MD10G1042600.v1.1

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004220
Physical Location & Seq
Reverse (-)
5539164 .. 5540795
1632 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1042600.v1.1.491

Sequence Viewer

Length: 861 bp
ATGGCTTCTCATCCTTTTATGGATGGAGTTAATGTTTCATATAACAACAGCACCACCAGTGCAATTGTTCAATACAACGGCAACTACAGCTCCCCATCAATCATTCCTTTTCCAACTCTTCCAGCGGAAGCCGATGAAACAGCTGCTGATAACTTCACTAAGAAAATCAGGGCCTTGGCAAGCAAAGAGCACCCTATTGATGTCCCACTAGAAATTCATTCCAGGATCTATATGACTCTTGCAATCAATGAAAGAATTTGTGCTAATTCTTCATGTGAAGGCCCGGATGGAAATGCGCTCGCTTCAAGCTTAAACAATGTCAGTTTCGTGACACCATCTGTTGATATACTGCAAGCATATTATCGGGGAAGTAACAATGGAGTTTACAGAACTAGTTTCCCAAACAGGCCGCCTAATCCTTTCAACTACACTGGAGACGTGGGTAACAACACAATATATCCGAACTTTGGGACGAGGGTGAGGATGATCAAATATGGTGCAGGAGTTGAGATCATGTTCCAAGGAACCAACATCATCGCACCTGAGAACCATCCAATGCATCTCCATGGTTTCAGCTTCTATTTGGTCGGAACTGGTTCCGGAATTTTTGACAGGAGAACATCACCCAGAACCTACAATTTGGTTGATCCCCCAGAAGTTAACACCATTGGAGTTCCAAAGAATGGATGGGCAACCATCAGATTTACAGCCGATAATCCTGGAGTATGGTTTATGCATTGTCATTTGGAAAGACATACCAGCTGGGGAATGGATACCGTGCTCATAGTGCAGAATGGAAATACAACAGAAACCAGCATTCGCCCACCGCCTGCTTATATGCCTCCTTGTTCCAAGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

287

Amino Acids

31.5

Weight (kDa)

6.49

Isoelectric Point (pI)

34.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_2 PF07731 133 - 266 8.6e-39 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 683
AccIII TCCGGA 1 cut(s) 599
AciI CCGC 3 cut(s) 125, 410, 827
AclWI GGATC 2 cut(s) 233, 641
AcsI RAATTY 3 cut(s) 213, 255, 603
AfiI CCNNNNNNNGG 2 cut(s) 467, 683
AgsI TTSAA 3 cut(s) 71, 306, 424
AhlI ACTAGT 1 cut(s) 392
AjiI CACGTC 1 cut(s) 439
AjnI CCWGG 2 cut(s) 221, 718
AluBI AGCT 5 cut(s) 90, 143, 309, 576, 762
AluI AGCT 5 cut(s) 90, 143, 309, 576, 762
Alw21I GWGCWC 2 cut(s) 192, 783
Alw26I GTCTC 1 cut(s) 429
AlwI GGATC 2 cut(s) 233, 641
AlwNI CAGNNNCTG 1 cut(s) 146
Aor13HI TCCGGA 1 cut(s) 599
AoxI GGCC 3 cut(s) 171, 280, 407
ApeKI GCWGC 1 cut(s) 143
ApoI RAATTY 3 cut(s) 213, 255, 603
Asp700I GAANNNNTTC 1 cut(s) 595
AspLEI GCGC 1 cut(s) 298
AspS9I GGNCC 2 cut(s) 171, 281
AsuC2I CCSGG 1 cut(s) 284
AsuHPI GGTGA 2 cut(s) 490, 615
Bbv12I GWGCWC 2 cut(s) 192, 783
BbvI GCAGC 1 cut(s) 130
BccI CCATC 7 cut(s) 17, 103, 281, 343, 558, 681, 704
BceAI ACGGC 1 cut(s) 94
BciT130I CCWGG 2 cut(s) 223, 720
BciVI GTATCC 1 cut(s) 766
BclI TGATCA 1 cut(s) 486
BcnI CCSGG 1 cut(s) 284
BcoDI GTCTC 1 cut(s) 429
BcuI ACTAGT 1 cut(s) 392
BfaI CTAG 2 cut(s) 209, 393
BfmI CTRYAG 1 cut(s) 85
BfuI GTATCC 1 cut(s) 766
BisI GCNGC 2 cut(s) 144, 410
BlsI GCNGC 2 cut(s) 145, 411
Bme1390I CCNGG 3 cut(s) 223, 284, 720
BmgBI CACGTC 1 cut(s) 439
BmgT120I GGNCC 2 cut(s) 171, 281
BmiI GGNNCC 2 cut(s) 526, 598
BmrFI CCNGG 3 cut(s) 223, 284, 720
BmsI GCATC 1 cut(s) 568
BpmI CTGGAG 2 cut(s) 453, 741
BpuMI CCSGG 1 cut(s) 284
BsaJI CCNNGG 3 cut(s) 174, 520, 565
BsaWI WCCGGW 1 cut(s) 599
BsaXI ACNNNNNCTCC 4 cut(s) 18, 48, 74, 104
Bsc4I CCNNNNNNNGG 2 cut(s) 467, 683
Bse1I ACTGG 3 cut(s) 57, 436, 598
BseAI TCCGGA 1 cut(s) 599
BseBI CCWGG 2 cut(s) 223, 720
BseDI CCNNGG 3 cut(s) 174, 520, 565
BseGI GGATG 6 cut(s) 10, 28, 292, 489, 550, 692
BseLI CCNNNNNNNGG 2 cut(s) 467, 683
BseMII CTCAG 1 cut(s) 534
BseNI ACTGG 3 cut(s) 57, 436, 598
BseXI GCAGC 1 cut(s) 130
BseYI CCCAGC 1 cut(s) 762
BsgI GTGCAG 2 cut(s) 519, 809
BshFI GGCC 3 cut(s) 173, 282, 409
BsiHKAI GWGCWC 2 cut(s) 192, 783
BsiSI CCGG 2 cut(s) 284, 600
BslFI GGGAC 2 cut(s) 188, 484
BslI CCNNNNNNNGG 2 cut(s) 467, 683
BsmAI GTCTC 1 cut(s) 429
BsmBI CGTCTC 1 cut(s) 429
BsmFI GGGAC 2 cut(s) 188, 484
BsmI GAATGC 1 cut(s) 816
BsnI GGCC 3 cut(s) 173, 282, 409
Bsp1286I GDGCHC 2 cut(s) 192, 783
Bsp13I TCCGGA 1 cut(s) 599
Bsp143I GATC 4 cut(s) 225, 486, 510, 646
Bsp19I CCATGG 1 cut(s) 565
BspACI CCGC 3 cut(s) 125, 410, 827
BspANI GGCC 3 cut(s) 173, 282, 409
BspCNI CTCAG 1 cut(s) 535
BspEI TCCGGA 1 cut(s) 599
BspLI GGNNCC 2 cut(s) 526, 598
BspPI GGATC 2 cut(s) 233, 641
BsrI ACTGG 3 cut(s) 57, 436, 598
BssECI CCNNGG 3 cut(s) 174, 520, 565
BssMI GATC 4 cut(s) 225, 486, 510, 646
BssT1I CCWWGG 3 cut(s) 174, 520, 565
Bst2UI CCWGG 2 cut(s) 223, 720
Bst4CI ACNGT 1 cut(s) 778
Bst6I CTCTTC 1 cut(s) 123
BstC8I GCNNGC 4 cut(s) 181, 300, 354, 831
BstDEI CTNAG 2 cut(s) 159, 543
BstDSI CCRYGG 1 cut(s) 565
BstF5I GGATG 6 cut(s) 10, 28, 292, 489, 550, 692
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 4 cut(s) 228, 489, 513, 649
BstMAI GTCTC 1 cut(s) 429
BstMBI GATC 4 cut(s) 225, 486, 510, 646
BstMWI GCNNNNNNNGC 2 cut(s) 87, 787
BstNI CCWGG 2 cut(s) 223, 720
BstSCI CCNGG 3 cut(s) 221, 282, 718
BstSFI CTRYAG 1 cut(s) 85
BstV1I GCAGC 1 cut(s) 130
BstX2I RGATCY 1 cut(s) 225
BstYI RGATCY 1 cut(s) 225
BsuI GTATCC 1 cut(s) 766
BsuRI GGCC 3 cut(s) 173, 282, 409
BtgI CCRYGG 1 cut(s) 565
BtgZI GCGATG 1 cut(s) 520
BtrI CACGTC 1 cut(s) 439
BtsCI GGATG 6 cut(s) 10, 28, 292, 489, 550, 692
BtsIMutI CAGTG 2 cut(s) 64, 429
Cac8I GCNNGC 4 cut(s) 181, 300, 354, 831
CaiI CAGNNNCTG 1 cut(s) 146
CfoI GCGC 1 cut(s) 298
Cfr13I GGNCC 2 cut(s) 171, 281
CviAII CATG 3 cut(s) 273, 514, 566
DdeI CTNAG 2 cut(s) 159, 543
DpnI GATC 4 cut(s) 227, 488, 512, 648
DpnII GATC 4 cut(s) 225, 486, 510, 646
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Eco130I CCWWGG 3 cut(s) 174, 520, 565
EcoO109I RGGNCCY 1 cut(s) 171
EcoRII CCWGG 2 cut(s) 221, 718
EcoT14I CCWWGG 3 cut(s) 174, 520, 565
EcoT22I ATGCAT 2 cut(s) 561, 738
ErhI CCWWGG 3 cut(s) 174, 520, 565
Esp3I CGTCTC 1 cut(s) 429
FaeI CATG 3 cut(s) 276, 517, 569
FaqI GGGAC 2 cut(s) 188, 484
FatI CATG 3 cut(s) 272, 513, 565
FbaI TGATCA 1 cut(s) 486
Fnu4HI GCNGC 2 cut(s) 144, 410
FokI GGATG 5 cut(s) 35, 299, 496, 537, 699
Fsp4HI GCNGC 2 cut(s) 144, 410
FspBI CTAG 2 cut(s) 209, 393
GlaI GCGC 1 cut(s) 297
GluI GCNGC 2 cut(s) 144, 410
GsaI CCCAGC 1 cut(s) 766
GsuI CTGGAG 2 cut(s) 453, 741
HaeIII GGCC 3 cut(s) 173, 282, 409
HapII CCGG 2 cut(s) 284, 600
HhaI GCGC 1 cut(s) 298
Hin1II CATG 3 cut(s) 276, 517, 569
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HincII GTYRAC 1 cut(s) 661
HindII GTYRAC 1 cut(s) 661
HindIII AAGCTT 1 cut(s) 307
HinfI GANTC 1 cut(s) 235
HpaI GTTAAC 1 cut(s) 661
HpaII CCGG 2 cut(s) 284, 600
HphI GGTGA 2 cut(s) 490, 615
Hpy166II GTNNAC 2 cut(s) 385, 661
Hpy188I TCNGA 3 cut(s) 462, 590, 701
Hpy188III TCNNGA 2 cut(s) 328, 600
Hpy8I GTNNAC 2 cut(s) 385, 661
HpyAV CCTTC 1 cut(s) 272
HpyCH4III ACNGT 1 cut(s) 778
HpyCH4IV ACGT 1 cut(s) 438
HpyCH4V TGCA 7 cut(s) 62, 242, 352, 500, 559, 736, 790
HpyF10VI GCNNNNNNNGC 2 cut(s) 87, 787
HpyF3I CTNAG 2 cut(s) 159, 543
HpySE526I ACGT 1 cut(s) 438
Hsp92II CATG 3 cut(s) 276, 517, 569
HspAI GCGC 1 cut(s) 296
Kpn2I TCCGGA 1 cut(s) 599
Ksp22I TGATCA 1 cut(s) 486
KspAI GTTAAC 1 cut(s) 661
Kzo9I GATC 4 cut(s) 225, 486, 510, 646
LmnI GCTCC 1 cut(s) 95
Lsp1109I GCAGC 1 cut(s) 130
LweI GCATC 1 cut(s) 568
MaeI CTAG 2 cut(s) 209, 393
MaeII ACGT 1 cut(s) 438
MaeIII GTNAC 3 cut(s) 328, 371, 443
MalI GATC 4 cut(s) 227, 488, 512, 648
MboI GATC 4 cut(s) 225, 486, 510, 646
MboII GAAGA 2 cut(s) 110, 261
MfeI CAATTG 1 cut(s) 63
MflI RGATCY 1 cut(s) 225
MhlI GDGCHC 2 cut(s) 192, 783
MluCI AATT 6 cut(s) 63, 213, 255, 265, 603, 637
MlyI GAGTC 1 cut(s) 229
MmeI TCCRAC 2 cut(s) 137, 568
MnlI CCTC 3 cut(s) 468, 474, 852
Mph1103I ATGCAT 2 cut(s) 561, 738
MroI TCCGGA 1 cut(s) 599
MroXI GAANNNNTTC 1 cut(s) 595
MseI TTAA 4 cut(s) 30, 311, 660, 859
MslI CAYNNNNRTG 1 cut(s) 564
MspA1I CMGCKG 3 cut(s) 125, 143, 762
MspI CCGG 2 cut(s) 284, 600
MspR9I CCNGG 3 cut(s) 223, 284, 720
MunI CAATTG 1 cut(s) 63
Mva1269I GAATGC 1 cut(s) 816
MvaI CCWGG 2 cut(s) 223, 720
MwoI GCNNNNNNNGC 2 cut(s) 87, 787
NciI CCSGG 1 cut(s) 284
NcoI CCATGG 1 cut(s) 565
NdeII GATC 4 cut(s) 225, 486, 510, 646
NlaIII CATG 3 cut(s) 276, 517, 569
NlaIV GGNNCC 2 cut(s) 526, 598
NmuCI GTSAC 1 cut(s) 328
NsiI ATGCAT 2 cut(s) 561, 738
PctI GAATGC 1 cut(s) 816
PdmI GAANNNNTTC 1 cut(s) 595
PflMI CCANNNNNTGG 1 cut(s) 683
PfoI TCCNGGA 2 cut(s) 221, 718
PkrI GCNGC 2 cut(s) 145, 411
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
Psp6I CCWGG 2 cut(s) 221, 718
PspFI CCCAGC 1 cut(s) 762
PspGI CCWGG 2 cut(s) 221, 718
PspN4I GGNNCC 2 cut(s) 526, 598
PspPI GGNCC 2 cut(s) 171, 281
PstNI CAGNNNCTG 1 cut(s) 146
PsuI RGATCY 1 cut(s) 225
PvuII CAGCTG 2 cut(s) 143, 762
RseI CAYNNNNRTG 1 cut(s) 564
SaqAI TTAA 4 cut(s) 30, 311, 660, 859
SatI GCNGC 2 cut(s) 144, 410
Sau3AI GATC 4 cut(s) 225, 486, 510, 646
Sau96I GGNCC 2 cut(s) 171, 281
SchI GAGTC 1 cut(s) 229
ScrFI CCNGG 3 cut(s) 223, 284, 720
SduI GDGCHC 2 cut(s) 192, 783
SetI ASST 8 cut(s) 92, 145, 311, 441, 544, 578, 635, 764
SfaNI GCATC 1 cut(s) 568
SfcI CTRYAG 1 cut(s) 85
SmiMI CAYNNNNRTG 1 cut(s) 564
SpeI ACTAGT 1 cut(s) 392
Sse9I AATT 6 cut(s) 63, 213, 255, 265, 603, 637
SsiI CCGC 3 cut(s) 125, 410, 827
SspMI CTAG 2 cut(s) 209, 393
StyD4I CCNGG 3 cut(s) 221, 282, 718
StyI CCWWGG 3 cut(s) 174, 520, 565
TaaI ACNGT 1 cut(s) 778
TaiI ACGT 1 cut(s) 441
TasI AATT 6 cut(s) 63, 213, 255, 265, 603, 637
TauI GCSGC 1 cut(s) 412
Tru1I TTAA 4 cut(s) 30, 311, 660, 859
Tru9I TTAA 4 cut(s) 30, 311, 660, 859
TscAI CASTG 2 cut(s) 64, 436
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 1 cut(s) 143
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 5 cut(s) 27, 150, 206, 261, 264
TspRI CASTG 2 cut(s) 64, 436
Van91I CCANNNNNTGG 1 cut(s) 683
XapI RAATTY 3 cut(s) 213, 255, 603
XcmI CCANNNNNNNNNTGG 2 cut(s) 684, 766
XmnI GAANNNNTTC 1 cut(s) 595
XspI CTAG 2 cut(s) 209, 393
Zsp2I ATGCAT 2 cut(s) 561, 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.