Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0002737

Physical Location & Seq

Genomic Coordinates Reverse (-)

29913147 .. 29916181

Gene Structure 3035 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1222200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 897 bp

ATGGCCGAGTTGACCCAGGGCGAGGTGGTCTACTCGCCCAAGACTCTTCAAGTCTGGCGAGCTCTGCTCAACTGGCTCGCCTTCTTCTCCCAGATCTTCCTCCAAATCCTCCGAGCTCTCGGTCACCACTCCCTCCTTTCTTCTTCCTCCTCCTCTTCCGCTTCTTCCGCCGCCTTCAAATCCCTGCCCGTCGAGCCGCCGGAGAACGACTCCCCCGCCGCCTCCGCCGTTGAAATCGCCGACACCCCCGATTCCGACGACCCTTTTGAAAAGCTCACGGTAGTTCTGGACCTGGATGAAACCCTAGTGTGTGCATATGAAACATCCGGCTTGCCGACTGTAGTTCGAACTCAAGCAACTGAAGGTGGGTTGAAGTGGTTTGAGCTTGAATGCGTATCTTCGGACAAGGAATGTGATGGCAAACCGAAGGTCAATTATGTCACAGTCTTTGAGCGTCCGGGGTTGCATGACTTCCTGAAACAAGTTAGTCAGTTTGCAGAGCTTGTGTTATTCACTGCTGGTCTTGAAGGTTATGCTAGACCACTTGTTGACAGAATAGATGTGGATAATCTGTTCAGTTATCGACTTTATCGGCCTTCAACCACCAGCACGGAATACCGGGAGCATGTGAAGGATCTATCTGGGCTATCAAAGGATATGAGGCGAATTGTTATTGTTGACAACAACCCCTTCAGTTTCTTGTTGCAACCTTTGAATGGAATTCCATGCATTCCATTTTCTGCCGGGCAAACACACGACAAACAGCTTTTGGATGTTCTTCTTCCACTCCTCAAGCAGCTCTCTCTGCAGAAAGATGTGAGGCCTGCCCTTTACGAAAGGTTTCACATGCCTGAATGGTTTCAAAAGCAGGGGATTCCTTCAACTGCGTGGAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0036211 GO:0042578 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NIF

Protein Analysis

299

Amino Acids

33.43

Weight (kDa)

5.13

Isoelectric Point (pI)

38.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NIF	PF03031	92 - 274	1.3e-42	NLI interacting factor-like phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002737)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55960
fragaria_vesca	FvH4_6g11400 FvH4_6g11400 FvH4_7g24520
malus_domestica	MD01G1153200.v1.1 MD04G1153700.v1.1 MD07G1222200.v1.1 MD12G1166900.v1.1
prunus_persica	Prupe.2G250400_v2.0.a1 Prupe.6G277800_v2.0.a1
pyrus_communis	pycom01g17080 pycom07g20080 pycom12g15890
rosa_chinensis	RchiOBHm_Chr1g0370271 RchiOBHm_Chr1g0370291 RchiOBHm_Chr3g0463021
rosa_laevigata	RLG00000024816 RLG00000027058 RLG00000027059 RLG00000027062
rosa_multiflora	Rmu_sc0000766.1_g000029 Rmu_sc0001657.1_g000001 Rmu_sc0006707.1_g000006 Rmu_sc0008124.1_g000028
rosa_roxburghii	Rroxscaffold_4G00286680 Rroxscaffold_4G00287340 Rroxscaffold_6G00417040
rosa_rugosa	Rorug01G0355600 Rorug03G0061100 Rorug03G0061200 Rorug03G0061200
rosa_samantha	Rh1AG363900 Rh1BG327000 Rh1BG327100 Rh1DG357400 Rh3AG118800 Rh3DG124700
rosa_wichuraiana	Rw1G032030 Rw3G010090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	30
AciI	CCGC	7 cut(s)	159, 168, 171, 197, 216, 219, 225
AclWI	GGATC	1 cut(s)	642
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	1 cut(s)	720
AcuI	CTGAAG	2 cut(s)	381, 676
AfiI	CCNNNNNNNGG	2 cut(s)	22, 716
AjnI	CCWGG	2 cut(s)	15, 291
AloI	GAACNNNNNNTCC	2 cut(s)	557, 589
AluBI	AGCT	7 cut(s)	62, 116, 274, 385, 502, 766, 799
AluI	AGCT	7 cut(s)	62, 116, 274, 385, 502, 766, 799
Alw21I	GWGCWC	2 cut(s)	64, 118
AlwI	GGATC	1 cut(s)	642
AoxI	GGCC	3 cut(s)	3, 593, 821
ApeKI	GCWGC	1 cut(s)	796
ApoI	RAATTY	1 cut(s)	720
ArsI	GACNNNNNNTTYG	4 cut(s)	248, 280, 414, 446
Asp700I	GAANNNNTTC	2 cut(s)	840, 858
AspS9I	GGNCC	1 cut(s)	289
AsuC2I	CCSGG	3 cut(s)	459, 620, 745
AsuHPI	GGTGA	1 cut(s)	116
AsuII	TTCGAA	1 cut(s)	346
AvaII	GGWCC	1 cut(s)	289
BanII	GRGCYC	2 cut(s)	64, 118
Bbv12I	GWGCWC	2 cut(s)	64, 118
BbvI	GCAGC	1 cut(s)	808
BccI	CCATC	1 cut(s)	410
BceAI	ACGGC	1 cut(s)	212
BciT130I	CCWGG	2 cut(s)	17, 293
BcnI	CCSGG	3 cut(s)	459, 620, 745
BfaI	CTAG	2 cut(s)	305, 537
BfmI	CTRYAG	2 cut(s)	339, 806
BglII	AGATCT	1 cut(s)	93
BisI	GCNGC	4 cut(s)	171, 197, 219, 797
BlsI	GCNGC	4 cut(s)	172, 198, 220, 798
Bme1390I	CCNGG	5 cut(s)	17, 293, 459, 620, 745
Bme18I	GGWCC	1 cut(s)	289
BmgT120I	GGNCC	1 cut(s)	289
BmrFI	CCNGG	5 cut(s)	17, 293, 459, 620, 745
BplI	GAGNNNNNCTC	4 cut(s)	51, 83, 194, 226
Bpu14I	TTCGAA	1 cut(s)	346
BpuEI	CTTGAG	2 cut(s)	336, 776
BpuMI	CCSGG	3 cut(s)	459, 620, 745
BsaJI	CCNNGG	3 cut(s)	15, 16, 458
Bsc4I	CCNNNNNNNGG	2 cut(s)	22, 716
Bse1I	ACTGG	1 cut(s)	77
BseBI	CCWGG	2 cut(s)	17, 293
BseDI	CCNNGG	3 cut(s)	15, 16, 458
BseGI	GGATG	3 cut(s)	301, 323, 778
BseLI	CCNNNNNNNGG	2 cut(s)	22, 716
BseNI	ACTGG	1 cut(s)	77
BseRI	GAGGAG	3 cut(s)	139, 142, 779
BseXI	GCAGC	1 cut(s)	808
BshFI	GGCC	3 cut(s)	5, 595, 823
BsiHKAI	GWGCWC	2 cut(s)	64, 118
BsiSI	CCGG	5 cut(s)	200, 327, 458, 619, 744
BslI	CCNNNNNNNGG	2 cut(s)	22, 716
BsmI	GAATGC	2 cut(s)	395, 729
BsnI	GGCC	3 cut(s)	5, 595, 823
Bsp119I	TTCGAA	1 cut(s)	346
Bsp1286I	GDGCHC	2 cut(s)	64, 118
Bsp143I	GATC	2 cut(s)	93, 634
BspACI	CCGC	7 cut(s)	159, 168, 171, 197, 216, 219, 225
BspANI	GGCC	3 cut(s)	5, 595, 823
BspMAI	CTGCAG	1 cut(s)	810
BspPI	GGATC	1 cut(s)	642
BspT104I	TTCGAA	1 cut(s)	346
BsrI	ACTGG	1 cut(s)	77
BssECI	CCNNGG	3 cut(s)	15, 16, 458
BssMI	GATC	2 cut(s)	93, 634
Bst2UI	CCWGG	2 cut(s)	17, 293
Bst4CI	ACNGT	3 cut(s)	280, 340, 445
Bst6I	CTCTTC	2 cut(s)	51, 160
BstBI	TTCGAA	1 cut(s)	346
BstC8I	GCNNGC	4 cut(s)	60, 78, 332, 825
BstEII	GGTNACC	1 cut(s)	122
BstF5I	GGATG	3 cut(s)	301, 323, 778
BstKTI	GATC	2 cut(s)	96, 637
BstMBI	GATC	2 cut(s)	93, 634
BstMWI	GCNNNNNNNGC	6 cut(s)	64, 73, 167, 193, 224, 805
BstNI	CCWGG	2 cut(s)	17, 293
BstNSI	RCATGY	2 cut(s)	629, 850
BstPI	GGTNACC	1 cut(s)	122
BstSCI	CCNGG	5 cut(s)	15, 291, 457, 618, 743
BstSFI	CTRYAG	2 cut(s)	339, 806
BstV1I	GCAGC	1 cut(s)	808
BstX2I	RGATCY	2 cut(s)	93, 634
BstYI	RGATCY	2 cut(s)	93, 634
BsuRI	GGCC	3 cut(s)	5, 595, 823
BtsCI	GGATG	3 cut(s)	301, 323, 778
BtsI	GCAGTG	1 cut(s)	513
BtsIMutI	CAGTG	1 cut(s)	513
Cac8I	GCNNGC	4 cut(s)	60, 78, 332, 825
Cfr13I	GGNCC	1 cut(s)	289
CseI	GACGC	1 cut(s)	443
CviAII	CATG	4 cut(s)	467, 626, 726, 847
DpnI	GATC	2 cut(s)	95, 636
DpnII	GATC	2 cut(s)	93, 634
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	2 cut(s)	51, 160
EarI	CTCTTC	2 cut(s)	51, 160
EciI	GGCGGA	2 cut(s)	157, 214
Ecl136II	GAGCTC	2 cut(s)	62, 116
Eco147I	AGGCCT	1 cut(s)	823
Eco24I	GRGCYC	2 cut(s)	64, 118
Eco47I	GGWCC	1 cut(s)	289
Eco53kI	GAGCTC	2 cut(s)	62, 116
Eco57I	CTGAAG	2 cut(s)	381, 676
Eco91I	GGTNACC	1 cut(s)	122
EcoICRI	GAGCTC	2 cut(s)	62, 116
EcoO65I	GGTNACC	1 cut(s)	122
EcoRI	GAATTC	1 cut(s)	720
EcoRII	CCWGG	2 cut(s)	15, 291
EcoT22I	ATGCAT	1 cut(s)	731
EcoT38I	GRGCYC	2 cut(s)	64, 118
FaeI	CATG	4 cut(s)	470, 629, 729, 850
FaiI	YATR	9 cut(s)	316, 318, 438, 468, 534, 627, 659, 727, 848
FatI	CATG	4 cut(s)	466, 625, 725, 846
FauI	CCCGC	1 cut(s)	223
FauNDI	CATATG	1 cut(s)	316
FblI	GTMKAC	1 cut(s)	30
Fnu4HI	GCNGC	4 cut(s)	171, 197, 219, 797
FokI	GGATG	3 cut(s)	308, 310, 785
FriOI	GRGCYC	2 cut(s)	64, 118
Fsp4HI	GCNGC	4 cut(s)	171, 197, 219, 797
FspBI	CTAG	2 cut(s)	305, 537
GluI	GCNGC	4 cut(s)	171, 197, 219, 797
HaeIII	GGCC	3 cut(s)	5, 595, 823
HapII	CCGG	5 cut(s)	200, 327, 458, 619, 744
HgaI	GACGC	1 cut(s)	443
Hin1II	CATG	4 cut(s)	470, 629, 729, 850
HincII	GTYRAC	3 cut(s)	12, 550, 679
HindII	GTYRAC	3 cut(s)	12, 550, 679
HinfI	GANTC	4 cut(s)	43, 209, 251, 874
HpaII	CCGG	5 cut(s)	200, 327, 458, 619, 744
HphI	GGTGA	1 cut(s)	116
Hpy166II	GTNNAC	4 cut(s)	12, 31, 550, 679
Hpy188I	TCNGA	3 cut(s)	113, 256, 403
Hpy188III	TCNNGA	3 cut(s)	287, 475, 524
Hpy8I	GTNNAC	4 cut(s)	12, 31, 550, 679
Hpy99I	CGWCG	2 cut(s)	194, 260
HpyAV	CCTTC	9 cut(s)	91, 184, 356, 421, 521, 606, 625, 700, 888
HpyCH4III	ACNGT	3 cut(s)	280, 340, 445
HpyCH4V	TGCA	6 cut(s)	314, 466, 497, 706, 729, 808
HpyF10VI	GCNNNNNNNGC	6 cut(s)	64, 73, 167, 193, 224, 805
Hsp92II	CATG	4 cut(s)	470, 629, 729, 850
Kzo9I	GATC	2 cut(s)	93, 634
LmnI	GCTCC	1 cut(s)	622
Lsp1109I	GCAGC	1 cut(s)	808
MaeI	CTAG	2 cut(s)	305, 537
MaeIII	GTNAC	2 cut(s)	122, 439
MalI	GATC	2 cut(s)	95, 636
MboI	GATC	2 cut(s)	93, 634
MflI	RGATCY	2 cut(s)	93, 634
MhlI	GDGCHC	2 cut(s)	64, 118
MluCI	AATT	3 cut(s)	433, 666, 720
MlyI	GAGTC	2 cut(s)	37, 203
MmeI	TCCRAC	1 cut(s)	279
Mph1103I	ATGCAT	1 cut(s)	731
MroXI	GAANNNNTTC	2 cut(s)	840, 858
MspI	CCGG	5 cut(s)	200, 327, 458, 619, 744
MspR9I	CCNGG	5 cut(s)	17, 293, 459, 620, 745
Mva1269I	GAATGC	2 cut(s)	395, 729
MvaI	CCWGG	2 cut(s)	17, 293
MwoI	GCNNNNNNNGC	6 cut(s)	64, 73, 167, 193, 224, 805
NciI	CCSGG	3 cut(s)	459, 620, 745
NdeI	CATATG	1 cut(s)	316
NdeII	GATC	2 cut(s)	93, 634
NlaIII	CATG	4 cut(s)	470, 629, 729, 850
NmeAIII	GCCGAG	1 cut(s)	31
NmuCI	GTSAC	2 cut(s)	122, 439
NsiI	ATGCAT	1 cut(s)	731
NspI	RCATGY	2 cut(s)	629, 850
NspV	TTCGAA	1 cut(s)	346
PasI	CCCWGGG	1 cut(s)	16
PceI	AGGCCT	1 cut(s)	823
PctI	GAATGC	2 cut(s)	395, 729
PdmI	GAANNNNTTC	2 cut(s)	840, 858
PfeI	GAWTC	2 cut(s)	251, 874
PkrI	GCNGC	4 cut(s)	172, 198, 220, 798
PleI	GAGTC	2 cut(s)	37, 203
PpsI	GAGTC	2 cut(s)	37, 203
Psp124BI	GAGCTC	2 cut(s)	64, 118
Psp6I	CCWGG	2 cut(s)	15, 291
PspEI	GGTNACC	1 cut(s)	122
PspGI	CCWGG	2 cut(s)	15, 291
PspPI	GGNCC	1 cut(s)	289
PstI	CTGCAG	1 cut(s)	810
PsuI	RGATCY	2 cut(s)	93, 634
SacI	GAGCTC	2 cut(s)	64, 118
SatI	GCNGC	4 cut(s)	171, 197, 219, 797
Sau3AI	GATC	2 cut(s)	93, 634
Sau96I	GGNCC	1 cut(s)	289
SchI	GAGTC	2 cut(s)	37, 203
ScrFI	CCNGG	5 cut(s)	17, 293, 459, 620, 745
SduI	GDGCHC	2 cut(s)	64, 118
SfcI	CTRYAG	2 cut(s)	339, 806
SfuI	TTCGAA	1 cut(s)	346
SinI	GGWCC	1 cut(s)	289
SmlI	CTYRAG	2 cut(s)	351, 791
SmoI	CTYRAG	2 cut(s)	351, 791
Sse9I	AATT	3 cut(s)	433, 666, 720
SseBI	AGGCCT	1 cut(s)	823
SsiI	CCGC	7 cut(s)	159, 168, 171, 197, 216, 219, 225
SspMI	CTAG	2 cut(s)	305, 537
SstI	GAGCTC	2 cut(s)	64, 118
StuI	AGGCCT	1 cut(s)	823
StyD4I	CCNGG	5 cut(s)	15, 291, 457, 618, 743
TaaI	ACNGT	3 cut(s)	280, 340, 445
TaqI	TCGA	3 cut(s)	192, 346, 583
TaqII	GACCGA	1 cut(s)	110
TasI	AATT	3 cut(s)	433, 666, 720
TauI	GCSGC	3 cut(s)	173, 199, 221
TfiI	GAWTC	2 cut(s)	251, 874
TscAI	CASTG	1 cut(s)	520
TseFI	GTSAC	2 cut(s)	122, 439
TseI	GCWGC	1 cut(s)	796
Tsp45I	GTSAC	2 cut(s)	122, 439
TspDTI	ATGAA	2 cut(s)	312, 333
TspGWI	ACGGA	1 cut(s)	626
TspRI	CASTG	1 cut(s)	520
VpaK11BI	GGWCC	1 cut(s)	289
XapI	RAATTY	1 cut(s)	720
XceI	RCATGY	2 cut(s)	629, 850
XmiI	GTMKAC	1 cut(s)	30
XmnI	GAANNNNTTC	2 cut(s)	840, 858
XspI	CTAG	2 cut(s)	305, 537
Zsp2I	ATGCAT	1 cut(s)	731

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.