Rh6CG320400

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0004220
Physical Location & Seq
Reverse (-)
50557400 .. 50558712
1313 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG320400.1

Sequence Viewer

Length: 786 bp
ATGGAAGATTACCGGGATTCCGTAATCTGGGGGGGCGATGTTGAAGGAGCAACTTCTTTCGCGATCAACGGTTATCCCGGACAGCTTGAAACTCCTTGTGATAATGACATGTACAAATTTGTTGTTCACAGCGGAAAAAATTACCATCTCCGCATAATCAATGCGGTGATGGAGGAGGAAATGTTCTTTGCAATTTCCGGTCACAACTTCACGGTTGTTGGACTTGACGGTGCATATCTGACTCCGATACCCACAAACTACATCACGATAACTCCTGGCCAAACGATGGACATATTGCTCACTACGAATCAGACTCCGCGTAGTTACTACATGGGTGGAAAGGCTTTTGTCGACTCCAAATTTGGTACACCCATGAACTCCACAACAAGAGCAATTCTTCAATACCGCAATTATAAAGGCACTGAAACTCCAGTTGATCTTGGAGACCTTCTTCCAGCTGATTCAAAAAACGCGAGTTATGCAAAGGCCTTCACCGAAAGAGTTCGAACCAGCGGATTGACAGGCAACGATGATTACCCGATTACGGTCCCCACTGATTCTGAAATCAATAAGAACTTCACCATCACAATTTCTGTGAATACGCTTCCTTGTCCATATCCTAGTAACAATTGCACGGCGCCGCAAAATTATAATGCAACTTGGACAGGGATGCACGCTGCATCCTTAAACAACCAACGTTTCGTCACCCAAAATGTCAACATACTGCAGGCATATCGCTGGTACGTACCCACTTACACGCGCGCTATGATTGATTTAGCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

261

Amino Acids

29.07

Weight (kDa)

5.17

Isoelectric Point (pI)

35.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase PF00394 17 - 137 5.8e-28 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 414, 651
AccB1I GGYRCC 1 cut(s) 637
AccB7I CCANNNNNTGG 1 cut(s) 286
AccI GTMKAC 1 cut(s) 351
AccII CGCG 5 cut(s) 62, 319, 473, 760, 762
AciI CCGC 7 cut(s) 132, 151, 164, 317, 406, 513, 641
AclI AACGTT 1 cut(s) 697
AcoI YGGCCR 1 cut(s) 277
AcsI RAATTY 2 cut(s) 116, 359
AcyI GRCGYC 1 cut(s) 638
AfaI GTAC 4 cut(s) 113, 367, 743, 747
AfiI CCNNNNNNNGG 3 cut(s) 27, 286, 544
AflIII ACRYGT 1 cut(s) 108
AgsI TTSAA 4 cut(s) 44, 89, 401, 465
AjnI CCWGG 1 cut(s) 274
AloI GAACNNNNNNTCC 2 cut(s) 167, 199
AluBI AGCT 4 cut(s) 85, 458, 779, 783
AluI AGCT 4 cut(s) 85, 458, 779, 783
Alw26I GTCTC 1 cut(s) 438
AoxI GGCC 2 cut(s) 277, 486
ApeKI GCWGC 1 cut(s) 677
ApoI RAATTY 2 cut(s) 116, 359
ArsI GACNNNNNNTTYG 2 cut(s) 344, 376
Asp700I GAANNNNTTC 1 cut(s) 501
AspLEI GCGC 3 cut(s) 640, 762, 764
AspS9I GGNCC 1 cut(s) 547
AsuC2I CCSGG 2 cut(s) 14, 78
AsuHPI GGTGA 4 cut(s) 178, 484, 571, 697
AsuII TTCGAA 1 cut(s) 505
AsuNHI GCTAGC 1 cut(s) 779
AvaII GGWCC 1 cut(s) 547
BalI TGGCCA 1 cut(s) 279
BanI GGYRCC 1 cut(s) 637
BbvI GCAGC 1 cut(s) 664
BccI CCATC 4 cut(s) 153, 163, 280, 590
BceAI ACGGC 1 cut(s) 651
BcgI CGANNNNNNTGC 2 cut(s) 716, 750
BciT130I CCWGG 1 cut(s) 276
BcnI CCSGG 2 cut(s) 14, 78
BcoDI GTCTC 1 cut(s) 438
BfaI CTAG 3 cut(s) 621, 780, 784
BfmI CTRYAG 1 cut(s) 725
BfoI RGCGCY 1 cut(s) 641
BisI GCNGC 2 cut(s) 641, 678
BlsI GCNGC 2 cut(s) 642, 679
Bme1390I CCNGG 3 cut(s) 14, 78, 276
Bme18I GGWCC 1 cut(s) 547
BmgT120I GGNCC 1 cut(s) 547
BmiI GGNNCC 2 cut(s) 549, 639
BmrFI CCNGG 3 cut(s) 14, 78, 276
BmsI GCATC 2 cut(s) 660, 689
BmtI GCTAGC 1 cut(s) 783
BpmI CTGGAG 1 cut(s) 414
Bpu14I TTCGAA 1 cut(s) 505
BpuMI CCSGG 2 cut(s) 14, 78
BsaAI YACGTR 1 cut(s) 745
BsaHI GRCGYC 1 cut(s) 638
BsaI GGTCTC 1 cut(s) 438
BsaWI WCCGGW 1 cut(s) 197
BsaXI ACNNNNNCTCC 6 cut(s) 167, 197, 256, 286, 412, 442
Bsc4I CCNNNNNNNGG 3 cut(s) 27, 286, 544
Bse1I ACTGG 1 cut(s) 431
BseBI CCWGG 1 cut(s) 276
BseGI GGATG 2 cut(s) 675, 680
BseLI CCNNNNNNNGG 3 cut(s) 27, 286, 544
BseNI ACTGG 1 cut(s) 431
BsePI GCGCGC 1 cut(s) 760
BseRI GAGGAG 1 cut(s) 188
BseXI GCAGC 1 cut(s) 664
Bsh1236I CGCG 5 cut(s) 62, 319, 473, 760, 762
BshFI GGCC 2 cut(s) 279, 488
BshNI GGYRCC 1 cut(s) 637
BsiSI CCGG 3 cut(s) 13, 78, 198
BslFI GGGAC 1 cut(s) 533
BslI CCNNNNNNNGG 3 cut(s) 27, 286, 544
BsmAI GTCTC 1 cut(s) 438
BsmFI GGGAC 1 cut(s) 533
BsnI GGCC 2 cut(s) 279, 488
Bso31I GGTCTC 1 cut(s) 438
Bsp119I TTCGAA 1 cut(s) 505
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 2 cut(s) 63, 436
Bsp68I TCGCGA 1 cut(s) 62
BspACI CCGC 7 cut(s) 132, 151, 164, 317, 406, 513, 641
BspANI GGCC 2 cut(s) 279, 488
BspFNI CGCG 5 cut(s) 62, 319, 473, 760, 762
BspLI GGNNCC 2 cut(s) 549, 639
BspMAI CTGCAG 1 cut(s) 729
BspOI GCTAGC 1 cut(s) 783
BspT104I TTCGAA 1 cut(s) 505
BspT107I GGYRCC 1 cut(s) 637
BspTNI GGTCTC 1 cut(s) 438
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 1 cut(s) 431
BssHII GCGCGC 1 cut(s) 760
BssMI GATC 2 cut(s) 63, 436
BssNI GRCGYC 1 cut(s) 638
Bst2UI CCWGG 1 cut(s) 276
Bst4CI ACNGT 4 cut(s) 71, 214, 230, 547
BstACI GRCGYC 1 cut(s) 638
BstAUI TGTACA 1 cut(s) 111
BstBAI YACGTR 1 cut(s) 745
BstBI TTCGAA 1 cut(s) 505
BstC8I GCNNGC 4 cut(s) 675, 729, 762, 781
BstF5I GGATG 2 cut(s) 675, 680
BstFNI CGCG 5 cut(s) 62, 319, 473, 760, 762
BstH2I RGCGCY 1 cut(s) 641
BstHHI GCGC 3 cut(s) 640, 762, 764
BstKTI GATC 2 cut(s) 66, 439
BstMAI GTCTC 1 cut(s) 438
BstMBI GATC 2 cut(s) 63, 436
BstMWI GCNNNNNNNGC 1 cut(s) 479
BstNI CCWGG 1 cut(s) 276
BstNSI RCATGY 1 cut(s) 112
BstSCI CCNGG 3 cut(s) 12, 76, 274
BstSFI CTRYAG 1 cut(s) 725
BstSNI TACGTA 1 cut(s) 745
BstUI CGCG 5 cut(s) 62, 319, 473, 760, 762
BstV1I GCAGC 1 cut(s) 664
BsuRI GGCC 2 cut(s) 279, 488
BtgZI GCGATG 1 cut(s) 51
BtsCI GGATG 2 cut(s) 675, 680
BtsIMutI CAGTG 2 cut(s) 420, 552
BtuMI TCGCGA 1 cut(s) 62
Cac8I GCNNGC 4 cut(s) 675, 729, 762, 781
CfoI GCGC 3 cut(s) 640, 762, 764
Cfr13I GGNCC 1 cut(s) 547
Csp6I GTAC 4 cut(s) 112, 366, 742, 746
CviAII CATG 3 cut(s) 109, 331, 373
CviJI RGCY 7 cut(s) 85, 279, 344, 458, 488, 779, 783
CviKI_1 RGCY 7 cut(s) 85, 279, 344, 458, 488, 779, 783
CviQI GTAC 4 cut(s) 112, 366, 742, 746
DinI GGCGCC 1 cut(s) 639
DpnI GATC 2 cut(s) 65, 438
DpnII GATC 2 cut(s) 63, 436
EaeI YGGCCR 1 cut(s) 277
Eco105I TACGTA 1 cut(s) 745
Eco147I AGGCCT 1 cut(s) 488
Eco31I GGTCTC 1 cut(s) 438
Eco47I GGWCC 1 cut(s) 547
EcoRII CCWGG 1 cut(s) 274
EgeI GGCGCC 1 cut(s) 639
EheI GGCGCC 1 cut(s) 639
FaeI CATG 3 cut(s) 112, 334, 376
FaqI GGGAC 1 cut(s) 533
FatI CATG 3 cut(s) 108, 330, 372
FblI GTMKAC 1 cut(s) 351
Fnu4HI GCNGC 2 cut(s) 641, 678
FokI GGATG 2 cut(s) 667, 682
Fsp4HI GCNGC 2 cut(s) 641, 678
FspBI CTAG 3 cut(s) 621, 780, 784
GlaI GCGC 3 cut(s) 639, 761, 763
GluI GCNGC 2 cut(s) 641, 678
GsuI CTGGAG 1 cut(s) 414
HaeII RGCGCY 1 cut(s) 641
HaeIII GGCC 2 cut(s) 279, 488
HapII CCGG 3 cut(s) 13, 78, 198
HhaI GCGC 3 cut(s) 640, 762, 764
Hin1I GRCGYC 1 cut(s) 638
Hin1II CATG 3 cut(s) 112, 334, 376
Hin6I GCGC 3 cut(s) 638, 760, 762
HinP1I GCGC 3 cut(s) 638, 760, 762
HincII GTYRAC 2 cut(s) 352, 718
HindII GTYRAC 2 cut(s) 352, 718
HinfI GANTC 7 cut(s) 17, 241, 307, 313, 353, 461, 557
HpaII CCGG 3 cut(s) 13, 78, 198
HphI GGTGA 4 cut(s) 178, 484, 571, 697
Hpy166II GTNNAC 4 cut(s) 127, 352, 368, 718
Hpy188I TCNGA 4 cut(s) 240, 246, 312, 562
Hpy188III TCNNGA 2 cut(s) 61, 265
Hpy8I GTNNAC 4 cut(s) 127, 352, 368, 718
HpyAV CCTTC 3 cut(s) 38, 458, 499
HpyCH4III ACNGT 4 cut(s) 71, 214, 230, 547
HpyCH4IV ACGT 2 cut(s) 697, 744
HpyCH4V TGCA 8 cut(s) 191, 233, 482, 633, 656, 673, 680, 727
HpyF10VI GCNNNNNNNGC 1 cut(s) 479
HpySE526I ACGT 2 cut(s) 697, 744
Hsp92I GRCGYC 1 cut(s) 638
Hsp92II CATG 3 cut(s) 112, 334, 376
HspAI GCGC 3 cut(s) 638, 760, 762
KasI GGCGCC 1 cut(s) 637
Kzo9I GATC 2 cut(s) 63, 436
LmnI GCTCC 1 cut(s) 47
Lsp1109I GCAGC 1 cut(s) 664
LweI GCATC 2 cut(s) 660, 689
MaeI CTAG 3 cut(s) 621, 780, 784
MaeII ACGT 2 cut(s) 697, 744
MaeIII GTNAC 4 cut(s) 200, 323, 623, 703
MalI GATC 2 cut(s) 65, 438
MboI GATC 2 cut(s) 63, 436
MboII GAAGA 3 cut(s) 17, 389, 443
MfeI CAATTG 1 cut(s) 628
MlsI TGGCCA 1 cut(s) 279
MluCI AATT 9 cut(s) 116, 139, 192, 359, 393, 409, 588, 628, 646
MluNI TGGCCA 1 cut(s) 279
Mly113I GGCGCC 1 cut(s) 638
MlyI GAGTC 3 cut(s) 235, 307, 347
MmeI TCCRAC 1 cut(s) 199
MnlI CCTC 2 cut(s) 166, 169
Mox20I TGGCCA 1 cut(s) 279
MroXI GAANNNNTTC 1 cut(s) 501
MscI TGGCCA 1 cut(s) 279
MseI TTAA 1 cut(s) 686
Msp20I TGGCCA 1 cut(s) 279
MspA1I CMGCKG 3 cut(s) 132, 458, 513
MspI CCGG 3 cut(s) 13, 78, 198
MspR9I CCNGG 3 cut(s) 14, 78, 276
MunI CAATTG 1 cut(s) 628
MvaI CCWGG 1 cut(s) 276
MvnI CGCG 5 cut(s) 62, 319, 473, 760, 762
MwoI GCNNNNNNNGC 1 cut(s) 479
NarI GGCGCC 1 cut(s) 638
NciI CCSGG 2 cut(s) 14, 78
NdeII GATC 2 cut(s) 63, 436
NheI GCTAGC 1 cut(s) 779
NlaIII CATG 3 cut(s) 112, 334, 376
NlaIV GGNNCC 2 cut(s) 549, 639
NmuCI GTSAC 2 cut(s) 200, 703
NruI TCGCGA 1 cut(s) 62
NspI RCATGY 1 cut(s) 112
NspV TTCGAA 1 cut(s) 505
PauI GCGCGC 1 cut(s) 760
PceI AGGCCT 1 cut(s) 488
PciI ACATGT 1 cut(s) 108
PdmI GAANNNNTTC 1 cut(s) 501
PfeI GAWTC 4 cut(s) 17, 307, 461, 557
PflMI CCANNNNNTGG 1 cut(s) 286
PfoI TCCNGGA 1 cut(s) 76
PkrI GCNGC 2 cut(s) 642, 679
PleI GAGTC 3 cut(s) 235, 307, 347
PluTI GGCGCC 1 cut(s) 641
PpsI GAGTC 3 cut(s) 235, 307, 347
Ppu21I YACGTR 1 cut(s) 745
PscI ACATGT 1 cut(s) 108
PsiI TTATAA 2 cut(s) 414, 651
Psp1406I AACGTT 1 cut(s) 697
Psp6I CCWGG 1 cut(s) 274
PspGI CCWGG 1 cut(s) 274
PspN4I GGNNCC 2 cut(s) 549, 639
PspPI GGNCC 1 cut(s) 547
PstI CTGCAG 1 cut(s) 729
PteI GCGCGC 1 cut(s) 760
PvuII CAGCTG 1 cut(s) 458
RruI TCGCGA 1 cut(s) 62
RsaI GTAC 4 cut(s) 113, 367, 743, 747
RsaNI GTAC 4 cut(s) 112, 366, 742, 746
SalI GTCGAC 1 cut(s) 350
SaqAI TTAA 1 cut(s) 686
SatI GCNGC 2 cut(s) 641, 678
Sau3AI GATC 2 cut(s) 63, 436
Sau96I GGNCC 1 cut(s) 547
SchI GAGTC 3 cut(s) 235, 307, 347
ScrFI CCNGG 3 cut(s) 14, 78, 276
SetI ASST 7 cut(s) 87, 450, 460, 700, 747, 781, 785
SfaNI GCATC 2 cut(s) 660, 689
SfcI CTRYAG 1 cut(s) 725
SfoI GGCGCC 1 cut(s) 639
SfuI TTCGAA 1 cut(s) 505
SinI GGWCC 1 cut(s) 547
SnaBI TACGTA 1 cut(s) 745
Sse9I AATT 9 cut(s) 116, 139, 192, 359, 393, 409, 588, 628, 646
SseBI AGGCCT 1 cut(s) 488
SsiI CCGC 7 cut(s) 132, 151, 164, 317, 406, 513, 641
SspDI GGCGCC 1 cut(s) 637
SspMI CTAG 3 cut(s) 621, 780, 784
StuI AGGCCT 1 cut(s) 488
StyD4I CCNGG 3 cut(s) 12, 76, 274
TaaI ACNGT 4 cut(s) 71, 214, 230, 547
TaiI ACGT 2 cut(s) 700, 747
TaqI TCGA 2 cut(s) 351, 505
TasI AATT 9 cut(s) 116, 139, 192, 359, 393, 409, 588, 628, 646
TatI WGTACW 1 cut(s) 111
TauI GCSGC 1 cut(s) 643
TfiI GAWTC 4 cut(s) 17, 307, 461, 557
Tru1I TTAA 1 cut(s) 686
Tru9I TTAA 1 cut(s) 686
TscAI CASTG 2 cut(s) 427, 559
TseFI GTSAC 2 cut(s) 200, 703
TseI GCWGC 1 cut(s) 677
Tsp45I GTSAC 2 cut(s) 200, 703
TspDTI ATGAA 1 cut(s) 389
TspGWI ACGGA 1 cut(s) 10
TspRI CASTG 2 cut(s) 427, 559
Van91I CCANNNNNTGG 1 cut(s) 286
VpaK11BI GGWCC 1 cut(s) 547
XapI RAATTY 2 cut(s) 116, 359
XceI RCATGY 1 cut(s) 112
XmiI GTMKAC 1 cut(s) 351
XmnI GAANNNNTTC 1 cut(s) 501
XspI CTAG 3 cut(s) 621, 780, 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.