MD14G1129800.v1.1

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012210
Physical Location & Seq
Reverse (-)
20754102 .. 20755518
1417 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1129800.v1.1.491

Sequence Viewer

Length: 642 bp
ATGAGCAAGCCTCAGCGGCCACAGCGGCCCAAACCGCCGCCGTCCGGCCGTACGAACCTCGCCTCCTGCATCGTCGCCACCATCTTCCTAATTTTCGTCGTCATCGTCATCCTCATCGTCTACTTCACCGTCTTCAAGCCCAAAGACCCCAAAATCTCTGTCTCCGCCGTCCAGCTCCCTTCCTTCTCCATCCAAAACTCCACCGTCAACTTCACTTTCTCCCAATACGTCTCCGTCCACAACCCCAACCGCGCCGCCTTCAGCCACTACGACAGCACTCTCCAGCTTATCTACTCCGGCAGCCAAGTCGGGTTCATGTTCATACCCGCCGGCAAGATCGAAGCGGGTCAGACCCAGTTCATGGCCGCCACCTTCGCCGCCCAGTCGTTCCCGGTCTCCGATCCCAATCGGGCTTCCGGGATGGTCCAGAACCCACTGGGCCCCACTTTCGGAGATGGGTTGAGCGGTTTGAGGGGCACCGGCGCCGGAACCGTCGGTTCCACGATGCAGATTGAGTCGCGGTTGGAGATGGGGGGTCGGATTCGGGTCATGCACTTTCTTACCCACCATGTGGAGGCAAAATCTACGTGTGAAGTGTACATTGCTGTGAGTGATGGATCTGTGTTGGCTTTTCACTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

214

Amino Acids

23.01

Weight (kDa)

9.47

Isoelectric Point (pI)

36.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 476, 482
AccB7I CCANNNNNTGG 2 cut(s) 361, 571
AccBSI CCGCTC 1 cut(s) 465
AccI GTMKAC 1 cut(s) 120
AccII CGCG 2 cut(s) 252, 520
AclWI GGATC 2 cut(s) 395, 625
AcoI YGGCCR 3 cut(s) 17, 46, 363
AcuI CTGAAG 1 cut(s) 244
AcyI GRCGYC 1 cut(s) 483
AdeI CACNNNGTG 1 cut(s) 571
AfaI GTAC 2 cut(s) 52, 599
AfiI CCNNNNNNNGG 5 cut(s) 44, 361, 449, 571, 574
AflIII ACRYGT 1 cut(s) 587
AgsI TTSAA 1 cut(s) 136
AloI GAACNNNNNNTCC 2 cut(s) 47, 79
AluBI AGCT 2 cut(s) 175, 286
AluI AGCT 2 cut(s) 175, 286
Alw26I GTCTC 3 cut(s) 166, 235, 400
AlwI GGATC 2 cut(s) 395, 625
AoxI GGCC 5 cut(s) 17, 26, 46, 363, 439
ApaI GGGCCC 1 cut(s) 443
ApeKI GCWGC 1 cut(s) 300
ArsI GACNNNNNNTTYG 2 cut(s) 144, 176
AspLEI GCGC 2 cut(s) 254, 485
AspS9I GGNCC 4 cut(s) 27, 424, 439, 440
AsuC2I CCSGG 2 cut(s) 392, 418
AsuHPI GGTGA 1 cut(s) 118
AvaII GGWCC 1 cut(s) 424
BaeGI GKGCMC 2 cut(s) 443, 479
BanI GGYRCC 2 cut(s) 476, 482
BanII GRGCYC 1 cut(s) 443
BbsI GAAGAC 1 cut(s) 124
BbvCI CCTCAGC 1 cut(s) 12
BbvI GCAGC 1 cut(s) 312
BccI CCATC 6 cut(s) 89, 197, 415, 449, 523, 608
BceAI ACGGC 3 cut(s) 25, 33, 152
BcgI CGANNNNNNTGC 2 cut(s) 289, 323
BcnI CCSGG 2 cut(s) 392, 418
BcoDI GTCTC 3 cut(s) 166, 235, 400
BfoI RGCGCY 1 cut(s) 486
BglI GCCNNNNNGGC 2 cut(s) 16, 25
BisI GCNGC 7 cut(s) 17, 26, 38, 255, 301, 366, 378
BlsI GCNGC 7 cut(s) 18, 27, 39, 256, 302, 367, 379
Bme1390I CCNGG 2 cut(s) 392, 418
Bme18I GGWCC 1 cut(s) 424
BmgT120I GGNCC 4 cut(s) 27, 424, 439, 440
BmiI GGNNCC 6 cut(s) 441, 442, 478, 484, 490, 499
BmrFI CCNGG 2 cut(s) 392, 418
BmrI ACTGGG 3 cut(s) 349, 376, 446
BmsI GCATC 2 cut(s) 78, 495
BmuI ACTGGG 3 cut(s) 349, 376, 446
BpiI GAAGAC 1 cut(s) 124
BplI GAGNNNNNCTC 1 cut(s) 27
BpmI CTGGAG 1 cut(s) 266
Bpu10I CCTNAGC 1 cut(s) 12
BpuMI CCSGG 2 cut(s) 392, 418
BsaAI YACGTR 1 cut(s) 588
BsaBI GATNNNNATC 1 cut(s) 405
BsaHI GRCGYC 1 cut(s) 483
BsaI GGTCTC 1 cut(s) 400
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bsc4I CCNNNNNNNGG 5 cut(s) 44, 361, 449, 571, 574
Bse118I RCCGGY 2 cut(s) 329, 479
Bse1I ACTGG 3 cut(s) 355, 382, 441
Bse3DI GCAATG 1 cut(s) 600
Bse8I GATNNNNATC 1 cut(s) 405
BseGI GGATG 3 cut(s) 108, 189, 426
BseJI GATNNNNATC 1 cut(s) 405
BseLI CCNNNNNNNGG 5 cut(s) 44, 361, 449, 571, 574
BseMI GCAATG 1 cut(s) 600
BseMII CTCAG 1 cut(s) 26
BseNI ACTGG 3 cut(s) 355, 382, 441
BseSI GKGCMC 2 cut(s) 443, 479
BseX3I CGGCCG 1 cut(s) 46
BseXI GCAGC 1 cut(s) 312
Bsh1236I CGCG 2 cut(s) 252, 520
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 5 cut(s) 19, 28, 48, 365, 441
BshNI GGYRCC 2 cut(s) 476, 482
BsiEI CGRYCG 1 cut(s) 49
BsiSI CCGG 7 cut(s) 45, 297, 330, 392, 417, 480, 486
BsiWI CGTACG 1 cut(s) 50
BslI CCNNNNNNNGG 5 cut(s) 44, 361, 449, 571, 574
BsmAI GTCTC 3 cut(s) 166, 235, 400
BsmBI CGTCTC 1 cut(s) 235
BsnI GGCC 5 cut(s) 19, 28, 48, 365, 441
Bso31I GGTCTC 1 cut(s) 400
Bsp120I GGGCCC 1 cut(s) 439
Bsp1286I GDGCHC 2 cut(s) 443, 479
Bsp1407I TGTACA 1 cut(s) 597
Bsp143I GATC 3 cut(s) 336, 400, 617
BspANI GGCC 5 cut(s) 19, 28, 48, 365, 441
BspCNI CTCAG 1 cut(s) 25
BspFNI CGCG 2 cut(s) 252, 520
BspLI GGNNCC 6 cut(s) 441, 442, 478, 484, 490, 499
BspPI GGATC 2 cut(s) 395, 625
BspT107I GGYRCC 2 cut(s) 476, 482
BspTNI GGTCTC 1 cut(s) 400
BsrBI CCGCTC 1 cut(s) 465
BsrDI GCAATG 1 cut(s) 600
BsrFI RCCGGY 2 cut(s) 329, 479
BsrGI TGTACA 1 cut(s) 597
BsrI ACTGG 3 cut(s) 355, 382, 441
BssAI RCCGGY 2 cut(s) 329, 479
BssMI GATC 3 cut(s) 336, 400, 617
BssNI GRCGYC 1 cut(s) 483
Bst4CI ACNGT 4 cut(s) 130, 205, 493, 638
BstACI GRCGYC 1 cut(s) 483
BstAUI TGTACA 1 cut(s) 597
BstBAI YACGTR 1 cut(s) 588
BstC8I GCNNGC 2 cut(s) 8, 331
BstDEI CTNAG 1 cut(s) 12
BstF5I GGATG 3 cut(s) 108, 189, 426
BstFNI CGCG 2 cut(s) 252, 520
BstH2I RGCGCY 1 cut(s) 486
BstHHI GCGC 2 cut(s) 254, 485
BstKTI GATC 3 cut(s) 339, 403, 620
BstMAI GTCTC 3 cut(s) 166, 235, 400
BstMBI GATC 3 cut(s) 336, 400, 617
BstMCI CGRYCG 1 cut(s) 49
BstMWI GCNNNNNNNGC 5 cut(s) 16, 22, 25, 34, 374
BstSCI CCNGG 2 cut(s) 390, 416
BstSLI GKGCMC 2 cut(s) 443, 479
BstUI CGCG 2 cut(s) 252, 520
BstV1I GCAGC 1 cut(s) 312
BstV2I GAAGAC 1 cut(s) 124
BstX2I RGATCY 1 cut(s) 617
BstYI RGATCY 1 cut(s) 617
BstZI CGGCCG 1 cut(s) 46
BsuRI GGCC 5 cut(s) 19, 28, 48, 365, 441
BtsCI GGATG 3 cut(s) 108, 189, 426
BtsIMutI CAGTG 2 cut(s) 434, 634
Cac8I GCNNGC 2 cut(s) 8, 331
CfoI GCGC 2 cut(s) 254, 485
Cfr10I RCCGGY 2 cut(s) 329, 479
Cfr13I GGNCC 4 cut(s) 27, 424, 439, 440
Csp6I GTAC 2 cut(s) 51, 598
CviAII CATG 4 cut(s) 316, 361, 550, 569
CviQI GTAC 2 cut(s) 51, 598
DdeI CTNAG 1 cut(s) 12
DinI GGCGCC 1 cut(s) 484
DpnI GATC 3 cut(s) 338, 402, 619
DpnII GATC 3 cut(s) 336, 400, 617
DraIII CACNNNGTG 1 cut(s) 571
EaeI YGGCCR 3 cut(s) 17, 46, 363
EagI CGGCCG 1 cut(s) 46
EciI GGCGGA 1 cut(s) 154
EclXI CGGCCG 1 cut(s) 46
Eco24I GRGCYC 1 cut(s) 443
Eco31I GGTCTC 1 cut(s) 400
Eco47I GGWCC 1 cut(s) 424
Eco52I CGGCCG 1 cut(s) 46
Eco57I CTGAAG 1 cut(s) 244
EcoO109I RGGNCCY 1 cut(s) 440
EcoT38I GRGCYC 1 cut(s) 443
EgeI GGCGCC 1 cut(s) 484
EheI GGCGCC 1 cut(s) 484
Esp3I CGTCTC 1 cut(s) 235
FaeI CATG 4 cut(s) 319, 364, 553, 572
FaiI YATR 5 cut(s) 317, 323, 362, 551, 570
FatI CATG 4 cut(s) 315, 360, 549, 568
FauI CCCGC 2 cut(s) 334, 337
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 7 cut(s) 17, 26, 38, 255, 301, 366, 378
FokI GGATG 3 cut(s) 95, 176, 433
FriOI GRGCYC 1 cut(s) 443
Fsp4HI GCNGC 7 cut(s) 17, 26, 38, 255, 301, 366, 378
GlaI GCGC 2 cut(s) 253, 484
GluI GCNGC 7 cut(s) 17, 26, 38, 255, 301, 366, 378
GsuI CTGGAG 1 cut(s) 266
HaeII RGCGCY 1 cut(s) 486
HaeIII GGCC 5 cut(s) 19, 28, 48, 365, 441
HapII CCGG 7 cut(s) 45, 297, 330, 392, 417, 480, 486
HhaI GCGC 2 cut(s) 254, 485
Hin1I GRCGYC 1 cut(s) 483
Hin1II CATG 4 cut(s) 319, 364, 553, 572
Hin6I GCGC 2 cut(s) 252, 483
HinP1I GCGC 2 cut(s) 252, 483
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HinfI GANTC 2 cut(s) 515, 541
HpaII CCGG 7 cut(s) 45, 297, 330, 392, 417, 480, 486
HphI GGTGA 1 cut(s) 118
Hpy166II GTNNAC 4 cut(s) 121, 208, 238, 598
Hpy188I TCNGA 4 cut(s) 351, 400, 452, 540
Hpy188III TCNNGA 1 cut(s) 427
Hpy8I GTNNAC 4 cut(s) 121, 208, 238, 598
Hpy99I CGWCG 3 cut(s) 77, 101, 497
HpyAV CCTTC 4 cut(s) 189, 193, 268, 382
HpyCH4III ACNGT 4 cut(s) 130, 205, 493, 638
HpyCH4IV ACGT 2 cut(s) 228, 587
HpyCH4V TGCA 3 cut(s) 69, 508, 553
HpyF10VI GCNNNNNNNGC 5 cut(s) 16, 22, 25, 34, 374
HpyF3I CTNAG 1 cut(s) 12
HpySE526I ACGT 2 cut(s) 228, 587
Hsp92I GRCGYC 1 cut(s) 483
Hsp92II CATG 4 cut(s) 319, 364, 553, 572
HspAI GCGC 2 cut(s) 252, 483
KasI GGCGCC 1 cut(s) 482
KroI GCCGGC 1 cut(s) 329
KroNI GCCGGC 1 cut(s) 331
Kzo9I GATC 3 cut(s) 336, 400, 617
LmnI GCTCC 1 cut(s) 180
Lsp1109I GCAGC 1 cut(s) 312
LweI GCATC 2 cut(s) 78, 495
MaeII ACGT 2 cut(s) 228, 587
MalI GATC 3 cut(s) 338, 402, 619
MbiI CCGCTC 1 cut(s) 465
MboI GATC 3 cut(s) 336, 400, 617
MboII GAAGA 2 cut(s) 76, 124
MflI RGATCY 1 cut(s) 617
MhlI GDGCHC 2 cut(s) 443, 479
MluCI AATT 1 cut(s) 90
Mly113I GGCGCC 1 cut(s) 483
MlyI GAGTC 1 cut(s) 524
MmeI TCCRAC 2 cut(s) 504, 518
MnlI CCTC 6 cut(s) 21, 68, 73, 122, 465, 568
MroNI GCCGGC 1 cut(s) 329
MslI CAYNNNNRTG 1 cut(s) 605
MspA1I CMGCKG 2 cut(s) 16, 25
MspI CCGG 7 cut(s) 45, 297, 330, 392, 417, 480, 486
MspR9I CCNGG 2 cut(s) 392, 418
MvnI CGCG 2 cut(s) 252, 520
MwoI GCNNNNNNNGC 5 cut(s) 16, 22, 25, 34, 374
NaeI GCCGGC 1 cut(s) 331
NarI GGCGCC 1 cut(s) 483
NciI CCSGG 2 cut(s) 392, 418
NdeII GATC 3 cut(s) 336, 400, 617
NgoMIV GCCGGC 1 cut(s) 329
NlaIII CATG 4 cut(s) 319, 364, 553, 572
NlaIV GGNNCC 6 cut(s) 441, 442, 478, 484, 490, 499
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PdiI GCCGGC 1 cut(s) 331
PfeI GAWTC 1 cut(s) 541
Pfl23II CGTACG 1 cut(s) 50
PflMI CCANNNNNTGG 2 cut(s) 361, 571
PfoI TCCNGGA 1 cut(s) 416
PkrI GCNGC 7 cut(s) 18, 27, 39, 256, 302, 367, 379
PleI GAGTC 1 cut(s) 523
PluTI GGCGCC 1 cut(s) 486
PpsI GAGTC 1 cut(s) 523
Ppu21I YACGTR 1 cut(s) 588
PspLI CGTACG 1 cut(s) 50
PspN4I GGNNCC 6 cut(s) 441, 442, 478, 484, 490, 499
PspOMI GGGCCC 1 cut(s) 439
PspPI GGNCC 4 cut(s) 27, 424, 439, 440
PsuI RGATCY 1 cut(s) 617
RsaI GTAC 2 cut(s) 52, 599
RsaNI GTAC 2 cut(s) 51, 598
RseI CAYNNNNRTG 1 cut(s) 605
SatI GCNGC 7 cut(s) 17, 26, 38, 255, 301, 366, 378
Sau3AI GATC 3 cut(s) 336, 400, 617
Sau96I GGNCC 4 cut(s) 27, 424, 439, 440
SchI GAGTC 1 cut(s) 524
ScrFI CCNGG 2 cut(s) 392, 418
SduI GDGCHC 2 cut(s) 443, 479
SetI ASST 6 cut(s) 60, 177, 231, 288, 374, 590
SfaNI GCATC 2 cut(s) 78, 495
SfiI GGCCNNNNNGGCC 1 cut(s) 25
SfoI GGCGCC 1 cut(s) 484
SgrAI CRCCGGYG 1 cut(s) 479
SinI GGWCC 1 cut(s) 424
SmiMI CAYNNNNRTG 1 cut(s) 605
Sse9I AATT 1 cut(s) 90
SspDI GGCGCC 1 cut(s) 482
StyD4I CCNGG 2 cut(s) 390, 416
TaaI ACNGT 4 cut(s) 130, 205, 493, 638
TaiI ACGT 2 cut(s) 231, 590
TaqI TCGA 1 cut(s) 339
TasI AATT 1 cut(s) 90
TatI WGTACW 1 cut(s) 597
TauI GCSGC 6 cut(s) 19, 28, 40, 257, 368, 380
TfiI GAWTC 1 cut(s) 541
TscAI CASTG 2 cut(s) 441, 641
TseI GCWGC 1 cut(s) 300
TspDTI ATGAA 3 cut(s) 304, 310, 349
TspGWI ACGGA 1 cut(s) 223
TspRI CASTG 2 cut(s) 441, 641
Van91I CCANNNNNTGG 2 cut(s) 361, 571
VpaK11BI GGWCC 1 cut(s) 424
XmiI GTMKAC 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.