MD00G1193000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0004912
Physical Location & Seq
Forward (+)
46700741 .. 46702541
1801 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1193000.v1.1.491

Sequence Viewer

Length: 513 bp
ATGTCGCTGGTAGAATATCTGGAAGAGATGGACCAGAGCATGAAGCAAATGCTGAAGCTGATCAAAGAAGATGGTGATTCTTTGCCCAACAAAGACGAAATGTTTTTGCAGAAGAACCCGGAATTAGTTCAGGAATTTCACCGGATGTATCGTTCACTGGCCGGATGCTATCAACACTTGAACAAAGCACATAAGGGGTTTGGAGATTCTGAATATGGTTTCAACCAAGGCTCTCCTCTGCTTACTCCCGATGCTAAACATGGTTTGCGGAAATCCGACGATCAAGTTGTTGGTTTTGATATATCACTAAGCTCTGATGGTTCTAGTCCAGCTCTTTCTATAAAGAATGGCGCTGAATCATCTTCCTCTTCATCATTGGGATCCGAGTCAGATTCTCCTAATTCCCCGGTCAGCAATTACTTGGTTCCACCTCCGAGTGCTGATTTCGACAGTCAGGGATGGCAGCAGAAGATTGCTGAGCTTGAAAGAGAAGCTCCAGGTAAAGGAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2 KIP1

Protein Analysis

171

Amino Acids

18.63

Weight (kDa)

4.78

Isoelectric Point (pI)

62.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 3 - 57 9.8e-15 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 268
AclWI GGATC 2 cut(s) 375, 388
AcoI YGGCCR 1 cut(s) 159
AcsI RAATTY 1 cut(s) 134
AcuI CTGAAG 1 cut(s) 74
AfiI CCNNNNNNNGG 1 cut(s) 503
AgsI TTSAA 3 cut(s) 181, 223, 485
AjnI CCWGG 1 cut(s) 496
AloI GAACNNNNNNTCC 2 cut(s) 136, 168
AluBI AGCT 5 cut(s) 58, 312, 332, 481, 494
AluI AGCT 5 cut(s) 58, 312, 332, 481, 494
AlwI GGATC 2 cut(s) 375, 388
AoxI GGCC 1 cut(s) 159
ApeKI GCWGC 1 cut(s) 463
ApoI RAATTY 1 cut(s) 134
Asp700I GAANNNNTTC 1 cut(s) 126
AspLEI GCGC 1 cut(s) 353
AspS9I GGNCC 1 cut(s) 31
AsuC2I CCSGG 2 cut(s) 119, 407
AsuHPI GGTGA 2 cut(s) 86, 131
AvaII GGWCC 1 cut(s) 31
BamHI GGATCC 1 cut(s) 380
BbvI GCAGC 1 cut(s) 475
BccI CCATC 4 cut(s) 22, 65, 311, 453
BciT130I CCWGG 1 cut(s) 498
BclI TGATCA 1 cut(s) 60
BcnI CCSGG 2 cut(s) 119, 407
BfaI CTAG 1 cut(s) 324
BfoI RGCGCY 1 cut(s) 354
BisI GCNGC 1 cut(s) 464
BlpI GCTNAGC 1 cut(s) 477
BlsI GCNGC 1 cut(s) 465
Bme1390I CCNGG 3 cut(s) 119, 407, 498
Bme18I GGWCC 1 cut(s) 31
BmgT120I GGNCC 1 cut(s) 31
BmiI GGNNCC 2 cut(s) 382, 426
BmrFI CCNGG 3 cut(s) 119, 407, 498
BmsI GCATC 2 cut(s) 155, 241
BpmI CTGGAG 1 cut(s) 480
Bpu1102I GCTNAGC 1 cut(s) 477
BpuMI CCSGG 2 cut(s) 119, 407
BsaJI CCNNGG 2 cut(s) 226, 405
BsaWI WCCGGW 1 cut(s) 141
Bsc4I CCNNNNNNNGG 1 cut(s) 503
Bse1I ACTGG 1 cut(s) 162
BseBI CCWGG 1 cut(s) 498
BseDI CCNNGG 2 cut(s) 226, 405
BseGI GGATG 3 cut(s) 150, 170, 464
BseLI CCNNNNNNNGG 1 cut(s) 503
BseMII CTCAG 1 cut(s) 468
BseNI ACTGG 1 cut(s) 162
BseRI GAGGAG 1 cut(s) 225
BseXI GCAGC 1 cut(s) 475
BshFI GGCC 1 cut(s) 161
BsiSI CCGG 4 cut(s) 119, 142, 162, 407
BslI CCNNNNNNNGG 1 cut(s) 503
BsnI GGCC 1 cut(s) 161
Bsp143I GATC 3 cut(s) 60, 280, 380
Bsp1720I GCTNAGC 1 cut(s) 477
BspACI CCGC 1 cut(s) 268
BspANI GGCC 1 cut(s) 161
BspCNI CTCAG 1 cut(s) 469
BspLI GGNNCC 2 cut(s) 382, 426
BspPI GGATC 2 cut(s) 375, 388
BsrI ACTGG 1 cut(s) 162
BssECI CCNNGG 2 cut(s) 226, 405
BssMI GATC 3 cut(s) 60, 280, 380
BssT1I CCWWGG 1 cut(s) 226
Bst2UI CCWGG 1 cut(s) 498
Bst4CI ACNGT 1 cut(s) 452
Bst6I CTCTTC 2 cut(s) 18, 373
BstDEI CTNAG 2 cut(s) 308, 477
BstF5I GGATG 3 cut(s) 150, 170, 464
BstH2I RGCGCY 1 cut(s) 354
BstHHI GCGC 1 cut(s) 353
BstKTI GATC 3 cut(s) 63, 283, 383
BstMBI GATC 3 cut(s) 60, 280, 380
BstNI CCWGG 1 cut(s) 498
BstSCI CCNGG 3 cut(s) 117, 405, 496
BstV1I GCAGC 1 cut(s) 475
BstX2I RGATCY 1 cut(s) 380
BstYI RGATCY 1 cut(s) 380
BsuRI GGCC 1 cut(s) 161
BtsCI GGATG 3 cut(s) 150, 170, 464
BtsIMutI CAGTG 1 cut(s) 155
CfoI GCGC 1 cut(s) 353
Cfr13I GGNCC 1 cut(s) 31
CviAII CATG 2 cut(s) 40, 260
CviJI RGCY 7 cut(s) 58, 161, 231, 312, 332, 481, 494
CviKI_1 RGCY 7 cut(s) 58, 161, 231, 312, 332, 481, 494
DdeI CTNAG 2 cut(s) 308, 477
DpnI GATC 3 cut(s) 62, 282, 382
DpnII GATC 3 cut(s) 60, 280, 380
EaeI YGGCCR 1 cut(s) 159
Eam1104I CTCTTC 2 cut(s) 18, 373
EarI CTCTTC 2 cut(s) 18, 373
Eco130I CCWWGG 1 cut(s) 226
Eco47I GGWCC 1 cut(s) 31
Eco57I CTGAAG 1 cut(s) 74
EcoRII CCWGG 1 cut(s) 496
EcoT14I CCWWGG 1 cut(s) 226
ErhI CCWWGG 1 cut(s) 226
FaeI CATG 2 cut(s) 43, 263
FaiI YATR 6 cut(s) 41, 192, 216, 261, 302, 341
FatI CATG 2 cut(s) 39, 259
FbaI TGATCA 1 cut(s) 60
Fnu4HI GCNGC 1 cut(s) 464
FokI GGATG 3 cut(s) 157, 177, 471
Fsp4HI GCNGC 1 cut(s) 464
FspBI CTAG 1 cut(s) 324
GlaI GCGC 1 cut(s) 352
GluI GCNGC 1 cut(s) 464
GsuI CTGGAG 1 cut(s) 480
HaeII RGCGCY 1 cut(s) 354
HaeIII GGCC 1 cut(s) 161
HapII CCGG 4 cut(s) 119, 142, 162, 407
HhaI GCGC 1 cut(s) 353
Hin1II CATG 2 cut(s) 43, 263
Hin6I GCGC 1 cut(s) 351
HinP1I GCGC 1 cut(s) 351
HinfI GANTC 6 cut(s) 77, 206, 356, 386, 392, 507
HpaII CCGG 4 cut(s) 119, 142, 162, 407
HphI GGTGA 2 cut(s) 86, 131
Hpy166II GTNNAC 1 cut(s) 155
Hpy188I TCNGA 7 cut(s) 211, 277, 316, 385, 391, 435, 512
Hpy188III TCNNGA 3 cut(s) 20, 131, 248
Hpy8I GTNNAC 1 cut(s) 155
Hpy99I CGWCG 1 cut(s) 281
HpyCH4III ACNGT 1 cut(s) 452
HpyCH4V TGCA 1 cut(s) 109
HpyF3I CTNAG 2 cut(s) 308, 477
Hsp92II CATG 2 cut(s) 43, 263
HspAI GCGC 1 cut(s) 351
Ksp22I TGATCA 1 cut(s) 60
Kzo9I GATC 3 cut(s) 60, 280, 380
LmnI GCTCC 1 cut(s) 499
Lsp1109I GCAGC 1 cut(s) 475
LweI GCATC 2 cut(s) 155, 241
MaeI CTAG 1 cut(s) 324
MalI GATC 3 cut(s) 62, 282, 382
MboI GATC 3 cut(s) 60, 280, 380
MboII GAAGA 6 cut(s) 35, 80, 124, 354, 360, 481
MflI RGATCY 1 cut(s) 380
MluCI AATT 4 cut(s) 122, 134, 400, 415
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 1 cut(s) 300
MnlI CCTC 3 cut(s) 246, 376, 441
MroXI GAANNNNTTC 1 cut(s) 126
MspI CCGG 4 cut(s) 119, 142, 162, 407
MspR9I CCNGG 3 cut(s) 119, 407, 498
MvaI CCWGG 1 cut(s) 498
NciI CCSGG 2 cut(s) 119, 407
NdeII GATC 3 cut(s) 60, 280, 380
NlaIII CATG 2 cut(s) 43, 263
NlaIV GGNNCC 2 cut(s) 382, 426
PdmI GAANNNNTTC 1 cut(s) 126
PfeI GAWTC 4 cut(s) 77, 206, 356, 392
PkrI GCNGC 1 cut(s) 465
PleI GAGTC 1 cut(s) 394
PpsI GAGTC 1 cut(s) 394
Psp6I CCWGG 1 cut(s) 496
PspGI CCWGG 1 cut(s) 496
PspN4I GGNNCC 2 cut(s) 382, 426
PspPI GGNCC 1 cut(s) 31
PsuI RGATCY 1 cut(s) 380
SatI GCNGC 1 cut(s) 464
Sau3AI GATC 3 cut(s) 60, 280, 380
Sau96I GGNCC 1 cut(s) 31
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 3 cut(s) 119, 407, 498
SetI ASST 7 cut(s) 60, 314, 334, 433, 483, 496, 502
SfaNI GCATC 2 cut(s) 155, 241
SinI GGWCC 1 cut(s) 31
Sse9I AATT 4 cut(s) 122, 134, 400, 415
SsiI CCGC 1 cut(s) 268
SspMI CTAG 1 cut(s) 324
StyD4I CCNGG 3 cut(s) 117, 405, 496
StyI CCWWGG 1 cut(s) 226
TaaI ACNGT 1 cut(s) 452
TaqI TCGA 1 cut(s) 447
TasI AATT 4 cut(s) 122, 134, 400, 415
TfiI GAWTC 4 cut(s) 77, 206, 356, 392
TscAI CASTG 1 cut(s) 162
TseI GCWGC 1 cut(s) 463
TspDTI ATGAA 2 cut(s) 56, 360
TspRI CASTG 1 cut(s) 162
VpaK11BI GGWCC 1 cut(s) 31
XapI RAATTY 1 cut(s) 134
XmnI GAANNNNTTC 1 cut(s) 126
XspI CTAG 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.