MD07G1147600.v1.1

Chitinase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0012160
Physical Location & Seq
Reverse (-)
21537226 .. 21541249
4024 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1147600.v1.1.491

Sequence Viewer

Length: 1281 bp
ATGGCCAAGAAAAGAGAGCGCCGAGTCGCACCGAGTTCGAACCGCGGCGAAGACCGAGTAGAACCTTCGGGACAACTCCGCGACGCAAACGGGCAGCACATAGTCACCTTCGTCCTCTTCTTCTGCGTAGCTGTAGCAACCGCCGTGCTCGCCTACCGCATCAAATATGCTCCGACGGCGAACCCGACGAATTACGCTGTCTACGAGCGAGGAATCGTGAAACCTGATGTGAATTACCAGGAGATCCTCACTGAAAATGGAAAGGTTTCCGAAAATACGAGTGGCAGGCACTTTGTATATCCTGTATTGGCCTACATTACTCCATGGAATTCTAAGGGCTATGATATGGCCAAATGGTTCAACTCCAAGTTTACTCATTTATCTCCAATTTGGTATGACCTAAAGAGCCAAGGCAACAGCTTAATTCTGGAAGGTAGGCATAATGCTGATATGGGATGGATCTCAGATCTAAGGATGGCAGGAGATGCTTGGGTATTGCCTAGAGTTGTTCTGGAAGCATTTCCAGCAGAGCTGCTTACAAAGAAGAAGCAGAGGAGTAAAGCTATTAATCTTATAGTAAACGAGTGCAAGGAGATGAGATATGACGGTATTGTACTGGAATCTTGGTCAAGGTGGGCAGCTTACAGAATTTTGCATGATCCAAGCTTGCGGAATTTGGCATTGCAGTTTATAAAAGAGCTTGGTGATGCATTACATGGTGTGAGCTTAGAGAGGAATGATAAGCAACGGTTGCAATTAGTCTATGTTATTGGTCCGCCACATTCAGAGAAGCTCCAACCGCATGATTTTGGACCTAAAGATCTTCAGAGCTTGGATGATGCTGTGGATGGATTCTCGCTTATGACGTATGATTTCTCGGGACCCAATAATCCTGGTCCCAATGCACCTCTGAAGTGGATTCATTCTACCATGCAGCTGCTCCTTGGTACCAATAAAAACAGTGCTCTAGCCTGCAAGATATTTCTTGGCATCAATTTCTACGGCAATGATTTCGTCCTCACAGGAGGTCCGGGTGGTGGTGCTATCACTGGAAGGGATTACCTGTCCTTGTTGGAGAAGCACAAGCCCGAGTTGGAATGGGAAAAGAACAGCGCAGAACATTTGTTCTTTTACTCCGATGATGAAAGTAATTACCACGCTGTGTTCTATCCATCACCAATGTCAATTTCTGTGCGGCTAGAGGAAGCTCGCAAATGGGGATGCGGCATCTCAATTTGGGAAATTGGGCAAGGTTTGGATTACTTTTTCGATCTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

427

Amino Acids

48.26

Weight (kDa)

6.65

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 178 - 417 3.9e-17 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012160)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01040 AT4G01040
fragaria_vesca FvH4_7g16200
malus_domestica MD01G1078600.v1.1 MD07G1147600.v1.1
prunus_persica Prupe.2G185600_v2.0.a1 Prupe.2G185600_v2.0.a1
pyrus_communis pycom01g10710
rosa_chinensis RchiOBHm_Chr1g0359211
rosa_laevigata RLG00000027893
rosa_multiflora Rmu_co8274979.1_g000001 Rmu_ssc0000135.1_g000019
rosa_roxburghii Rroxscaffold_4G00297250
rosa_rugosa Rorug01G0268400
rosa_samantha Rh1AG282300 Rh1BG248700 Rh1CG266500 Rh1DG277700
rosa_wichuraiana Rw1G025070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 692
Acc65I GGTACC 1 cut(s) 947
AccB1I GGYRCC 1 cut(s) 947
AccI GTMKAC 1 cut(s) 201
AccII CGCG 2 cut(s) 45, 81
AclWI GGATC 3 cut(s) 238, 467, 653
AcoI YGGCCR 2 cut(s) 3, 348
AcsI RAATTY 3 cut(s) 328, 648, 673
AcuI CTGAAG 2 cut(s) 809, 932
AdeI CACNNNGTG 1 cut(s) 1162
AfaI GTAC 2 cut(s) 615, 949
AfiI CCNNNNNNNGG 1 cut(s) 1037
AgsI TTSAA 1 cut(s) 361
AjnI CCWGG 2 cut(s) 237, 892
AjuI GAANNNNNNNTTGG 2 cut(s) 1171, 1203
Alw21I GWGCWC 2 cut(s) 150, 967
AlwI GGATC 3 cut(s) 238, 467, 653
Ama87I CYCGRG 2 cut(s) 877, 1088
AoxI GGCC 3 cut(s) 3, 309, 348
ApeKI GCWGC 5 cut(s) 94, 532, 638, 934, 937
ApoI RAATTY 3 cut(s) 328, 648, 673
AseI ATTAAT 1 cut(s) 567
Asp700I GAANNNNTTC 2 cut(s) 265, 519
Asp718I GGTACC 1 cut(s) 947
AspLEI GCGC 2 cut(s) 21, 1115
AspS9I GGNCC 5 cut(s) 773, 812, 881, 896, 1028
AsuC2I CCSGG 1 cut(s) 1032
AsuHPI GGTGA 3 cut(s) 97, 716, 1167
AsuII TTCGAA 1 cut(s) 38
AvaI CYCGRG 2 cut(s) 877, 1088
AvaII GGWCC 5 cut(s) 773, 812, 881, 896, 1028
BalI TGGCCA 2 cut(s) 5, 350
BanI GGYRCC 1 cut(s) 947
BbsI GAAGAC 1 cut(s) 57
Bbv12I GWGCWC 2 cut(s) 150, 967
BbvI GCAGC 5 cut(s) 106, 519, 650, 924, 946
BccI CCATC 4 cut(s) 450, 469, 842, 1180
BceAI ACGGC 3 cut(s) 128, 192, 1018
BcgI CGANNNNNNTGC 4 cut(s) 18, 52, 994, 1028
BciT130I CCWGG 2 cut(s) 239, 894
BcnI CCSGG 1 cut(s) 1032
BfaI CTAG 3 cut(s) 501, 968, 1199
BfmI CTRYAG 1 cut(s) 132
BfoI RGCGCY 1 cut(s) 22
BglII AGATCT 2 cut(s) 466, 820
BisI GCNGC 8 cut(s) 46, 95, 533, 639, 935, 938, 1196, 1225
BlsI GCNGC 8 cut(s) 47, 96, 534, 640, 936, 939, 1197, 1226
Bme1390I CCNGG 3 cut(s) 239, 894, 1032
Bme18I GGWCC 5 cut(s) 773, 812, 881, 896, 1028
BmeT110I CYCGRG 2 cut(s) 877, 1088
BmgT120I GGNCC 5 cut(s) 773, 812, 881, 896, 1028
BmiI GGNNCC 4 cut(s) 882, 883, 898, 949
BmrFI CCNGG 3 cut(s) 239, 894, 1032
BmsI GCATC 7 cut(s) 168, 475, 697, 829, 999, 1211, 1236
BpiI GAAGAC 1 cut(s) 57
Bpu14I TTCGAA 1 cut(s) 38
BpuMI CCSGG 1 cut(s) 1032
BsaJI CCNNGG 4 cut(s) 43, 323, 409, 943
Bsc4I CCNNNNNNNGG 1 cut(s) 1037
Bse1I ACTGG 2 cut(s) 621, 1054
Bse3DI GCAATG 2 cut(s) 680, 1012
BseBI CCWGG 2 cut(s) 239, 894
BseDI CCNNGG 4 cut(s) 43, 323, 409, 943
BseGI GGATG 5 cut(s) 461, 480, 841, 853, 1226
BseLI CCNNNNNNNGG 1 cut(s) 1037
BseMI GCAATG 2 cut(s) 680, 1012
BseMII CTCAG 1 cut(s) 477
BseNI ACTGG 2 cut(s) 621, 1054
BseRI GAGGAG 1 cut(s) 568
BseXI GCAGC 5 cut(s) 106, 519, 650, 924, 946
Bsh1236I CGCG 2 cut(s) 45, 81
BshFI GGCC 3 cut(s) 5, 311, 350
BshNI GGYRCC 1 cut(s) 947
BsiHKAI GWGCWC 2 cut(s) 150, 967
BsiHKCI CYCGRG 2 cut(s) 877, 1088
BsiSI CCGG 1 cut(s) 1031
BslFI GGGAC 3 cut(s) 84, 882, 894
BslI CCNNNNNNNGG 1 cut(s) 1037
BsmFI GGGAC 3 cut(s) 84, 882, 894
BsnI GGCC 3 cut(s) 5, 311, 350
BsoBI CYCGRG 2 cut(s) 877, 1088
Bsp119I TTCGAA 1 cut(s) 38
Bsp1286I GDGCHC 2 cut(s) 150, 967
Bsp143I GATC 6 cut(s) 243, 459, 466, 658, 820, 1270
Bsp19I CCATGG 1 cut(s) 323
BspANI GGCC 3 cut(s) 5, 311, 350
BspCNI CTCAG 1 cut(s) 476
BspFNI CGCG 2 cut(s) 45, 81
BspLI GGNNCC 4 cut(s) 882, 883, 898, 949
BspPI GGATC 3 cut(s) 238, 467, 653
BspT104I TTCGAA 1 cut(s) 38
BspT107I GGYRCC 1 cut(s) 947
BsrDI GCAATG 2 cut(s) 680, 1012
BsrI ACTGG 2 cut(s) 621, 1054
BssECI CCNNGG 4 cut(s) 43, 323, 409, 943
BssMI GATC 6 cut(s) 243, 459, 466, 658, 820, 1270
BssT1I CCWWGG 3 cut(s) 323, 409, 943
Bst2UI CCWGG 2 cut(s) 239, 894
Bst4CI ACNGT 3 cut(s) 608, 750, 962
Bst6I CTCTTC 1 cut(s) 122
BstAPI GCANNNNNTGC 2 cut(s) 485, 751
BstBI TTCGAA 1 cut(s) 38
BstC8I GCNNGC 5 cut(s) 150, 287, 668, 973, 1210
BstDEI CTNAG 4 cut(s) 333, 463, 470, 727
BstDSI CCRYGG 2 cut(s) 43, 323
BstF5I GGATG 5 cut(s) 461, 480, 841, 853, 1226
BstFNI CGCG 2 cut(s) 45, 81
BstH2I RGCGCY 1 cut(s) 22
BstHHI GCGC 2 cut(s) 21, 1115
BstKTI GATC 6 cut(s) 246, 462, 469, 661, 823, 1273
BstMBI GATC 6 cut(s) 243, 459, 466, 658, 820, 1270
BstMWI GCNNNNNNNGC 6 cut(s) 149, 176, 485, 524, 751, 799
BstNI CCWGG 2 cut(s) 239, 894
BstSCI CCNGG 3 cut(s) 237, 892, 1030
BstSFI CTRYAG 1 cut(s) 132
BstUI CGCG 2 cut(s) 45, 81
BstV1I GCAGC 5 cut(s) 106, 519, 650, 924, 946
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 4 cut(s) 243, 459, 466, 820
BstYI RGATCY 4 cut(s) 243, 459, 466, 820
BsuRI GGCC 3 cut(s) 5, 311, 350
BtgI CCRYGG 2 cut(s) 43, 323
BtsCI GGATG 5 cut(s) 461, 480, 841, 853, 1226
BtsIMutI CAGTG 3 cut(s) 249, 967, 1047
Cac8I GCNNGC 5 cut(s) 150, 287, 668, 973, 1210
CfoI GCGC 2 cut(s) 21, 1115
Cfr13I GGNCC 5 cut(s) 773, 812, 881, 896, 1028
Cfr42I CCGCGG 1 cut(s) 46
CseI GACGC 1 cut(s) 92
Csp6I GTAC 2 cut(s) 614, 948
CviAII CATG 5 cut(s) 324, 656, 716, 803, 931
CviQI GTAC 2 cut(s) 614, 948
DdeI CTNAG 4 cut(s) 333, 463, 470, 727
DpnI GATC 6 cut(s) 245, 461, 468, 660, 822, 1272
DpnII GATC 6 cut(s) 243, 459, 466, 658, 820, 1270
DraIII CACNNNGTG 1 cut(s) 1162
EaeI YGGCCR 2 cut(s) 3, 348
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
EciI GGCGGA 1 cut(s) 765
Eco130I CCWWGG 3 cut(s) 323, 409, 943
Eco47I GGWCC 5 cut(s) 773, 812, 881, 896, 1028
Eco57I CTGAAG 2 cut(s) 809, 932
Eco88I CYCGRG 2 cut(s) 877, 1088
EcoO109I RGGNCCY 1 cut(s) 881
EcoRI GAATTC 1 cut(s) 328
EcoRII CCWGG 2 cut(s) 237, 892
EcoT14I CCWWGG 3 cut(s) 323, 409, 943
EcoT22I ATGCAT 1 cut(s) 712
ErhI CCWWGG 3 cut(s) 323, 409, 943
FaeI CATG 5 cut(s) 327, 659, 719, 806, 934
FaqI GGGAC 3 cut(s) 84, 882, 894
FatI CATG 5 cut(s) 323, 655, 715, 802, 930
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 8 cut(s) 46, 95, 533, 639, 935, 938, 1196, 1225
FokI GGATG 5 cut(s) 468, 487, 848, 860, 1233
Fsp4HI GCNGC 8 cut(s) 46, 95, 533, 639, 935, 938, 1196, 1225
FspBI CTAG 3 cut(s) 501, 968, 1199
GlaI GCGC 2 cut(s) 20, 1114
GluI GCNGC 8 cut(s) 46, 95, 533, 639, 935, 938, 1196, 1225
HaeII RGCGCY 1 cut(s) 22
HaeIII GGCC 3 cut(s) 5, 311, 350
HapII CCGG 1 cut(s) 1031
HgaI GACGC 1 cut(s) 92
HhaI GCGC 2 cut(s) 21, 1115
Hin1II CATG 5 cut(s) 327, 659, 719, 806, 934
Hin6I GCGC 2 cut(s) 19, 1113
HinP1I GCGC 2 cut(s) 19, 1113
HindIII AAGCTT 1 cut(s) 664
HinfI GANTC 5 cut(s) 24, 213, 620, 852, 919
HpaII CCGG 1 cut(s) 1031
HphI GGTGA 3 cut(s) 97, 716, 1167
Hpy166II GTNNAC 3 cut(s) 202, 372, 580
Hpy188I TCNGA 7 cut(s) 174, 271, 466, 787, 828, 912, 1138
Hpy188III TCNNGA 5 cut(s) 69, 217, 428, 512, 879
Hpy8I GTNNAC 3 cut(s) 202, 372, 580
Hpy99I CGWCG 3 cut(s) 86, 178, 190
HpyAV CCTTC 4 cut(s) 75, 118, 425, 1047
HpyCH4III ACNGT 3 cut(s) 608, 750, 962
HpyCH4IV ACGT 1 cut(s) 866
HpyCH4V TGCA 8 cut(s) 588, 655, 685, 710, 754, 905, 934, 975
HpyF10VI GCNNNNNNNGC 6 cut(s) 149, 176, 485, 524, 751, 799
HpyF3I CTNAG 4 cut(s) 333, 463, 470, 727
HpySE526I ACGT 1 cut(s) 866
Hsp92II CATG 5 cut(s) 327, 659, 719, 806, 934
HspAI GCGC 2 cut(s) 19, 1113
KflI GGGWCCC 1 cut(s) 881
KpnI GGTACC 1 cut(s) 951
KspI CCGCGG 1 cut(s) 46
Kzo9I GATC 6 cut(s) 243, 459, 466, 658, 820, 1270
LmnI GCTCC 3 cut(s) 175, 798, 945
Lsp1109I GCAGC 5 cut(s) 106, 519, 650, 924, 946
LweI GCATC 7 cut(s) 168, 475, 697, 829, 999, 1211, 1236
MaeI CTAG 3 cut(s) 501, 968, 1199
MaeII ACGT 1 cut(s) 866
MaeIII GTNAC 1 cut(s) 103
MalI GATC 6 cut(s) 245, 461, 468, 660, 822, 1272
MboI GATC 6 cut(s) 243, 459, 466, 658, 820, 1270
MboII GAAGA 6 cut(s) 62, 109, 112, 556, 815, 1265
MflI RGATCY 4 cut(s) 243, 459, 466, 820
MhlI GDGCHC 2 cut(s) 150, 967
MlsI TGGCCA 2 cut(s) 5, 350
MluNI TGGCCA 2 cut(s) 5, 350
MlyI GAGTC 1 cut(s) 33
MmeI TCCRAC 4 cut(s) 197, 820, 1053, 1074
MnlI CCTC 9 cut(s) 125, 203, 257, 546, 726, 918, 1019, 1028, 1195
Mox20I TGGCCA 2 cut(s) 5, 350
Mph1103I ATGCAT 1 cut(s) 712
MroXI GAANNNNTTC 2 cut(s) 265, 519
MscI TGGCCA 2 cut(s) 5, 350
MseI TTAA 2 cut(s) 422, 567
Msp20I TGGCCA 2 cut(s) 5, 350
MspA1I CMGCKG 2 cut(s) 45, 937
MspI CCGG 1 cut(s) 1031
MspR9I CCNGG 3 cut(s) 239, 894, 1032
MvaI CCWGG 2 cut(s) 239, 894
MvnI CGCG 2 cut(s) 45, 81
MwoI GCNNNNNNNGC 6 cut(s) 149, 176, 485, 524, 751, 799
NciI CCSGG 1 cut(s) 1032
NcoI CCATGG 1 cut(s) 323
NdeII GATC 6 cut(s) 243, 459, 466, 658, 820, 1270
NlaIII CATG 5 cut(s) 327, 659, 719, 806, 934
NlaIV GGNNCC 4 cut(s) 882, 883, 898, 949
NmeAIII GCCGAG 1 cut(s) 47
NmuCI GTSAC 1 cut(s) 103
NsiI ATGCAT 1 cut(s) 712
NspV TTCGAA 1 cut(s) 38
PcsI WCGNNNNNNNCGW 3 cut(s) 182, 201, 863
PdmI GAANNNNTTC 2 cut(s) 265, 519
PfeI GAWTC 4 cut(s) 213, 620, 852, 919
PkrI GCNGC 8 cut(s) 47, 96, 534, 640, 936, 939, 1197, 1226
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
PpuMI RGGWCCY 1 cut(s) 881
PshBI ATTAAT 1 cut(s) 567
PsiI TTATAA 1 cut(s) 692
Psp5II RGGWCCY 1 cut(s) 881
Psp6I CCWGG 2 cut(s) 237, 892
PspGI CCWGG 2 cut(s) 237, 892
PspN4I GGNNCC 4 cut(s) 882, 883, 898, 949
PspPI GGNCC 5 cut(s) 773, 812, 881, 896, 1028
PspPPI RGGWCCY 1 cut(s) 881
PsuI RGATCY 4 cut(s) 243, 459, 466, 820
PvuII CAGCTG 1 cut(s) 937
RsaI GTAC 2 cut(s) 615, 949
RsaNI GTAC 2 cut(s) 614, 948
SacII CCGCGG 1 cut(s) 46
SaqAI TTAA 2 cut(s) 422, 567
SatI GCNGC 8 cut(s) 46, 95, 533, 639, 935, 938, 1196, 1225
Sau3AI GATC 6 cut(s) 243, 459, 466, 658, 820, 1270
Sau96I GGNCC 5 cut(s) 773, 812, 881, 896, 1028
SchI GAGTC 1 cut(s) 33
ScrFI CCNGG 3 cut(s) 239, 894, 1032
SduI GDGCHC 2 cut(s) 150, 967
SfaNI GCATC 7 cut(s) 168, 475, 697, 829, 999, 1211, 1236
SfcI CTRYAG 1 cut(s) 132
Sfr303I CCGCGG 1 cut(s) 46
SfuI TTCGAA 1 cut(s) 38
SgrBI CCGCGG 1 cut(s) 46
SinI GGWCC 5 cut(s) 773, 812, 881, 896, 1028
SspMI CTAG 3 cut(s) 501, 968, 1199
StyD4I CCNGG 3 cut(s) 237, 892, 1030
StyI CCWWGG 3 cut(s) 323, 409, 943
TaaI ACNGT 3 cut(s) 608, 750, 962
TaiI ACGT 1 cut(s) 869
TaqI TCGA 2 cut(s) 38, 1269
TaqII GACCGA 1 cut(s) 69
TatI WGTACW 1 cut(s) 613
TauI GCSGC 3 cut(s) 48, 1198, 1227
TfiI GAWTC 4 cut(s) 213, 620, 852, 919
Tru1I TTAA 2 cut(s) 422, 567
Tru9I TTAA 2 cut(s) 422, 567
TscAI CASTG 3 cut(s) 256, 967, 1054
TseFI GTSAC 1 cut(s) 103
TseI GCWGC 5 cut(s) 94, 532, 638, 934, 937
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 2 cut(s) 911, 1158
TspRI CASTG 3 cut(s) 256, 967, 1054
VpaK11BI GGWCC 5 cut(s) 773, 812, 881, 896, 1028
VspI ATTAAT 1 cut(s) 567
XapI RAATTY 3 cut(s) 328, 648, 673
XmiI GTMKAC 1 cut(s) 201
XmnI GAANNNNTTC 2 cut(s) 265, 519
XspI CTAG 3 cut(s) 501, 968, 1199
Zsp2I ATGCAT 1 cut(s) 712
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.