RchiOBHm_Chr2g0112241

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0012774
Physical Location & Seq
Reverse (-)
24135022 .. 24137190
2169 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48573

Sequence Viewer

Length: 1281 bp
ATGGGGTTTTCTCAGAGAAAGCTCCTCCCAGGAAACATCTTTAAGGATCCAGGTGAAGCCTTCTGTAAACCTAGTTGTGACCCAAATCTAAATAAACAAGGCTTGTGTGATGCATCTTGCCGCAAAATCTGCCCAACAGTCTGTAAATTTTCTGTGGGTGATAATGATAGTCTTCCAAATTTACCAGCTTTTCCTGACCCACCGCCGCCGCCGCCGCCGCCTGAGGTGGTTGATTCTAGACCAGACTATACTGGCCAGAAGCATCTGATCCAAACGAGTATGATAGTTGGGTCCTGTATGATCGGGGCTTCTCTTTTTCTCTGTATTGTTTTTGCAATTATGAGATACTATTCGAGGCGGAGAAATCCCAGAAGGAGAAGCCCGCCGATTCTGTTTGCTACGGCGGAAGATTTTCTTGATGAGGATCATGGGCCTGTGCTTGATCACCCCATTTGGTACATCCACACTGTGGGTCTGTCTCAGGCTTTGATCGATTCCATTACAGTTTGTAGGTATAGAAAAGATGAGGGTTTGATTGAGGGGACGGATTGTTCTGTTTGCTTGAGTGAGTTTGAGGAAGATGAGAGTCTCAGGCTTTTGCCAAAGTGTAGTCATGCTTTTCACATCCCTTGTATTGATACATGGTTGAGATCACATAAGAATTGCCCTCTTTGCCGCGCTCCGATTGTTTGTGGTGCTGCTAGTGATCAAGTGGGTGTCCCGGATTCGATTGCGAGTGGTTCGGGTTCTGGGGAGAGCATTCGGGTGGAGAATTTGGAGAGTGTTGAAGGGATTGGAAGAGGGACTAGTGAGGTTGGAGGTACAGATGATGAGAATGTCGGTGCAAATTCTGTTGATGGAAGGAGTGTTGAAAGTTCAAAGGTTTTGCCTAATTCTATTTTGGCTGAGGGAAGTTCTGATCCAAGAGCATTGAGCGATTTGGCGGATAATAGACGAGTGACTGAAGATGATGTACAGCCAATGAGGAGGTCAGTGTCGGTGGATTCAGTGTCTGCTTCAAGAATATATCATGCTGTGGCCAATGCTATTCCGGAACAAGGAAGTTCGAATTCCCAATTAGTACTTGTAAAGAATCCAAAGCTGGGGATTGTTCCGAAGCAGGACAGTGCAAGTTCTAGCCTATCCAAGCATTGGAGAAGTTCTTCAAATGGTCTTTCTCTGCAGATGGTGCCAATGGCAATGAAAAGGCCTTTCCCCTCTGGCAGGAAGCTTTTCTCATCGAAACACAGAAGGACTCTTAGCTCAATCCTTCCCTTATGA

Protein Analysis

426

Amino Acids

46.27

Weight (kDa)

6.56

Isoelectric Point (pI)

59.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 183 - 226 3.2e-14 Ring finger domain
zf-RING_11 PF17123 183 - 212 1.2e-10 RING-like zinc finger
zf-rbx1 PF12678 184 - 226 4.3e-09 RING-H2 zinc finger domain
zf-C3HC4 PF00097 184 - 225 1.6e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 184 - 225 1e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012774)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10380
fragaria_vesca FvH4_1g20164
malus_domestica MD01G1036900.v1.1 MD15G1317400.v1.1
prunus_persica Prupe.6G194300_v2.0.a1
pyrus_communis pycom01g06390 pycom15g27980
rosa_chinensis RchiOBHm_Chr2g0112241
rosa_laevigata RLG00000017962
rosa_multiflora Rmu_co8477273.1_g000001 Rmu_sc0001386.1_g000026
rosa_roxburghii Rroxscaffold_2G00131620
rosa_rugosa Rorug02G0179300
rosa_samantha Rh2AG231700 Rh2BG245200 Rh2CG236100 Rh2DG239600
rosa_wichuraiana Rw2G017980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1189
AccB7I CCANNNNNTGG 2 cut(s) 469, 1152
AccII CGCG 1 cut(s) 678
AccIII TCCGGA 1 cut(s) 1051
AclWI GGATC 5 cut(s) 41, 54, 262, 432, 914
AcoI YGGCCR 2 cut(s) 253, 1038
AcsI RAATTY 5 cut(s) 146, 178, 772, 847, 1069
AcuI CTGAAG 1 cut(s) 984
AdeI CACNNNGTG 1 cut(s) 469
AfaI GTAC 4 cut(s) 458, 823, 975, 1083
AfiI CCNNNNNNNGG 5 cut(s) 469, 1058, 1103, 1152, 1224
AgsI TTSAA 5 cut(s) 788, 872, 879, 1020, 1167
AhlI ACTAGT 1 cut(s) 806
AjnI CCWGG 2 cut(s) 28, 49
AloI GAACNNNNNNTCC 2 cut(s) 535, 567
AluBI AGCT 5 cut(s) 22, 188, 1102, 1231, 1263
AluI AGCT 5 cut(s) 22, 188, 1102, 1231, 1263
Alw26I GTCTC 2 cut(s) 483, 593
AlwI GGATC 5 cut(s) 41, 54, 262, 432, 914
AlwNI CAGNNNCTG 1 cut(s) 1013
Aor13HI TCCGGA 1 cut(s) 1051
AoxI GGCC 4 cut(s) 253, 431, 1038, 1208
ApeKI GCWGC 1 cut(s) 698
ApoI RAATTY 5 cut(s) 146, 178, 772, 847, 1069
Asp700I GAANNNNTTC 3 cut(s) 411, 1162, 1232
AspLEI GCGC 1 cut(s) 680
AspS9I GGNCC 2 cut(s) 291, 431
AsuC2I CCSGG 1 cut(s) 722
AsuHPI GGTGA 3 cut(s) 65, 170, 437
AsuII TTCGAA 1 cut(s) 1067
AvaII GGWCC 1 cut(s) 291
AxyI CCTNAGG 1 cut(s) 222
BalI TGGCCA 2 cut(s) 255, 1040
BamHI GGATCC 1 cut(s) 46
BanI GGYRCC 1 cut(s) 1189
BarI GAAGNNNNNNTAC 2 cut(s) 957, 989
BbsI GAAGAC 1 cut(s) 164
BbvCI CCTCAGC 1 cut(s) 906
BbvI GCAGC 1 cut(s) 685
BccI CCATC 2 cut(s) 851, 1180
BceAI ACGGC 1 cut(s) 417
BciT130I CCWGG 2 cut(s) 30, 51
BclI TGATCA 2 cut(s) 442, 706
BcnI CCSGG 1 cut(s) 722
BcoDI GTCTC 2 cut(s) 483, 593
BcuI ACTAGT 1 cut(s) 806
BfaI CTAG 5 cut(s) 72, 237, 702, 807, 1137
BfmI CTRYAG 1 cut(s) 1181
BisI GCNGC 8 cut(s) 121, 206, 209, 212, 215, 218, 676, 699
BlsI GCNGC 8 cut(s) 122, 207, 210, 213, 216, 219, 677, 700
BmcAI AGTACT 1 cut(s) 1083
Bme1390I CCNGG 3 cut(s) 30, 51, 722
Bme18I GGWCC 1 cut(s) 291
BmgT120I GGNCC 2 cut(s) 291, 431
BmiI GGNNCC 3 cut(s) 48, 292, 1191
BmrFI CCNGG 3 cut(s) 30, 51, 722
BmsI GCATC 3 cut(s) 100, 122, 271
BpiI GAAGAC 1 cut(s) 164
Bpu10I CCTNAGC 1 cut(s) 906
Bpu14I TTCGAA 1 cut(s) 1067
BpuEI CTTGAG 1 cut(s) 583
BpuMI CCSGG 1 cut(s) 722
Bsa29I ATCGAT 1 cut(s) 492
BsaBI GATNNNNATC 1 cut(s) 423
BsaJI CCNNGG 1 cut(s) 28
BsaWI WCCGGW 1 cut(s) 1051
Bsc4I CCNNNNNNNGG 5 cut(s) 469, 1058, 1103, 1152, 1224
Bse1I ACTGG 1 cut(s) 256
Bse21I CCTNAGG 1 cut(s) 222
Bse3DI GCAATG 1 cut(s) 1206
Bse8I GATNNNNATC 1 cut(s) 423
BseAI TCCGGA 1 cut(s) 1051
BseBI CCWGG 2 cut(s) 30, 51
BseCI ATCGAT 1 cut(s) 492
BseDI CCNNGG 1 cut(s) 28
BseGI GGATG 2 cut(s) 459, 624
BseJI GATNNNNATC 1 cut(s) 423
BseLI CCNNNNNNNGG 5 cut(s) 469, 1058, 1103, 1152, 1224
BseMI GCAATG 1 cut(s) 1206
BseMII CTCAG 5 cut(s) 26, 213, 494, 604, 897
BseNI ACTGG 1 cut(s) 256
BseRI GAGGAG 2 cut(s) 14, 1000
BseXI GCAGC 1 cut(s) 685
BseYI CCCAGC 1 cut(s) 1102
Bsh1236I CGCG 1 cut(s) 678
BshFI GGCC 4 cut(s) 255, 433, 1040, 1210
BshNI GGYRCC 1 cut(s) 1189
BshVI ATCGAT 1 cut(s) 492
BsiSI CCGG 2 cut(s) 722, 1052
BslFI GGGAC 3 cut(s) 556, 704, 817
BslI CCNNNNNNNGG 5 cut(s) 469, 1058, 1103, 1152, 1224
BsmAI GTCTC 2 cut(s) 483, 593
BsmFI GGGAC 3 cut(s) 556, 704, 817
BsmI GAATGC 1 cut(s) 759
BsnI GGCC 4 cut(s) 255, 433, 1040, 1210
Bsp119I TTCGAA 1 cut(s) 1067
Bsp13I TCCGGA 1 cut(s) 1051
Bsp1407I TGTACA 1 cut(s) 973
Bsp143I GATC 9 cut(s) 46, 267, 300, 424, 442, 489, 650, 706, 919
BspANI GGCC 4 cut(s) 255, 433, 1040, 1210
BspCNI CTCAG 5 cut(s) 25, 214, 493, 603, 898
BspDI ATCGAT 1 cut(s) 492
BspEI TCCGGA 1 cut(s) 1051
BspFNI CGCG 1 cut(s) 678
BspLI GGNNCC 3 cut(s) 48, 292, 1191
BspMAI CTGCAG 1 cut(s) 1185
BspPI GGATC 5 cut(s) 41, 54, 262, 432, 914
BspT104I TTCGAA 1 cut(s) 1067
BspT107I GGYRCC 1 cut(s) 1189
BsrDI GCAATG 1 cut(s) 1206
BsrGI TGTACA 1 cut(s) 973
BsrI ACTGG 1 cut(s) 256
BssECI CCNNGG 1 cut(s) 28
BssMI GATC 9 cut(s) 46, 267, 300, 424, 442, 489, 650, 706, 919
Bst2UI CCWGG 2 cut(s) 30, 51
Bst4CI ACNGT 4 cut(s) 139, 469, 505, 1127
Bst6I CTCTTC 1 cut(s) 793
BstAPI GCANNNNNTGC 2 cut(s) 129, 1189
BstAUI TGTACA 1 cut(s) 973
BstBI TTCGAA 1 cut(s) 1067
BstC8I GCNNGC 1 cut(s) 383
BstDEI CTNAG 6 cut(s) 12, 222, 480, 590, 906, 1259
BstENI CCTNNNNNAGG 1 cut(s) 1222
BstF5I GGATG 2 cut(s) 459, 624
BstFNI CGCG 1 cut(s) 678
BstHHI GCGC 1 cut(s) 680
BstKTI GATC 9 cut(s) 49, 270, 303, 427, 445, 492, 653, 709, 922
BstMAI GTCTC 2 cut(s) 483, 593
BstMBI GATC 9 cut(s) 46, 267, 300, 424, 442, 489, 650, 706, 919
BstMWI GCNNNNNNNGC 6 cut(s) 129, 211, 214, 217, 672, 1189
BstNI CCWGG 2 cut(s) 30, 51
BstSCI CCNGG 3 cut(s) 28, 49, 720
BstSFI CTRYAG 1 cut(s) 1181
BstUI CGCG 1 cut(s) 678
BstV1I GCAGC 1 cut(s) 685
BstV2I GAAGAC 1 cut(s) 164
BstX2I RGATCY 1 cut(s) 46
BstYI RGATCY 1 cut(s) 46
Bsu15I ATCGAT 1 cut(s) 492
Bsu36I CCTNAGG 1 cut(s) 222
BsuRI GGCC 4 cut(s) 255, 433, 1040, 1210
BsuTUI ATCGAT 1 cut(s) 492
BtsCI GGATG 2 cut(s) 459, 624
BtsIMutI CAGTG 4 cut(s) 465, 999, 1014, 1132
Cac8I GCNNGC 1 cut(s) 383
CaiI CAGNNNCTG 1 cut(s) 1013
CfoI GCGC 1 cut(s) 680
Cfr13I GGNCC 2 cut(s) 291, 431
ClaI ATCGAT 1 cut(s) 492
Csp6I GTAC 4 cut(s) 457, 822, 974, 1082
CviAII CATG 4 cut(s) 428, 614, 642, 1031
CviQI GTAC 4 cut(s) 457, 822, 974, 1082
DdeI CTNAG 6 cut(s) 12, 222, 480, 590, 906, 1259
DpnI GATC 9 cut(s) 48, 269, 302, 426, 444, 491, 652, 708, 921
DpnII GATC 9 cut(s) 46, 267, 300, 424, 442, 489, 650, 706, 919
DraIII CACNNNGTG 1 cut(s) 469
EaeI YGGCCR 2 cut(s) 253, 1038
Eam1104I CTCTTC 1 cut(s) 793
EarI CTCTTC 1 cut(s) 793
EciI GGCGGA 3 cut(s) 373, 419, 959
Eco147I AGGCCT 1 cut(s) 1210
Eco47I GGWCC 1 cut(s) 291
Eco57I CTGAAG 1 cut(s) 984
Eco81I CCTNAGG 1 cut(s) 222
EcoNI CCTNNNNNAGG 1 cut(s) 1222
EcoO109I RGGNCCY 1 cut(s) 291
EcoRI GAATTC 1 cut(s) 1069
EcoRII CCWGG 2 cut(s) 28, 49
EcoT22I ATGCAT 1 cut(s) 115
FaeI CATG 4 cut(s) 431, 617, 645, 1034
FalI AAGNNNNNCTT 2 cut(s) 399, 431
FaqI GGGAC 3 cut(s) 556, 704, 817
FatI CATG 4 cut(s) 427, 613, 641, 1030
FauI CCCGC 1 cut(s) 390
FbaI TGATCA 2 cut(s) 442, 706
Fnu4HI GCNGC 8 cut(s) 121, 206, 209, 212, 215, 218, 676, 699
FokI GGATG 2 cut(s) 446, 611
Fsp4HI GCNGC 8 cut(s) 121, 206, 209, 212, 215, 218, 676, 699
FspBI CTAG 5 cut(s) 72, 237, 702, 807, 1137
GlaI GCGC 1 cut(s) 679
GluI GCNGC 8 cut(s) 121, 206, 209, 212, 215, 218, 676, 699
GsaI CCCAGC 1 cut(s) 1106
HaeIII GGCC 4 cut(s) 255, 433, 1040, 1210
HapII CCGG 2 cut(s) 722, 1052
HhaI GCGC 1 cut(s) 680
Hin1II CATG 4 cut(s) 431, 617, 645, 1034
Hin6I GCGC 1 cut(s) 678
HinP1I GCGC 1 cut(s) 678
HindIII AAGCTT 1 cut(s) 1229
HinfI GANTC 8 cut(s) 233, 388, 494, 586, 725, 1004, 1093, 1255
HpaII CCGG 2 cut(s) 722, 1052
HphI GGTGA 3 cut(s) 65, 170, 437
Hpy166II GTNNAC 1 cut(s) 68
Hpy188I TCNGA 5 cut(s) 15, 267, 684, 919, 1116
Hpy188III TCNNGA 5 cut(s) 194, 237, 416, 1020, 1052
Hpy8I GTNNAC 1 cut(s) 68
HpyAV CCTTC 6 cut(s) 70, 366, 782, 855, 1245, 1280
HpyCH4III ACNGT 4 cut(s) 139, 469, 505, 1127
HpyCH4V TGCA 5 cut(s) 113, 335, 845, 1130, 1183
HpyF10VI GCNNNNNNNGC 6 cut(s) 129, 211, 214, 217, 672, 1189
HpyF3I CTNAG 6 cut(s) 12, 222, 480, 590, 906, 1259
Hsp92II CATG 4 cut(s) 431, 617, 645, 1034
HspAI GCGC 1 cut(s) 678
Kpn2I TCCGGA 1 cut(s) 1051
Ksp22I TGATCA 2 cut(s) 442, 706
Kzo9I GATC 9 cut(s) 46, 267, 300, 424, 442, 489, 650, 706, 919
LmnI GCTCC 2 cut(s) 27, 685
Lsp1109I GCAGC 1 cut(s) 685
LweI GCATC 3 cut(s) 100, 122, 271
MaeI CTAG 5 cut(s) 72, 237, 702, 807, 1137
MaeIII GTNAC 2 cut(s) 77, 958
MalI GATC 9 cut(s) 48, 269, 302, 426, 444, 491, 652, 708, 921
MboI GATC 9 cut(s) 46, 267, 300, 424, 442, 489, 650, 706, 919
MboII GAAGA 6 cut(s) 164, 419, 590, 810, 977, 1155
MflI RGATCY 1 cut(s) 46
MlsI TGGCCA 2 cut(s) 255, 1040
MluCI AATT 9 cut(s) 146, 178, 336, 661, 772, 847, 892, 1069, 1076
MluNI TGGCCA 2 cut(s) 255, 1040
MlyI GAGTC 2 cut(s) 595, 1249
MmeI TCCRAC 1 cut(s) 796
Mox20I TGGCCA 2 cut(s) 255, 1040
Mph1103I ATGCAT 1 cut(s) 115
MroI TCCGGA 1 cut(s) 1051
MroXI GAANNNNTTC 3 cut(s) 411, 1162, 1232
MscI TGGCCA 2 cut(s) 255, 1040
MseI TTAA 1 cut(s) 42
MslI CAYNNNNRTG 1 cut(s) 764
Msp20I TGGCCA 2 cut(s) 255, 1040
MspI CCGG 2 cut(s) 722, 1052
MspR9I CCNGG 3 cut(s) 30, 51, 722
Mva1269I GAATGC 1 cut(s) 759
MvaI CCWGG 2 cut(s) 30, 51
MvnI CGCG 1 cut(s) 678
MwoI GCNNNNNNNGC 6 cut(s) 129, 211, 214, 217, 672, 1189
NciI CCSGG 1 cut(s) 722
NdeII GATC 9 cut(s) 46, 267, 300, 424, 442, 489, 650, 706, 919
NlaIII CATG 4 cut(s) 431, 617, 645, 1034
NlaIV GGNNCC 3 cut(s) 48, 292, 1191
NmuCI GTSAC 2 cut(s) 77, 958
NsiI ATGCAT 1 cut(s) 115
NspV TTCGAA 1 cut(s) 1067
PceI AGGCCT 1 cut(s) 1210
PctI GAATGC 1 cut(s) 759
PdmI GAANNNNTTC 3 cut(s) 411, 1162, 1232
PfeI GAWTC 6 cut(s) 233, 388, 494, 725, 1004, 1093
PflMI CCANNNNNTGG 2 cut(s) 469, 1152
PfoI TCCNGGA 1 cut(s) 720
PkrI GCNGC 8 cut(s) 122, 207, 210, 213, 216, 219, 677, 700
PleI GAGTC 2 cut(s) 594, 1249
PpsI GAGTC 2 cut(s) 594, 1249
PpuMI RGGWCCY 1 cut(s) 291
Psp5II RGGWCCY 1 cut(s) 291
Psp6I CCWGG 2 cut(s) 28, 49
PspFI CCCAGC 1 cut(s) 1102
PspGI CCWGG 2 cut(s) 28, 49
PspN4I GGNNCC 3 cut(s) 48, 292, 1191
PspPI GGNCC 2 cut(s) 291, 431
PspPPI RGGWCCY 1 cut(s) 291
PstI CTGCAG 1 cut(s) 1185
PstNI CAGNNNCTG 1 cut(s) 1013
PsuI RGATCY 1 cut(s) 46
RsaI GTAC 4 cut(s) 458, 823, 975, 1083
RsaNI GTAC 4 cut(s) 457, 822, 974, 1082
RseI CAYNNNNRTG 1 cut(s) 764
SaqAI TTAA 1 cut(s) 42
SatI GCNGC 8 cut(s) 121, 206, 209, 212, 215, 218, 676, 699
Sau3AI GATC 9 cut(s) 46, 267, 300, 424, 442, 489, 650, 706, 919
Sau96I GGNCC 2 cut(s) 291, 431
ScaI AGTACT 1 cut(s) 1083
SchI GAGTC 2 cut(s) 595, 1249
ScrFI CCNGG 3 cut(s) 30, 51, 722
SfaNI GCATC 3 cut(s) 100, 122, 271
SfcI CTRYAG 1 cut(s) 1181
SfuI TTCGAA 1 cut(s) 1067
SinI GGWCC 1 cut(s) 291
SmiMI CAYNNNNRTG 1 cut(s) 764
SmlI CTYRAG 1 cut(s) 562
SmoI CTYRAG 1 cut(s) 562
SpeI ACTAGT 1 cut(s) 806
Sse9I AATT 9 cut(s) 146, 178, 336, 661, 772, 847, 892, 1069, 1076
SseBI AGGCCT 1 cut(s) 1210
SspMI CTAG 5 cut(s) 72, 237, 702, 807, 1137
StuI AGGCCT 1 cut(s) 1210
StyD4I CCNGG 3 cut(s) 28, 49, 720
TaaI ACNGT 4 cut(s) 139, 469, 505, 1127
TaqI TCGA 5 cut(s) 353, 492, 728, 1067, 1241
TasI AATT 9 cut(s) 146, 178, 336, 661, 772, 847, 892, 1069, 1076
TatI WGTACW 2 cut(s) 973, 1081
TauI GCSGC 7 cut(s) 123, 208, 211, 214, 217, 220, 678
TfiI GAWTC 6 cut(s) 233, 388, 494, 725, 1004, 1093
Tru1I TTAA 1 cut(s) 42
Tru9I TTAA 1 cut(s) 42
TscAI CASTG 4 cut(s) 472, 999, 1014, 1132
TseFI GTSAC 2 cut(s) 77, 958
TseI GCWGC 1 cut(s) 698
Tsp45I GTSAC 2 cut(s) 77, 958
TspDTI ATGAA 1 cut(s) 1217
TspGWI ACGGA 1 cut(s) 560
TspRI CASTG 4 cut(s) 472, 999, 1014, 1132
Van91I CCANNNNNTGG 2 cut(s) 469, 1152
VpaK11BI GGWCC 1 cut(s) 291
XagI CCTNNNNNAGG 1 cut(s) 1222
XapI RAATTY 5 cut(s) 146, 178, 772, 847, 1069
XbaI TCTAGA 1 cut(s) 236
XmnI GAANNNNTTC 3 cut(s) 411, 1162, 1232
XspI CTAG 5 cut(s) 72, 237, 702, 807, 1137
ZrmI AGTACT 1 cut(s) 1083
Zsp2I ATGCAT 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.