MD01G1078600.v1.1

Chitinase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012160
Physical Location & Seq
Reverse (-)
18543347 .. 18547082
3736 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1078600.v1.1.491

Sequence Viewer

Length: 1284 bp
ATGGCCAAGAAAAGAGAGCGCCGAGTCGCCCCGAGTGCGAAACGCGGCTACGATCGAGTCGAACCGCCGGACCAGTTCTGCGGCGCAATCCGGCAGCACACAATCACCGCATTTCTCTTCTTCTGCGTCGCTGTATCGACCGGCGTGCTCGCGTACCGCATCAAATATGCTCCGACGGCGAACCTGGCGGATTCCTACCCCGTCCACGAGCGAGGAATCGTGAAGCGTGATGTGAATTACCAAGAGATCCTCACTGAAAATGGAAAGGTTTCCGAAAATACGAGTAGGAGGCACTTTGAATATCCTGCATTGGCCTACATTACTCCATGGAATTCCAAGGGCTATGATATGGCAAAAAGGTTCAACTCCAAGTTTACTCATTTATCTCCAATTTGGTACGACTTAAAGAGCCAAGGCAATAGCTTAATTCTGGAAGGTAGGCATAATGCTGATACAGGATGGATCTCAGAGATTAGGATTGCAGGCGATGCTAGGGTATTGCCTAGAGTCGTTCTGGAAGCATTTCCAGCAGAGCTCCTTACAAAGAAGAAGCAGAGGAGTAAAGCTATTAATCTTCTAGTGAACGAGTGCAAGGAGATGGGATATGATGGTATTGTACTGGAATCTTGGTCAAGGTGGGCAGCGTTCAGAATTTTGCATGATCCAAGCATGCGGAATATGGCATTGCAGTTTATTAAAGAGCTTGGAGATGCACTACATGGTGTGAGCTTAGAGAAGAATGATAAGCAACGGTTGCAATTAGTCTATGTTATAGGTCCACCACAATCAGAGAAGCTCCAACCGCATGATTTTGGACCAAAAGATCTTCGGAGCTTGGATGATGCTGTGGATGGGTTCTCACTTATGACGTATGATTTCTCTGGGCCCCATAATCCAGGTCCCAATGCACCTTTGAAGTGGATTCATTCCACCCTCTGGCTGCTCCTTGGTACGGATGAAGATTGTGCTCGAGCTCGCAGAATATTTCTTGGCATCAATTTCTACGGCAATGATTTCCTCCTCAAAGGAGGTCCGGGTGGTGGTGCTATCACTGGAAGGGATTACCTGTCCTTGTTGGAGAAGCACAAGCCCCAGTTGCAATGGGAAAAGAACAGTGCAGAACATTTGTTCTATTACTCCGATGATGAAAATAACTACCACGCCGTGTTCTATCCGTCACCAATGTCAATTTCCGTGAGGCTAGAGGTAGGTCGCTACTGGGGATGCGGCATCTCGATATGGGAAATTGGGCAAGGTCTGGATTTCTTTTTCGATCTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

428

Amino Acids

48.67

Weight (kDa)

8.4

Isoelectric Point (pI)

46.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 179 - 418 5.4e-16 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012160)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01040 AT4G01040
fragaria_vesca FvH4_7g16200
malus_domestica MD01G1078600.v1.1 MD07G1147600.v1.1
prunus_persica Prupe.2G185600_v2.0.a1 Prupe.2G185600_v2.0.a1
pyrus_communis pycom01g10710
rosa_chinensis RchiOBHm_Chr1g0359211
rosa_laevigata RLG00000027893
rosa_multiflora Rmu_co8274979.1_g000001 Rmu_ssc0000135.1_g000019
rosa_roxburghii Rroxscaffold_4G00297250
rosa_rugosa Rorug01G0268400
rosa_samantha Rh1AG282300 Rh1BG248700 Rh1CG266500 Rh1DG277700
rosa_wichuraiana Rw1G025070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 936
AccII CGCG 2 cut(s) 45, 152
AciI CCGC 9 cut(s) 45, 65, 81, 108, 157, 188, 673, 803, 1227
AclWI GGATC 3 cut(s) 241, 470, 656
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 331, 651
AdeI CACNNNGTG 1 cut(s) 1165
AfaI GTAC 4 cut(s) 155, 398, 618, 952
AfiI CCNNNNNNNGG 3 cut(s) 936, 952, 1040
AgsI TTSAA 3 cut(s) 299, 364, 916
AjnI CCWGG 2 cut(s) 183, 895
AjuI GAANNNNNNNTTGG 2 cut(s) 1174, 1206
AluBI AGCT 8 cut(s) 423, 535, 566, 703, 729, 796, 834, 974
AluI AGCT 8 cut(s) 423, 535, 566, 703, 729, 796, 834, 974
Alw21I GWGCWC 4 cut(s) 150, 537, 970, 976
AlwI GGATC 3 cut(s) 241, 470, 656
Ama87I CYCGRG 2 cut(s) 31, 969
AoxI GGCC 3 cut(s) 3, 312, 884
ApaI GGGCCC 1 cut(s) 888
ApeKI GCWGC 3 cut(s) 94, 641, 940
ApoI RAATTY 2 cut(s) 331, 651
AseI ATTAAT 1 cut(s) 570
Asp700I GAANNNNTTC 2 cut(s) 268, 522
AspLEI GCGC 2 cut(s) 21, 86
AspS9I GGNCC 7 cut(s) 70, 776, 815, 884, 885, 899, 1031
AsuC2I CCSGG 1 cut(s) 1035
AsuHPI GGTGA 2 cut(s) 97, 1170
AvaI CYCGRG 2 cut(s) 31, 969
AvaII GGWCC 5 cut(s) 70, 776, 815, 899, 1031
BaeGI GKGCMC 1 cut(s) 888
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 3 cut(s) 537, 888, 976
BauI CACGAG 1 cut(s) 206
Bbv12I GWGCWC 4 cut(s) 150, 537, 970, 976
BbvI GCAGC 3 cut(s) 106, 653, 927
BccI CCATC 4 cut(s) 453, 592, 602, 845
BceAI ACGGC 3 cut(s) 192, 1021, 1148
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 2 cut(s) 185, 897
BcnI CCSGG 1 cut(s) 1035
BfaI CTAG 4 cut(s) 492, 504, 578, 1202
BfoI RGCGCY 1 cut(s) 22
BglII AGATCT 1 cut(s) 823
BisI GCNGC 6 cut(s) 46, 82, 95, 642, 941, 1228
BlsI GCNGC 6 cut(s) 47, 83, 96, 643, 942, 1229
Bme1390I CCNGG 3 cut(s) 185, 897, 1035
Bme18I GGWCC 5 cut(s) 70, 776, 815, 899, 1031
BmeT110I CYCGRG 2 cut(s) 31, 969
BmgT120I GGNCC 7 cut(s) 70, 776, 815, 884, 885, 899, 1031
BmiI GGNNCC 3 cut(s) 886, 887, 901
BmrFI CCNGG 3 cut(s) 185, 897, 1035
BmrI ACTGGG 2 cut(s) 1087, 1228
BmsI GCATC 7 cut(s) 168, 478, 700, 832, 1002, 1214, 1239
BmuI ACTGGG 2 cut(s) 1087, 1228
BpuMI CCSGG 1 cut(s) 1035
BsaJI CCNNGG 4 cut(s) 326, 336, 412, 946
Bsc4I CCNNNNNNNGG 3 cut(s) 936, 952, 1040
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 5 cut(s) 73, 624, 1057, 1093, 1223
Bse3DI GCAATG 3 cut(s) 683, 1015, 1106
BseBI CCWGG 2 cut(s) 185, 897
BseDI CCNNGG 4 cut(s) 326, 336, 412, 946
BseGI GGATG 5 cut(s) 464, 844, 856, 961, 1229
BseLI CCNNNNNNNGG 3 cut(s) 936, 952, 1040
BseMI GCAATG 3 cut(s) 683, 1015, 1106
BseMII CTCAG 1 cut(s) 480
BseNI ACTGG 5 cut(s) 73, 624, 1057, 1093, 1223
BseRI GAGGAG 2 cut(s) 571, 1010
BseSI GKGCMC 1 cut(s) 888
BseXI GCAGC 3 cut(s) 106, 653, 927
BsgI GTGCAG 1 cut(s) 1137
Bsh1236I CGCG 2 cut(s) 45, 152
Bsh1285I CGRYCG 2 cut(s) 55, 141
BshFI GGCC 3 cut(s) 5, 314, 886
BsiEI CGRYCG 2 cut(s) 55, 141
BsiHKAI GWGCWC 4 cut(s) 150, 537, 970, 976
BsiHKCI CYCGRG 2 cut(s) 31, 969
BsiSI CCGG 4 cut(s) 68, 91, 141, 1034
BslFI GGGAC 1 cut(s) 885
BslI CCNNNNNNNGG 3 cut(s) 936, 952, 1040
BsmFI GGGAC 1 cut(s) 885
BsnI GGCC 3 cut(s) 5, 314, 886
BsoBI CYCGRG 2 cut(s) 31, 969
Bsp120I GGGCCC 1 cut(s) 884
Bsp1286I GDGCHC 5 cut(s) 150, 537, 888, 970, 976
Bsp143I GATC 6 cut(s) 52, 246, 462, 661, 823, 1273
Bsp19I CCATGG 1 cut(s) 326
BspACI CCGC 9 cut(s) 45, 65, 81, 108, 157, 188, 673, 803, 1227
BspANI GGCC 3 cut(s) 5, 314, 886
BspCNI CTCAG 1 cut(s) 479
BspFNI CGCG 2 cut(s) 45, 152
BspLI GGNNCC 3 cut(s) 886, 887, 901
BspPI GGATC 3 cut(s) 241, 470, 656
BsrDI GCAATG 3 cut(s) 683, 1015, 1106
BsrFI RCCGGY 1 cut(s) 140
BsrI ACTGG 5 cut(s) 73, 624, 1057, 1093, 1223
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 4 cut(s) 326, 336, 412, 946
BssMI GATC 6 cut(s) 52, 246, 462, 661, 823, 1273
BssSI CACGAG 1 cut(s) 206
BssT1I CCWWGG 4 cut(s) 326, 336, 412, 946
Bst2BI CACGAG 1 cut(s) 206
Bst2UI CCWGG 2 cut(s) 185, 897
Bst4CI ACNGT 2 cut(s) 753, 1115
Bst6I CTCTTC 1 cut(s) 122
BstAPI GCANNNNNTGC 2 cut(s) 488, 754
BstC8I GCNNGC 5 cut(s) 146, 150, 484, 671, 976
BstDEI CTNAG 2 cut(s) 466, 730
BstDSI CCRYGG 1 cut(s) 326
BstF5I GGATG 5 cut(s) 464, 844, 856, 961, 1229
BstFNI CGCG 2 cut(s) 45, 152
BstH2I RGCGCY 1 cut(s) 22
BstHHI GCGC 2 cut(s) 21, 86
BstKTI GATC 6 cut(s) 55, 249, 465, 664, 826, 1276
BstMBI GATC 6 cut(s) 52, 246, 462, 661, 823, 1273
BstMCI CGRYCG 2 cut(s) 55, 141
BstMWI GCNNNNNNNGC 8 cut(s) 35, 176, 185, 488, 527, 754, 802, 1096
BstNI CCWGG 2 cut(s) 185, 897
BstNSI RCATGY 1 cut(s) 673
BstSCI CCNGG 3 cut(s) 183, 895, 1033
BstSLI GKGCMC 1 cut(s) 888
BstUI CGCG 2 cut(s) 45, 152
BstV1I GCAGC 3 cut(s) 106, 653, 927
BstX2I RGATCY 3 cut(s) 246, 462, 823
BstYI RGATCY 3 cut(s) 246, 462, 823
BsuRI GGCC 3 cut(s) 5, 314, 886
BtgI CCRYGG 1 cut(s) 326
BtgZI GCGATG 1 cut(s) 501
BtsCI GGATG 5 cut(s) 464, 844, 856, 961, 1229
BtsIMutI CAGTG 3 cut(s) 252, 1050, 1120
Cac8I GCNNGC 5 cut(s) 146, 150, 484, 671, 976
CfoI GCGC 2 cut(s) 21, 86
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 7 cut(s) 70, 776, 815, 884, 885, 899, 1031
CseI GACGC 1 cut(s) 115
Csp6I GTAC 4 cut(s) 154, 397, 617, 951
CviAII CATG 5 cut(s) 327, 659, 670, 719, 806
CviQI GTAC 4 cut(s) 154, 397, 617, 951
DdeI CTNAG 2 cut(s) 466, 730
DpnI GATC 6 cut(s) 54, 248, 464, 663, 825, 1275
DpnII GATC 6 cut(s) 52, 246, 462, 661, 823, 1273
DraIII CACNNNGTG 1 cut(s) 1165
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
EciI GGCGGA 1 cut(s) 203
Ecl136II GAGCTC 2 cut(s) 535, 974
Eco130I CCWWGG 4 cut(s) 326, 336, 412, 946
Eco24I GRGCYC 3 cut(s) 537, 888, 976
Eco47I GGWCC 5 cut(s) 70, 776, 815, 899, 1031
Eco53kI GAGCTC 2 cut(s) 535, 974
Eco88I CYCGRG 2 cut(s) 31, 969
EcoICRI GAGCTC 2 cut(s) 535, 974
EcoO109I RGGNCCY 2 cut(s) 885, 899
EcoRI GAATTC 1 cut(s) 331
EcoRII CCWGG 2 cut(s) 183, 895
EcoT14I CCWWGG 4 cut(s) 326, 336, 412, 946
EcoT38I GRGCYC 3 cut(s) 537, 888, 976
ErhI CCWWGG 4 cut(s) 326, 336, 412, 946
FaeI CATG 5 cut(s) 330, 662, 673, 722, 809
FaqI GGGAC 1 cut(s) 885
FatI CATG 5 cut(s) 326, 658, 669, 718, 805
Fnu4HI GCNGC 6 cut(s) 46, 82, 95, 642, 941, 1228
FokI GGATG 5 cut(s) 471, 851, 863, 968, 1236
FriOI GRGCYC 3 cut(s) 537, 888, 976
Fsp4HI GCNGC 6 cut(s) 46, 82, 95, 642, 941, 1228
FspBI CTAG 4 cut(s) 492, 504, 578, 1202
GlaI GCGC 2 cut(s) 20, 85
GluI GCNGC 6 cut(s) 46, 82, 95, 642, 941, 1228
HaeII RGCGCY 1 cut(s) 22
HaeIII GGCC 3 cut(s) 5, 314, 886
HapII CCGG 4 cut(s) 68, 91, 141, 1034
HgaI GACGC 1 cut(s) 115
HhaI GCGC 2 cut(s) 21, 86
Hin1II CATG 5 cut(s) 330, 662, 673, 722, 809
Hin6I GCGC 2 cut(s) 19, 84
HinP1I GCGC 2 cut(s) 19, 84
HinfI GANTC 7 cut(s) 24, 57, 191, 216, 507, 623, 922
HpaII CCGG 4 cut(s) 68, 91, 141, 1034
HphI GGTGA 2 cut(s) 97, 1170
Hpy166II GTNNAC 4 cut(s) 205, 375, 583, 779
Hpy188I TCNGA 7 cut(s) 174, 274, 469, 650, 790, 831, 1141
Hpy188III TCNNGA 5 cut(s) 220, 431, 515, 1234, 1259
Hpy8I GTNNAC 4 cut(s) 205, 375, 583, 779
Hpy99I CGWCG 2 cut(s) 131, 178
HpyAV CCTTC 2 cut(s) 428, 1050
HpyCH4III ACNGT 2 cut(s) 753, 1115
HpyCH4IV ACGT 1 cut(s) 869
HpyF10VI GCNNNNNNNGC 8 cut(s) 35, 176, 185, 488, 527, 754, 802, 1096
HpyF3I CTNAG 2 cut(s) 466, 730
HpySE526I ACGT 1 cut(s) 869
Hsp92II CATG 5 cut(s) 330, 662, 673, 722, 809
HspAI GCGC 2 cut(s) 19, 84
Kzo9I GATC 6 cut(s) 52, 246, 462, 661, 823, 1273
LmnI GCTCC 5 cut(s) 175, 540, 801, 831, 948
Lsp1109I GCAGC 3 cut(s) 106, 653, 927
LweI GCATC 7 cut(s) 168, 478, 700, 832, 1002, 1214, 1239
MaeI CTAG 4 cut(s) 492, 504, 578, 1202
MaeII ACGT 1 cut(s) 869
MaeIII GTNAC 1 cut(s) 1176
MalI GATC 6 cut(s) 54, 248, 464, 663, 825, 1275
MboI GATC 6 cut(s) 52, 246, 462, 661, 823, 1273
MboII GAAGA 8 cut(s) 109, 112, 559, 566, 748, 818, 971, 1268
MflI RGATCY 3 cut(s) 246, 462, 823
MhlI GDGCHC 5 cut(s) 150, 537, 888, 970, 976
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 9 cut(s) 235, 331, 390, 426, 651, 758, 997, 1188, 1245
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 33, 66, 516
MmeI TCCRAC 3 cut(s) 197, 823, 1056
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 2 cut(s) 268, 522
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 404, 425, 570, 696
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 4 cut(s) 68, 91, 141, 1034
MspR9I CCNGG 3 cut(s) 185, 897, 1035
MvaI CCWGG 2 cut(s) 185, 897
MvnI CGCG 2 cut(s) 45, 152
MwoI GCNNNNNNNGC 8 cut(s) 35, 176, 185, 488, 527, 754, 802, 1096
NciI CCSGG 1 cut(s) 1035
NcoI CCATGG 1 cut(s) 326
NdeII GATC 6 cut(s) 52, 246, 462, 661, 823, 1273
NlaIII CATG 5 cut(s) 330, 662, 673, 722, 809
NlaIV GGNNCC 3 cut(s) 886, 887, 901
NmeAIII GCCGAG 1 cut(s) 47
NmuCI GTSAC 1 cut(s) 1176
NspI RCATGY 1 cut(s) 673
PaeI GCATGC 1 cut(s) 673
PaeR7I CTCGAG 1 cut(s) 969
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PdmI GAANNNNTTC 2 cut(s) 268, 522
PfeI GAWTC 4 cut(s) 191, 216, 623, 922
PflMI CCANNNNNTGG 1 cut(s) 936
PkrI GCNGC 6 cut(s) 47, 83, 96, 643, 942, 1229
Ple19I CGATCG 1 cut(s) 55
PleI GAGTC 3 cut(s) 32, 65, 515
PpsI GAGTC 3 cut(s) 32, 65, 515
PpuMI RGGWCCY 1 cut(s) 899
PshBI ATTAAT 1 cut(s) 570
Psp124BI GAGCTC 2 cut(s) 537, 976
Psp5II RGGWCCY 1 cut(s) 899
Psp6I CCWGG 2 cut(s) 183, 895
PspGI CCWGG 2 cut(s) 183, 895
PspN4I GGNNCC 3 cut(s) 886, 887, 901
PspOMI GGGCCC 1 cut(s) 884
PspPI GGNCC 7 cut(s) 70, 776, 815, 884, 885, 899, 1031
PspPPI RGGWCCY 1 cut(s) 899
PspXI VCTCGAGB 1 cut(s) 969
PsuI RGATCY 3 cut(s) 246, 462, 823
PvuI CGATCG 1 cut(s) 55
RsaI GTAC 4 cut(s) 155, 398, 618, 952
RsaNI GTAC 4 cut(s) 154, 397, 617, 951
SacI GAGCTC 2 cut(s) 537, 976
SaqAI TTAA 4 cut(s) 404, 425, 570, 696
SatI GCNGC 6 cut(s) 46, 82, 95, 642, 941, 1228
Sau3AI GATC 6 cut(s) 52, 246, 462, 661, 823, 1273
Sau96I GGNCC 7 cut(s) 70, 776, 815, 884, 885, 899, 1031
SchI GAGTC 3 cut(s) 33, 66, 516
ScrFI CCNGG 3 cut(s) 185, 897, 1035
SduI GDGCHC 5 cut(s) 150, 537, 888, 970, 976
SfaNI GCATC 7 cut(s) 168, 478, 700, 832, 1002, 1214, 1239
Sfr274I CTCGAG 1 cut(s) 969
SinI GGWCC 5 cut(s) 70, 776, 815, 899, 1031
SlaI CTCGAG 1 cut(s) 969
SmlI CTYRAG 1 cut(s) 969
SmoI CTYRAG 1 cut(s) 969
SphI GCATGC 1 cut(s) 673
Sse9I AATT 9 cut(s) 235, 331, 390, 426, 651, 758, 997, 1188, 1245
SsiI CCGC 9 cut(s) 45, 65, 81, 108, 157, 188, 673, 803, 1227
SspI AATATT 1 cut(s) 984
SspMI CTAG 4 cut(s) 492, 504, 578, 1202
SstI GAGCTC 2 cut(s) 537, 976
StyD4I CCNGG 3 cut(s) 183, 895, 1033
StyI CCWWGG 4 cut(s) 326, 336, 412, 946
TaaI ACNGT 2 cut(s) 753, 1115
TaiI ACGT 1 cut(s) 872
TaqI TCGA 6 cut(s) 55, 60, 137, 970, 1235, 1272
TasI AATT 9 cut(s) 235, 331, 390, 426, 651, 758, 997, 1188, 1245
TatI WGTACW 1 cut(s) 616
TauI GCSGC 3 cut(s) 48, 84, 1230
TfiI GAWTC 4 cut(s) 191, 216, 623, 922
Tru1I TTAA 4 cut(s) 404, 425, 570, 696
Tru9I TTAA 4 cut(s) 404, 425, 570, 696
TscAI CASTG 3 cut(s) 259, 1057, 1120
TseFI GTSAC 1 cut(s) 1176
TseI GCWGC 3 cut(s) 94, 641, 940
Tsp45I GTSAC 1 cut(s) 1176
TspDTI ATGAA 3 cut(s) 914, 972, 1161
TspGWI ACGGA 3 cut(s) 968, 1164, 1183
TspRI CASTG 3 cut(s) 259, 1057, 1120
Van91I CCANNNNNTGG 1 cut(s) 936
VpaK11BI GGWCC 5 cut(s) 70, 776, 815, 899, 1031
VspI ATTAAT 1 cut(s) 570
XapI RAATTY 2 cut(s) 331, 651
XceI RCATGY 1 cut(s) 673
XhoI CTCGAG 1 cut(s) 969
XmnI GAANNNNTTC 2 cut(s) 268, 522
XspI CTAG 4 cut(s) 492, 504, 578, 1202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.