Rroxscaffold_4G00297250

Chitinase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0012160
Physical Location & Seq
Forward (+)
17179941 .. 17182677
2737 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00297250.1

Sequence Viewer

Length: 1119 bp
ATGACCAGTCAAGGGACCAGCTTAATTTTGGAAGGCAGACATAATGCTGATATTGGGTGGATCTCAGACCTTAGAATAGCAGGAGACGCTTGGGTATTGCCTAGAATTGTTCTGGAAGCTTTTCCAGCAGAGCTGCTTACAAAGAAAAAGCAGAGGAATAAGGCTATTGATCTTATAGTAAGCGAGTGCAAGGAAATGGGCTATGATGGAGTTGTGCTGGAATCTTGGTCAAGGTGGGCAGCTTACCGTGTTTTGCATGATCCAAAGATGCGGAATTTGGCATTACAGTTTATAAAAGATCTTGGAGATGCACTGCATGCTGTGACTTCAGAGAGGAACAATAAGCACCGCTTGCAGTTGGTGTATGTTATAGGCCCACCACATTCAGAGAAGCTCCAAGTGCATGATTTTGGACCTAAAGATCTTCATAGCTTGAGTGATGCTGTGGATGGATTCTCACTTATGACGTATGACTTCTCAGGCCCTCATAATCCAGGTCCCAATGCACCTTTGAAGTGGATCGATTCCACCCTCCAGCTGCTTCTTGGTACCAATAAAAACAGTGCTCTAGCCAGCAAGATACTTCTCGGCATCAATTTCTATGGAAATGATTACATCCTCTCAGGAGGTCCTGGTGGTGGTGCTATCACCGGAAGAGATTACATCTCCTTGTTGGAGAAGCACAAGCCTGCATTTCGATGGGAAAAGAACAGCGCAGAACATTTGTTCTTCTACACTGATAATGATCATAACAATCATGCTGTGTTCTACCCATCGCTACTGTCAATTTCCATGCGGCTAGAGGAAGCTCGTAAATGGGGTTGTGGCATCTCGATATGGGAAATTGGGCAAGCATGTACTGGAGACTTTCACTTCTGCGGACTTCTTTTCTCAACCACACAATGGACGAAGCAAAGTCTTGTGCTTTCAATCAACAACATAATTTATCAACAAACCTCTCAATCCTCAACACTACGTGTCATGCACCGAATGGCCAAGTTTCTCACCTTGCATGACACATCAGCACCACCCTCCCACCCAAAGACCAAAAAGACACCAGCCAAGTTGCACTCACTGAACAAGCTAAGGCTTGTAGAGCCTTGTATCATCTTAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

372

Amino Acids

41.76

Weight (kDa)

8.66

Isoelectric Point (pI)

44.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 45 - 284 1.4e-17 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012160)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01040 AT4G01040
fragaria_vesca FvH4_7g16200
malus_domestica MD01G1078600.v1.1 MD07G1147600.v1.1
prunus_persica Prupe.2G185600_v2.0.a1 Prupe.2G185600_v2.0.a1
pyrus_communis pycom01g10710
rosa_chinensis RchiOBHm_Chr1g0359211
rosa_laevigata RLG00000027893
rosa_multiflora Rmu_co8274979.1_g000001 Rmu_ssc0000135.1_g000019
rosa_roxburghii Rroxscaffold_4G00297250
rosa_rugosa Rorug01G0268400
rosa_samantha Rh1AG282300 Rh1BG248700 Rh1CG266500 Rh1DG277700
rosa_wichuraiana Rw1G025070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 293
Acc65I GGTACC 1 cut(s) 548
AccB1I GGYRCC 1 cut(s) 548
AccB7I CCANNNNNTGG 1 cut(s) 903
AciI CCGC 4 cut(s) 271, 349, 796, 879
AclWI GGATC 3 cut(s) 68, 254, 527
AcoI YGGCCR 1 cut(s) 993
AcsI RAATTY 1 cut(s) 274
AcuI CTGAAG 1 cut(s) 312
AdeI CACNNNGTG 1 cut(s) 977
AfaI GTAC 2 cut(s) 550, 859
AfiI CCNNNNNNNGG 3 cut(s) 12, 638, 903
AflIII ACRYGT 1 cut(s) 976
AgsI TTSAA 2 cut(s) 514, 930
AjnI CCWGG 2 cut(s) 493, 631
AluBI AGCT 9 cut(s) 21, 119, 133, 242, 394, 432, 538, 809, 1084
AluI AGCT 9 cut(s) 21, 119, 133, 242, 394, 432, 538, 809, 1084
Alw21I GWGCWC 1 cut(s) 568
Alw26I GTCTC 2 cut(s) 78, 858
AlwI GGATC 3 cut(s) 68, 254, 527
AoxI GGCC 3 cut(s) 373, 481, 993
ApeKI GCWGC 3 cut(s) 133, 239, 538
ApoI RAATTY 1 cut(s) 274
Asp700I GAANNNNTTC 1 cut(s) 120
Asp718I GGTACC 1 cut(s) 548
AspLEI GCGC 1 cut(s) 716
AspS9I GGNCC 6 cut(s) 15, 374, 413, 482, 497, 629
AsuHPI GGTGA 2 cut(s) 640, 997
AvaII GGWCC 4 cut(s) 15, 413, 497, 629
BalI TGGCCA 1 cut(s) 995
BanI GGYRCC 1 cut(s) 548
Bbv12I GWGCWC 1 cut(s) 568
BbvI GCAGC 3 cut(s) 120, 251, 525
BccI CCATC 4 cut(s) 200, 443, 693, 781
BciT130I CCWGG 2 cut(s) 495, 633
BclI TGATCA 1 cut(s) 745
BcoDI GTCTC 2 cut(s) 78, 858
BfaI CTAG 3 cut(s) 102, 569, 800
BglII AGATCT 2 cut(s) 298, 421
BisI GCNGC 4 cut(s) 134, 240, 539, 797
BlsI GCNGC 4 cut(s) 135, 241, 540, 798
Bme1390I CCNGG 2 cut(s) 495, 633
Bme18I GGWCC 4 cut(s) 15, 413, 497, 629
BmgT120I GGNCC 6 cut(s) 15, 374, 413, 482, 497, 629
BmiI GGNNCC 3 cut(s) 16, 499, 550
BmrFI CCNGG 2 cut(s) 495, 633
BmsI GCATC 5 cut(s) 258, 298, 430, 600, 837
BpmI CTGGAG 2 cut(s) 518, 882
Bpu10I CCTNAGC 1 cut(s) 1085
BpuEI CTTGAG 1 cut(s) 454
Bsa29I ATCGAT 1 cut(s) 522
BsaAI YACGTR 1 cut(s) 977
BsaBI GATNNNNATC 1 cut(s) 744
BsaWI WCCGGW 1 cut(s) 650
Bsc4I CCNNNNNNNGG 3 cut(s) 12, 638, 903
Bse1I ACTGG 2 cut(s) 6, 865
Bse8I GATNNNNATC 1 cut(s) 744
BseBI CCWGG 2 cut(s) 495, 633
BseCI ATCGAT 1 cut(s) 522
BseGI GGATG 2 cut(s) 454, 615
BseJI GATNNNNATC 1 cut(s) 744
BseLI CCNNNNNNNGG 3 cut(s) 12, 638, 903
BseMII CTCAG 3 cut(s) 78, 492, 636
BseNI ACTGG 2 cut(s) 6, 865
BseXI GCAGC 3 cut(s) 120, 251, 525
BshFI GGCC 3 cut(s) 375, 483, 995
BshNI GGYRCC 1 cut(s) 548
BshVI ATCGAT 1 cut(s) 522
BsiHKAI GWGCWC 1 cut(s) 568
BsiSI CCGG 1 cut(s) 651
BslFI GGGAC 2 cut(s) 28, 483
BslI CCNNNNNNNGG 3 cut(s) 12, 638, 903
BsmAI GTCTC 2 cut(s) 78, 858
BsmBI CGTCTC 1 cut(s) 78
BsmFI GGGAC 2 cut(s) 28, 483
BsnI GGCC 3 cut(s) 375, 483, 995
Bsp1286I GDGCHC 1 cut(s) 568
Bsp143I GATC 7 cut(s) 60, 169, 259, 298, 421, 519, 745
BspACI CCGC 4 cut(s) 271, 349, 796, 879
BspANI GGCC 3 cut(s) 375, 483, 995
BspCNI CTCAG 3 cut(s) 77, 491, 635
BspDI ATCGAT 1 cut(s) 522
BspLI GGNNCC 3 cut(s) 16, 499, 550
BspPI GGATC 3 cut(s) 68, 254, 527
BspT107I GGYRCC 1 cut(s) 548
BsrI ACTGG 2 cut(s) 6, 865
BssMI GATC 7 cut(s) 60, 169, 259, 298, 421, 519, 745
Bst2UI CCWGG 2 cut(s) 495, 633
Bst4CI ACNGT 4 cut(s) 248, 288, 563, 783
Bst6I CTCTTC 1 cut(s) 649
BstAPI GCANNNNNTGC 2 cut(s) 317, 352
BstBAI YACGTR 1 cut(s) 977
BstC8I GCNNGC 5 cut(s) 318, 353, 574, 690, 852
BstDEI CTNAG 6 cut(s) 64, 71, 478, 622, 1085, 1111
BstF5I GGATG 2 cut(s) 454, 615
BstHHI GCGC 1 cut(s) 716
BstKTI GATC 7 cut(s) 63, 172, 262, 301, 424, 522, 748
BstMAI GTCTC 2 cut(s) 78, 858
BstMBI GATC 7 cut(s) 60, 169, 259, 298, 421, 519, 745
BstMWI GCNNNNNNNGC 6 cut(s) 86, 125, 317, 352, 400, 1096
BstNI CCWGG 2 cut(s) 495, 633
BstNSI RCATGY 2 cut(s) 320, 858
BstSCI CCNGG 2 cut(s) 493, 631
BstV1I GCAGC 3 cut(s) 120, 251, 525
BstX2I RGATCY 3 cut(s) 60, 298, 421
BstYI RGATCY 3 cut(s) 60, 298, 421
Bsu15I ATCGAT 1 cut(s) 522
BsuRI GGCC 3 cut(s) 375, 483, 995
BsuTUI ATCGAT 1 cut(s) 522
BtgZI GCGATG 1 cut(s) 759
BtsCI GGATG 2 cut(s) 454, 615
BtsI GCAGTG 1 cut(s) 311
BtsIMutI CAGTG 4 cut(s) 311, 568, 735, 1073
Cac8I GCNNGC 5 cut(s) 318, 353, 574, 690, 852
CfoI GCGC 1 cut(s) 716
Cfr13I GGNCC 6 cut(s) 15, 374, 413, 482, 497, 629
ClaI ATCGAT 1 cut(s) 522
CseI GACGC 1 cut(s) 95
Csp6I GTAC 2 cut(s) 549, 858
CviAII CATG 8 cut(s) 257, 317, 404, 758, 793, 855, 982, 1013
CviQI GTAC 2 cut(s) 549, 858
DdeI CTNAG 6 cut(s) 64, 71, 478, 622, 1085, 1111
DpnI GATC 7 cut(s) 62, 171, 261, 300, 423, 521, 747
DpnII GATC 7 cut(s) 60, 169, 259, 298, 421, 519, 745
DraIII CACNNNGTG 1 cut(s) 977
EaeI YGGCCR 1 cut(s) 993
Eam1104I CTCTTC 1 cut(s) 649
EarI CTCTTC 1 cut(s) 649
Eco47I GGWCC 4 cut(s) 15, 413, 497, 629
Eco57I CTGAAG 1 cut(s) 312
EcoO109I RGGNCCY 3 cut(s) 482, 497, 629
EcoRII CCWGG 2 cut(s) 493, 631
Esp3I CGTCTC 1 cut(s) 78
FaeI CATG 8 cut(s) 260, 320, 407, 761, 796, 858, 985, 1016
FalI AAGNNNNNCTT 2 cut(s) 335, 367
FaqI GGGAC 2 cut(s) 28, 483
FatI CATG 8 cut(s) 256, 316, 403, 757, 792, 854, 981, 1012
FbaI TGATCA 1 cut(s) 745
Fnu4HI GCNGC 4 cut(s) 134, 240, 539, 797
FokI GGATG 2 cut(s) 461, 602
Fsp4HI GCNGC 4 cut(s) 134, 240, 539, 797
FspBI CTAG 3 cut(s) 102, 569, 800
GlaI GCGC 1 cut(s) 715
GluI GCNGC 4 cut(s) 134, 240, 539, 797
GsuI CTGGAG 2 cut(s) 518, 882
HaeIII GGCC 3 cut(s) 375, 483, 995
HapII CCGG 1 cut(s) 651
HgaI GACGC 1 cut(s) 95
HhaI GCGC 1 cut(s) 716
Hin1II CATG 8 cut(s) 260, 320, 407, 761, 796, 858, 985, 1016
Hin6I GCGC 1 cut(s) 714
HinP1I GCGC 1 cut(s) 714
HindIII AAGCTT 1 cut(s) 117
HinfI GANTC 3 cut(s) 221, 453, 524
HpaII CCGG 1 cut(s) 651
HphI GGTGA 2 cut(s) 640, 997
Hpy188I TCNGA 3 cut(s) 67, 331, 388
Hpy188III TCNNGA 3 cut(s) 113, 624, 832
HpyAV CCTTC 1 cut(s) 26
HpyCH4III ACNGT 4 cut(s) 248, 288, 563, 783
HpyCH4IV ACGT 2 cut(s) 467, 976
HpyF10VI GCNNNNNNNGC 6 cut(s) 86, 125, 317, 352, 400, 1096
HpyF3I CTNAG 6 cut(s) 64, 71, 478, 622, 1085, 1111
HpySE526I ACGT 2 cut(s) 467, 976
Hsp92II CATG 8 cut(s) 260, 320, 407, 761, 796, 858, 985, 1016
HspAI GCGC 1 cut(s) 714
KpnI GGTACC 1 cut(s) 552
Ksp22I TGATCA 1 cut(s) 745
Kzo9I GATC 7 cut(s) 60, 169, 259, 298, 421, 519, 745
LmnI GCTCC 1 cut(s) 399
Lsp1109I GCAGC 3 cut(s) 120, 251, 525
LweI GCATC 5 cut(s) 258, 298, 430, 600, 837
MaeI CTAG 3 cut(s) 102, 569, 800
MaeII ACGT 2 cut(s) 467, 976
MaeIII GTNAC 1 cut(s) 322
MalI GATC 7 cut(s) 62, 171, 261, 300, 423, 521, 747
MboI GATC 7 cut(s) 60, 169, 259, 298, 421, 519, 745
MboII GAAGA 3 cut(s) 416, 666, 721
MflI RGATCY 3 cut(s) 60, 298, 421
MhlI GDGCHC 1 cut(s) 568
MlsI TGGCCA 1 cut(s) 995
MluCI AATT 7 cut(s) 24, 105, 274, 595, 786, 843, 942
MluNI TGGCCA 1 cut(s) 995
MmeI TCCRAC 1 cut(s) 654
Mox20I TGGCCA 1 cut(s) 995
MroXI GAANNNNTTC 1 cut(s) 120
MscI TGGCCA 1 cut(s) 995
MseI TTAA 1 cut(s) 23
MslI CAYNNNNRTG 1 cut(s) 697
Msp20I TGGCCA 1 cut(s) 995
MspA1I CMGCKG 1 cut(s) 538
MspI CCGG 1 cut(s) 651
MspR9I CCNGG 2 cut(s) 495, 633
MvaI CCWGG 2 cut(s) 495, 633
MwoI GCNNNNNNNGC 6 cut(s) 86, 125, 317, 352, 400, 1096
NdeII GATC 7 cut(s) 60, 169, 259, 298, 421, 519, 745
NlaIII CATG 8 cut(s) 260, 320, 407, 761, 796, 858, 985, 1016
NlaIV GGNNCC 3 cut(s) 16, 499, 550
NmeAIII GCCGAG 1 cut(s) 567
NmuCI GTSAC 1 cut(s) 322
NspI RCATGY 2 cut(s) 320, 858
PaeI GCATGC 1 cut(s) 320
PdmI GAANNNNTTC 1 cut(s) 120
PfeI GAWTC 3 cut(s) 221, 453, 524
PflMI CCANNNNNTGG 1 cut(s) 903
PkrI GCNGC 4 cut(s) 135, 241, 540, 798
Ppu21I YACGTR 1 cut(s) 977
PpuMI RGGWCCY 2 cut(s) 497, 629
PsiI TTATAA 1 cut(s) 293
Psp5II RGGWCCY 2 cut(s) 497, 629
Psp6I CCWGG 2 cut(s) 493, 631
PspGI CCWGG 2 cut(s) 493, 631
PspN4I GGNNCC 3 cut(s) 16, 499, 550
PspPI GGNCC 6 cut(s) 15, 374, 413, 482, 497, 629
PspPPI RGGWCCY 2 cut(s) 497, 629
PsuI RGATCY 3 cut(s) 60, 298, 421
PvuII CAGCTG 1 cut(s) 538
RsaI GTAC 2 cut(s) 550, 859
RsaNI GTAC 2 cut(s) 549, 858
RseI CAYNNNNRTG 1 cut(s) 697
SaqAI TTAA 1 cut(s) 23
SatI GCNGC 4 cut(s) 134, 240, 539, 797
Sau3AI GATC 7 cut(s) 60, 169, 259, 298, 421, 519, 745
Sau96I GGNCC 6 cut(s) 15, 374, 413, 482, 497, 629
ScrFI CCNGG 2 cut(s) 495, 633
SduI GDGCHC 1 cut(s) 568
SfaNI GCATC 5 cut(s) 258, 298, 430, 600, 837
SinI GGWCC 4 cut(s) 15, 413, 497, 629
SmiMI CAYNNNNRTG 1 cut(s) 697
SmlI CTYRAG 1 cut(s) 433
SmoI CTYRAG 1 cut(s) 433
SphI GCATGC 1 cut(s) 320
Sse9I AATT 7 cut(s) 24, 105, 274, 595, 786, 843, 942
SsiI CCGC 4 cut(s) 271, 349, 796, 879
SspMI CTAG 3 cut(s) 102, 569, 800
StyD4I CCNGG 2 cut(s) 493, 631
TaaI ACNGT 4 cut(s) 248, 288, 563, 783
TaiI ACGT 2 cut(s) 470, 979
TaqI TCGA 3 cut(s) 522, 697, 833
TasI AATT 7 cut(s) 24, 105, 274, 595, 786, 843, 942
TatI WGTACW 1 cut(s) 857
TauI GCSGC 1 cut(s) 799
TfiI GAWTC 3 cut(s) 221, 453, 524
Tru1I TTAA 1 cut(s) 23
Tru9I TTAA 1 cut(s) 23
TscAI CASTG 4 cut(s) 318, 568, 742, 1080
TseFI GTSAC 1 cut(s) 322
TseI GCWGC 3 cut(s) 133, 239, 538
Tsp45I GTSAC 1 cut(s) 322
TspDTI ATGAA 1 cut(s) 416
TspRI CASTG 4 cut(s) 318, 568, 742, 1080
Van91I CCANNNNNTGG 1 cut(s) 903
VpaK11BI GGWCC 4 cut(s) 15, 413, 497, 629
XapI RAATTY 1 cut(s) 274
XceI RCATGY 2 cut(s) 320, 858
XcmI CCANNNNNNNNNTGG 2 cut(s) 25, 542
XmnI GAANNNNTTC 1 cut(s) 120
XspI CTAG 3 cut(s) 102, 569, 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.