MD01G1044000.v1.1

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012210
Physical Location & Seq
Forward (+)
14337433 .. 14337714
282 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1044000.v1.1.491

Sequence Viewer

Length: 282 bp
ATGGCCACCACCTTCGCCGCCCAGTCGTTCCCAGTCTTCGATCCTAATCGAGCTTCTAGGATGGTCCAGAACCCACCGGGCCCCACTTTTGGAGATGGGTTAAGCAGGTTGAGCGGCACTGGCGCTGGACCCGTCGGTTCCACAATGCAGATTGAGTCACGGTTGGAGATGGGGGGTCGGATTTGGGTCATGCACTTTCTTACCCACCATGTGGAGGCAAAATCTAGGTGTGAAGTGTACATTGCTGTGAGTGATGAATCTGTGTTGGCTTTTCACTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

94

Amino Acids

10.08

Weight (kDa)

6.39

Isoelectric Point (pI)

45.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 96
AccB7I CCANNNNNTGG 1 cut(s) 211
AccBSI CCGCTC 1 cut(s) 114
AciI CCGC 2 cut(s) 18, 114
AclWI GGATC 1 cut(s) 35
AcoI YGGCCR 1 cut(s) 3
AdeI CACNNNGTG 1 cut(s) 211
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 3 cut(s) 89, 211, 214
AluBI AGCT 1 cut(s) 53
AluI AGCT 1 cut(s) 53
AlwI GGATC 1 cut(s) 35
AoxI GGCC 2 cut(s) 3, 79
ApaI GGGCCC 1 cut(s) 83
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 4 cut(s) 64, 79, 80, 128
AsuC2I CCSGG 1 cut(s) 78
AvaII GGWCC 2 cut(s) 64, 128
BaeGI GKGCMC 1 cut(s) 83
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 83
BbsI GAAGAC 1 cut(s) 28
BccI CCATC 3 cut(s) 55, 89, 163
BcnI CCSGG 1 cut(s) 78
BfaI CTAG 2 cut(s) 57, 225
BfoI RGCGCY 1 cut(s) 126
BfuAI ACCTGC 1 cut(s) 96
BisI GCNGC 2 cut(s) 18, 115
BlsI GCNGC 2 cut(s) 19, 116
Bme1390I CCNGG 1 cut(s) 78
Bme18I GGWCC 2 cut(s) 64, 128
BmgT120I GGNCC 4 cut(s) 64, 79, 80, 128
BmiI GGNNCC 4 cut(s) 81, 82, 130, 139
BmrFI CCNGG 1 cut(s) 78
BmrI ACTGGG 2 cut(s) 16, 26
BmuI ACTGGG 2 cut(s) 16, 26
BpiI GAAGAC 1 cut(s) 28
BpuMI CCSGG 1 cut(s) 78
BsaBI GATNNNNATC 1 cut(s) 45
Bsc4I CCNNNNNNNGG 3 cut(s) 89, 211, 214
Bse1I ACTGG 3 cut(s) 22, 32, 124
Bse3DI GCAATG 1 cut(s) 240
Bse8I GATNNNNATC 1 cut(s) 45
BseGI GGATG 1 cut(s) 66
BseJI GATNNNNATC 1 cut(s) 45
BseLI CCNNNNNNNGG 3 cut(s) 89, 211, 214
BseMI GCAATG 1 cut(s) 240
BseNI ACTGG 3 cut(s) 22, 32, 124
BseSI GKGCMC 1 cut(s) 83
BshFI GGCC 2 cut(s) 5, 81
BsiSI CCGG 1 cut(s) 77
BslI CCNNNNNNNGG 3 cut(s) 89, 211, 214
BsnI GGCC 2 cut(s) 5, 81
Bsp120I GGGCCC 1 cut(s) 79
Bsp1286I GDGCHC 1 cut(s) 83
Bsp1407I TGTACA 1 cut(s) 237
Bsp143I GATC 1 cut(s) 40
BspACI CCGC 2 cut(s) 18, 114
BspANI GGCC 2 cut(s) 5, 81
BspLI GGNNCC 4 cut(s) 81, 82, 130, 139
BspMI ACCTGC 1 cut(s) 96
BspPI GGATC 1 cut(s) 35
BsrBI CCGCTC 1 cut(s) 114
BsrDI GCAATG 1 cut(s) 240
BsrGI TGTACA 1 cut(s) 237
BsrI ACTGG 3 cut(s) 22, 32, 124
BssMI GATC 1 cut(s) 40
Bst4CI ACNGT 2 cut(s) 162, 278
BstAUI TGTACA 1 cut(s) 237
BstF5I GGATG 1 cut(s) 66
BstH2I RGCGCY 1 cut(s) 126
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 1 cut(s) 43
BstMBI GATC 1 cut(s) 40
BstMWI GCNNNNNNNGC 2 cut(s) 111, 120
BstSCI CCNGG 1 cut(s) 76
BstSLI GKGCMC 1 cut(s) 83
BstV2I GAAGAC 1 cut(s) 28
BsuRI GGCC 2 cut(s) 5, 81
BtsCI GGATG 1 cut(s) 66
BtsIMutI CAGTG 2 cut(s) 117, 274
BveI ACCTGC 1 cut(s) 96
CfoI GCGC 1 cut(s) 125
Cfr13I GGNCC 4 cut(s) 64, 79, 80, 128
Csp6I GTAC 1 cut(s) 238
CviAII CATG 2 cut(s) 190, 209
CviJI RGCY 4 cut(s) 5, 53, 81, 269
CviKI_1 RGCY 4 cut(s) 5, 53, 81, 269
CviQI GTAC 1 cut(s) 238
DpnI GATC 1 cut(s) 42
DpnII GATC 1 cut(s) 40
DraIII CACNNNGTG 1 cut(s) 211
EaeI YGGCCR 1 cut(s) 3
Eco24I GRGCYC 1 cut(s) 83
Eco47I GGWCC 2 cut(s) 64, 128
EcoO109I RGGNCCY 1 cut(s) 80
EcoT38I GRGCYC 1 cut(s) 83
FaeI CATG 2 cut(s) 193, 212
FaiI YATR 2 cut(s) 191, 210
FatI CATG 2 cut(s) 189, 208
Fnu4HI GCNGC 2 cut(s) 18, 115
FokI GGATG 1 cut(s) 73
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 2 cut(s) 18, 115
FspBI CTAG 2 cut(s) 57, 225
GlaI GCGC 1 cut(s) 124
GluI GCNGC 2 cut(s) 18, 115
HaeII RGCGCY 1 cut(s) 126
HaeIII GGCC 2 cut(s) 5, 81
HapII CCGG 1 cut(s) 77
HhaI GCGC 1 cut(s) 125
Hin1II CATG 2 cut(s) 193, 212
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HinfI GANTC 2 cut(s) 155, 257
HpaII CCGG 1 cut(s) 77
Hpy166II GTNNAC 1 cut(s) 238
Hpy188I TCNGA 1 cut(s) 180
Hpy188III TCNNGA 1 cut(s) 67
Hpy8I GTNNAC 1 cut(s) 238
Hpy99I CGWCG 1 cut(s) 137
HpyAV CCTTC 1 cut(s) 22
HpyCH4III ACNGT 2 cut(s) 162, 278
HpyCH4V TGCA 2 cut(s) 148, 193
HpyF10VI GCNNNNNNNGC 2 cut(s) 111, 120
Hsp92II CATG 2 cut(s) 193, 212
HspAI GCGC 1 cut(s) 123
Kzo9I GATC 1 cut(s) 40
LpnPI CCDG 7 cut(s) 35, 45, 80, 90, 91, 105, 111
MaeI CTAG 2 cut(s) 57, 225
MaeIII GTNAC 1 cut(s) 156
MalI GATC 1 cut(s) 42
MbiI CCGCTC 1 cut(s) 114
MboI GATC 1 cut(s) 40
MboII GAAGA 1 cut(s) 28
MhlI GDGCHC 1 cut(s) 83
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 164
MmeI TCCRAC 2 cut(s) 144, 158
MnlI CCTC 1 cut(s) 208
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 101
MslI CAYNNNNRTG 1 cut(s) 245
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 1 cut(s) 78
MwoI GCNNNNNNNGC 2 cut(s) 111, 120
NciI CCSGG 1 cut(s) 78
NdeII GATC 1 cut(s) 40
NlaIII CATG 2 cut(s) 193, 212
NlaIV GGNNCC 4 cut(s) 81, 82, 130, 139
NmuCI GTSAC 1 cut(s) 156
PfeI GAWTC 1 cut(s) 257
PflMI CCANNNNNTGG 1 cut(s) 211
PkrI GCNGC 2 cut(s) 19, 116
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
PspN4I GGNNCC 4 cut(s) 81, 82, 130, 139
PspOMI GGGCCC 1 cut(s) 79
PspPI GGNCC 4 cut(s) 64, 79, 80, 128
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
RseI CAYNNNNRTG 1 cut(s) 245
SaqAI TTAA 1 cut(s) 101
SatI GCNGC 2 cut(s) 18, 115
Sau3AI GATC 1 cut(s) 40
Sau96I GGNCC 4 cut(s) 64, 79, 80, 128
SchI GAGTC 1 cut(s) 164
ScrFI CCNGG 1 cut(s) 78
SduI GDGCHC 1 cut(s) 83
SetI ASST 4 cut(s) 14, 55, 110, 230
SinI GGWCC 2 cut(s) 64, 128
SmiMI CAYNNNNRTG 1 cut(s) 245
SsiI CCGC 2 cut(s) 18, 114
SspMI CTAG 2 cut(s) 57, 225
StyD4I CCNGG 1 cut(s) 76
TaaI ACNGT 2 cut(s) 162, 278
TaqI TCGA 2 cut(s) 39, 49
TatI WGTACW 1 cut(s) 237
TauI GCSGC 2 cut(s) 20, 117
TfiI GAWTC 1 cut(s) 257
Tru1I TTAA 1 cut(s) 101
Tru9I TTAA 1 cut(s) 101
TscAI CASTG 2 cut(s) 124, 281
TseFI GTSAC 1 cut(s) 156
Tsp45I GTSAC 1 cut(s) 156
TspDTI ATGAA 1 cut(s) 270
TspRI CASTG 2 cut(s) 124, 281
Van91I CCANNNNNTGG 1 cut(s) 211
VpaK11BI GGWCC 2 cut(s) 64, 128
XspI CTAG 2 cut(s) 57, 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.