MD07G1236000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004912
Physical Location & Seq
Reverse (-)
30906765 .. 30907249
485 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1236000.v1.1.491

Sequence Viewer

Length: 465 bp
ATGGCATTGCAAGATGATATAAGCCGCCTGAAGGTAGAAGTTGCTGATAAAAATGGACATGTGGAAGCTGTAAATGAAGACTTGGACATGTTTAAACTGAAATATGACATGCTGATGGCGGAGAAAAACGAGCTCAATGCTAGGGCTCAAACGCTCATGGCGAATGTGAGTTCCCGCGACAATCAGATTCAGGAAATGGCGGGACATCTTAGTCGGTTACAAACAGAACACGAGGGTCTGATTGTTGGATCCGAAAGTGCGCGTAGGCTAGTAGATGAGCTGAAAACAAGAGTGGAGGAGTTGCAGAAAGAGGTGAAAAGGCAGAGTGTTGTGATCTCGGATGGGGCTGAGGAGAAAAGAGAGGTGATCCGGCAGCTTTGTTTCTCGCTGGAGCACAACAGGAGCGGATACCAAGAGCTCCGCCAAGCGTTTACCGGGCACAGGCAGCGCCTAGTATGTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2 KIP1

Protein Analysis

155

Amino Acids

17.75

Weight (kDa)

5.78

Isoelectric Point (pI)

49.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 210
AccBSI CCGCTC 1 cut(s) 405
AccII CGCG 2 cut(s) 177, 262
AciI CCGC 6 cut(s) 25, 119, 175, 200, 405, 421
AclWI GGATC 3 cut(s) 243, 256, 361
AcuI CTGAAG 1 cut(s) 50
AfiI CCNNNNNNNGG 2 cut(s) 31, 441
AflIII ACRYGT 2 cut(s) 58, 87
AluBI AGCT 5 cut(s) 68, 133, 280, 376, 418
AluI AGCT 5 cut(s) 68, 133, 280, 376, 418
Alw21I GWGCWC 3 cut(s) 135, 396, 420
AlwI GGATC 3 cut(s) 243, 256, 361
ApeKI GCWGC 2 cut(s) 373, 445
AspLEI GCGC 2 cut(s) 262, 450
AsuC2I CCSGG 1 cut(s) 436
AsuHPI GGTGA 2 cut(s) 325, 376
BaeGI GKGCMC 1 cut(s) 441
BamHI GGATCC 1 cut(s) 248
BanII GRGCYC 3 cut(s) 135, 148, 420
BauI CACGAG 1 cut(s) 230
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 3 cut(s) 135, 396, 420
BbvCI CCTCAGC 1 cut(s) 348
BbvI GCAGC 2 cut(s) 385, 457
BccI CCATC 2 cut(s) 109, 335
BcgI CGANNNNNNTGC 2 cut(s) 119, 153
BciVI GTATCC 1 cut(s) 401
BcnI CCSGG 1 cut(s) 436
BfaI CTAG 3 cut(s) 141, 269, 452
BfoI RGCGCY 1 cut(s) 451
BfuI GTATCC 1 cut(s) 401
BisI GCNGC 3 cut(s) 25, 374, 446
BlsI GCNGC 3 cut(s) 26, 375, 447
Bme1390I CCNGG 1 cut(s) 436
BmiI GGNNCC 1 cut(s) 250
BmrFI CCNGG 1 cut(s) 436
BpiI GAAGAC 1 cut(s) 84
BpmI CTGGAG 1 cut(s) 410
Bpu10I CCTNAGC 1 cut(s) 348
BpuMI CCSGG 1 cut(s) 436
Bsc4I CCNNNNNNNGG 2 cut(s) 31, 441
Bse3DI GCAATG 1 cut(s) 5
BseGI GGATG 1 cut(s) 346
BseLI CCNNNNNNNGG 2 cut(s) 31, 441
BseMI GCAATG 1 cut(s) 5
BseMII CTCAG 1 cut(s) 339
BseRI GAGGAG 2 cut(s) 311, 365
BseSI GKGCMC 1 cut(s) 441
BseXI GCAGC 2 cut(s) 385, 457
Bsh1236I CGCG 2 cut(s) 177, 262
BsiHKAI GWGCWC 3 cut(s) 135, 396, 420
BsiSI CCGG 2 cut(s) 370, 435
BslFI GGGAC 1 cut(s) 216
BslI CCNNNNNNNGG 2 cut(s) 31, 441
BsmFI GGGAC 1 cut(s) 216
Bsp1286I GDGCHC 5 cut(s) 135, 148, 396, 420, 441
Bsp143I GATC 3 cut(s) 248, 333, 366
BspACI CCGC 6 cut(s) 25, 119, 175, 200, 405, 421
BspCNI CTCAG 1 cut(s) 340
BspFNI CGCG 2 cut(s) 177, 262
BspLI GGNNCC 1 cut(s) 250
BspPI GGATC 3 cut(s) 243, 256, 361
BsrBI CCGCTC 1 cut(s) 405
BsrDI GCAATG 1 cut(s) 5
BssMI GATC 3 cut(s) 248, 333, 366
BssSI CACGAG 1 cut(s) 230
Bst2BI CACGAG 1 cut(s) 230
BstDEI CTNAG 2 cut(s) 209, 348
BstF5I GGATG 1 cut(s) 346
BstFNI CGCG 2 cut(s) 177, 262
BstH2I RGCGCY 1 cut(s) 451
BstHHI GCGC 2 cut(s) 262, 450
BstKTI GATC 3 cut(s) 251, 336, 369
BstMBI GATC 3 cut(s) 248, 333, 366
BstMWI GCNNNNNNNGC 1 cut(s) 445
BstNSI RCATGY 3 cut(s) 62, 91, 112
BstSCI CCNGG 1 cut(s) 434
BstSLI GKGCMC 1 cut(s) 441
BstUI CGCG 2 cut(s) 177, 262
BstV1I GCAGC 2 cut(s) 385, 457
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 1 cut(s) 248
BstYI RGATCY 1 cut(s) 248
BsuI GTATCC 1 cut(s) 401
BtsCI GGATG 1 cut(s) 346
CfoI GCGC 2 cut(s) 262, 450
CviAII CATG 4 cut(s) 59, 88, 109, 157
CviJI RGCY 9 cut(s) 24, 68, 133, 146, 268, 280, 347, 376, 418
CviKI_1 RGCY 9 cut(s) 24, 68, 133, 146, 268, 280, 347, 376, 418
DdeI CTNAG 2 cut(s) 209, 348
DpnI GATC 3 cut(s) 250, 335, 368
DpnII GATC 3 cut(s) 248, 333, 366
DraI TTTAAA 1 cut(s) 94
DrdI GACNNNNNNGTC 1 cut(s) 210
DseDI GACNNNNNNGTC 1 cut(s) 210
EciI GGCGGA 2 cut(s) 134, 410
Ecl136II GAGCTC 2 cut(s) 133, 418
Eco24I GRGCYC 3 cut(s) 135, 148, 420
Eco53kI GAGCTC 2 cut(s) 133, 418
Eco57I CTGAAG 1 cut(s) 50
EcoICRI GAGCTC 2 cut(s) 133, 418
EcoT38I GRGCYC 3 cut(s) 135, 148, 420
FaeI CATG 4 cut(s) 62, 91, 112, 160
FaiI YATR 7 cut(s) 20, 60, 89, 105, 110, 158, 457
FaqI GGGAC 1 cut(s) 216
FatI CATG 4 cut(s) 58, 87, 108, 156
FauI CCCGC 2 cut(s) 182, 193
Fnu4HI GCNGC 3 cut(s) 25, 374, 446
FokI GGATG 1 cut(s) 353
FriOI GRGCYC 3 cut(s) 135, 148, 420
Fsp4HI GCNGC 3 cut(s) 25, 374, 446
FspBI CTAG 3 cut(s) 141, 269, 452
GlaI GCGC 2 cut(s) 261, 449
GluI GCNGC 3 cut(s) 25, 374, 446
GsuI CTGGAG 1 cut(s) 410
HaeII RGCGCY 1 cut(s) 451
HapII CCGG 2 cut(s) 370, 435
HhaI GCGC 2 cut(s) 262, 450
Hin1II CATG 4 cut(s) 62, 91, 112, 160
Hin6I GCGC 2 cut(s) 260, 448
HinP1I GCGC 2 cut(s) 260, 448
HinfI GANTC 1 cut(s) 187
HpaII CCGG 2 cut(s) 370, 435
HphI GGTGA 2 cut(s) 325, 376
Hpy166II GTNNAC 1 cut(s) 432
Hpy188I TCNGA 5 cut(s) 186, 240, 253, 340, 464
Hpy188III TCNNGA 1 cut(s) 191
Hpy8I GTNNAC 1 cut(s) 432
HpyAV CCTTC 1 cut(s) 25
HpyCH4V TGCA 2 cut(s) 10, 304
HpyF10VI GCNNNNNNNGC 1 cut(s) 445
HpyF3I CTNAG 2 cut(s) 209, 348
Hsp92II CATG 4 cut(s) 62, 91, 112, 160
HspAI GCGC 2 cut(s) 260, 448
Kzo9I GATC 3 cut(s) 248, 333, 366
LmnI GCTCC 3 cut(s) 391, 402, 423
LpnPI CCDG 7 cut(s) 41, 176, 374, 383, 385, 427, 448
Lsp1109I GCAGC 2 cut(s) 385, 457
MaeI CTAG 3 cut(s) 141, 269, 452
MaeIII GTNAC 1 cut(s) 216
MalI GATC 3 cut(s) 250, 335, 368
MbiI CCGCTC 1 cut(s) 405
MboI GATC 3 cut(s) 248, 333, 366
MboII GAAGA 1 cut(s) 89
MflI RGATCY 1 cut(s) 248
MhlI GDGCHC 5 cut(s) 135, 148, 396, 420, 441
MmeI TCCRAC 1 cut(s) 226
MnlI CCTC 5 cut(s) 226, 289, 304, 343, 355
MseI TTAA 1 cut(s) 93
MslI CAYNNNNRTG 1 cut(s) 113
MspI CCGG 2 cut(s) 370, 435
MspR9I CCNGG 1 cut(s) 436
MssI GTTTAAAC 1 cut(s) 94
MvnI CGCG 2 cut(s) 177, 262
MwoI GCNNNNNNNGC 1 cut(s) 445
NciI CCSGG 1 cut(s) 436
NdeII GATC 3 cut(s) 248, 333, 366
NlaIII CATG 4 cut(s) 62, 91, 112, 160
NlaIV GGNNCC 1 cut(s) 250
NspI RCATGY 3 cut(s) 62, 91, 112
PciI ACATGT 2 cut(s) 58, 87
PcsI WCGNNNNNNNCGW 1 cut(s) 158
PfeI GAWTC 1 cut(s) 187
PkrI GCNGC 3 cut(s) 26, 375, 447
PmeI GTTTAAAC 1 cut(s) 94
PscI ACATGT 2 cut(s) 58, 87
Psp124BI GAGCTC 2 cut(s) 135, 420
PspN4I GGNNCC 1 cut(s) 250
PsuI RGATCY 1 cut(s) 248
RseI CAYNNNNRTG 1 cut(s) 113
SacI GAGCTC 2 cut(s) 135, 420
SaqAI TTAA 1 cut(s) 93
SatI GCNGC 3 cut(s) 25, 374, 446
Sau3AI GATC 3 cut(s) 248, 333, 366
ScrFI CCNGG 1 cut(s) 436
SduI GDGCHC 5 cut(s) 135, 148, 396, 420, 441
SetI ASST 8 cut(s) 36, 70, 135, 282, 315, 366, 378, 420
SmiMI CAYNNNNRTG 1 cut(s) 113
SsiI CCGC 6 cut(s) 25, 119, 175, 200, 405, 421
SspMI CTAG 3 cut(s) 141, 269, 452
SstI GAGCTC 2 cut(s) 135, 420
StyD4I CCNGG 1 cut(s) 434
TauI GCSGC 1 cut(s) 27
TfiI GAWTC 1 cut(s) 187
Tru1I TTAA 1 cut(s) 93
Tru9I TTAA 1 cut(s) 93
TseI GCWGC 2 cut(s) 373, 445
TspDTI ATGAA 1 cut(s) 90
XceI RCATGY 3 cut(s) 62, 91, 112
XspI CTAG 3 cut(s) 141, 269, 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.