Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0004220

Physical Location & Seq

Genomic Coordinates Reverse (-)

45861727 .. 45868409

Gene Structure 6683 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1190700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1710 bp

ATGGGGGACTATAACAACATGGGTTTCCTGGTCTTAGCTCTTGTAGGGTTTATGTTCCTGAGCAAGGCTAATGGCGCTATCCATTATTATGATTTCGTTCTGCAAGAAACAAATTTTACAAGGCTTTGTAGCACAAAGAGCATTTTAACTGTGAATGGAACTCTTCCGGGACCAACCATCACTGTTAACAGAGGAGACACCGCTTTTGTCAATGTTCACAACCAAGGATATTACGGTCTCACTGTTCACTGGCATGGAGTGAAGAACCCAAGAAATCCATGGTCCGATGGACCTGAAAACATCACTCAGTGCCCTATTCAACCAGGAACAAACTTTACGTACGAAGTTATATTCTCCAATGAAGAAGGAACATTATGGTGGCATGCTCATAGTGACTGGACACGCGCCACCGTCCATGGTGCAATTGTTATTCTACCGGCTGCCGGCACCACTTATCCATTTCCAACACCAGATGCACAAGAAACAGTTATTCTAGGATCATGGTTTAAGGGAGATGTGATGGAAATAATAGAAGAGGCTCTCGCAAAAGGTGGCGATCCAAATGTTTCAGATGCCTTCACAATTAACGGACAACCCGGAGATTTGTATAGTTGTTCCAATGAGACAACATACCGATGGATGGTTGATTACGGTAAGACCTATCTTCTCCGGGTAATCAACGCCGTGATGAACGAAGAACAGTTCTTCGCCATCGCGAACCACACCCTCACAGTCGTAGCACAAGATGCTTCATACATAAAACCTATAACCACCACTTACATCATGATAACTCCAGGCCAAACCATGGACATTTTAGTCACCGCAAACCAACCTCCCAGCCAGTATTACATGGCTTCTCATTCTTTTATGGATGGAACTAATGTTGCACATCACAACGGCACCACCAGTGCAATTGTTCAATATAACGGCAACTACAGCACCCCATCAATCACTCCCCTTCCAACTCTTCCGGCTGAAGCCAATATAACTGCTGCTGATAACTTCACTAAGCAAGTCAGGGCCTTGGCAAGCGAAGAGCACCCTATTGATGTCCCACTTGAAATTCATTCCAGGATCTTTATAGCTATTGCAATCAATGAAAGAATTTGTGCTAATTCTTCATGTGAAGGCCCGGATAATAATGCACTCTCTGCAAGCTTAAACAATATCAGTTTCGTGATTCCATCTGTTGATGTACTGCAAGCATACTATCGGGGAAATAACAATGGAGTTTACAGTTCCAATTTCCCGGACAATCCGCCTAATCCTTACAACTACACTGGAAACGTGACTAGCAGCACAATATATTCGGACTTTGGGACGAGGGTGAGGATGATTGAATATGGTGAAGGAGTTGAGATCATCTTCCAAGGAACCAACATCATCGCAGCTGAGAACCATCCAATGCATCTCCATGGTTTCAGCTTCTATTTGGTCGGAACTGGTTACGGAAATTTTGACAATACAACATCACCCAAGACCTACAATTTGGTTGATCCCCCAGAAGTAAACACCATTGGAGTTCCAAAGAATGGATGGGCAACCATCAGGTTTAAAGCCGATAATCCTGGAGTATGGTTTATGCATTGTCATTTGGAAAGACATACCAGCTGGGGAATGGATACTGTGCTCATAGTTAGGAATGGAAATACCACAGAAACCAGCATCCGCCCTCCGCCTGCTTATATGCCTCCTTGTTCCAATTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

570

Amino Acids

62.77

Weight (kDa)

5.18

Isoelectric Point (pI)

31.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cu-oxidase_3	PF07732	35 - 147	4.3e-38	Multicopper oxidase
Cu-oxidase	PF00394	162 - 311	1.8e-41	Multicopper oxidase
Cu-oxidase_2	PF07731	416 - 549	2.3e-39	Multicopper oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004220)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g12610 FvH4_2g12610 FvH4_2g21650
malus_domestica	MD00G1014800.v1.1 MD00G1190700.v1.1 MD02G1264400.v1.1 MD10G1042600.v1.1
prunus_persica	Prupe.8G047100_v2.0.a1 Prupe.8G047200_v2.0.a1 Prupe.8G047300_v2.0.a1 Prupe.8G047500_v2.0.a1 Prupe.8G047500_v2.0.a1 Prupe.8G047900_v2.0.a1 Prupe.8G048100_v2.0.a1
pyrus_communis	pycom05g02900 pycom10g02970
rosa_chinensis	RchiOBHm_Chr6g0273201 RchiOBHm_Chr6g0288321
rosa_laevigata	RLG00000012414 RLG00000013632 RLG00000013639
rosa_multiflora	Rmu_sc0000239.1_g000090
rosa_roxburghii	Rroxscaffold_7G00180140 Rroxscaffold_7G00195550
rosa_rugosa	Rorug06G0073100 Rorug06G0195600
rosa_samantha	Rh6BG192200 Rh6BG312600 Rh6CG191600 Rh6CG320400 Rh6DG183000 Rh6DG305600
rosa_wichuraiana	Rw6G016390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	815
AccB1I	GGYRCC	2 cut(s)	446, 899
AccB7I	CCANNNNNTGG	2 cut(s)	805, 1532
AccII	CGCG	2 cut(s)	405, 716
AciI	CCGC	5 cut(s)	201, 822, 1259, 1669, 1676
AclWI	GGATC	4 cut(s)	505, 551, 1082, 1490
AcsI	RAATTY	4 cut(s)	112, 1062, 1104, 1453
AcuI	CTGAAG	1 cut(s)	996
AdeI	CACNNNGTG	1 cut(s)	309
AfaI	GTAC	2 cut(s)	341, 1197
AfiI	CCNNNNNNNGG	5 cut(s)	64, 443, 640, 805, 1532
AgsI	TTSAA	4 cut(s)	320, 920, 1061, 1340
AjnI	CCWGG	5 cut(s)	27, 322, 793, 1070, 1567
AluBI	AGCT	6 cut(s)	38, 1085, 1158, 1391, 1425, 1611
AluI	AGCT	6 cut(s)	38, 1085, 1158, 1391, 1425, 1611
Alw21I	GWGCWC	2 cut(s)	1041, 1632
Alw26I	GTCTC	3 cut(s)	189, 242, 617
AlwI	GGATC	4 cut(s)	505, 551, 1082, 1490
AoxI	GGCC	3 cut(s)	796, 1020, 1129
ApeKI	GCWGC	4 cut(s)	440, 992, 1296, 1388
ApoI	RAATTY	4 cut(s)	112, 1062, 1104, 1453
ArsI	GACNNNNNNTTYG	4 cut(s)	188, 220, 1471, 1503
AspLEI	GCGC	2 cut(s)	77, 407
AspS9I	GGNCC	5 cut(s)	170, 282, 290, 1020, 1130
AsuC2I	CCSGG	5 cut(s)	168, 597, 671, 1133, 1250
AsuHPI	GGTGA	4 cut(s)	811, 1339, 1358, 1464
AvaII	GGWCC	3 cut(s)	170, 282, 290
BaeGI	GKGCMC	1 cut(s)	314
BanI	GGYRCC	2 cut(s)	446, 899
Bbv12I	GWGCWC	2 cut(s)	1041, 1632
BbvI	GCAGC	4 cut(s)	427, 979, 1308, 1400
BceAI	ACGGC	3 cut(s)	668, 913, 943
BciT130I	CCWGG	5 cut(s)	29, 324, 795, 1072, 1569
BciVI	GTATCC	1 cut(s)	1615
BcnI	CCSGG	5 cut(s)	168, 597, 671, 1133, 1250
BcoDI	GTCTC	3 cut(s)	189, 242, 617
BfaI	CTAG	2 cut(s)	494, 1293
BfmI	CTRYAG	1 cut(s)	934
BfoI	RGCGCY	1 cut(s)	78
BfuI	GTATCC	1 cut(s)	1615
BisI	GCNGC	4 cut(s)	441, 993, 1297, 1389
BlsI	GCNGC	4 cut(s)	442, 994, 1298, 1390
Bme18I	GGWCC	3 cut(s)	170, 282, 290
BmgT120I	GGNCC	5 cut(s)	170, 282, 290, 1020, 1130
BmiI	GGNNCC	4 cut(s)	171, 448, 901, 1375
BmsI	GCATC	5 cut(s)	463, 562, 736, 1417, 1674
BpmI	CTGGAG	2 cut(s)	777, 1590
Bpu10I	CCTNAGC	1 cut(s)	59
BpuMI	CCSGG	5 cut(s)	168, 597, 671, 1133, 1250
BsaAI	YACGTR	1 cut(s)	339
BsaI	GGTCTC	1 cut(s)	242
BsaJI	CCNNGG	7 cut(s)	223, 278, 415, 804, 1023, 1369, 1414
Bsc4I	CCNNNNNNNGG	5 cut(s)	64, 443, 640, 805, 1532
Bse118I	RCCGGY	2 cut(s)	436, 443
Bse1I	ACTGG	6 cut(s)	254, 401, 841, 906, 1285, 1447
BseBI	CCWGG	5 cut(s)	29, 324, 795, 1072, 1569
BseDI	CCNNGG	7 cut(s)	223, 278, 415, 804, 1023, 1369, 1414
BseGI	GGATG	6 cut(s)	645, 877, 1338, 1399, 1541, 1665
BseLI	CCNNNNNNNGG	5 cut(s)	64, 443, 640, 805, 1532
BseMII	CTCAG	3 cut(s)	50, 320, 1383
BseNI	ACTGG	6 cut(s)	254, 401, 841, 906, 1285, 1447
BseRI	GAGGAG	1 cut(s)	207
BseSI	GKGCMC	1 cut(s)	314
BseXI	GCAGC	4 cut(s)	427, 979, 1308, 1400
BseYI	CCCAGC	2 cut(s)	836, 1611
Bsh1236I	CGCG	2 cut(s)	405, 716
BshFI	GGCC	3 cut(s)	798, 1022, 1131
BshNI	GGYRCC	2 cut(s)	446, 899
BsiHKAI	GWGCWC	2 cut(s)	1041, 1632
BsiSI	CCGG	8 cut(s)	167, 437, 444, 597, 670, 971, 1133, 1250
BsiWI	CGTACG	1 cut(s)	339
BslFI	GGGAC	4 cut(s)	20, 183, 1037, 1333
BslI	CCNNNNNNNGG	5 cut(s)	64, 443, 640, 805, 1532
BsmAI	GTCTC	3 cut(s)	189, 242, 617
BsmFI	GGGAC	4 cut(s)	20, 183, 1037, 1333
BsnI	GGCC	3 cut(s)	798, 1022, 1131
Bso31I	GGTCTC	1 cut(s)	242
Bsp1286I	GDGCHC	3 cut(s)	314, 1041, 1632
Bsp143I	GATC	5 cut(s)	497, 556, 1074, 1359, 1495
Bsp19I	CCATGG	4 cut(s)	278, 415, 804, 1414
Bsp68I	TCGCGA	1 cut(s)	716
BspACI	CCGC	5 cut(s)	201, 822, 1259, 1669, 1676
BspANI	GGCC	3 cut(s)	798, 1022, 1131
BspCNI	CTCAG	3 cut(s)	51, 319, 1384
BspFNI	CGCG	2 cut(s)	405, 716
BspHI	TCATGA	1 cut(s)	783
BspLI	GGNNCC	4 cut(s)	171, 448, 901, 1375
BspPI	GGATC	4 cut(s)	505, 551, 1082, 1490
BspQI	GCTCTTC	1 cut(s)	1029
BspT107I	GGYRCC	2 cut(s)	446, 899
BspTNI	GGTCTC	1 cut(s)	242
BsrFI	RCCGGY	2 cut(s)	436, 443
BsrI	ACTGG	6 cut(s)	254, 401, 841, 906, 1285, 1447
BssAI	RCCGGY	2 cut(s)	436, 443
BssECI	CCNNGG	7 cut(s)	223, 278, 415, 804, 1023, 1369, 1414
BssMI	GATC	5 cut(s)	497, 556, 1074, 1359, 1495
BssT1I	CCWWGG	7 cut(s)	223, 278, 415, 804, 1023, 1369, 1414
Bst2UI	CCWGG	5 cut(s)	29, 324, 795, 1072, 1569
Bst6I	CTCTTC	4 cut(s)	168, 528, 972, 1029
BstAPI	GCANNNNNTGC	2 cut(s)	746, 1151
BstBAI	YACGTR	1 cut(s)	339
BstC8I	GCNNGC	6 cut(s)	384, 445, 1030, 1156, 1203, 1680
BstDEI	CTNAG	6 cut(s)	34, 59, 306, 1008, 1392, 1707
BstDSI	CCRYGG	4 cut(s)	278, 415, 804, 1414
BstENI	CCTNNNNNAGG	1 cut(s)	62
BstF5I	GGATG	6 cut(s)	645, 877, 1338, 1399, 1541, 1665
BstFNI	CGCG	2 cut(s)	405, 716
BstH2I	RGCGCY	1 cut(s)	78
BstHHI	GCGC	2 cut(s)	77, 407
BstKTI	GATC	5 cut(s)	500, 559, 1077, 1362, 1498
BstMAI	GTCTC	3 cut(s)	189, 242, 617
BstMBI	GATC	5 cut(s)	497, 556, 1074, 1359, 1495
BstMWI	GCNNNNNNNGC	5 cut(s)	74, 138, 746, 936, 1151
BstNI	CCWGG	5 cut(s)	29, 324, 795, 1072, 1569
BstNSI	RCATGY	1 cut(s)	386
BstSFI	CTRYAG	1 cut(s)	934
BstSLI	GKGCMC	1 cut(s)	314
BstSNI	TACGTA	1 cut(s)	339
BstUI	CGCG	2 cut(s)	405, 716
BstV1I	GCAGC	4 cut(s)	427, 979, 1308, 1400
BstX2I	RGATCY	1 cut(s)	1074
BstYI	RGATCY	1 cut(s)	1074
BsuI	GTATCC	1 cut(s)	1615
BsuRI	GGCC	3 cut(s)	798, 1022, 1131
BtgI	CCRYGG	4 cut(s)	278, 415, 804, 1414
BtgZI	GCGATG	2 cut(s)	697, 1369
BtsCI	GGATG	6 cut(s)	645, 877, 1338, 1399, 1541, 1665
BtsIMutI	CAGTG	6 cut(s)	180, 240, 247, 314, 913, 1278
BtuMI	TCGCGA	1 cut(s)	716
Cac8I	GCNNGC	6 cut(s)	384, 445, 1030, 1156, 1203, 1680
CciI	TCATGA	1 cut(s)	783
CfoI	GCGC	2 cut(s)	77, 407
Cfr10I	RCCGGY	2 cut(s)	436, 443
Cfr13I	GGNCC	5 cut(s)	170, 282, 290, 1020, 1130
Csp6I	GTAC	2 cut(s)	340, 1196
CviQI	GTAC	2 cut(s)	340, 1196
DdeI	CTNAG	6 cut(s)	34, 59, 306, 1008, 1392, 1707
DpnI	GATC	5 cut(s)	499, 558, 1076, 1361, 1497
DpnII	GATC	5 cut(s)	497, 556, 1074, 1359, 1495
DraI	TTTAAA	1 cut(s)	1555
DraIII	CACNNNGTG	1 cut(s)	309
DrdI	GACNNNNNNGTC	1 cut(s)	815
DseDI	GACNNNNNNGTC	1 cut(s)	815
Eam1104I	CTCTTC	4 cut(s)	168, 528, 972, 1029
EarI	CTCTTC	4 cut(s)	168, 528, 972, 1029
EciI	GGCGGA	3 cut(s)	1248, 1658, 1665
Eco105I	TACGTA	1 cut(s)	339
Eco130I	CCWWGG	7 cut(s)	223, 278, 415, 804, 1023, 1369, 1414
Eco31I	GGTCTC	1 cut(s)	242
Eco47I	GGWCC	3 cut(s)	170, 282, 290
Eco57I	CTGAAG	1 cut(s)	996
EcoNI	CCTNNNNNAGG	1 cut(s)	62
EcoO109I	RGGNCCY	1 cut(s)	1020
EcoRII	CCWGG	5 cut(s)	27, 322, 793, 1070, 1567
EcoT14I	CCWWGG	7 cut(s)	223, 278, 415, 804, 1023, 1369, 1414
EcoT22I	ATGCAT	2 cut(s)	1410, 1587
ErhI	CCWWGG	7 cut(s)	223, 278, 415, 804, 1023, 1369, 1414
FaqI	GGGAC	4 cut(s)	20, 183, 1037, 1333
Fnu4HI	GCNGC	4 cut(s)	441, 993, 1297, 1389
FokI	GGATG	6 cut(s)	652, 884, 1345, 1386, 1548, 1652
Fsp4HI	GCNGC	4 cut(s)	441, 993, 1297, 1389
FspBI	CTAG	2 cut(s)	494, 1293
GlaI	GCGC	2 cut(s)	76, 406
GluI	GCNGC	4 cut(s)	441, 993, 1297, 1389
GsaI	CCCAGC	2 cut(s)	840, 1615
GsuI	CTGGAG	2 cut(s)	777, 1590
HaeII	RGCGCY	1 cut(s)	78
HaeIII	GGCC	3 cut(s)	798, 1022, 1131
HapII	CCGG	8 cut(s)	167, 437, 444, 597, 670, 971, 1133, 1250
HhaI	GCGC	2 cut(s)	77, 407
Hin6I	GCGC	2 cut(s)	75, 405
HinP1I	GCGC	2 cut(s)	75, 405
HincII	GTYRAC	1 cut(s)	187
HindII	GTYRAC	1 cut(s)	187
HindIII	AAGCTT	1 cut(s)	1156
HinfI	GANTC	1 cut(s)	1180
HpaI	GTTAAC	1 cut(s)	187
HpaII	CCGG	8 cut(s)	167, 437, 444, 597, 670, 971, 1133, 1250
HphI	GGTGA	4 cut(s)	811, 1339, 1358, 1464
Hpy166II	GTNNAC	5 cut(s)	187, 217, 247, 1234, 1510
Hpy188I	TCNGA	4 cut(s)	286, 571, 1312, 1439
Hpy188III	TCNNGA	4 cut(s)	58, 715, 784, 1177
Hpy8I	GTNNAC	5 cut(s)	187, 217, 247, 1234, 1510
HpyAV	CCTTC	5 cut(s)	359, 586, 968, 1121, 1343
HpyCH4IV	ACGT	2 cut(s)	338, 1287
HpyF10VI	GCNNNNNNNGC	5 cut(s)	74, 138, 746, 936, 1151
HpyF3I	CTNAG	6 cut(s)	34, 59, 306, 1008, 1392, 1707
HpySE526I	ACGT	2 cut(s)	338, 1287
HspAI	GCGC	2 cut(s)	75, 405
KroI	GCCGGC	1 cut(s)	443
KroNI	GCCGGC	1 cut(s)	445
KspAI	GTTAAC	1 cut(s)	187
Kzo9I	GATC	5 cut(s)	497, 556, 1074, 1359, 1495
LguI	GCTCTTC	1 cut(s)	1029
Lsp1109I	GCAGC	4 cut(s)	427, 979, 1308, 1400
LweI	GCATC	5 cut(s)	463, 562, 736, 1417, 1674
MaeI	CTAG	2 cut(s)	494, 1293
MaeII	ACGT	2 cut(s)	338, 1287
MaeIII	GTNAC	4 cut(s)	392, 817, 1288, 1445
MalI	GATC	5 cut(s)	499, 558, 1076, 1361, 1497
MboI	GATC	5 cut(s)	497, 556, 1074, 1359, 1495
MfeI	CAATTG	2 cut(s)	423, 912
MflI	RGATCY	1 cut(s)	1074
MhlI	GDGCHC	3 cut(s)	314, 1041, 1632
MmeI	TCCRAC	3 cut(s)	488, 986, 1417
MnlI	CCTC	8 cut(s)	185, 529, 737, 843, 1317, 1323, 1683, 1701
Mph1103I	ATGCAT	2 cut(s)	1410, 1587
MroNI	GCCGGC	1 cut(s)	443
MseI	TTAA	6 cut(s)	146, 186, 507, 585, 1160, 1554
MslI	CAYNNNNRTG	5 cut(s)	87, 252, 376, 634, 1413
MspA1I	CMGCKG	2 cut(s)	1391, 1611
MspI	CCGG	8 cut(s)	167, 437, 444, 597, 670, 971, 1133, 1250
MunI	CAATTG	2 cut(s)	423, 912
MvaI	CCWGG	5 cut(s)	29, 324, 795, 1072, 1569
MvnI	CGCG	2 cut(s)	405, 716
MwoI	GCNNNNNNNGC	5 cut(s)	74, 138, 746, 936, 1151
NaeI	GCCGGC	1 cut(s)	445
NciI	CCSGG	5 cut(s)	168, 597, 671, 1133, 1250
NcoI	CCATGG	4 cut(s)	278, 415, 804, 1414
NdeII	GATC	5 cut(s)	497, 556, 1074, 1359, 1495
NgoMIV	GCCGGC	1 cut(s)	443
NlaIV	GGNNCC	4 cut(s)	171, 448, 901, 1375
NmuCI	GTSAC	3 cut(s)	392, 817, 1288
NruI	TCGCGA	1 cut(s)	716
NsiI	ATGCAT	2 cut(s)	1410, 1587
NspI	RCATGY	1 cut(s)	386
PaeI	GCATGC	1 cut(s)	386
PagI	TCATGA	1 cut(s)	783
PciSI	GCTCTTC	1 cut(s)	1029
PdiI	GCCGGC	1 cut(s)	445
PfeI	GAWTC	1 cut(s)	1180
Pfl23II	CGTACG	1 cut(s)	339
PflMI	CCANNNNNTGG	2 cut(s)	805, 1532
PfoI	TCCNGGA	4 cut(s)	166, 1070, 1248, 1567
PkrI	GCNGC	4 cut(s)	442, 994, 1298, 1390
Ppu21I	YACGTR	1 cut(s)	339
Psp6I	CCWGG	5 cut(s)	27, 322, 793, 1070, 1567
PspFI	CCCAGC	2 cut(s)	836, 1611
PspGI	CCWGG	5 cut(s)	27, 322, 793, 1070, 1567
PspLI	CGTACG	1 cut(s)	339
PspN4I	GGNNCC	4 cut(s)	171, 448, 901, 1375
PspPI	GGNCC	5 cut(s)	170, 282, 290, 1020, 1130
PsrI	GAACNNNNNNTAC	2 cut(s)	319, 351
PsuI	RGATCY	1 cut(s)	1074
PvuII	CAGCTG	2 cut(s)	1391, 1611
RruI	TCGCGA	1 cut(s)	716
RsaI	GTAC	2 cut(s)	341, 1197
RsaNI	GTAC	2 cut(s)	340, 1196
RseI	CAYNNNNRTG	5 cut(s)	87, 252, 376, 634, 1413
SapI	GCTCTTC	1 cut(s)	1029
SaqAI	TTAA	6 cut(s)	146, 186, 507, 585, 1160, 1554
SatI	GCNGC	4 cut(s)	441, 993, 1297, 1389
Sau3AI	GATC	5 cut(s)	497, 556, 1074, 1359, 1495
Sau96I	GGNCC	5 cut(s)	170, 282, 290, 1020, 1130
SduI	GDGCHC	3 cut(s)	314, 1041, 1632
SfaNI	GCATC	5 cut(s)	463, 562, 736, 1417, 1674
SfcI	CTRYAG	1 cut(s)	934
SinI	GGWCC	3 cut(s)	170, 282, 290
SmiMI	CAYNNNNRTG	5 cut(s)	87, 252, 376, 634, 1413
SnaBI	TACGTA	1 cut(s)	339
SphI	GCATGC	1 cut(s)	386
SsiI	CCGC	5 cut(s)	201, 822, 1259, 1669, 1676
SspMI	CTAG	2 cut(s)	494, 1293
StyI	CCWWGG	7 cut(s)	223, 278, 415, 804, 1023, 1369, 1414
TaiI	ACGT	2 cut(s)	341, 1290
TatI	WGTACW	1 cut(s)	1195
TfiI	GAWTC	1 cut(s)	1180
Tru1I	TTAA	6 cut(s)	146, 186, 507, 585, 1160, 1554
Tru9I	TTAA	6 cut(s)	146, 186, 507, 585, 1160, 1554
TscAI	CASTG	6 cut(s)	187, 247, 254, 314, 913, 1285
TseFI	GTSAC	3 cut(s)	392, 817, 1288
TseI	GCWGC	4 cut(s)	440, 992, 1296, 1388
Tsp45I	GTSAC	3 cut(s)	392, 817, 1288
TspDTI	ATGAA	6 cut(s)	375, 704, 741, 1055, 1110, 1113
TspGWI	ACGGA	2 cut(s)	603, 1464
TspRI	CASTG	6 cut(s)	187, 247, 254, 314, 913, 1285
Van91I	CCANNNNNTGG	2 cut(s)	805, 1532
VpaK11BI	GGWCC	3 cut(s)	170, 282, 290
XagI	CCTNNNNNAGG	1 cut(s)	62
XapI	RAATTY	4 cut(s)	112, 1062, 1104, 1453
XceI	RCATGY	1 cut(s)	386
XcmI	CCANNNNNNNNNTGG	3 cut(s)	276, 1533, 1615
XspI	CTAG	2 cut(s)	494, 1293
Zsp2I	ATGCAT	2 cut(s)	1410, 1587

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.