Rroxscaffold_7G00200650

Pentatricopeptide repeat-containing protein At5g50390

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005465
Physical Location & Seq
Reverse (-)
48004939 .. 48007756
2818 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00200650.1

Sequence Viewer

Length: 2127 bp
ATGGAGGTTCCTCTGTTAAGGTACCAAGGCCTAGTGTTGGACCAGATTCAATGTAGCATTAGCTTCCCTCTGTCATTCTCAGATTCCAGGTTTTCCAAGCGGAGATCTTTGTTTTCTGGTTACACTTTTTCCCTTAATAGACCAAAATGGAGGAACCCATTTTCCCAAATTAGGTGTTGTTCTTATGTGGAGCAACAGCTGAAGCCCCGGCCCAGGCCCATACCCGCCAAAATTGAGGTTGAGGAGGAACCAAAGGTCGCACCTTTGGAACCCGAAGAGGCCCAGATTGCAGTACCCAGTTCTGGGATTTGTAGTCAGATTGAGAAGTTGGTTTTGTATAAGAGGTATAGGGAAGCACTTGAAATGTTTGAGTTTTTGGAATCTAGAGGTGGGTATGAAGTGGGTGGTAGTACTTATGATGCTTTAGTGAGTGCTTGTATTAGCTTGAAATCGATTAGAGGAGTGAAGAGGGTGTTTGGTTATATGATTGGTAATGGGTTTGAGTTGGATCAGTACATGAGGAACAGGGTTTTGCTTATGCATGTGAGATGTGGGATGATGATTGATGCACGCCACCTGTTTGAGGAAATGCCTGAGAGAAATTCGGTTTCGTGGAACACGATAATTGGGGGTCTTGTGGATTGTGGAGAGTTCATGGAGGCGTTCGGGTTGTTTTTGGATATGTGGGAGGAGTTTTCAGATGGGGAGGCGAGGATATTTGCCACAATGATTCGGGCTTCGGCTGGGTTGGGACTCATTTCTGCAGGGAGACAGTTTCATTCGTGTTGTGTGAAGATGGGTGTGGCGGCTGATATTTTCGTGTCTTGTGCGTTGATTGATATGTATAGCAAGTGTGGGAGCATTGATGACGCTCACTGTGTTTTTGATGAGATGCCGAAGAAGACAACTGTAGGATGGAATACCATTATAGCGGGTTATGCACTTCATGGTTATAGTGAGGAGGCTTTGAGTATGTACTATGATATGCGTGATTCTGGTGTGCCGATGGACCATTTTACGTTTTCCATGATCATAAGAACATGTGCAAGGTTGGCGTCGTTAGAACATGCAAAGCAAGCACATGCAGGTCTAGTTCGTCATGGTTTTGGGTTGGATATAGTGGCAAACACATCGCTCGTGGACTTCTATAGCAAATGGGGGAGGATAGAAGATGCTCGTCATGTTTTTGATCAGATGCCTAACAAGAATGTCATATCTTGGAATGCCTTGATTGCTGGGTATGGTAACCATGGTCGGGGTGATGAGGCTGTTGAGATGTTTGAGAAGATGCTTCAGGAAGGAATGGTACCCAACCATGTTACTTTTCTTGCTGTCTTGTCTGCTTGCAGTCGTTCAGGTTTATCAGAACTTGGATGGGAGATTTTTGAATCAATGAGCATGGATTACAAGATTAAGCCCCGTGCTATGCATTATGCTTGTATGATTGAATTGCTAGGACAAGAGGGCAATTTAGATGAAGCGTTTGCACTGATAAGAACTGCTCCCTTAAAGCCTACAGCAAACATGTGGGCTGCTCTGCTGACAGCTTGTCGAATCCATGAGAATTTAGAGCTTGGCAAAATTGCAGCTGAAGAGCTCTATGGGATGGAGCCTGAGAAGCTAAGTAATTATGTTGTGCTCCTGAATATATATAACAGTTGTGGAAAGTTGAAGGAAGCTGCTGCTGTTGTTCAGACTCTAAGAAGAAAGGGTTTGAGAATGCTTCCGGCATGCAGTTGGATTGAAATTAAGAAACAGGTACATATTTTTCATTCCGGAGATGAAAGGCATGCCCAAACAAGAGAGATTTACGAGAAAGTGGACGAATTGATGGTCAGGATTAGGAGACGTGGTTACATCCCTGACAAGAAGCATCTACTTCCTGATGTGGATGAACACGAGCGCATCTCAGCTTACCACAGTGAGAAACTGGCTATAGCTTACGGCTTGATAAGTACGTCAGATGGAACGCCACTGCAAATTGTGCAAGGCCATCGCATTTGCGGAGACTGCCATACCGCAATCAAGTTAATAGCTAAGGTGACTGAACGGGAAATTGTAGTGAGGGACGCCAGCAGATTCCACCACTTCAAGAATGGGAGTTGTTCTTGTGGGAATTACTGGTGA

Protein Analysis

708

Amino Acids

80.12

Weight (kDa)

6.49

Isoelectric Point (pI)

46.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 302 - 350 1.1e-11 PPR repeat family
PPR PF01535 306 - 334 2.5e-06 PPR repeat
PPR_2 PF13041 402 - 450 6.4e-13 PPR repeat family
PPR_1 PF12854 403 - 430 6.2e-06 PPR repeat
PPR_3 PF13812 403 - 449 9.4e-06 Pentatricopeptide repeat domain
PPR PF01535 405 - 434 7.5e-09 PPR repeat
PPR_long PF17177 422 - 569 7.5e-06 Pentacotripeptide-repeat region of PRORP
E_motif PF20431 520 - 582 2.5e-11 E motif
DYW_deaminase PF14432 618 - 708 1.6e-36 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1076
Acc65I GGTACC 2 cut(s) 21, 1306
AccB1I GGYRCC 2 cut(s) 21, 1306
AccIII TCCGGA 1 cut(s) 1775
AciI CCGC 6 cut(s) 100, 225, 806, 932, 2004, 2019
AclWI GGATC 1 cut(s) 516
AcsI RAATTY 2 cut(s) 601, 1564
AcuI CTGAAG 3 cut(s) 221, 1277, 1611
AcyI GRCGYC 2 cut(s) 1055, 2070
AfaI GTAC 8 cut(s) 23, 294, 412, 515, 977, 1308, 1761, 1957
AfiI CCNNNNNNNGG 7 cut(s) 37, 171, 213, 302, 303, 583, 1255
AflIII ACRYGT 2 cut(s) 1040, 1524
AgsI TTSAA 8 cut(s) 50, 362, 448, 1388, 1448, 1672, 1745, 2092
AhdI GACNNNNNGTC 1 cut(s) 1548
AjiI CACGTC 1 cut(s) 1850
AjnI CCWGG 2 cut(s) 86, 212
AloI GAACNNNNNNTCC 2 cut(s) 146, 178
Alw21I GWGCWC 2 cut(s) 1599, 1641
Alw26I GTCTC 3 cut(s) 763, 1840, 2001
AlwI GGATC 1 cut(s) 516
Aor13HI TCCGGA 1 cut(s) 1775
AoxI GGCC 5 cut(s) 28, 209, 215, 279, 1990
ApeKI GCWGC 4 cut(s) 1532, 1586, 1679, 1682
ApoI RAATTY 2 cut(s) 601, 1564
ArsI GACNNNNNNTTYG 2 cut(s) 1818, 1850
Asp718I GGTACC 2 cut(s) 21, 1306
AspLEI GCGC 1 cut(s) 1905
AspS9I GGNCC 5 cut(s) 40, 210, 216, 280, 1009
AsuC2I CCSGG 1 cut(s) 208
AsuHPI GGTGA 2 cut(s) 1271, 2053
AvaII GGWCC 2 cut(s) 40, 1009
BanI GGYRCC 2 cut(s) 21, 1306
BanII GRGCYC 1 cut(s) 1599
BarI GAAGNNNNNNTAC 2 cut(s) 1290, 1322
BauI CACGAG 2 cut(s) 1136, 1898
BbsI GAAGAC 1 cut(s) 908
Bbv12I GWGCWC 2 cut(s) 1599, 1641
BbvI GCAGC 4 cut(s) 1519, 1598, 1666, 1669
BccI CCATC 9 cut(s) 695, 790, 909, 1000, 1368, 1600, 1825, 1958, 2001
BceAI ACGGC 1 cut(s) 1960
BcgI CGANNNNNNTGC 4 cut(s) 1113, 1147, 1976, 2010
BciT130I CCWGG 2 cut(s) 88, 214
BclI TGATCA 2 cut(s) 1029, 1189
BcnI CCSGG 1 cut(s) 208
BcoDI GTCTC 3 cut(s) 763, 1840, 2001
BfaI CTAG 4 cut(s) 32, 384, 1091, 1454
BfmI CTRYAG 5 cut(s) 762, 909, 1147, 1515, 1935
BfuAI ACCTGC 1 cut(s) 1076
BglII AGATCT 1 cut(s) 104
BisI GCNGC 5 cut(s) 807, 1533, 1587, 1680, 1683
BlsI GCNGC 5 cut(s) 808, 1534, 1588, 1681, 1684
BmcAI AGTACT 1 cut(s) 412
Bme1390I CCNGG 3 cut(s) 88, 208, 214
Bme18I GGWCC 2 cut(s) 40, 1009
BmeRI GACNNNNNGTC 1 cut(s) 1548
BmgBI CACGTC 1 cut(s) 1850
BmgT120I GGNCC 5 cut(s) 40, 210, 216, 280, 1009
BmiI GGNNCC 7 cut(s) 9, 23, 155, 249, 270, 1308, 1611
BmrFI CCNGG 3 cut(s) 88, 208, 214
BmrI ACTGGG 1 cut(s) 291
BmsI GCATC 8 cut(s) 409, 556, 882, 1162, 1185, 1278, 1882, 1914
BmuI ACTGGG 1 cut(s) 291
BpiI GAAGAC 1 cut(s) 908
BplI GAGNNNNNCTC 2 cut(s) 1892, 1924
Bpu10I CCTNAGC 1 cut(s) 2037
BpuMI CCSGG 1 cut(s) 208
Bsa29I ATCGAT 1 cut(s) 452
BsaHI GRCGYC 2 cut(s) 1055, 2070
BsaJI CCNNGG 4 cut(s) 25, 206, 212, 1249
BsaWI WCCGGW 1 cut(s) 1775
BsaXI ACNNNNNCTCC 2 cut(s) 1837, 1867
Bsc4I CCNNNNNNNGG 7 cut(s) 37, 171, 213, 302, 303, 583, 1255
Bse1I ACTGG 3 cut(s) 297, 1935, 2126
BseAI TCCGGA 1 cut(s) 1775
BseBI CCWGG 2 cut(s) 88, 214
BseCI ATCGAT 1 cut(s) 452
BseDI CCNNGG 4 cut(s) 25, 206, 212, 1249
BseGI GGATG 6 cut(s) 561, 920, 1379, 1611, 1857, 1897
BseLI CCNNNNNNNGG 7 cut(s) 37, 171, 213, 302, 303, 583, 1255
BseMII CTCAG 4 cut(s) 93, 585, 1605, 1923
BseNI ACTGG 3 cut(s) 297, 1935, 2126
BseRI GAGGAG 4 cut(s) 257, 474, 704, 974
BseXI GCAGC 4 cut(s) 1519, 1598, 1666, 1669
BseYI CCCAGC 2 cut(s) 743, 1235
BshFI GGCC 5 cut(s) 30, 211, 217, 281, 1992
BshNI GGYRCC 2 cut(s) 21, 1306
BshVI ATCGAT 1 cut(s) 452
BsiHKAI GWGCWC 2 cut(s) 1599, 1641
BsiSI CCGG 3 cut(s) 208, 1727, 1776
BslFI GGGAC 2 cut(s) 765, 2081
BslI CCNNNNNNNGG 7 cut(s) 37, 171, 213, 302, 303, 583, 1255
BsmAI GTCTC 3 cut(s) 763, 1840, 2001
BsmBI CGTCTC 1 cut(s) 1840
BsmFI GGGAC 2 cut(s) 765, 2081
BsmI GAATGC 2 cut(s) 1228, 1725
BsnI GGCC 5 cut(s) 30, 211, 217, 281, 1992
Bsp1286I GDGCHC 2 cut(s) 1599, 1641
Bsp13I TCCGGA 1 cut(s) 1775
Bsp143I GATC 4 cut(s) 104, 508, 1029, 1189
Bsp19I CCATGG 1 cut(s) 1249
BspACI CCGC 6 cut(s) 100, 225, 806, 932, 2004, 2019
BspANI GGCC 5 cut(s) 30, 211, 217, 281, 1992
BspCNI CTCAG 4 cut(s) 92, 586, 1606, 1922
BspDI ATCGAT 1 cut(s) 452
BspEI TCCGGA 1 cut(s) 1775
BspLI GGNNCC 7 cut(s) 9, 23, 155, 249, 270, 1308, 1611
BspMAI CTGCAG 1 cut(s) 766
BspMI ACCTGC 1 cut(s) 1076
BspPI GGATC 1 cut(s) 516
BspQI GCTCTTC 1 cut(s) 1587
BspT107I GGYRCC 2 cut(s) 21, 1306
BsrI ACTGG 3 cut(s) 297, 1935, 2126
BssECI CCNNGG 4 cut(s) 25, 206, 212, 1249
BssMI GATC 4 cut(s) 104, 508, 1029, 1189
BssNI GRCGYC 2 cut(s) 1055, 2070
BssSI CACGAG 2 cut(s) 1136, 1898
BssT1I CCWWGG 2 cut(s) 25, 1249
Bst2BI CACGAG 2 cut(s) 1136, 1898
Bst2UI CCWGG 2 cut(s) 88, 214
Bst4CI ACNGT 5 cut(s) 774, 878, 910, 1658, 1922
Bst6I CTCTTC 3 cut(s) 270, 461, 1587
BstACI GRCGYC 2 cut(s) 1055, 2070
BstAPI GCANNNNNTGC 1 cut(s) 1984
BstC8I GCNNGC 6 cut(s) 571, 1077, 1345, 1732, 1791, 2074
BstDEI CTNAG 7 cut(s) 79, 594, 1614, 1622, 1700, 1909, 2037
BstDSI CCRYGG 1 cut(s) 1249
BstEII GGTNACC 1 cut(s) 1244
BstENI CCTNNNNNAGG 1 cut(s) 581
BstF5I GGATG 6 cut(s) 561, 920, 1379, 1611, 1857, 1897
BstHHI GCGC 1 cut(s) 1905
BstKTI GATC 4 cut(s) 107, 511, 1032, 1192
BstMAI GTCTC 3 cut(s) 763, 1840, 2001
BstMBI GATC 4 cut(s) 104, 508, 1029, 1189
BstMWI GCNNNNNNNGC 8 cut(s) 287, 938, 1052, 1076, 1232, 1618, 1984, 2010
BstNI CCWGG 2 cut(s) 88, 214
BstNSI RCATGY 7 cut(s) 545, 1044, 1070, 1085, 1528, 1734, 1793
BstPI GGTNACC 1 cut(s) 1244
BstSCI CCNGG 3 cut(s) 86, 206, 212
BstSFI CTRYAG 5 cut(s) 762, 909, 1147, 1515, 1935
BstV1I GCAGC 4 cut(s) 1519, 1598, 1666, 1669
BstV2I GAAGAC 1 cut(s) 908
BstX2I RGATCY 1 cut(s) 104
BstYI RGATCY 1 cut(s) 104
Bsu15I ATCGAT 1 cut(s) 452
BsuRI GGCC 5 cut(s) 30, 211, 217, 281, 1992
BsuTUI ATCGAT 1 cut(s) 452
BtgI CCRYGG 1 cut(s) 1249
BtgZI GCGATG 2 cut(s) 1116, 1979
BtrI CACGTC 1 cut(s) 1850
BtsCI GGATG 6 cut(s) 561, 920, 1379, 1611, 1857, 1897
BtsI GCAGTG 1 cut(s) 1973
BtsIMutI CAGTG 4 cut(s) 874, 1487, 1927, 1973
BveI ACCTGC 1 cut(s) 1076
Cac8I GCNNGC 6 cut(s) 571, 1077, 1345, 1732, 1791, 2074
CfoI GCGC 1 cut(s) 1905
Cfr13I GGNCC 5 cut(s) 40, 210, 216, 280, 1009
ClaI ATCGAT 1 cut(s) 452
CseI GACGC 3 cut(s) 878, 1044, 2078
Csp6I GTAC 8 cut(s) 22, 293, 411, 514, 976, 1307, 1760, 1956
CviQI GTAC 8 cut(s) 22, 293, 411, 514, 976, 1307, 1760, 1956
DdeI CTNAG 7 cut(s) 79, 594, 1614, 1622, 1700, 1909, 2037
DpnI GATC 4 cut(s) 106, 510, 1031, 1191
DpnII GATC 4 cut(s) 104, 508, 1029, 1189
DriI GACNNNNNGTC 1 cut(s) 1548
Eam1104I CTCTTC 3 cut(s) 270, 461, 1587
Eam1105I GACNNNNNGTC 1 cut(s) 1548
EarI CTCTTC 3 cut(s) 270, 461, 1587
Ecl136II GAGCTC 1 cut(s) 1597
Eco130I CCWWGG 2 cut(s) 25, 1249
Eco147I AGGCCT 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 1599
Eco47I GGWCC 2 cut(s) 40, 1009
Eco53kI GAGCTC 1 cut(s) 1597
Eco57I CTGAAG 3 cut(s) 221, 1277, 1611
Eco91I GGTNACC 1 cut(s) 1244
EcoICRI GAGCTC 1 cut(s) 1597
EcoNI CCTNNNNNAGG 1 cut(s) 581
EcoO65I GGTNACC 1 cut(s) 1244
EcoRII CCWGG 2 cut(s) 86, 212
EcoT14I CCWWGG 2 cut(s) 25, 1249
EcoT22I ATGCAT 2 cut(s) 543, 1431
EcoT38I GRGCYC 1 cut(s) 1599
ErhI CCWWGG 2 cut(s) 25, 1249
Esp3I CGTCTC 1 cut(s) 1840
FaqI GGGAC 2 cut(s) 765, 2081
FauI CCCGC 2 cut(s) 232, 925
FbaI TGATCA 2 cut(s) 1029, 1189
Fnu4HI GCNGC 5 cut(s) 807, 1533, 1587, 1680, 1683
FokI GGATG 6 cut(s) 568, 927, 1386, 1618, 1844, 1904
FriOI GRGCYC 1 cut(s) 1599
Fsp4HI GCNGC 5 cut(s) 807, 1533, 1587, 1680, 1683
FspBI CTAG 4 cut(s) 32, 384, 1091, 1454
GlaI GCGC 1 cut(s) 1904
GluI GCNGC 5 cut(s) 807, 1533, 1587, 1680, 1683
GsaI CCCAGC 2 cut(s) 747, 1239
HaeIII GGCC 5 cut(s) 30, 211, 217, 281, 1992
HapII CCGG 3 cut(s) 208, 1727, 1776
HgaI GACGC 3 cut(s) 878, 1044, 2078
HhaI GCGC 1 cut(s) 1905
Hin1I GRCGYC 2 cut(s) 1055, 2070
Hin6I GCGC 1 cut(s) 1903
HinP1I GCGC 1 cut(s) 1903
HpaII CCGG 3 cut(s) 208, 1727, 1776
HphI GGTGA 2 cut(s) 1271, 2053
Hpy166II GTNNAC 2 cut(s) 1141, 1822
Hpy188I TCNGA 7 cut(s) 82, 318, 700, 1194, 1366, 1695, 1963
Hpy188III TCNNGA 7 cut(s) 384, 1295, 1642, 1776, 1837, 1883, 2092
Hpy8I GTNNAC 2 cut(s) 1141, 1822
Hpy99I CGWCG 1 cut(s) 1060
HpyAV CCTTC 2 cut(s) 1292, 1666
HpyCH4III ACNGT 5 cut(s) 774, 878, 910, 1658, 1922
HpyCH4IV ACGT 3 cut(s) 1019, 1849, 1958
HpyF10VI GCNNNNNNNGC 8 cut(s) 287, 938, 1052, 1076, 1232, 1618, 1984, 2010
HpyF3I CTNAG 7 cut(s) 79, 594, 1614, 1622, 1700, 1909, 2037
HpySE526I ACGT 3 cut(s) 1019, 1849, 1958
Hsp92I GRCGYC 2 cut(s) 1055, 2070
HspAI GCGC 1 cut(s) 1903
Kpn2I TCCGGA 1 cut(s) 1775
KpnI GGTACC 2 cut(s) 25, 1310
Ksp22I TGATCA 2 cut(s) 1029, 1189
Kzo9I GATC 4 cut(s) 104, 508, 1029, 1189
LguI GCTCTTC 1 cut(s) 1587
LmnI GCTCC 5 cut(s) 190, 858, 1507, 1609, 1644
Lsp1109I GCAGC 4 cut(s) 1519, 1598, 1666, 1669
LweI GCATC 8 cut(s) 409, 556, 882, 1162, 1185, 1278, 1882, 1914
MaeI CTAG 4 cut(s) 32, 384, 1091, 1454
MaeII ACGT 3 cut(s) 1019, 1849, 1958
MaeIII GTNAC 5 cut(s) 119, 1244, 1318, 1853, 2041
MalI GATC 4 cut(s) 106, 510, 1031, 1191
MboI GATC 4 cut(s) 104, 508, 1029, 1189
MboII GAAGA 9 cut(s) 287, 478, 805, 910, 913, 1181, 1297, 1604, 1716
MflI RGATCY 1 cut(s) 104
MhlI GDGCHC 2 cut(s) 1599, 1641
MlyI GAGTC 2 cut(s) 747, 1690
MmeI TCCRAC 4 cut(s) 18, 486, 1092, 1718
Mph1103I ATGCAT 2 cut(s) 543, 1431
MroI TCCGGA 1 cut(s) 1775
MseI TTAA 6 cut(s) 17, 135, 1413, 1508, 1749, 2030
MspA1I CMGCKG 2 cut(s) 199, 1589
MspI CCGG 3 cut(s) 208, 1727, 1776
MspR9I CCNGG 3 cut(s) 88, 208, 214
Mva1269I GAATGC 2 cut(s) 1228, 1725
MvaI CCWGG 2 cut(s) 88, 214
MwoI GCNNNNNNNGC 8 cut(s) 287, 938, 1052, 1076, 1232, 1618, 1984, 2010
NciI CCSGG 1 cut(s) 208
NcoI CCATGG 1 cut(s) 1249
NdeII GATC 4 cut(s) 104, 508, 1029, 1189
NlaIV GGNNCC 7 cut(s) 9, 23, 155, 249, 270, 1308, 1611
NmuCI GTSAC 1 cut(s) 2041
NsiI ATGCAT 2 cut(s) 543, 1431
NspI RCATGY 7 cut(s) 545, 1044, 1070, 1085, 1528, 1734, 1793
PaeI GCATGC 2 cut(s) 1734, 1793
PceI AGGCCT 1 cut(s) 30
PciI ACATGT 2 cut(s) 1040, 1524
PciSI GCTCTTC 1 cut(s) 1587
PcsI WCGNNNNNNNCGW 1 cut(s) 617
PctI GAATGC 2 cut(s) 1228, 1725
PfeI GAWTC 8 cut(s) 46, 83, 380, 730, 992, 1388, 1554, 2079
PkrI GCNGC 5 cut(s) 808, 1534, 1588, 1681, 1684
PleI GAGTC 2 cut(s) 747, 1690
PpsI GAGTC 2 cut(s) 747, 1690
PscI ACATGT 2 cut(s) 1040, 1524
Psp124BI GAGCTC 1 cut(s) 1599
Psp6I CCWGG 2 cut(s) 86, 212
PspEI GGTNACC 1 cut(s) 1244
PspFI CCCAGC 2 cut(s) 743, 1235
PspGI CCWGG 2 cut(s) 86, 212
PspN4I GGNNCC 7 cut(s) 9, 23, 155, 249, 270, 1308, 1611
PspPI GGNCC 5 cut(s) 40, 210, 216, 280, 1009
PstI CTGCAG 1 cut(s) 766
PsuI RGATCY 1 cut(s) 104
PvuII CAGCTG 2 cut(s) 199, 1589
RsaI GTAC 8 cut(s) 23, 294, 412, 515, 977, 1308, 1761, 1957
RsaNI GTAC 8 cut(s) 22, 293, 411, 514, 976, 1307, 1760, 1956
SacI GAGCTC 1 cut(s) 1599
SapI GCTCTTC 1 cut(s) 1587
SaqAI TTAA 6 cut(s) 17, 135, 1413, 1508, 1749, 2030
SatI GCNGC 5 cut(s) 807, 1533, 1587, 1680, 1683
Sau3AI GATC 4 cut(s) 104, 508, 1029, 1189
Sau96I GGNCC 5 cut(s) 40, 210, 216, 280, 1009
ScaI AGTACT 1 cut(s) 412
SchI GAGTC 2 cut(s) 747, 1690
ScrFI CCNGG 3 cut(s) 88, 208, 214
SduI GDGCHC 2 cut(s) 1599, 1641
SfaNI GCATC 8 cut(s) 409, 556, 882, 1162, 1185, 1278, 1882, 1914
SfcI CTRYAG 5 cut(s) 762, 909, 1147, 1515, 1935
SinI GGWCC 2 cut(s) 40, 1009
SphI GCATGC 2 cut(s) 1734, 1793
SseBI AGGCCT 1 cut(s) 30
SsiI CCGC 6 cut(s) 100, 225, 806, 932, 2004, 2019
SspMI CTAG 4 cut(s) 32, 384, 1091, 1454
SstI GAGCTC 1 cut(s) 1599
StuI AGGCCT 1 cut(s) 30
StyD4I CCNGG 3 cut(s) 86, 206, 212
StyI CCWWGG 2 cut(s) 25, 1249
TaaI ACNGT 5 cut(s) 774, 878, 910, 1658, 1922
TaiI ACGT 3 cut(s) 1022, 1852, 1961
TaqI TCGA 2 cut(s) 452, 1552
TatI WGTACW 3 cut(s) 410, 513, 975
TauI GCSGC 1 cut(s) 809
TfiI GAWTC 8 cut(s) 46, 83, 380, 730, 992, 1388, 1554, 2079
Tru1I TTAA 6 cut(s) 17, 135, 1413, 1508, 1749, 2030
Tru9I TTAA 6 cut(s) 17, 135, 1413, 1508, 1749, 2030
TscAI CASTG 4 cut(s) 881, 1494, 1927, 1980
TseFI GTSAC 1 cut(s) 2041
TseI GCWGC 4 cut(s) 1532, 1586, 1679, 1682
Tsp45I GTSAC 1 cut(s) 2041
TspDTI ATGAA 8 cut(s) 411, 643, 767, 935, 1491, 1760, 1797, 1908
TspRI CASTG 4 cut(s) 881, 1494, 1927, 1980
VpaK11BI GGWCC 2 cut(s) 40, 1009
XagI CCTNNNNNAGG 1 cut(s) 581
XapI RAATTY 2 cut(s) 601, 1564
XbaI TCTAGA 1 cut(s) 383
XceI RCATGY 7 cut(s) 545, 1044, 1070, 1085, 1528, 1734, 1793
XcmI CCANNNNNNNNNTGG 1 cut(s) 2093
XspI CTAG 4 cut(s) 32, 384, 1091, 1454
ZrmI AGTACT 1 cut(s) 412
Zsp2I ATGCAT 2 cut(s) 543, 1431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.