MD01G1036900.v1.1

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012774
Physical Location & Seq
Forward (+)
12378071 .. 12379372
1302 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1036900.v1.1.491

Sequence Viewer

Length: 1302 bp
ATGGAGTTTGATCACAGAAAAGTCCTAGCACAAACAAATTTGAGTGAGCAATTAGTTTGTTCAATTCCTTGTAATCCACATCTGAACAAAGGGGGTTATTGCTTTCAATCTTGTTTCAAAACCTGCCCTGATGTCTGTAAGAAACTTATAGTCTATGTTGGTGGTGAGCTTGCTCCTCCAATTGTACCATACCCTCCTCCGCCGCCATCTCCTCGGCTGCCACCGCTAGCTGAATATTATTATCAGCCTGAATCTGAGCGGAAGCGTCTGATTGAAACGAGTATGACTATCACCGCTTGTATGATTGGGGCTGCTCTTCTTCTCGCTATTGTTTTCTCACTTGTTAGATTTTATCGCCGCCGCCGCGATGATTCGACGAGGAGGAGTGGTTCTTTCCCGATTTTGTTTGATACCCAACAAGATTTTCTTGATGAAGATCATGGTCCTGTTCTTGATCATCCCATTTGGTACATCCACACTGTTGGTCTCCCACAATCGGCGATTGACTCCATAACCGTCTGCAAGTTTCGAAAAGATGAGGGTTTGATTGAGGGAACTGATTGTTCTGTTTGCTTGAGTGAGTTTGAAGAAGATGAGAGTCTTAGACTTTTGCCCAAATGCAGTCATGCCTTTCACATCCCTTGTATTGATACCTGGTTGAGATCACATAAGAATTGCCCGCTTTGCCGTGCTCCGATTGTTTGTGACACTGCTACTGTTCAGGTGAGTGTCCCAGAGTCTGTTTCCAGTGGTTCGGGTGTGAGGGATAGCATTCAGGTGGAGAATTTGGAGGGTGATGAAGGGGTTGGAAGTGTTGGAAGAGGGACTAGTGAGGTTGGGGTTGGAGATGATGGGAATGTTTGTGAAACTCGAATTGAGGGAAGAAGCACGGAAAATTCGAGGAAGGTTTTGCCAAATTCCATTGTTCCTGTGGGAATTTGTGATCCAAGAGCATTCAGTGATTTGTCTGACAATCGACGGGTTACTGAAGAGGATGTTCAACCAATGAGGAGATCAGGTTCGATGGATTCATTATCTGCTTTGAGAATATATCGTGATGTGGCGAATGTAATTCCCGAAGAAGGAAGTTCGAATTCCCAATCACATGTAAAGAATATGAATTCGGGGATTGTTTCAAAGCATGGCAGTTCAAGTTCCAGCCTGTCCAAACTAATGAGAAGCTCTTCAATTGGGTTTTCTCTGCAGATGGGGCCAATATCAATGAAAAGGCCATTCGCATCTGGCAGGAAGCTTTTTTCATCCAAACATGGAAGGAGTCATAGCTCAATCCTACCCTTATGA

Protein Analysis

434

Amino Acids

47.66

Weight (kDa)

6.47

Isoelectric Point (pI)

61.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 187 - 230 3.2e-14 Ring finger domain
zf-RING_11 PF17123 187 - 216 1.2e-10 RING-like zinc finger
zf-C3HC4 PF00097 188 - 229 1.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 188 - 229 1e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 190 - 230 5.4e-09 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012774)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10380
fragaria_vesca FvH4_1g20164
malus_domestica MD01G1036900.v1.1 MD15G1317400.v1.1
prunus_persica Prupe.6G194300_v2.0.a1
pyrus_communis pycom01g06390 pycom15g27980
rosa_chinensis RchiOBHm_Chr2g0112241
rosa_laevigata RLG00000017962
rosa_multiflora Rmu_co8477273.1_g000001 Rmu_sc0001386.1_g000026
rosa_roxburghii Rroxscaffold_2G00131620
rosa_rugosa Rorug02G0179300
rosa_samantha Rh2AG231700 Rh2BG245200 Rh2CG236100 Rh2DG239600
rosa_wichuraiana Rw2G017980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 131
AccBSI CCGCTC 1 cut(s) 259
AccII CGCG 1 cut(s) 366
AciI CCGC 9 cut(s) 200, 203, 224, 259, 294, 358, 361, 364, 680
AclWI GGATC 1 cut(s) 938
AcsI RAATTY 7 cut(s) 37, 784, 895, 916, 936, 1093, 1120
AcuI CTGAAG 1 cut(s) 1008
AfaI GTAC 2 cut(s) 186, 470
AfiI CCNNNNNNNGG 2 cut(s) 496, 1082
AflIII ACRYGT 1 cut(s) 1105
AgsI TTSAA 9 cut(s) 63, 107, 118, 275, 587, 1001, 1137, 1152, 1188
AhlI ACTAGT 1 cut(s) 827
AjnI CCWGG 1 cut(s) 653
AjuI GAANNNNNNNTTGG 2 cut(s) 408, 440
AloI GAACNNNNNNTCC 4 cut(s) 373, 405, 1003, 1035
AluBI AGCT 5 cut(s) 169, 230, 1182, 1252, 1284
AluI AGCT 5 cut(s) 169, 230, 1182, 1252, 1284
Alw21I GWGCWC 1 cut(s) 694
Alw26I GTCTC 1 cut(s) 491
AlwI GGATC 1 cut(s) 938
AlwNI CAGNNNCTG 1 cut(s) 740
AoxI GGCC 2 cut(s) 1211, 1229
ApeKI GCWGC 2 cut(s) 217, 311
ApoI RAATTY 7 cut(s) 37, 784, 895, 916, 936, 1093, 1120
Asp700I GAANNNNTTC 1 cut(s) 1183
AspS9I GGNCC 2 cut(s) 443, 1211
AsuHPI GGTGA 4 cut(s) 176, 283, 736, 806
AsuII TTCGAA 2 cut(s) 529, 1091
AsuNHI GCTAGC 1 cut(s) 226
AvaII GGWCC 1 cut(s) 443
Bbv12I GWGCWC 1 cut(s) 694
BbvI GCAGC 2 cut(s) 204, 298
BccI CCATC 4 cut(s) 214, 845, 1018, 1201
BceAI ACGGC 1 cut(s) 672
BciT130I CCWGG 1 cut(s) 655
BclI TGATCA 2 cut(s) 10, 454
BcoDI GTCTC 1 cut(s) 491
BcuI ACTAGT 1 cut(s) 827
BfaI CTAG 3 cut(s) 26, 227, 828
BfmI CTRYAG 1 cut(s) 1202
BfuAI ACCTGC 1 cut(s) 131
BisI GCNGC 6 cut(s) 203, 218, 312, 358, 361, 364
BlsI GCNGC 6 cut(s) 204, 219, 313, 359, 362, 365
Bme1390I CCNGG 1 cut(s) 655
Bme18I GGWCC 1 cut(s) 443
BmgT120I GGNCC 2 cut(s) 443, 1211
BmiI GGNNCC 1 cut(s) 1212
BmrFI CCNGG 1 cut(s) 655
BmsI GCATC 1 cut(s) 1247
BmtI GCTAGC 1 cut(s) 230
Bpu14I TTCGAA 2 cut(s) 529, 1091
BpuEI CTTGAG 1 cut(s) 595
BsaBI GATNNNNATC 1 cut(s) 435
BsaI GGTCTC 1 cut(s) 491
BsaJI CCNNGG 1 cut(s) 212
BsaXI ACNNNNNCTCC 4 cut(s) 373, 403, 1003, 1033
Bsc4I CCNNNNNNNGG 2 cut(s) 496, 1082
Bse1I ACTGG 1 cut(s) 747
Bse8I GATNNNNATC 1 cut(s) 435
BseBI CCWGG 1 cut(s) 655
BseDI CCNNGG 1 cut(s) 212
BseGI GGATG 5 cut(s) 457, 471, 636, 1000, 1259
BseJI GATNNNNATC 1 cut(s) 435
BseLI CCNNNNNNNGG 2 cut(s) 496, 1082
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 1 cut(s) 747
BseRI GAGGAG 6 cut(s) 165, 186, 201, 394, 397, 1024
BseXI GCAGC 2 cut(s) 204, 298
Bsh1236I CGCG 1 cut(s) 366
BshFI GGCC 2 cut(s) 1213, 1231
BsiHKAI GWGCWC 1 cut(s) 694
BslFI GGGAC 2 cut(s) 716, 838
BslI CCNNNNNNNGG 2 cut(s) 496, 1082
BsmAI GTCTC 1 cut(s) 491
BsmFI GGGAC 2 cut(s) 716, 838
BsmI GAATGC 2 cut(s) 771, 953
BsnI GGCC 2 cut(s) 1213, 1231
Bso31I GGTCTC 1 cut(s) 491
Bsp119I TTCGAA 2 cut(s) 529, 1091
Bsp1286I GDGCHC 1 cut(s) 694
Bsp143I GATC 6 cut(s) 10, 436, 454, 662, 943, 1013
BspACI CCGC 9 cut(s) 200, 203, 224, 259, 294, 358, 361, 364, 680
BspANI GGCC 2 cut(s) 1213, 1231
BspCNI CTCAG 1 cut(s) 247
BspFNI CGCG 1 cut(s) 366
BspLI GGNNCC 1 cut(s) 1212
BspMAI CTGCAG 1 cut(s) 1206
BspMI ACCTGC 1 cut(s) 131
BspOI GCTAGC 1 cut(s) 230
BspPI GGATC 1 cut(s) 938
BspQI GCTCTTC 2 cut(s) 321, 1189
BspT104I TTCGAA 2 cut(s) 529, 1091
BspTNI GGTCTC 1 cut(s) 491
BsrBI CCGCTC 1 cut(s) 259
BsrI ACTGG 1 cut(s) 747
BssECI CCNNGG 1 cut(s) 212
BssMI GATC 6 cut(s) 10, 436, 454, 662, 943, 1013
Bst2UI CCWGG 1 cut(s) 655
Bst4CI ACNGT 3 cut(s) 481, 517, 718
Bst6I CTCTTC 4 cut(s) 321, 814, 984, 1189
BstBI TTCGAA 2 cut(s) 529, 1091
BstC8I GCNNGC 3 cut(s) 171, 228, 680
BstDEI CTNAG 2 cut(s) 255, 602
BstF5I GGATG 5 cut(s) 457, 471, 636, 1000, 1259
BstFNI CGCG 1 cut(s) 366
BstKTI GATC 6 cut(s) 13, 439, 457, 665, 946, 1016
BstMAI GTCTC 1 cut(s) 491
BstMBI GATC 6 cut(s) 10, 436, 454, 662, 943, 1013
BstMWI GCNNNNNNNGC 4 cut(s) 223, 363, 684, 1210
BstNI CCWGG 1 cut(s) 655
BstNSI RCATGY 1 cut(s) 1109
BstSCI CCNGG 1 cut(s) 653
BstSFI CTRYAG 1 cut(s) 1202
BstUI CGCG 1 cut(s) 366
BstV1I GCAGC 2 cut(s) 204, 298
BstXI CCANNNNNNTGG 1 cut(s) 482
BsuRI GGCC 2 cut(s) 1213, 1231
BtgZI GCGATG 1 cut(s) 381
BtsCI GGATG 5 cut(s) 457, 471, 636, 1000, 1259
BtsI GCAGTG 1 cut(s) 708
BtsIMutI CAGTG 4 cut(s) 477, 708, 754, 964
BveI ACCTGC 1 cut(s) 131
Cac8I GCNNGC 3 cut(s) 171, 228, 680
CaiI CAGNNNCTG 1 cut(s) 740
Cfr13I GGNCC 2 cut(s) 443, 1211
CseI GACGC 1 cut(s) 254
CsiI ACCWGGT 1 cut(s) 653
Csp6I GTAC 2 cut(s) 185, 469
CviAII CATG 5 cut(s) 440, 626, 1106, 1142, 1268
CviQI GTAC 2 cut(s) 185, 469
DdeI CTNAG 2 cut(s) 255, 602
DpnI GATC 6 cut(s) 12, 438, 456, 664, 945, 1015
DpnII GATC 6 cut(s) 10, 436, 454, 662, 943, 1013
Eam1104I CTCTTC 4 cut(s) 321, 814, 984, 1189
EarI CTCTTC 4 cut(s) 321, 814, 984, 1189
EciI GGCGGA 1 cut(s) 189
Eco31I GGTCTC 1 cut(s) 491
Eco47I GGWCC 1 cut(s) 443
Eco57I CTGAAG 1 cut(s) 1008
EcoRI GAATTC 2 cut(s) 1093, 1120
EcoRII CCWGG 1 cut(s) 653
FaeI CATG 5 cut(s) 443, 629, 1109, 1145, 1271
FalI AAGNNNNNCTT 2 cut(s) 411, 443
FaqI GGGAC 2 cut(s) 716, 838
FatI CATG 5 cut(s) 439, 625, 1105, 1141, 1267
FauI CCCGC 1 cut(s) 687
FbaI TGATCA 2 cut(s) 10, 454
Fnu4HI GCNGC 6 cut(s) 203, 218, 312, 358, 361, 364
FokI GGATG 5 cut(s) 444, 458, 623, 1007, 1246
Fsp4HI GCNGC 6 cut(s) 203, 218, 312, 358, 361, 364
FspBI CTAG 3 cut(s) 26, 227, 828
GluI GCNGC 6 cut(s) 203, 218, 312, 358, 361, 364
HaeIII GGCC 2 cut(s) 1213, 1231
HgaI GACGC 1 cut(s) 254
Hin1II CATG 5 cut(s) 443, 629, 1109, 1145, 1271
HindIII AAGCTT 1 cut(s) 1250
HinfI GANTC 7 cut(s) 251, 371, 506, 598, 737, 1028, 1276
HphI GGTGA 4 cut(s) 176, 283, 736, 806
Hpy188I TCNGA 5 cut(s) 84, 256, 270, 696, 970
Hpy188III TCNNGA 5 cut(s) 397, 428, 452, 1055, 1076
Hpy99I CGWCG 2 cut(s) 379, 981
HpyAV CCTTC 4 cut(s) 794, 898, 1076, 1266
HpyCH4III ACNGT 3 cut(s) 481, 517, 718
HpyCH4V TGCA 3 cut(s) 522, 621, 1204
HpyF10VI GCNNNNNNNGC 4 cut(s) 223, 363, 684, 1210
HpyF3I CTNAG 2 cut(s) 255, 602
Hsp92II CATG 5 cut(s) 443, 629, 1109, 1145, 1271
Ksp22I TGATCA 2 cut(s) 10, 454
Kzo9I GATC 6 cut(s) 10, 436, 454, 662, 943, 1013
LguI GCTCTTC 2 cut(s) 321, 1189
LmnI GCTCC 2 cut(s) 178, 697
Lsp1109I GCAGC 2 cut(s) 204, 298
LweI GCATC 1 cut(s) 1247
MabI ACCWGGT 1 cut(s) 653
MaeI CTAG 3 cut(s) 26, 227, 828
MaeIII GTNAC 2 cut(s) 704, 982
MalI GATC 6 cut(s) 12, 438, 456, 664, 945, 1015
MbiI CCGCTC 1 cut(s) 259
MboI GATC 6 cut(s) 10, 436, 454, 662, 943, 1013
MfeI CAATTG 2 cut(s) 180, 1188
MhlI GDGCHC 1 cut(s) 694
MlyI GAGTC 4 cut(s) 500, 607, 746, 1285
MmeI TCCRAC 3 cut(s) 787, 796, 823
MroXI GAANNNNTTC 1 cut(s) 1183
MslI CAYNNNNRTG 1 cut(s) 776
MspR9I CCNGG 1 cut(s) 655
MunI CAATTG 2 cut(s) 180, 1188
Mva1269I GAATGC 2 cut(s) 771, 953
MvaI CCWGG 1 cut(s) 655
MvnI CGCG 1 cut(s) 366
MwoI GCNNNNNNNGC 4 cut(s) 223, 363, 684, 1210
NdeII GATC 6 cut(s) 10, 436, 454, 662, 943, 1013
NheI GCTAGC 1 cut(s) 226
NlaIII CATG 5 cut(s) 443, 629, 1109, 1145, 1271
NlaIV GGNNCC 1 cut(s) 1212
NmeAIII GCCGAG 1 cut(s) 193
NmuCI GTSAC 1 cut(s) 704
NspI RCATGY 1 cut(s) 1109
NspV TTCGAA 2 cut(s) 529, 1091
PciI ACATGT 1 cut(s) 1105
PciSI GCTCTTC 2 cut(s) 321, 1189
PcsI WCGNNNNNNNCGW 1 cut(s) 896
PctI GAATGC 2 cut(s) 771, 953
PdmI GAANNNNTTC 1 cut(s) 1183
PfeI GAWTC 3 cut(s) 251, 371, 1028
PkrI GCNGC 6 cut(s) 204, 219, 313, 359, 362, 365
PleI GAGTC 4 cut(s) 500, 606, 745, 1284
PpsI GAGTC 4 cut(s) 500, 606, 745, 1284
PscI ACATGT 1 cut(s) 1105
Psp6I CCWGG 1 cut(s) 653
PspGI CCWGG 1 cut(s) 653
PspN4I GGNNCC 1 cut(s) 1212
PspPI GGNCC 2 cut(s) 443, 1211
PstI CTGCAG 1 cut(s) 1206
PstNI CAGNNNCTG 1 cut(s) 740
RsaI GTAC 2 cut(s) 186, 470
RsaNI GTAC 2 cut(s) 185, 469
RseI CAYNNNNRTG 1 cut(s) 776
SapI GCTCTTC 2 cut(s) 321, 1189
SatI GCNGC 6 cut(s) 203, 218, 312, 358, 361, 364
Sau3AI GATC 6 cut(s) 10, 436, 454, 662, 943, 1013
Sau96I GGNCC 2 cut(s) 443, 1211
SchI GAGTC 4 cut(s) 500, 607, 746, 1285
ScrFI CCNGG 1 cut(s) 655
SduI GDGCHC 1 cut(s) 694
SexAI ACCWGGT 1 cut(s) 653
SfaNI GCATC 1 cut(s) 1247
SfcI CTRYAG 1 cut(s) 1202
SfuI TTCGAA 2 cut(s) 529, 1091
SinI GGWCC 1 cut(s) 443
SmiMI CAYNNNNRTG 1 cut(s) 776
SmlI CTYRAG 1 cut(s) 574
SmoI CTYRAG 1 cut(s) 574
SpeI ACTAGT 1 cut(s) 827
SsiI CCGC 9 cut(s) 200, 203, 224, 259, 294, 358, 361, 364, 680
SspI AATATT 1 cut(s) 236
SspMI CTAG 3 cut(s) 26, 227, 828
StyD4I CCNGG 1 cut(s) 653
TaaI ACNGT 3 cut(s) 481, 517, 718
TaqI TCGA 7 cut(s) 374, 529, 871, 899, 976, 1022, 1091
TauI GCSGC 4 cut(s) 205, 360, 363, 366
TfiI GAWTC 3 cut(s) 251, 371, 1028
TscAI CASTG 4 cut(s) 484, 715, 754, 964
TseFI GTSAC 1 cut(s) 704
TseI GCWGC 2 cut(s) 217, 311
Tsp45I GTSAC 1 cut(s) 704
TspDTI ATGAA 6 cut(s) 447, 813, 1020, 1133, 1238, 1248
TspGWI ACGGA 1 cut(s) 905
TspRI CASTG 4 cut(s) 484, 715, 754, 964
VpaK11BI GGWCC 1 cut(s) 443
XapI RAATTY 7 cut(s) 37, 784, 895, 916, 936, 1093, 1120
XceI RCATGY 1 cut(s) 1109
XcmI CCANNNNNNNNNTGG 1 cut(s) 928
XmnI GAANNNNTTC 1 cut(s) 1183
XspI CTAG 3 cut(s) 26, 227, 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.