Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002737

Physical Location & Seq

Genomic Coordinates Reverse (-)

24195496 .. 24198148

Gene Structure 2653 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1153700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 894 bp

ATGGCGCCGATGGGACAGCCTGCCGCCGCCGCCGAAGTGTACGTGCCGATGACCCTTCAAGTGTGGCGAGGTCTCGTGAACTGGGTGGGCTTCTTCTTCCAGATTATTCTTCAGATCCTGAGAGGCGCGCCTTGCCTTCCTCACCTGCTGTCCTGCATTTCTTCCTCTTCTCCTCCTGCTTTCAGGCCTCTGCCCGTCGCCGAAGTCCCTCTCAACGACTTGTCGTCCTCCGCCCACGTCGAAAACGGCGTCGTTGATGGTCGCCCCGTCGAAAAGCTCACGGTAGTTCTTGATTTGGATGAGACTCTAGTATGTGCTTATGAAACATGTAGCGTGCCTGCGATTGTTCGTGCTCAAGCAACGGAAGCTGGGCTGAAGTGGTTCGAAATAGAATGTCTTTCTTCAGGGAAGGAGTCTGATGGTAAACCAAAGGTCAATTATGTGACAGTGTTTGAACGTCCTGGTTTGCGAGAGTTCTTGAAGCGGGTTTGTGAGTTTGCTGATCTTGTGCTATTCACTGCTGGTCTTGAAGATTATGCTAGACCACTTGTTGACAGAATTGATGTGGAGAATCTGTTTAAGCTTCGTCTTTATCGGCCTTCTACGGTTAGCACGGAATTTCGGGAGCATGTGAAAGATCTATCATGTTTGTCAAAAGATTTCTCTCGAATCGTCATTGTTGACAACAACCCATTCAGTTTTTTGCTGCAACCGTTGAATGGAATTCCATGCGTTCCATTTTCTGCTGGACAGCCATACGATGATCAGCTTTTGGAGGTCCTGCATCCTCTCCTCATGCAGCTCTCCTTGCAAAAAGATGTGAGACCTATGCTTCATGAGAAATTTCAAATGCCTGAATGGTTTCAAATGCATGGATTCCCCGTTTCTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0036211 GO:0042578 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NIF

Protein Analysis

298

Amino Acids

33.33

Weight (kDa)

5.14

Isoelectric Point (pI)

44.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NIF	PF03031	93 - 275	9.5e-40	NLI interacting factor-like phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002737)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55960
fragaria_vesca	FvH4_6g11400 FvH4_6g11400 FvH4_7g24520
malus_domestica	MD01G1153200.v1.1 MD04G1153700.v1.1 MD07G1222200.v1.1 MD12G1166900.v1.1
prunus_persica	Prupe.2G250400_v2.0.a1 Prupe.6G277800_v2.0.a1
pyrus_communis	pycom01g17080 pycom07g20080 pycom12g15890
rosa_chinensis	RchiOBHm_Chr1g0370271 RchiOBHm_Chr1g0370291 RchiOBHm_Chr3g0463021
rosa_laevigata	RLG00000024816 RLG00000027058 RLG00000027059 RLG00000027062
rosa_multiflora	Rmu_sc0000766.1_g000029 Rmu_sc0001657.1_g000001 Rmu_sc0006707.1_g000006 Rmu_sc0008124.1_g000028
rosa_roxburghii	Rroxscaffold_4G00286680 Rroxscaffold_4G00287340 Rroxscaffold_6G00417040
rosa_rugosa	Rorug01G0355600 Rorug03G0061100 Rorug03G0061200 Rorug03G0061200
rosa_samantha	Rh1AG363900 Rh1BG327000 Rh1BG327100 Rh1DG357400 Rh3AG118800 Rh3DG124700
rosa_wichuraiana	Rw1G032030 Rw3G010090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	153
Acc36I	ACCTGC	1 cut(s)	153
AccB1I	GGYRCC	1 cut(s)	4
AccII	CGCG	1 cut(s)	128
AciI	CCGC	5 cut(s)	24, 27, 30, 231, 484
AclWI	GGATC	1 cut(s)	109
AcsI	RAATTY	3 cut(s)	617, 723, 842
AcuI	CTGAAG	3 cut(s)	95, 387, 395
AcyI	GRCGYC	2 cut(s)	5, 249
AfaI	GTAC	1 cut(s)	41
AfiI	CCNNNNNNNGG	1 cut(s)	719
AflIII	ACRYGT	1 cut(s)	326
AgsI	TTSAA	7 cut(s)	59, 455, 481, 530, 718, 848, 866
AhdI	GACNNNNNGTC	1 cut(s)	223
AjiI	CACGTC	1 cut(s)	238
AjnI	CCWGG	1 cut(s)	460
AluBI	AGCT	5 cut(s)	277, 368, 583, 769, 802
AluI	AGCT	5 cut(s)	277, 368, 583, 769, 802
Alw21I	GWGCWC	1 cut(s)	355
Alw26I	GTCTC	3 cut(s)	77, 296, 817
AlwI	GGATC	1 cut(s)	109
AlwNI	CAGNNNCTG	1 cut(s)	118
AoxI	GGCC	2 cut(s)	185, 596
ApeKI	GCWGC	2 cut(s)	706, 799
ApoI	RAATTY	3 cut(s)	617, 723, 842
ArsI	GACNNNNNNTTYG	2 cut(s)	234, 266
AscI	GGCGCGCC	1 cut(s)	126
Asp700I	GAANNNNTTC	2 cut(s)	380, 861
AspLEI	GCGC	3 cut(s)	7, 128, 130
AspS9I	GGNCC	1 cut(s)	778
AsuHPI	GGTGA	1 cut(s)	134
AsuII	TTCGAA	1 cut(s)	384
AvaII	GGWCC	1 cut(s)	778
BanI	GGYRCC	1 cut(s)	4
BauI	CACGAG	1 cut(s)	74
Bbv12I	GWGCWC	1 cut(s)	355
BbvI	GCAGC	2 cut(s)	693, 811
BccI	CCATC	3 cut(s)	4, 251, 413
BceAI	ACGGC	1 cut(s)	262
BciT130I	CCWGG	1 cut(s)	462
BclI	TGATCA	1 cut(s)	763
BcoDI	GTCTC	3 cut(s)	77, 296, 817
BfaI	CTAG	2 cut(s)	308, 540
BfoI	RGCGCY	1 cut(s)	8
BfuAI	ACCTGC	1 cut(s)	153
BglII	AGATCT	1 cut(s)	637
BisI	GCNGC	5 cut(s)	24, 27, 30, 707, 800
BlsI	GCNGC	5 cut(s)	25, 28, 31, 708, 801
Bme1390I	CCNGG	1 cut(s)	462
Bme18I	GGWCC	1 cut(s)	778
BmeRI	GACNNNNNGTC	1 cut(s)	223
BmgBI	CACGTC	1 cut(s)	238
BmgT120I	GGNCC	1 cut(s)	778
BmiI	GGNNCC	1 cut(s)	6
BmrFI	CCNGG	1 cut(s)	462
BmrI	ACTGGG	1 cut(s)	91
BmsI	GCATC	1 cut(s)	793
BmuI	ACTGGG	1 cut(s)	91
Bpu14I	TTCGAA	1 cut(s)	384
BpuEI	CTTGAG	1 cut(s)	339
BsaAI	YACGTR	1 cut(s)	43
BsaHI	GRCGYC	2 cut(s)	5, 249
BsaI	GGTCTC	2 cut(s)	77, 817
BsaXI	ACNNNNNCTCC	2 cut(s)	560, 590
Bsc4I	CCNNNNNNNGG	1 cut(s)	719
Bse1I	ACTGG	1 cut(s)	86
BseBI	CCWGG	1 cut(s)	462
BseGI	GGATG	2 cut(s)	304, 784
BseLI	CCNNNNNNNGG	1 cut(s)	719
BseMII	CTCAG	1 cut(s)	110
BseNI	ACTGG	1 cut(s)	86
BsePI	GCGCGC	1 cut(s)	126
BseRI	GAGGAG	2 cut(s)	162, 782
BseXI	GCAGC	2 cut(s)	693, 811
BseYI	CCCAGC	1 cut(s)	368
Bsh1236I	CGCG	1 cut(s)	128
BshFI	GGCC	2 cut(s)	187, 598
BshNI	GGYRCC	1 cut(s)	4
BsiHKAI	GWGCWC	1 cut(s)	355
BslFI	GGGAC	2 cut(s)	27, 191
BslI	CCNNNNNNNGG	1 cut(s)	719
BsmAI	GTCTC	3 cut(s)	77, 296, 817
BsmFI	GGGAC	2 cut(s)	27, 191
BsnI	GGCC	2 cut(s)	187, 598
Bso31I	GGTCTC	2 cut(s)	77, 817
Bsp119I	TTCGAA	1 cut(s)	384
Bsp1286I	GDGCHC	1 cut(s)	355
Bsp143I	GATC	4 cut(s)	114, 502, 637, 763
BspACI	CCGC	5 cut(s)	24, 27, 30, 231, 484
BspANI	GGCC	2 cut(s)	187, 598
BspCNI	CTCAG	1 cut(s)	111
BspFNI	CGCG	1 cut(s)	128
BspHI	TCATGA	1 cut(s)	835
BspLI	GGNNCC	1 cut(s)	6
BspMI	ACCTGC	1 cut(s)	153
BspPI	GGATC	1 cut(s)	109
BspT104I	TTCGAA	1 cut(s)	384
BspT107I	GGYRCC	1 cut(s)	4
BspTNI	GGTCTC	2 cut(s)	77, 817
BsrI	ACTGG	1 cut(s)	86
BssHII	GCGCGC	1 cut(s)	126
BssMI	GATC	4 cut(s)	114, 502, 637, 763
BssNI	GRCGYC	2 cut(s)	5, 249
BssSI	CACGAG	1 cut(s)	74
Bst2BI	CACGAG	1 cut(s)	74
Bst2UI	CCWGG	1 cut(s)	462
Bst4CI	ACNGT	4 cut(s)	283, 448, 607, 714
Bst6I	CTCTTC	1 cut(s)	172
BstACI	GRCGYC	2 cut(s)	5, 249
BstBAI	YACGTR	1 cut(s)	43
BstBI	TTCGAA	1 cut(s)	384
BstC8I	GCNNGC	4 cut(s)	21, 128, 335, 339
BstDEI	CTNAG	1 cut(s)	119
BstF5I	GGATG	2 cut(s)	304, 784
BstFNI	CGCG	1 cut(s)	128
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	3 cut(s)	7, 128, 130
BstKTI	GATC	4 cut(s)	117, 505, 640, 766
BstMAI	GTCTC	3 cut(s)	77, 296, 817
BstMBI	GATC	4 cut(s)	114, 502, 637, 763
BstMWI	GCNNNNNNNGC	4 cut(s)	29, 132, 365, 808
BstNI	CCWGG	1 cut(s)	462
BstNSI	RCATGY	2 cut(s)	330, 632
BstSCI	CCNGG	1 cut(s)	460
BstUI	CGCG	1 cut(s)	128
BstV1I	GCAGC	2 cut(s)	693, 811
BstX2I	RGATCY	2 cut(s)	114, 637
BstYI	RGATCY	2 cut(s)	114, 637
BsuRI	GGCC	2 cut(s)	187, 598
BtrI	CACGTC	1 cut(s)	238
BtsCI	GGATG	2 cut(s)	304, 784
BtsI	GCAGTG	1 cut(s)	516
BtsIMutI	CAGTG	2 cut(s)	453, 516
BveI	ACCTGC	1 cut(s)	153
Cac8I	GCNNGC	4 cut(s)	21, 128, 335, 339
CaiI	CAGNNNCTG	1 cut(s)	118
CciI	TCATGA	1 cut(s)	835
CfoI	GCGC	3 cut(s)	7, 128, 130
Cfr13I	GGNCC	1 cut(s)	778
CseI	GACGC	1 cut(s)	238
Csp6I	GTAC	1 cut(s)	40
CviAII	CATG	7 cut(s)	327, 629, 645, 729, 796, 836, 872
CviQI	GTAC	1 cut(s)	40
DdeI	CTNAG	1 cut(s)	119
DinI	GGCGCC	1 cut(s)	6
DpnI	GATC	4 cut(s)	116, 504, 639, 765
DpnII	GATC	4 cut(s)	114, 502, 637, 763
DriI	GACNNNNNGTC	1 cut(s)	223
Eam1104I	CTCTTC	1 cut(s)	172
Eam1105I	GACNNNNNGTC	1 cut(s)	223
EarI	CTCTTC	1 cut(s)	172
EciI	GGCGGA	1 cut(s)	220
Eco147I	AGGCCT	1 cut(s)	187
Eco31I	GGTCTC	2 cut(s)	77, 817
Eco47I	GGWCC	1 cut(s)	778
Eco57I	CTGAAG	3 cut(s)	95, 387, 395
EcoO109I	RGGNCCY	1 cut(s)	778
EcoRI	GAATTC	1 cut(s)	723
EcoRII	CCWGG	1 cut(s)	460
EcoT22I	ATGCAT	1 cut(s)	873
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
FaeI	CATG	7 cut(s)	330, 632, 648, 732, 799, 839, 875
FaqI	GGGAC	2 cut(s)	27, 191
FatI	CATG	7 cut(s)	326, 628, 644, 728, 795, 835, 871
FauI	CCCGC	1 cut(s)	477
FbaI	TGATCA	1 cut(s)	763
Fnu4HI	GCNGC	5 cut(s)	24, 27, 30, 707, 800
FokI	GGATG	2 cut(s)	311, 771
Fsp4HI	GCNGC	5 cut(s)	24, 27, 30, 707, 800
FspBI	CTAG	2 cut(s)	308, 540
GlaI	GCGC	3 cut(s)	6, 127, 129
GluI	GCNGC	5 cut(s)	24, 27, 30, 707, 800
GsaI	CCCAGC	1 cut(s)	372
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	2 cut(s)	187, 598
HgaI	GACGC	1 cut(s)	238
HhaI	GCGC	3 cut(s)	7, 128, 130
Hin1I	GRCGYC	2 cut(s)	5, 249
Hin1II	CATG	7 cut(s)	330, 632, 648, 732, 799, 839, 875
Hin6I	GCGC	3 cut(s)	5, 126, 128
HinP1I	GCGC	3 cut(s)	5, 126, 128
HincII	GTYRAC	2 cut(s)	553, 682
HindII	GTYRAC	2 cut(s)	553, 682
HindIII	AAGCTT	1 cut(s)	581
HinfI	GANTC	5 cut(s)	304, 413, 571, 669, 876
HphI	GGTGA	1 cut(s)	134
Hpy166II	GTNNAC	5 cut(s)	40, 79, 425, 553, 682
Hpy188I	TCNGA	3 cut(s)	114, 418, 889
Hpy188III	TCNNGA	9 cut(s)	76, 100, 118, 290, 478, 527, 623, 666, 836
Hpy8I	GTNNAC	5 cut(s)	40, 79, 425, 553, 682
Hpy99I	CGWCG	4 cut(s)	200, 242, 254, 272
HpyAV	CCTTC	4 cut(s)	65, 146, 403, 609
HpyCH4III	ACNGT	4 cut(s)	283, 448, 607, 714
HpyCH4IV	ACGT	3 cut(s)	42, 237, 457
HpyCH4V	TGCA	6 cut(s)	156, 709, 784, 799, 811, 871
HpyF10VI	GCNNNNNNNGC	4 cut(s)	29, 132, 365, 808
HpyF3I	CTNAG	1 cut(s)	119
HpySE526I	ACGT	3 cut(s)	42, 237, 457
Hsp92I	GRCGYC	2 cut(s)	5, 249
Hsp92II	CATG	7 cut(s)	330, 632, 648, 732, 799, 839, 875
HspAI	GCGC	3 cut(s)	5, 126, 128
KasI	GGCGCC	1 cut(s)	4
Ksp22I	TGATCA	1 cut(s)	763
Kzo9I	GATC	4 cut(s)	114, 502, 637, 763
LmnI	GCTCC	1 cut(s)	625
Lsp1109I	GCAGC	2 cut(s)	693, 811
LweI	GCATC	1 cut(s)	793
MaeI	CTAG	2 cut(s)	308, 540
MaeII	ACGT	3 cut(s)	42, 237, 457
MaeIII	GTNAC	1 cut(s)	442
MalI	GATC	4 cut(s)	116, 504, 639, 765
MboI	GATC	4 cut(s)	114, 502, 637, 763
MboII	GAAGA	7 cut(s)	85, 88, 101, 153, 159, 393, 542
MflI	RGATCY	2 cut(s)	114, 637
MhlI	GDGCHC	1 cut(s)	355
MluCI	AATT	5 cut(s)	436, 558, 617, 723, 842
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	2 cut(s)	298, 422
Mph1103I	ATGCAT	1 cut(s)	873
MroXI	GAANNNNTTC	2 cut(s)	380, 861
MseI	TTAA	1 cut(s)	579
MspR9I	CCNGG	1 cut(s)	462
MvaI	CCWGG	1 cut(s)	462
MvnI	CGCG	1 cut(s)	128
MwoI	GCNNNNNNNGC	4 cut(s)	29, 132, 365, 808
NarI	GGCGCC	1 cut(s)	5
NdeII	GATC	4 cut(s)	114, 502, 637, 763
NlaIII	CATG	7 cut(s)	330, 632, 648, 732, 799, 839, 875
NlaIV	GGNNCC	1 cut(s)	6
NmuCI	GTSAC	1 cut(s)	442
NsiI	ATGCAT	1 cut(s)	873
NspI	RCATGY	2 cut(s)	330, 632
NspV	TTCGAA	1 cut(s)	384
PagI	TCATGA	1 cut(s)	835
PalAI	GGCGCGCC	1 cut(s)	126
PaqCI	CACCTGC	1 cut(s)	153
PauI	GCGCGC	1 cut(s)	126
PceI	AGGCCT	1 cut(s)	187
PciI	ACATGT	1 cut(s)	326
PcsI	WCGNNNNNNNCGW	1 cut(s)	246
PdmI	GAANNNNTTC	2 cut(s)	380, 861
PfeI	GAWTC	3 cut(s)	571, 669, 876
PkrI	GCNGC	5 cut(s)	25, 28, 31, 708, 801
PleI	GAGTC	2 cut(s)	298, 421
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	2 cut(s)	298, 421
Ppu21I	YACGTR	1 cut(s)	43
PpuMI	RGGWCCY	1 cut(s)	778
PscI	ACATGT	1 cut(s)	326
Psp5II	RGGWCCY	1 cut(s)	778
Psp6I	CCWGG	1 cut(s)	460
PspFI	CCCAGC	1 cut(s)	368
PspGI	CCWGG	1 cut(s)	460
PspN4I	GGNNCC	1 cut(s)	6
PspPI	GGNCC	1 cut(s)	778
PspPPI	RGGWCCY	1 cut(s)	778
PstNI	CAGNNNCTG	1 cut(s)	118
PsuI	RGATCY	2 cut(s)	114, 637
PteI	GCGCGC	1 cut(s)	126
RsaI	GTAC	1 cut(s)	41
RsaNI	GTAC	1 cut(s)	40
SaqAI	TTAA	1 cut(s)	579
SatI	GCNGC	5 cut(s)	24, 27, 30, 707, 800
Sau3AI	GATC	4 cut(s)	114, 502, 637, 763
Sau96I	GGNCC	1 cut(s)	778
SchI	GAGTC	2 cut(s)	298, 422
ScrFI	CCNGG	1 cut(s)	462
SduI	GDGCHC	1 cut(s)	355
SfaNI	GCATC	1 cut(s)	793
SfoI	GGCGCC	1 cut(s)	6
SfuI	TTCGAA	1 cut(s)	384
SgsI	GGCGCGCC	1 cut(s)	126
SinI	GGWCC	1 cut(s)	778
SmlI	CTYRAG	1 cut(s)	354
SmoI	CTYRAG	1 cut(s)	354
Sse9I	AATT	5 cut(s)	436, 558, 617, 723, 842
SseBI	AGGCCT	1 cut(s)	187
SsiI	CCGC	5 cut(s)	24, 27, 30, 231, 484
SspDI	GGCGCC	1 cut(s)	4
SspMI	CTAG	2 cut(s)	308, 540
StuI	AGGCCT	1 cut(s)	187
StyD4I	CCNGG	1 cut(s)	460
TaaI	ACNGT	4 cut(s)	283, 448, 607, 714
TaiI	ACGT	3 cut(s)	45, 240, 460
TaqI	TCGA	4 cut(s)	240, 270, 384, 667
TasI	AATT	5 cut(s)	436, 558, 617, 723, 842
TauI	GCSGC	3 cut(s)	26, 29, 32
TfiI	GAWTC	3 cut(s)	571, 669, 876
Tru1I	TTAA	1 cut(s)	579
Tru9I	TTAA	1 cut(s)	579
TscAI	CASTG	2 cut(s)	453, 523
TseFI	GTSAC	1 cut(s)	442
TseI	GCWGC	2 cut(s)	706, 799
Tsp45I	GTSAC	1 cut(s)	442
TspDTI	ATGAA	2 cut(s)	336, 824
TspGWI	ACGGA	2 cut(s)	377, 629
TspRI	CASTG	2 cut(s)	453, 523
VpaK11BI	GGWCC	1 cut(s)	778
XapI	RAATTY	3 cut(s)	617, 723, 842
XceI	RCATGY	2 cut(s)	330, 632
XmnI	GAANNNNTTC	2 cut(s)	380, 861
XspI	CTAG	2 cut(s)	308, 540
Zsp2I	ATGCAT	1 cut(s)	873

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.