MD01G1170000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004912
Physical Location & Seq
Reverse (-)
27356953 .. 27359682
2730 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1170000.v1.1.491

Sequence Viewer

Length: 1779 bp
ATGTATCTATTTTTCATTTTGAAAAAAAGCCGCTTTTACCTCAAAACGCAAAAGCACCGATTAATCGCTTCGCTTATCCTTCTGATTCGGGCAGGAATTGGATCTGGAGCTCTCTCTCTCTCTCTCTCTAAGTCTCTTCTCCTCTGTCGCAGCAAAAGGTCAGTGACAATGGTTGATATGGAGAGAACAGAACTGAAGGAGTCAGATTCAACTGGACTGGACAATGGTATCACCCCAGATCGTTCTACTTCTATGTGGCTGGTAGAAAATCTTGAAGAGATGGACCAGAGGATTAAGCAAATGGTGAAGCTGATCAAAGAAGATGGCGATTCTTTGCCAAAAAATGACGAAGCGTTTTATCAGAAGAAGCCAGAATTGGTTGAGGAATTTCACAGAATGTATCGGTCGCTGGCTGGATGCTACGAACACTTGACCAAAGAAGAACATAAGGGGTTGGGAGATTCTGAATATGGTTTCGACCAAGGCTCTCCTCTGTTGACTCCTGATGCTAAACATGGTCTGCACAAATCCGGCCATCAAGTTGTTGGGTTAGGTATATCACCAAGCTCAGATGGTTCTAGTCCAGCTCTTTCTTTAAAGAATGGCAGCGAATCATCTTCCTCGTCATCATTGGGATCCGAGTCAGAATCTCTTAACTCCCCCGTCAGCAATTACTTGGTTCCACCTCCGAACGATGATTTTGACAGCCAGGGATGGCAGCAGAAGATTACTGAGCTTGAAACCGAACTTTCTAGCGTGAGAGAGAAGCTCCAAATGAGGGAGGCTGATCTTAAAATGGAGAAAATGCGGGTGTTTGAGCTGCAAAATCAGACAGTTGAGTTGGAGAGTCTGGCATCAGACAGAGAAAATGAAATTAGGAGATTGTTGGGAGACTTGGAAGTGACTAAGGAAAGGCGTAAGGGGTTGGACAAAGAGATTGTAAAGTTGAAGGATGAACATGCCCATAGAATTTCCGAAGGTGCTCATCAAATGCAAGGTCTGGAAGCCCAACTTGACTTGGAGAGAAAGCATGTTTCGGAGCTGGAGGAGAGGATAGTGAGGTACAATGGTGATATACTCGGCCTTGATCTTGAGGTAATGCAACTGAAGAGTGCATTGCATGATGCACAGGAACAATTCTCTCTTGAGAAAGCAAACCTGCAGGCTGACATTTCAAGTTTTTCAGAGAAACAAGTCCTTTTGGACACAAGAGTCGAAGAATTGAGCTTCAAAAGCAAGAGTTTGGACGACGAAATAAGGCAATGTGAAGCGGATAAGATGGAAATGGAAAGGCTGCACGTTGTCCGCGAGATGGCATTGCAAGATGAAATAAGCCGCCTGAAAGTAGAAGTTGCTGACAGAAATGGACATGTGGAAGCTGTAAATAAAGACTTCGACAGGTTTAAACTGAAATATGATATGCTGATGGCGGAGAAAGATGAGCTCAATGCTAGGGCTCAAACGCTTATGGCGAATGTGAGTTCCCGCGACAATCAGATTCAGGAAATGGCGGGACATCTTAGTCTGTTACGAACAGAACACGAGGGTCTGATATTTGGATCCGAATGTGCACATAGACTAGTAGATGAGTTGAAATCGAGAGTGGAGGAGTTGCAGCAAGAGGTGAATAGGCAGAGTGTTGTGATCTCGGATGGGGCTGAGGAGAAAAGAGAGGTGATCCGGCAGCTTTGCTTCTCGCTGGAGCACTACAGGAGCGGATACCAAGAGCTTCGTCAAGCATTTACCGGGCACAGGAGACGGGTGGTTGCAGCTGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2 KIP1

Protein Analysis

593

Amino Acids

67.52

Weight (kDa)

5.42

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 82 - 141 1.5e-18 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1211, 1521
Acc36I ACCTGC 1 cut(s) 1167
AccBSI CCGCTC 1 cut(s) 1716
AccII CGCG 2 cut(s) 1308, 1488
AciI CCGC 9 cut(s) 31, 808, 1271, 1306, 1336, 1430, 1486, 1511, 1716
AclWI GGATC 6 cut(s) 109, 630, 643, 1554, 1567, 1672
AcoI YGGCCR 1 cut(s) 532
AcsI RAATTY 2 cut(s) 386, 969
AcuI CTGAAG 2 cut(s) 215, 1127
AfaI GTAC 1 cut(s) 1064
AfiI CCNNNNNNNGG 3 cut(s) 778, 1312, 1752
AflIII ACRYGT 1 cut(s) 1369
AgsI TTSAA 8 cut(s) 22, 210, 275, 740, 949, 1176, 1231, 1594
AhlI ACTAGT 1 cut(s) 1579
AjnI CCWGG 1 cut(s) 708
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
Alw21I GWGCWC 5 cut(s) 112, 985, 1446, 1573, 1707
Alw26I GTCTC 3 cut(s) 138, 885, 1750
Alw44I GTGCAC 1 cut(s) 1569
AlwI GGATC 6 cut(s) 109, 630, 643, 1554, 1567, 1672
AoxI GGCC 2 cut(s) 532, 1081
ApaLI GTGCAC 1 cut(s) 1569
ApeKI GCWGC 9 cut(s) 150, 606, 718, 820, 1294, 1615, 1684, 1769, 1772
ApoI RAATTY 2 cut(s) 386, 969
ArsI GACNNNNNNTTYG 2 cut(s) 982, 1014
AseI ATTAAT 1 cut(s) 62
AspS9I GGNCC 1 cut(s) 283
AsuC2I CCSGG 1 cut(s) 1747
AsuHPI GGTGA 6 cut(s) 223, 316, 552, 1082, 1636, 1687
AvaII GGWCC 1 cut(s) 283
BaeGI GKGCMC 2 cut(s) 1573, 1752
BaeI ACNNNNGTAYC 4 cut(s) 211, 244, 1054, 1087
BamHI GGATCC 2 cut(s) 635, 1559
BanII GRGCYC 3 cut(s) 112, 1446, 1459
BauI CACGAG 1 cut(s) 1541
Bbv12I GWGCWC 5 cut(s) 112, 985, 1446, 1573, 1707
BbvCI CCTCAGC 1 cut(s) 1659
BbvI GCAGC 8 cut(s) 162, 618, 730, 807, 1281, 1627, 1696, 1759
BccI CCATC 9 cut(s) 274, 317, 543, 566, 708, 1273, 1306, 1420, 1646
BciT130I CCWGG 1 cut(s) 710
BciVI GTATCC 1 cut(s) 1712
BclI TGATCA 1 cut(s) 312
BcnI CCSGG 1 cut(s) 1747
BcoDI GTCTC 3 cut(s) 138, 885, 1750
BcuI ACTAGT 1 cut(s) 1579
BfaI CTAG 4 cut(s) 579, 753, 1452, 1580
BfmI CTRYAG 2 cut(s) 1160, 1708
BfuAI ACCTGC 1 cut(s) 1167
BfuI GTATCC 1 cut(s) 1712
Bme1390I CCNGG 2 cut(s) 710, 1747
Bme18I GGWCC 1 cut(s) 283
BmgT120I GGNCC 1 cut(s) 283
BmiI GGNNCC 3 cut(s) 637, 681, 1561
BmrFI CCNGG 2 cut(s) 710, 1747
BmsI GCATC 4 cut(s) 407, 496, 863, 1114
BplI GAGNNNNNCTC 2 cut(s) 753, 785
BpmI CTGGAG 3 cut(s) 126, 1064, 1721
Bpu10I CCTNAGC 1 cut(s) 1659
BpuEI CTTGAG 2 cut(s) 1112, 1166
BpuMI CCSGG 1 cut(s) 1747
BsaJI CCNNGG 2 cut(s) 481, 709
Bsc4I CCNNNNNNNGG 3 cut(s) 778, 1312, 1752
Bse1I ACTGG 2 cut(s) 217, 222
Bse3DI GCAATG 3 cut(s) 1115, 1268, 1316
BseBI CCWGG 1 cut(s) 710
BseDI CCNNGG 2 cut(s) 481, 709
BseGI GGATG 4 cut(s) 422, 719, 958, 1657
BseLI CCNNNNNNNGG 3 cut(s) 778, 1312, 1752
BseMI GCAATG 3 cut(s) 1115, 1268, 1316
BseMII CTCAG 3 cut(s) 582, 723, 1650
BseNI ACTGG 2 cut(s) 217, 222
BseRI GAGGAG 5 cut(s) 131, 480, 1061, 1622, 1676
BseSI GKGCMC 2 cut(s) 1573, 1752
BseXI GCAGC 8 cut(s) 162, 618, 730, 807, 1281, 1627, 1696, 1759
BsgI GTGCAG 2 cut(s) 506, 1280
Bsh1236I CGCG 2 cut(s) 1308, 1488
Bsh1285I CGRYCG 1 cut(s) 407
BshFI GGCC 2 cut(s) 534, 1083
BsiEI CGRYCG 1 cut(s) 407
BsiHKAI GWGCWC 5 cut(s) 112, 985, 1446, 1573, 1707
BsiSI CCGG 3 cut(s) 531, 1681, 1746
BslFI GGGAC 1 cut(s) 1527
BslI CCNNNNNNNGG 3 cut(s) 778, 1312, 1752
BsmAI GTCTC 3 cut(s) 138, 885, 1750
BsmBI CGTCTC 1 cut(s) 1750
BsmFI GGGAC 1 cut(s) 1527
BsnI GGCC 2 cut(s) 534, 1083
Bsp1286I GDGCHC 7 cut(s) 112, 985, 1446, 1459, 1573, 1707, 1752
Bsp143I GATC 9 cut(s) 101, 238, 312, 635, 787, 1087, 1559, 1644, 1677
BspACI CCGC 9 cut(s) 31, 808, 1271, 1306, 1336, 1430, 1486, 1511, 1716
BspANI GGCC 2 cut(s) 534, 1083
BspCNI CTCAG 3 cut(s) 581, 724, 1651
BspFNI CGCG 2 cut(s) 1308, 1488
BspLI GGNNCC 3 cut(s) 637, 681, 1561
BspMAI CTGCAG 1 cut(s) 1164
BspMI ACCTGC 1 cut(s) 1167
BspPI GGATC 6 cut(s) 109, 630, 643, 1554, 1567, 1672
BsrBI CCGCTC 1 cut(s) 1716
BsrDI GCAATG 3 cut(s) 1115, 1268, 1316
BsrI ACTGG 2 cut(s) 217, 222
BssECI CCNNGG 2 cut(s) 481, 709
BssMI GATC 9 cut(s) 101, 238, 312, 635, 787, 1087, 1559, 1644, 1677
BssSI CACGAG 1 cut(s) 1541
BssT1I CCWWGG 1 cut(s) 481
Bst2BI CACGAG 1 cut(s) 1541
Bst2UI CCWGG 1 cut(s) 710
Bst4CI ACNGT 1 cut(s) 835
Bst6I CTCTTC 3 cut(s) 141, 270, 1103
BstC8I GCNNGC 2 cut(s) 411, 1164
BstDEI CTNAG 6 cut(s) 129, 568, 732, 906, 1520, 1659
BstF5I GGATG 4 cut(s) 422, 719, 958, 1657
BstFNI CGCG 2 cut(s) 1308, 1488
BstKTI GATC 9 cut(s) 104, 241, 315, 638, 790, 1090, 1562, 1647, 1680
BstMAI GTCTC 3 cut(s) 138, 885, 1750
BstMBI GATC 9 cut(s) 101, 238, 312, 635, 787, 1087, 1559, 1644, 1677
BstMCI CGRYCG 1 cut(s) 407
BstMWI GCNNNNNNNGC 1 cut(s) 1233
BstNI CCWGG 1 cut(s) 710
BstNSI RCATGY 3 cut(s) 962, 1034, 1373
BstSCI CCNGG 2 cut(s) 708, 1745
BstSFI CTRYAG 2 cut(s) 1160, 1708
BstSLI GKGCMC 2 cut(s) 1573, 1752
BstUI CGCG 2 cut(s) 1308, 1488
BstV1I GCAGC 8 cut(s) 162, 618, 730, 807, 1281, 1627, 1696, 1759
BstX2I RGATCY 3 cut(s) 101, 635, 1559
BstYI RGATCY 3 cut(s) 101, 635, 1559
BsuI GTATCC 1 cut(s) 1712
BsuRI GGCC 2 cut(s) 534, 1083
BtsCI GGATG 4 cut(s) 422, 719, 958, 1657
BtsIMutI CAGTG 1 cut(s) 168
BveI ACCTGC 1 cut(s) 1167
Cac8I GCNNGC 2 cut(s) 411, 1164
Cfr13I GGNCC 1 cut(s) 283
Csp6I GTAC 1 cut(s) 1063
CviAII CATG 5 cut(s) 515, 959, 1031, 1121, 1370
CviQI GTAC 1 cut(s) 1063
DdeI CTNAG 6 cut(s) 129, 568, 732, 906, 1520, 1659
DpnI GATC 9 cut(s) 103, 240, 314, 637, 789, 1089, 1561, 1646, 1679
DpnII GATC 9 cut(s) 101, 238, 312, 635, 787, 1087, 1559, 1644, 1677
DraI TTTAAA 2 cut(s) 597, 1405
DrdI GACNNNNNNGTC 2 cut(s) 1211, 1521
DseDI GACNNNNNNGTC 2 cut(s) 1211, 1521
EaeI YGGCCR 1 cut(s) 532
Eam1104I CTCTTC 3 cut(s) 141, 270, 1103
EarI CTCTTC 3 cut(s) 141, 270, 1103
EciI GGCGGA 1 cut(s) 1445
Ecl136II GAGCTC 2 cut(s) 110, 1444
Eco130I CCWWGG 1 cut(s) 481
Eco24I GRGCYC 3 cut(s) 112, 1446, 1459
Eco47I GGWCC 1 cut(s) 283
Eco53kI GAGCTC 2 cut(s) 110, 1444
Eco57I CTGAAG 2 cut(s) 215, 1127
EcoICRI GAGCTC 2 cut(s) 110, 1444
EcoRII CCWGG 1 cut(s) 708
EcoT14I CCWWGG 1 cut(s) 481
EcoT38I GRGCYC 3 cut(s) 112, 1446, 1459
ErhI CCWWGG 1 cut(s) 481
Esp3I CGTCTC 1 cut(s) 1750
FaeI CATG 5 cut(s) 518, 962, 1034, 1124, 1373
FalI AAGNNNNNCTT 2 cut(s) 996, 1028
FaqI GGGAC 1 cut(s) 1527
FatI CATG 5 cut(s) 514, 958, 1030, 1120, 1369
FauI CCCGC 3 cut(s) 801, 1493, 1504
FbaI TGATCA 1 cut(s) 312
FokI GGATG 4 cut(s) 429, 726, 965, 1664
FriOI GRGCYC 3 cut(s) 112, 1446, 1459
FspBI CTAG 4 cut(s) 579, 753, 1452, 1580
GsuI CTGGAG 3 cut(s) 126, 1064, 1721
HaeIII GGCC 2 cut(s) 534, 1083
HapII CCGG 3 cut(s) 531, 1681, 1746
Hin1II CATG 5 cut(s) 518, 962, 1034, 1124, 1373
HincII GTYRAC 1 cut(s) 498
HindII GTYRAC 1 cut(s) 498
HpaII CCGG 3 cut(s) 531, 1681, 1746
HphI GGTGA 6 cut(s) 223, 316, 552, 1082, 1636, 1687
Hpy166II GTNNAC 2 cut(s) 498, 1571
Hpy188III TCNNGA 8 cut(s) 105, 272, 503, 1001, 1091, 1145, 1502, 1599
Hpy8I GTNNAC 2 cut(s) 498, 1571
Hpy99I CGWCG 1 cut(s) 1253
HpyAV CCTTC 4 cut(s) 89, 190, 943, 971
HpyCH4III ACNGT 1 cut(s) 835
HpyCH4IV ACGT 1 cut(s) 1299
HpyF10VI GCNNNNNNNGC 1 cut(s) 1233
HpyF3I CTNAG 6 cut(s) 129, 568, 732, 906, 1520, 1659
HpySE526I ACGT 1 cut(s) 1299
Hsp92II CATG 5 cut(s) 518, 962, 1034, 1124, 1373
Ksp22I TGATCA 1 cut(s) 312
Kzo9I GATC 9 cut(s) 101, 238, 312, 635, 787, 1087, 1559, 1644, 1677
LmnI GCTCC 5 cut(s) 107, 774, 1039, 1702, 1713
Lsp1109I GCAGC 8 cut(s) 162, 618, 730, 807, 1281, 1627, 1696, 1759
LweI GCATC 4 cut(s) 407, 496, 863, 1114
MaeI CTAG 4 cut(s) 579, 753, 1452, 1580
MaeII ACGT 1 cut(s) 1299
MaeIII GTNAC 3 cut(s) 163, 901, 1527
MalI GATC 9 cut(s) 103, 240, 314, 637, 789, 1089, 1561, 1646, 1679
MbiI CCGCTC 1 cut(s) 1716
MboI GATC 9 cut(s) 101, 238, 312, 635, 787, 1087, 1559, 1644, 1677
MboII GAAGA 9 cut(s) 128, 287, 332, 376, 452, 609, 736, 1120, 1229
MflI RGATCY 3 cut(s) 101, 635, 1559
MhlI GDGCHC 7 cut(s) 112, 985, 1446, 1459, 1573, 1707, 1752
MluCI AATT 8 cut(s) 96, 374, 386, 670, 873, 969, 1136, 1220
MlyI GAGTC 5 cut(s) 209, 493, 650, 856, 1221
MmeI TCCRAC 2 cut(s) 822, 906
MseI TTAA 6 cut(s) 62, 294, 596, 654, 792, 1404
MspA1I CMGCKG 1 cut(s) 1772
MspI CCGG 3 cut(s) 531, 1681, 1746
MspR9I CCNGG 2 cut(s) 710, 1747
MssI GTTTAAAC 1 cut(s) 1405
MvaI CCWGG 1 cut(s) 710
MvnI CGCG 2 cut(s) 1308, 1488
MwoI GCNNNNNNNGC 1 cut(s) 1233
NciI CCSGG 1 cut(s) 1747
NdeII GATC 9 cut(s) 101, 238, 312, 635, 787, 1087, 1559, 1644, 1677
NlaIII CATG 5 cut(s) 518, 962, 1034, 1124, 1373
NlaIV GGNNCC 3 cut(s) 637, 681, 1561
NmeAIII GCCGAG 1 cut(s) 1059
NmuCI GTSAC 2 cut(s) 163, 901
NspI RCATGY 3 cut(s) 962, 1034, 1373
PciI ACATGT 1 cut(s) 1369
PcsI WCGNNNNNNNCGW 2 cut(s) 1305, 1469
PfeI GAWTC 7 cut(s) 85, 206, 329, 461, 611, 647, 1498
PleI GAGTC 5 cut(s) 208, 493, 649, 855, 1220
PmeI GTTTAAAC 1 cut(s) 1405
PpsI GAGTC 5 cut(s) 208, 493, 649, 855, 1220
PscI ACATGT 1 cut(s) 1369
PshBI ATTAAT 1 cut(s) 62
Psp124BI GAGCTC 2 cut(s) 112, 1446
Psp6I CCWGG 1 cut(s) 708
PspGI CCWGG 1 cut(s) 708
PspN4I GGNNCC 3 cut(s) 637, 681, 1561
PspPI GGNCC 1 cut(s) 283
PstI CTGCAG 1 cut(s) 1164
PsuI RGATCY 3 cut(s) 101, 635, 1559
PvuII CAGCTG 1 cut(s) 1772
RsaI GTAC 1 cut(s) 1064
RsaNI GTAC 1 cut(s) 1063
SacI GAGCTC 2 cut(s) 112, 1446
SaqAI TTAA 6 cut(s) 62, 294, 596, 654, 792, 1404
Sau3AI GATC 9 cut(s) 101, 238, 312, 635, 787, 1087, 1559, 1644, 1677
Sau96I GGNCC 1 cut(s) 283
SbfI CCTGCAGG 1 cut(s) 1164
SchI GAGTC 5 cut(s) 209, 493, 650, 856, 1221
ScrFI CCNGG 2 cut(s) 710, 1747
SdaI CCTGCAGG 1 cut(s) 1164
SduI GDGCHC 7 cut(s) 112, 985, 1446, 1459, 1573, 1707, 1752
SfaNI GCATC 4 cut(s) 407, 496, 863, 1114
SfcI CTRYAG 2 cut(s) 1160, 1708
SinI GGWCC 1 cut(s) 283
SmlI CTYRAG 2 cut(s) 1091, 1145
SmoI CTYRAG 2 cut(s) 1091, 1145
SpeI ACTAGT 1 cut(s) 1579
Sse8387I CCTGCAGG 1 cut(s) 1164
Sse9I AATT 8 cut(s) 96, 374, 386, 670, 873, 969, 1136, 1220
SsiI CCGC 9 cut(s) 31, 808, 1271, 1306, 1336, 1430, 1486, 1511, 1716
SspMI CTAG 4 cut(s) 579, 753, 1452, 1580
SstI GAGCTC 2 cut(s) 112, 1446
StyD4I CCNGG 2 cut(s) 708, 1745
StyI CCWWGG 1 cut(s) 481
TaaI ACNGT 1 cut(s) 835
TaiI ACGT 1 cut(s) 1302
TaqI TCGA 4 cut(s) 477, 1215, 1395, 1598
TaqII GACCGA 1 cut(s) 393
TasI AATT 8 cut(s) 96, 374, 386, 670, 873, 969, 1136, 1220
TauI GCSGC 2 cut(s) 33, 1338
TfiI GAWTC 7 cut(s) 85, 206, 329, 461, 611, 647, 1498
Tru1I TTAA 6 cut(s) 62, 294, 596, 654, 792, 1404
Tru9I TTAA 6 cut(s) 62, 294, 596, 654, 792, 1404
TscAI CASTG 1 cut(s) 168
TseFI GTSAC 2 cut(s) 163, 901
TseI GCWGC 9 cut(s) 150, 606, 718, 820, 1294, 1615, 1684, 1769, 1772
Tsp45I GTSAC 2 cut(s) 163, 901
TspDTI ATGAA 4 cut(s) 4, 885, 969, 1341
TspRI CASTG 1 cut(s) 168
VneI GTGCAC 1 cut(s) 1569
VpaK11BI GGWCC 1 cut(s) 283
VspI ATTAAT 1 cut(s) 62
XapI RAATTY 2 cut(s) 386, 969
XceI RCATGY 3 cut(s) 962, 1034, 1373
XcmI CCANNNNNNNNNTGG 1 cut(s) 542
XspI CTAG 4 cut(s) 579, 753, 1452, 1580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.