RLG00000027893

Chitinase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0012160
Physical Location & Seq
Forward (+)
16424535 .. 16427889
3355 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027893

Sequence Viewer

Length: 1299 bp
ATGGCGAAGAAAAAAGACCGCCGAGTCGTCGCGCCGAGTTCCGATCGCCGCAAAGACCGAGTCGAACCGTCACACCAACTCGCCCACTCAGCCTCATCCTCCTCAAATCGGAAGCTCGTAATCACCTTCGTCCTCTTCTTCGTCGTATCTTCAGCAATCGCGGTACTCGTGTACCGCCTCAATTATGCTCCGATCACAGACCGGACGGAGTCCTACGTGTTCCGGCGAGGCCTCGTTAAGCCCGACGTGACCTACCAAGAGATCCTCACTGAAAATGCAGTTGTTTCGGAAAATACGACTGGCAGGCACTATACTTATCCTGTATTAGCTTACATTACTCCATGGAATTCTAAGGGCTATGACTTGGCAAAAAGGTTTAACTCCAAATTTACACATATATCTCCAGTCTGGTATGATCTGAAGAGTCAAGGGACCAGCTTAATTCTGGAAGGCAGACATAATACTGATATTGGGTGGATCTCAGACCTTAGAATAGCAGGAGACGCTTGGGTATTGCCTAGAATTGTTTTGGAAGCTTTTCCAGCAGAGCTGCTTACAAAGAAAAAGCAGAGGAATAAGGCTATTGATCTTATAGTAAGCGAGTGCAAGGAAATGGGCTATGATGGAATTGTGCTGGAATCTTGGTCAAGGTGGGCAGCTTACCGTGTTTTGCATGATCCAAAGATGCGGAATTTGGCATTACAGTTTATAAAAGATCTTGGAGATGCACTGCATGCTGTGACTTCAGAGAGGAACAATAAGCACCGCTTGCAGTTGGTGTATGTTATAGGCCCACCACATTCAGAGAAGCTCCAAGTGCATGATTTTGGACCTAAAGATCTTCATAGCTTGAGTGATGCTGTGGATGGATTCTCACTTATGACGTATGACTTCTCAGGGCCTCATAATCCAGGTCCCAATGCACCTTTGAAGTGGATCGATTCCACCCTCCAGCTGCTTCTTGGTACCAATAAAAACAGTGCTCTGGCCAGCAAGATACTTCTCGGCATCAATTTCTATGGAAATGATTACATCCTCTCAGGAGGTCCTGGTGGTGGTGCCATCACCGGAAGAGATTACATCTCCTTGTTGGAGAAGCACAAGCCTGCATTTCGATGGGAAAAGAACAGCGCAGAACATTTGTTCTTGTACACTGATAATAATCATAACAATCACGCTGTGTTCTACCCGTCGTTACTATCAATTTCCATGCGGCTAGAGGAAGCTCGTAAATGGGGTTGTGGCATCTCGATATGGGAAATTGGGCAAGGTCTGGATTACTTTTACAATCTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

433

Amino Acids

48.94

Weight (kDa)

9.15

Isoelectric Point (pI)

44.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 184 - 423 3.1e-17 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012160)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01040 AT4G01040
fragaria_vesca FvH4_7g16200
malus_domestica MD01G1078600.v1.1 MD07G1147600.v1.1
prunus_persica Prupe.2G185600_v2.0.a1 Prupe.2G185600_v2.0.a1
pyrus_communis pycom01g10710
rosa_chinensis RchiOBHm_Chr1g0359211
rosa_laevigata RLG00000027893
rosa_multiflora Rmu_co8274979.1_g000001 Rmu_ssc0000135.1_g000019
rosa_roxburghii Rroxscaffold_4G00297250
rosa_rugosa Rorug01G0268400
rosa_samantha Rh1AG282300 Rh1BG248700 Rh1CG266500 Rh1DG277700
rosa_wichuraiana Rw1G025070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 710
AasI GACNNNNNNGTC 1 cut(s) 23
Acc65I GGTACC 1 cut(s) 965
AccB1I GGYRCC 2 cut(s) 965, 1058
AccII CGCG 2 cut(s) 32, 161
AciI CCGC 7 cut(s) 19, 49, 161, 175, 688, 766, 1213
AclWI GGATC 4 cut(s) 256, 485, 671, 944
AcoI YGGCCR 1 cut(s) 987
AcsI RAATTY 3 cut(s) 346, 386, 691
AcuI CTGAAG 3 cut(s) 135, 440, 729
AdeI CACNNNGTG 1 cut(s) 1180
AfaI GTAC 4 cut(s) 165, 173, 967, 1151
AfiI CCNNNNNNNGG 2 cut(s) 108, 1055
AflIII ACRYGT 1 cut(s) 216
AgsI TTSAA 1 cut(s) 931
AjiI CACGTC 1 cut(s) 247
AjnI CCWGG 2 cut(s) 910, 1048
Alw21I GWGCWC 1 cut(s) 985
Alw26I GTCTC 1 cut(s) 495
AlwI GGATC 4 cut(s) 256, 485, 671, 944
AoxI GGCC 4 cut(s) 229, 790, 899, 987
ApeKI GCWGC 3 cut(s) 550, 656, 955
ApoI RAATTY 3 cut(s) 346, 386, 691
ArsI GACNNNNNNTTYG 2 cut(s) 45, 77
Asp700I GAANNNNTTC 1 cut(s) 537
Asp718I GGTACC 1 cut(s) 965
AspLEI GCGC 2 cut(s) 34, 1133
AspS9I GGNCC 6 cut(s) 432, 791, 830, 899, 914, 1046
AsuHPI GGTGA 2 cut(s) 115, 1057
AvaII GGWCC 4 cut(s) 432, 830, 914, 1046
BaeI ACNNNNGTAYC 4 cut(s) 155, 155, 188, 188
BalI TGGCCA 1 cut(s) 989
BanI GGYRCC 2 cut(s) 965, 1058
BauI CACGAG 1 cut(s) 167
Bbv12I GWGCWC 1 cut(s) 985
BbvI GCAGC 3 cut(s) 537, 668, 942
BccI CCATC 4 cut(s) 617, 860, 1070, 1110
BcgI CGANNNNNNTGC 2 cut(s) 267, 301
BciT130I CCWGG 2 cut(s) 912, 1050
BcoDI GTCTC 1 cut(s) 495
BfaI CTAG 2 cut(s) 519, 1217
BglII AGATCT 2 cut(s) 715, 838
BisI GCNGC 5 cut(s) 49, 551, 657, 956, 1214
BlsI GCNGC 5 cut(s) 50, 552, 658, 957, 1215
Bme1390I CCNGG 2 cut(s) 912, 1050
Bme18I GGWCC 4 cut(s) 432, 830, 914, 1046
BmgBI CACGTC 1 cut(s) 247
BmgT120I GGNCC 6 cut(s) 432, 791, 830, 899, 914, 1046
BmiI GGNNCC 4 cut(s) 433, 916, 967, 1060
BmrFI CCNGG 2 cut(s) 912, 1050
BmsI GCATC 5 cut(s) 675, 715, 847, 1017, 1254
BpmI CTGGAG 2 cut(s) 387, 935
BpuEI CTTGAG 1 cut(s) 871
Bsa29I ATCGAT 1 cut(s) 939
BsaAI YACGTR 1 cut(s) 217
BsaBI GATNNNNATC 1 cut(s) 1161
BsaJI CCNNGG 1 cut(s) 341
BsaWI WCCGGW 2 cut(s) 201, 1067
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 1055
Bse1I ACTGG 2 cut(s) 304, 404
Bse8I GATNNNNATC 1 cut(s) 1161
BseBI CCWGG 2 cut(s) 912, 1050
BseCI ATCGAT 1 cut(s) 939
BseDI CCNNGG 1 cut(s) 341
BseGI GGATG 3 cut(s) 95, 871, 1032
BseJI GATNNNNATC 1 cut(s) 1161
BseLI CCNNNNNNNGG 2 cut(s) 108, 1055
BseMII CTCAG 4 cut(s) 102, 495, 909, 1053
BseNI ACTGG 2 cut(s) 304, 404
BseRI GAGGAG 1 cut(s) 91
BseXI GCAGC 3 cut(s) 537, 668, 942
Bsh1236I CGCG 2 cut(s) 32, 161
Bsh1285I CGRYCG 1 cut(s) 46
BshFI GGCC 4 cut(s) 231, 792, 901, 989
BshNI GGYRCC 2 cut(s) 965, 1058
BshVI ATCGAT 1 cut(s) 939
BsiEI CGRYCG 1 cut(s) 46
BsiHKAI GWGCWC 1 cut(s) 985
BsiSI CCGG 3 cut(s) 202, 223, 1068
BslFI GGGAC 2 cut(s) 445, 900
BslI CCNNNNNNNGG 2 cut(s) 108, 1055
BsmAI GTCTC 1 cut(s) 495
BsmBI CGTCTC 1 cut(s) 495
BsmFI GGGAC 2 cut(s) 445, 900
BsnI GGCC 4 cut(s) 231, 792, 901, 989
Bsp1286I GDGCHC 1 cut(s) 985
Bsp1407I TGTACA 1 cut(s) 1149
Bsp19I CCATGG 1 cut(s) 341
BspACI CCGC 7 cut(s) 19, 49, 161, 175, 688, 766, 1213
BspANI GGCC 4 cut(s) 231, 792, 901, 989
BspCNI CTCAG 4 cut(s) 101, 494, 908, 1052
BspDI ATCGAT 1 cut(s) 939
BspFNI CGCG 2 cut(s) 32, 161
BspLI GGNNCC 4 cut(s) 433, 916, 967, 1060
BspPI GGATC 4 cut(s) 256, 485, 671, 944
BspT107I GGYRCC 2 cut(s) 965, 1058
BsrGI TGTACA 1 cut(s) 1149
BsrI ACTGG 2 cut(s) 304, 404
BssECI CCNNGG 1 cut(s) 341
BssSI CACGAG 1 cut(s) 167
BssT1I CCWWGG 1 cut(s) 341
Bst2BI CACGAG 1 cut(s) 167
Bst2UI CCWGG 2 cut(s) 912, 1050
Bst4CI ACNGT 4 cut(s) 69, 665, 705, 980
Bst6I CTCTTC 3 cut(s) 140, 416, 1066
BstAPI GCANNNNNTGC 2 cut(s) 734, 769
BstAUI TGTACA 1 cut(s) 1149
BstBAI YACGTR 1 cut(s) 217
BstC8I GCNNGC 5 cut(s) 305, 735, 770, 991, 1107
BstDEI CTNAG 6 cut(s) 88, 351, 481, 488, 895, 1039
BstDSI CCRYGG 1 cut(s) 341
BstF5I GGATG 3 cut(s) 95, 871, 1032
BstFNI CGCG 2 cut(s) 32, 161
BstHHI GCGC 2 cut(s) 34, 1133
BstMAI GTCTC 1 cut(s) 495
BstMCI CGRYCG 1 cut(s) 46
BstMWI GCNNNNNNNGC 6 cut(s) 89, 503, 542, 734, 769, 817
BstNI CCWGG 2 cut(s) 912, 1050
BstNSI RCATGY 1 cut(s) 737
BstSCI CCNGG 2 cut(s) 910, 1048
BstUI CGCG 2 cut(s) 32, 161
BstV1I GCAGC 3 cut(s) 537, 668, 942
BstX2I RGATCY 4 cut(s) 261, 477, 715, 838
BstYI RGATCY 4 cut(s) 261, 477, 715, 838
Bsu15I ATCGAT 1 cut(s) 939
BsuRI GGCC 4 cut(s) 231, 792, 901, 989
BsuTUI ATCGAT 1 cut(s) 939
BtgI CCRYGG 1 cut(s) 341
BtrI CACGTC 1 cut(s) 247
BtsCI GGATG 3 cut(s) 95, 871, 1032
BtsI GCAGTG 1 cut(s) 728
BtsIMutI CAGTG 4 cut(s) 267, 728, 985, 1152
Cac8I GCNNGC 5 cut(s) 305, 735, 770, 991, 1107
CfoI GCGC 2 cut(s) 34, 1133
Cfr13I GGNCC 6 cut(s) 432, 791, 830, 899, 914, 1046
ClaI ATCGAT 1 cut(s) 939
CseI GACGC 1 cut(s) 512
Csp6I GTAC 4 cut(s) 164, 172, 966, 1150
CviAII CATG 5 cut(s) 342, 674, 734, 821, 1210
CviQI GTAC 4 cut(s) 164, 172, 966, 1150
DdeI CTNAG 6 cut(s) 88, 351, 481, 488, 895, 1039
DraIII CACNNNGTG 1 cut(s) 1180
DrdI GACNNNNNNGTC 1 cut(s) 23
DseDI GACNNNNNNGTC 1 cut(s) 23
EaeI YGGCCR 1 cut(s) 987
Eam1104I CTCTTC 3 cut(s) 140, 416, 1066
EarI CTCTTC 3 cut(s) 140, 416, 1066
Eco130I CCWWGG 1 cut(s) 341
Eco147I AGGCCT 1 cut(s) 231
Eco47I GGWCC 4 cut(s) 432, 830, 914, 1046
Eco57I CTGAAG 3 cut(s) 135, 440, 729
EcoO109I RGGNCCY 3 cut(s) 899, 914, 1046
EcoRI GAATTC 1 cut(s) 346
EcoRII CCWGG 2 cut(s) 910, 1048
EcoT14I CCWWGG 1 cut(s) 341
ErhI CCWWGG 1 cut(s) 341
Esp3I CGTCTC 1 cut(s) 495
FaeI CATG 5 cut(s) 345, 677, 737, 824, 1213
FalI AAGNNNNNCTT 2 cut(s) 752, 784
FaqI GGGAC 2 cut(s) 445, 900
FatI CATG 5 cut(s) 341, 673, 733, 820, 1209
Fnu4HI GCNGC 5 cut(s) 49, 551, 657, 956, 1214
FokI GGATG 3 cut(s) 82, 878, 1019
Fsp4HI GCNGC 5 cut(s) 49, 551, 657, 956, 1214
FspBI CTAG 2 cut(s) 519, 1217
GlaI GCGC 2 cut(s) 33, 1132
GluI GCNGC 5 cut(s) 49, 551, 657, 956, 1214
GsuI CTGGAG 2 cut(s) 387, 935
HaeIII GGCC 4 cut(s) 231, 792, 901, 989
HapII CCGG 3 cut(s) 202, 223, 1068
HgaI GACGC 1 cut(s) 512
HhaI GCGC 2 cut(s) 34, 1133
Hin1II CATG 5 cut(s) 345, 677, 737, 824, 1213
Hin6I GCGC 2 cut(s) 32, 1131
HinP1I GCGC 2 cut(s) 32, 1131
HindIII AAGCTT 1 cut(s) 534
HinfI GANTC 7 cut(s) 24, 60, 209, 424, 638, 870, 941
HpaII CCGG 3 cut(s) 202, 223, 1068
HphI GGTGA 2 cut(s) 115, 1057
Hpy166II GTNNAC 2 cut(s) 172, 1152
Hpy188I TCNGA 8 cut(s) 43, 111, 192, 289, 420, 484, 748, 805
Hpy188III TCNNGA 4 cut(s) 446, 1041, 1249, 1274
Hpy8I GTNNAC 2 cut(s) 172, 1152
Hpy99I CGWCG 4 cut(s) 32, 146, 248, 1195
HpyAV CCTTC 2 cut(s) 136, 443
HpyCH4III ACNGT 4 cut(s) 69, 665, 705, 980
HpyCH4IV ACGT 3 cut(s) 216, 246, 884
HpyCH4V TGCA 9 cut(s) 278, 606, 673, 728, 733, 772, 820, 923, 1109
HpyF10VI GCNNNNNNNGC 6 cut(s) 89, 503, 542, 734, 769, 817
HpyF3I CTNAG 6 cut(s) 88, 351, 481, 488, 895, 1039
HpySE526I ACGT 3 cut(s) 216, 246, 884
Hsp92II CATG 5 cut(s) 345, 677, 737, 824, 1213
HspAI GCGC 2 cut(s) 32, 1131
KpnI GGTACC 1 cut(s) 969
LmnI GCTCC 2 cut(s) 193, 816
Lsp1109I GCAGC 3 cut(s) 537, 668, 942
LweI GCATC 5 cut(s) 675, 715, 847, 1017, 1254
MaeI CTAG 2 cut(s) 519, 1217
MaeII ACGT 3 cut(s) 216, 246, 884
MaeIII GTNAC 4 cut(s) 69, 247, 739, 1194
MboII GAAGA 8 cut(s) 19, 127, 130, 141, 433, 833, 1083, 1283
MflI RGATCY 4 cut(s) 261, 477, 715, 838
MhlI GDGCHC 1 cut(s) 985
MlsI TGGCCA 1 cut(s) 989
MluNI TGGCCA 1 cut(s) 989
MlyI GAGTC 4 cut(s) 33, 69, 218, 433
MmeI TCCRAC 1 cut(s) 1071
Mox20I TGGCCA 1 cut(s) 989
MroXI GAANNNNTTC 1 cut(s) 537
MscI TGGCCA 1 cut(s) 989
MseI TTAA 3 cut(s) 237, 378, 440
MslI CAYNNNNRTG 1 cut(s) 1114
Msp20I TGGCCA 1 cut(s) 989
MspA1I CMGCKG 1 cut(s) 955
MspI CCGG 3 cut(s) 202, 223, 1068
MspR9I CCNGG 2 cut(s) 912, 1050
MvaI CCWGG 2 cut(s) 912, 1050
MvnI CGCG 2 cut(s) 32, 161
MwoI GCNNNNNNNGC 6 cut(s) 89, 503, 542, 734, 769, 817
NcoI CCATGG 1 cut(s) 341
NlaIII CATG 5 cut(s) 345, 677, 737, 824, 1213
NlaIV GGNNCC 4 cut(s) 433, 916, 967, 1060
NmeAIII GCCGAG 3 cut(s) 47, 60, 984
NmuCI GTSAC 3 cut(s) 69, 247, 739
NspI RCATGY 1 cut(s) 737
PaeI GCATGC 1 cut(s) 737
PceI AGGCCT 1 cut(s) 231
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 240, 293
PdmI GAANNNNTTC 1 cut(s) 537
PfeI GAWTC 3 cut(s) 638, 870, 941
PflFI GACNNNGTC 2 cut(s) 59, 208
PkrI GCNGC 5 cut(s) 50, 552, 658, 957, 1215
Ple19I CGATCG 1 cut(s) 46
PleI GAGTC 4 cut(s) 32, 68, 217, 432
PpsI GAGTC 4 cut(s) 32, 68, 217, 432
Ppu21I YACGTR 1 cut(s) 217
PpuMI RGGWCCY 2 cut(s) 914, 1046
PsiI TTATAA 1 cut(s) 710
Psp5II RGGWCCY 2 cut(s) 914, 1046
Psp6I CCWGG 2 cut(s) 910, 1048
PspGI CCWGG 2 cut(s) 910, 1048
PspN4I GGNNCC 4 cut(s) 433, 916, 967, 1060
PspPI GGNCC 6 cut(s) 432, 791, 830, 899, 914, 1046
PspPPI RGGWCCY 2 cut(s) 914, 1046
PsuI RGATCY 4 cut(s) 261, 477, 715, 838
PsyI GACNNNGTC 2 cut(s) 59, 208
PvuI CGATCG 1 cut(s) 46
PvuII CAGCTG 1 cut(s) 955
RsaI GTAC 4 cut(s) 165, 173, 967, 1151
RsaNI GTAC 4 cut(s) 164, 172, 966, 1150
RseI CAYNNNNRTG 1 cut(s) 1114
SaqAI TTAA 3 cut(s) 237, 378, 440
SatI GCNGC 5 cut(s) 49, 551, 657, 956, 1214
Sau96I GGNCC 6 cut(s) 432, 791, 830, 899, 914, 1046
SchI GAGTC 4 cut(s) 33, 69, 218, 433
ScrFI CCNGG 2 cut(s) 912, 1050
SduI GDGCHC 1 cut(s) 985
SfaNI GCATC 5 cut(s) 675, 715, 847, 1017, 1254
SinI GGWCC 4 cut(s) 432, 830, 914, 1046
SmiMI CAYNNNNRTG 1 cut(s) 1114
SmlI CTYRAG 1 cut(s) 850
SmoI CTYRAG 1 cut(s) 850
SphI GCATGC 1 cut(s) 737
SseBI AGGCCT 1 cut(s) 231
SsiI CCGC 7 cut(s) 19, 49, 161, 175, 688, 766, 1213
SspMI CTAG 2 cut(s) 519, 1217
StuI AGGCCT 1 cut(s) 231
StyD4I CCNGG 2 cut(s) 910, 1048
StyI CCWWGG 1 cut(s) 341
TaaI ACNGT 4 cut(s) 69, 665, 705, 980
TaiI ACGT 3 cut(s) 219, 249, 887
TaqI TCGA 4 cut(s) 63, 939, 1114, 1250
TaqII GACCGA 1 cut(s) 72
TatI WGTACW 1 cut(s) 1149
TauI GCSGC 2 cut(s) 51, 1216
TfiI GAWTC 3 cut(s) 638, 870, 941
Tru1I TTAA 3 cut(s) 237, 378, 440
Tru9I TTAA 3 cut(s) 237, 378, 440
TscAI CASTG 4 cut(s) 274, 735, 985, 1159
TseFI GTSAC 3 cut(s) 69, 247, 739
TseI GCWGC 3 cut(s) 550, 656, 955
Tsp45I GTSAC 3 cut(s) 69, 247, 739
TspDTI ATGAA 1 cut(s) 833
TspGWI ACGGA 1 cut(s) 221
TspRI CASTG 4 cut(s) 274, 735, 985, 1159
Tth111I GACNNNGTC 2 cut(s) 59, 208
VpaK11BI GGWCC 4 cut(s) 432, 830, 914, 1046
XapI RAATTY 3 cut(s) 346, 386, 691
XceI RCATGY 1 cut(s) 737
XcmI CCANNNNNNNNNTGG 2 cut(s) 442, 959
XmnI GAANNNNTTC 1 cut(s) 537
XspI CTAG 2 cut(s) 519, 1217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.