Rh1DG277700

Chitinase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0012160
Physical Location & Seq
Reverse (-)
49912542 .. 49924453
11912 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG277700.1

Sequence Viewer

Length: 1212 bp
ATGAAAAATTCGGCCAGTGACGGCGCCAATGGCGAAGAAAAAAGACCGCCGAGTTCCGATCGCCGCAAAGACCGAGTCGAACCGTCGCACCAACTCGCCCACTCAGCCTCATCCTCCTCAAATCGGAAGCTCGTAATCACCTTCGTCCTCTTCTTCGTCGTATCTTCAGCAATCTCAGTACTCGTGTACCGCCTCAATTATGCTCCGATCACAGACCGGACGGAGTCCTACGTGTTCCGGCGAGGCCTCGTTAAGCCCGACGTGACCTACCAAGAGATCCTCACTGAAAATGCTGTTGTTTCGGAAAATACGACTGGCAGGCACTATACTTATCCTGTATTAGCTTACATTACTCCATGGAATTCTAAGGGCTATGACTTGGCAAAAAGGTTTAACTCCAAATTTACACATATATCTCCAGTCTGGTATGATCTGAAGAGTCAAGGGACCAGCTTAATTCTGGAAGGCAGACATAATGCTGATATTGGGTGGATCTCAGACCTTAGAATAGCAGGAGATGCTTGGGTATTGCCTAGAATTGTTCTGGAAGCTTTTCCAGCAGAGCTGCTTACAAAGAAAAAGCAGAGGAATAAGGCTATTGATCTTATAGCACTACAGTTTATAAAAGATCTTGGAGATGCACTGCATGCTGTGACTTCAGAGAGGAACAATAAGCACCGCTTGCAGTTGGTGTATGTTATAGGCCCACCACATTCAGAGAAGCTCCAAGTGCATGATTTTGGACCTAAAGATCTTCATAGGTTGAGTGATGCTGTGGATGGATTCTCACTTATGACGTATGACTTCTCAGGGCCTCATAATCCAGGTCCCAATGCACCTCTGAAGTGGATCGATTCCACCCTCCAGCTGCTTCTTGGTACCAATAAAAACAGTGCTCTGGCCAGCAAGATACTTCTCGGCATCAATTTCTATGGAAATGATTACATCCTCTCAGGAGGTCCTGGTGGTGGTGCTATCACCGGAAGAGATTACATCTCCTTGTTGCAGAAGCACAAGCCTGCATTTCGATGGGAAAAGAACAGCGCAGAACATTTGTTCTTCTACACTGATAATGATCATAACAATCATGCTGTGTTCTACCCATCGTTACTGTCAATTTCCATGCGGCTAGAGGAAGCTCGTAAATGGGGTTGTGGCATGTCGATATGGGAAATTGGGCAAGGTCTGGATTACTTTTACGATCTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

403

Amino Acids

45.41

Weight (kDa)

8.82

Isoelectric Point (pI)

46.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 206 - 394 7.9e-13 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012160)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01040 AT4G01040
fragaria_vesca FvH4_7g16200
malus_domestica MD01G1078600.v1.1 MD07G1147600.v1.1
prunus_persica Prupe.2G185600_v2.0.a1 Prupe.2G185600_v2.0.a1
pyrus_communis pycom01g10710
rosa_chinensis RchiOBHm_Chr1g0359211
rosa_laevigata RLG00000027893
rosa_multiflora Rmu_co8274979.1_g000001 Rmu_ssc0000135.1_g000019
rosa_roxburghii Rroxscaffold_4G00297250
rosa_rugosa Rorug01G0268400
rosa_samantha Rh1AG282300 Rh1BG248700 Rh1CG266500 Rh1DG277700
rosa_wichuraiana Rw1G025070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 623
Acc65I GGTACC 1 cut(s) 878
AccB1I GGYRCC 2 cut(s) 23, 878
AciI CCGC 5 cut(s) 47, 64, 190, 679, 1126
AclWI GGATC 3 cut(s) 271, 500, 857
AcoI YGGCCR 2 cut(s) 12, 900
AcsI RAATTY 3 cut(s) 7, 361, 401
AcuI CTGAAG 4 cut(s) 150, 455, 642, 863
AcyI GRCGYC 1 cut(s) 24
AfaI GTAC 3 cut(s) 180, 188, 880
AfiI CCNNNNNNNGG 2 cut(s) 123, 968
AflIII ACRYGT 1 cut(s) 231
AjiI CACGTC 1 cut(s) 262
AjnI CCWGG 2 cut(s) 823, 961
AluBI AGCT 8 cut(s) 130, 344, 453, 551, 565, 724, 868, 1139
AluI AGCT 8 cut(s) 130, 344, 453, 551, 565, 724, 868, 1139
Alw21I GWGCWC 1 cut(s) 898
AlwI GGATC 3 cut(s) 271, 500, 857
AoxI GGCC 5 cut(s) 12, 244, 703, 812, 900
ApeKI GCWGC 2 cut(s) 565, 868
ApoI RAATTY 3 cut(s) 7, 361, 401
ArsI GACNNNNNNTTYG 2 cut(s) 60, 92
Asp700I GAANNNNTTC 1 cut(s) 552
Asp718I GGTACC 1 cut(s) 878
AspLEI GCGC 2 cut(s) 26, 1046
AspS9I GGNCC 6 cut(s) 447, 704, 743, 812, 827, 959
AsuHPI GGTGA 2 cut(s) 130, 970
AvaII GGWCC 4 cut(s) 447, 743, 827, 959
BaeI ACNNNNGTAYC 2 cut(s) 170, 203
BalI TGGCCA 1 cut(s) 902
BanI GGYRCC 2 cut(s) 23, 878
BauI CACGAG 1 cut(s) 182
Bbv12I GWGCWC 1 cut(s) 898
BbvI GCAGC 2 cut(s) 552, 855
BccI CCATC 3 cut(s) 773, 1023, 1111
BceAI ACGGC 1 cut(s) 37
BciT130I CCWGG 2 cut(s) 825, 963
BclI TGATCA 1 cut(s) 1075
BfaI CTAG 2 cut(s) 534, 1130
BfmI CTRYAG 1 cut(s) 614
BfoI RGCGCY 1 cut(s) 27
BglII AGATCT 2 cut(s) 628, 751
BisI GCNGC 4 cut(s) 64, 566, 869, 1127
BlsI GCNGC 4 cut(s) 65, 567, 870, 1128
BmcAI AGTACT 1 cut(s) 180
Bme1390I CCNGG 2 cut(s) 825, 963
Bme18I GGWCC 4 cut(s) 447, 743, 827, 959
BmgBI CACGTC 1 cut(s) 262
BmgT120I GGNCC 6 cut(s) 447, 704, 743, 812, 827, 959
BmiI GGNNCC 4 cut(s) 25, 448, 829, 880
BmrFI CCNGG 2 cut(s) 825, 963
BmsI GCATC 4 cut(s) 508, 628, 760, 930
BpmI CTGGAG 2 cut(s) 402, 848
Bsa29I ATCGAT 1 cut(s) 852
BsaAI YACGTR 1 cut(s) 232
BsaBI GATNNNNATC 1 cut(s) 1074
BsaHI GRCGYC 1 cut(s) 24
BsaJI CCNNGG 1 cut(s) 356
BsaWI WCCGGW 2 cut(s) 216, 980
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 968
Bse1I ACTGG 3 cut(s) 15, 319, 419
Bse8I GATNNNNATC 1 cut(s) 1074
BseBI CCWGG 2 cut(s) 825, 963
BseCI ATCGAT 1 cut(s) 852
BseDI CCNNGG 1 cut(s) 356
BseGI GGATG 3 cut(s) 110, 784, 945
BseJI GATNNNNATC 1 cut(s) 1074
BseLI CCNNNNNNNGG 2 cut(s) 123, 968
BseMII CTCAG 5 cut(s) 117, 189, 510, 822, 966
BseNI ACTGG 3 cut(s) 15, 319, 419
BseRI GAGGAG 1 cut(s) 106
BseXI GCAGC 2 cut(s) 552, 855
Bsh1285I CGRYCG 1 cut(s) 61
BshFI GGCC 5 cut(s) 14, 246, 705, 814, 902
BshNI GGYRCC 2 cut(s) 23, 878
BshVI ATCGAT 1 cut(s) 852
BsiEI CGRYCG 1 cut(s) 61
BsiHKAI GWGCWC 1 cut(s) 898
BsiSI CCGG 3 cut(s) 217, 238, 981
BslFI GGGAC 2 cut(s) 460, 813
BslI CCNNNNNNNGG 2 cut(s) 123, 968
BsmFI GGGAC 2 cut(s) 460, 813
BsnI GGCC 5 cut(s) 14, 246, 705, 814, 902
Bsp1286I GDGCHC 1 cut(s) 898
Bsp19I CCATGG 1 cut(s) 356
BspACI CCGC 5 cut(s) 47, 64, 190, 679, 1126
BspANI GGCC 5 cut(s) 14, 246, 705, 814, 902
BspCNI CTCAG 5 cut(s) 116, 188, 509, 821, 965
BspDI ATCGAT 1 cut(s) 852
BspLI GGNNCC 4 cut(s) 25, 448, 829, 880
BspPI GGATC 3 cut(s) 271, 500, 857
BspT107I GGYRCC 2 cut(s) 23, 878
BsrI ACTGG 3 cut(s) 15, 319, 419
BssECI CCNNGG 1 cut(s) 356
BssNI GRCGYC 1 cut(s) 24
BssSI CACGAG 1 cut(s) 182
BssT1I CCWWGG 1 cut(s) 356
Bst2BI CACGAG 1 cut(s) 182
Bst2UI CCWGG 2 cut(s) 825, 963
Bst4CI ACNGT 4 cut(s) 84, 618, 893, 1113
Bst6I CTCTTC 3 cut(s) 155, 431, 979
BstACI GRCGYC 1 cut(s) 24
BstAPI GCANNNNNTGC 3 cut(s) 518, 647, 682
BstBAI YACGTR 1 cut(s) 232
BstC8I GCNNGC 5 cut(s) 320, 648, 683, 904, 1020
BstDEI CTNAG 7 cut(s) 103, 175, 366, 496, 503, 808, 952
BstDSI CCRYGG 1 cut(s) 356
BstF5I GGATG 3 cut(s) 110, 784, 945
BstH2I RGCGCY 1 cut(s) 27
BstHHI GCGC 2 cut(s) 26, 1046
BstMCI CGRYCG 1 cut(s) 61
BstMWI GCNNNNNNNGC 7 cut(s) 30, 104, 518, 557, 647, 682, 730
BstNI CCWGG 2 cut(s) 825, 963
BstNSI RCATGY 2 cut(s) 650, 1162
BstSCI CCNGG 2 cut(s) 823, 961
BstSFI CTRYAG 1 cut(s) 614
BstV1I GCAGC 2 cut(s) 552, 855
BstX2I RGATCY 4 cut(s) 276, 492, 628, 751
BstYI RGATCY 4 cut(s) 276, 492, 628, 751
Bsu15I ATCGAT 1 cut(s) 852
BsuRI GGCC 5 cut(s) 14, 246, 705, 814, 902
BsuTUI ATCGAT 1 cut(s) 852
BtgI CCRYGG 1 cut(s) 356
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 3 cut(s) 110, 784, 945
BtsI GCAGTG 1 cut(s) 641
BtsIMutI CAGTG 5 cut(s) 22, 282, 641, 898, 1065
Cac8I GCNNGC 5 cut(s) 320, 648, 683, 904, 1020
CfoI GCGC 2 cut(s) 26, 1046
Cfr13I GGNCC 6 cut(s) 447, 704, 743, 812, 827, 959
ClaI ATCGAT 1 cut(s) 852
Csp6I GTAC 3 cut(s) 179, 187, 879
CviAII CATG 6 cut(s) 357, 647, 734, 1088, 1123, 1159
CviQI GTAC 3 cut(s) 179, 187, 879
DdeI CTNAG 7 cut(s) 103, 175, 366, 496, 503, 808, 952
DinI GGCGCC 1 cut(s) 25
EaeI YGGCCR 2 cut(s) 12, 900
Eam1104I CTCTTC 3 cut(s) 155, 431, 979
EarI CTCTTC 3 cut(s) 155, 431, 979
Eco130I CCWWGG 1 cut(s) 356
Eco147I AGGCCT 1 cut(s) 246
Eco47I GGWCC 4 cut(s) 447, 743, 827, 959
Eco57I CTGAAG 4 cut(s) 150, 455, 642, 863
EcoO109I RGGNCCY 3 cut(s) 812, 827, 959
EcoRI GAATTC 1 cut(s) 361
EcoRII CCWGG 2 cut(s) 823, 961
EcoT14I CCWWGG 1 cut(s) 356
EgeI GGCGCC 1 cut(s) 25
EheI GGCGCC 1 cut(s) 25
ErhI CCWWGG 1 cut(s) 356
FaeI CATG 6 cut(s) 360, 650, 737, 1091, 1126, 1162
FalI AAGNNNNNCTT 2 cut(s) 665, 697
FaqI GGGAC 2 cut(s) 460, 813
FatI CATG 6 cut(s) 356, 646, 733, 1087, 1122, 1158
FbaI TGATCA 1 cut(s) 1075
Fnu4HI GCNGC 4 cut(s) 64, 566, 869, 1127
FokI GGATG 3 cut(s) 97, 791, 932
Fsp4HI GCNGC 4 cut(s) 64, 566, 869, 1127
FspBI CTAG 2 cut(s) 534, 1130
GlaI GCGC 2 cut(s) 25, 1045
GluI GCNGC 4 cut(s) 64, 566, 869, 1127
GsuI CTGGAG 2 cut(s) 402, 848
HaeII RGCGCY 1 cut(s) 27
HaeIII GGCC 5 cut(s) 14, 246, 705, 814, 902
HapII CCGG 3 cut(s) 217, 238, 981
HhaI GCGC 2 cut(s) 26, 1046
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 6 cut(s) 360, 650, 737, 1091, 1126, 1162
Hin6I GCGC 2 cut(s) 24, 1044
HinP1I GCGC 2 cut(s) 24, 1044
HindIII AAGCTT 1 cut(s) 549
HinfI GANTC 5 cut(s) 75, 224, 439, 783, 854
HpaII CCGG 3 cut(s) 217, 238, 981
HphI GGTGA 2 cut(s) 130, 970
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 9 cut(s) 58, 126, 207, 304, 435, 499, 661, 718, 843
Hpy188III TCNNGA 4 cut(s) 461, 545, 954, 1187
Hpy8I GTNNAC 1 cut(s) 187
Hpy99I CGWCG 3 cut(s) 88, 161, 263
HpyAV CCTTC 2 cut(s) 151, 458
HpyCH4III ACNGT 4 cut(s) 84, 618, 893, 1113
HpyCH4IV ACGT 3 cut(s) 231, 261, 797
HpyCH4V TGCA 7 cut(s) 641, 646, 685, 733, 836, 1006, 1022
HpyF10VI GCNNNNNNNGC 7 cut(s) 30, 104, 518, 557, 647, 682, 730
HpyF3I CTNAG 7 cut(s) 103, 175, 366, 496, 503, 808, 952
HpySE526I ACGT 3 cut(s) 231, 261, 797
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 6 cut(s) 360, 650, 737, 1091, 1126, 1162
HspAI GCGC 2 cut(s) 24, 1044
KasI GGCGCC 1 cut(s) 23
KpnI GGTACC 1 cut(s) 882
Ksp22I TGATCA 1 cut(s) 1075
LmnI GCTCC 2 cut(s) 208, 729
Lsp1109I GCAGC 2 cut(s) 552, 855
LweI GCATC 4 cut(s) 508, 628, 760, 930
MaeI CTAG 2 cut(s) 534, 1130
MaeII ACGT 3 cut(s) 231, 261, 797
MaeIII GTNAC 4 cut(s) 17, 262, 652, 1107
MboII GAAGA 9 cut(s) 47, 142, 145, 156, 448, 746, 996, 1051, 1196
MflI RGATCY 4 cut(s) 276, 492, 628, 751
MhlI GDGCHC 1 cut(s) 898
MlsI TGGCCA 1 cut(s) 902
MluCI AATT 9 cut(s) 7, 196, 361, 401, 456, 537, 925, 1116, 1173
MluNI TGGCCA 1 cut(s) 902
Mly113I GGCGCC 1 cut(s) 24
MlyI GAGTC 3 cut(s) 84, 233, 448
Mox20I TGGCCA 1 cut(s) 902
MroXI GAANNNNTTC 1 cut(s) 552
MscI TGGCCA 1 cut(s) 902
MseI TTAA 3 cut(s) 252, 393, 455
MslI CAYNNNNRTG 1 cut(s) 1027
Msp20I TGGCCA 1 cut(s) 902
MspA1I CMGCKG 1 cut(s) 868
MspI CCGG 3 cut(s) 217, 238, 981
MspR9I CCNGG 2 cut(s) 825, 963
MvaI CCWGG 2 cut(s) 825, 963
MwoI GCNNNNNNNGC 7 cut(s) 30, 104, 518, 557, 647, 682, 730
NarI GGCGCC 1 cut(s) 24
NcoI CCATGG 1 cut(s) 356
NlaIII CATG 6 cut(s) 360, 650, 737, 1091, 1126, 1162
NlaIV GGNNCC 4 cut(s) 25, 448, 829, 880
NmeAIII GCCGAG 2 cut(s) 75, 897
NmuCI GTSAC 3 cut(s) 17, 262, 652
NspI RCATGY 2 cut(s) 650, 1162
PaeI GCATGC 1 cut(s) 650
PceI AGGCCT 1 cut(s) 246
PcsI WCGNNNNNNNCGW 2 cut(s) 255, 308
PdmI GAANNNNTTC 1 cut(s) 552
PfeI GAWTC 2 cut(s) 783, 854
PflFI GACNNNGTC 2 cut(s) 74, 223
PkrI GCNGC 4 cut(s) 65, 567, 870, 1128
Ple19I CGATCG 1 cut(s) 61
PleI GAGTC 3 cut(s) 83, 232, 447
PluTI GGCGCC 1 cut(s) 27
PpsI GAGTC 3 cut(s) 83, 232, 447
Ppu21I YACGTR 1 cut(s) 232
PpuMI RGGWCCY 2 cut(s) 827, 959
PsiI TTATAA 1 cut(s) 623
Psp5II RGGWCCY 2 cut(s) 827, 959
Psp6I CCWGG 2 cut(s) 823, 961
PspGI CCWGG 2 cut(s) 823, 961
PspN4I GGNNCC 4 cut(s) 25, 448, 829, 880
PspPI GGNCC 6 cut(s) 447, 704, 743, 812, 827, 959
PspPPI RGGWCCY 2 cut(s) 827, 959
PsuI RGATCY 4 cut(s) 276, 492, 628, 751
PsyI GACNNNGTC 2 cut(s) 74, 223
PvuI CGATCG 1 cut(s) 61
PvuII CAGCTG 1 cut(s) 868
RsaI GTAC 3 cut(s) 180, 188, 880
RsaNI GTAC 3 cut(s) 179, 187, 879
RseI CAYNNNNRTG 1 cut(s) 1027
SaqAI TTAA 3 cut(s) 252, 393, 455
SatI GCNGC 4 cut(s) 64, 566, 869, 1127
Sau96I GGNCC 6 cut(s) 447, 704, 743, 812, 827, 959
ScaI AGTACT 1 cut(s) 180
SchI GAGTC 3 cut(s) 84, 233, 448
ScrFI CCNGG 2 cut(s) 825, 963
SduI GDGCHC 1 cut(s) 898
SfaNI GCATC 4 cut(s) 508, 628, 760, 930
SfcI CTRYAG 1 cut(s) 614
SfoI GGCGCC 1 cut(s) 25
SinI GGWCC 4 cut(s) 447, 743, 827, 959
SmiMI CAYNNNNRTG 1 cut(s) 1027
SphI GCATGC 1 cut(s) 650
Sse9I AATT 9 cut(s) 7, 196, 361, 401, 456, 537, 925, 1116, 1173
SseBI AGGCCT 1 cut(s) 246
SsiI CCGC 5 cut(s) 47, 64, 190, 679, 1126
SspDI GGCGCC 1 cut(s) 23
SspMI CTAG 2 cut(s) 534, 1130
StuI AGGCCT 1 cut(s) 246
StyD4I CCNGG 2 cut(s) 823, 961
StyI CCWWGG 1 cut(s) 356
TaaI ACNGT 4 cut(s) 84, 618, 893, 1113
TaiI ACGT 3 cut(s) 234, 264, 800
TaqI TCGA 4 cut(s) 78, 852, 1027, 1163
TaqII GACCGA 1 cut(s) 87
TasI AATT 9 cut(s) 7, 196, 361, 401, 456, 537, 925, 1116, 1173
TatI WGTACW 1 cut(s) 178
TauI GCSGC 2 cut(s) 66, 1129
TfiI GAWTC 2 cut(s) 783, 854
Tru1I TTAA 3 cut(s) 252, 393, 455
Tru9I TTAA 3 cut(s) 252, 393, 455
TscAI CASTG 5 cut(s) 22, 289, 648, 898, 1072
TseFI GTSAC 3 cut(s) 17, 262, 652
TseI GCWGC 2 cut(s) 565, 868
Tsp45I GTSAC 3 cut(s) 17, 262, 652
TspDTI ATGAA 2 cut(s) 17, 746
TspGWI ACGGA 1 cut(s) 236
TspRI CASTG 5 cut(s) 22, 289, 648, 898, 1072
Tth111I GACNNNGTC 2 cut(s) 74, 223
VpaK11BI GGWCC 4 cut(s) 447, 743, 827, 959
XapI RAATTY 3 cut(s) 7, 361, 401
XceI RCATGY 2 cut(s) 650, 1162
XcmI CCANNNNNNNNNTGG 2 cut(s) 457, 872
XmnI GAANNNNTTC 1 cut(s) 552
XspI CTAG 2 cut(s) 534, 1130
ZrmI AGTACT 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.