Rh2BG614600

Pentatricopeptide repeat-containing protein At5g50390

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0005465
Physical Location & Seq
Forward (+)
83738811 .. 83739242
432 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG614600.1

Sequence Viewer

Length: 432 bp
ATGAGCAGGGATTACAAGATTAAGCCCCGTGCTATGCATTATGCTTGTATGATTGAATTGCTAGGACAAGAGGGCAATTTAGATGAAGCGTTTGCAATAATAAGAACTGCTCCCTTAAAGCCTACAGCAAACATGTGGGCTGCTCTGCTGACAGCTTGTCGAATCCATGAGAATTTAGAGCTTGGCAAAATTGCTGCTGAAGAGCTCTATGGGATGGAGCCTGAGAAGCAAAGTAATTATGTTGTGCTCCTAAATATATACAACAGTTGTGGAAAGTTGAAGGAAGTTGCTGCTGTTGCTCAGACTCTAAGAAGAAAGGATTTGAGAATGCTTCCGGCATGCAGTTGGATTGAAATTAAGAAACAGGTACATATTTTTCATTCCGGAGATGAAAGGCATGCCCAAACAAGAGAGATTTACGAGAAATTGTAG

Protein Analysis

143

Amino Acids

16.41

Weight (kDa)

8.33

Isoelectric Point (pI)

66.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E_motif PF20431 56 - 118 5.7e-14 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 383
AcsI RAATTY 1 cut(s) 172
AcuI CTGAAG 1 cut(s) 219
AfaI GTAC 1 cut(s) 369
AflIII ACRYGT 1 cut(s) 132
AgsI TTSAA 3 cut(s) 56, 280, 353
AhdI GACNNNNNGTC 1 cut(s) 156
AluBI AGCT 3 cut(s) 155, 181, 205
AluI AGCT 3 cut(s) 155, 181, 205
Alw21I GWGCWC 2 cut(s) 207, 249
Aor13HI TCCGGA 1 cut(s) 383
ApeKI GCWGC 3 cut(s) 140, 194, 290
ApoI RAATTY 1 cut(s) 172
BanII GRGCYC 1 cut(s) 207
Bbv12I GWGCWC 2 cut(s) 207, 249
BbvI GCAGC 3 cut(s) 127, 181, 277
BccI CCATC 1 cut(s) 208
BfaI CTAG 1 cut(s) 62
BfmI CTRYAG 1 cut(s) 123
BisI GCNGC 3 cut(s) 141, 195, 291
BlsI GCNGC 3 cut(s) 142, 196, 292
BmeRI GACNNNNNGTC 1 cut(s) 156
BmiI GGNNCC 1 cut(s) 219
BsaWI WCCGGW 1 cut(s) 383
BseAI TCCGGA 1 cut(s) 383
BseGI GGATG 1 cut(s) 219
BseMII CTCAG 2 cut(s) 213, 314
BseXI GCAGC 3 cut(s) 127, 181, 277
BsiHKAI GWGCWC 2 cut(s) 207, 249
BsiSI CCGG 2 cut(s) 335, 384
BsmI GAATGC 1 cut(s) 333
Bsp1286I GDGCHC 2 cut(s) 207, 249
Bsp13I TCCGGA 1 cut(s) 383
BspCNI CTCAG 2 cut(s) 214, 313
BspEI TCCGGA 1 cut(s) 383
BspLI GGNNCC 1 cut(s) 219
BspQI GCTCTTC 1 cut(s) 195
Bst4CI ACNGT 1 cut(s) 266
Bst6I CTCTTC 1 cut(s) 195
BstC8I GCNNGC 2 cut(s) 340, 399
BstDEI CTNAG 3 cut(s) 222, 300, 308
BstF5I GGATG 1 cut(s) 219
BstMWI GCNNNNNNNGC 2 cut(s) 226, 296
BstNSI RCATGY 3 cut(s) 136, 342, 401
BstSFI CTRYAG 1 cut(s) 123
BstV1I GCAGC 3 cut(s) 127, 181, 277
BtsCI GGATG 1 cut(s) 219
Cac8I GCNNGC 2 cut(s) 340, 399
Csp6I GTAC 1 cut(s) 368
CspCI CAANNNNNGTGG 2 cut(s) 250, 285
CviAII CATG 4 cut(s) 133, 167, 339, 398
CviJI RGCY 7 cut(s) 25, 121, 140, 155, 181, 205, 220
CviKI_1 RGCY 7 cut(s) 25, 121, 140, 155, 181, 205, 220
CviQI GTAC 1 cut(s) 368
DdeI CTNAG 3 cut(s) 222, 300, 308
DriI GACNNNNNGTC 1 cut(s) 156
Eam1104I CTCTTC 1 cut(s) 195
Eam1105I GACNNNNNGTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 195
Ecl136II GAGCTC 1 cut(s) 205
Eco24I GRGCYC 1 cut(s) 207
Eco53kI GAGCTC 1 cut(s) 205
Eco57I CTGAAG 1 cut(s) 219
EcoICRI GAGCTC 1 cut(s) 205
EcoT22I ATGCAT 1 cut(s) 39
EcoT38I GRGCYC 1 cut(s) 207
FaeI CATG 4 cut(s) 136, 170, 342, 401
FatI CATG 4 cut(s) 132, 166, 338, 397
Fnu4HI GCNGC 3 cut(s) 141, 195, 291
FokI GGATG 1 cut(s) 226
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 3 cut(s) 141, 195, 291
FspBI CTAG 1 cut(s) 62
GluI GCNGC 3 cut(s) 141, 195, 291
HapII CCGG 2 cut(s) 335, 384
Hin1II CATG 4 cut(s) 136, 170, 342, 401
HinfI GANTC 2 cut(s) 162, 304
HpaII CCGG 2 cut(s) 335, 384
Hpy188I TCNGA 1 cut(s) 303
Hpy188III TCNNGA 1 cut(s) 384
HpyAV CCTTC 1 cut(s) 274
HpyCH4III ACNGT 1 cut(s) 266
HpyCH4V TGCA 3 cut(s) 37, 95, 342
HpyF10VI GCNNNNNNNGC 2 cut(s) 226, 296
HpyF3I CTNAG 3 cut(s) 222, 300, 308
Hsp92II CATG 4 cut(s) 136, 170, 342, 401
Kpn2I TCCGGA 1 cut(s) 383
LguI GCTCTTC 1 cut(s) 195
LmnI GCTCC 3 cut(s) 115, 217, 252
LpnPI CCDG 4 cut(s) 234, 348, 350, 397
Lsp1109I GCAGC 3 cut(s) 127, 181, 277
MaeI CTAG 1 cut(s) 62
MboII GAAGA 2 cut(s) 212, 324
MhlI GDGCHC 2 cut(s) 207, 249
MluCI AATT 7 cut(s) 56, 76, 172, 189, 235, 354, 425
MlyI GAGTC 1 cut(s) 298
MmeI TCCRAC 1 cut(s) 326
MnlI CCTC 1 cut(s) 64
Mph1103I ATGCAT 1 cut(s) 39
MroI TCCGGA 1 cut(s) 383
MseI TTAA 3 cut(s) 21, 116, 357
MspI CCGG 2 cut(s) 335, 384
Mva1269I GAATGC 1 cut(s) 333
MwoI GCNNNNNNNGC 2 cut(s) 226, 296
NlaIII CATG 4 cut(s) 136, 170, 342, 401
NlaIV GGNNCC 1 cut(s) 219
NsiI ATGCAT 1 cut(s) 39
NspI RCATGY 3 cut(s) 136, 342, 401
PaeI GCATGC 2 cut(s) 342, 401
PciI ACATGT 1 cut(s) 132
PciSI GCTCTTC 1 cut(s) 195
PctI GAATGC 1 cut(s) 333
PfeI GAWTC 1 cut(s) 162
PkrI GCNGC 3 cut(s) 142, 196, 292
PleI GAGTC 1 cut(s) 298
PpsI GAGTC 1 cut(s) 298
PscI ACATGT 1 cut(s) 132
Psp124BI GAGCTC 1 cut(s) 207
PspN4I GGNNCC 1 cut(s) 219
RsaI GTAC 1 cut(s) 369
RsaNI GTAC 1 cut(s) 368
SacI GAGCTC 1 cut(s) 207
SapI GCTCTTC 1 cut(s) 195
SaqAI TTAA 3 cut(s) 21, 116, 357
SatI GCNGC 3 cut(s) 141, 195, 291
SchI GAGTC 1 cut(s) 298
SduI GDGCHC 2 cut(s) 207, 249
SetI ASST 4 cut(s) 157, 183, 207, 369
SfcI CTRYAG 1 cut(s) 123
SphI GCATGC 2 cut(s) 342, 401
Sse9I AATT 7 cut(s) 56, 76, 172, 189, 235, 354, 425
SspMI CTAG 1 cut(s) 62
SstI GAGCTC 1 cut(s) 207
TaaI ACNGT 1 cut(s) 266
TaqI TCGA 1 cut(s) 160
TasI AATT 7 cut(s) 56, 76, 172, 189, 235, 354, 425
TfiI GAWTC 1 cut(s) 162
Tru1I TTAA 3 cut(s) 21, 116, 357
Tru9I TTAA 3 cut(s) 21, 116, 357
TseI GCWGC 3 cut(s) 140, 194, 290
TspDTI ATGAA 3 cut(s) 99, 368, 405
XapI RAATTY 1 cut(s) 172
XceI RCATGY 3 cut(s) 136, 342, 401
XspI CTAG 1 cut(s) 62
Zsp2I ATGCAT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.