Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0012774

Physical Location & Seq

Genomic Coordinates Reverse (-)

32740480 .. 32741357

Gene Structure 878 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1317400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 798 bp

ATGACTATCACAATTTGTACGATTGGGGCTGCTCTTCTTCTTGCTATTGTTTTCTCAATTGTTAGATTTTACCGCCGCCGCCGCCGCAACAATTCGTCGAGGAGTAGGTCCTTACCGATTTTGTTTGATACCCAACAAGATTTTATTGATGAAGATCACGGTCCTGTTCTTGATCATCCCATTTGGTACATACAGACTGTTGGTCTCCCACAATCTGTGATTGACTCTATAACTGTCTGCAAGTTTCGAAAAGATGAGGGTTTGATTGAGGGAACGGATTGTTCTGTTTGCTTGAATGAGTTTGAAGAAGATGAGAGTCTTAGGCTTTTGCCGAAGTGCAGTCATGCCTTTCACATCCCTTGTATTGATACCTGGTTGAGATCACATAAGAATTGCCCGCTTTGCCGCGCTCCGATTGTTTATGACACTGGTAGTGCTCAGGTGAGTGTCCCGGAGCCTGTTTCGAGTGGTTCAGGTTCAAGGGATAGCGGTGAGGTGGAGAATTTGGAGGGTGATGGAGGGGCTGAAAGTGTTGGAAGTTGGACTAATGAGGTTGGGGCTGGAGAGGTTGGTAATGTTTCTGTAATTCCAATTGAGGGAAGGGCCGCTGAAAGTTCGAGGAAGTTTTTGCGAAATTCCATTGTGCCTGTGGGAAGTTGTGATCCGAGAGCATTCAGTGATTTGACTGACAATCGGCGTGTTACTGAAGAGGATACTCAACCAATGAGGAGATCAGTTTCGATGGATTCAATGTCTGCTTTGAGAATATATCGCGATGTGGCGAAGTTCAAGTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

266

Amino Acids

29.39

Weight (kDa)

5.35

Isoelectric Point (pI)

62.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	93 - 136	1.8e-14	Ring finger domain
zf-RING_11	PF17123	93 - 122	5.5e-11	RING-like zinc finger
zf-C3HC4_2	PF13923	94 - 135	2.9e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	94 - 135	6.5e-08	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	100 - 136	7.7e-09	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0012774)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10380
fragaria_vesca	FvH4_1g20164
malus_domestica	MD01G1036900.v1.1 MD15G1317400.v1.1
prunus_persica	Prupe.6G194300_v2.0.a1
pyrus_communis	pycom01g06390 pycom15g27980
rosa_chinensis	RchiOBHm_Chr2g0112241
rosa_laevigata	RLG00000017962
rosa_multiflora	Rmu_co8477273.1_g000001 Rmu_sc0001386.1_g000026
rosa_roxburghii	Rroxscaffold_2G00131620
rosa_rugosa	Rorug02G0179300
rosa_samantha	Rh2AG231700 Rh2BG245200 Rh2CG236100 Rh2DG239600
rosa_wichuraiana	Rw2G017980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	408, 774
AciI	CCGC	9 cut(s)	73, 76, 79, 82, 85, 398, 406, 489, 606
AclWI	GGATC	1 cut(s)	656
AcsI	RAATTY	2 cut(s)	502, 634
AcuI	CTGAAG	1 cut(s)	726
AfaI	GTAC	2 cut(s)	19, 188
AfiI	CCNNNNNNNGG	1 cut(s)	596
AgsI	TTSAA	5 cut(s)	295, 305, 480, 750, 790
AhdI	GACNNNNNGTC	1 cut(s)	201
AjnI	CCWGG	1 cut(s)	371
Alw21I	GWGCWC	1 cut(s)	439
Alw26I	GTCTC	1 cut(s)	209
AlwI	GGATC	1 cut(s)	656
AoxI	GGCC	1 cut(s)	603
ApeKI	GCWGC	1 cut(s)	29
ApoI	RAATTY	2 cut(s)	502, 634
AspLEI	GCGC	1 cut(s)	410
AspS9I	GGNCC	3 cut(s)	108, 161, 603
AsuC2I	CCSGG	1 cut(s)	452
AsuHPI	GGTGA	3 cut(s)	454, 503, 524
AsuII	TTCGAA	1 cut(s)	247
AvaII	GGWCC	2 cut(s)	108, 161
Bbv12I	GWGCWC	1 cut(s)	439
BbvI	GCAGC	1 cut(s)	16
BccI	CCATC	2 cut(s)	509, 736
BciT130I	CCWGG	1 cut(s)	373
BciVI	GTATCC	1 cut(s)	706
BclI	TGATCA	1 cut(s)	172
BcnI	CCSGG	1 cut(s)	452
BcoDI	GTCTC	1 cut(s)	209
BfaI	CTAG	1 cut(s)	796
BfuI	GTATCC	1 cut(s)	706
BisI	GCNGC	7 cut(s)	30, 76, 79, 82, 85, 406, 606
BlsI	GCNGC	7 cut(s)	31, 77, 80, 83, 86, 407, 607
Bme1390I	CCNGG	2 cut(s)	373, 452
Bme18I	GGWCC	2 cut(s)	108, 161
BmeRI	GACNNNNNGTC	1 cut(s)	201
BmgT120I	GGNCC	3 cut(s)	108, 161, 603
BmiI	GGNNCC	1 cut(s)	456
BmrFI	CCNGG	2 cut(s)	373, 452
BpmI	CTGGAG	1 cut(s)	582
Bpu10I	CCTNAGC	1 cut(s)	438
Bpu14I	TTCGAA	1 cut(s)	247
BpuMI	CCSGG	1 cut(s)	452
BsaBI	GATNNNNATC	1 cut(s)	153
BsaI	GGTCTC	1 cut(s)	209
Bsc4I	CCNNNNNNNGG	1 cut(s)	596
Bse1I	ACTGG	1 cut(s)	433
Bse8I	GATNNNNATC	1 cut(s)	153
BseBI	CCWGG	1 cut(s)	373
BseGI	GGATG	2 cut(s)	175, 354
BseJI	GATNNNNATC	1 cut(s)	153
BseLI	CCNNNNNNNGG	1 cut(s)	596
BseMII	CTCAG	1 cut(s)	452
BseNI	ACTGG	1 cut(s)	433
BseRI	GAGGAG	2 cut(s)	115, 742
BseXI	GCAGC	1 cut(s)	16
BsgI	GTGCAG	1 cut(s)	358
Bsh1236I	CGCG	2 cut(s)	408, 774
BshFI	GGCC	1 cut(s)	605
BsiHKAI	GWGCWC	1 cut(s)	439
BsiSI	CCGG	1 cut(s)	452
BslFI	GGGAC	1 cut(s)	434
BslI	CCNNNNNNNGG	1 cut(s)	596
BsmAI	GTCTC	1 cut(s)	209
BsmFI	GGGAC	1 cut(s)	434
BsmI	GAATGC	1 cut(s)	671
BsnI	GGCC	1 cut(s)	605
Bso31I	GGTCTC	1 cut(s)	209
Bsp119I	TTCGAA	1 cut(s)	247
Bsp1286I	GDGCHC	1 cut(s)	439
Bsp143I	GATC	5 cut(s)	154, 172, 380, 661, 731
Bsp68I	TCGCGA	1 cut(s)	774
BspACI	CCGC	9 cut(s)	73, 76, 79, 82, 85, 398, 406, 489, 606
BspANI	GGCC	1 cut(s)	605
BspCNI	CTCAG	1 cut(s)	451
BspFNI	CGCG	2 cut(s)	408, 774
BspLI	GGNNCC	1 cut(s)	456
BspPI	GGATC	1 cut(s)	656
BspQI	GCTCTTC	1 cut(s)	39
BspT104I	TTCGAA	1 cut(s)	247
BspTNI	GGTCTC	1 cut(s)	209
BsrI	ACTGG	1 cut(s)	433
BssMI	GATC	5 cut(s)	154, 172, 380, 661, 731
Bst2UI	CCWGG	1 cut(s)	373
Bst4CI	ACNGT	3 cut(s)	161, 199, 235
Bst6I	CTCTTC	2 cut(s)	39, 702
BstBI	TTCGAA	1 cut(s)	247
BstC8I	GCNNGC	1 cut(s)	398
BstDEI	CTNAG	2 cut(s)	320, 438
BstF5I	GGATG	2 cut(s)	175, 354
BstFNI	CGCG	2 cut(s)	408, 774
BstHHI	GCGC	1 cut(s)	410
BstKTI	GATC	5 cut(s)	157, 175, 383, 664, 734
BstMAI	GTCTC	1 cut(s)	209
BstMBI	GATC	5 cut(s)	154, 172, 380, 661, 731
BstMWI	GCNNNNNNNGC	3 cut(s)	81, 84, 402
BstNI	CCWGG	1 cut(s)	373
BstSCI	CCNGG	2 cut(s)	371, 450
BstUI	CGCG	2 cut(s)	408, 774
BstV1I	GCAGC	1 cut(s)	16
BsuI	GTATCC	1 cut(s)	706
BsuRI	GGCC	1 cut(s)	605
BtgZI	GCGATG	1 cut(s)	789
BtsCI	GGATG	2 cut(s)	175, 354
BtsIMutI	CAGTG	2 cut(s)	426, 682
BtuMI	TCGCGA	1 cut(s)	774
Cac8I	GCNNGC	1 cut(s)	398
CfoI	GCGC	1 cut(s)	410
Cfr13I	GGNCC	3 cut(s)	108, 161, 603
CsiI	ACCWGGT	1 cut(s)	371
Csp6I	GTAC	2 cut(s)	18, 187
CviAII	CATG	1 cut(s)	344
CviJI	RGCY	6 cut(s)	29, 325, 457, 524, 560, 605
CviKI_1	RGCY	6 cut(s)	29, 325, 457, 524, 560, 605
CviQI	GTAC	2 cut(s)	18, 187
DdeI	CTNAG	2 cut(s)	320, 438
DpnI	GATC	5 cut(s)	156, 174, 382, 663, 733
DpnII	GATC	5 cut(s)	154, 172, 380, 661, 731
DriI	GACNNNNNGTC	1 cut(s)	201
Eam1104I	CTCTTC	2 cut(s)	39, 702
Eam1105I	GACNNNNNGTC	1 cut(s)	201
EarI	CTCTTC	2 cut(s)	39, 702
Eco31I	GGTCTC	1 cut(s)	209
Eco47I	GGWCC	2 cut(s)	108, 161
Eco57I	CTGAAG	1 cut(s)	726
EcoO109I	RGGNCCY	1 cut(s)	108
EcoRII	CCWGG	1 cut(s)	371
FaeI	CATG	1 cut(s)	347
FaiI	YATR	6 cut(s)	191, 230, 345, 387, 423, 769
FaqI	GGGAC	1 cut(s)	434
FatI	CATG	1 cut(s)	343
FauI	CCCGC	1 cut(s)	405
FbaI	TGATCA	1 cut(s)	172
Fnu4HI	GCNGC	7 cut(s)	30, 76, 79, 82, 85, 406, 606
FokI	GGATG	2 cut(s)	162, 341
Fsp4HI	GCNGC	7 cut(s)	30, 76, 79, 82, 85, 406, 606
FspBI	CTAG	1 cut(s)	796
GlaI	GCGC	1 cut(s)	409
GluI	GCNGC	7 cut(s)	30, 76, 79, 82, 85, 406, 606
GsuI	CTGGAG	1 cut(s)	582
HaeIII	GGCC	1 cut(s)	605
HapII	CCGG	1 cut(s)	452
HhaI	GCGC	1 cut(s)	410
Hin1II	CATG	1 cut(s)	347
Hin6I	GCGC	1 cut(s)	408
HinP1I	GCGC	1 cut(s)	408
HinfI	GANTC	3 cut(s)	224, 316, 746
HpaII	CCGG	1 cut(s)	452
HphI	GGTGA	3 cut(s)	454, 503, 524
Hpy188I	TCNGA	2 cut(s)	414, 666
Hpy188III	TCNNGA	2 cut(s)	170, 773
Hpy99I	CGWCG	1 cut(s)	100
HpyAV	CCTTC	1 cut(s)	594
HpyCH4III	ACNGT	3 cut(s)	161, 199, 235
HpyCH4V	TGCA	2 cut(s)	240, 339
HpyF10VI	GCNNNNNNNGC	3 cut(s)	81, 84, 402
HpyF3I	CTNAG	2 cut(s)	320, 438
Hsp92II	CATG	1 cut(s)	347
HspAI	GCGC	1 cut(s)	408
Ksp22I	TGATCA	1 cut(s)	172
Kzo9I	GATC	5 cut(s)	154, 172, 380, 661, 731
LguI	GCTCTTC	1 cut(s)	39
LmnI	GCTCC	2 cut(s)	415, 454
Lsp1109I	GCAGC	1 cut(s)	16
MabI	ACCWGGT	1 cut(s)	371
MaeI	CTAG	1 cut(s)	796
MaeIII	GTNAC	1 cut(s)	700
MalI	GATC	5 cut(s)	156, 174, 382, 663, 733
MboI	GATC	5 cut(s)	154, 172, 380, 661, 731
MboII	GAAGA	6 cut(s)	26, 29, 164, 317, 320, 719
MfeI	CAATTG	2 cut(s)	57, 591
MhlI	GDGCHC	1 cut(s)	439
MluCI	AATT	8 cut(s)	12, 57, 91, 391, 502, 585, 591, 634
MlyI	GAGTC	2 cut(s)	218, 325
MmeI	TCCRAC	2 cut(s)	514, 521
MspA1I	CMGCKG	1 cut(s)	608
MspI	CCGG	1 cut(s)	452
MspR9I	CCNGG	2 cut(s)	373, 452
MunI	CAATTG	2 cut(s)	57, 591
Mva1269I	GAATGC	1 cut(s)	671
MvaI	CCWGG	1 cut(s)	373
MvnI	CGCG	2 cut(s)	408, 774
MwoI	GCNNNNNNNGC	3 cut(s)	81, 84, 402
NciI	CCSGG	1 cut(s)	452
NdeII	GATC	5 cut(s)	154, 172, 380, 661, 731
NlaIII	CATG	1 cut(s)	347
NlaIV	GGNNCC	1 cut(s)	456
NruI	TCGCGA	1 cut(s)	774
NspV	TTCGAA	1 cut(s)	247
PciSI	GCTCTTC	1 cut(s)	39
PctI	GAATGC	1 cut(s)	671
PfeI	GAWTC	1 cut(s)	746
PfoI	TCCNGGA	1 cut(s)	450
PkrI	GCNGC	7 cut(s)	31, 77, 80, 83, 86, 407, 607
PleI	GAGTC	2 cut(s)	218, 324
PpsI	GAGTC	2 cut(s)	218, 324
PpuMI	RGGWCCY	1 cut(s)	108
Psp5II	RGGWCCY	1 cut(s)	108
Psp6I	CCWGG	1 cut(s)	371
PspGI	CCWGG	1 cut(s)	371
PspN4I	GGNNCC	1 cut(s)	456
PspPI	GGNCC	3 cut(s)	108, 161, 603
PspPPI	RGGWCCY	1 cut(s)	108
RruI	TCGCGA	1 cut(s)	774
RsaI	GTAC	2 cut(s)	19, 188
RsaNI	GTAC	2 cut(s)	18, 187
SapI	GCTCTTC	1 cut(s)	39
SatI	GCNGC	7 cut(s)	30, 76, 79, 82, 85, 406, 606
Sau3AI	GATC	5 cut(s)	154, 172, 380, 661, 731
Sau96I	GGNCC	3 cut(s)	108, 161, 603
SchI	GAGTC	2 cut(s)	218, 325
ScrFI	CCNGG	2 cut(s)	373, 452
SduI	GDGCHC	1 cut(s)	439
SetI	ASST	7 cut(s)	110, 374, 444, 478, 498, 555, 570
SexAI	ACCWGGT	1 cut(s)	371
SfuI	TTCGAA	1 cut(s)	247
SinI	GGWCC	2 cut(s)	108, 161
Sse9I	AATT	8 cut(s)	12, 57, 91, 391, 502, 585, 591, 634
SsiI	CCGC	9 cut(s)	73, 76, 79, 82, 85, 398, 406, 489, 606
SspMI	CTAG	1 cut(s)	796
StyD4I	CCNGG	2 cut(s)	371, 450
TaaI	ACNGT	3 cut(s)	161, 199, 235
TaqI	TCGA	5 cut(s)	98, 247, 464, 617, 740
TasI	AATT	8 cut(s)	12, 57, 91, 391, 502, 585, 591, 634
TauI	GCSGC	6 cut(s)	78, 81, 84, 87, 408, 608
TfiI	GAWTC	1 cut(s)	746
TscAI	CASTG	2 cut(s)	433, 682
TseI	GCWGC	1 cut(s)	29
TspDTI	ATGAA	1 cut(s)	165
TspGWI	ACGGA	1 cut(s)	290
TspRI	CASTG	2 cut(s)	433, 682
VpaK11BI	GGWCC	2 cut(s)	108, 161
XapI	RAATTY	2 cut(s)	502, 634
XcmI	CCANNNNNNNNNTGG	1 cut(s)	646
XspI	CTAG	1 cut(s)	796

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.