pycom111g04370

Pentatricopeptide repeat-containing protein At5g50390

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0005465
Physical Location & Seq
Reverse (-)
2629929 .. 2631012
1084 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g04370.1

Sequence Viewer

Length: 1035 bp
ATGACATGCGTGATTCTGGTGTTAAGATGGACCATTTCACATTTTCTATCGATCATAAGAATCTGTGCAAGATTGGCTTCACCGGAACATGCTAAGCAAGCTCATGCAGGTCTAGTTCGTCATGGTTTTGGGTTAGATATAGTGGCCAACACGTCACTCGTTGACTTCTATAGTAAATGGGGAAGAATAGAAGACGCCCGTCATGTTTTTGACCAGATGCCTCTCAAGAATGTAATATCTTGGAATGCTTTAATTGCTGGGTATGGTAATCATGGCCGGGGCAATGAGGCTGTTGAGATGTTTGAGAAGATGCTTCAGGAAGGAATGGTACCTAACCATGTTACATTTCTTGCTGTATTGTCTGCTTGCAGTCATTCTGGTTTATCAGAACGTGGATGGGAGACTTTTGAATCAATGAGCAGGGATCACAAGATTAAGCCCCGGGCGATGCATTATGCTTGTATGATTGAATTGCTAGGTCGAGAGGGGCTTTTGGATGAAGCCTATGCACTATTAATAGACGCTCCCTTTGATCCTACAGCAAATATGTGGGCTGCCCTGCTAACAGCTTGTCGAGTCCATGAGAATTTAGAGCTCGGAAAATTTGCTGCAGAAAAACTTTATGGGATGGAACCTGATAAGATAAGTAATTATATTGTGCTCTTAAATATATACAACAGTTCTGGGAAGTTGAGGGAGGCTGCTGGTGTCGTTCAGACTCTAAGACGGAAAGGTTTGAGAATGCTTCCAGCATGCACTTGGATCGAAATTAAAAAGCAGGTGCATATTTTTCGTTCTGGAGAAAAAAGCCACGCCGAAACAAGAGAGATCTACCAGAAAGTGGATGACTTGATAAGCAAGATTACAGAACACGGTTACACCCCTGAGGAGAAGTATCTACTTCCTGATGTCGATGAACATGAGCAGACGTCGTTTTACCACAGCGAGAAACTGGCCATCGCTTATGGTCTGATCAATACTTCGGATGGGACACCACTGCAGATTGTGCAGAGGTTACGGGAAGGGAAATCGTAG

Protein Analysis

345

Amino Acids

38.94

Weight (kDa)

6.6

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 76 - 124 3.3e-13 PPR repeat family
PPR_1 PF12854 77 - 104 1e-05 PPR repeat
PPR_3 PF13812 77 - 123 6.3e-06 Pentatricopeptide repeat domain
PPR PF01535 79 - 108 1.4e-08 PPR repeat
E_motif PF20431 194 - 256 9e-11 E motif
DYW_deaminase PF14432 292 - 340 1.9e-10 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 769
AatII GACGTC 1 cut(s) 932
Acc36I ACCTGC 2 cut(s) 98, 769
Acc65I GGTACC 1 cut(s) 328
AccB1I GGYRCC 1 cut(s) 328
AccB7I CCANNNNNTGG 1 cut(s) 841
AclWI GGATC 3 cut(s) 432, 527, 770
AcoI YGGCCR 3 cut(s) 144, 274, 954
AcsI RAATTY 2 cut(s) 586, 602
AcuI CTGAAG 1 cut(s) 299
AcyI GRCGYC 2 cut(s) 195, 929
AfaI GTAC 1 cut(s) 330
AfiI CCNNNNNNNGG 1 cut(s) 841
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 2 cut(s) 410, 470
AjiI CACGTC 1 cut(s) 153
AluBI AGCT 3 cut(s) 101, 569, 595
AluI AGCT 3 cut(s) 101, 569, 595
Alw21I GWGCWC 2 cut(s) 597, 663
Alw26I GTCTC 1 cut(s) 395
AlwI GGATC 3 cut(s) 432, 527, 770
Ama87I CYCGRG 1 cut(s) 441
AoxI GGCC 3 cut(s) 144, 274, 954
ApeKI GCWGC 3 cut(s) 554, 608, 701
ApoI RAATTY 2 cut(s) 586, 602
ArsI GACNNNNNNTTYG 2 cut(s) 512, 544
AseI ATTAAT 1 cut(s) 515
Asp718I GGTACC 1 cut(s) 328
AspS9I GGNCC 1 cut(s) 30
AsuC2I CCSGG 3 cut(s) 278, 442, 443
AsuHPI GGTGA 1 cut(s) 72
AvaI CYCGRG 1 cut(s) 441
AvaII GGWCC 1 cut(s) 30
AxyI CCTNAGG 1 cut(s) 885
BalI TGGCCA 2 cut(s) 146, 956
BanI GGYRCC 1 cut(s) 328
BanII GRGCYC 1 cut(s) 597
BarI GAAGNNNNNNTAC 2 cut(s) 312, 344
BbsI GAAGAC 1 cut(s) 198
Bbv12I GWGCWC 2 cut(s) 597, 663
BbvI GCAGC 3 cut(s) 541, 595, 688
BccI CCATC 5 cut(s) 21, 390, 622, 965, 980
BcgI CGANNNNNNTGC 4 cut(s) 745, 773, 779, 807
BclI TGATCA 1 cut(s) 972
BcnI CCSGG 3 cut(s) 278, 442, 443
BcoDI GTCTC 1 cut(s) 395
BfaI CTAG 2 cut(s) 113, 476
BfmI CTRYAG 4 cut(s) 169, 537, 609, 998
BfuAI ACCTGC 2 cut(s) 98, 769
BglII AGATCT 1 cut(s) 828
BisI GCNGC 3 cut(s) 555, 609, 702
BlpI GCTNAGC 1 cut(s) 93
BlsI GCNGC 3 cut(s) 556, 610, 703
Bme1390I CCNGG 3 cut(s) 278, 442, 443
Bme18I GGWCC 1 cut(s) 30
BmeT110I CYCGRG 1 cut(s) 441
BmgBI CACGTC 1 cut(s) 153
BmgT120I GGNCC 1 cut(s) 30
BmiI GGNNCC 2 cut(s) 330, 633
BmrFI CCNGG 3 cut(s) 278, 442, 443
BmsI GCATC 3 cut(s) 207, 300, 438
BoxI GACNNNNGTC 1 cut(s) 198
BpiI GAAGAC 1 cut(s) 198
BpmI CTGGAG 1 cut(s) 819
Bpu1102I GCTNAGC 1 cut(s) 93
BpuEI CTTGAG 1 cut(s) 209
BpuMI CCSGG 3 cut(s) 278, 442, 443
Bsa29I ATCGAT 1 cut(s) 50
BsaHI GRCGYC 2 cut(s) 195, 929
BsaJI CCNNGG 3 cut(s) 277, 440, 441
BsaWI WCCGGW 1 cut(s) 82
Bsc4I CCNNNNNNNGG 1 cut(s) 841
Bse1I ACTGG 1 cut(s) 957
Bse21I CCTNAGG 1 cut(s) 885
Bse3DI GCAATG 1 cut(s) 289
BseCI ATCGAT 1 cut(s) 50
BseDI CCNNGG 3 cut(s) 277, 440, 441
BseGI GGATG 5 cut(s) 401, 502, 633, 850, 991
BseLI CCNNNNNNNGG 1 cut(s) 841
BseMI GCAATG 1 cut(s) 289
BseMII CTCAG 1 cut(s) 876
BseNI ACTGG 1 cut(s) 957
BseRI GAGGAG 1 cut(s) 902
BseXI GCAGC 3 cut(s) 541, 595, 688
BseYI CCCAGC 1 cut(s) 257
BsgI GTGCAG 1 cut(s) 1028
BshFI GGCC 3 cut(s) 146, 276, 956
BshNI GGYRCC 1 cut(s) 328
BshVI ATCGAT 1 cut(s) 50
BsiHKAI GWGCWC 2 cut(s) 597, 663
BsiHKCI CYCGRG 1 cut(s) 441
BsiSI CCGG 3 cut(s) 83, 277, 442
BslFI GGGAC 1 cut(s) 1003
BslI CCNNNNNNNGG 1 cut(s) 841
BsmAI GTCTC 1 cut(s) 395
BsmFI GGGAC 1 cut(s) 1003
BsmI GAATGC 2 cut(s) 250, 747
BsnI GGCC 3 cut(s) 146, 276, 956
BsoBI CYCGRG 1 cut(s) 441
Bsp1286I GDGCHC 2 cut(s) 597, 663
Bsp143I GATC 6 cut(s) 51, 424, 532, 762, 828, 972
Bsp1720I GCTNAGC 1 cut(s) 93
BspANI GGCC 3 cut(s) 146, 276, 956
BspCNI CTCAG 1 cut(s) 877
BspDI ATCGAT 1 cut(s) 50
BspLI GGNNCC 2 cut(s) 330, 633
BspMAI CTGCAG 2 cut(s) 613, 1002
BspMI ACCTGC 2 cut(s) 98, 769
BspPI GGATC 3 cut(s) 432, 527, 770
BspT107I GGYRCC 1 cut(s) 328
BsrDI GCAATG 1 cut(s) 289
BsrI ACTGG 1 cut(s) 957
BssECI CCNNGG 3 cut(s) 277, 440, 441
BssMI GATC 6 cut(s) 51, 424, 532, 762, 828, 972
BssNI GRCGYC 2 cut(s) 195, 929
Bst4CI ACNGT 2 cut(s) 680, 875
BstACI GRCGYC 2 cut(s) 195, 929
BstAPI GCANNNNNTGC 1 cut(s) 1006
BstC8I GCNNGC 3 cut(s) 99, 367, 754
BstDEI CTNAG 3 cut(s) 93, 722, 885
BstF5I GGATG 5 cut(s) 401, 502, 633, 850, 991
BstKTI GATC 6 cut(s) 54, 427, 535, 765, 831, 975
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 6 cut(s) 51, 424, 532, 762, 828, 972
BstMWI GCNNNNNNNGC 4 cut(s) 74, 98, 254, 1006
BstNSI RCATGY 3 cut(s) 9, 92, 756
BstPAI GACNNNNGTC 1 cut(s) 198
BstSCI CCNGG 3 cut(s) 276, 440, 441
BstSFI CTRYAG 4 cut(s) 169, 537, 609, 998
BstV1I GCAGC 3 cut(s) 541, 595, 688
BstV2I GAAGAC 1 cut(s) 198
BstX2I RGATCY 1 cut(s) 828
BstYI RGATCY 1 cut(s) 828
Bsu15I ATCGAT 1 cut(s) 50
Bsu36I CCTNAGG 1 cut(s) 885
BsuRI GGCC 3 cut(s) 146, 276, 956
BsuTUI ATCGAT 1 cut(s) 50
BtgZI GCGATG 2 cut(s) 461, 943
BtrI CACGTC 1 cut(s) 153
BtsCI GGATG 5 cut(s) 401, 502, 633, 850, 991
BtsI GCAGTG 1 cut(s) 995
BtsIMutI CAGTG 1 cut(s) 995
BveI ACCTGC 2 cut(s) 98, 769
Cac8I GCNNGC 3 cut(s) 99, 367, 754
Cfr13I GGNCC 1 cut(s) 30
Cfr9I CCCGGG 1 cut(s) 441
ClaI ATCGAT 1 cut(s) 50
CseI GACGC 2 cut(s) 203, 530
Csp6I GTAC 1 cut(s) 329
CviQI GTAC 1 cut(s) 329
DdeI CTNAG 3 cut(s) 93, 722, 885
DpnI GATC 6 cut(s) 53, 426, 534, 764, 830, 974
DpnII GATC 6 cut(s) 51, 424, 532, 762, 828, 972
EaeI YGGCCR 3 cut(s) 144, 274, 954
Ecl136II GAGCTC 1 cut(s) 595
Eco24I GRGCYC 1 cut(s) 597
Eco47I GGWCC 1 cut(s) 30
Eco53kI GAGCTC 1 cut(s) 595
Eco57I CTGAAG 1 cut(s) 299
Eco81I CCTNAGG 1 cut(s) 885
Eco88I CYCGRG 1 cut(s) 441
EcoICRI GAGCTC 1 cut(s) 595
EcoT22I ATGCAT 1 cut(s) 453
EcoT38I GRGCYC 1 cut(s) 597
FalI AAGNNNNNCTT 2 cut(s) 61, 93
FaqI GGGAC 1 cut(s) 1003
FbaI TGATCA 1 cut(s) 972
Fnu4HI GCNGC 3 cut(s) 555, 609, 702
FokI GGATG 5 cut(s) 408, 509, 640, 857, 998
FriOI GRGCYC 1 cut(s) 597
Fsp4HI GCNGC 3 cut(s) 555, 609, 702
FspBI CTAG 2 cut(s) 113, 476
GluI GCNGC 3 cut(s) 555, 609, 702
GsaI CCCAGC 1 cut(s) 261
GsuI CTGGAG 1 cut(s) 819
HaeIII GGCC 3 cut(s) 146, 276, 956
HapII CCGG 3 cut(s) 83, 277, 442
HgaI GACGC 2 cut(s) 203, 530
Hin1I GRCGYC 2 cut(s) 195, 929
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 5 cut(s) 13, 60, 410, 576, 718
HpaII CCGG 3 cut(s) 83, 277, 442
HphI GGTGA 1 cut(s) 72
Hpy166II GTNNAC 1 cut(s) 163
Hpy188I TCNGA 5 cut(s) 388, 599, 717, 972, 985
Hpy188III TCNNGA 5 cut(s) 226, 317, 482, 798, 905
Hpy8I GTNNAC 1 cut(s) 163
Hpy99I CGWCG 1 cut(s) 934
HpyAV CCTTC 2 cut(s) 314, 1016
HpyCH4III ACNGT 2 cut(s) 680, 875
HpyCH4IV ACGT 3 cut(s) 152, 391, 929
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 98, 254, 1006
HpyF3I CTNAG 3 cut(s) 93, 722, 885
HpySE526I ACGT 3 cut(s) 152, 391, 929
Hsp92I GRCGYC 2 cut(s) 195, 929
KpnI GGTACC 1 cut(s) 332
Ksp22I TGATCA 1 cut(s) 972
Kzo9I GATC 6 cut(s) 51, 424, 532, 762, 828, 972
LmnI GCTCC 1 cut(s) 529
Lsp1109I GCAGC 3 cut(s) 541, 595, 688
LweI GCATC 3 cut(s) 207, 300, 438
MaeI CTAG 2 cut(s) 113, 476
MaeII ACGT 3 cut(s) 152, 391, 929
MaeIII GTNAC 4 cut(s) 153, 340, 875, 1014
MalI GATC 6 cut(s) 53, 426, 534, 764, 830, 974
MboI GATC 6 cut(s) 51, 424, 532, 762, 828, 972
MboII GAAGA 3 cut(s) 195, 203, 319
MflI RGATCY 1 cut(s) 828
MhlI GDGCHC 2 cut(s) 597, 663
MlsI TGGCCA 2 cut(s) 146, 956
MluCI AATT 6 cut(s) 252, 470, 586, 602, 649, 768
MluNI TGGCCA 2 cut(s) 146, 956
MlyI GAGTC 2 cut(s) 585, 712
MnlI CCTC 7 cut(s) 231, 280, 478, 687, 691, 880, 1005
Mox20I TGGCCA 2 cut(s) 146, 956
Mph1103I ATGCAT 1 cut(s) 453
MscI TGGCCA 2 cut(s) 146, 956
MseI TTAA 6 cut(s) 23, 251, 435, 515, 665, 771
Msp20I TGGCCA 2 cut(s) 146, 956
MspI CCGG 3 cut(s) 83, 277, 442
MspR9I CCNGG 3 cut(s) 278, 442, 443
Mva1269I GAATGC 2 cut(s) 250, 747
MwoI GCNNNNNNNGC 4 cut(s) 74, 98, 254, 1006
NciI CCSGG 3 cut(s) 278, 442, 443
NdeII GATC 6 cut(s) 51, 424, 532, 762, 828, 972
NlaIV GGNNCC 2 cut(s) 330, 633
NmuCI GTSAC 1 cut(s) 153
NsiI ATGCAT 1 cut(s) 453
NspI RCATGY 3 cut(s) 9, 92, 756
PaeI GCATGC 1 cut(s) 756
PaqCI CACCTGC 1 cut(s) 769
PctI GAATGC 2 cut(s) 250, 747
PfeI GAWTC 3 cut(s) 13, 60, 410
PflMI CCANNNNNTGG 1 cut(s) 841
PkrI GCNGC 3 cut(s) 556, 610, 703
PleI GAGTC 2 cut(s) 584, 712
PpsI GAGTC 2 cut(s) 584, 712
PshAI GACNNNNGTC 1 cut(s) 198
PshBI ATTAAT 1 cut(s) 515
Psp124BI GAGCTC 1 cut(s) 597
PspFI CCCAGC 1 cut(s) 257
PspN4I GGNNCC 2 cut(s) 330, 633
PspPI GGNCC 1 cut(s) 30
PstI CTGCAG 2 cut(s) 613, 1002
PsuI RGATCY 1 cut(s) 828
RsaI GTAC 1 cut(s) 330
RsaNI GTAC 1 cut(s) 329
SacI GAGCTC 1 cut(s) 597
SaqAI TTAA 6 cut(s) 23, 251, 435, 515, 665, 771
SatI GCNGC 3 cut(s) 555, 609, 702
Sau3AI GATC 6 cut(s) 51, 424, 532, 762, 828, 972
Sau96I GGNCC 1 cut(s) 30
SchI GAGTC 2 cut(s) 585, 712
ScrFI CCNGG 3 cut(s) 278, 442, 443
SduI GDGCHC 2 cut(s) 597, 663
SfaNI GCATC 3 cut(s) 207, 300, 438
SfcI CTRYAG 4 cut(s) 169, 537, 609, 998
SinI GGWCC 1 cut(s) 30
SmaI CCCGGG 1 cut(s) 443
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
SphI GCATGC 1 cut(s) 756
Sse9I AATT 6 cut(s) 252, 470, 586, 602, 649, 768
SspMI CTAG 2 cut(s) 113, 476
SstI GAGCTC 1 cut(s) 597
StyD4I CCNGG 3 cut(s) 276, 440, 441
TaaI ACNGT 2 cut(s) 680, 875
TaiI ACGT 3 cut(s) 155, 394, 932
TaqI TCGA 5 cut(s) 50, 481, 574, 765, 912
TasI AATT 6 cut(s) 252, 470, 586, 602, 649, 768
TfiI GAWTC 3 cut(s) 13, 60, 410
Tru1I TTAA 6 cut(s) 23, 251, 435, 515, 665, 771
Tru9I TTAA 6 cut(s) 23, 251, 435, 515, 665, 771
TscAI CASTG 1 cut(s) 1002
TseFI GTSAC 1 cut(s) 153
TseI GCWGC 3 cut(s) 554, 608, 701
Tsp45I GTSAC 1 cut(s) 153
TspDTI ATGAA 2 cut(s) 513, 930
TspGWI ACGGA 1 cut(s) 742
TspMI CCCGGG 1 cut(s) 441
TspRI CASTG 1 cut(s) 1002
Van91I CCANNNNNTGG 1 cut(s) 841
VpaK11BI GGWCC 1 cut(s) 30
VspI ATTAAT 1 cut(s) 515
XapI RAATTY 2 cut(s) 586, 602
XceI RCATGY 3 cut(s) 9, 92, 756
XcmI CCANNNNNNNNNTGG 1 cut(s) 756
XmaI CCCGGG 1 cut(s) 441
XspI CTAG 2 cut(s) 113, 476
ZraI GACGTC 1 cut(s) 930
Zsp2I ATGCAT 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.