Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0005465

Physical Location & Seq

Genomic Coordinates Reverse (-)

3483243 .. 3485363

Gene Structure 2121 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1052300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2121 bp

ATGGAGATCCCGGTGTTGCGGTATCAAAACTTGGCGTTGGATCAGATCCAGAGCGGATCCAGCTTCCATTTTTCGTTCTCGGATCCTAAGTTCTTAAGGCGGAGATCGTTATCTCTGTTTTCTGGGTACTGTTTCCAGTTCAATCGACTAAAATGGAGGAACCCATTGCCGCATATCAGGTGTTCCTCAGTGGAGCAGGAGCTGAAGCCGCGCCCGAAGCCCGTGCCGTCGAAAATCGACGTCGATGAGCCCAAGGGAGCGCCTTTGGAAGATACCCAGATCGTTAAACGCAATACTGGGCTGTGTAGTCAGATCGAGAAGCTGGTTTTGTGTAATCGGTACCGAGAAGCACTTGAATTGTTTGAGATTTTGGAGTTTGAAGGCGGGTATGAATTGGCGGGTAGTACTTATGACGCGTTGGTGAGCGCTTGTATTATTTTGAAATCGATTAGGGGGGTTAAGAGGGTTTCTAATTACATGATTAGTAATGGGTTTGAGTTGGATCAGTATATGAGGAACAGGGTGCTGCTTATGCATGTGAAATGTGGGATGATGATTGATGCACGTAGGCTTTTCGATGAAATGCCTGTGAGAAACCAGGTTTCTTGGAACACGATAATCGGGGGCCTTGTGGATTCGGGGAATTTCATGGAGGCCTTCCGGTTGTTTCTAATTATGTGGCAGGAGTTTTCTGATGCTGGGTCACGGACTTTTGCCACCATGATTCGGGCATCGGCTGGGTTCGGACTCATTTTTGCTGGAAAACAGTTTCATTCTTGTTGTTTGAAGATGGGGCTAGGTGCTGATATATTCGTGTCTTGTGCGTTGATCGATATGTACAGCAAGTGTGGGAGTATCGAAGATGCTCAGTGGGTTTTTGATGAGATGCCACGGAAGACGACGGTAGGATGGAATACCATCATTGCAGGTTATGCACTTCATGGTTACAGTGAGGAAGCTCTGGGTATGTACTATGACATGCGTGATTCCGGTGTTAAGATGGACCATTTCACATTTTCCATGATCATAAGAATCTGTGCAAGGCTGGCTTCACCGGAACATGCTAAGCAAGCTCATGCAGGTCTAGTTCGTCATGGTTTTGGGTTAGATATAGTGGCCAACACATCACTCGTTGACTTCTATAGCAAATGGGGAAGAATAGAAGACGCTCGTCATGTTTTTGACCAGATGCCTCTCAAGAACGTAATATCTTGGAATGCTTTAATTGCTGGGTATGGTAATCATGGCCGGGGCGATGAGGCTGTTGAGATGTTTGAGAAGATGCTTCGGGAAGGAATGGTACCTAATCATGTTACATTTCTTGCTGTATTGTCTGCTTGCAGTCATTCTGGTTTATCAGAACGTGGATGGGAGATTTTTGAATCAATGAGCAGGGATCACAAGATCAAGCCCCGGGCGATGCATTGTGCTTGTATGATTGAATTGCTAGGTCGAGAGGGGCTTTTGGATGAAGCTTATGCACTAATAATAGACGCTCCCTTTGATCCTACGGCAAATATGTGGGCTGCCCTGCTAACAGCTTGTCGAGTCCATGGGAATTTAGAGCTCGGAAAATTTGCAGCAGAAAAACTTTATGGGATGGAACCTGATAAGCTAAGTAATTATATTGTGCTCTTAAATATATACAACAGTTCTGGGAAGTTGAGGGAGGCTGCTGGTGTCGTTCAGACTCTAAGACGGAAAGGTTTGAGAATGCTTCCAGCATGCACTTGGATCGAAATTAAAAAGCAGGTGCATATTTTTCGTTCTGGAGAAAAAAGCCACGCCAAAACAAGAGAGATCTACCAGAAAGTGGATGACTTGATAAGCAAGATTACAGAACACGGTTACACCCCTGAGGAGAAGTATCTACTTCCTGATGTCGATGAACATGAGCAGACGTCGTTTTACCACAGCGAGAAACTGGCCATCGCTTATGGTCTGATCAATACTTCGGATGGGACACCACTGCAAATTGTGCAGAGTCACCGCATTTGCGGAGACTGCCATTCTGCAGTTAAGTTAATTGCGAGGGTTACGGGAAGGGAAATCATAGTGAGGGACGCCAGCAGATTCCACCATTTCAAGGATGGGAGTTGCTCTTGTGGAAATTACTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

707

Amino Acids

80.02

Weight (kDa)

7.56

Isoelectric Point (pI)

45.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	300 - 348	5.4e-11	PPR repeat family
PPR	PF01535	304 - 332	5.7e-06	PPR repeat
PPR_2	PF13041	400 - 448	4.4e-13	PPR repeat family
PPR_1	PF12854	401 - 428	6.3e-06	PPR repeat
PPR	PF01535	403 - 432	4e-09	PPR repeat
E_motif	PF20431	518 - 580	3.9e-10	E motif
DYW_deaminase	PF14432	616 - 706	2.7e-37	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005465)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G50390
fragaria_vesca	FvH4_6g48290
malus_domestica	MD00G1131200.v1.1 MD09G1052300.v1.1
prunus_persica	Prupe.3G266400_v2.0.a1
pyrus_communis	pycom111g04370 pycom17g04780
rosa_chinensis	RchiOBHm_Chr2g0168271 RchiOBHm_Chr6g0267531
rosa_laevigata	RLG00000013953 RLG00000013992
rosa_multiflora	Rmu_co8459511.1_g000001 Rmu_sc0003354.1_g000005 Rmu_sc0012005.1_g000008
rosa_roxburghii	Rroxscaffold_2G00083250 Rroxscaffold_7G00200650
rosa_rugosa	Rorug02G0532200 Rorug02G0532200 Rorug02G0532300 Rorug06G0033600
rosa_samantha	Rh2AG601300 Rh2BG614500 Rh2BG614600 Rh6AG155000 Rh6BG155600 Rh6DG140900
rosa_wichuraiana	Rw0G012800 Rw2G049930 Rw6G013300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1741
AatII	GACGTC	2 cut(s)	243, 1904
Acc36I	ACCTGC	3 cut(s)	917, 1070, 1741
Acc65I	GGTACC	2 cut(s)	339, 1300
AccB1I	GGYRCC	2 cut(s)	339, 1300
AccB7I	CCANNNNNTGG	1 cut(s)	1813
AccBSI	CCGCTC	1 cut(s)	54
AccII	CGCG	2 cut(s)	211, 416
AciI	CCGC	9 cut(s)	19, 54, 100, 170, 209, 384, 398, 1990, 1998
AcoI	YGGCCR	3 cut(s)	1116, 1246, 1926
AcsI	RAATTY	3 cut(s)	643, 1558, 1574
AcuI	CTGAAG	1 cut(s)	224
AcyI	GRCGYC	3 cut(s)	240, 1901, 2064
AfaI	GTAC	6 cut(s)	128, 341, 406, 839, 971, 1302
AfeI	AGCGCT	1 cut(s)	427
AfiI	CCNNNNNNNGG	3 cut(s)	726, 1813, 2086
AflII	CTTAAG	1 cut(s)	94
AflIII	ACRYGT	1 cut(s)	414
AgsI	TTSAA	8 cut(s)	142, 356, 380, 442, 787, 1382, 1442, 2086
AjnI	CCWGG	1 cut(s)	597
AloI	GAACNNNNNNTCC	2 cut(s)	74, 106
AluBI	AGCT	9 cut(s)	63, 202, 322, 959, 1073, 1475, 1541, 1567, 1615
AluI	AGCT	9 cut(s)	63, 202, 322, 959, 1073, 1475, 1541, 1567, 1615
Alw21I	GWGCWC	2 cut(s)	1569, 1635
Alw26I	GTCTC	1 cut(s)	1995
AlwNI	CAGNNNCTG	1 cut(s)	202
Ama87I	CYCGRG	1 cut(s)	1413
Aor51HI	AGCGCT	1 cut(s)	427
AoxI	GGCC	5 cut(s)	625, 654, 1116, 1246, 1926
ApeKI	GCWGC	4 cut(s)	526, 1526, 1580, 1673
ApoI	RAATTY	3 cut(s)	643, 1558, 1574
ArsI	GACNNNNNNTTYG	2 cut(s)	1484, 1516
Asp718I	GGTACC	2 cut(s)	339, 1300
AspLEI	GCGC	3 cut(s)	213, 262, 428
AspS9I	GGNCC	2 cut(s)	625, 1003
AsuC2I	CCSGG	4 cut(s)	11, 1250, 1414, 1415
AsuHPI	GGTGA	3 cut(s)	433, 1044, 1979
AvaI	CYCGRG	1 cut(s)	1413
AvaII	GGWCC	1 cut(s)	1003
AxyI	CCTNAGG	1 cut(s)	1857
BalI	TGGCCA	2 cut(s)	1118, 1928
BamHI	GGATCC	2 cut(s)	56, 82
BanI	GGYRCC	2 cut(s)	339, 1300
BanII	GRGCYC	2 cut(s)	252, 1569
BarI	GAAGNNNNNNTAC	2 cut(s)	1284, 1316
BbsI	GAAGAC	2 cut(s)	902, 1170
Bbv12I	GWGCWC	2 cut(s)	1569, 1635
BbvI	GCAGC	4 cut(s)	513, 1513, 1592, 1660
BccI	CCATC	9 cut(s)	784, 903, 926, 994, 1362, 1594, 1937, 1952, 2084
BceAI	ACGGC	2 cut(s)	211, 1527
BcgI	CGANNNNNNTGC	6 cut(s)	205, 239, 1717, 1745, 1751, 1779
BciT130I	CCWGG	1 cut(s)	599
BclI	TGATCA	2 cut(s)	1023, 1944
BcnI	CCSGG	4 cut(s)	11, 1250, 1414, 1415
BcoDI	GTCTC	1 cut(s)	1995
BfaI	CTAG	3 cut(s)	797, 1085, 1448
BfmI	CTRYAG	2 cut(s)	1141, 2013
BfoI	RGCGCY	2 cut(s)	263, 429
BfrI	CTTAAG	1 cut(s)	94
BfuAI	ACCTGC	3 cut(s)	917, 1070, 1741
BglII	AGATCT	1 cut(s)	1800
BisI	GCNGC	6 cut(s)	170, 209, 527, 1527, 1581, 1674
BlpI	GCTNAGC	1 cut(s)	1065
BlsI	GCNGC	6 cut(s)	171, 210, 528, 1528, 1582, 1675
BmcAI	AGTACT	1 cut(s)	406
Bme1390I	CCNGG	5 cut(s)	11, 599, 1250, 1414, 1415
Bme18I	GGWCC	1 cut(s)	1003
BmeT110I	CYCGRG	1 cut(s)	1413
BmgT120I	GGNCC	2 cut(s)	625, 1003
BmiI	GGNNCC	7 cut(s)	58, 84, 161, 341, 626, 1302, 1605
BmrFI	CCNGG	5 cut(s)	11, 599, 1250, 1414, 1415
BmrI	ACTGGG	1 cut(s)	306
BmsI	GCATC	8 cut(s)	550, 685, 740, 853, 876, 1179, 1272, 1410
BmuI	ACTGGG	1 cut(s)	306
BoxI	GACNNNNGTC	1 cut(s)	1170
BpiI	GAAGAC	2 cut(s)	902, 1170
BpmI	CTGGAG	1 cut(s)	1791
Bpu1102I	GCTNAGC	1 cut(s)	1065
BpuEI	CTTGAG	1 cut(s)	1181
BpuMI	CCSGG	4 cut(s)	11, 1250, 1414, 1415
Bsa29I	ATCGAT	2 cut(s)	446, 831
BsaAI	YACGTR	1 cut(s)	566
BsaBI	GATNNNNATC	1 cut(s)	109
BsaHI	GRCGYC	3 cut(s)	240, 1901, 2064
BsaJI	CCNNGG	6 cut(s)	252, 890, 1249, 1412, 1413, 1552
BsaWI	WCCGGW	3 cut(s)	660, 989, 1054
Bsc4I	CCNNNNNNNGG	3 cut(s)	726, 1813, 2086
Bse1I	ACTGG	4 cut(s)	136, 301, 1929, 2120
Bse21I	CCTNAGG	1 cut(s)	1857
Bse3DI	GCAATG	2 cut(s)	164, 921
Bse8I	GATNNNNATC	1 cut(s)	109
BseBI	CCWGG	1 cut(s)	599
BseCI	ATCGAT	2 cut(s)	446, 831
BseDI	CCNNGG	6 cut(s)	252, 890, 1249, 1412, 1413, 1552
BseGI	GGATG	8 cut(s)	555, 914, 1373, 1474, 1605, 1822, 1963, 2095
BseJI	GATNNNNATC	1 cut(s)	109
BseLI	CCNNNNNNNGG	3 cut(s)	726, 1813, 2086
BseMI	GCAATG	2 cut(s)	164, 921
BseMII	CTCAG	3 cut(s)	201, 881, 1848
BseNI	ACTGG	4 cut(s)	136, 301, 1929, 2120
BseRI	GAGGAG	1 cut(s)	1874
BseXI	GCAGC	4 cut(s)	513, 1513, 1592, 1660
BseYI	CCCAGC	3 cut(s)	698, 737, 1229
BsgI	GTGCAG	1 cut(s)	2000
Bsh1236I	CGCG	2 cut(s)	211, 416
BshFI	GGCC	5 cut(s)	627, 656, 1118, 1248, 1928
BshNI	GGYRCC	2 cut(s)	339, 1300
BshVI	ATCGAT	2 cut(s)	446, 831
BsiHKAI	GWGCWC	2 cut(s)	1569, 1635
BsiHKCI	CYCGRG	1 cut(s)	1413
BsiSI	CCGG	6 cut(s)	11, 661, 990, 1055, 1249, 1414
BslFI	GGGAC	2 cut(s)	1975, 2075
BslI	CCNNNNNNNGG	3 cut(s)	726, 1813, 2086
BsmAI	GTCTC	1 cut(s)	1995
BsmFI	GGGAC	2 cut(s)	1975, 2075
BsmI	GAATGC	2 cut(s)	1222, 1719
BsnI	GGCC	5 cut(s)	627, 656, 1118, 1248, 1928
BsoBI	CYCGRG	1 cut(s)	1413
Bsp1286I	GDGCHC	3 cut(s)	252, 1569, 1635
Bsp1407I	TGTACA	1 cut(s)	837
Bsp1720I	GCTNAGC	1 cut(s)	1065
Bsp19I	CCATGG	1 cut(s)	1552
BspACI	CCGC	9 cut(s)	19, 54, 100, 170, 209, 384, 398, 1990, 1998
BspANI	GGCC	5 cut(s)	627, 656, 1118, 1248, 1928
BspCNI	CTCAG	3 cut(s)	200, 880, 1849
BspDI	ATCGAT	2 cut(s)	446, 831
BspFNI	CGCG	2 cut(s)	211, 416
BspLI	GGNNCC	7 cut(s)	58, 84, 161, 341, 626, 1302, 1605
BspMAI	CTGCAG	1 cut(s)	2017
BspMI	ACCTGC	3 cut(s)	917, 1070, 1741
BspT107I	GGYRCC	2 cut(s)	339, 1300
BspTI	CTTAAG	1 cut(s)	94
BsrBI	CCGCTC	1 cut(s)	54
BsrDI	GCAATG	2 cut(s)	164, 921
BsrGI	TGTACA	1 cut(s)	837
BsrI	ACTGG	4 cut(s)	136, 301, 1929, 2120
BssECI	CCNNGG	6 cut(s)	252, 890, 1249, 1412, 1413, 1552
BssNI	GRCGYC	3 cut(s)	240, 1901, 2064
BssT1I	CCWWGG	2 cut(s)	252, 1552
Bst2UI	CCWGG	1 cut(s)	599
Bst4CI	ACNGT	6 cut(s)	131, 768, 904, 950, 1652, 1847
BstACI	GRCGYC	3 cut(s)	240, 1901, 2064
BstAFI	CTTAAG	1 cut(s)	94
BstAPI	GCANNNNNTGC	2 cut(s)	932, 1978
BstAUI	TGTACA	1 cut(s)	837
BstBAI	YACGTR	1 cut(s)	566
BstC8I	GCNNGC	5 cut(s)	1047, 1071, 1339, 1726, 2068
BstDEI	CTNAG	7 cut(s)	87, 187, 867, 1065, 1616, 1694, 1857
BstDSI	CCRYGG	2 cut(s)	890, 1552
BstF5I	GGATG	8 cut(s)	555, 914, 1373, 1474, 1605, 1822, 1963, 2095
BstFNI	CGCG	2 cut(s)	211, 416
BstH2I	RGCGCY	2 cut(s)	263, 429
BstHHI	GCGC	3 cut(s)	213, 262, 428
BstMAI	GTCTC	1 cut(s)	1995
BstNI	CCWGG	1 cut(s)	599
BstNSI	RCATGY	4 cut(s)	539, 982, 1064, 1728
BstPAI	GACNNNNGTC	1 cut(s)	1170
BstSCI	CCNGG	5 cut(s)	9, 597, 1248, 1412, 1413
BstSFI	CTRYAG	2 cut(s)	1141, 2013
BstUI	CGCG	2 cut(s)	211, 416
BstV1I	GCAGC	4 cut(s)	513, 1513, 1592, 1660
BstV2I	GAAGAC	2 cut(s)	902, 1170
BstX2I	RGATCY	5 cut(s)	6, 45, 56, 82, 1800
BstYI	RGATCY	5 cut(s)	6, 45, 56, 82, 1800
Bsu15I	ATCGAT	2 cut(s)	446, 831
Bsu36I	CCTNAGG	1 cut(s)	1857
BsuRI	GGCC	5 cut(s)	627, 656, 1118, 1248, 1928
BsuTUI	ATCGAT	2 cut(s)	446, 831
BtgI	CCRYGG	2 cut(s)	890, 1552
BtgZI	GCGATG	3 cut(s)	1269, 1433, 1915
BtsCI	GGATG	8 cut(s)	555, 914, 1373, 1474, 1605, 1822, 1963, 2095
BtsI	GCAGTG	1 cut(s)	1967
BtsIMutI	CAGTG	4 cut(s)	195, 875, 955, 1967
BveI	ACCTGC	3 cut(s)	917, 1070, 1741
Cac8I	GCNNGC	5 cut(s)	1047, 1071, 1339, 1726, 2068
CaiI	CAGNNNCTG	1 cut(s)	202
CfoI	GCGC	3 cut(s)	213, 262, 428
Cfr13I	GGNCC	2 cut(s)	625, 1003
Cfr9I	CCCGGG	1 cut(s)	1413
ClaI	ATCGAT	2 cut(s)	446, 831
CseI	GACGC	4 cut(s)	422, 1175, 1502, 2072
CsiI	ACCWGGT	1 cut(s)	597
Csp6I	GTAC	6 cut(s)	127, 340, 405, 838, 970, 1301
CviQI	GTAC	6 cut(s)	127, 340, 405, 838, 970, 1301
DdeI	CTNAG	7 cut(s)	87, 187, 867, 1065, 1616, 1694, 1857
EaeI	YGGCCR	3 cut(s)	1116, 1246, 1926
EciI	GGCGGA	1 cut(s)	115
Ecl136II	GAGCTC	1 cut(s)	1567
Eco130I	CCWWGG	2 cut(s)	252, 1552
Eco147I	AGGCCT	1 cut(s)	656
Eco24I	GRGCYC	2 cut(s)	252, 1569
Eco47I	GGWCC	1 cut(s)	1003
Eco47III	AGCGCT	1 cut(s)	427
Eco53kI	GAGCTC	1 cut(s)	1567
Eco57I	CTGAAG	1 cut(s)	224
Eco81I	CCTNAGG	1 cut(s)	1857
Eco88I	CYCGRG	1 cut(s)	1413
EcoICRI	GAGCTC	1 cut(s)	1567
EcoO109I	RGGNCCY	1 cut(s)	625
EcoRII	CCWGG	1 cut(s)	597
EcoT14I	CCWWGG	2 cut(s)	252, 1552
EcoT22I	ATGCAT	2 cut(s)	537, 1425
EcoT38I	GRGCYC	2 cut(s)	252, 1569
ErhI	CCWWGG	2 cut(s)	252, 1552
FalI	AAGNNNNNCTT	2 cut(s)	1033, 1065
FaqI	GGGAC	2 cut(s)	1975, 2075
FauI	CCCGC	2 cut(s)	377, 391
FbaI	TGATCA	2 cut(s)	1023, 1944
Fnu4HI	GCNGC	6 cut(s)	170, 209, 527, 1527, 1581, 1674
FokI	GGATG	8 cut(s)	562, 921, 1380, 1481, 1612, 1829, 1970, 2102
FriOI	GRGCYC	2 cut(s)	252, 1569
Fsp4HI	GCNGC	6 cut(s)	170, 209, 527, 1527, 1581, 1674
FspBI	CTAG	3 cut(s)	797, 1085, 1448
GlaI	GCGC	3 cut(s)	212, 261, 427
GluI	GCNGC	6 cut(s)	170, 209, 527, 1527, 1581, 1674
GsaI	CCCAGC	3 cut(s)	702, 741, 1233
GsuI	CTGGAG	1 cut(s)	1791
HaeII	RGCGCY	2 cut(s)	263, 429
HaeIII	GGCC	5 cut(s)	627, 656, 1118, 1248, 1928
HapII	CCGG	6 cut(s)	11, 661, 990, 1055, 1249, 1414
HgaI	GACGC	4 cut(s)	422, 1175, 1502, 2072
HhaI	GCGC	3 cut(s)	213, 262, 428
Hin1I	GRCGYC	3 cut(s)	240, 1901, 2064
Hin6I	GCGC	3 cut(s)	211, 260, 426
HinP1I	GCGC	3 cut(s)	211, 260, 426
HincII	GTYRAC	1 cut(s)	1135
HindII	GTYRAC	1 cut(s)	1135
HindIII	AAGCTT	1 cut(s)	1473
HpaII	CCGG	6 cut(s)	11, 661, 990, 1055, 1249, 1414
HphI	GGTGA	3 cut(s)	433, 1044, 1979
Hpy166II	GTNNAC	1 cut(s)	1135
Hpy188III	TCNNGA	7 cut(s)	49, 316, 1198, 1289, 1454, 1770, 1877
Hpy8I	GTNNAC	1 cut(s)	1135
Hpy99I	CGWCG	5 cut(s)	232, 242, 245, 904, 1906
HpyAV	CCTTC	4 cut(s)	374, 667, 1286, 2037
HpyCH4III	ACNGT	6 cut(s)	131, 768, 904, 950, 1652, 1847
HpyCH4IV	ACGT	5 cut(s)	240, 565, 1203, 1363, 1901
HpyF3I	CTNAG	7 cut(s)	87, 187, 867, 1065, 1616, 1694, 1857
HpySE526I	ACGT	5 cut(s)	240, 565, 1203, 1363, 1901
Hsp92I	GRCGYC	3 cut(s)	240, 1901, 2064
HspAI	GCGC	3 cut(s)	211, 260, 426
KpnI	GGTACC	2 cut(s)	343, 1304
Ksp22I	TGATCA	2 cut(s)	1023, 1944
LmnI	GCTCC	4 cut(s)	193, 199, 257, 1501
Lsp1109I	GCAGC	4 cut(s)	513, 1513, 1592, 1660
LweI	GCATC	8 cut(s)	550, 685, 740, 853, 876, 1179, 1272, 1410
MabI	ACCWGGT	1 cut(s)	597
MaeI	CTAG	3 cut(s)	797, 1085, 1448
MaeII	ACGT	5 cut(s)	240, 565, 1203, 1363, 1901
MaeIII	GTNAC	6 cut(s)	702, 944, 1312, 1847, 1985, 2035
MbiI	CCGCTC	1 cut(s)	54
MboII	GAAGA	7 cut(s)	281, 799, 872, 907, 1167, 1175, 1291
MflI	RGATCY	5 cut(s)	6, 45, 56, 82, 1800
MhlI	GDGCHC	3 cut(s)	252, 1569, 1635
MlsI	TGGCCA	2 cut(s)	1118, 1928
MluI	ACGCGT	1 cut(s)	414
MluNI	TGGCCA	2 cut(s)	1118, 1928
MlyI	GAGTC	4 cut(s)	741, 1557, 1684, 1993
MmeI	TCCRAC	2 cut(s)	18, 480
Mox20I	TGGCCA	2 cut(s)	1118, 1928
Mph1103I	ATGCAT	2 cut(s)	537, 1425
MscI	TGGCCA	2 cut(s)	1118, 1928
MseI	TTAA	9 cut(s)	95, 285, 459, 996, 1223, 1637, 1743, 2019, 2024
Msp20I	TGGCCA	2 cut(s)	1118, 1928
MspCI	CTTAAG	1 cut(s)	94
MspI	CCGG	6 cut(s)	11, 661, 990, 1055, 1249, 1414
MspR9I	CCNGG	5 cut(s)	11, 599, 1250, 1414, 1415
Mva1269I	GAATGC	2 cut(s)	1222, 1719
MvaI	CCWGG	1 cut(s)	599
MvnI	CGCG	2 cut(s)	211, 416
NciI	CCSGG	4 cut(s)	11, 1250, 1414, 1415
NcoI	CCATGG	1 cut(s)	1552
NlaIV	GGNNCC	7 cut(s)	58, 84, 161, 341, 626, 1302, 1605
NmuCI	GTSAC	2 cut(s)	702, 1985
NsiI	ATGCAT	2 cut(s)	537, 1425
NspI	RCATGY	4 cut(s)	539, 982, 1064, 1728
PaeI	GCATGC	1 cut(s)	1728
PaqCI	CACCTGC	1 cut(s)	1741
PceI	AGGCCT	1 cut(s)	656
PctI	GAATGC	2 cut(s)	1222, 1719
PfeI	GAWTC	6 cut(s)	635, 724, 986, 1032, 1382, 2073
PflMI	CCANNNNNTGG	1 cut(s)	1813
PkrI	GCNGC	6 cut(s)	171, 210, 528, 1528, 1582, 1675
PleI	GAGTC	4 cut(s)	741, 1556, 1684, 1992
PpsI	GAGTC	4 cut(s)	741, 1556, 1684, 1992
Ppu21I	YACGTR	1 cut(s)	566
PshAI	GACNNNNGTC	1 cut(s)	1170
Psp124BI	GAGCTC	1 cut(s)	1569
Psp6I	CCWGG	1 cut(s)	597
PspFI	CCCAGC	3 cut(s)	698, 737, 1229
PspGI	CCWGG	1 cut(s)	597
PspN4I	GGNNCC	7 cut(s)	58, 84, 161, 341, 626, 1302, 1605
PspPI	GGNCC	2 cut(s)	625, 1003
PstI	CTGCAG	1 cut(s)	2017
PstNI	CAGNNNCTG	1 cut(s)	202
PsuI	RGATCY	5 cut(s)	6, 45, 56, 82, 1800
RsaI	GTAC	6 cut(s)	128, 341, 406, 839, 971, 1302
RsaNI	GTAC	6 cut(s)	127, 340, 405, 838, 970, 1301
SacI	GAGCTC	1 cut(s)	1569
SaqAI	TTAA	9 cut(s)	95, 285, 459, 996, 1223, 1637, 1743, 2019, 2024
SatI	GCNGC	6 cut(s)	170, 209, 527, 1527, 1581, 1674
Sau96I	GGNCC	2 cut(s)	625, 1003
ScaI	AGTACT	1 cut(s)	406
SchI	GAGTC	4 cut(s)	741, 1557, 1684, 1993
ScrFI	CCNGG	5 cut(s)	11, 599, 1250, 1414, 1415
SduI	GDGCHC	3 cut(s)	252, 1569, 1635
SexAI	ACCWGGT	1 cut(s)	597
SfaNI	GCATC	8 cut(s)	550, 685, 740, 853, 876, 1179, 1272, 1410
SfcI	CTRYAG	2 cut(s)	1141, 2013
SinI	GGWCC	1 cut(s)	1003
SmaI	CCCGGG	1 cut(s)	1415
SmlI	CTYRAG	2 cut(s)	94, 1196
SmoI	CTYRAG	2 cut(s)	94, 1196
SphI	GCATGC	1 cut(s)	1728
SseBI	AGGCCT	1 cut(s)	656
SsiI	CCGC	9 cut(s)	19, 54, 100, 170, 209, 384, 398, 1990, 1998
SspMI	CTAG	3 cut(s)	797, 1085, 1448
SstI	GAGCTC	1 cut(s)	1569
StuI	AGGCCT	1 cut(s)	656
StyD4I	CCNGG	5 cut(s)	9, 597, 1248, 1412, 1413
StyI	CCWWGG	2 cut(s)	252, 1552
TaaI	ACNGT	6 cut(s)	131, 768, 904, 950, 1652, 1847
TaiI	ACGT	5 cut(s)	243, 568, 1206, 1366, 1904
TatI	WGTACW	3 cut(s)	404, 837, 969
TauI	GCSGC	2 cut(s)	172, 211
TfiI	GAWTC	6 cut(s)	635, 724, 986, 1032, 1382, 2073
Tru1I	TTAA	9 cut(s)	95, 285, 459, 996, 1223, 1637, 1743, 2019, 2024
Tru9I	TTAA	9 cut(s)	95, 285, 459, 996, 1223, 1637, 1743, 2019, 2024
TscAI	CASTG	4 cut(s)	195, 875, 955, 1974
TseFI	GTSAC	2 cut(s)	702, 1985
TseI	GCWGC	4 cut(s)	526, 1526, 1580, 1673
Tsp45I	GTSAC	2 cut(s)	702, 1985
TspDTI	ATGAA	7 cut(s)	405, 594, 637, 761, 929, 1485, 1902
TspGWI	ACGGA	3 cut(s)	721, 907, 1714
TspMI	CCCGGG	1 cut(s)	1413
TspRI	CASTG	4 cut(s)	195, 875, 955, 1974
Van91I	CCANNNNNTGG	1 cut(s)	1813
Vha464I	CTTAAG	1 cut(s)	94
VpaK11BI	GGWCC	1 cut(s)	1003
XapI	RAATTY	3 cut(s)	643, 1558, 1574
XceI	RCATGY	4 cut(s)	539, 982, 1064, 1728
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1728, 2087
XmaI	CCCGGG	1 cut(s)	1413
XspI	CTAG	3 cut(s)	797, 1085, 1448
ZraI	GACGTC	2 cut(s)	241, 1902
ZrmI	AGTACT	1 cut(s)	406
Zsp2I	ATGCAT	2 cut(s)	537, 1425

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.