATATGTACAGCAAGTGTGGGAGTATCGAAGATGCTCAATGTGTTTTCGATGAGATGCCGCGGAAGACAACAGTAGGATGGAATACTATCATTGCAGGTTATGCACTTCATGGTTACAGCGAGGAAGCTCTGAGTATGTACTATGACATGCGTGATTCTGGTGTTAAGATGGACCATTTCACATTTTCTATGATCATAAGAATCTGTGCAAGGTTGGCTTCACCGGAACATGCTAAGCAAGCTCATGCAGGTCTAGTTCGTCACGGTTTTGGGTTAGATATAGTGGCGAACACATCACTTGTAGACTTCTATTGCAAATGGGGAAGAGTAGAAGACGCCCGTCATGTTTTTGACCAGATGCCTCTCAAGAATGTTATATCTTGGAATGCCTTGATCGCTGGATATGGTAATCATGGTCGGGGTGACGAGGCTGTTGAGATGTTTGAGAAGATGCTTCAGGAAGGAATGGTACCTAACCATGTTACATTTCTTGCTGTATTGTCTGCTTGCAGTCATTCTGGTTTATCAGAATGCGGATGGGAGATTTTTGACGCAATGAGCAGGGATCACAAGATCAAGCCCCGGGCAATGCATTATGCTTGTATGATTGAATTGCTAGGTCGAGAAGGGCTTTTAGATGAAGCTTATGAACTATTAAGAGACGCTCCCTTTGATCCCACAGCAAATATGTGGGCTGCCCTGCTAACAGCTTGTCGAGTCCATGAGAATTTAGAGCTCGGAAAATTCGCTGCAGAGAAACTTTATGGGATGGAACCTGATAAGCTAAGTAATTATATTGTGCTCTTAAATATATACAACAGTTCTGGGAAGTTGAAGGAGGCTGCCGATGTTGTTCAGACTTTAAGAAGAAAGGGTTTGAGAATGCTTCCGGCATGCACTTGGATTGAAATTAAAAAACAGGTAAATATTTTTCGTTCTGGAGACAAAAGCCACGCTGAAACAAGAGAGATTTACCAGAAAGTGGATGACTTGATGATCAAGATTTCAAAACACGGTTACATCCCTGAGGAGAAGTGTCCACTTCCTGATGTCGATGAACATGAGCAGACGTCGTTTTACCACAGCGAGAAACTAGCCATAGCTTATGGTCTGATCAGTACTTCGGATGGGACGCCACTGCAAATTGTGCAGAGTCACCGTATTTGCGGAGACTGCCATTCTGCGGTTATGTTAATTGCTAGGGTTACGGGAAGGGAAATCGTAGTGAGGGACGCCAGCAGATTCCACCATTTCAAGGATGGGAGTTGCTCTTGTGGAAATTACTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

711

Amino Acids

80.6

Weight (kDa)

6.54

Isoelectric Point (pI)

48.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	304 - 352	1.9e-11	PPR repeat family
PPR	PF01535	308 - 336	2.5e-06	PPR repeat
PPR_2	PF13041	404 - 452	7e-13	PPR repeat family
PPR_1	PF12854	405 - 432	6.4e-06	PPR repeat
PPR	PF01535	407 - 436	7.5e-09	PPR repeat
E_motif	PF20431	522 - 584	6.2e-11	E motif
DYW_deaminase	PF14432	620 - 710	1.8e-35	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005465)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G50390
fragaria_vesca	FvH4_6g48290
malus_domestica	MD00G1131200.v1.1 MD09G1052300.v1.1
prunus_persica	Prupe.3G266400_v2.0.a1
pyrus_communis	pycom111g04370 pycom17g04780
rosa_chinensis	RchiOBHm_Chr2g0168271 RchiOBHm_Chr6g0267531
rosa_laevigata	RLG00000013953 RLG00000013992
rosa_multiflora	Rmu_co8459511.1_g000001 Rmu_sc0003354.1_g000005 Rmu_sc0012005.1_g000008
rosa_roxburghii	Rroxscaffold_2G00083250 Rroxscaffold_7G00200650
rosa_rugosa	Rorug02G0532200 Rorug02G0532200 Rorug02G0532300 Rorug06G0033600
rosa_samantha	Rh2AG601300 Rh2BG614500 Rh2BG614600 Rh6AG155000 Rh6BG155600 Rh6DG140900
rosa_wichuraiana	Rw0G012800 Rw2G049930 Rw6G013300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1916
Acc36I	ACCTGC	2 cut(s)	929, 1082
Acc65I	GGTACC	2 cut(s)	351, 1312
AccB1I	GGYRCC	3 cut(s)	271, 351, 1312
AccB7I	CCANNNNNTGG	1 cut(s)	1825
AccBSI	CCGCTC	1 cut(s)	66
AccI	GTMKAC	2 cut(s)	157, 1146
AccII	CGCG	3 cut(s)	223, 718, 904
AcsI	RAATTY	4 cut(s)	655, 698, 1570, 1586
AcuI	CTGAAG	3 cut(s)	91, 236, 1283
AcyI	GRCGYC	5 cut(s)	272, 1179, 1913, 1976, 2076
AfaI	GTAC	7 cut(s)	140, 353, 422, 851, 983, 1314, 1963
AfeI	AGCGCT	1 cut(s)	363
AfiI	CCNNNNNNNGG	4 cut(s)	738, 1825, 2026, 2098
AjnI	CCWGG	1 cut(s)	609
AloI	GAACNNNNNNTCC	2 cut(s)	86, 118
Alw21I	GWGCWC	2 cut(s)	1581, 1647
Alw26I	GTCTC	3 cut(s)	1497, 1779, 2007
AlwNI	CAGNNNCTG	1 cut(s)	214
Ama87I	CYCGRG	1 cut(s)	1425
Aor51HI	AGCGCT	1 cut(s)	363
AoxI	GGCC	2 cut(s)	637, 666
ApeKI	GCWGC	4 cut(s)	181, 1538, 1592, 1685
ApoI	RAATTY	4 cut(s)	655, 698, 1570, 1586
ArsI	GACNNNNNNTTYG	2 cut(s)	1496, 1528
Asp718I	GGTACC	2 cut(s)	351, 1312
AspLEI	GCGC	3 cut(s)	33, 274, 364
AspS9I	GGNCC	2 cut(s)	637, 1015
AsuC2I	CCSGG	3 cut(s)	356, 1426, 1427
AsuHPI	GGTGA	4 cut(s)	445, 1056, 1277, 1991
AvaI	CYCGRG	1 cut(s)	1425
AvaII	GGWCC	1 cut(s)	1015
AxyI	CCTNAGG	1 cut(s)	1869
BamHI	GGATCC	2 cut(s)	68, 94
BanI	GGYRCC	3 cut(s)	271, 351, 1312
BanII	GRGCYC	1 cut(s)	1581
BarI	GAAGNNNNNNTAC	2 cut(s)	1296, 1328
BbsI	GAAGAC	2 cut(s)	914, 1182
Bbv12I	GWGCWC	2 cut(s)	1581, 1647
BbvI	GCAGC	4 cut(s)	168, 1525, 1579, 1672
BccI	CCATC	7 cut(s)	796, 915, 1006, 1374, 1606, 1964, 2096
BceAI	ACGGC	1 cut(s)	223
BcgI	CGANNNNNNTGC	2 cut(s)	217, 251
BciT130I	CCWGG	1 cut(s)	611
BclI	TGATCA	3 cut(s)	1035, 1839, 1956
BcnI	CCSGG	3 cut(s)	356, 1426, 1427
BcoDI	GTCTC	3 cut(s)	1497, 1779, 2007
BfaI	CTAG	5 cut(s)	483, 1097, 1460, 1937, 2043
BfmI	CTRYAG	1 cut(s)	1593
BfoI	RGCGCY	2 cut(s)	275, 365
BfuAI	ACCTGC	2 cut(s)	929, 1082
BisI	GCNGC	8 cut(s)	182, 221, 270, 729, 902, 1539, 1593, 1686
BlpI	GCTNAGC	1 cut(s)	1077
BlsI	GCNGC	8 cut(s)	183, 222, 271, 730, 903, 1540, 1594, 1687
BmcAI	AGTACT	1 cut(s)	1963
Bme1390I	CCNGG	4 cut(s)	356, 611, 1426, 1427
Bme18I	GGWCC	1 cut(s)	1015
BmeT110I	CYCGRG	1 cut(s)	1425
BmgT120I	GGNCC	2 cut(s)	637, 1015
BmiI	GGNNCC	8 cut(s)	70, 96, 173, 273, 353, 638, 1314, 1617
BmrFI	CCNGG	4 cut(s)	356, 611, 1426, 1427
BmsI	GCATC	6 cut(s)	562, 752, 865, 888, 1191, 1284
BoxI	GACNNNNGTC	1 cut(s)	1182
BpiI	GAAGAC	2 cut(s)	914, 1182
BpmI	CTGGAG	1 cut(s)	1803
Bpu1102I	GCTNAGC	1 cut(s)	1077
BpuEI	CTTGAG	1 cut(s)	1193
BpuMI	CCSGG	3 cut(s)	356, 1426, 1427
Bsa29I	ATCGAT	2 cut(s)	458, 843
BsaAI	YACGTR	2 cut(s)	420, 578
BsaBI	GATNNNNATC	1 cut(s)	121
BsaHI	GRCGYC	5 cut(s)	272, 1179, 1913, 1976, 2076
BsaJI	CCNNGG	4 cut(s)	201, 902, 1424, 1425
BsaWI	WCCGGW	1 cut(s)	1066
Bsc4I	CCNNNNNNNGG	4 cut(s)	738, 1825, 2026, 2098
Bse1I	ACTGG	1 cut(s)	2132
Bse21I	CCTNAGG	1 cut(s)	1869
Bse3DI	GCAATG	4 cut(s)	176, 933, 1404, 1437
Bse8I	GATNNNNATC	1 cut(s)	121
BseBI	CCWGG	1 cut(s)	611
BseCI	ATCGAT	2 cut(s)	458, 843
BseDI	CCNNGG	4 cut(s)	201, 902, 1424, 1425
BseGI	GGATG	8 cut(s)	567, 926, 1385, 1617, 1834, 1863, 1975, 2107
BseJI	GATNNNNATC	1 cut(s)	121
BseLI	CCNNNNNNNGG	4 cut(s)	738, 1825, 2026, 2098
BseMI	GCAATG	4 cut(s)	176, 933, 1404, 1437
BseMII	CTCAG	3 cut(s)	591, 965, 1860
BseNI	ACTGG	1 cut(s)	2132
BseRI	GAGGAG	1 cut(s)	1886
BseXI	GCAGC	4 cut(s)	168, 1525, 1579, 1672
BseYI	CCCAGC	1 cut(s)	808
BsgI	GTGCAG	1 cut(s)	2012
Bsh1236I	CGCG	3 cut(s)	223, 718, 904
BshFI	GGCC	2 cut(s)	639, 668
BshNI	GGYRCC	3 cut(s)	271, 351, 1312
BshVI	ATCGAT	2 cut(s)	458, 843
BsiHKAI	GWGCWC	2 cut(s)	1581, 1647
BsiHKCI	CYCGRG	1 cut(s)	1425
BsiSI	CCGG	4 cut(s)	355, 1067, 1426, 1733
BsiWI	CGTACG	1 cut(s)	420
BslFI	GGGAC	2 cut(s)	1987, 2087
BslI	CCNNNNNNNGG	4 cut(s)	738, 1825, 2026, 2098
BsmAI	GTCTC	3 cut(s)	1497, 1779, 2007
BsmBI	CGTCTC	1 cut(s)	1497
BsmFI	GGGAC	2 cut(s)	1987, 2087
BsmI	GAATGC	3 cut(s)	1234, 1379, 1731
BsnI	GGCC	2 cut(s)	639, 668
BsoBI	CYCGRG	1 cut(s)	1425
Bsp1286I	GDGCHC	2 cut(s)	1581, 1647
Bsp1407I	TGTACA	1 cut(s)	849
Bsp1720I	GCTNAGC	1 cut(s)	1077
BspANI	GGCC	2 cut(s)	639, 668
BspCNI	CTCAG	3 cut(s)	592, 966, 1861
BspDI	ATCGAT	2 cut(s)	458, 843
BspFNI	CGCG	3 cut(s)	223, 718, 904
BspLI	GGNNCC	8 cut(s)	70, 96, 173, 273, 353, 638, 1314, 1617
BspMAI	CTGCAG	1 cut(s)	1597
BspMI	ACCTGC	2 cut(s)	929, 1082
BspT107I	GGYRCC	3 cut(s)	271, 351, 1312
BsrBI	CCGCTC	1 cut(s)	66
BsrDI	GCAATG	4 cut(s)	176, 933, 1404, 1437
BsrGI	TGTACA	1 cut(s)	849
BsrI	ACTGG	1 cut(s)	2132
BssECI	CCNNGG	4 cut(s)	201, 902, 1424, 1425
BssNI	GRCGYC	5 cut(s)	272, 1179, 1913, 1976, 2076
Bst2UI	CCWGG	1 cut(s)	611
Bst4CI	ACNGT	7 cut(s)	143, 780, 916, 1109, 1664, 1859, 2003
Bst6I	CTCTTC	1 cut(s)	1162
BstACI	GRCGYC	5 cut(s)	272, 1179, 1913, 1976, 2076
BstAPI	GCANNNNNTGC	2 cut(s)	944, 1990
BstAUI	TGTACA	1 cut(s)	849
BstBAI	YACGTR	2 cut(s)	420, 578
BstC8I	GCNNGC	5 cut(s)	442, 1083, 1351, 1738, 2080
BstDEI	CTNAG	6 cut(s)	99, 600, 974, 1077, 1628, 1869
BstDSI	CCRYGG	2 cut(s)	201, 902
BstF5I	GGATG	8 cut(s)	567, 926, 1385, 1617, 1834, 1863, 1975, 2107
BstFNI	CGCG	3 cut(s)	223, 718, 904
BstH2I	RGCGCY	2 cut(s)	275, 365
BstHHI	GCGC	3 cut(s)	33, 274, 364
BstMAI	GTCTC	3 cut(s)	1497, 1779, 2007
BstNI	CCWGG	1 cut(s)	611
BstNSI	RCATGY	4 cut(s)	551, 994, 1076, 1740
BstPAI	GACNNNNGTC	1 cut(s)	1182
BstSCI	CCNGG	4 cut(s)	354, 609, 1424, 1425
BstSFI	CTRYAG	1 cut(s)	1593
BstSNI	TACGTA	1 cut(s)	420
BstUI	CGCG	3 cut(s)	223, 718, 904
BstV1I	GCAGC	4 cut(s)	168, 1525, 1579, 1672
BstV2I	GAAGAC	2 cut(s)	914, 1182
BstX2I	RGATCY	3 cut(s)	18, 68, 94
BstYI	RGATCY	3 cut(s)	18, 68, 94
Bsu15I	ATCGAT	2 cut(s)	458, 843
Bsu36I	CCTNAGG	1 cut(s)	1869
BsuRI	GGCC	2 cut(s)	639, 668
BsuTUI	ATCGAT	2 cut(s)	458, 843
BtgI	CCRYGG	2 cut(s)	201, 902
BtsCI	GGATG	8 cut(s)	567, 926, 1385, 1617, 1834, 1863, 1975, 2107
BtsI	GCAGTG	1 cut(s)	1979
BtsIMutI	CAGTG	1 cut(s)	1979
BveI	ACCTGC	2 cut(s)	929, 1082
Cac8I	GCNNGC	5 cut(s)	442, 1083, 1351, 1738, 2080
CaiI	CAGNNNCTG	1 cut(s)	214
CfoI	GCGC	3 cut(s)	33, 274, 364
Cfr13I	GGNCC	2 cut(s)	637, 1015
Cfr42I	CCGCGG	1 cut(s)	905
Cfr9I	CCCGGG	1 cut(s)	1425
ClaI	ATCGAT	2 cut(s)	458, 843
CseI	GACGC	8 cut(s)	212, 434, 716, 1187, 1403, 1514, 1984, 2084
CsiI	ACCWGGT	1 cut(s)	609
Csp6I	GTAC	7 cut(s)	139, 352, 421, 850, 982, 1313, 1962
CspCI	CAANNNNNGTGG	2 cut(s)	671, 706
CviQI	GTAC	7 cut(s)	139, 352, 421, 850, 982, 1313, 1962
DdeI	CTNAG	6 cut(s)	99, 600, 974, 1077, 1628, 1869
DinI	GGCGCC	1 cut(s)	273
Eam1104I	CTCTTC	1 cut(s)	1162
EarI	CTCTTC	1 cut(s)	1162
EciI	GGCGGA	1 cut(s)	127
Ecl136II	GAGCTC	1 cut(s)	1579
Eco105I	TACGTA	1 cut(s)	420
Eco147I	AGGCCT	1 cut(s)	668
Eco24I	GRGCYC	1 cut(s)	1581
Eco47I	GGWCC	1 cut(s)	1015
Eco47III	AGCGCT	1 cut(s)	363
Eco53kI	GAGCTC	1 cut(s)	1579
Eco57I	CTGAAG	3 cut(s)	91, 236, 1283
Eco81I	CCTNAGG	1 cut(s)	1869
Eco88I	CYCGRG	1 cut(s)	1425
EcoICRI	GAGCTC	1 cut(s)	1579
EcoO109I	RGGNCCY	1 cut(s)	637
EcoRII	CCWGG	1 cut(s)	609
EcoT22I	ATGCAT	2 cut(s)	549, 1437
EcoT38I	GRGCYC	1 cut(s)	1581
EgeI	GGCGCC	1 cut(s)	273
EheI	GGCGCC	1 cut(s)	273
Esp3I	CGTCTC	1 cut(s)	1497
FalI	AAGNNNNNCTT	2 cut(s)	1045, 1077
FaqI	GGGAC	2 cut(s)	1987, 2087
FauI	CCCGC	2 cut(s)	403, 551
FbaI	TGATCA	3 cut(s)	1035, 1839, 1956
FblI	GTMKAC	2 cut(s)	157, 1146
Fnu4HI	GCNGC	8 cut(s)	182, 221, 270, 729, 902, 1539, 1593, 1686
FokI	GGATG	8 cut(s)	574, 933, 1392, 1624, 1841, 1850, 1982, 2114
FriOI	GRGCYC	1 cut(s)	1581
Fsp4HI	GCNGC	8 cut(s)	182, 221, 270, 729, 902, 1539, 1593, 1686
FspBI	CTAG	5 cut(s)	483, 1097, 1460, 1937, 2043
GlaI	GCGC	3 cut(s)	32, 273, 363
GluI	GCNGC	8 cut(s)	182, 221, 270, 729, 902, 1539, 1593, 1686
GsaI	CCCAGC	1 cut(s)	812
GsuI	CTGGAG	1 cut(s)	1803
HaeII	RGCGCY	2 cut(s)	275, 365
HaeIII	GGCC	2 cut(s)	639, 668
HapII	CCGG	4 cut(s)	355, 1067, 1426, 1733
HgaI	GACGC	8 cut(s)	212, 434, 716, 1187, 1403, 1514, 1984, 2084
HhaI	GCGC	3 cut(s)	33, 274, 364
Hin1I	GRCGYC	5 cut(s)	272, 1179, 1913, 1976, 2076
Hin6I	GCGC	3 cut(s)	31, 272, 362
HinP1I	GCGC	3 cut(s)	31, 272, 362
HincII	GTYRAC	1 cut(s)	158
HindII	GTYRAC	1 cut(s)	158
HindIII	AAGCTT	1 cut(s)	1485
HinfI	GANTC	7 cut(s)	647, 736, 998, 1044, 1560, 1996, 2085
HpaII	CCGG	4 cut(s)	355, 1067, 1426, 1733
HphI	GGTGA	4 cut(s)	445, 1056, 1277, 1991
Hpy166II	GTNNAC	3 cut(s)	158, 1147, 1883
Hpy188III	TCNNGA	9 cut(s)	61, 295, 376, 1210, 1301, 1466, 1782, 1843, 1889
Hpy8I	GTNNAC	3 cut(s)	158, 1147, 1883
Hpy99I	CGWCG	4 cut(s)	162, 244, 428, 1918
HpyAV	CCTTC	7 cut(s)	259, 386, 679, 1298, 1463, 1672, 2049
HpyCH4III	ACNGT	7 cut(s)	143, 780, 916, 1109, 1664, 1859, 2003
HpyCH4IV	ACGT	3 cut(s)	419, 577, 1913
HpyF3I	CTNAG	6 cut(s)	99, 600, 974, 1077, 1628, 1869
HpySE526I	ACGT	3 cut(s)	419, 577, 1913
Hsp92I	GRCGYC	5 cut(s)	272, 1179, 1913, 1976, 2076
HspAI	GCGC	3 cut(s)	31, 272, 362
KasI	GGCGCC	1 cut(s)	271
KpnI	GGTACC	2 cut(s)	355, 1316
Ksp22I	TGATCA	3 cut(s)	1035, 1839, 1956
KspI	CCGCGG	1 cut(s)	905
LmnI	GCTCC	3 cut(s)	205, 211, 1513
Lsp1109I	GCAGC	4 cut(s)	168, 1525, 1579, 1672
LweI	GCATC	6 cut(s)	562, 752, 865, 888, 1191, 1284
MabI	ACCWGGT	1 cut(s)	609
MaeI	CTAG	5 cut(s)	483, 1097, 1460, 1937, 2043
MaeII	ACGT	3 cut(s)	419, 577, 1913
MaeIII	GTNAC	8 cut(s)	485, 956, 1103, 1265, 1324, 1859, 1997, 2047
MbiI	CCGCTC	1 cut(s)	66
MflI	RGATCY	3 cut(s)	18, 68, 94
MhlI	GDGCHC	2 cut(s)	1581, 1647
Mly113I	GGCGCC	1 cut(s)	272
MlyI	GAGTC	2 cut(s)	1569, 2005
MmeI	TCCRAC	1 cut(s)	30
Mph1103I	ATGCAT	2 cut(s)	549, 1437
MseI	TTAA	7 cut(s)	471, 1008, 1499, 1649, 1706, 1755, 2036
MspA1I	CMGCKG	2 cut(s)	749, 904
MspI	CCGG	4 cut(s)	355, 1067, 1426, 1733
MspR9I	CCNGG	4 cut(s)	356, 611, 1426, 1427
Mva1269I	GAATGC	3 cut(s)	1234, 1379, 1731
MvaI	CCWGG	1 cut(s)	611
MvnI	CGCG	3 cut(s)	223, 718, 904
NarI	GGCGCC	1 cut(s)	272
NciI	CCSGG	3 cut(s)	356, 1426, 1427
NlaIV	GGNNCC	8 cut(s)	70, 96, 173, 273, 353, 638, 1314, 1617
NmuCI	GTSAC	3 cut(s)	1103, 1265, 1997
NsiI	ATGCAT	2 cut(s)	549, 1437
NspI	RCATGY	4 cut(s)	551, 994, 1076, 1740
PaeI	GCATGC	1 cut(s)	1740
PceI	AGGCCT	1 cut(s)	668
PctI	GAATGC	3 cut(s)	1234, 1379, 1731
PfeI	GAWTC	5 cut(s)	647, 736, 998, 1044, 2085
Pfl23II	CGTACG	1 cut(s)	420
PflMI	CCANNNNNTGG	1 cut(s)	1825
PkrI	GCNGC	8 cut(s)	183, 222, 271, 730, 903, 1540, 1594, 1687
PleI	GAGTC	2 cut(s)	1568, 2004
PluTI	GGCGCC	1 cut(s)	275
PpsI	GAGTC	2 cut(s)	1568, 2004
Ppu21I	YACGTR	2 cut(s)	420, 578
PshAI	GACNNNNGTC	1 cut(s)	1182
Psp124BI	GAGCTC	1 cut(s)	1581
Psp6I	CCWGG	1 cut(s)	609
PspFI	CCCAGC	1 cut(s)	808
PspGI	CCWGG	1 cut(s)	609
PspLI	CGTACG	1 cut(s)	420
PspN4I	GGNNCC	8 cut(s)	70, 96, 173, 273, 353, 638, 1314, 1617
PspPI	GGNCC	2 cut(s)	637, 1015
PstI	CTGCAG	1 cut(s)	1597
PstNI	CAGNNNCTG	1 cut(s)	214
PsuI	RGATCY	3 cut(s)	18, 68, 94
PvuII	CAGCTG	1 cut(s)	749
RsaI	GTAC	7 cut(s)	140, 353, 422, 851, 983, 1314, 1963
RsaNI	GTAC	7 cut(s)	139, 352, 421, 850, 982, 1313, 1962
SacI	GAGCTC	1 cut(s)	1581
SacII	CCGCGG	1 cut(s)	905
SalI	GTCGAC	1 cut(s)	156
SaqAI	TTAA	7 cut(s)	471, 1008, 1499, 1649, 1706, 1755, 2036
SatI	GCNGC	8 cut(s)	182, 221, 270, 729, 902, 1539, 1593, 1686
Sau96I	GGNCC	2 cut(s)	637, 1015
ScaI	AGTACT	1 cut(s)	1963
SchI	GAGTC	2 cut(s)	1569, 2005
ScrFI	CCNGG	4 cut(s)	356, 611, 1426, 1427
SduI	GDGCHC	2 cut(s)	1581, 1647
SexAI	ACCWGGT	1 cut(s)	609
SfaNI	GCATC	6 cut(s)	562, 752, 865, 888, 1191, 1284
SfcI	CTRYAG	1 cut(s)	1593
SfoI	GGCGCC	1 cut(s)	273
Sfr303I	CCGCGG	1 cut(s)	905
SgrBI	CCGCGG	1 cut(s)	905
SinI	GGWCC	1 cut(s)	1015
SmaI	CCCGGG	1 cut(s)	1427
SmlI	CTYRAG	1 cut(s)	1208
SmoI	CTYRAG	1 cut(s)	1208
SnaBI	TACGTA	1 cut(s)	420
SphI	GCATGC	1 cut(s)	1740
SseBI	AGGCCT	1 cut(s)	668
SspDI	GGCGCC	1 cut(s)	271
SspI	AATATT	1 cut(s)	1771
SspMI	CTAG	5 cut(s)	483, 1097, 1460, 1937, 2043
SstI	GAGCTC	1 cut(s)	1581
StuI	AGGCCT	1 cut(s)	668
StyD4I	CCNGG	4 cut(s)	354, 609, 1424, 1425
TaaI	ACNGT	7 cut(s)	143, 780, 916, 1109, 1664, 1859, 2003
TaiI	ACGT	3 cut(s)	422, 580, 1916
TatI	WGTACW	3 cut(s)	849, 981, 1961
TauI	GCSGC	4 cut(s)	223, 272, 731, 904
TfiI	GAWTC	5 cut(s)	647, 736, 998, 1044, 2085
Tru1I	TTAA	7 cut(s)	471, 1008, 1499, 1649, 1706, 1755, 2036
Tru9I	TTAA	7 cut(s)	471, 1008, 1499, 1649, 1706, 1755, 2036
TscAI	CASTG	1 cut(s)	1986
TseFI	GTSAC	3 cut(s)	1103, 1265, 1997
TseI	GCWGC	4 cut(s)	181, 1538, 1592, 1685
Tsp45I	GTSAC	3 cut(s)	1103, 1265, 1997
TspDTI	ATGAA	7 cut(s)	417, 649, 773, 941, 1497, 1506, 1914
TspGWI	ACGGA	1 cut(s)	190
TspMI	CCCGGG	1 cut(s)	1425
TspRI	CASTG	1 cut(s)	1986
Van91I	CCANNNNNTGG	1 cut(s)	1825
VpaK11BI	GGWCC	1 cut(s)	1015
XapI	RAATTY	4 cut(s)	655, 698, 1570, 1586
XceI	RCATGY	4 cut(s)	551, 994, 1076, 1740
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2099
XmaI	CCCGGG	1 cut(s)	1425
XmiI	GTMKAC	2 cut(s)	157, 1146
XspI	CTAG	5 cut(s)	483, 1097, 1460, 1937, 2043
ZraI	GACGTC	1 cut(s)	1914
ZrmI	AGTACT	1 cut(s)	1963
Zsp2I	ATGCAT	2 cut(s)	549, 1437

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.