MD07G1236100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004912
Physical Location & Seq
Reverse (-)
30907251 .. 30908930
1680 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1236100.v1.1.491

Sequence Viewer

Length: 459 bp
ATGTGGCTGGTAGAATATCTGGAAGAGATGGACCAGAGCATTAAGCAAATGCTGAAGCTGATCAAAGAAGATGGTGATTCTTTGCCCAACAAAGACGAGATGTTTTTGCAGAAGATGTTGGAATTAGTTCAGGAATTTCACCGGATGTATCGGTCACTAGCCGGGTGCTATGAACACTTGACCAAAGAAGCACATAAGGGGTTTGGAGGTTCTGAATATGGTTTCAACCAATGCTCTCCTCTGCTGACTCCCGATGCTAAACATGGTTTGCGGAAATCCGACGATCAAGTTGTTGGTTTTGATATATCACTAAGCTCAGATGGTTCTAGTCCAGCTCTTACTTTAAAGAATGGCGCTGAATCGTCTTCCTCTTCATCATTGGGATCCGAGTCAGATTCTCCTAATTCCCTGGTCAGCAATTACTTGGTTCCACCTCCGAGCACTGATTTCGACAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2 KIP1

Protein Analysis

153

Amino Acids

16.8

Weight (kDa)

4.64

Isoelectric Point (pI)

57.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 2 - 57 1.7e-15 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 271
AclWI GGATC 2 cut(s) 378, 391
AcsI RAATTY 1 cut(s) 134
AcuI CTGAAG 1 cut(s) 74
AgsI TTSAA 1 cut(s) 226
AjnI CCWGG 1 cut(s) 408
AluBI AGCT 3 cut(s) 58, 315, 335
AluI AGCT 3 cut(s) 58, 315, 335
Alw21I GWGCWC 1 cut(s) 443
AlwI GGATC 2 cut(s) 378, 391
ApoI RAATTY 1 cut(s) 134
Asp700I GAANNNNTTC 1 cut(s) 126
AspLEI GCGC 1 cut(s) 356
AspS9I GGNCC 1 cut(s) 31
AsuC2I CCSGG 1 cut(s) 163
AsuHPI GGTGA 2 cut(s) 86, 131
AvaII GGWCC 1 cut(s) 31
BamHI GGATCC 1 cut(s) 383
BbsI GAAGAC 1 cut(s) 357
Bbv12I GWGCWC 1 cut(s) 443
BccI CCATC 3 cut(s) 22, 65, 314
BcgI CGANNNNNNTGC 1 cut(s) 430
BciT130I CCWGG 1 cut(s) 410
BclI TGATCA 1 cut(s) 60
BcnI CCSGG 1 cut(s) 163
BfaI CTAG 2 cut(s) 158, 327
BfoI RGCGCY 1 cut(s) 357
Bme1390I CCNGG 2 cut(s) 163, 410
Bme18I GGWCC 1 cut(s) 31
BmgT120I GGNCC 1 cut(s) 31
BmiI GGNNCC 2 cut(s) 385, 429
BmrFI CCNGG 2 cut(s) 163, 410
BmsI GCATC 1 cut(s) 244
BpiI GAAGAC 1 cut(s) 357
BpuMI CCSGG 1 cut(s) 163
BsaJI CCNNGG 1 cut(s) 408
BsaWI WCCGGW 1 cut(s) 141
BseBI CCWGG 1 cut(s) 410
BseDI CCNNGG 1 cut(s) 408
BseGI GGATG 1 cut(s) 150
BseMII CTCAG 1 cut(s) 330
BseRI GAGGAG 1 cut(s) 228
BsiHKAI GWGCWC 1 cut(s) 443
BsiSI CCGG 2 cut(s) 142, 162
Bsp1286I GDGCHC 1 cut(s) 443
Bsp143I GATC 3 cut(s) 60, 283, 383
BspACI CCGC 1 cut(s) 271
BspCNI CTCAG 1 cut(s) 329
BspLI GGNNCC 2 cut(s) 385, 429
BspPI GGATC 2 cut(s) 378, 391
BssECI CCNNGG 1 cut(s) 408
BssMI GATC 3 cut(s) 60, 283, 383
Bst2UI CCWGG 1 cut(s) 410
Bst4CI ACNGT 1 cut(s) 455
Bst6I CTCTTC 2 cut(s) 18, 376
BstDEI CTNAG 2 cut(s) 311, 316
BstF5I GGATG 1 cut(s) 150
BstH2I RGCGCY 1 cut(s) 357
BstHHI GCGC 1 cut(s) 356
BstKTI GATC 3 cut(s) 63, 286, 386
BstMBI GATC 3 cut(s) 60, 283, 383
BstNI CCWGG 1 cut(s) 410
BstSCI CCNGG 2 cut(s) 161, 408
BstV2I GAAGAC 1 cut(s) 357
BstX2I RGATCY 1 cut(s) 383
BstYI RGATCY 1 cut(s) 383
BtsCI GGATG 1 cut(s) 150
BtsIMutI CAGTG 1 cut(s) 441
CfoI GCGC 1 cut(s) 356
Cfr13I GGNCC 1 cut(s) 31
CviAII CATG 1 cut(s) 263
CviJI RGCY 5 cut(s) 7, 58, 161, 315, 335
CviKI_1 RGCY 5 cut(s) 7, 58, 161, 315, 335
DdeI CTNAG 2 cut(s) 311, 316
DpnI GATC 3 cut(s) 62, 285, 385
DpnII GATC 3 cut(s) 60, 283, 383
DraI TTTAAA 1 cut(s) 345
Eam1104I CTCTTC 2 cut(s) 18, 376
EarI CTCTTC 2 cut(s) 18, 376
Eco47I GGWCC 1 cut(s) 31
Eco57I CTGAAG 1 cut(s) 74
EcoRII CCWGG 1 cut(s) 408
FaeI CATG 1 cut(s) 266
FaiI YATR 5 cut(s) 171, 195, 219, 264, 305
FatI CATG 1 cut(s) 262
FbaI TGATCA 1 cut(s) 60
FokI GGATG 1 cut(s) 157
FspBI CTAG 2 cut(s) 158, 327
GlaI GCGC 1 cut(s) 355
HaeII RGCGCY 1 cut(s) 357
HapII CCGG 2 cut(s) 142, 162
HhaI GCGC 1 cut(s) 356
Hin1II CATG 1 cut(s) 266
Hin6I GCGC 1 cut(s) 354
HinP1I GCGC 1 cut(s) 354
HinfI GANTC 5 cut(s) 77, 247, 359, 389, 395
HpaII CCGG 2 cut(s) 142, 162
HphI GGTGA 2 cut(s) 86, 131
Hpy188I TCNGA 6 cut(s) 214, 280, 319, 388, 394, 438
Hpy188III TCNNGA 3 cut(s) 20, 131, 251
Hpy99I CGWCG 1 cut(s) 284
HpyCH4III ACNGT 1 cut(s) 455
HpyCH4V TGCA 1 cut(s) 109
HpyF3I CTNAG 2 cut(s) 311, 316
Hsp92II CATG 1 cut(s) 266
HspAI GCGC 1 cut(s) 354
Ksp22I TGATCA 1 cut(s) 60
Kzo9I GATC 3 cut(s) 60, 283, 383
LpnPI CCDG 8 cut(s) 5, 47, 116, 155, 175, 345, 395, 422
LweI GCATC 1 cut(s) 244
MaeI CTAG 2 cut(s) 158, 327
MaeIII GTNAC 1 cut(s) 153
MalI GATC 3 cut(s) 62, 285, 385
MboI GATC 3 cut(s) 60, 283, 383
MboII GAAGA 5 cut(s) 35, 80, 124, 357, 363
MflI RGATCY 1 cut(s) 383
MhlI GDGCHC 1 cut(s) 443
MluCI AATT 4 cut(s) 122, 134, 403, 418
MlyI GAGTC 2 cut(s) 241, 398
MmeI TCCRAC 2 cut(s) 99, 303
MnlI CCTC 4 cut(s) 200, 249, 379, 444
MroXI GAANNNNTTC 1 cut(s) 126
MseI TTAA 3 cut(s) 42, 344, 457
MspI CCGG 2 cut(s) 142, 162
MspR9I CCNGG 2 cut(s) 163, 410
MvaI CCWGG 1 cut(s) 410
NciI CCSGG 1 cut(s) 163
NdeII GATC 3 cut(s) 60, 283, 383
NlaIII CATG 1 cut(s) 266
NlaIV GGNNCC 2 cut(s) 385, 429
NmuCI GTSAC 1 cut(s) 153
PdmI GAANNNNTTC 1 cut(s) 126
PfeI GAWTC 3 cut(s) 77, 359, 395
PleI GAGTC 2 cut(s) 241, 397
PpsI GAGTC 2 cut(s) 241, 397
Psp6I CCWGG 1 cut(s) 408
PspGI CCWGG 1 cut(s) 408
PspN4I GGNNCC 2 cut(s) 385, 429
PspPI GGNCC 1 cut(s) 31
PsuI RGATCY 1 cut(s) 383
SaqAI TTAA 3 cut(s) 42, 344, 457
Sau3AI GATC 3 cut(s) 60, 283, 383
Sau96I GGNCC 1 cut(s) 31
SchI GAGTC 2 cut(s) 241, 398
ScrFI CCNGG 2 cut(s) 163, 410
SduI GDGCHC 1 cut(s) 443
SetI ASST 5 cut(s) 60, 211, 317, 337, 436
SfaNI GCATC 1 cut(s) 244
SinI GGWCC 1 cut(s) 31
Sse9I AATT 4 cut(s) 122, 134, 403, 418
SsiI CCGC 1 cut(s) 271
SspMI CTAG 2 cut(s) 158, 327
StyD4I CCNGG 2 cut(s) 161, 408
TaaI ACNGT 1 cut(s) 455
TaqI TCGA 1 cut(s) 450
TaqII GACCGA 1 cut(s) 141
TasI AATT 4 cut(s) 122, 134, 403, 418
TfiI GAWTC 3 cut(s) 77, 359, 395
Tru1I TTAA 3 cut(s) 42, 344, 457
Tru9I TTAA 3 cut(s) 42, 344, 457
TscAI CASTG 1 cut(s) 448
TseFI GTSAC 1 cut(s) 153
Tsp45I GTSAC 1 cut(s) 153
TspDTI ATGAA 2 cut(s) 186, 363
TspRI CASTG 1 cut(s) 448
VpaK11BI GGWCC 1 cut(s) 31
XapI RAATTY 1 cut(s) 134
XmnI GAANNNNTTC 1 cut(s) 126
XspI CTAG 2 cut(s) 158, 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.