Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0002737

Physical Location & Seq

Genomic Coordinates Reverse (-)

26150014 .. 26153060

Gene Structure 3047 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1153200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 906 bp

ATGGCCGAGTTGACTCAGGGCGAGGTGGTCTACTCGCCCAAGACTCTCCAAGTCTGGCGAGCTCTGCTCAACTGGCTCGGCTTCTTCTTCCAGATCTTCCTCCGAATCCTCCGAGCTCTAGGCCACCACCCCCTCCTCTCTTCCTCCTCCTCCTCTTCCGCTTCTTCCGCCGCCTTCAAATCACTGCCCGTCATCGAGCTGCCCGAGAACGACTCCCCCGCCGCCTCCGCCGTTGTAATCGCCGACACTCCCGATTCCGACTCCGACGACCCTTTTGAAAAGCTCACGGTAGTTCTGGACCTGGATGAAACCCTAGTGTGTGCATATGAAACATCCAGCTTGCCGGCTGTAGTTCGAACTCAAGCAACTGAAGGTGGGATGAAGTGGTTTGAGCTTGAATGCGTATCTTCGGACAAGGAATGTGATGGCAAACCGAAGGTCAATTATGTCACGGTCTTTGAGCGTCCAGGGTTGCATGACTTCCTAAAACAAGTTAGTCAGTTTGCAGAGCTTGTGCTATTCACTGCTGGTCTTGAAGGTTATGCTAGGCCACTTGTTGACAGAATAGATGTGGATAATCTGTTCAGTTGTCGACTTTATCGGCCTTCAACAACTAGCACGGAATACCGGGAGCATGTGAAGGATCTATCTGGTCTATCAAAGGATATGCGCCGAATTGTTATTGTTGACAACAATCCTTTCAGTTTCTTGTTGCAACCTTCGAATGGAATTCCATGTATTCCATTTTCTGCCGGGCAAACACACGACACACAGCTTTTGGATGTTCTTCTTCCACTCCTCAAGCAGCTCTCGCTGCAGAAAGATGTGAGACCTGCCCTTTATGAAAGGTTTCACATGCCCGAATGGTTTCAAAAGCAGGGGATTCCTTCAAATGCTTGGAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0036211 GO:0042578 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NIF

Protein Analysis

302

Amino Acids

33.73

Weight (kDa)

5.12

Isoelectric Point (pI)

40.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NIF	PF03031	95 - 277	1e-42	NLI interacting factor-like phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002737)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55960
fragaria_vesca	FvH4_6g11400 FvH4_6g11400 FvH4_7g24520
malus_domestica	MD01G1153200.v1.1 MD04G1153700.v1.1 MD07G1222200.v1.1 MD12G1166900.v1.1
prunus_persica	Prupe.2G250400_v2.0.a1 Prupe.6G277800_v2.0.a1
pyrus_communis	pycom01g17080 pycom07g20080 pycom12g15890
rosa_chinensis	RchiOBHm_Chr1g0370271 RchiOBHm_Chr1g0370291 RchiOBHm_Chr3g0463021
rosa_laevigata	RLG00000024816 RLG00000027058 RLG00000027059 RLG00000027062
rosa_multiflora	Rmu_sc0000766.1_g000029 Rmu_sc0001657.1_g000001 Rmu_sc0006707.1_g000006 Rmu_sc0008124.1_g000028
rosa_roxburghii	Rroxscaffold_4G00286680 Rroxscaffold_4G00287340 Rroxscaffold_6G00417040
rosa_rugosa	Rorug01G0355600 Rorug03G0061100 Rorug03G0061200 Rorug03G0061200
rosa_samantha	Rh1AG363900 Rh1BG327000 Rh1BG327100 Rh1DG357400 Rh3AG118800 Rh3DG124700
rosa_wichuraiana	Rw1G032030 Rw3G010090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	841
AccI	GTMKAC	2 cut(s)	30, 592
AciI	CCGC	6 cut(s)	159, 168, 171, 219, 222, 228
AclWI	GGATC	1 cut(s)	651
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	1 cut(s)	729
AcuI	CTGAAG	1 cut(s)	390
AfiI	CCNNNNNNNGG	1 cut(s)	725
AgsI	TTSAA	7 cut(s)	178, 278, 398, 536, 609, 872, 891
AjnI	CCWGG	2 cut(s)	300, 466
AloI	GAACNNNNNNTCC	2 cut(s)	566, 598
AluBI	AGCT	9 cut(s)	62, 116, 199, 283, 339, 394, 511, 775, 808
AluI	AGCT	9 cut(s)	62, 116, 199, 283, 339, 394, 511, 775, 808
Alw21I	GWGCWC	2 cut(s)	64, 118
Alw26I	GTCTC	1 cut(s)	823
AlwI	GGATC	1 cut(s)	651
Ama87I	CYCGRG	1 cut(s)	203
AoxI	GGCC	4 cut(s)	3, 121, 548, 602
ApeKI	GCWGC	3 cut(s)	199, 805, 814
ApoI	RAATTY	1 cut(s)	729
ArsI	GACNNNNNNTTYG	4 cut(s)	257, 289, 423, 455
Asp700I	GAANNNNTTC	2 cut(s)	849, 867
AspLEI	GCGC	1 cut(s)	672
AspS9I	GGNCC	1 cut(s)	298
AsuC2I	CCSGG	2 cut(s)	629, 754
AsuII	TTCGAA	2 cut(s)	355, 722
AvaI	CYCGRG	1 cut(s)	203
AvaII	GGWCC	1 cut(s)	298
BanII	GRGCYC	2 cut(s)	64, 118
Bbv12I	GWGCWC	2 cut(s)	64, 118
BbvI	GCAGC	3 cut(s)	186, 801, 817
BccI	CCATC	1 cut(s)	419
BceAI	ACGGC	1 cut(s)	215
BciT130I	CCWGG	2 cut(s)	302, 468
BcnI	CCSGG	2 cut(s)	629, 754
BcoDI	GTCTC	1 cut(s)	823
BfaI	CTAG	4 cut(s)	119, 314, 546, 615
BfmI	CTRYAG	2 cut(s)	348, 815
BfuAI	ACCTGC	1 cut(s)	841
BglII	AGATCT	1 cut(s)	93
BisI	GCNGC	5 cut(s)	171, 200, 222, 806, 815
BlsI	GCNGC	5 cut(s)	172, 201, 223, 807, 816
Bme1390I	CCNGG	4 cut(s)	302, 468, 629, 754
Bme18I	GGWCC	1 cut(s)	298
BmeT110I	CYCGRG	1 cut(s)	203
BmgT120I	GGNCC	1 cut(s)	298
BmrFI	CCNGG	4 cut(s)	302, 468, 629, 754
BplI	GAGNNNNNCTC	4 cut(s)	51, 83, 197, 229
Bpu14I	TTCGAA	2 cut(s)	355, 722
BpuEI	CTTGAG	2 cut(s)	345, 785
BpuMI	CCSGG	2 cut(s)	629, 754
BsaI	GGTCTC	1 cut(s)	823
BsaJI	CCNNGG	1 cut(s)	467
Bsc4I	CCNNNNNNNGG	1 cut(s)	725
Bse118I	RCCGGY	1 cut(s)	343
Bse1I	ACTGG	1 cut(s)	77
BseBI	CCWGG	2 cut(s)	302, 468
BseDI	CCNNGG	1 cut(s)	467
BseGI	GGATG	4 cut(s)	310, 332, 384, 787
BseLI	CCNNNNNNNGG	1 cut(s)	725
BseMII	CTCAG	1 cut(s)	29
BseNI	ACTGG	1 cut(s)	77
BseRI	GAGGAG	5 cut(s)	125, 136, 139, 142, 788
BseXI	GCAGC	3 cut(s)	186, 801, 817
BshFI	GGCC	4 cut(s)	5, 123, 550, 604
BsiHKAI	GWGCWC	2 cut(s)	64, 118
BsiHKCI	CYCGRG	1 cut(s)	203
BsiSI	CCGG	3 cut(s)	344, 628, 753
BslI	CCNNNNNNNGG	1 cut(s)	725
BsmAI	GTCTC	1 cut(s)	823
BsmI	GAATGC	1 cut(s)	404
BsnI	GGCC	4 cut(s)	5, 123, 550, 604
Bso31I	GGTCTC	1 cut(s)	823
BsoBI	CYCGRG	1 cut(s)	203
Bsp119I	TTCGAA	2 cut(s)	355, 722
Bsp1286I	GDGCHC	2 cut(s)	64, 118
Bsp143I	GATC	2 cut(s)	93, 643
BspACI	CCGC	6 cut(s)	159, 168, 171, 219, 222, 228
BspANI	GGCC	4 cut(s)	5, 123, 550, 604
BspCNI	CTCAG	1 cut(s)	28
BspMAI	CTGCAG	1 cut(s)	819
BspMI	ACCTGC	1 cut(s)	841
BspPI	GGATC	1 cut(s)	651
BspT104I	TTCGAA	2 cut(s)	355, 722
BspTNI	GGTCTC	1 cut(s)	823
BsrFI	RCCGGY	1 cut(s)	343
BsrI	ACTGG	1 cut(s)	77
BssAI	RCCGGY	1 cut(s)	343
BssECI	CCNNGG	1 cut(s)	467
BssMI	GATC	2 cut(s)	93, 643
Bst2UI	CCWGG	2 cut(s)	302, 468
Bst4CI	ACNGT	2 cut(s)	289, 454
Bst6I	CTCTTC	2 cut(s)	145, 160
BstBI	TTCGAA	2 cut(s)	355, 722
BstC8I	GCNNGC	3 cut(s)	60, 341, 345
BstDEI	CTNAG	1 cut(s)	15
BstF5I	GGATG	4 cut(s)	310, 332, 384, 787
BstHHI	GCGC	1 cut(s)	672
BstKTI	GATC	2 cut(s)	96, 646
BstMAI	GTCTC	1 cut(s)	823
BstMBI	GATC	2 cut(s)	93, 643
BstMWI	GCNNNNNNNGC	6 cut(s)	64, 73, 167, 227, 811, 814
BstNI	CCWGG	2 cut(s)	302, 468
BstNSI	RCATGY	2 cut(s)	638, 859
BstSCI	CCNGG	4 cut(s)	300, 466, 627, 752
BstSFI	CTRYAG	2 cut(s)	348, 815
BstV1I	GCAGC	3 cut(s)	186, 801, 817
BstX2I	RGATCY	2 cut(s)	93, 643
BstYI	RGATCY	2 cut(s)	93, 643
BsuRI	GGCC	4 cut(s)	5, 123, 550, 604
BtsCI	GGATG	4 cut(s)	310, 332, 384, 787
BtsI	GCAGTG	2 cut(s)	182, 522
BtsIMutI	CAGTG	2 cut(s)	182, 522
BveI	ACCTGC	1 cut(s)	841
Cac8I	GCNNGC	3 cut(s)	60, 341, 345
CfoI	GCGC	1 cut(s)	672
Cfr10I	RCCGGY	1 cut(s)	343
Cfr13I	GGNCC	1 cut(s)	298
CseI	GACGC	1 cut(s)	452
CviAII	CATG	4 cut(s)	476, 635, 735, 856
DdeI	CTNAG	1 cut(s)	15
DpnI	GATC	2 cut(s)	95, 645
DpnII	GATC	2 cut(s)	93, 643
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	2 cut(s)	145, 160
EarI	CTCTTC	2 cut(s)	145, 160
EciI	GGCGGA	2 cut(s)	157, 217
Ecl136II	GAGCTC	2 cut(s)	62, 116
Eco24I	GRGCYC	2 cut(s)	64, 118
Eco31I	GGTCTC	1 cut(s)	823
Eco47I	GGWCC	1 cut(s)	298
Eco53kI	GAGCTC	2 cut(s)	62, 116
Eco57I	CTGAAG	1 cut(s)	390
Eco88I	CYCGRG	1 cut(s)	203
EcoICRI	GAGCTC	2 cut(s)	62, 116
EcoRI	GAATTC	1 cut(s)	729
EcoRII	CCWGG	2 cut(s)	300, 466
EcoT38I	GRGCYC	2 cut(s)	64, 118
FaeI	CATG	4 cut(s)	479, 638, 738, 859
FatI	CATG	4 cut(s)	475, 634, 734, 855
FauI	CCCGC	1 cut(s)	226
FauNDI	CATATG	1 cut(s)	325
FblI	GTMKAC	2 cut(s)	30, 592
Fnu4HI	GCNGC	5 cut(s)	171, 200, 222, 806, 815
FokI	GGATG	4 cut(s)	317, 319, 391, 794
FriOI	GRGCYC	2 cut(s)	64, 118
Fsp4HI	GCNGC	5 cut(s)	171, 200, 222, 806, 815
FspBI	CTAG	4 cut(s)	119, 314, 546, 615
GlaI	GCGC	1 cut(s)	671
GluI	GCNGC	5 cut(s)	171, 200, 222, 806, 815
HaeIII	GGCC	4 cut(s)	5, 123, 550, 604
HapII	CCGG	3 cut(s)	344, 628, 753
HgaI	GACGC	1 cut(s)	452
HhaI	GCGC	1 cut(s)	672
Hin1II	CATG	4 cut(s)	479, 638, 738, 859
Hin6I	GCGC	1 cut(s)	670
HinP1I	GCGC	1 cut(s)	670
HincII	GTYRAC	4 cut(s)	12, 559, 593, 688
HindII	GTYRAC	4 cut(s)	12, 559, 593, 688
HinfI	GANTC	7 cut(s)	13, 43, 105, 212, 254, 260, 883
HpaII	CCGG	3 cut(s)	344, 628, 753
Hpy166II	GTNNAC	5 cut(s)	12, 31, 559, 593, 688
Hpy188I	TCNGA	5 cut(s)	104, 113, 259, 265, 412
Hpy188III	TCNNGA	4 cut(s)	91, 251, 296, 533
Hpy8I	GTNNAC	5 cut(s)	12, 31, 559, 593, 688
Hpy99I	CGWCG	1 cut(s)	269
HpyAV	CCTTC	8 cut(s)	184, 365, 430, 530, 615, 634, 729, 897
HpyCH4III	ACNGT	2 cut(s)	289, 454
HpyCH4V	TGCA	5 cut(s)	323, 475, 506, 715, 817
HpyF10VI	GCNNNNNNNGC	6 cut(s)	64, 73, 167, 227, 811, 814
HpyF3I	CTNAG	1 cut(s)	15
Hsp92II	CATG	4 cut(s)	479, 638, 738, 859
HspAI	GCGC	1 cut(s)	670
KroI	GCCGGC	1 cut(s)	343
KroNI	GCCGGC	1 cut(s)	345
Kzo9I	GATC	2 cut(s)	93, 643
LmnI	GCTCC	1 cut(s)	631
Lsp1109I	GCAGC	3 cut(s)	186, 801, 817
MaeI	CTAG	4 cut(s)	119, 314, 546, 615
MaeIII	GTNAC	1 cut(s)	448
MalI	GATC	2 cut(s)	95, 645
MboI	GATC	2 cut(s)	93, 643
MboII	GAAGA	9 cut(s)	76, 79, 88, 132, 147, 156, 399, 779, 782
MflI	RGATCY	2 cut(s)	93, 643
MhlI	GDGCHC	2 cut(s)	64, 118
MluCI	AATT	3 cut(s)	442, 675, 729
MlyI	GAGTC	4 cut(s)	7, 37, 206, 254
MmeI	TCCRAC	2 cut(s)	282, 288
MroNI	GCCGGC	1 cut(s)	343
MroXI	GAANNNNTTC	2 cut(s)	849, 867
MspI	CCGG	3 cut(s)	344, 628, 753
MspR9I	CCNGG	4 cut(s)	302, 468, 629, 754
Mva1269I	GAATGC	1 cut(s)	404
MvaI	CCWGG	2 cut(s)	302, 468
MwoI	GCNNNNNNNGC	6 cut(s)	64, 73, 167, 227, 811, 814
NaeI	GCCGGC	1 cut(s)	345
NciI	CCSGG	2 cut(s)	629, 754
NdeI	CATATG	1 cut(s)	325
NdeII	GATC	2 cut(s)	93, 643
NgoMIV	GCCGGC	1 cut(s)	343
NlaIII	CATG	4 cut(s)	479, 638, 738, 859
NmeAIII	GCCGAG	2 cut(s)	31, 57
NmuCI	GTSAC	1 cut(s)	448
NspI	RCATGY	2 cut(s)	638, 859
NspV	TTCGAA	2 cut(s)	355, 722
PcsI	WCGNNNNNNNCGW	1 cut(s)	201
PctI	GAATGC	1 cut(s)	404
PdiI	GCCGGC	1 cut(s)	345
PdmI	GAANNNNTTC	2 cut(s)	849, 867
PfeI	GAWTC	3 cut(s)	105, 254, 883
PkrI	GCNGC	5 cut(s)	172, 201, 223, 807, 816
PleI	GAGTC	4 cut(s)	7, 37, 206, 254
PpsI	GAGTC	4 cut(s)	7, 37, 206, 254
Psp124BI	GAGCTC	2 cut(s)	64, 118
Psp6I	CCWGG	2 cut(s)	300, 466
PspGI	CCWGG	2 cut(s)	300, 466
PspPI	GGNCC	1 cut(s)	298
PstI	CTGCAG	1 cut(s)	819
PsuI	RGATCY	2 cut(s)	93, 643
SacI	GAGCTC	2 cut(s)	64, 118
SalI	GTCGAC	1 cut(s)	591
SatI	GCNGC	5 cut(s)	171, 200, 222, 806, 815
Sau3AI	GATC	2 cut(s)	93, 643
Sau96I	GGNCC	1 cut(s)	298
SchI	GAGTC	4 cut(s)	7, 37, 206, 254
ScrFI	CCNGG	4 cut(s)	302, 468, 629, 754
SduI	GDGCHC	2 cut(s)	64, 118
SfcI	CTRYAG	2 cut(s)	348, 815
SfuI	TTCGAA	2 cut(s)	355, 722
SinI	GGWCC	1 cut(s)	298
SmlI	CTYRAG	2 cut(s)	360, 800
SmoI	CTYRAG	2 cut(s)	360, 800
Sse9I	AATT	3 cut(s)	442, 675, 729
SsiI	CCGC	6 cut(s)	159, 168, 171, 219, 222, 228
SspMI	CTAG	4 cut(s)	119, 314, 546, 615
SstI	GAGCTC	2 cut(s)	64, 118
StyD4I	CCNGG	4 cut(s)	300, 466, 627, 752
TaaI	ACNGT	2 cut(s)	289, 454
TaqI	TCGA	4 cut(s)	195, 355, 592, 722
TasI	AATT	3 cut(s)	442, 675, 729
TauI	GCSGC	2 cut(s)	173, 224
TfiI	GAWTC	3 cut(s)	105, 254, 883
TscAI	CASTG	2 cut(s)	189, 529
TseFI	GTSAC	1 cut(s)	448
TseI	GCWGC	3 cut(s)	199, 805, 814
Tsp45I	GTSAC	1 cut(s)	448
TspDTI	ATGAA	4 cut(s)	321, 342, 395, 858
TspGWI	ACGGA	1 cut(s)	635
TspRI	CASTG	2 cut(s)	189, 529
VpaK11BI	GGWCC	1 cut(s)	298
XapI	RAATTY	1 cut(s)	729
XceI	RCATGY	2 cut(s)	638, 859
XmiI	GTMKAC	2 cut(s)	30, 592
XmnI	GAANNNNTTC	2 cut(s)	849, 867
XspI	CTAG	4 cut(s)	119, 314, 546, 615

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.